Food Bioscience
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2024
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IF 4.8
Yan Liang,Chunling Lai,Liangliang Gao,Qingna Wu,Meiling Huang,Yueming Jiang,Jun Zhang
This paper presented the initial investigation into the impact of Penicillium polonicum fermentation on the functional components and bioactivities of navel orange peel. The fermentation process resulted in a notable enhancement of the total flavonoid content (TFC), total phenolic content (TPC), antioxidant capacity, antibacterial activity, and antiglycation activity of orange peel. Eighteen flavonoids were isolated and identified from the ethanolic extract of fermented orange peel (FEE) by employing chromatographic and spectroscopic techniques. The results of the HPLC analysis showed that three flavanones (naringenin, hesperetin, and isosakuranetin) were newly produced and present in high concentrations in FEE, with hesperetin being the most abundant component (39.74 mg/g) in comparison to the ethanolic extract of unfermented orange peel (EE). The TPC, TFC, antioxidant, and antiglycation activities of FEE, as well as the enzymatic activity of β-glucosidase in the fermented orange peels, showed a gradual increase with the progression of fermentation time over a five-month period. HPLC analysis revealed that the fermentation metabolites generated by P. polonicum in rice and orange peel media exhibited distinct profiles, indicating that the fermentation of orange peel by P. polonicum did not result in the production of potential mycotoxins. The findings of this study provided a scientific basis for the high-value utilization of navel orange peels and established a reference point for the potential applications of other peel byproducts via microbial fermentation.
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ARCHIVES OF PHARMACAL RESEARCH
·
2016
·Vol. 39
·期 6
·762-770
·
IF 2.49
Wang Nan,Cui Cheng-Bin,Li Chang-Wei
A novel cyclic dipeptide, named penicimutide ( 1 ), and four known cyclic dipeptides, cyclo( l -Val- l -Pro) ( 2 ), cyclo( l -Ile- l -Pro) ( 3 ), cyclo( l -Leu- l -Pro) ( 4 ) and cyclo( l -Phe- l -Pro) ( 5 ), were isolated from a neomycin-resistant mutant of the marine-derived fungus Penicillium
purpurogenum G59. The structure of 1 , including the absolute configuration, was determined by spectroscopic and chemical methods, especially NMR and Marfey’s analysis. An unusual amino acid in 1 , 4,5-didehydro- l -leucine, was found for the first time occurring in nature. HPLC–ESI–MS analysis evidenced that 1 – 3 were produced only in the mutant strain, but 4 and 5 were produced in both the mutant and parental strains, indicating that the introduction of neomycin-resistance in the mutant activated pathways of 1 – 3 biosynthesis that were silent in the parental strain. Compound 1 selectively inhibited HeLa cells (among five tested human cancer cell lines) with an inhibition rate (IR %) of 39.4 % at 100 µg/mL, a similar inhibition intensity to that of the positive control 5-fluorouracil (IR % of 41.4 % at 100 µg/mL against HeLa cells). The present work exemplifies the effectiveness of our previous DMSO-mediated method for introducing drug-resistance in fungi to activate silent biosynthetic pathways to obtain new bioactive compounds.
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高影响因子
FOOD CONTROL
·
2016
·
IF 3.388
Zhiyong Zhao,Xianli Yang,Xiaoyan Zhao,Bing Bai,Chunxia Yao,Na Liu,Jianhua Wang,Changyan Zhou
The presence of Aspergillus and Penicillium species during the fermentation process of rice wine is inevitable. The toxic secondary metabolites produced by the two fungi need to be practically monitored for rice wine safety. In this study, we developed a fast and simple vortex-assisted dispersive liquid-liquid microextraction (VADLLME) for the extraction and preconcentration of major Aspergillus and Penicillium mycotoxins (aflatoxin B1, aflatoxin B2, aflatoxin G1, aflatoxin G2, ochratoxin A, mycophenolic acid, sterigmatocystin and verruculogen) in rice wine. The key parameters affecting the extraction efficiency of VADLLME procedure (pH value, type and volume of extraction and dispersive solvents) were optimized by response surface methodology (RSM) combined with Box-Behnken design (BBD) and desirability function. Target compounds were analyzed by liquid chromatography tandem mass spectrometry (LC-MS/MS). Under the optimum conditions, good linearity ( R 2 >0.99) was achieved for all the analytes in the concentration range of 0.5–100 μg/L. The recoveries for spiked samples at three concentration levels were between 84.5% and 111.2%, with the relative standard deviations (RSD) lower than 20%. The limits of detection (LOD) and quantification (LOQ) ranged from 0.05 to 0.5 μg/L and from 0.1 to 1.0 μg/L, respectively. Simplicity in operation, low cost and low usage of organic solvents were the major advantages of the developed method. The method was successfully applied to the analysis of rice wine samples. It was found that mycophenolic acid had high incidence rate in rice wine, which needed to be continuously monitored.
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POSTHARVEST BIOLOGY AND TECHNOLOGY
·
2023
·
IF 7
Guliang Yang,Zhiwen Qi,Sijie Shan,Kun Lu,Junqin Zhou,Liying Yang,Xiaofeng Tan
In this study, we developed a method for synthesizing silver nanoparticles (AgNPs) using aqueous assembly assay and anchoring them with camellia seed cake polyphenols (CPs). Zeta potential analysis demonstrated a higher binding affinity of CP-AgNPs to Penicillium digitatum , which could be attributed to the interaction between the hydroxyl group of CP-AgNPs and the polysaccharide present on the fungal cell wall. Compared to AgNPs, the CP-AgNPs outperformed conventional AgNPs in antifungal activity against P. digitatum . The mechanism underlying this antimicrobial effect involves raising the level of oxidative stress by regulating the expression levels of oxidative stress response genes. In addition, CP-AgNPs/CS showed highly effective in reducing citrus decay levels during chamber storage. In conclusion, the use of CPs as anchoring agent in the in-situ aqueous assembly of CP-AgNPs improved their biocompatibility and antimicrobial properties. CP-AgNPs hold great promise for applications in fruit preservation, especially citrus preservation.
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Innovative Food Science & Emerging Technologies
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2023
·
IF 6.6
Xiao-Jia He,Jiao Jiao,Qing-Yan Gai,Jin-Xian Fu,Yu-Jie Fu,Zi-Yi Zhang,Jie Gao
A novel endophytic fungus Penicillium rubens isolated from pigeon pea producing cellulase (0.73 ± 0.14 U/mL) and β-glucosidase (4.85 ± 0.42 U/mL) was used to ferment pigeon pea roots (PPR) for obtaining bioactive genistein . Semi-solid-state fermentation (SSSF) with water was found to be the appropriate strategy for increasing genistein level in PPR. The genistein yield (0.37 ± 0.02 mg/g) in PPR fermented by P. rubens under the optimal conditions was 3.08-fold higher than that in the control (0.12 ± 0.02 mg/g). Scanning electron microscope results indicated that P. rubens utilized the nutrients in PPR for growth and could significantly destroy cell walls, thus contributing to the release of more genistein. The bioactivities of extracts from PPR fermented by P. rubens were higher than control. The present study provided a cost-effective SSSF approach to achieve higher yield of genistein from agrifood by-products PPR using the novel P. rubens . Industrial relevance A novel endophytic fungus P. rubens , capable of cell wall hydrolysis and genistin deglycosylation , was used to ferment PPR using SSSF strategy to obtain higher yield of bioactive genistein. The present study developed a method associated with advantages of simple operation, low cost, low energy consumption and environmental friendliness for the effective utilization of agrifood by-products PPR in food , nutraceutical, and other industrial fields. Also, P. rubens exhibited promising potential in fermentation fields for improving yields of other bioactive aglycones from food and medicinal resources.
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MicrobiologyOpen
·
2017
·Vol. 6
·期 4
·e00485
·
IF 2.747
Xin Feng,Jian Xu,Yu Liang,Guo-Li Chen,Xian-Wei Fan,You-Zhi Li
Graphical Our work not only provides the potential protein biomarkers indicating the Cu pollution, target metabolic steps for engineering the Cu-tolerant fungi during bioremediation, but also presents clues for insight into heavy metal tolerance mechanisms of other eukaryotes. Filamentous fungi-copper (Cu) interactions are very important in the formation of natural ecosystems and the bioremediation of heavy metal pollution. However, important issues at the proteome level remain unclear. We compared six proteomes from Cu-resistant wild-type (WT) Penicillium janthinellum strain GXCR and a Cu-sensitive mutant (EC-6) under 0, 0.5, and 3 mmol/L Cu treatments using iTRAQ. A total of 495 known proteins were identified, and the following conclusions were drawn from the results: Cu tolerance depends on ATP generation and supply, which is relevant to glycolysis pathway activity; oxidative phosphorylation, the TCA cycle, gluconeogenesis, fatty acid synthesis, and metabolism are also affected by Cu; high Cu sensitivity is primarily due to an ATP energy deficit; among ATP generation pathways, Cu-sensitive and Cu-insensitive metabolic steps exist; gluconeogenesis pathway is crucial to the survival of fungi in Cu-containing and sugar-scarce environments; fungi change their proteomes via two routes (from ATP, ATP-dependent RNA helicases (ADRHs), and ribosome biogenesis to proteasomes and from ATP, ADRHs to spliceosomes and/or stress-adapted RNA degradosomes) to cope with changes in Cu concentrations; and unique routes exist through which fungi respond to high environmental Cu. Further, a general diagram of Cu-responsive paths and a model theory of high Cu are proposed at the proteome level. Our work not only provides the potential protein biomarkers that indicate Cu pollution and targets metabolic steps for engineering Cu-tolerant fungi during bioremediation but also presents clues for further insight into the heavy metal tolerance mechanisms of other eukaryotes.
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Food Bioscience
·
2025
·
IF 5.9
Lijun Ling,Fanjin Kong,Yijuan Jiang,Wenyue Zhang,Rongxiu Mo,Rui Yue,Yao Li,Lijun Feng,Yongpeng Zhou
Citrus reticulata 'Shiyue Ju' are prone to fungal infections because of their high maturity, water content, and remote cultivation locations. Chemical control, though effective, leaves fruit residues that endanger health and the environment. Endophytic VOCs potently inhibit pathogens with high efficacy, low cost, and minimal ecological impact, positioning them as promising natural post-harvest preservatives. In this study, we isolated the pathogenic fungus ( Penicillium italicum Q-1) from rotting Citrus reticulata 'Shiyue Ju' and used Q-1 as the test strain to screen for endophytic fungi Y-4 isolated from healthy Citrus reticulata 'Shiyue Ju' that can produce VOCs with strong antagonistic activity. We analyzed the VOC components and screened for the highly bactericidal activity compound 3-pentene-2-one,exhibiting complete (100 %) growth inhibition at 4 μL/L. We also investigated the inhibitory activity and mechanisms of action of the VOCs and antifungal compounds. The results indicated that the VOCs produced by Y-4 and 3-pentene-2-one could inhibit the growth and reproduction of the pathogenic fungus, primarily by disrupting the normal morphological structure of hyphae and spores and damaging the integrity of the cell membrane. Furthermore, 3-pentene-2-one at appropriate concentrations can inhibit the spread of pathogenic fungi on Citrus reticulata 'Shiyue Ju' and effectively delay the decline in fruit quality. These findings indicate that 3-pentene-2-one is a powerful tool for controlling P. italicum and provides a theoretical basis for post-harvest disease management of Citrus reticulata 'Shiyue Ju' .
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高影响因子
JOURNAL OF APPLIED MICROBIOLOGY
·
2025
·
IF 3.2
Luo Yuwei,Zhang Yonghua,Chen Xiumei,Che Jinxin,Tao Nengguo
AimsTo overcome the hydrophobicity and effumability restriction of the essential oil in practice, we aim to fabricate the (E)-2-octenal-β-cyclodextrin inclusion complex (O/β-CDIC) and investigate its encapsulation mechanism.Methods and ResultsThe O/β-CDIC was fabricated using saturated aqueous solution method combined with freeze-dried method. The encapsulation efficiency and loading content of O/β-CDIC were 45.61 ± 1.37% and 2.97 ± 0.08%. In vitro assays, the MIC (Minimum Inhibitory Concentration) and MFC (Minimum Fungicidal Concentration) of the O/β-CDIC were 2.00 and 8.00 mg·mL⁻¹ for P. italicum XX5 mycelial growth, respectively. In addition, the O/β-CDIC effectively reduced the incidence of blue mold in citrus fruit and maintained fruit quality. The detailed structure characterization combined with the molecular docking study, molecular electrostatic potential, and independent gradient model based on Hirshfeld analysis collectively demonstrated that (E)-2-octenal was entrapped successfully within the β-CD cavity.ConclusionsThe O/β-CDIC significantly enhanced the thermal stability and phase solubility of (E)-2-octenal while effectively preserving its antifungal activity. Characterization analyses confirmed the presence of non-covalent interactions between β-CD and (E)-2-octenal.
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高影响因子
INDUSTRIAL CROPS AND PRODUCTS
·
2023
·
IF 5.9
Xiao-Jia He,Jin-Xian Fu,Jiao Jiao,Qing-Yan Gai,Yu-Jie Fu,Xue Feng,Yuan Wang
Resveratrol (a high-value natural polyphenolic compound with numerous pharmacological activities) is predominantly present in the form of the glycoside polydatin in Polygonum cuspidatum roots. In this study, a novel endophytic fungus, Penicillium rubens , capable of producing both cellulase (0.71 ± 0.09 U/mL) and β-glucosidase (4.35 ± 0.38 U/mL) for cell wall hydrolysis and polydatin deglycosylation, was used to ferment P. cuspidatum roots to improve the yield of valuable resveratrol. Results showed that the semi-solid-state fermentation (SSSF) using the homogeneous cultures of P. rubens was the appropriate strategy to obtain a higher yield of resveratrol from P. cuspidatum roots. Under the optimal fermentation conditions (temperature 30 °C, inoculation dose 4.0 mL/g, and time 72 h), the yield of resveratrol (19.95 ± 0.83 mg/g DW) in P. cuspidatum roots fermented by P. rubens was 19.18-fold higher than that in the unfermented control (1.04 ± 0.47 mg/g DW). Moreover, the digestibility of P. cuspidatum roots by P. rubens in terms of dry matter weight and cellulose content was 40.04% and 21.91%, respectively. Scanning electron microscope observation indicated that P. rubens fermentation could effectively disrupt the cell walls of P. cuspidatum roots, thus contributing to the release of more polydatin for deglycosylation. In addition, the extracts of P. cuspidatum roots fermented by P. rubens exhibited higher antioxidant and antibacterial activities compared with those of the unfermented control. The economic analysis showed that the benefit of SSSF approach for resveratrol production was much greater than that of the unfermented control. Overall, this study proposed a cost-effective SSSF method using the novel P. rubens to obtain bioactive resveratrol from P. cuspidatum roots, which had promising potential in the pharmaceutical, nutraceutical , and cosmetic industries.
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