APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
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2014
·Vol. 99
·期 7
·3211-3220
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IF 3.811
Wang Xiangqian,Wu Chao,Liu Nan,Li Sujing,Li Wei,Chen Jianmeng,Chen Dongzhi
A Pseudomonas sp. strain WL2 that is able to efficiently metabolize ethyl mercaptan (EM) into diethyl disulfide (DEDS) through enzymatic oxidation was isolated from the activated sludge of a pharmaceutical wastewater plant. One hundred percent removal of 113.5 mg L −1 EM and 110.3 mg L −1 DEDS were obtained within 14 and 32 h, respectively. A putative EM degradation pathway that involved the catabolism via DEDS was proposed, which indicated DEDS were further mineralized into carbon dioxide (CO 2 ), bacterial cells, and sulfate (SO 4
2− ) through the transformation of element sulfur and ethyl aldehyde. Degradation kinetics for EM and DEDS with different initial concentrations by strain WL2 were evaluated using Haldane-Andrews model with maximum specific degradation rates of 3.13 and 1.33 g g −1 h −1 , respectively, and maximum degradation rate constants of 0.522 and 0.175 h −1 using pseudo-first-order kinetic model were obtained. Results obtained that aerobic degradation of EM by strain WL2 was more efficient than those from previous studies. Substrate range studies of strain WL2 demonstrated its ability to degrade several mercaptans, disulfides, aldehydes, and methanol. All the results obtained highlight the potential of strain WL2 for the use in the biodegradation of volatile organic sulfur compounds (VOSCs).
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Frontiers in Bioengineering and Biotechnology
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2022
·
IF 6.064
Ma Anzhou,Ma Jianpeng,Chen Xianke,Zhuang Guoqiang
Highly chlorinated benzenes usually have a low efficient degradation in environment. Here we proposed a synergistic removal strategy of pentachlorobenzene (PeCB) using Pseudomonas sp. JS100 coupled with immobilized nanoscale zero-valent iron (NZVI). The structural and textural features of the synergistic system were characterized by X-ray powder diffraction, field emission scanning electron microscopy, and a specific surface area and pore size analysis. Nanoscale zero-valent iron particles were dispersed and attached to the biofilter, which increased the specific surface area to 34.5 m2 g−1. The batch experiment revealed that the removal efficiency of PeCB reached 80.2% in the synergistic system within 48 h. The degradation followed pseudo-first-order reaction kinetics, and the reaction rate constant was measured to be 0.0336 h−1. In the degradation mechanism, PeCB was degraded by NZVI to lower chlorobenzenes, which were utilized by Pseudomonas sp. JS100 as nutrients, thereby achieving rapid removal of PeCB.
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BIOELECTROCHEMISTRY
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2025
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IF 4.5
Maryam Allahyari,Emre Dokuzparmak,Arzum Erdem,Alper AKKAYA,Bahattin Tanyolac
Pseudomonas aeruginosa is a major pathogen responsible for severe infections, particularly in immunocompromised patients and those with chronic conditions. Its increasing resistance to antibiotics underscores the urgent need for effective detection systems. This study introduces a novel biosensor based on bacteriophage (M-PAP1) in combination with MWCNT-COOH modified SPE and its application for the sensitive identification of Pseudomonas aeruginosa . The phage provides high specificity and strong target binding, while the combined ECL and EIS modes offer complementary signal outputs, enhancing analytical reliability and reducing false responses. The ECL sensor achieved LoD of 0.755 CFU ml −1 and demonstrated a broad linear working range of 2.28 to 10 10 CFU ml −1 (R 2 : 0.9981). In artificial urine samples, the sensor demonstrated a recovery rate of 92% to 97%, indicating its effectiveness in real biological matrices. The dual functionality for both ECL and EIS measurements highlights the system's versatility and potential for real-time clinical applications. The system also exhibited exceptional selectivity and minimal interference from non-specific bacteria such as Escherichia coli and Pseudomonas putida and Pseudomonas fluorescens. Overall, the dual-mode strategy significantly strengthens diagnostic accuracy by enabling cross-validated detection signals, offering a robust platform for rapid monitoring of P. aeruginosa in the context of rising antimicrobial resistance.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2020
·
IF 4.192
Miaomiao Li,Jinlong Song,Qingyun Ma,Delong Kong,Yiqing Zhou,Xu Jiang,Rebecca Parales,Zhiyong Ruan,Qinghua Zhang
A total of five strains of nicosulfuron-degrading bacteria were isolated from a continuously cultivated microbial consortium using culturomics. Among them, a novel Pseudomonas strain, LAM1902, with the highest degradation efficiency was investigated in detail. The characteristics of nicosulfuron-degradation by LAM1902 were investigated and optimized by response surface analysis. Furthermore, non-targeted metabolomic analysis of extracellular and intracellular biodegradation of nicosulfuron by LAM1902 was carried out by liquid chromatography/mass spectroscopy (LC–MS) and gas chromatography–time-of-flight/mass spectroscopy (GC–TOF/MS). It was found that nicosulfuron was degraded by LAM1902 mainly via breaking the sulfonylurea bridge, and this degradation might be attributed to oxalate accumulation. The results of GC–TOF/MS also showed that the intracellular degradation of nicosulfuron did not occur. However, nicosulfuron exerted a significant influence on the metabolism of inositol phosphate, pyrimidine, arginine/proline, glyoxylate, and dicarboxylate metabolism and streptomycin biosynthesis. The changes of myo-inositol, trehalose, and 3-aminoisobutanoic acid were proposed as a mechanism of self-protection against nicosulfuron stress.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2026
·
IF 6.7
Zhaojia Li,Xin Luo,Na Tao,Shuai Zhu,Sheng Wang,Jing Liu,Moxian Chen,Jingjiang Zhou,Xiangyang Li
Abstract Pseudomonas syringae pv. actinidiae (Psa) is a devastating kiwifruit pathogen causing severe economic losses. In this study, Xinjunan (dioctyldiethylenetriamine) exerts potent antibacterial activity against Psa (EC50 = 4.29 mg/L), outperforming thiodiazole-copper (34.8 mg/L). Transcriptomic analysis showed that Xinjunan affected pathways associated with flagellar assembly, biofilm formation, membrane biogenesis, and chemotaxis. Xinjunan treatment also induced reactive oxygen species (ROS) accumulation, leading to bacterial death. Deletion of FleQ enhances Psa resistance to Xinjunan, reduces virulence, and inhibits flagellar formation. Under Xinjunan treatment, the mutant strain showed better growth than the wild-type strain, with no significant differences in motility capacity or ROS levels; the complemented strain restored sensitivity to Xinjunan. Microscale thermophoresis and isothermal titration calorimetry determined dissociation constants of Xinjunan binding to the FleQ protein as 1.64 and 6.41 μM, respectively. Molecular docking and molecular dynamics simulations identified FleQ Glu264 as a critical binding residue. These findings provide promising targets for the management of bacterial phytopathogen Psa.
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JOURNAL OF HAZARDOUS MATERIALS
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2026
·
IF 10.6
Siming Jiang,Honghai Yuan,Zhibiao Chen,Zuliang Chen
Ammonium, typically a pollutant, accelerates rare earth recovery by indigenous bacteria. • One-step treatment achieves over 90% REE recovery and 76% nitrogen removal in 34 h. • Extracellular complexation and biomineralization dominate REE fixation mechanism. • NH4 + -N promotes bacterial growth, EPS secretion, and additional REE binding sites. • Converts ammonium-rich rare earth wastewater into sustainable metal resources. The mining process of ion-adsorption-type rare earth ores generates a large amount of wastewater containing rare earth elements (REEs) and high concentrations of ammonium nitrogen (NH + 4 -N). It is urgent to develop single-stage, low-energy technologies to achieve REEs recovery and NH + 4 -N removal simultaneously. Here, we present a local Pseudomonas oryzihabitan , which is capable of achieving this goal. Interestingly, the typical pollutant ammonium does accelerate the recovery of REEs. Comprehensive characterizations show that the fixation of REEs mainly occurs extracellularly through surface complexation and biomineralization. The acceleration of REE recovery by NH + 4 -N may involve multiple mechanisms, including supporting rapid bacterial growth, enhancing extracellular organic secretion, and promoting biomineralization. Importantly, this acceleration is specific to ammonium and cannot be replicated by other nitrogen sources supporting comparable biomass. This strain was able to recover over 90% of the REEs and remove 76.2% of NH + 4 -N from real mine wastewater within 34 h, suggesting its potential as a starting point for bioremediation strategies. This work provides a conceptual basis for designing single-reactor bioprocesses that can convert wastewater from rare earth mines rich in NH + 4 -N into sustainable metal resources. Download: Download high-res image (184KB) Download: Download full-size image
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JOURNAL OF ETHNOPHARMACOLOGY
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2026
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IF 6.8
Jinling Zhang,Zhenzhen Wang,Shangtao Wang,Yuqi Yan,Xiaoyu Wang,Man Zhang,Yuanyuan Hou,Gang Bai
Ethnopharmacological relevance Codonopsis Radix (CR), known as Dangshen in traditional Chinese medicine,is frequently used to tonify Qi and strengthen the spleen and lung. Clinically, "Lung- Qi deficiency" manifests as respiratory weakness and susceptibility to infection. The traditional action of "consolidating Qi to protect the exterior" parallels the physiological preservation of the pulmonary endothelial barrier. However, the bioactive basis linking this traditional efficacy to barrier reinforcement remains unclear. Aim of the study This study evaluates the protective effects of CR against Pseudomonas aeruginosa -induced acute lung injury (ALI), identifies the primary bioactive component responsible for barrier protection, and elucidates its molecular mechanism involving cytoskeletal stabilization. Materials and methods A P. aeruginosa -induced ALI mouse model was established to assess the efficacy of CR extract (CRE) and its polyacetylene glycoside, lobetyolin (LBT). Lung injury severity, inflammatory responses, and endothelial barrier function were systematically evaluated. Bioactivity-guided fractionation screened for active constituents. The mechanism was investigated using integrated transcriptomics, chemical proteomics, and molecular docking, validated by in vitro and in vivo experiments. Results Both CRE and LBT significantly attenuated lung histopathology, diminished pulmonary edema, and suppressed leukocyte infiltration and pro-inflammatory cytokine production. LBT was identified as the key bioactive constituent restoring endothelial barrier integrity, evidenced by upregulated expression and junctional localization of VE-Cadherin, ZO-1, and Claudin-5. Mechanistically, LBT directly targets α-actinin-1 (ACTN1). Functioning as a "molecular stabilizer", LBT enhances ACTN1-F-actin affinity, promoting cortical actin organization and stabilizing intercellular junctions under inflammatory stress. Conclusions This study identifies LBT as a novel structural consolidator of the vascular endothelial barrier. By demonstrating that LBT reinforces endothelial cytoskeletal organization via ACTN1, our findings offer a compelling modern scientific interpretation of the traditional efficacy of CR in "tonifying Lung- Qi ": physically strengthening the biological barrier. Furthermore, targeting ACTN1-mediated cytoskeleton modulation presents a promising strategy for treating ALI and vascular leakage disorders.
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ENVIRONMENTAL RESEARCH
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2026
·
IF 8.2
Bin Dong,Chengxu Lai,Mengwei Han,Sanping Zhao,Songhao Luo,Yongbing Zhu
2,4,6-Trinitrotoluene (TNT) contamination presents serious threats to ecological safety and human health due to its high toxicity, carcinogenicity, and environmental persistence. Although microbial bioremediation is eco-friendly and cost-effective, its efficiency is often limited by low removal rates and weak microbial tolerance under TNT stress. This study utilized biochar to facilitate microbial biotransformation of TNT and systematically elucidated its synergistic mechanisms. Four types of biochar from different waste sources were screened, among which the wood biochar (MBC) exhibited the optimal performance. With MBC amendment, the TNT removal efficiency increased from 46.2% (strain-only control) to 88.7% within 48 h at an initial TNT concentration of 100 mg/L. Meanwhile, the first-order kinetic rate constant rose from 0.01323 h −1 (strain-only) to 0.03987 h −1 , revealing that MBC greatly accelerated the TNT removal rate. Biochar accelerated removal kinetics, alleviated TNT-induced growth inhibition of strain T22 via lowering aqueous TNT concentration, and stimulated extracellular polymeric substance secretion. Untargeted metabolomics revealed 330 up-regulated differential metabolites in the biochar-microbe system, indicating intensive metabolic reprogramming. Mechanistically, MBC directly enhanced TNT transformation via activation of the nitrotoluene degradation and cofactor biosynthesis pathways. The synergistic mechanisms were proposed to include: (1) weakened TNT exposure; (2) enhanced antioxidant metabolism; (3) activated core transformation pathways; and (4) supplemented basal metabolic supply. Although complete mineralization and soil-scale validation are beyond the present scope, our findings establish a mechanistic foundation for designing biochar-microbe systems for enhanced nitroaromatic remediation.
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WATER RESEARCH
·
2026
·
IF 12.8
Yunxia Zu,Xueqi Chen,Zimeng Zhang,Aijie Wang,Zhiling Li
Organohalides and heavy metals often co-contaminate groundwater, however, the biotoxicity of heavy metal strongly inhibits microbial reductive dechlorination activity and existing bioremediation strategies seldom achieve simultaneous removal of both types of pollutants. Here, we demonstrated that cysteine amendment enabled synchronous reductive dechlorination and cadmium (Cd(II)) removal in an organohalide-respiring bacterium Pseudomonas sp. CP-1 through cysteine-mediated sulfide bioprecipitation. Batch experiments revealed that cysteine supplementation at a Cd(II):Cys molar ratio of 1:2 achieved an enhanced 2,4,6-trichlorophenol dechlorination kinetics ( k d = 0.28 d -1 ) and 91.20% recovery of Cd(II) as nanocrystalline CdS deposited on the cell surface. In simulated continuous-flow biobarriers, the addition of cysteine sustained high removal efficiencies for both contaminants despite hydraulic fluctuations, with a highest dechlorination rate reaching 120 µM·(L·d) -1 , 9.6 times higher than the pure dechlorination process. Multi-omics analysis revealed the potential coupled metabolic mechanism of dechlorination and Cd(II) removal in which the amendment of cysteine not only activated cysteine desulfurase to produce H 2 S for extracellular precipitation of CdS, but also generated alanine and pyruvate, which likely assimilated into the TCA cycle to augment NADH production and electron supply for reductive dechlorination. This work establishes a unique single-strain based strategy for synergistic remediation of organohalide and heavy metal ions in groundwater, highlighting the potential of cysteine-driven metabolism as an effective bioaugmentation tool for sustainable remediation of organohalide and heavy metal co-contaminated groundwater.
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PEST MANAGEMENT SCIENCE
·
2026
·
IF 4.2
Jia Xiao,Xiufang Deng,Wei Zhao,Lingyun Zhang,Keying Xie,Lei Chen,Jiaqi Wan,Hongdong Liao
BACKGROUND Globally restricted high-toxic chemical nematicides have triggered an urgent demand for ecologically compatible biocontrol alternatives. Antagonistic microbes and their bioactive metabolites serve as eco-friendly candidates to manage Meloidogyne incognita . This study aimed to screen rhizosphere antagonistic bacteria, characterize their primary nematicidal metabolite, and validate their nematicidal efficacy. RESULTS Strain Pseudomonas nicosulfuronedens MiC45, isolated from the rhizosphere soil of healthy pepper in nematode-infested fields, exhibited potent nematicidal activity, yielding a corrected second-stage juvenile mortality rate of 89.54% at 48 h post-treatment. Liquid chromatography–mass spectrometry (LC–MS) analysis identified azelaic acid as the critical bioactive constituent within its ethyl acetate fermentation extract, with a detected concentration of 505.73 μg/mL. In vitro tests showed that azelaic acid exhibited dose-dependent lethal activity against M. incognita . At 500 μg/mL, the nematodes mortality reached 84.22%, surpassing the positive control abamectin (76.75%). In addition, concentrations ≥ 300 μg/mL greatly suppressed nematode egg hatching, with an inhibition index of 98.71% at 500 μg/mL after 11 days. Moreover, scanning electron microscopy (SEM) and transmission electron microscopy (TEM) imaging revealed cuticle disruption and ultrastructural damage in treated nematodes. Azelaic acid also induced dose-dependent reactive oxygen species (ROS) generation within nematodes. Pot experiments demonstrated that both MiC45 fermentation broth and azelaic acid significantly reduced root gall numbers and nematode invasion, while the fermentation broth simultaneously enhanced pepper biomass. CONCLUSION Azelaic acid from strain MiC45 suppresses M. incognita by destroying body structures and triggering oxidative stress. These findings provide a theoretical foundation for developing novel biopesticides for pepper production and offer a solid basis for environmentally friendly alternatives to conventional chemical nematicides. © 2026 Society of Chemical Industry.
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Foods
·
2023
·Vol. 12
·期 2
·392
·
IF 5.2
Yue Peng,Qian Wang,Kaixiang Zhu,Wu Ding
Pseudomonas aeruginosa(P. aeruginosa) is a typical Gram-negative bacterium that can cause the spoilage of catered food products. Using a luminescent reporter gene (luxCDABE), this study sought to construct a cell-based biosensor (PAO1-CE) to rapidly screen antibacterial substances againstP. aeruginosa. A total of six antibiotics belonging to five categories were used as the model test substances. The results of the bioluminescence detection method were verified using traditional antibacterial research assessments. The correlation coefficient of the regression equation fitting the data generated using this method was greater than 0.98, supporting the credibility of this approach. Additionally, the EC50of each of the antibiotics assessed in this study was lower than the 1/2 MIC determined by conventional means. All six of the antibiotics caused varying degrees of damage to the cell membrane and cell wall ofP. aeruginosa. Importantly, this novel method helped shorten the time necessary for active-compound detection and could be used for high-throughput detection, which would also help improve the detection efficiency. The application of this method towards the discovery of novel antibacterial compounds targetingP. aeruginosaholds substantial promise for greatly improving the efficiency of compound discovery.Keywords:luxCDABE;Pseudomonas aeruginosa;bacteriostatic substances;rapid
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INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
·
2022
·Vol. 76
·期 1
·51-62
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IF 4.286
Huaxiang Li,Yanhe Zhang,Xinhai Yuan,Siqi Liu,Luyao Fan,Xiangfeng Zheng,Shuo Wang,Lei Yuan,Xinan Jiao
This study aimed to reveal microbial populations in Chinese raw milk using both 16S rRNA sequencing and high-throughput sequence (HTS) methods. Psychrotrophic isolates were clustered into 23 genera and 57 species, and the abundant genera in raw milk were Pseudomonas , Acinetobacter and Lactococcus . Genetic diversity and biofilm-forming capacity of Pseudomonas isolates were analysed using random amplified polymorphic DNA (RAPD) and crystal violet staining assay, respectively. Heterogeneous biofilm-forming ability of Pseudomonas isolates with the same RAPD patterns was observed. HTS analysis revealed a 33-fold higher diversity at the genus level, and a different bacterial population was predominated by Flavobacterium , Pseudomonas and Acinetobacter . The results provide insight into the microbial contamination of raw milk.
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危险品化学品经营许可证（带存储）