SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
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2025
·
IF 4.6
Qi Ma,Yueyue Zu,Sanhu Zhao,Hailong Li,Jinping Song,Yongsheng Qiao
Sulfur quantum dots (SQDs) have garnered increasing attention for their versatile applications in bioimaging, LEDs, and fluorescence sensing. As a new type of heavy-metal-free fluorescent nanomaterial, developing high quantum yield SQDs using small molecules as capping agents poses a significant challenge. Here, we present a novel synthesis approach of SQDs using (NH 4 ) 2 S x and sulfur powder as sulfur sources, and the small molecule N-acetyl-L-cysteine (NAC) as a stabilizer in a weak alkaline system. The synthesized blue SQDs (B-SQDs) were extensively characterized using various techniques. B-SQDs exhibited a fluorescence quantum yield of 15.55 % and particle sizes ranging from 2.7 nm to 6.2 nm, showcasing exceptional optical stability, dispersibility, low cytotoxicity, and efficient cell uptake. The as-acquired B-SQDs were explored for potential applications in sensing and cell bioimaging. The findings revealed that B-SQDs exhibited high sensitivity and selectivity towards the drug molecule Rutin. The detection limit was calculated as 50 nM. This methodology was effectively employed for Rutin determination in actual urine samples, yielding satisfactory recovery rates ranging from 95.68 % to 103.93 % with a relative standard deviation below 4.28 %. The fluorescence quenching of B-SQDs by Rutin is influenced by the concentration levels of Rutin. At high Rutin concentrations, fluorescence quenching mechanisms include dynamic quenching and static quenching. Conversely, at low Rutin concentrations, it mainly involves a dynamic quenching mechanism and the IFE process. Moreover, B-SQDs could also accurately identify intracellular Rutin. This research offers a robust approach for Rutin detection in biological samples with implications for disease diagnosis and pharmacology.
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Food Bioscience
·
2025
·
IF 5.9
Shengpeng Zhang,Menglin Jiang,Yao Chen,Genyi Zhang
The water solubility of food bioactives is one of the crucial factors influencing their bioavailability and biological functions. The encapsulation and cellular bioavailability of Rutin, as a representative of bioactive compounds with poor water solubility, was studied. The results showed that Rutin encapsulation by hydroxypropyl-β-cyclodextrin (HPCD) formed an inclusion complex in a molar ratio of 1:1. The encapsulation of Rutin by HPCD exhibited a semi-edged form in which the pyran glucose unit and part of the aromatic ring of Rutin are in the cavity of HPCD through hydrogen bonding and hydrophobic interactions. Rutin encapsulation by HPCD substantially increased the water solubility of Rutin by 51 times. The formed nanoscale inclusion complex (IC) also significantly increased the thermal stability of Rutin with a release kinetics fitting the Korsmeyer-Peppas model. The Rutin encapsulation increased the antioxidant property of Rutin (∼50%) and the inhibitory function on glycolytic enzymes of α-amylase. IC also significantly reduced the postprandial glycemic response to cooked starch (peak glucose level of starch, starch + Rutin, starch + IC are 10.3, 7.2 and 4.7 mmol/L) in mice. Further study using a cell model revealed a significantly increased bioavailability (∼10%). Thus, Rutin encapsulation by HPCD significantly increased its water solubility, bioavailability and biological functions, and high water solubility-induced increase of contact opportunities between Rutin and other components (free radicals, and enzymes) in the reaction system might be the underlying mechanism.
研究领域:
食品科学
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药理学
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生物化学
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JOURNAL OF APPLIED ELECTROCHEMISTRY
·
2026
·Vol. 56
·期 4
·65
·
IF 3.7
Chen Xue,Luo Qiufen,Zhang Hongfang
Developing a simple, rapid and sensitive rutin sensor is of great importance for the quality control or dietary guidance of some agriculture products. Herein, an electrochemical sensor for the quantification of rutin was developed based on the bimetallic nanoparticles with gold as core and platinum as shell. The nanoparticles were prepared by using MoS 2 nanosheets as heterogeneous substrate and reductant for the gold precursor. The morphology of the ultimate nanocomposite was tuned by the amount of gold precursor. With a relatively big amount of chloroauric acid, the nanocomposite exhibited a sea urchin-like core-shell morphology with monodispersity. Moreover, contributing to the large specific area and excellent conductivity of this nanocomposite, the modified electrode prepared with the bimetallic nanoparticles mediated with MoS 2 nanosheets exhibited a response almost twice that of the electrode modified with the single material. As results, the developed electrochemical sensor displayed a broad quantification range from 20 nM to 1 µM with a limit of detection of 3 nM and high sensitivity of 149.3 µA µM − 1 cm − 2 . In addition, the sensor exhibited excellent stability and reproducibility. The recovery results for onion samples demonstrated the application promise of the sensor in various agriculture products. Graphical abstract Adjusting the amount of HAuCl 4 results in two different materials. The performance of electrodes modified with MoS 2 -Au 100 @Pt or MoS 2 -Au 300 @Pt materials is compared. The electrochemical sensor developed by MoS 2 -Au 300 @Pt exhibits the highest sensitivity, and thus is applied to the determination of rutin in onion samples.
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ARCHIVES OF MICROBIOLOGY
·
2026
·Vol. 208
·期 8
·385
·
IF 3.4
Wang Jiahui,Zhu Yuzhang,Liu Tao,Hong Xiangyu,Liu Mi,Zhou Rui,Li Xianke,Jing Mingguo,Yang Lifang
The antibacterial strategy that utilizes ultraviolet-A (UVA)-irradiated natural photosensitizers is promising, being particularly effective against multidrug-resistant pathogens with high bactericidal efficiency and minimal resistance-inducing risk. Rutin is a natural flavonoid with wide availability, low cytotoxicity, and diverse pharmacological activities, making it a promising photosensitizer; however, its UVA-induced photodynamic antibacterial properties remain underexplored. The aim of this study is to systematically evaluate the antibacterial efficacy and mechanism of UVA-Rutin against foodborne Escherichia coli O157:H7 . In the study, Fourier transform infrared spectroscopy and Raman spectroscopy were used to characterize structural alterations in rutin post-UVA irradiation, focusing on functional groups relevant to photodynamic activity. Antibacterial efficacy was assessed via standardized assays including minimum inhibitory concentration (MIC) determination, while biocompatibility was evaluated using MTT cytotoxicity assays. To confirm the antibacterial effects, physiological/structural perturbations of bacteria were measured, including cell membrane integrity, DNA damage, motility, biofilm formation, and extracellular polysaccharide (EPS) production, as well as the generation of intracellular reactive oxygen species (ROS) measured using fluorescent probes. Reverse transcription quantitative polymerase chain reaction was used to analyze key virulence- and quorum sensing-related genes ( luxS , phoP , qseB , qseC ). Results showed that UVA-induced structural changes enhanced the photosensitizing activity of rutin. UVA-Rutin exhibited significant antibacterial activity (low MIC) and good biocompatibility, triggering the generation of massive intracellular ROS, disrupting cell membranes, inducing DNA damage, impairing motility, inhibiting biofilm/EPS production, and downregulating target genes. Collectively, UVA-Rutin exerts potent antibacterial effects via ROS-mediated structural/functional disruption and virulence gene regulation, highlighting its potential as a novel food industry antibacterial agent.
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LUMINESCENCE
·
2020
·Vol. 35
·期 8
·1416-1423
·
IF 1.855
Yunyun Sun,Wei He,Xiangying Sun,Bin Liu
A selective and sensitive method for detecting rutin using MoS 2 quantum dots (QDs) as a fluorescent probe is reported in the paper, a one-step hydrothermal synthesis method was used to prepare MoS 2 QDs. The synthesized MoS 2 QDs had good stability and water solubility, and the fluorescence of MoS 2 QDs was quenched by rutin due to the inner filter effect. There was a good linear relationship between the intensity of MoS 2 QDs and rutin concentration ranging from 5.0 × 10 −7 to 3.2 × 10 −5 mol/L with a detection limit of 3.5 × 10 −7 mol/L. MoS 2 QDs showed temperature-dependent fluorescence that had an excellent linear relationship between 20 and 80°C. Therefore MoS 2 QDs can be used as specific fluorescence sensors for selection of rutin and for temperature sensing.
研究领域:
分析化学
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材料科学
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生物医学工程
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2020
·
IF 3.807
Bowen Zhang,Abdelhadi El Jaouhari,Xiangrong Wu,Wei Liu,Jinhua Zhu,Xiuhua Liu
Rutin is one of the biologically active functional components present in a wide range of pharmacological applications. Herein, we reported the preparation of selective electrochemical sensor based on poly(3,4-ethylene-dioxythiophene) (PEDOT)-mesoporous carbon (MC) for rutin determination. The composite material was synthesized by in-situ polymerization and characterized using different analytical techniques such as FT-IR and SEM to evaluate the composition and the morphology. Cyclic voltammetry was performed for the electrochemical characterization of different modified electrodes and the parameters influencing the sensitivity of PEDOT-MC/AgNPs toward rutin such as accumulation time and pH were optimized. Under optimal conditions, the modified electrode provides a wide linear range and low limit of detection using differential pulse voltammetry (DPV). The GCE/PEDOT-MC/AgNPs electrochemical sensor shows high sensitivity, long duration stability, and good reproducibility and the PEDOT-MC/AgNPs have been successfully used for the detection of rutin in real samples.
研究领域:
材料科学
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分析化学
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药理学
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FOOD CHEMISTRY
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2016
·
IF 4.052
Yao Huang,Fang Feng,Jie Jiang,Ying Qiao,Tao Wu,Josef Voglmeir,Zhi-Gang Chen
In this study, an efficient extraction technique using a combination of ultrasound and natural deep eutectic solvents (NADESs) was developed. Some basic physical properties, including viscosity, polarity, and solubility, of thirteen NADESs prepared from natural components were investigated systematically. Results show that the solubility of rutin increased in choline chloride- and glycerol-based NADESs by 660–1577 times compared to water. NADESs with high rutin extractability can be designed by combining NADESs components. A maximum of 9.5 mg/g rutin was extracted from tartary buckwheat hull with extraction efficiencies of 95%. NADESs can be recovered and recycled. In addition, the biocompatibility and biodegradability of the tested NADESs were also evaluated. The results demonstrated that these NADESs were excellent solvents with extremely low toxicities and favorable biodegradabilities. Our findings suggest that NADESs can be used as green solvents for the extraction of bioactive ingredients.
研究领域:
植物化学
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食品科学
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环境科学
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JOURNAL OF MOLECULAR STRUCTURE
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2022
·
IF 3.841
Jingya Liu,Sidian Zhang,Xiuying Zhao,Yi Lu,Meng Song,Sizhu Wu
Rutin is a natural flavonoid compound with various biological activities, but its low water solubility limits its applications in the food and pharmaceutical fields. Complexation with cyclodextrins (CDs) is a powerful method to improve the poorly solubility of rutin, whereas the mechanism for the inclusion still needs to be explored. This study investigated the characteristic and mechanism for inclusion of rutin with β -cyclodextrin ( β -CD) and 2,6 dimethyl- β -cyclodextrin (DM- β -CD). The molecular simulation provided the possible inclusion structure and revealed that the combination of rutin and CDs is a thermodynamically good process. It also indicated that the complex formed with DM- β -CD had better stability and water solubility compared with β -CD. Then the complexes of rutin with β -CD and DM- β -CD were prepared and characterized by FT-IR, XRD, and DSC analysis. Phase solubility studies demonstrated that the solubility of rutin was significantly enhanced by forming 1:1 stoichiometric inclusion complexes. The effect of DM- β -CD in improving the water solubility of rutin is 146% higher than that of β -CD, which was in accordance with the simulation predictions.
研究领域:
食品科学
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药学
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分子生物学
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SYNTHETIC METALS
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2022
·
IF 4
Jinghao Wu,Shuangshuang Cai,Qiuyu Kong,Meng Chen,Zuanguang Chen
Existing electrochemical sensors generally possess limited sensitivity, which necessitates the use of high-cost materials such as precious metals and enzymes. Therefore, the development of electrochemical sensors that have low synthesis cost, short detection time, simple operating process, high sensitivity and non-precious metals is highly desirable for establishing modern and more efficient electrochemical analysis methods. Herein, we used sodium borohydride reduction method to load Fe and Co onto multi-walled carbon nanotubes (Co,Fe/MWCNT). The successful doping of two metals exposes a large number of active sites in the material and increases the effective area. The morphology and elemental composition of the composite material were determined, and the material was used to modify a glassy carbon electrode for the construction of a hypersensitive electrochemical sensor. Subsequently, the electrochemical behavior of rutin on the constructed sensor was investigated under optimum conditions. The reduction peak current and concentration of rutin were found to exhibit a good linear relationship within the concentration ranges 8 × 10 −4 –8 × 10 −3 µmol/L (R 2 = 0.998), 1 × 10 −2 –0.1 µmol/L (R 2 = 0.996), and 1–200 µmol/L (R 2 = 0.998). The electrode also showed good reproducibility and a low limit of detection of 2.8 × 10 −4 µmol/L (3S 0 /S). Importantly, the sensor was successfully applied for the measurement of rutin content in tablet and urine. Thus, an effective analytical platform for rutin determination during the analysis of real drug and biological samples was developed.
研究领域:
电化学
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材料科学
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分析化学
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药物分析
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2022
·
IF 3.318
Hua Zhang,QianYu Zhao,JunQiang Qiu,ZhanHua Wang,Xin Yang
Rutin is a naturally active compound with biological and medical value. The traditional extraction and separation method not only destroys the structure and activity of rutin, but results in a low extraction rate. In this work, the magnetic micellar molecularly imprinted polymer of rutin with a selective recognition function, i.e., RMMMIP was synthesized from 4 to Vinylphenylboron acid and 4-Vinylpyridine as functional monomer, derivatives of cholic acid as amphiphilic molecules. The internal hydrophobic and external hydrophilic characteristics of micelle was used to weaken the solvation of rutin and strengthen the non-covalent interaction between functional monomer and rutin. Fe 3 O 4 , as the core, endowed the composite materials with good magnetic responsiveness and was easy to separate solid from liquid. Then its structure and adsorption were studied, adsorbing capacity and recognition specific factor of RMMMIP are 11.9 mg·g −1 and 3.55 respectively. RMMMIP was used for the separation of rutin from crude extracts of Sophora japonica Linn and showed a better selective adsorption capacity than quercetin, naringin and cyanidin-3-O-glucose. It indicated that RMMMIP as a specific adsorbent had the potential to be a practical way to purify rutin from rutin crude extracts in the future.
研究领域:
植物化学
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材料科学
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药物化学
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高影响因子
MOLECULES
·
2022
·Vol. 27
·期 19
·6603
·
IF 4.927
Fan Shi,Yijing Ai,Baoli Wang,Yucen Yao,Zejun Zhang,Juan Zhou,Xianghui Wang,Wei Sun
To build a portable and sensitive method for monitoring the concentration of the flavonoid rutin, a new electrochemical sensing procedure was established. By using nitrogen-doped carbonized polymer dots (N-CPDs) anchoring few-layer black phosphorene (N-CPDs@FLBP) 0D-2D heterostructure and gold nanoparticles (AuNPs) as the modifiers, a carbon ionic liquid electrode and a screen-printed electrode (SPE) were used as the substrate electrodes to construct a conventional electrochemical sensor and a portable wireless intelligent electrochemical sensor, respectively. The electrochemical behavior of rutin on the fabricated electrochemical sensors was explored in detail, with the analytical performances investigated. Due to the electroactive groups of rutin, and the specific π-π stacking and cation–π interaction between the nanocomposite with rutin, the electrochemical responses of rutin were greatly enhanced on the AuNPs/N-CPDs@FLBP-modified electrodes. Under the optimal conditions, ultra-sensitive detection of rutin could be realized on AuNPs/N-CPDs@FLBP/SPE with the detection range of 1.0 nmol L−1to 220.0 μmol L−1and the detection limit of 0.33 nmol L−1(S/N = 3). Finally, two kinds of sensors were applied to test the real samples with satisfactory results.Keywords:portable wireless intelligent electrochemical sensor;functionalized black phosphorene nanocomposite;screen-printed electrode;rutin;electrochemistry
研究领域:
分析化学
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材料科学
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药学
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Biosensors-Basel
·
2023
·Vol. 14
·期 1
·18
·
IF 5.4
Yuge Liu,Zhiguo Li,Weizhen Chen,Xiaomiao Feng
In this study, a nanocomposite of LDH/graphene/polyaniline/gold (LDH/rGO/PANI/Au) was synthesized and characterized. The results of characterization showed that the composite material preserved the layered structure of LDH. The composite was dropped onto the glassy carbon electrode and laccase was then immobilized. Electrochemical tests showed that the composite could accelerate the electron transfer between the enzyme and the electrode. The composite/laccase showed an obvious response to rutin and the optimal detection conditions were discussed. The oxidative peak current of the biosensor constructed using the modified electrode was negatively correlated with rutin in the range of 0.05–4 μg/mL. The detection limit was 0.0017 μg/mL at a signal-to-noise ratio of 3. This biosensor of rutin also possessed high sensitivity, excellent anti-interference ability, and stability. The contents of rutin in tablets, first determined using HPLC, were also detected using the sensor constructed in this research as an application, and the results were acceptable. This research here provides a facile way for the fast detection of rutin in real samples.Keywords:rutin;biosensor;LDH/graphene/polyaniline/gold;composite
研究领域:
分析化学
·
生物传感技术
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高影响因子
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