FOOD RESEARCH INTERNATIONAL
·
2021
·
IF 6.475
Chunjun Yan,Zheng Zhou
This study investigated the effects of walnut phenolics and extraction methods on the composition and structural properties of walnut protein isolates (WPIs). Fluorescence quenching experiments showed that walnut phenolics could bind to walnut globulins, albumins, and glutelins with apparent affinity constants of 5.49 × 10 4 M −1 , 1.71 × 10 4 M −1 , and 3.10 × 10 4 M −1 , respectively. However, the UV turbidity and dynamic light scattering (DLS) measurements indicated that phenolics could lead to the severe precipitation of globulins and albumins but not glutelins. The removal of pellicles could significantly increase the yield rate of salt-soluble globulins to approximately 72.8%. Furthermore, salt- and alkaline-extraction methods could produce comparable WPIs yields when using pellicle-free walnut kernels. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and size-exclusive chromatography indicated that the major protein compositions of the salt- and alkaline-extracted WPIs from pellicle-free walnut kernels were similar, while alkaline-extracted WPIs from kernels with pellicles exhibited phenolic-induced protein aggregation. Fourier transform infrared (FTIR) spectroscopy indicated that WPIs produced from kernels with pellicles contained more α-helix and less β-sheet structures than WPIs produced from pellicle-free kernels. These results confirm that walnut pellicle phenolics and the extraction methods could greatly influence the composition and structural properties of WPIs.
研究领域:
食品科学
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化学
·
生物化学
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DOI
PubMed
高影响因子
TALANTA
·
2023
·
IF 6.1
Qing Chen,Yan Xu,Xueting Feng,Yuhan Xiang,Jiayue Ni,Guoyu Ding,Qunxiang Ren,Ming-sheng Zhou
The grafting of a drug molecule, i.e., geldanamycin (GA) onto polyethyleneimine (PEI)-coated magnetic nanoparticle produces a novel composite, GA@Fe 3 O 4 –NH 2 . The composite is confirmed by characterizations with FT-IR, Raman, SEM, EDS, VSM and TEM. Due to the high binding-affinity of GA with myosin heavy chain (MYH), GA@Fe 3 O 4 –NH 2 exhibits excellent adsorption performance towards myosin. Consequently, a solid-phase extraction procedure is established for highly efficient and selective separation of myosin from pig heart extract. At pH 6.0, an adsorption efficiency of 97.1 % is achieved for treating 100 μg mL −1 myosin (0.1 mL) with 0.1 mg GA@Fe 3 O 4 –NH 2 as adsorbent. The adsorption behavior of myosin onto GA@Fe 3 O 4 –NH 2 fits Langmuir model, corresponding to a theoretical adsorption capacity of 518.1 mg g −1 . The adsorbed myosin can be readily recycled by the SDS solution (1 %, m/m) with an elution efficiency of 91.8 %. According to circular dichroism spectroscopy, the conformational changes of myosin during adsorption and elution are reversible. For practical application, myosin is successfully isolated from the pig left ventricular protein extract with GA@Fe 3 O 4 –NH 2 , and SDS-PAGE and LC-MS/MS showed that myosin had high purity and a total of 716 proteins could be identified. Significantly, Geldamycin-encapsulated magnetic nanoparticle for the separation of myosin well exploits the potential of the nanomaterials modified by drug molecules in the separation and purification of target proteins.
研究领域:
蛋白质组学
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纳米技术
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生物医学工程
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分析化学
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DOI
PubMed
高影响因子
JOURNAL OF FOOD SCIENCE
·
2024
·Vol. 89
·期 12
·9257-9270
·
IF 3.2
Guangliang Xing,Jia Liu,Ruohan Wang,Yitong Wu
Transglutaminase (TGase)-mediated cross-linking has gained significant attention due to its potential to reduce the allergenicity of food proteins. This study investigates the effects of TGase cross-linking on allergenicity and conformational modifications in a dual-protein system comprising soy protein isolate (SPI) and β-lactoglobulin (β-LG). The results showed that TGase cross-linking effectively decreased the allergenic potential of both SPI and β-LG, with a more pronounced reduction observed in the allergenicity of soy protein in the dual-protein system. SDS-PAGE analysis revealed that the 7S and 11S subunits of soy protein were more easily cross-linked than β-LG. Secondary structure analysis indicated that TGase treatment disrupted β-sheet structures, increased the content of random coils, and enhanced protein flexibility. Ultraviolet absorption and intrinsic fluorescence analyses confirmed these structural alterations, with TGase treatment exposing additional aromatic amino acids. A reduction in free sulfhydryl groups and altered intermolecular forces further corroborated the occurrence of cross-linking. These findings suggest that TGase-mediated cross-linking effectively reduced the allergenicity of SPI and β-LG by modifying their conformations, offering potential strategies for the development of hypoallergenic dual-protein food products. Practical Application This study has practical applications in the food industry to develop hypoallergenic food products, particularly those that combine soy and dairy proteins. By using TGase to cross-link these proteins, the allergenicity can be reduced, resulting in products that are safer for consumers with food allergies.
研究领域:
食品科学
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过敏研究
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蛋白质化学
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生物技术
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产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Wanxing Zheng,Yufei Tang,Rui Yang,Zhangwen Xie,Yani Sun,Kang Zhao
Effectively attenuating highly penetrative low-frequency noise (LFN, f < 500 Hz) presents a significant challenge. Traditional acoustic insulation materials are circumscribed by the mass law, presenting an inherent conflict between lightweight design and acoustic insulation. Despite the promising potential of PUA aerogels in acoustic insulation, attributed to their lightweight and high damping properties, their low-density results in inadequate areal density. Consequently, it remains challenging to effectively suppress LFN through mass inertia alone. Moreover, lightweight design often reduces mechanical properties, creating a conflict between lightweight, high strength, and low-frequency acoustic insulation. Inspired by the periodic structures of metamaterials, this study fabricates a PUA aerogel with an ordered metamaterial-like structure (density: 0.126 g/cm 3 ). Through self-assembly induced by sodium dodecyl sulfate (SDS), an ordered porous cavity and fibrous topological skeleton were formed. This structure creates an efficient interfacial reflection network that provides dynamic acoustic impedance for LFN attenuation. The average STL is at least 42.6 dB in the 45–500 Hz range and 39.6 dB in the broader 45–6300 Hz range, 16.35 times higher than that of commercial melamine foam of the same 2 cm thickness. Its weight is just 1/770 that of lightweight concrete bricks with equivalent noise reduction performance, and its thickness is only 2/25 of theirs. At 145 °C, the aerogel maintains a temperature difference of approximately 105.05 °C between its surfaces. The compressive strength of the modified aerogels is 2.76 times that of unmodified PUA aerogels. The simple, cost-effective fabrication process and the lightweight, high-strength, and outstanding low-frequency acoustic insulation properties make metamaterial-like PUA aerogels promising for noise reduction in residential, transportation, and industrial applications.
研究领域:
材料科学
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声学超材料
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噪声控制工程
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聚合物纳米复合材料
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可持续建筑材料
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 12
·1-10
·
IF 5.705
Wang Meng-Meng,Chen Shuai,Zhang Dan-Dan,Yu Yong-Liang,Wang Jian-Hua
A sorbent for selective extraction of phosphoproteins was obtained by immobilization of a Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide (CeEGO). The resulting composites exhibit an adsorption capacity of 981 mg g −1 for β-casein due to the synergistic effect of metal-affinity interaction between Ce(IV) and phosphate groups and π-stacking interaction between the polyoxometalate framework and the phosphate groups. The results of LC-MS and SDS-PAGE analysis show that the CeEGO composites can be applied to the extraction of phosphoproteins from protein mixture, and as little as 50 μg mL −1 of the phosphoprotein β-casein can be detected by SDS-PAGE. It was also applied to the extraction of β-casein from spiked biological samples such as drinking milk, whole blood and swine heart tissue extract.
An efficient sorbent is obtained by immobilization of a Ce(IV)-substituted polyoxometalate on ethylenediamine-functionalized graphene oxide (CeEGO). The resulting composites exhibit highly selective capture capacity towards phosphoproteins due to the synergistic effect of metal-affinity interaction between Ce(IV) and phosphate groups and π-stacking interaction between the polyoxometalate framework and the phosphate groups.
研究领域:
分析化学
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生物化学
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材料科学
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产品关联
DOI
PubMed
高影响因子
FUEL
·
2017
·
IF 4.601
Shiwei Wang,Longfei Tang,Xiuxiang Tao
In this paper, effect of surfactants on the hydrophobicity of low rank coal particles by sliding time measurements was investigated. X-ray photoelectron spectrometer (XPS) analysis indicated that the surfaces of low rank coal particle consisted of many hydrophilic functional groups. It was observed that the sliding time of low rank coal particles in 2-ethyl hexanol solution was the shortest than that of low rank coal particles in dodecyl amine hydrochloride (DAH) and sodium dodecyle sulfate (SDS) solutions. Moreover, the maximum touching angle (θ T ) of low rank coal particles observed in 10 −2 mol/L of 2-ethyl hexanol solution was about 80°, which was the greatest than that of low rank coal particles, about 39° in 10 −3 mol/L of DAH and 35° in 10 −4 mol/L of SDS solutions, respectively. Therefore, the sliding time results indicated that the hydrophobicity of low rank coal was enhanced by 2-ethyl hexanol and DAH and reduced by SDS. However, it was noted that the hydrophobicity of low rank coal was depressed when the DAH concentration increased to 10 −2 mol/L. The flotation performances were consistent with the results of sliding time measurements. Therefore, effect of surfactants on the hydrophobicity of low rank coal particles can be characterized by sliding time measurements.
研究领域:
化学工程
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材料科学
·
环境科学
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产品关联
DOI
高影响因子
Journal of Chemistry
·
2023
·
IF 3
Dawei Zhang,Zhenhua Zhou,Guifeng Sun,Shiying Chen,Minzhi Jiang,Zihan Yao,Anthony Chukwunonso Ogbuehi,Hao Wang,Lei Jin
Protein glycosylation and phosphorylation are two important post-translational modifications and play significant roles in various biological processes. However, both glycoproteins and phosphoproteins are in low abundance in complex samples, which hinders their characterization. In this work, we developed a novel method for the simultaneous adsorption of glycoproteins and phosphoproteins based on solid phase extraction. By virtue of the unique structure of hydrophilic titanium (IV) ion immobilized cotton fiber functionalized with phytic acid (denoted as Ti4+-PA-cotton), glycoproteins, and phosphoproteins could be efficiently enriched in one-step incubation and eluted in sequence. The newly prepared Ti4+-PA-cotton was well characterized, and the adsorption isotherms and kinetics were studied. The maximum adsorption capacities of Ti4+-PA-cotton for β-casein and HRP were 833.3 mg/g and 384.6 mg/g with the adsorption rate of 259.1 and 63.1 mg/g·min. Standard protein mixture of BSA, HRP, and β-casein was used to test the enrichment ability as a proof of concept. The SDS-PAGE results demonstrated that Ti4+-PA-cotton had an excellent enrichment performance and possessed an outstanding application potential in the analysis of glycoproteins and phosphoproteins.
研究领域:
生物化学
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分析化学
·
蛋白质组学
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产品关联
DOI
高影响因子
Fishes
·
2024
·Vol. 9
·期 12
·512
·
IF 2.1
Lili Pu,Shuting Dai,Zongming Wu,Sufang Wang,Yongbo Bao
This study investigates the essential role of the heme cavity in the peroxidase and antibacterial activities of homodimeric hemoglobin (Tg-HbI) from the blood clamTegillarca granosa. After treatment with sodium dodecyl sulfate (SDS), the peroxidase and antibacterial activities of the Tg-HbI were significantly inhibited, with the degree of inhibition correlating positively with the SDS concentration. Fluorescence spectroscopy, UV-Vis spectroscopy, and molecular docking analysis further revealed that SDS interacts with key amino acid residues (e.g., His70 and His102) in the heme cavity of Tg-HbI, causing conformational changes that disrupt the internal hydrophobic interactions, thus inhibiting its function. This study confirms that the antibacterial effect of Tg-HbI is mediated through its peroxidase activity and that the heme cavity plays a critical role in maintaining this activity. These findings lay a foundation for further research on the immune defense functions of hemoglobin and provide new insights into the mechanisms of environmental adaptation inT. granosa.
研究领域:
生物化学
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免疫学
·
海洋生物学
·
分子生物学
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产品关联
DOI
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
·
2017
·
IF 4.9
Rui Zhang,Yang Liu,Xinran Huang,Mengchen Xu,Rutao Liu,Wansong Zong
The extensive use of surfactants in food, laundry products and agriculture has caused concern about their biosafety. However, few studies have been done on their potential effect on the lipase which has always been used with surfactants in food and laundry industry. Herein, we investigated the interaction of three surfactants (sodium dodecyl sulfate (SDS), sodium dodecyl benzene sulfonate (SDBS), sodium lauryl sulfonate (SLS)) with Candida rugosa lipase (CRL), which is a popular biocatalyst used regularly with surfactants. The effect of the three surfactants on the conformation and activity of CRL was evaluated by using multiple spectral methods, enzyme activity assay and molecular docking modeling. The results demonstrated that CRL interacted with SDS, SDBS and SLS primarily through hydrophobic forces, H-bonding and electrostatic forces, respectively. The binding constants (K A ) of SDBS with CRL varied with temperature: 1.99 × 10 3 mol/L at 298 K and 4.13 × 10 3 mol/L at 318 K. SDS and SDBS affected the secondary structure and skeleton of CRL, which changed the polarity of CRL and enhanced its activity. SLS also changed the secondary structure and activity of CRL moderately, but had little effect on its polarity and chromophore microenvironment. Accordingly, all three surfactants exhibited effect to CRL on the molecular level calling for more attention to pay on their biosafety. The work demonstrates that SDS, SDBS and SLS could cause negative effects to CRL from different angles and therefore are not bio-friendly detergents.
研究领域:
生物化学
·
分子生物学
·
酶学
·
环境科学
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产品关联
DOI
PubMed
高影响因子
FUEL
·
2022
·
IF 8.035
Qiming Zhuo,Wenli Liu,Penghui Wang,Jiushuai Deng,Peng Xi,Yuzhe Hua
The precise separation of macerals is the premise and foundation for the efficient utilization of coal resources, and flotation is the separation method with the greatest industrial potential. The differences in the surface hydrophobicity of macerals provide the basis for flotation, but the complexity of their surface properties makes separation challenging. In this study, sub-bituminous coal was used to obtain vitrinite and inertinite , and a detailed analysis was carried out on the samples. The surface morphologies of vitrinite and inertinite were observed in the QI TM (quantitative imaging) mode of AFM (atomic force microscope) to explore the adsorption of the reagents. The effect of the reagents on the interaction forces between the coal particles and bubbles was measured to reveal the change in hydrophobicity. The attachment performance of macerals under different reagent treatments was investigated using a laboratory-built apparatus. Overall, the results demonstrated that the difference in zeta potential between vitrinite and inertinite was a viable approach for separating the macerals. CTAB adsorption on coal samples changed the surface morphology and the interaction force between particles and bubbles. Interestingly, CTAB adsorption on the gas–liquid interface decreased the interaction force. Vitrinite and inertinite attachment performances were enhanced when Al 3+ and SDS were used simultaneously.
研究领域:
矿物加工
·
表面化学
·
煤炭科学
·
材料科学
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产品关联
DOI
高影响因子
JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Hui Liu,Mengya Li,Keyong Tang,Jie Liu,Xiumin Li,Xueping Meng
Conformations of secondary and super secondary structure from bovine hides collagen are very important for constructing collagen molecular space structure, and directly related to its properties. In this work, the evolutional mechanism of the collagen conformation in the environment of sodium sulphide (Na 2 S) solution is periodically studied by FT-IR spectroscopy, the Gaussian peak fitting of secondary derivative, and auxiliary methods such as SDS-PAGE, SEM and TG. As a result, the three polypeptide chains structure of bovine hide collagen was hardly affected by the ions of OH − , HS − and S 2− . The conformation of collagen molecules such as β-turn, triple helix and β-strand were gradually evolved into unordered structure and α-helix with the sequential addition of OH − , HS − and S 2− , which is because the hydrogen bond inside collagen molecular is extended or opened with the increase of the synergistic effect of pH and reducibility of HS − and S 2− in the solution. The bound water content and thermal properties of the treated collagen molecules are decreased. Finally, the order of the hydrolysis ability to collagen is NaOH < NaHS < Na 2 S at the same condition, which was confirmed by measuring the content of hydroxyproline from their solutions after treating the collagen. This work provides very important information for understanding the evolutional mechanism of bovine hide collagen conformation in the Na 2 S solution, developing the alternatives of sulfide, extraction of collagen, tanning, and application of collagen in biological and material science.
研究领域:
材料科学
·
生物化学
·
胶原蛋白研究
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鞣制技术
·
生化研究
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产品关联
DOI
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2023
·
IF 5.2
Dongxiang Li,Xing Qian,Changshuo Lian,Rui Huang,Ziyi Hao,Ming Cheng,Shuping Li,Chunfang Li,Shahid Iqbal,Lianjie Qin,Jikuan Zhao
The critical micelle concentration (CMC) is one main parameter of surfactants , so an accurate, rapid, and convenient method for CMC determination is of great significance for surfactant applications. In this paper, three samples of carbon quantum dots (CQDs) with different fluorescence properties were developed as fluorescent probes for the CMC determination of CTAB , SDS , Triton X-100, perfluoro OBS and Pluronics® P-123. The fluorescence excitation and emission spectra of a constant amount of the CQDs in different concentrations of the surfactant solution were recorded, and then the CMC values of the surfactant were deduced from the fluorescence intensity change and the Stokes shift variation. The obtained CMC data using three samples of the CQDs agree well with those in the published literature. It is also demonstrated that in the micellization process the interaction between the CQDs and the surfactant molecules is complicated, because the fluorescence properties of the CQDs are found to be related to the characteristics of the CQDs, the head group type of ionic surfactants , the chemical structure between the hydrophobic and hydrophilic segment, and the chemical nature of the hydrophobic chains of the surfactants. So, the CQDs are demonstrated to be located at the palisade layer in the surfactant micelles due to the surface-attached polar groups on the CQDs although the CQDs are mainly hydrophobic as fluorescent probes. The fluorescent CQDs are considered to be new choices of the fluorescent probes in the facile CMC measurement of the surfactants.
研究领域:
化学
·
材料科学
·
分析化学
·
胶体科学
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产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）