ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
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2022
·
IF 7.129
Ruifen Kang,Sheng Li,Aneela Perveen,Jiakun Shen,Chunmei Li
T-2 toxin belongs to the trichothecenes group A compound, mainly produced by Fusarium fungi. It has been shown that T-2 toxin could cross the placental barrier and breast milk, thus endangering the health of offspring. The present study aimed to explore the effects of maternal T-2 toxin exposure on the integrity of the intestinal barrier and the intestinal microflora of young mice. From late pregnancy (GD 14) to lactation (LD 21), pregnant mice were given T-2 toxin daily at 0, 0.005, or 0.05 mg T-2 toxin/kg BW. Postnatal day 21 (PND21), PND28, and PND56 young mice were chosen as objects to detect the influences of maternal T-2 toxin exposure to mice on the offspring. The results showed that maternal exposure to T-2 toxin disturbed the balance of the intestinal microbial flora of the young mice. Villous adhesions and fusion of ileum were observed in T-2-treated groups. In addition, supplementation of T-2 toxin significantly decreased the gene expressions of Claudin 1 , Occludin , Tjp1 , Il10 , Il6, and Tnf in PND 21. However, in PND 28, the expressions of Tnf were significantly increased . T he expressions of Claudin 1 , Occludin , Tjp1 , Il10 , Il6 and Tnf were significantly increased after T-2 toxin treatment in PND 56. These results suggested that maternal exposure to T-2 toxin has negative influences on the intestine of young mice, which may be due to the alterations of microbial composition.
研究领域:
毒理学
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微生物学
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发育生物学
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免疫学
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DOI
PubMed
高影响因子
FOOD CHEMISTRY
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2025
·
IF 9.8
Xufang Huang,Jing Wu,Xiaorui Xing,Yi Wang,Changzheng Wu,Shijie Li,Shuo Wang
Heavy metals and mycotoxins are important contaminants in food pollution. Sensitive, reliable, and rapid detection of heavy metals and mycotoxins is crucial for human health. In this work, imidazole-functionalized aggregation-induced emission (AIE) molecule tetra-(4-pyridylphenyl) ethylene (TPPE) was used as a precise and specific probe for Ag + detection, with a limit of detection (LOD) of 0.0318 μM. Meanwhile, a large amount of hydrophobic TPPE molecules were loaded into the amphiphilic block copolymer F127 to form ultrabright fluorescent microspheres (TPPENPs). Lateral flow immunochromatography (LFIA) based on immunoprobe (TPPENPs-Ab) has been successfully developed. The LOD of TPPENPs-LFIA for T-2 toxin was 0.13 μg/L, which was 13.31-fold and 8.62-fold more sensitive than that of gold nanoparticles-based LFIA and ordinary fluorescent microspheres-based LFIA, respectively. TPPENPs-LFIA was employed to detect T-2 toxin in actual grain samples, with a spiked recovery rate of 71.69 - 111.13 %. This assay offers a promising strategy and new idea for multi-target detection.
研究领域:
食品科学
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分析化学
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生物医学
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DOI
PubMed
高影响因子
LANGMUIR
·
2024
·
IF 3.7
Qianrui Xu,Zhimin Zhao,Xinyu Chen,Wenqian Fan,Yong Jiang
Fe3O4 nanoparticles occupy a pivotal position in the realm of nanobiology due to their nontoxic, biocompatible, and superparamagnetic properties. This study examines the influence of surface modifiers on the properties of magnetic nanoparticles. Poly(methacrylic acid) (PMAA), poly(4-styrenesulfonic acid-co-maleic acid) sodium salt (PSSM), trisodium citrate (TSC), carboxymethylcellulose (CMC), and carboxymethylated-dextran 40 (CMD40) were introduced into a one-pot solvothermal method to synthesize magnetic nanoparticles. TEM, the 4-(bromomethyl)-6,7-dimethoxy coumarin (BMMC) absorption assay, and the Bradford method were employed to characterize the diameter, carboxyl content, and protein immobilization ability of the nanoparticles, respectively. The findings revealed that CMD40-modified magnetic nanoparticles (CMD40-MNPs) exhibited the highest carboxyl content and streptavidin (SA) immobilization content, reaching 6.5 × 10–7 mol/mg and 375 μg/mg, respectively. In contrast, CMC-modified magnetic nanoparticles displayed opposite trends. This is primarily attributed to dextran’s unique molecular structure, which enhances its water solubility and biocompatibility, thereby facilitating contact with Fe3O4 nanoparticles in aqueous solutions. CMD40-MNPs possess a saturation magnetization value of 60.90 emu/g and can be collected within (60 ± 5) s using a standard magnetic separator. Cytotoxicity assays demonstrated that CMD40-MNPs are nontoxic to cells. A cell sorting strategy utilizing the binding of SA-CMD40-MNPs and biotin antihuman CD3 antibody-modified cell suspensions was employed to isolate CD3+T cells. The results indicate that the purity and efficiency of targeted CD3+T cells are 85.2% and 61.5%, respectively.
研究领域:
纳米生物学
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生物医学工程
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材料科学
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DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
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2022
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IF 9.221
Xiya Zhang,Mingyue Ding,Yexuan Mao,Xianqing Huang,Xinhua Xie,Lianjun Song,Mingwu Qiao,Jianwei Zhang,Tianlin Wang,Haihua Zhu,Zizhe Li,Youyi Wang,Meng Dang
The “turn-off” mode lateral flow immunoassay (LFA) based on the conventional competitive format and “turn-on” mode LFA mainly determined by inner filter effect (IFE-LFA) were objectively compared using T-2 toxin as a model molecule and T-2-BSA and anti-T-2 monoclonal antibody as immunocomplex pairs to improve the sensitivity of the LFA. First, Au nanoparticles (Au NPs), amorphous carbon nanoparticles (ACNPs), quantum dots nanospheres (QDs), and time-resolved fluorescent microspheres (TRFMs) were selected as labels for developing the “turn-off” mode LFAs (Au NPs-LFA, ACNPs-LFA, QDs-LFA, and TRFMs-LFA, respectively). Thereafter, Au NPs and ACNPs were used as absorbers, and QDs and TRFMs were selected as fluorescers, for preparing the “turn-on” mode IFE-LFAs (IFE-LFA based on Au NPs as absorbers and QDs as fluorescers [Au NPs-mAb-QDs-BSA], IFE-LFA based on Au NPs as absorbers and TRFMs as fluorescers [Au NPs-mAb-TRFMs-BSA], IFE-LFA based on ACNPs as absorbers and QDs as fluorescers [ACNPs-mAb-QDs-BSA], and IFE-LFA based on ACNPs as absorbers and TRFMs as fluorescers [ACNPs-mAb-TRFMs-BSA]). Under optimized conditions, the naked-eye observation cut-off values for the aforementioned eight LFAs were 4, 2, 2, 2, 3, 2, 1.5, and 1 ng/mL, with quantitative limit of detection values of 0.50, 0.23, 0.24, 0.23, 0.47, 0.45, 0.27, and 0.22 ng/mL, respectively. Among all LFAs, ACNPs-mAb-TRFMs-BSA showed the highest sensitivity. The results of this study provide a perspective for improving the sensitivity of the LFAs to meet the requirements of on-site screening for trace substances.
研究领域:
生物化学
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分析化学
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食品科学
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DOI
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
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2025
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IF 9
Yuan Li,Yang Zhou,Yuze Zhang,Xiaoqing Wang,Jinping Li,Jiangfeng Yang
Although zeolite adsorbents are widely used for gas separation, current studies predominantly focus on single-phase topological structure. Herein, an intergrowth T zeolite with ERI and OFF topologies was synthesized in a template-free system, and the results showed the CH 4 adsorption capacity and CH 4 /N 2 selectivity were 34.4 cm 3 /g and 5.3 (298 K, 1 bar), respectively. The exceptional performance was attributed to its high microporosity and the 6-member ring (6-MR) structure at the intergrowth interface, which synergistically optimize the distribution of adsorption sites, as evidenced by density functional theory (DFT) calculations. Breakthrough experiments demonstrated that intergrowth T zeolite can effectively separate CH 4 /N 2 mixtures (50/50 and 20/80; v/v) with actual separation times of 7.0 min and 10.4 min. Furthermore, two-bed six-step pressure swing adsorption (PSA) simulations further indicated that 20 % of the pristine CH 4 could be enriched to over 50 %, with a recovery rate of 87 %, demonstrating significant potential for upgrading low-concentration coal-bed methane.
研究领域:
化学工程
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材料科学
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物理化学
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分离技术
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能源工程
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产品关联
DOI
高影响因子
Animals
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2019
·Vol. 10
·期 1
·74
·
IF 1.832
Jiakun Shen,Aneela Perveen,Niaz Kaka,Zhaojian Li,Pengyuan Dai,Chunmei Li
Simple SummaryThis study investigated the effects of maternal T-2 toxin exposure on the development of testis in the mice offspring. The detrimental effects were assessed by testicular weight, antioxidant capacity, and testosterone synthesis and secretion. Studies have shown that the toxin carried by the mother has bad effects on the testicular development of offspring at puberty, affecting the antioxidant system and testosterone synthesis in the testis, but the maternal exposure of T-2 toxin had no significant impact on the testes of offspring after sexual maturity, suggesting the recovery of reproductive function.AbstractT-2 toxin, the most toxic member of trichothecene mycotoxin, is widely distributed in cereals, and has been extensively studied, but few studies focus on the toxicity of maternal exposure to offspring. This study focused on the effects of maternal exposure to T-2 toxin (during gestation and lactation) on the testicular development of mice offspring. Dams were orally administered with T-2 toxin at 0, 0.005, or 0.05 mg/kg body weight from the late stage of gestation to the end of lactation. Testicular samples of the mice offspring were collected on the postnatal day 21, 28, and 56. The results showed significant decreases in body weight and testicular weight on the postnatal day 28. Moreover, significant inhibition of antioxidant system and testosterone synthesis was detected on the postnatal day 28. Furthermore, there were significant decreases in the gene expression levels ofStARand3β-HSD, which are involved in testosterone synthesis. In general, present results demonstrated that maternal exposure to T-2 toxin during gestation and lactation led to bad effects on the capacity of antioxidant system and inhibited testosterone synthesis in testes during pre-puberty with no significant effects on post-puberty.Keywords:maternal exposure;oxidative stress;T-2 toxin;testicular development;testosterone synthesis
研究领域:
毒理学
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生殖生物学
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内分泌学
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DOI
PubMed
高影响因子
Nano Today
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2024
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IF 13.2
Jingyi Liu,Qing You,Siyuan Ye,JinJin Wang,Rong Du,Fuming Liang,Zhaohui He,Chen Wang,Ling Zhu,Yanlian Yang
Photothermal therapy (PTT) can mediate the immunogenic cell death (ICD) of tumors through photothermal transformation, thus releasing tumor-associated antigens (TAAs) and damage-associated molecular patterns (DAMPs) to stimulate the maturation of dendritic cells (DCs) and elicit the immune response against tumors. However, PTT-mediated upregulation of the immune checkpoint molecule programmed cell death ligand 1 (PD-L1) will cause the immune evasion of tumor cells and thus increases the immunosuppression of cytotoxic T lymphocytes (CTLs), resulting in restricted photothermal immune activation. Taking advantage of the high expression of programmed cell death protein 1 (PD-1) in T lymphocytes, we develop an immunogenic photothermal hybrid nanovesicle to reverse immunosuppression and enhance PTT-induced ICD. The hybrid vesicle (TMVL-I) is constructed by phospholipid bilayer fusion of T cell-derived membrane vesicle (TMV) with high PD-1 expression and the indocyanine green (ICG)-loaded thermosensitive liposome (L-I). TMVL-Is bind to the tumor cells with PTT-induced upregulation of PD-L1, effectively blocking the PD-1/PD-L1 pathway between CTLs and tumor cells, thus reversing PD-L1-mediated tumor immunosuppression and intensifying photothermal-induced ICD effects. These processes significantly induce the maturation of DCs and enhance the infiltration of CTLs into both primary and distal tumors and the activation of spleen CTLs, strongly inhibiting the growth of B16 xenograft tumors and greatly improving the survival rate of B16 xenograft mice. This work provides a feasible strategy for immunosuppressive microenvironment reprogramming-based combination therapy.
研究领域:
癌症治疗
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纳米技术
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免疫学
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生物医学工程
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产品关联
DOI
高影响因子
Clinical and Vaccine Immunology
·
2005
·Vol. 12
·期 11
·
IF 0
Yongjun Jiang,Hong Shang,Zining Zhang,Yingying Diao,Di Dai,Wenqing Geng,Min Zhang,Xiaoxu Han,Yanan Wang,Jing Liu
Natural killer (NK) cells, natural killer T (NKT) cells, and T lymphocytes were analyzed by using a flow cytometer in 225 human immunodeficiency virus (HIV)-positive individuals infected through the past sale of blood and plasma without receiving antiretroviral therapy in the People’s Republic of China. According to CD4 T-cell counts these HIV-infected adults were stratified into three groups: long-term slow progressors, HIV-infected subjects, and AIDS patients. NK cell counts in long-term slow progressors were higher compared to HIV infection and AIDS patients (P < 0.05) and lower compared to normal controls (P < 0.05), whereas NKT cell counts in slow progressors and the HIV infection group were not different from those of normal controls. NK cell counts in HIV-seropositive subjects were positively correlated with CD4 T-cell counts (P < 0.05), and NKT cell counts were positively correlated with CD4 T-cell and CD8 T-cell counts (P < 0.05). The CD8 T-cell counts were higher in slow progressors compared to those with HIV infection, AIDS patients, and normal controls. These results indicated that HIV infection causes alterations of NK cells and T cells in slow progressors, HIV-infected subjects, and AIDS patient groups, but no difference was found in NKT cell counts and percentages in slow progressors and the HIV-infected group compared to normal controls.
研究领域:
免疫学
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病毒学
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DOI
PubMed
BIOSENSORS & BIOELECTRONICS
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2022
·
IF 12.545
Yan Zhao,Changtong Lu,Xian-En Zhao,Weiheng Kong,Shuyun Zhu,Fengli Qu
DNA-templated silver nanoclusters (DNA/AgNCs) serve as a useful electrochemical sensing nanomaterial characterized by excellent electroactivity and good stability, while the effect of surrounding nucleotides on their electroactivity has not been studied. Herein, we validated a nucleotide-assisted enhancement mechanism of the DNA/AgNCs electroactivity caused by T-rich nucleic acid sequences in the vicinity of AgNCs. Based on the T-rich nucleic acid-enhanced AgNCs (NAE-AgNCs) combined with hybrid chain reaction (HCR), a novel signal-enhanced electrochemical biosensing platform was established for the ultrasensitive detection of miRNA . In the presence of target miRNA-155, HCR could be triggered to generate duplex strands containing both numerous AgNC synthesis sites and T-rich overhang strands upon the electrode. With the electrodeposition of adjacent AgNCs on the electrode, the larger oxidation potential of T-rich nucleic acid leaded to stronger electron-accepting capacity, which could contribute to increased current responses. The T-rich NAE HCR electrochemical strategy resulted in a detection limit of 0.39 fM for miRNA-155 detection, one order of magnitude lower than conventional HCR-based electrochemical sensors . This T-rich nucleic acid-assisted enhancement mechanism provided a new direction to construct highly sensitive, label-free, low-cost, and simple sensing platforms for applications in biomarker assays and clinic diagnosis.
研究领域:
纳米技术
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电化学
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生物传感器
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分子生物学
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DOI
PubMed
高影响因子
JOURNAL OF ORAL PATHOLOGY & MEDICINE
·
2021
·Vol. 51
·期 1
·74-85
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IF 4.253
Jing-Ya Yang,Fang Wang,Gang Zhou
Background Oral lichen planus (OLP) is a T-cell-mediated chronic inflammatory disease with uncertain aetiology. Mucosal-associated invariant T (MAIT) cells and γδT cells are unconventional, innate-like T cells with immunoregulatory capacity. This study aimed to investigate the potential effects of MAIT and γδT cells on the pathogenesis of OLP. Methods Circulating MAIT cells and γδT cells were identified using flow cytometry. Surface proteins including CD4, CD8, CD69, CD103, CD49d, programmed death-1 (PD-1) and its ligand PD-L1 were assessed. Cytokines containing interleukin (IL)-4, IL-17, interferon (IFN)-γ, granzyme B and tumour necrosis factor (TNF)-α released by MAIT and γδT cells were measured following PMA and ionomycin stimulation. Results Circulating MAIT and γδT cells were deficient in OLP. The percentage of CD4 + , CD69 + , CD103 + and PD-1 + MAIT cells was increased in OLP, while that of CD8 + and CD49d + MAIT cells was decreased. The percentage of CD103 + , PD-1 + and PD-L1 + γδT cells was upregulated in OLP. Both the MAIT and γδT cells in OLP produced less IL-4 than controls. The granzyme B-producing MAIT cells were increased, while γδT cells secreting granzyme B and TNF-α were reduced in OLP. IL-17 and IFN-γ in OLP MAIT and γδT cells were not significantly different from that in controls. The frequency of OLP MAIT cells and the MAIT/γδT rate were positively associated with the disease severity. Conclusion The deficient MAIT and γδT cells expressing functional proteins and releasing cytokines may play an immunoregulatory role in the pathogenesis of OLP.
研究领域:
免疫学
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炎症
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口腔疾病
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细胞生物学
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DOI
PubMed
高影响因子
BIOMATERIALS
·
2024
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IF 12.8
Guoquan Wu,Tianyu Su,Peng Zhou,Rongze Tang,Xu Zhu,Jin Wang,Minghao Chao,Liying Fan,Hanrong Yan,Peng Ye,Dehong Yu,Fenglei Gao,Hongliang Chen
Nanomedicines for immune modulation have made advancements in the treatment of rheumatoid arthritis (RA). However, due to aberrations in patients' immune systems, inducing antigen-specific immune tolerance while halting disease progression remains a significant challenge. Here, we develop a highly targeted multifunctional nanocomplex, termed M2Exo@CuS-CitP-Rapa (M2CPR), with the aim of selectively inhibiting inflammatory immune reactions while promoting immune tolerance towards specific antigens. M2CPR specifically targets inflammatory tissues in RA, delivering CuS NPs, CitP, Rapa, and endogenous anti-inflammatory factors, thereby ameliorating the inflammatory joint microenvironment. CuS NPs induce Cuproptosis of activated T cells, whose fragments are engulfed by resident or recruited macrophages, resulting in abundant production of TGF-β. TGF-β acts synergistically with Rapa to induce the iDCs into tDCs. tDCs present CitP to Naive T cells, promoting Tregs differentiation. Tregs, in turn, produce more TGF-β, inducing tDCs differentiation, thereby establishing a cycle of immune tolerance. Through in vitro and in vivo experiments, we validate that M2CPR can induce robust and durable antigen-specific immune tolerance, offering a new paradigm for RA therapy.
研究领域:
免疫学
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纳米技术
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风湿病学
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药学
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产品关联
DOI
PubMed
高影响因子
Small
·
2024
·Vol. 20
·期 51
·2407038
·
IF 13
Xuefan Xiong,Ying Zhang,Xinqi Huang,Shusheng Zhang,Qiong Li
Photothermal therapy (PTT) against cancer not only directly ablates tumors but also induces tumor immunogenic cell death (ICD). However, the antitumor immune response elicited by ICD is insufficient to prevent relapse and metastasis because of the immunosuppressive tumor microenvironment (TME). A biomimetic nanoplatform (bmNP) mimicking cytotoxic lymphocytes (CTLs) for combinational photothermal-immunotherapy to effectively regulate the immunosuppressive TME is reported here. The bmNP is constructed by wrapping the T -cell membrane onto a new type of photothermal agents, spherical Au-based PNCs (sAuPNCs). Similar to T -cells, the bmNP enhanced accumulation at the tumor site by targeting the tumor via adhesion proteins on T -cell membrane. The obtained sAuPNCs have a wide absorption band in the second near-infrared (NIR-II) region with a high photothermal conversion efficiency (PCE) up to about 75% and excellent photostability. The bmNP with a smaller size is more superior compete with T -cells to bond with tumor cells via PD-1/PD-L1 interaction to effectively block the PD-1 checkpoint of T -cells for preventing T -cell exhaustion. Furthermore, in vivo studies reveal the immunological memory effect is significantly elicited in mice received bmNPs therapy. Collectively, bmNPs show great potential in photothermal-enhanced immunotherapy.
研究领域:
肿瘤学
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免疫学
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纳米医学
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）