MICROCHIMICA ACTA
·
2022
·Vol. 189
·期 12
·1-11
·
IF 6.408
Wang Jing,Xu Wei,Zhou Lin,Zhang Tianqi,Yang Na,Wang Min,Luo Xuemei,Jin Lu,Zhu Huaijun,Ge Weihong
The peroxidase-like activity of MXene@Fe 3 O 4 nanocomposites and the relevant colorimetric application have been investigated for the first time. According to a kinetic study, MXene@Fe 3 O 4 nanocomposites displayed higher peroxidase activity than their individual components, and the catalytic process followed a ping-pong mechanism. The improved peroxidase-like activity of the MXene@Fe 3 O 4 nanocomposites was obtained due to the synergetic impact of the Fe 3 O 4 and MXene phases, the huge ion-accessible interface, and quick electron transfer channels in the nanocomposites. In the presence of hydrogen peroxide (H 2 O 2 ), the MXene@Fe 3 O 4 nanocomposites can catalyze the reaction of the colorless substrate 3, 3, 5, 5-tetramethylbenzidine (TMB) into oxidized TMB which revealed a blue color at 652 nm. After the introduction of glutathione (GSH), the blue color was gradually fading owing to the hydroxyl radical scavenging effect of glutathione. A colorimetric detection platform based on the peroxidase-like activity of the MXene@Fe 3 O 4 nanocomposites was developed for H 2 O 2 and GSH with a detection limit of 0.4 μM and 0.5 μM, respectively. The calibration plot for glutathione detection is calculated by absorbance difference at 652 nm, which ranged from 0.5 to 10 μM with a detection limit of 0.2 μM. The recoveries of GSH with different concentrations ranged from 93.76 to 108.50% and the relative standard deviation from 0.30 to 5.96%. This work significantly extends the application of MXene@Fe 3 O 4 nanocomposites in the construction of colorimetric sensors and reveals a satisfactory result in real sample analysis. Graphical Abstract
研究领域:
纳米材料
·
分析化学
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生物传感器
·
催化
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产品关联
DOI
PubMed
高影响因子
Biosensors-Basel
·
2023
·Vol. 13
·期 8
·827
·
IF 5.4
Meixuan Liu,Chen Yan,Qianyun Ye,Xiaohuan Sun,Jie Han
Glutathione (GSH) is the most abundant low-molecular-weight biological thiol in vivo and has been linked to several diseases. The accurate quantification of GSH is therefore crucial for disease diagnosis and monitoring. In this study, we prepared self-assembled Cu(I)-Cys (cysteine) nanozymes through a two-step procedure. The Cu(I)-Cys nanoparticles exhibited peroxidase-mimicking activity. Upon the addition of H2O2, they were able to oxidize 3,3,5,5-tetramethylbenzidine (TMB) into oxTMB, resulting in a measurable increase in UV-Vis absorption at 655 nm. However, in the presence of GSH, oxTMB was reduced back to TMB, leading to a decrease in UV-Vis absorption at 655 nm. By utilizing these changes in the absorption intensity, we achieved the sensitive detection of GSH with a detection limit of 2.13 μM. Moreover, taking advantage of the different peroxidase-mimicking activities of Cu(I)-Cys nanoparticles at various pH values, a sensor array with Cu(I)-Cys nanoparticles at pH 4 and pH 5 was constructed. The discrimination of GSH among Cys and ascorbic acid was achieved and the practicability of the sensor array in human serum was validated. This novel approach holds significant promise for the precise discrimination and quantification of GSH and its potential applications in disease diagnosis and therapeutics.Keywords:nanozyme;GSH quantification;GSH discrimination
研究领域:
生物化学
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分析化学
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纳米技术
·
诊断学
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产品关联
DOI
PubMed
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2023
·
IF 9.9
Min Jia,Fupei Xu,Fei Zhai,Xiaoying Yu,Meixia Du
BPA aptamers functionalized cerium oxide nanoparticles encapsulated in zeolitic imidazolate framework-8 (CeO 2 @ZIF-8/Apt) were developed to fabricate an all-in-one portable platform for on-site quantitative detection of BPA. By combining biocomposites with a 3,3′,5,5′-tetramethylbenzidine (TMB)-based sodium alginate (SA) hydrogel and smartphone-based RGB analysis, highly sensitive and convenient monitoring of BPA was achieved. CeO 2 @ZIF-8 composites were constructed using a novel surfactant-modified concentration-controlled synthesis strategy. After being functionalized with BPA aptamers, CeO 2 @ZIF-8/Apt biocomposites were used as target-response colorimetric probes for target recognition and signal transduction. The oxidase-like activity of CeO 2 @ZIF-8 was effectively sealed by BPA aptamers and controllably released in a concentration-dependent manner through aptamer-BPA reactions. Utilizing SA hydrogels containing TMB in the caps, a one-step sample addition and one-pot detection can be conveniently achieved and reliably quantified by smartphone-based RGB analysis in an instrument-free way. The detection range of this portable detection platform is 50 pg/mL to 500 ng/mL with limit of detection calculated as 34.88 pg/mL, comparable to that of conventional detection in the solution system (4.57 pg/mL). The recoveries in tap water, apple juice, and milk ranged from 91.02 % and 106.75 %. This work contributes new insights into the design of all-in-one detection platforms for contaminants monitoring in resource-constrained regions.
研究领域:
环境科学
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分析化学
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纳米技术
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生物技术
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2023
·
IF 7.4
Xingxing Jiang,Shuping Liu,Xianwen Song,Qiang Li,Minghui Yang,Xiang Chen
How to overcome the intrinsic low activity of most oxidase and peroxidase mimics at neutral pH has been extremely challenging. Herein, we represent a chromium-mediated and ligand-dependent strategy to activate the oxidase-like activity of boron-doped g-C3N4 (B-g-C3N4, denoted as BG), aiming at breaking the pH limitation. Cr (III) can be in situ oxidized to Cr (IV) by generated •O2– upon UV light irradiation, which then works as a catalysis mediator to oxidize TMB under a neutral environment. Excitingly, the TMB oxidation can be rationally modulated by ligands on the BG coordinating with chromium. We verify that the PEI-Cr3+ coordination outperformed Cit-PEI-Cr3+ on the oxidase-like activity through a more accelerated electron transfer, unveiled by the Gauss theoretical calculations. This study highlights a paradigm of tuning the coordination environment on nanozyme surface via the ligand engineering strategy for boosting the oxidase-mimicking activity and breaking the pH limitation. Meanwhile, the catalysis-based colorimetric assay for accurate and selective identification of Cr3+ was achieved.
研究领域:
材料科学
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催化
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纳米技术
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分析化学
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环境科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PLoS One
·
2024
·Vol. 19
·期 5
·e0301358
·
IF 2.9
Chuang Wei,Yijun Gao,Peifeng Li
Drug-resistant bacteria arising from antibiotic abuse infections have always been a serious threat to human health. Killing bacteria with toxic reactive oxygen species (ROS) is an ideal antibacterial method for treating drug-resistant bacterial infections. Here, we prepared Pt-Ru bimetallic nanoclusters (Pt-Ru NCs) with higher peroxidase (POD)-like activity than Pt monometallic nanoclusters. Pt-Ru can easily catalyze the decomposition of H 2 O 2 to produce ·OH, thereby catalyzing the transformation of 3,3’,5,5’-tetramethylbiphenylamine (TMB) to blue oxidized TMB (oxTMB). We utilized the POD-like activity of the Pt-Ru NCs for antibacterial therapy. The results showed that at doses of 40 μg/mL and 16 μg/mL, the Pt-Ru NCs exhibited extraordinary antibacterial activity against E . coli and S . aureus , demonstrating the enormous potential of Pt-Ru NCs as antibacterial agents.
研究领域:
纳米技术
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生物材料
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抗菌研究
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产品关联
DOI
PubMed
高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Xianming Li,Ling Li,Heye Lv,Xiaoming Jiang,Peng Wu
Due to the unique features of 3,3′,5,5′-tetramethylbenzidine (TMB) in analytical chemistry, a series of TMB-based colorimetric nitrite assays (particularly nanozyme-involved) were developed to supplement the classical Griess assay. However, the exact reaction mechanisms and the final product between TMB and NO2– are controversial. Herein, we found that the widely adopted mechanism, namely the reaction between NO2– and the one-electron oxidation product of TMB (TMB+•), may be not valid. Alternatively, we found the reaction between NO2– and TMB primarily proceeded via diazotization (confirmed with ESI-MS) and yielded yellow diazo-TMB (not TMB2+, the two-electron oxidation product of TMB). In the presence of an easily ignored condition of visible light, diazo-TMB was promoted to its excited state, followed by two photoreactions to yield blue TMB+•, namely photoreduction ([diazo-TMB]* + TMB → TMB+•) and photosensitized oxidation ([diazo-TMB]* + O2 → 1O2, 1O2 + TMB → TMB+•). To facilitate the colorimetric process, a blue LED with a lighting wavelength matching with the maximum absorption of diazo-TMB, was employed to accelerate the generation of TMB+•. The light-driven colorimetric nitrite assay offered a limit of detection (LOD) of 0.1 μM, which was further explored to nitrite detection in urine samples and diagnosis of urinary tract infections caused by Escherichia coli.
研究领域:
分析化学
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环境化学
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生物分析化学
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光化学
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临床诊断
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产品关联
DOI
PubMed
高影响因子
Journal of Biomedical Nanotechnology
·
2022
·Vol. 18
·期 11
·2484-2497
·
IF 3.641
Li Guiyin,Chen Min,Li Haimei,Li Xinhao,Yao Xiaoqing,Li Liuxun
Golgi protein 73 (GP73) is a novel biomarker for the detection of hepatocellular carcinoma. We prepare a colorimetric aptasensor for GP73 detection based on the catalytic performance of the H-rGO-Mn3O4 nanozyme. The H-rGO-Mn3O4 nanozyme serves
as a peroxidase mimetic which can enhance the reaction with the substrate (3, 3,’ 5, 5′-tetramethylbenzidine, TMB) and H2O2. The peroxidase-like activity of H-rGO-Mn3O4 features a 1.78-fold higher binding affinity value (Km
)
for TMB compared with that of horseradish peroxidase. The H-rGO-Mn3O4 nanozyme not only exhibits admirable peroxidase-like activities due to the synergistic effect of Mn3O4 NPs, rGO and hemin, but also has a large specific surface area to endow the
GP73 aptamer with specific recognition capabilities. A sandwich colorimetric aptasensor is formed to realize the visual detection of GP73 through catalyzing the H2O2-mediated oxidation peroxidase substrate TMB to oxidized TMB accompanied by a color change from colorless
to blue. Under optimal conditions, the logarithm of the GP73 concentration (0.05–50.0 ng/mL) shows a good linear relationship with the absorbance. The calibration equation is Y =−0.0383 lgC+0.4835, with R2 of 0.9964, and the lowest limit of detection is 36.94
pg/mL. Additionally, the content of GP73 in human serum samples is directly detected, and the relative standard deviation is 0.49–4.91%. Compared with the enzyme-linked immunosorbent assay method, the relative error is 0.23–3.61%. All in all, colorimetric aptasensor is demonstrated
to exhibit excellent specificity, stability, and reproducibility.
研究领域:
生物标志物研究
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纳米技术
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分析化学
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癌症诊断
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生物化学
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Jiaojiao Zhou,Wenjing Wang,Shuojun Li,Axiu Nie,Zhiyong Song,Mohamed F. Foda,Zhicheng Lu,Ling Zhao,Heyou Han
An innovative fluorescent and colorimetric dual-mode strategy based on Y-shaped DNA was proposed for the detection of rabies virus oligonucleotide. The cascade assembly of hairpin probes was initiated by the target, leading to the formation of Y-shaped DNA accompanied by freeing target to catalyze the next assembly process. The fluorescence recovery of the fluorophore FAM was accompanied by opening of hairpin probe. A colorimetric signal was generated by the proximity of glucose oxidase (GOx) and horseradish peroxidase (HRP), which was reflected by enhanced catalytic capability to TMB. Using this method, target DNA can be detected with a wide linear range from 0.1 to 6 nM with a detection limit of 15 pM, and from 0.5 to 60 nM with a detection limit of 60 pM for fluorescent and colorimetric readout, respectively. Moreover, the dual-mode detection strategy exhibited excellent selectivity towards the target compared with interference oligonucleotides. In addition, recoveries ranging from 96.0% to 106% with RSD values varying from 3.2% to 5.4%, and from 91.0% to 102% with RSD values varying from 3.3% to 7.1% for fluorescent and colorimetric studies, respectively, were obtained in diluted cerebrospinal fluid samples. The proposed dual-mode sensor is easy to operate with excellent detection performance. It would be superior to single mode detection and more practicable for quantitative detection of nucleic acid-related biomarkers due to the self-correction capability.
研究领域:
生物化学
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分子生物学
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分析化学
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病毒学
✓ 已验证
产品关联
DOI
高影响因子
COLLOIDS AND SURFACES B-BIOINTERFACES
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2019
·
IF 3.973
Wenning Yang,Jia Li,Mingyue Wang,Xiaofan Sun,Yong Liu,Jie Yang,Dickon H.L. Ng
A novel core-shell Fe 3 O 4 /CoFe-LDH (layered double hydroxides) hybrid as a peroxidase mimic for the colorimetric detection of ascorbic acid was first fabricated via a facile two-step route. The resulting Fe 3 O 4 /CoFe-LDH hybrid exhibited much higher peroxidase-like catalytic activity for the oxidation of 3,3′,5,5′- tetramethylbenzidine (TMB) with H 2 O 2 than the pristine Fe 3 O 4 and CoFe-LDH nanosheets owing to the unique hierarchical architecture containing more exposed active sites and the synergistic effect between Fe 3 O 4 and CoFe-LDH. A sensitively and selectively visual sensor for the determination of ascorbic acid (AA) was successfully constructed based on the reduction effect of AA with enediol group on the formed oxidation of TMB, which exhibited a sensitive response to AA in the range of 0.5 ∼ 10 μM with the detection limit of 0.2 μM. Additionally, the Fe 3 O 4 /CoFe-LDH magnetic hybrid could be easily recycled by applying a magnetic field. This work provided a feasible means for the fabrication of magnetic nanomaterials with encouraging prospect in biosensing, environmental monitoring and medical diagnostics.
研究领域:
材料科学
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分析化学
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纳米技术
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生物传感器
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DYES AND PIGMENTS
·
2022
·
IF 5.122
Chengpei Du,Lin Qi,Yi Wang,Kanglin Pei,Renwen Zhang,Di Wu,Wenjing Qi
A fluorescent dual “turn-on” method is utilized to detect acid phosphatase (ACP) via dual quenching and fluorescence resonance energy transfer (FRET) strategy to improve sensitivity for the first time. Bovine serum albumin protected gold nanoclusters (BSA-AuNCs) are utilized as fluorescence probe and peroxidase-mimicking catalyst. BSA-AuNCs oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) to TMB (oxTMB) with the absorbance peak at 652 nm. Fluorescence spectrum of BSA-AuNCs with emission peak at 635 nm is largely overlapped with absorption spectrum of oxTMB, which brings FRET from BSA-AuNCs to oxTMB and fluorescence is quenched. When Ce 3+ is added, BSA-AuNCs catalyze the oxidation of Ce 3+ to Ce 4+ by H 2 O 2 . Ce 4+ has oxidizing properties, which can oxidize TMB into oxTMB and enhance FRET. It achieves the fluorescence is quenched further more. This dual fluorescence quenching effect brings a low background for ACP detection. ACP catalyzes ascorbic acid 2-phosphate (AA2P) hydrolysis into phosphate ion (PO 4 3− ) and ascorbic acid (AA). Owing to high coordination between Ce 3+ and PO 4 3− , TMB oxidation is prevented and fluorescent is turned-on. AA reduces oxTMB into TMB, which makes FRET be prevented and fluorescence is also increased. Therefore it is utilized in ACP detection from 0.01 to 2 U/L with high selectivity and accuracy.
研究领域:
分析化学
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生物医学工程
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产品关联
DOI
高影响因子
Reaction Chemistry & Engineering
·
2023
·Vol. 9
·期 1
·70-81
·
IF 3.9
Yuxuan Shao,Dan You,Yuqi Wan,Qingrong Cheng,Zhiquan Pan
The activation capacity of molecular oxygen is an important indicator to evaluate the photocatalytic efficiency of a catalyst. In this paper, MoO3−x nanosheets with oxygen vacancies were deposited on WO3 nanoflowers to form a WOM heterojunction, which improved the photocatalytic performance and molecular oxygen activation ability of the catalyst. This novel WOM heterojunction exhibited excellent photoactivity for degrading rhodamine B (RhB), photocatalytic water splitting ability and molecular oxygen activation ability under sunlight irradiation. Among all the samples, WOM83% could degrade 98% of 30 ppm RhB in 40 min. Meanwhile, WOM83% exhibited the highest hydrogen generation rate (4214.2 μmol h−1 g−1) and the strongest TMB oxidation capacity, and can generate 2.23 μmol of ·O2− in 50 min. The enhanced photocatalytic performance via heterojunction construction may be attributed to these following reasons: (i) higher light absorption achieved by the MoO3−x and WO3 composite; (ii) the matched energy band gap of MoO3−x and WO3 led to higher photogenerated carrier mobility; (iii) MoO3−x with oxygen vacancies as electron traps suppressed the photogenerated carrier recombination; (iv) enhanced molecular oxygen activation ability of the WOM heterojunction, such as the production of ·O2−. The measurements for TMB photo-oxidation and ·O2− showed excellent molecular oxygen activation ability of WOM heterojunctions. Finally, a possible photocatalytic mechanism was proposed. This study provided a viable option for the application of materials with oxygen vacancies to remove pollutants and improve molecular oxygen activation ability.
研究领域:
材料科学
·
光催化
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环境科学
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纳米技术
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2021
·
IF 4.098
Yanyun Zou,Qingli Chai,Ting Zhu,Xiaoxiao Yu,Guobin Mao,Ningxing Li,Jinyang Chen,Guosong Lai
In this work, an environmentally-friendly and cost-effective enzyme mimic was obtained by facile one-pot preparation of chitosan/Cu/Fe (CS/Cu/Fe) composite. This composite exhibited significantly enhanced oxidase-mimicking activity during catalyzing the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB). The CS/Cu/Fe composite was comprehensively characterized and the possible catalytic mechanism was reasonably explored and discussed. Benefiting from the thermal stability and the compatibility with carbohydrate, the CS/Cu/Fe composite was further integrated with agarose hydrogel to fabricate a portable analytical tube containing oxidase mimic. Based on the inhibition of the catalytic oxidation of TMB in the presence of cysteine, as well as the recovery of oxidase-like activity of CS/Cu/Fe due to the specific complexation of cysteine and mercury ion (Hg 2+ ), the rapid colorimetric detection of Hg 2+ was successfully carried out in the analytical tube. This colorimetric method showed good linear response to Hg 2+ over the range from 40 nM to 8.0 μM with a detection limit of 8.9 nM. The method also revealed high selectivity and satisfactory results in recovery experiments of Hg 2+ detection in tap water and lake water. Furthermore, it was found that the effective removal of Hg 2+ could be realized in the analytical tube based on efficient Hg 2+ adsorption by CS/Cu/Fe composite and agarose hydrogel. This study not only prepared a robust and low-cost enzyme mimic, but also proposed a smart strategy to simultaneously monitor and remove toxic Hg 2+ from contaminated water.
研究领域:
环境科学
·
分析化学
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材料科学
·
水处理
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）