IET Nanobiotechnology
·
2020
·Vol. 14
·期 2
·167-171
·
IF 1.859
Xiyuan Tong,Shiyao Jin,Yingdai Zhao,Yue Gai,Yifeng E,Dan Li
The electrochemiluminescence (ECL) system based on the ruthenium complex has become a powerful tool in the field of analytical chemistry. However, the non-aqueous ECL luminescence system, which does not involve complex nano-modification, has not been widely used for the determination of analytes. In this study, N -methyl pyrrolidone was selected as the solvent, and it could also act as a co-reactant of . Based on this, a simple ECL system without nanomaterials was established. Strong ECL was generated. Furthermore, a quenching effect between the excited state of and sulphamethoxazole (SMZ) was observed. Based on this, a sensitive ECL sensor for detecting SMZ is constructed. A linear relationship between ECL signal quenching intensity (Δ I ) and the logarithm of SMZ concentration (log C ) in the concentration range of 1 × 10 −7 –1 × 10 −5 mol/l is obtained. The limit of detection is as low as 3.33 × 10 −9 mol/l. The method has been applied to the detection of SMZ in tap water samples with different concentration levels with satisfactory results, and the recovery was 95.3–102.6%.
研究领域:
分析化学
·
生物传感技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ELECTROCHIMICA ACTA
·
2022
·
IF 7.336
Fangxin Du,Zhiyong Dong,Fangshuo Liu,Saima Anjum,Morteza Hosseini,Guobao Xu
A single-electrode electrochemical system (SEES) has been developed by attaching a plastic sticker with 24 holes onto tris(1,10-phenanthroline)ruthenium (Ru(phen) 3 2+ ) modified carbon nanotube (CNT)/graphene film electrode for the first time. The Ru(phen) 3 2+ modified CNT/graphene film electrode was fabricated by using CNT/graphene ink with the addition of Ru(phen) 3 2+ . The immobilization of Ru(phen) 3 2+ is achieved through the π-π stacking interaction between Ru(phen) 3 2+ and CNT/graphene. The potential difference between the ends of cells in SEES is induced upon applying voltage because of the resistance of the Ru(phen) 3 2+ modified CNT/graphene film electrode, and triggers Ru(phen) 3 2+ electrochemiluminescence (ECL) reaction. By using a smart phone as the detector, visual ECL analysis of multiplex samples was achieved on this newly developed system. Dopamine (DA) can quench triethylamine ECL at Ru(phen) 3 2+ modified CNT/graphene film electrode, enabling visual DA detection. The visual DA detection demonstrates a good linearity in the range of 10 −3 –10 μM and 50–500 μM. The limit of detection is calculated as 1.9 nM at a signal-to-noise ratio of 3. The ECL system also exhibits excellent long-term stability after storage in the ambient environment for 60 days by comparing the ECL response with its original signal.
研究领域:
分析化学
·
材料科学
·
生物医学工程
✓ 已验证
DOI
高影响因子
ENVIRONMENTAL RESEARCH
·
2022
·
IF 8.431
Siyuan Chen,Haiming Cai,Xiaodong Du,Peiwen Wu,Xueqin Tao,Jiangmin Zhou,Zhi Dang,Guining Lu
Tris(2-chloroethyl) phosphate (TCEP) as a new type of flame retardant exists in various water environments, causing great risks to humans and the environment. In this study, shrimp shell was used to prepare an economical and environmental-friendly adsorbent for the efficient removal of TCEP. The systematic studies including characterization, removal performance, and adsorption mechanism of shrimp shell biochar toward TCEP were carried out. Adsorption kinetics and thermodynamics showed that fast equilibrium reached within 30 min, the maximum adsorption capacity q m was 108 μmol g −1 at 298 K, and the adsorption process is spontaneous and exothermic. The environmental factor, such as temperature, pH, inorganic anions and organic matter hardly affected the adsorption performance. Structural characterization indicated that the hierarchical porous structure of shrimp shell biochar is the key to excellent adsorption performance. The adsorption mechanisms were further revealed using density functional theory (DFT) calculations, and the hydrogen bond , van der Waals interactions, Cl–H interactions, and pi-H interactions were identified as potential interaction mechanisms between TCEP and specific biochar structures. The calculated binding energy between TCEP and simplified biochar structure suggested that oxygen-containing groups especially carboxyl, hydroxyl and aldehyde facilitate the adsorption. Our work not only provides a novel strategy for the quick remediation of organophosphate-contaminated water environments but also offers new opportunities for crustacean waste biomass valorization.
研究领域:
环境科学
·
材料科学
·
化学工程
·
水处理
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Small
·
2024
·
IF 13
Leqi Li,Xinyuan Ye,Zichong Ji,Meiqiong Zheng,Shihong Lin,Mingzhe Wang,Jiawei Yang,Pengcheng Zhou,Zongman Zhang,Binghao Wang,Haoyang Wang,Yan Wang
High-fidelity wireless electrophysiological monitoring is essential for ambulatory healthcare applications. Soft solid-like hydrogels have received significant attention as epidermal electrodes because of their tissue-like mechanical properties and high biocompatibility. However, it is challenging to develop a hydrogel electrode that provides robust contact and high adhesiveness with glabrous skin and hairy scalp for high-fidelity, continuous electrophysiological signal detection. Here, a paintable, fast gelation, highly adhesive, and conductive hydrogel is engineered for high-fidelity wireless electrophysiological monitoring. The hydrogel, consisting of gelatin, gallic acid, sodium citrate, lithium chloride, glycerol, and Tris-HCl buffer solution exhibits a reversible thermal phase transition capability, which endows it with the attributes of on-skin applicability and fast in situ gelation with 15 s, thereby addressing the aforementioned limitations. The introduction of gallic acid enhances the adhesive properties of the hydrogel, facilitating secure electrode attachment to the skin or hairy scalp. To accentuate the potential applications in at-home electrophysiological health monitoring, the hydrogel electrodes are demonstrated for high-fidelity electrocardiogram recording for one hour during various daily activities, as well as in simultaneous electroencephalogram and electrocardiogram recording during a 30 min nap.
研究领域:
生物医学工程
·
材料科学
·
电生理学
·
医疗技术
✓ 已验证
产品关联
DOI
PubMed
高影响因子
INORGANICA CHIMICA ACTA
·
2021
·
IF 2.545
Xingliang Li,Juncai Lu,Wanjun Mu,Baihua Chen,Daibing Luo,Bijun Liu,Yanqiu Yang,Hongyuan Wei,Shuming Peng
Nitrilotri–3–propanoic Acid (NTPA) and Tris(2–carboxyethyl) phosphine (TCEP) possess similar skeleton. This pair of isostructural ligands is a perfect set to assess the differences of uranyl (UO 2 2+ ) complexes with N–tricarboxylate versus P–tricarboxylate. The protonation reactions of NTPA and TCEP were investigated by potentiometry and calorimetry. Complexation of uranyl with the both ligands was studied by potentiometry and structural analysis. The results indicate that central trialkylamine in NTPA has a higher affinity for the proton than trialkylphosphine in TCEP. Crystal structures and NMR analyses indicate that central amine of NTPA and central phosphine of TCEP are not involved in the bonding. NTPA/UO 2 2+ complex is more stable than TCEP/UO 2 2+ complex. This difference cannot be explained by electronic effect of central N and P atoms because of nonbonding interactions. The higher stability of UO 2 2+ /NTPA versus UO 2 2+ /TCEP may be due to the fact that the central N atom of NTPA has a lower steric hindrance than the central P atom of TCEP. Another possible reason is that UO 2 2+ /TCEP complexes have different coordination modes from UO 2 2+ /NTPA complexes.
研究领域:
化学
·
配位化学
·
核化学
✓ 已验证
DOI
高影响因子
ChemElectroChem
·
2022
·Vol. 9
·期 12
·e202200236
·
IF 4.782
Jialian Ding,Ping Zhou,Bin Su
Graphical Electrochemiluminescence quantum efficiency (ECL-QE) : ECL-QE of Ru(bpy) 3 2+ /TPrA system was calculated in terms of a kinetic reaction model, which could reveal the root cause of ECL-QE change and could potentially be used as a criterion of selecting efficient luminophores and analytical conditions. Electrochemiluminescence quantum efficiency (ECL-QE) is an important parameter to evaluate the energy conversion ability of luminophores and analytical sensitivity of corresponding ECL systems. Herein, taking tris(2,2’-bipyridine)ruthenium(II) (Ru(bpy) 3 2+ ) and tri- n -propylamine (TPrA) coreactant system as an example, the ECL-QE was evaluated in terms of a kinetic reaction model, in which Ru(bpy) 3 3+ was considered to be the key intermediate in a reaction scheme consisting of competitive oxidative reduction and catalytic reaction routes. It was calculated as the product of electrochemical excitation efficiency ( η ex ) and light emission efficiency (i. e., photoluminescence quantum yield, PLQY). We used finite element simulations to examine the variation of η ex with the electrode material, applied potential, concentration and distance from the electrode surface. η ex was found to be largely determined by the electrochemical oxidation rate of TPrA that varied by several orders of magnitude with the electrode material. The estimation of ECL-QE helped in selecting the appropriate conditions for efficient ECL generation and sensitive analysis.
研究领域:
分析化学
·
电化学
✓ 已验证
DOI
高影响因子
ACS Agricultural Science & Technology
·
2025
·
IF 2.9
Huiqian Wang,Dongru Wang,Qiuping Zheng,Yuan He,Qian Yang,Jiani Du,Jiawei Wang,Xinhua Zhan
Polyvinyl chloride microplastics (PVC-MPs) pollution is drawing increasing attention, especially due to concerns about the environmental safety of the diverse additives that they contain. To date, few ecotoxicity data are available for PVC MP-derived chemicals. Here, wheat (Triticum aestivum L.) seedling roots were used to comparatively evaluate the toxicity of three commonly studied PVC MP-derived chemicals (2,4-dimethyl-6-s-hexadecylphenol─Irganox 1076 and tris (2,4-ditert-butylphenyl) phosphate─Irgafos 168-ox, and erucamide─Eru). These chemicals were evaluated individually and in combination. Exposure to these chemicals caused dose-dependent reductions in root dry weight (0.39%–19.29%) and root length (0.11%–8.15%). While Irganox 1076 and Eru had minimal impact on root activity and the antioxidant system, Irgafos 168-ox and its mixture induced significant elevations of antioxidant enzymes (SOD, CAT, and APX) activities and antioxidants (AsA and GSH) concentrations in the roots, enhancing the antioxidant level. Our findings indicate that the coexposure of PVC MP-derived chemicals exerts an additive effect on antioxidant response inhibition. Irgafos 168-ox showed stronger effects with the maximum toxic concentration of 10 mg L–1, whether alone or in additive mixtures, due to its higher potential for root accumulation and oxidative stress induction. These results highlight the need for further research into ecological risks of PVC MP-derived chemicals, particularly under combined exposure scenarios.
研究领域:
环境科学
·
植物生理学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THERMAL ANALYSIS AND CALORIMETRY
·
2024
·
IF 3
Tang Gang,Hu Yang,Fang Yuan,Guan Mengfan,Gong Zedong,Wang Guodong,Deng Dan,Liu Xiuyu
A tris(DOPO-grafted piperazine)-triazine phosphoramide (DOPO-TPT) was synthesized, and its structure was well characterized by Fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy. DOPO-TPT was used as a flame retardant additive to modify the epoxy resin to obtain a series of flame retardant epoxy thermosets. The effects of DOPO-TPT on the thermomechanical properties, thermal stability and flame retardancy of the resulting epoxy thermosets were investigated. The results showed that when the addition of DOPO-TPT was 5 mass% (phosphorus content was 0.47 mass%), the limiting oxygen index of the epoxy thermoset reached 30.0%, and it passed the UL-94 V-0 classification. Compared with unmodified epoxy resin, the maximum heat release rate and total heat release of EP/DOPO-TPT-5.0 decreased by 28% and 20%, respectively. Additionally, the presence of DOPO-TPT did not deteriorate the thermomechanical properties of the resulting epoxy thermosets. TG-FTIR test confirmed that the incorporation of DOPO-TPT could significantly inhibit the release of gaseous products. The char layer analysis results showed that DOPO-TPT had good catalytic charring performance, which was conducive to decreasing the escape of combustible gas during combustion, thus improving the flame retardancy.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
CHEMICO-BIOLOGICAL INTERACTIONS
·
2024
·
IF 4.7
Wenwen Luo,Keqi Hu,Yijing Chen,Lin Wang,Yungang Liu
Tris(2-butoxyethyl) phosphate (TBOEP) is an organophosphorus flame retardant ubiquitously present in the environment and even the human body. TBOEP is toxic in multiple tissues, which forms dealkylated and hydroxylated metabolites under incubation with human hepatic microsomes; however, the impact of TBOEP metabolism on its toxicity, particularly mutagenicity (typically requiring metabolic activation), is left unidentified. In this study, the mutagenicity of TBOEP in human hepatoma cell lines (HepG2 and C3A) and the role of specific CYPs were studied. Through molecular docking, TBOEP bound to human CYP1A1, 1B1, 2B6 and 3A4 with energies and conformations favorable for catalyzing reactions, while the conformations of its binding with human CYP1A2 and 2E1 appeared unfavorable. In C3A cells (endogenous CYPs being substantial), TBOEP exposing for 72 h (2-cell cycle) at low micromolar levels induced micronucleus, which was abolished by 1-aminobenzotriazole (inhibitor of CYPs); in HepG2 cells (CYPs being insufficient) TBOEP did not induce micronucleus, whose effect was however potentiated by pretreating the cells with PCB126 (CYP1A1 inducer) or rifampicin (CYP3A4 inducer). TBOEP induced micronucleus in Chinese hamster V79-derived cell lines genetically engineered for stably expressing human CYP1A1 and 3A4, but not in cells expressing the other CYPs. In C3A cells, TBOEP selectively induced centromere protein B-free micronucleus (visualized by immunofluorescence) and PIG-A gene mutations, and elevated γ-H2AX rather than p-H3 (by Western blot) which indicated specific double-strand DNA breaks. Therefore, this study suggests that TBOEP may induce DNA/chromosome breaks and gene mutations in human cells, which requires metabolic activation by CYPs, primarily CYP1A1 and 3A4.
研究领域:
毒理学
·
分子生物学
·
细胞生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ENVIRONMENTAL POLLUTION
·
2025
·
IF 7.3
Ziyuan Li,Pu Chen,Yongfeng Lin,Jingxu Zhang,Jian Ding,Rifat Zubair Ahmed,Xiaoting Jin,Yuxin Zheng
Tris (2-chloroethyl) phosphate (TCEP), recognized as an emerging pollutant, has been frequently detected in human blood. Maintenance of blood homeostasis is indispensable for regulating various physiological states and overall health, yet hematological toxicology of TCEP has not been extensively investigated. Platelets, a vital component of blood, are fundamental in the processes of hemostasis and thrombosis through their activation; thus, this study was designed to elucidate the effects and underlying mechanisms of TCEP on platelet activation. Utilizing an in vivo model, we conducted a proteomic analysis of platelets and found that TCEP exposure inhibited platelet activation. An ex vivo platelet evaluation system was employed to further dissect the processes of platelet activation, revealing that TCEP predominantly suppressed platelet aggregation, degranulation and clot retraction. These processes were highly dependent on energy metabolism, and TCEP was found to decrease ATP levels, primarily by impairing glycolysis and pentose phosphate pathways. Subsequent investigation into the molecular mechanisms revealed that TCEP decreased the activity of phosphofructokinase platelet (PFKP) by enhancing O-linked N-acetylglucosamine (O-GlcNAc) transferase interaction with PFKP. This study is the first to uncover the disruptive effects of TCEP on platelet activation process, providing valuable insights into the assessment of hematologic health risks associated with TCEP-like emerging pollutants exposure.
研究领域:
血液学
·
毒理学
·
分子生物学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Desalination and Water Treatment
·
2024
·
IF 1
Qinglong Luo,Lei Liu,Ruiqiang Dong,Zhongfu Cheng,Mingzhe Dong,Zhijian Wu,Jun Li,Xueli Huang
ABSTRACT The removal of boron from aqueous solution and the enrichment of boron from salt lake have attracted more attention. In this work, organo-inorganic hybrid mesoporous boron adsorbents were synthesized by tris(hydroxymethyl)methyl aminomethane (TRIS) grafted onto the meso-porous SBA-15, SBA-16 and MCM-41, respectively. And the structures are characterized by infrared, scanning electron microscopy, energy-dispersive X-ray, mapping, X-ray diffraction, point of zero charge, and Marvin laser particle size analyzer. The results showed that TRIS was successfully grafted onto SBA-15, SBA-16, and MCM-41. The adsorption isotherm and adsorption kinetics were best fitted with Langmuir model and pseudo-second-order rate equation, respectively. The adsorption capacity of the three adsorbents was 15.28, 15.70 and 14.17 mg/g, respectively. And the same functional group has little influence on the boron adsorption capacity of different carrier adsorbents of the same type (organosilicon material). These adsorbents have highly selective adsorption of boron, and maintain good lifetime after adsorption–desorption for 5 times, and the dissolution loss rate of SBA-16-TRIS after ten cycles was 0.93%.
研究领域:
环境科学
·
材料科学
·
化学工程
✓ 已验证
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2019
·
IF 6.393
Ning Xia,Dehua Deng,Xiangsheng Mu,Ang Liu,Jiaxun Xie,Dandan Zhou,Ping Yang,Yun Xing,Lin Liu
This work reported a novel redox cycling in which pyrroloquinoline quinone (PQQ) promoted the reduction of Fe(III)-ferrozine by tris(2-carboxyethyl)phosphine (TCEP). Specifically, PQQ was reduced into pyrroloquinoline quinol (PQQH 2 ) by TCEP, and then the produced PQQH 2 reduced colorless Fe(III)-ferrozine into dull red Fe(II)-ferrozine. The redox cycling was employed for development of colorimetric immunosensors through the generation of colorful Fe(II)-ferrozine. Mesoporous silica nanoparticle (MSN) was used as the carrier for both PQQ and recognition antibody through electrostatic interactions. Magnetic bead (MB) was used as the support for the immobilization of capture antibody. After the sandwich-type immunoreactions and magnetic separation, the MSN-PQQ nanolabels on the MB surface triggered the production of Fe(II)-ferrozine. The limit of detection was found to be 1 pg/mL with prostate specific antigen (PSA) as the model target. The result for the analysis of serum sample is in agreement with that achieved by the commercial enzyme-linked immunosorbent assay kits. The proposed immunosensor obviated the use of enzyme molecules for signal amplification and did not require expensive instruments for signal readout. This work should be valuable for the design of novel nanolabels and the proposed sensing strategy by the redox cycling could be applied to develop more sensitive biosensors.
研究领域:
分析化学
·
纳米技术
·
生物传感器
·
免疫学
✓ 已验证
产品关联
DOI
高影响因子
危险品化学品经营许可证（带存储）