Biosensors-Basel
·
2023
·Vol. 13
·期 1
·118
·
IF 5.4
Yue Guan,Junning Ma,Jing Neng,Bolei Yang,Yan Wang,Fuguo Xing
Zearalenone (ZEN), one of the most frequently occurring mycotoxin contaminants in foods and feeds, poses considerable threat to human and animal health, owing to its acute and chronic toxicities. Thus, rapid and accurate detection of ZEN has attracted broad research interest. In this work, a novel and label-free chemiluminescence aptasensor based on a ZEN aptamer and a G-quadruplex DNAzyme was constructed. It was established on a competitive assay between ZEN and an auxiliary DNA for the aptamer, leading to activation of the G-quadruplex/hemin DNAzyme and subsequent signal amplification by chemiluminescence generation after substrate addition. To maximize the detection sensitivity, numerous key parameters including truncated aptamers were optimized with molecular docking analysis. Upon optimization, our aptasensor exhibited a perfect linear relationship (R2= 0.9996) for ZEN detection in a concentration range of 1–100 ng/mL (3.14–314.10 nM) within 40 min, achieving a detection limit of 2.85 ng/mL (8.95 nM), which was a 6.7-fold improvement over that before optimization. Most importantly, the aptasensor obtained a satisfactory recovery rate of 92.84–137.27% and 84.90–124.24% for ZEN-spiked wheat and maize samples, respectively. Overall, our label-free chemiluminescence aptasensor displayed simplicity, sensitivity, specificity and practicality in real samples, indicating high application prospects in the food supply chain for rapid detection of ZEN.Keywords:chemiluminescence;label-free;aptasensor;DNAzyme;ZEN
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LANGMUIR
·
2023
·
IF 3.9
Yan Zhao,Xiaoyu Han,Na Zhang,Junping Meng,Hong Zhang,Jinsheng Liang
In view of the animal feeds inevitably contaminated by multiple mycotoxins, eco-friendly and efficient palygorskite-montmorillonite (Pal-Mt) materials were prepared to remove polar aflatoxin B1 (AFB1) and weak polar zearalenone (ZEN) from mixed mycotoxins aqueous solution. The adsorption properties and bonding mechanisms between Pal-Mt materials and mycotoxins (AFB1 and ZEN) were investigated systematically. The as-prepared Pal-Mt showed excellent adsorption capacity for AFB1 and ZEN in single- and binary-mycotoxin systems, indicating the effectiveness of Pal-Mt acting as multiple mycotoxin adsorbents. The kinetics of adsorption for ZEN was fast due to the adsorption on the external surface (film and intraparticle diffusion), while AFB1 molecules permeated into mesopores after the external adsorption for the more planar structure. Adsorption isotherms demonstrated that heterogeneous surface adsorption appeared between Pal-Mt and AFB1, and monolayer adsorption occurred on Pal-Mt and ZEN for different polarities of mycotoxins. Thermodynamic parameters illustrated that the adsorption process of both AFB1 and ZEN onto Pal-Mt was spontaneous and endothermic. The adsorption mechanism studies suggested that hydrogen bonding, electrostatic attraction, calcium bridging linkage, and ion-dipole played fundamental roles in the interaction between Pal-Mt and these two mycotoxins.
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MICROCHEMICAL JOURNAL
·
2022
·
IF 5.304
Rentang Huang,Yuxin Huang,Hongmei Liu,Kaiyi Guan,Ali Chen,Xiangsheng Zhao,Shumei Wang,Lei Zhang
Herein, we present a bifunctional gold nanoparticles (AuNP) probe-based enzyme-linked immunosorbent assay (nano-ELISA) for detecting zearalenone (ZEN) in the popular edible and medicinal food coix seed. This nano-ELISA takes advantage of the convenient labeling property of AuNPs, which allows the horseradish peroxidase and antibody were successively immobilized onto AuNPs by electrostatic adsorption to construct a bifunctional probe for identification and signal amplification. In our model system, a time-saving direct competitive ELISA scheme was selected, in which mutual optimization of biomolecules, a two-stage process for incubation, and comparison of different particle sizes were performed to reach higher sensitivity. The proposed method achieves a limit of detection of 8 μg/kg and a limit of quantitation of 22 μg/kg, which is an approximately 7-fold and 5-fold improvement over the traditional ELISA, respectively. The calibration curve for the analyte was linear over the concentration range of 22–508 μg/kg. The recoveries for ZEN spiked coix seed samples ranged from 98.67 to 113.5 % with a relative standard deviation of less than 8 %. Moreover, the developed nano-ELISA could overcome the interference from the sample matrix. A good correlation was observed between the results from nano-ELISA and liquid chromatography-tandem mass spectrometry (r 2 = 0.9757, n = 69), which paves the useful and practicable way for accurate bioanalysis for trace hazardous substances in food.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Weili Qiao,Baoshan He,Jun Yang,Wenjie Ren,Renyong Zhao,Yurong Zhang,Chunqi Bai,Zhiguang Suo,Yiwei Xu,Min Wei,Huali Jin
Lateral flow immunoassay (LFIA) has been employed extensively for the rapid, accurate, and portable detection of foodborne toxins. Here, the platinum gold nanoflower core-shell (Pt@AuNF) nanozyme with excellent optical properties, good catalytic ability and controllable reaction conditions were prepared to effectively improve the performance of lateral flow immunoassay (LFIA) strips. The Pt@AuNF nanozyme and horseradish peroxidase (HRP) combined with monoclonal antibody were used as signal probes based on the dual enzymes catalytic signal amplification strategy to detect Zearalenone sensitively. Dual enzymes catalyze the decomposition of hydrogen peroxide into hydroxyl radicals, and under the influence of hydroxyl radicals, colorless 3,3′,5,5′ -tetramethylbenzidine (TMB) is oxidized to blue ox-TMB, which is superimposed on the strips for signal amplification to broaden the detection range. The limit of detection (LOD) of the Pt@AuNF-HRP labeled LFIA strips after signal amplification was 0.052 ng/mL, and the detection range was 0.052–7.21 ng/mL. Compared with the Pt@AuNF labeled strips, while reducing the probes amount by half to achieve antibody conservation, the detection range was expanded by 5-fold based on achieving improved sensitivity. The study provided a meaningful reference for expanding the detection range based on immunoassay.
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Toxins
·
2019
·Vol. 11
·期 8
·451
·
IF 3.895
Ming Li,Chuqin Yang,Yuhao Mao,Xia Hong,Daolin Du
In total, 405 samples of corn, corn products, and swine feed from China in 2016–2018 were surveyed for zearalenone (ZEN) contamination using a magnetic bead immunoassay-coupled biotin–streptavidin system (BAS-MBI). The developed BAS-MBI had a limit of detection (LOD) of 0.098 ng mL−1, with half-maximal inhibition concentration (IC50) of 0.71 ng mL−1in working buffer, and an LOD of 0.98 ng g−1; the detection range was from 0.98 to 51.6 ng g−1in authentic agricultural samples. The BAS-MBI has been demonstrated to be a powerful method for the rapid, sensitive, specific, and accurate determination of ZEN. The ZEN positivity rate reached the highest level of 40.6% in 133 samples in 2016; ZEN levels ranged from 1.8 to 1100.0 ng g−1, with an average level of 217.9 ng g−1. In 2017, the ZEN positivity rate was the lowest at 24.5% in 143 samples; ZEN levels ranged from 1.1 to 722.6 ng g−1, with an average of 166.7 ng g−1. In 2018, the ZEN positivity rate was 31.8% in 129 samples; ZEN levels ranged from 1.3 to 947.8 ng g−1, with an average of 157.0 ng g−1. About 20% of ZEN-positive samples exceeded maximum limit levels. An alternative method of ZEN detection and a valuable reference for ZEN contamination in corn and its related products in China are provided. This survey suggests the need for prevention of serious ZEN contamination, along with management for food safety and human health.Keywords:zearalenone;contamination;magnetic bead immunoassay;corn;corn product;swine feedKey Contribution:In total, 405 corn and related product samples from China in 2016–2018 were surveyed for zearalenone contamination using a high-efficiency magnetic bead immunoassay.
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RSC Advances
·
2013
·Vol. 4
·期 6
·2764-2771
·
IF 2.562
Guozhen Fang,Chao Fan,Huilin Liu,Mingfei Pan,Huidan Zhu,Shuo Wang
A novel molecularly imprinted optosensing material (MIOM) based on ionic liquid (IL)-stabilized CdSe/ZnS quantum dots (QDs) was prepared, for highly selective and sensitive recognition of the mycotoxin zearalenone (ZON). ZON is expensive and highly toxic, so the ZON analog cyclodo-decanyl-2,4-dihydroxybenzoate (CDHB) was instead used as the template. MIOM was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and adsorption experiment. Under optimal conditions, the relative fluorescence intensity of MIOM decreased linearly with increasing ZON concentration, in the range of 0.003–3.12 μmol L−1. MIOM had a detection limit of 0.002 μmol L−1. MIOM was used to detect ZON in corn, rice and wheat flours, and at three concentration levels 50, 100 and 500 ng g−1, ZON recoveries for these three cereals were 94.2–98.7%, 93.1–107.6% and 84.4–106.0%, respectively.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2017
·
IF 3.154
Xiya Zhang,Sergei A. Eremin,Kai Wen,Xuezhi Yu,Chenglong Li,Yuebin Ke,Haiyang Jiang,Jianzhong Shen,Zhanhui Wang
To develop a sensitive fluorescence polarization immunoassay (FPIA) for screening the zearalenone class of mycotoxins in maize, two new monoclonal antibodies with uniform affinity to the zearalenone class and four fluorescein-labeled tracers were prepared. After careful selection of appropriate tracer–antibody pairs in terms of sensitivity and specificity, a FPIA that could simultaneously detect the zearalenone class with similar sensitivity was developed. Under optimum conditions, the half maximal inhibitory concentrations of the FPIA in buffer were 1.89, 1.97, 2.43, 1.99, 2.27, and 2.44 μg/L for zearalenone, α-zearalenol, β-zearalenol, α-zearalanol, β-zearalanol, and zearalanone, respectively. The limit of detection of FPIA for the zearalenone class was around 12 μg/kg in maize, and the recoveries ranged from 84.6 to 113.8%, with coefficients of variation below 15.3% in spiked samples. Finally, the FPIA was applied for screening naturally contaminated maize samples, and the results indicated a good correlation with that of high-performance liquid chromatography–tandem mass spectrometry.
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JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Li-Hong Su,Hai-Long Qian,Shu-Ting Xu,Cheng Yang,Zhenyu Wang,Xiu-Ping Yan
Food and environment contaminations by zearalenone (ZEN) has aroused serious issues in public health. Sensitive and accurate detection of ZEN remains a daunting challenge due to complex interference and low residue in real samples. Herein, we report a novel molecular imprinted covalent organic framework with flexible aliphatic linkers (ALCOF-MIP) for rapid and selective extraction of ZEN in real samples. Flexible aliphatic linkers were employed to regulate the molecular conformation and enhance hydrophily, enabling ALCOF-MIP to possess high selectivity and fast kinetics for ZEN extraction. ALCOF-MIP with selective imprinting sites displayed the maximum adsorption capacity for ZEN (1250 mg g −1 , exceeding most of reported adsorbents). Furthermore, the ALCOF-MIP based solid-phase extraction coupled with high-performance liquid chromatography gave a wide linear range (1–500 ng mL –1 ), low detection limit (0.06 ng mL –1 ) and high precision (≤3.2 %). The recovery of spiked ZEN in real samples ranged from 90.7 % to 99.1 %. The proposed method is promising for monitoring mycotoxin residues and assessing food safety without matrix interferences.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Pengli Liu,Yayi Gu,Zhihao Xu,Hui Zou,Jianhong Gu,Yan Yuan,Zongping Liu,Jianchun Bian
Zearalenone (ZEA) is a mycotoxin that heavily contaminates feed and its raw materials, entering the body through dietary intake. Although it has been reported that ZEA causes liver damage, its toxic mechanisms are unclear. In this study, we first confirmed that ZEA induces liver injury in mice, triggering endoplasmic reticulum stress (ERS) and inflammation in vivo. Second, in vitro, we demonstrated that ZEA also causes ERS and inflammation in BRL 3A cells, with ERS playing a promoting role in ZEA-induced inflammation. Finally, we confirmed that ERS promotes ADAM17 expression in BRL 3A cells, and ADAM17 plays a facilitating role in ZEA-induced inflammation. In summary, these results indicate that ERS promotes ZEA-induced inflammation by upregulating ADAM17 expression. ADAM17 is a key target in ZEA-induced liver damage, and inhibition of ADAM17 represents a novel approach for treating liver damage. Furthermore, this study provides a new strategy for discovering effective therapeutic drugs.
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ANALYST
·
2021
·Vol. 146
·期 10
·3328-3335
·
IF 4.616
Jingzhi Li,Xudong Zhao,Yu Wang,Shuang Li,Yingkai Qin,Tie Han,Zhixian Gao,Hui Liu
Mycotoxins cause significant harm to human health, so it is imperative to develop a highly sensitive and easy-to-operate method for the detection of mycotoxins. Herein, a fluorescence-based magnetic separation immunoassay for simultaneous detection of mycotoxins fumonisin B1 and zearalenone is established. The method employed high fluorescent upconversion-nanoparticles(UCNPs) conjugated with biotinylated antigens as upconversion fluoroscent probes. Magnetic nanoparticles(MNPs) immobilized with monoclonal antibodies are used as immune-capture probes. Highly sensitive detection of FB1 and ZEN was achieved based on the luminescence properties of UCNPs and the separation effects of MNPs. The results showed a robust linear correlation between the enhanced fluorescence emission intensity and the logarithmic concentrations of FB1 and ZEN under the optimal conditions (R2(FB1) = 0.9965, R2(ZEN) = 0.9976), and the linear ranges were 0.05–5 ng mL−1. Furthermore, the limits of detection (LOD) were 0.016 ng mL−1 for FB1 and 0.012 ng mL−1 for ZEN. The standard addition method was used to determine the content of FB1 and ZEN in the samples to evaluate the accuracy of the process. The average recoveries were 89.48% to 113.69% and 85.97% to 113.82%, respectively. Compared with the other five mycotoxins, this method had high selectivity. It is expected that the multi-component simultaneous detection can be further realized by using the multicolor labeling characteristics of UCNPs.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2018
·
IF 3.412
Yuanyuan Zhang,Taofeng Lu,Yue Wang,Chenxi Diao,Yan Zhou,Lili Zhao,Hongyan Chen
Contamination of feed with zearalenone (ZEN) presents a significant risk to animal health. Here, a visible, rapid, and cost-effective aptamer-based method is described for the detection of ZEN. After 8 rounds of SELEX (systematic evolution of ligands by exponential enrichment) with an affinity-based monitor and counter-screening process, the ssDNA aptamer Z100 was obtained, which had high affinity (dissociation constant = 15.2 ± 3.4 nM) and good specificity. Docking analysis of Z100 indicated that noncovalent bonds (π–π interactions, hydrogen bonds, and hydrophobic interactions) helped ZEN to anchor in the binding sites. Finally, a label-free detection method based on gold nanoparticles and Z100 at 0.25 μM was developed for ZEN determination. Excellent linearity was achieved, and the lowest detection limit was 12.5 nM. This rapid and simple method for ZEN analysis has high sensitivity and can be applied for on-site detection of ZEN in animal feeds.
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MICROCHEMICAL JOURNAL
·
2025
·
IF 5.1
Caifeng Zou,Shi Feng,Zongyi Li,Meng Wu,Panhua Li,Yijing Wang,Yanyan Shang,Xiangyang Li
Ochratoxin A (OTA) and zearalenone (ZEN), two ubiquitous mycotoxins accumulating in warm, humid climates, contaminate crops and foods, making accurate on-site detection essential for safeguarding food safety and environmental health. In this study, carbon/niobium carbide nanomaterials (C/NbCNMs) with superior peroxidase-like activity were synthesized through a carbothermal reduction process followed by dilute-acid etching—a treatment that generated abundant active sites and a large specific surface area. Leveraging the nanozyme activity of C/NbCNMs and the specific recognition capability of aptamers, a dual-aptamer colorimetric sensing platform (C/NbCNMs@OTA-Apt and C/NbCNMs@ZEN-Apt) was constructed for the parallel detection of OTA and ZEN. Aptamer conjugation markedly enhanced the catalytic activity of the nanozyme, amplified the chromogenic signal, and enabled detection limits as low as 19 pg mL −1 (0.019 ng mL −1 ) for OTA and 16 pg mL −1 for ZEN, with visual detection limits of 60 and 50 pg mL −1 , respectively. For high-throughput analysis, a dual-channel intelligent model was established to predict toxin concentrations within 10 s via chromatic characteristic analysis. Moreover, the sensing platform exhibited excellent practicality and accuracy in naturally contaminated food samples, with relative standard deviations (RSD) of 3.30 % for OTA and 3.84 % for ZEN. This work offers a low-cost, highly sensitive, portable, and intelligent alternative to conventional mycotoxin detection methods, facilitating rapid on-site screening of OTA, ZEN, and other analogous contaminants.
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