JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION
·
2020
·
IF 2.69
Penghui Wang,Wei Liu,Shuai Liu,Rong Yang,Yajie Pu,Wenjie Zhang,Xiaoxue Wang,Xin Liu,Yanhan Ren,Bo Chi
Here, a novel pH-responsive block copolymer, poly (ethylene glycol)-poly(ε-caprolactone)-poly(L-histidine) (PEG-PCL-PHis), was synthesized and designed for anti-cancer drug delivery with excellent biocompatible, biodegradable, and strong drug loading efficiency. 1H-NMR, IF-IR, and GPC were used to characterize the structure of the PEG-PCL-PHis copolymer. In addition, the morphology, particle size, Zeta potential, and critical micelle concentration (CMC) of different degree of polymerization were determined by transmission electron microscopy (TEM), dynamic light scattering granulometer (DLS), and fluorescence spectrometer, respectively. The strong affinity between the core of micelles and hydrophobic drug was manifested with 15.09% drug loading content and 84.65% entrapment efficiency. In vitro release of DOX from the block copolymer micelle demonstrated, the PEG-PCL-PHis copolymer micelle has stable and durable drug releasing ability accompanied with pH-sensitivity. From the mechanism of cellular uptake the micelles, the pathway of drug release was captured by confocal laser scanning microscope. These experiments demonstrated the safe delivery for anticancer medicine through this novel copolymer. In conclusion, the PEG-PCL-PHis copolymer micelle has great potential to become a safe drug carrier for cancer chemotherapy.
研究领域:
药剂学
·
生物材料
·
肿瘤学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD CHEMISTRY
·
2015
·
IF 3.391
Xing Chen,Yufeng Zou,Minyi Han,Lihua Pan,Tong Xing,Xinglian Xu,Guanghong Zhou
Myosin, a major muscle protein, can be solubilised in a low ionic strength solution containing l -histidine (His). To elucidate which chemical constituents in His are responsible for this solubilisation, we investigated the effects of 5 mM His, imidazole (Imi), l -α-alanine (Ala), 1-methyl- l -histidine (M-his) and l -carnosine (Car) on particle properties of myosin suspensions and conformational characteristics of soluble myosin at low ionic strength (1 mM KCl, pH 7.5). His, Imi and Car, each containing an imidazole ring, were able to induce a myosin suspension, which had small particle size species and high absolute zeta potential, thus increasing the solubility of myosin. His, Imi and Car affected the tertiary structure and decreased the α-helix content of soluble myosin. Therefore, the imidazole ring of His appeared to be the significant chemical constituent in solubilising myosin at low ionic strength solution, presumably by affecting its secondary structure.
研究领域:
生物化学
·
蛋白质科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Analytical Methods
·
2014
·Vol. 7
·期 2
·684-689
·
IF 1.938
Yuan Su,Li Qi,Xiaoyu Mu,Minglin Wang
In this work, L-histidine-stabilized gold nanoclusters were prepared by a green one-pot process and employed as a selective probe for sensing ferric ions. The obtained probe exhibited bluish green fluorescence, and exhibited an excitation maximum at 394 nm and emission maximum at 492 nm. The fluorescence of the probe was obviously quenched by the introduction of ferric ions, hinting that the synthesized probe could be applied for sensing ferric ions. Further, the selectivity of the proposed probe for sensing ferric ions was investigated. The results indicated that the fluorescent probe had favorable sensitivity and selectivity for the detection of ferric ions. Moreover, this probe was further used for the analysis of ferric ions in bean sprouts. It showed that the gold nanocluster based fluorescent probe has great potential for sensing metal ions in plants.
研究领域:
分析化学
·
植物学
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2014
·
IF 4.517
Wei Bian,Fang Wang,Yanli Wei,Li Wang,Qiaoling Liu,Wenjuan Dong,Shaomin Shuang,Martin M.F. Choi
We report a turn-on phosphorescence probe for detection of histidine based on Co 2+ -adsorbed N -acetyl- l -cysteine (NAC) capped Mn: ZnS quantum dots (QDs) which is directly synthesized by the hydrothermal method. The phosphorescence of NAC-Mn: ZnS QDs is effectively quenched by Co 2+ attributing to the adsorption of Co 2+ onto the surface of QDs with a concomitant in suppressing the recombination process of hole and electron of QDs. The phosphorescence of Co 2+ -adsorbed NAC-Mn: ZnS QDs can be recovered by binding of Co 2+ with histidine. The quenching and regeneration of the phosphorescence of NAC-Mn: ZnS QDs have been studied in detail. The as-prepared QDs-based probe is applied to determine histidine with a linear range of 1.25–30 μM and a detection limit of 0.74 μM . The relative standard deviation for eleven repeat detections of 20 μM histidine is 0.65%. Co 2+ -adsorbed NAC-Mn: ZnS QDs show high sensitivity and good selectivity to histidine over other amino acids, metal ions and co-existing substances. The proposed QDs probe has been successfully applied to determination of histidine in human urine samples with good recoveries of 98.5–103%.
研究领域:
生物化学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
DIAMOND AND RELATED MATERIALS
·
2023
·
IF 4.1
Yanan Zhao,Wenjie Liu,Xiangkun Pang,Xiaohui Dai,Feng Gao,Yibin Liu,Qingxiang Wang
Establishing a rapid and sensitive approach for the detecting (NO 2 − ) is of great importance in environmental pollution monitoring and human health protection. Herein, a Fullerol-gold nanocluster@histidine (FLN-AuNCs@His) nanocomposite has been synthesized and utilized as an effective electrochemical sensing material for NO 2 − analysis. The FLN-AuNCs@His nanocomposite and the sensing interface were characterized by Fourier Transform Infrared (FT-IR), scanning electron microscopy (SEM), and electrochemical techniques . Electrochemical experiments show that the FLN-AuNCs@His after electrochemical activation in 0.1 M KCl show significantly enhanced electrocatalysis for the oxidation of NO 2 − . From the physical and electrochemical characterization, the promotion effect is ascribed to the increase of electrocatalytic active sites and the electro-conductivity through the electroactivation. Under the optimal conditions, the catalytic currents showed a good linear relationship with the concentration of NO 2 − in a wide range from 10.0 nM to 10.0 mM with an ultralow detection limit of 1.98 nM (S/N = 3). The sensor is applicable for the analysis of NO 2 − extracted from the soil samples, suggesting its potential application value in environmental sample analysis.
研究领域:
环境科学
·
分析化学
·
纳米材料科学
✓ 已验证
产品关联
DOI
高影响因子
CELLULOSE
·
2020
·Vol. 27
·期 14
·8155-8167
·
IF 4.21
Guo Jianwei,Liu Xiao,Han Miaomiao,Liu Yadong,Ji Shengxiang
Abstract An amphoteric metal adsorbent, poly( N -acryloyl- l -histidine)-modified sawdust (SD- g -P(A-His)), is prepared by reversible addition–fragmentation chain transfer (RAFT) polymerization of N -acryloyl- l -histidine from modified sawdust. SD- g -P(A-His) possess imidazolyl and carboxyl groups and shows outstanding adsorption performance for heavy metals. The adsorption capacities of SD- g -P(A-His) are 241.8 and 95.7 mg/g for Pb(II) and Cu(II), respectively. Ten tested metal ions, e.g. Au(III), Cr(III), Cu(II), Fe(III), Ni(II), Pb(II), Pd(II), Hg(II), Mg(II) and Zn(II), can be effectively removed from the multi-metal solution at pH > 2.7, while Au(III) and Pd(II) can be selectively recycled from the multi-metal solution by lowering the pH to ≤ 1.0. The adsorption kinetics of Pb(II) and Cu(II) onto SD- g -P(A-His) fit well with the pseudo-second order model. SD- g -P(A-His) can treat the water with low concentration of metal ions down to sub-10 μg/L level to meet the Quality Standards for Drinking Water of China. Moreover, SD- g -P(A-His) can be recycled and maintain high adsorption efficiency (91.5%) even after 5 cycles. Graphic abstract
研究领域:
环境科学
·
材料科学
✓ 已验证
DOI
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2014
·
IF 6.451
Xuyue Zheng,Tianming Yao,Ying Zhu,Shuo Shi
In the present study, a new strategy based on Cu 2+ mediated DNA-templated silver nanoclusters (DNA-Ag NCs) was developed, as a label-free, on–off–on fluorescent probe for the detection of l -histidine. Eight synthesized DNA oligonucleotides (D1–D8) were experimentally tested, and D5-Ag NCs was finally selected for l -histidine detection due to its best fluorescent properties. The fluorescence emission of D5-Ag NCs could be quenched by Cu 2+ via electron or energy transfer. Upon addition of l -histidine, the chelation between Cu 2+ and the imidazole group of l -histidine leads to Cu 2+ liberation from D5-Ag NCs, and subsequently results in a dramatic fluorescence enhancement of the probe. The method displayed a good selectivity toward l -histidine over all the other amino acids , with a linear relationship in the range of 0.20–80 μM, and a limit of detection (LOD) of 4.3 nM. The strategy was also successfully applied to detect l -histidine in diluted human urine, exhibiting great opportunities for practical application in biological system.
研究领域:
分析化学
·
生物化学
·
纳米技术
✓ 已验证
DOI
PubMed
高影响因子
SEPARATION AND PURIFICATION TECHNOLOGY
·
2026
·
IF 9.1
Rong Huang,Jian Liu,Yuebing Liu,Lingfang Xie,Dianwen Liu,Shuming Wen,Dongdong He
The efficient flotation separation of sphalerite from pyrite remains a considerable challenge because of their similar floatability in Cu 2+ -activated systems, as well as the poor selectivity and environmental limitations of conventional depressants. In this study, L-histidine (L-HIS), an environmentally benign small organic molecule, was employed as a depressant for sphalerite/pyrite separation under low-alkalinity conditions (pH 8). Its selective depression mechanism toward Cu 2+ -activated pyrite was systematically investigated through flotation tests, multi-scale surface characterization, and theoretical calculations. The results showed that L-HIS selectively depressed pyrite while maintaining sphalerite floatability, producing a recovery difference of approximately 92.51%. This selectivity originated from the different states of Cu species on the two mineral surfaces. For pyrite, adsorbed Cu species were weakly bonded to surface S sites and coordinated strongly with the amino N sites of L-HIS, forming stable Cu N bonds. This weakened the Cu S interaction, promoted the partial removal of the Cu activation layer, and inhibited SEX adsorption. Meanwhile, L-HIS introduced hydrophilic functional groups onto pyrite, markedly reducing its hydrophobicity. In contrast, Cu species on sphalerite mainly existed as lattice-substituted Cu species with stable Cu S bonds. L-HIS showed only weak interaction with sphalerite, so SEX adsorption and surface hydrophobicity were preserved. This study provides a green depressant strategy for low-alkalinity flotation separation of Cu 2+ -activated sulfide minerals.
✓ 已验证
DOI
高影响因子
ANALYTICAL CHEMISTRY
·
2026
·
IF 7.3
Xiujuan Qiao,Rui Han,Guangyan Xu,Peiqi Liang,Yiting Hou,Jiaqi Li,Xiliang Luo
The reliable detection of cortisol in sweat presents a promising, noninvasive approach for stress monitoring. However, in demanding scenarios such as firefighting and mining, rapid sweat evaporation concentrates salts to high levels that destabilize hydration layers and compromise electrochemical biosensors based on conventional zwitterionic peptides. In this work, we developed a salt-tolerant antifouling layer using the polypeptide CPPPP(HE)6, which features alternating histidine and glutamic acid residues, and integrated it with cortisol aptamers to construct a wearable electrochemical biosensor. Compared with conventional zwitterionic peptides of CPPPP(EK)6, the CPPPP(HE)6 peptide was designed by replacing the charge-localized ammonium cation (e.g., lysine-derived NH3+) with a charge-delocalized imidazolium cation from histidine. Combined computational and experimental studies reveal that both systems undergo electron redistribution with Cl– (Cl– is the most abundant anion in sweat). In contrast to the single-site polarization in NH3+, imidazolium+ features multicenter rearrangement coupled with proton migration, which reinforces the hydrogen-bonding network and fosters an exceptionally stable hydration layer under high salinity. As a result, the CPPPP(HE)6 coating exhibits superior fouling resistance compared to CPPPP(EK)6. The resulting antifouling electrochemical biosensor, functionalized with CPPPP(HE)6 and cortisol aptamers, enabled accurate and reliable detection of cortisol in human sweat and high-salinity conditions, with results validated against the standard ELISA method. This work provides a molecular-level design strategy for creating robust antifouling materials, advancing the development of high-fidelity sensors for complex biofluids.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
·
2025
·
IF 2.8
Wanjun Han,Han Liu,Ting Liang,Lanjing Li,Ru Huan,Chunshan Gui
Organic anion transporting polypeptide 1B3 (OATP1B3) is a liver-specific transporter that mediates uptake of various substances from blood into hepatocytes. The transport function of OATP1B3 was shown to be pH-sensitive. As the protonation state of extracellular histidine residues can be affected by the environmental pH, in the present study, the role of 7 extracellular histidine residues in the function and pH sensitivity of OATP1B3 has been examined. Our results showed that H115 had the most significant effect on the function of OATP1B3. The Cryo-EM structure of OATP1B3 indicated that H115 is involved in the binding and release of bicarbonate during a transport cycle. Functional studies on H115 mutants suggested that a hydrogen-bond forming group was preferred over a positively charged group at site 115, indicating that a hydrogen bond is optimum for bicarbonate's binding/release cycle. This may also explain why OATP1B3 showed lower transport function at pH 4.5 than at pH 7.4, as H115 is positively charged at pH 4.5 but neutral at pH 7.4. In addition, the H115A mutation largely compromised the pH sensitivity of OATP1B3, probably due to the loss of its protonation state switching capability. Taken together, H115 plays an important role in the function and pH sensitivity of OATP1B3.
✓ 已验证
DOI
PubMed
高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qingqing Yu,Hui Hong,Yueyue Liu,Abdul Razak Monto,Ruichang Gao,Yulong Bao
The pH buffering capacity is an important functionality of muscle proteins, and muscle foods are susceptible to being oxidized during storage and processing. In order to study the effect of oxidation on the pH buffering capacity of myofibrillar proteins, myofibrils extracted from snakehead fish ( Channa argus ) were oxidized with H 2 O 2 . Results showed that increased oxidation led to loss of free sulfhydryl groups, formation of carbonyl groups, increased surface hydrophobicity , and aggregation of myofibrillar proteins. In addition, there was a significant reduction in the content of histidine in oxidized myofibrillar proteins. The pH buffering capacity of myofibrillar proteins significantly decreased from 3.14 ± 0.03 mM H + /(mL × ΔpH) down to 2.55 ± 0.03 mM H + /(mL × ΔpH) after oxidation with 50 mM H 2 O 2 . Both oxidized myofibrillar proteins and histidine showed a high pH buffering capacity at pH near 5.8, which is the histidine pKa value. Here, we hypothesize that oxidation-induced changes in the pH buffering capacity of myofibrillar proteins were driven by oxidative modification of histidine and structural changes of myofibrillar proteins. The significance of this study to food industry may be the awareness that protein oxidation may affect pH through changes in buffering capacity. And the use of antioxidants, especially those targeting at histidine will be promising in addressing this issue.
研究领域:
食品科学
·
生物化学
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Chengqi Zhang,Ying Wang,Ziyun He,Aoran Wang,Zikuo Li,Xuheng Gao,Haoxue Xia,Wende Liu,Guangfei Tang
Fusarium graminearum is a preeminent fungal pathogen exacting a heavy toll on global crop production. Fludioxonil, a highly effective broad-spectrum fungicide, has been extensively deployed to mitigate the devastation wrought by F. graminearum. Nevertheless, the molecular mechanisms of fludioxonil resistance in this pathogen remain largely enigmatic. In this study, we identify a novel point mutation (L837F) in FgOs1 (osmosensor-like histidine kinase), which is located in the HIS_KIN (histidine kinase) domain. Biological assays revealed that the FgOs1L837F mutation reduced the ability of F. graminearum to adapt to low-temperature stress, osmotic stress, and metal ion stress and also decreased its pathogenicity. Additionally, this mutation did not confer cross-resistance to the fungicides phenamacril, tebuconazole, and carbendazim. To facilitate field monitoring, we employed allele-specific PCR (AS-PCR) to detect FgOs1L837F point mutation in F. graminearum. This study offers crucial support for fungal disease control and crop protection.
研究领域:
植物病理学
·
农业微生物学
·
分子植物病理学
·
真菌遗传学
·
农药抗性机制
·
作物保护
·
信号转导
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）