CHEMICAL ENGINEERING JOURNAL
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2026
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IF 12.5
Xinru Yang,Yuxin Zhang,Chunhui Zhang,Fuyu Li,Cuiping Wang,Zhiyuan Zhang,Kai Yu,Hongwen Sun,Baoshan Xing
Waste-derived zeolite efficiently removes Cd across various pH conditions. • pH conditions drive the shift in Cd removal mechanisms on zeolite. • Hydroxyl groups in soil dissolved organic matter enhance Cd fixation on zeolite. • Sustainable strategy integrates waste reuse with remediation. Soil dissolved organic matter (DOM), which is ubiquitously released during remediation of heavy metal-contaminated soil-water systems, exerts significant yet underexplored influence on heavy metal fate. Here, we systematically investigated the effect of soil DOM on cadmium (Cd) adsorption using industrial waste-synthesized zeolites, which showed high adsorption efficiency across pH 3.0–7.0. More importantly, adsorption-desorption experiments confirmed that soil DOM selectively enhanced Cd immobilization on zeolites through its functional groups. By integrating EEM-PARAFAC, FT-ICR-MS, and 2D-FTIR-COS analyses, we further identified the dominant soil DOM components and specific functional groups responsible for the immobilization. In soil DOM-free systems, Cd adsorption was primarily governed by Si/Al O bonds, whereas in soil DOM-amended systems, Cd retention was jointly controlled by zeolite surface sites and DOM-derived oxygen-containing functional groups, forming a stable zeolite-soil DOM-Cd ternary system, in which hydroxyl groups ( OH) played a dominant role in promoting Cd immobilization. This work provides new molecular-level insights into the critical role of soil DOM in enhancing heavy metal immobilization and offers a scientific basis for developing effective remediation strategies using waste-derived zeolites for treating Cd contaminated wastewater. Download: Download high-res image (145KB) Download: Download full-size image
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高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2021
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IF 8.128
Yuxiao Feng,Hongtong Hu,Zichen Wang,Yingjie Du,Le Zhong,Chenxi Zhang,Yanjun Jiang,Shiru Jia,Jiandong Cui
Metal-organic frameworks (MOFs) have been emerged as a promising support for immobilizing enzymes owing to the tunable porosity, high surface area, and structural diversity. However, most of these possess nanometer size and small pores, which are difficult to recover them from the reaction medium and present low immobilization efficiency and protein loading capacity, and high substrate diffusion limitations. Herein, a novel magnetic amino-functionalized zeolitic imidazolate framework-8 (ZIF-8) with 3D highly ordered macroporous structure was synthesized using the assembled polystyrene (PS) nanosphere monoliths as a template. Subsequently, catalase (CAT) molecules were immobilized on the surface of macroporous magnetic ZIF-8 and inside the macropores by precipitation, covalent binding and cross-linking. The resultant immobilized CAT showed high immobilization efficiency (58%) and protein loading capacity (29%), leading to 500% higher activity than the immobilized CAT on ZIF-8 (CAT/ZIF-8). Meanwhile, the immobilized CAT could be easily recovered with a magnet without obvious activity loss. The traditional CAT/ZIF-8 lost its activity after 6 cycles, whereas, the immobilized CAT retained 90% activity of its initial activity after reusing for 8 cycles, indicating excellent reusability. In conclusion, this study provides a facile and efficient approach to immobilize enzymes on/in MOFs with enhanced activity and excellent recyclability.
研究领域:
生物化学
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材料科学
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PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2020
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IF 6.551
Ting Wang,Tianwei Qian,Dongye Zhao,Xiaona Liu,Qingwei Ding
Radioactive pertechnetate (TcO4 − ) has been detected in nuclear waste affected soil and groundwater, posing significant effect on human health and the environment. Yet, cost-effective remediation of Tc-contaminated soil and groundwater remains challenging. To address this critical technology need, we prepared a class of pyrite (FeS 2 ) particles for effective immobilization of pertechnetate. Using perrhenate (ReO 4 − ) as a non-radioactive surrogate of TcO 4 − , we tested the immobilization effectiveness of the material through batch kinetic experiments, and evaluated the remobilization potential of immobilized Re under anoxic (sealed from air) and oxic (exposed to air) conditions and in the presence of humic acid (HA), EDTA, nitrate, and a Chinese loess soil. The results showed that more acidic pH gave faster Re(VII) removal due to more abundant electron sources (Fe 2+ and S 2 2− ). X-ray diffraction (XRD) and/or X-ray photoelectron spectroscopy (XPS) analyses confirmed formation of ReO 2 /ReS 2 as the major reduction products. The immobilized Re remained highly stable when aged for 360 days under anoxic conditions at different influence factors. Yet, the immobilized Re was vulnerable to oxygen oxidation, and about 78% of Re was remobilized after 40 days of exposure to air regardless of the initial pH (3.5–9.0) due to excessive pyrite oxidation and the associated pH drop (~2). HA at 120 mg/L inhibited Re remobilization under oxic conditions, which lowered the Re remobilization by ~21% after 40 days of oxic aging. The presence of EDTA facilitated dissolution of Fe but inhibited the dissolution of Re under oxic conditions. Nitrate showed negligible effect on Re remobilization. The presence of a Chinese loess soil effectively inhibited Re remobilization under both oxic and anoxic conditions, lowering the leachable Re by ~32% under oxic conditions. The findings may guide engineered application of pyrite particles as a long-lasting reducing material for immobilization pertechnetate or similar redox-active contaminants in soil and water.
研究领域:
环境科学
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放射性废物处理
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PubMed
高影响因子
SCIENCE OF THE TOTAL ENVIRONMENT
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2018
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IF 4.61
Jiajiang Lin,Mengqiang Sun,Binglin Su,Gary Owens,Zuliang Chen
While phytogenic nanomaterials have been successfully used to remove heavy metals in wastewater, the potential to successfully use such materials to immobilize heavy metals in soils is still unclear. In this study, phytogenic iron oxide nanoparticles (PION) were used to immobilize cadmium (Cd) in six soils. Amendment with PION effectively immobilized Cd, with a concomitant increase in the concentrations of iron oxides, soil pH and dissolved organic carbon (DOC) under both oxic and anoxic conditions . However, observed changes in soil properties and Cd fractions were different under oxic and anoxic conditions. After PION application, the exchangeable Cd fraction decreased by up to 91 and 69%, while the carbonate bound Cd fraction decreased by up to 61 and 75%, under oxic and anoxic conditions, respectively. Pearson correlation analysis revealed that under both oxic and anoxic conditions, Cd fractions were significantly and positively correlated with free iron oxide content and pH, where free iron oxide content was positively correlated with amorphous iron oxide, DOC and pH. The Cd immobilization mechanisms potentially involved either (1) formation of insoluble hydroxides at elevated pH; (2) participation of biomolecules released from PION in ligand complexation with Cd and (3) co-precipitated of Cd during the formation of iron oxides. This study provided new insights into the potential effects of PION applications for practical Cd immobilization in contaminated soils.
研究领域:
环境科学
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土壤学
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PubMed
高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2019
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IF 3.371
Yanjun Jiang,Huan Liu,Lihui Wang,Liya Zhou,Zhihong Huang,Li Ma,Ying He,Lujing Shi,Jing Gao
Hydrophobic virus-like organosilica nanoparticles (VOSNs) with a spherical core formed by mesoporous organosilica surrounded by epitaxial perpendicular nanotubes were successfully synthesized for the first time. These hydrophobic VOSNs were used for the immobilization of the lipase B from Candida antarctica (CALB) via covalent binding method, where glutaraldehyde was used as a spacer to form covalent bonds connect between the enzyme and support. Under optimized immobilization conditions, the specific hydrolytic activity which determined via the hydrolysis of p-NPP was 23200 U/g support , and the maximum CALB loading was 115.6 mg/g support . Compared to virus-like silica nanoparticles (VSNs), the hydrophobic VOSNs protected the CALB active conformation from a hazardous external environment due to stronger hydrophobic interactions between the VOSNs and CALB molecule. Thus, compared with CALB@VSNs and free lipase, CALB@VOSNs exhibited better pH, thermal stability, organic solvents tolerance and storage stability. The CALB@VOSNs was used to catalyze the esterification reaction of levulinic acid (LA) and n-lauryl alcohol, which was optimized by a response surface method. Under the optimal conditions (temperature, 51.2 °C; LA: n -lauryl alcohol, 1:11.5; time, 22.6 h), the CALB@VOSNs still retained 75.7% of the LA conversion, whereas the N435 only retained 29.8% of the conversion after 9 cycles. These results showed that hydrophobicity VOSNs could be an efficient support to prepare immobilized enzymes as well as to improve the properties of enzymes.
研究领域:
生物化学
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材料科学
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酶工程
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高影响因子
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2011
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IF 2.072
D. Tian,X. H. Chu,D. H. Yu,Y. Z. Yue,P. Zhao,X.L. Sun,W. L. Liu
Polymethyl methacrylate (PMMA) brushes with a quantitative molecular weight (Mw) and low polydispersity index are synthesized via atom transfer radical polymerization and grafted onto the surfaces of hydroxyapatite (HA) that were modified with γ-methacryloxypropyl trimethoxysilane (MPTS). The structure and morphology of MPTS surface modified and furthermore PMMA grafted HA was mainly investigated by means of infrared spectrophotometry, X-ray diffraction, thermal gravimetric analysis, scanning electron microscope, and differential scanning calorimetry studies. It is found that polymer brushes are covalently attached to the HA surface under controlled Mw of PMMA segments without any structural deformation on the HA phase and exhibit grafting abilities for tuning properties of the composites.
研究领域:
材料科学
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生物医学工程
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高影响因子
BIOCHEMICAL ENGINEERING JOURNAL
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2011
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IF 2.692
Pixiang Wang,Xiangyu Gong,Erzheng Su,Jingli Xie,Dongzhi Wei
A facile pretreatment method was developed to obtain high specific activity of PGA from recombinant E. coli cells for preparing immobilized enzyme with high activity, which coupled selective extraction of PGA with butyl acetate and adsorption of butyl acetate with active carbon. Butyl acetate (5%, v/v) led to a 92.0% release of PGA, the specific activity of which in the extracting solution was twice that obtained by sonication. The negative effect on PGA immobilization due to residual butyl acetate in the extracting solution was removed by adding active carbon (8%, w/v). The final PGA solution meets requirement for industrial immobilization while eliminating the high cost of a traditional purification process. This pretreatment method developed in this work is simple, highly performing and cost-effective. It has been employed in a 10 tons/year immobilized PGA production line.
研究领域:
生物化学
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酶工程
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高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2012
·Vol. 166
·期 6
·1433-1441
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IF 1.943
Yu Yuanyuan,Yuan Jiugang,Wang Qiang,Fan Xuerong,Wang Ping
The covalent immobilization of a commercial preparation of cellulase on a reversibly soluble–insoluble enteric polymer Eudragit S-100 by carbodiimide coupling was carried out. The characteristics of covalent Eudragit cellulase were evaluated using Fourier transform infrared (FTIR) spectra, circular dichroism (CD) spectra, and fluorescence spectra. FTIR, CD, and fluorescence measurements also revealed that the cellulases were covalently bonded to the supports. Covalent Eudragit cellulase had binding efficiency of 81.08% which was higher than the noncovalent Eudragit cellulase 56.83%. The relative activity of the native cellulase and covalent Eudragit cellulase increased and reached the maximum (at pH 5.0, 50°C) and then decreased with further increases in pH and temperature. The covalent Eudragit cellulase shows higher stability especially at higher pH and temperature. The K
m value of covalent Eudragit cellulase (4.78 g·L −1 ) was decreased compared to that of the native cellulase (2.89 g·L −1 ). The affinity of the cellulase to its substrate was increased when it was immobilized on Eudragit S-100.
研究领域:
生物化学
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酶工程
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PubMed
高影响因子
Advances in Materials Science and Engineering
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2018
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IF 1.372
Baoguo Ma,Ting Zhang,Hongbo Tan,Xiaohai Liu,Junpeng Mei,Wenbin Jiang,Huahui Qi,Benqing Gu
Utilization of sea sands and coral aggregate for concrete in ocean construction is increasingly attracting the attention all over the world. However, the potential risk of steel corrosion resulting from chloride in these raw materials was one of the most concerned problems. To take this risk into account, chloride transporting to the surface of steel should be hindered. The formation of Friedel’s salt in hydration process is widely accepted as an effective manner for this hindrance. In this study, an attempt to hasten the formation of Friedel’s salt by adding triisopropanolamine (TIPA) was done in the cement-fly ash system, with intention to chemical bind chloride, and the chloride-binding capacity at 60 d age was examined. The results show that TIPA can enhance the chloride-binding capacity of cement-fly ash paste at 60 d age, and the reason is that the formation of Friedel’s salt can be accelerated with addition of TIPA. The mechanism behind is revealed as follows: on the one hand, the accelerated cement hydration provides more amount of calcium hydroxide to induce the pozzolanic reaction of fly ash, which can hasten the dissolution of aluminum into liquid phase; on the other hand, TIPA can directly hasten the dissolution of aluminum in fly ash, offering more amounts of aluminum in liquid phase. In this case, the aluminum/sulfate (Al/S) ratio was obviously increased, benefiting the formation of Friedel’s salt in hydration products. Such results would expect to provide useful experience to promote the chloride-binding capacity of cement-fly ash system.
研究领域:
土木工程
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材料科学
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JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY
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2012
·Vol. 62
·期 3
·424-431
·
IF 1.632
Liu W. L.,Wang L.,Zhang L. D.,Xu W. H.,Chen S. H.,Wang X. Q.,Duan X. L.
Abstract The organic–inorganic nanocomposite films were fabricated by grafting polystyrene (PS) onto the vinyltriethoxysilane (VTEOS) modified titanium dioxide nanopowders using free radical polymerization. The composition of the surfaces and the structure for the PS grafted titania (PS-g-TiO 2 ) were examined by infrared spectroscopy, X-ray photoelectron spectroscopy and thermogravimetric analysis, and the rough surface was confirmed by the evaluation of the morphological characteristics of the coating using hybrid particles. The wetting properties of the VTEOS modified titania and PS-g-TiO 2 films were investigated, which show the maximum static water contact angles of 160° and 154°, and minimum sliding angles of 3° and 4°, respectively. Graphical Abstract
研究领域:
材料科学
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纳米技术
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表面化学
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高影响因子
ACS Applied Bio Materials
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2018
·
IF 0
Jun Xing,Qiyou Wang,Tianrui He,Zhengnan Zhou,Dafu Chen,Xin Yi,Zhengao Wang,Renxian Wang,Guoxin Tan,Peng Yu,Chengyun Ning
The hemodialysis therapy is the primary treatment for the end-stage renal disease (ESRD) patients to extend their lives. To reduce the pathogenic infection during hemodialysis treatment, mussel-inspired polydopamine (PDA) was self-polymerized onto poly(ether sulfone) (PES) membranes to immobilize copper ions, because PDA coating could firmly adhere to the PES membranes and immobilize antimicrobial metal ions with its functional groups. The immobilized copper ions through PDA can penetrate deeply into the inner surface of the pores of PES membrane. Water permeability test further indicates PDA coating can obviously enhance the hydrophilicity of PES membranes. And copper ion immobilized PES membranes showed effective antibacterial activity with a relative safe release amount of copper ions (below 1 ppm). Furthermore, hemolysis test results revealed that PDA-assisted antibacterial PES membranes can relieve red blood cells damage compared to the pure PES membranes. To sum up, PES hemodialysis membranes obtained the antibacterial ability by the PDA-assisted copper ion immobilization, which can reduce the abuse of antibiotics and is beneficial to the patient suffering from kidney disease.
研究领域:
材料科学
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生物医学工程
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肾病学
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PubMed
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2015
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IF 2.729
Berlin Zang,Jun Ren,Li Xu,Lingyun Jia
Immobilization of affinity ligands on supporting matrices is a key step for the preparation of affinity chromatography resins, and an efficient coupling strategy can significantly improve the validity and cost of the affinity system, especially for systems that employ expensive recombinant proteins or antibodies as affinity ligands. This study described a simple method for obtaining site-specific immobilization of protein A (the ligand) via aldehyde-hydrazide conjugation and its application in antibody purification via protein A chromatography. An aldehyde group was generated at the N-terminus of protein A in vivo by co-expressing a formylglycine-generating enzyme (FGE) and recombinant protein A containing a FGE recognizing sequence (aldehyde tag) in Escherichia coli . The resulting aldehyde allowed direct immobilization of protein A onto the hydrazide-modified agarose matrices under mild condition. We found that 100 mM aniline was most effective for catalyzing the coupling reaction, and the recombinant protein A could be coupled with high selectivity, directly from a crude cell extract. The site-specific immobilized protein A showed good capacity for antibody purification. The specificity of the aldehyde-hydrazide reaction not only allowed site-specific immobilization of affinity ligands, but also improved the cost of the process by employing unpurified ligands, a method that might be of great use to industrial applications.
研究领域:
生物化学
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分子生物学
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蛋白质工程
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PubMed
高影响因子
危险品化学品经营许可证（带存储）