ELECTROCHIMICA ACTA
·
2024
·
IF 5.5
Li Tan,Wenyi Zhang,Ziqi Lian,Yu Wang,Wenjing Gao,Tao Zeng,Yun Chen,Meng Yu,Benlin Hong,Rongkai Ye,Ying Ma,Yuchan Liu,Jianqiang Hu,Jiaju Mo,Aiqing Li
ABSTRACT MicroRNAs (miRNAs) are important biomarkers for tracking occurrence and development of many diseases, therefore, the reliable, sensitive, fast and accurate detection of miRNAs plays an extremely critical role in clinic diagnosis and treatment of early stage diseases. Herein, we developed ultrasensitive electrochemiluminescence (ECL) biosensor constructed by luminol-diazonium ion functionalized Au/MXene nanocomposites for early stage detection of kidney disease in human. The synergistic effect of MXene and Au nanoparticles significantly enhanced the ECL emission of luminol up to 6.9-fold due to their shortened electron transfer distance and excellent catalytic performance. The loading efficiency of luminol on MXene could be as high as ∼7%, enabling the enrichment of luminol on MXene nanosheets. The enhanced ECL signal and excessive loading of luminol allowed the Au-MXene-luminol as an excellent ECL labeling agent, which was further developed for the determination of miRNA-377 according to the sandwich-like assay. The as-developed miRNA-377 biosensor had ultrahigh sensitivity, wide linear range (from ∼50 aM to ∼1 nM), low limit of detection (∼13.2 aM) and high selectivity (∼82.6%) for highly homologous miRNA with mismatched single-bases. The ultrasensitive ECL biosensor was successfully applied to determine miRNA-377 in the serum samples of early stage kidney patients, which were well consistent with the clinic standard of quantitative real-time polymerase chain reaction method. The ultrasensitive ECL-labeling biosensor with high selectivity is very promising for universally screening any kinds of biomarkers with corresponding specific targets.
研究领域:
生物传感器
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纳米技术
·
电化学
·
医学诊断
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临床化学
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产品关联
DOI
高影响因子
RARE METALS
·
2026
·Vol. 45
·期 5
·e70269
·
IF 12.1
Yu Cui,Liuyi Huang,Jingwen Zhao,Jiyang Liu,Wei Hu
en This link goes to a English section zh This link goes to a Chinese section The precise quantification of cytokines is crucial for monitoring disease onset and progression. Herein, we developed a synergistic signal amplification approach by utilizing nanoreactor-encapsulated CeO 2 nanozymes, which were in situ electrodeposited within the mesoporous silica nanochannel film (SNF) grown on indium tin oxide (ITO) electrodes (CeO 2 @SNF/ITO). Notably, CeO 2 @SNF/ITO possesses bifunctional enzyme-like activities, enabling efficient regulation of two co-reactants, including endogenous dissolved oxygen (DO) and exogenous hydrogen peroxide (H 2 O 2 ). Under neutral pH conditions, electrochemical reduction of the encapsulated CeO 2 leads to the transformation of Ce(IV) into Ce(III). The resulting Ce(III) species not only facilitates the oxygen reduction reaction through its oxidase-like activity but also catalyzes H 2 O 2 decomposition via peroxidase-like behavior. This process leads to the abundant generation of reactive oxygen species (ROS), which can effectively oxidize luminol anions, thereby significantly enhancing the cathodic electrochemiluminescence (ECL) signal. The reversible Ce(III)/Ce(IV) redox cycle also confers outstanding electrode reusability. As a proof of concept, an immunosensor for selective detection of the cytokine interleukin 6 (IL-6) was constructed by modifying the outer surface of the SNF. The proposed immunosensor exhibits high sensitivity for IL-6 detection, achieving a remarkably low detection limit of 2.5 fg mL −1 over a concentration range from 10 fg mL −1 to 10 ng mL −1 . This work offers a promising avenue for expanding the functional scope of SNF-confined nanozymes in advanced ECL systems for immunosensing. 摘要 en This link goes to a English section zh This link goes to a Chinese section 细胞因子的精确定量对监测疾病的发生与进展至关重要。在此,我们利用纳米反应器封装CeO 2 纳米酶,开发了一种协同的信号放大方法。通过原位电沉积技术将CeO 2 纳米酶封装于生长在氧化铟锡(ITO)电极上的介孔二氧化硅纳米通道薄膜(SNF)内,制备CeO 2 @SNF/ITO。值得注意的是,CeO 2 @SNF/ITO具有双重类酶活性,能够高效调控内源性溶解氧(DO)和外源性过氧化氢(H 2 O 2 )两种共反应物。在中性pH条件下,通过电化学还原可以使封装的CeO 2 中的Ce(IV)转化为Ce(III)。生成的Ce(III)不仅可以凭借其类氧化酶活性促进氧还原反应,还能通过类过氧化物酶行为催化H 2 O 2 分解。上述两个过程可积累大量的活性氧物种(ROS),有效氧化鲁米诺阴离子(LH − ),从而显著增强阴极电化学发光(ECL)。可逆的Ce(III)/Ce(IV)氧化还原循环还赋予了电极出色的可重复使用性。作为概念验证,通过在SNF外表面修饰抗体,构建了用于细胞因子白细胞介素-6(IL-6)检测的免疫传感器。该传感器能够实现对IL-6的超灵敏检测,检测线性范围为10 fg mL −1 至10 ng mL −1 ,检测限低至2.5 fg mL −1 。
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DOI
高影响因子
JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2025
·
IF 9.7
Tao Liao,Jiaqiang Huang,Xukang Sun,María Belén Camarada,Xuemei He,Pin Zhang,Xiaoning Liao
The luminol–H 2 O electrochemiluminescence (ECL) system has received increasing attention in recent years owing to its ability to overcome the intrinsic drawbacks of conventional co-reactants, specifically the self-decomposition of hydrogen peroxide and low solubility of dissolved oxygen. However, this system still suffers from a high triggering potential, which leads to excessive oxygen (O 2 ) bubble accumulation at the electrode surface and unstable ECL signals. Herein, we proposed a new ECL enhancement strategy for the luminol–H 2 O system using multiwalled carbon nanotube (MWCNTs)-functionalized bimetallic cobalt–zinc zeolitic imidazolate framework (CoZn-ZIF) as a co-reactant accelerator. Benefiting from the synergistic interaction, the MWCNTs-modified CoZn-ZIF (CoZn-ZIF@MWCNTs) demonstrated superior oxygen evolution reaction activity at a low potential (0.6 V vs. Ag/AgCl), leading to stable and high-intensity ECL signals through efficient catalytic conversion of H 2 O molecules into hydroxyl radicals (·OH) and superoxide radicals (O 2 ·− ). By integrating molecular imprinting technology, a novel ECL sensor was developed for the detection of ochratoxin A, achieving a limit of detection of 0.023 nM (S/N = 3) and wide linear range (0.1–10,000 nM). In real sample analysis, the sensor showed satisfactory recoveries of 99.30 %–110.30 %, demonstrating high accuracy. This work not only establishes a new pathway for enhancing luminol ECL performance but also provides innovative insights for advancing ECL-based sensing technologies in food safety and environmental monitoring.
研究领域:
分析化学
·
电分析化学
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生物传感器
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食品安全监测
·
环境监测
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产品关联
DOI
PubMed
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Lei Hu,Junkai Hao,Ruhui Shang,Huitian Zhi,Xujie Wang,Yingchao Yang,Hanning Song,Chenxi Li,Juan Yu,Zhengquan Yan
Background Environmental safety and protection is accordingly one of the hottest issues to be concerned nowadays. Among all the chemicals to be monitored urgently, aminophenol and luminol are both quite hazardous but widely utilized in the fields of chemical industry, medicine, scientific research, chemical sensing, and so on. Although some reported ways could detect them satisfactorily, few reports could be found for portable visual monitoring and degradation of aminophenol and luminol, using only one sensor to judge the degradation degree as well. Results Fe-doped-Ce oxide modified chitosan fiber (Fe–Ce ox@CF) was developed by microwave-assisted hydrothermal method. By virtue of the excellent physical and chemical performance of carboxylated chitosan and its synergistic effect, Fe–Ce ox@CF possessed prominent oxidase-mimic activity to catalyze over 94 % luminescent luminol into dicarboxylic acid at room temperature. When utilized to accelerate the oxidization of ABTS, K m / V max were 0.0403 mM/2.327 × 10 −8 M s −1 . Especially, ultra-trace m -aminophenol ( m -AP) could exclusively alter the color of Fe–Ce ox@CF-ABTS from green to purple-red with remarkable visibility. Under the optimized conditions, there exhibited an excellent ratiometric colorimetric linear relationship between lg( A 515 / A 730 )/lg( A 515 / A 415 ) and c m -AP , and a fluorescent one between ΔF 427 and c luminol with detection limits (LOD) of 2.0 × 10 −9 M and 5.6 × 10 −9 M respectively. Especially, the red-green-blue (RGB) values got by a common smartphone also possessed good linear relationships with LODs of 1.7 × 10 −8 M and 1.34 × 10 −8 M. Significance This work represents the first oxidase-mimic based sensing system for potable and quantitative degradation of luminol and visual detection of m -aminophenol as well with the help of smartphones. It will also shed promising light on modified natural carbohydrate polymer-based sensors by non-destructive self-assembly and further application in practices.
研究领域:
环境安全
·
化学传感
·
材料科学
·
分析化学
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产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2025
·
IF 10.5
Xiaobo Zhang,Xiuwen Wang,Longjiao Zhu,Jinyan Zhu,Qiuyue Zheng,Jingli Yuan,Wentao Xu,Jijuan Cao
Salmonella typhimurium ( S. typhimurium ), as common and highly pathogenic foodborne pathogen, poses a significant risk to public safety worldwide. The development of highly sensitive, rapid and on-site method for S. typhimurium analysis is urgently needed to prevent bacterial infections. Herein, we introduced a CRISPR/Cas12a-driven electrochemiluminescence (ECL) sensor based on luminol/O 2 binary systems for S. typhimurium detection, employing ultrafine Pt nanoclusters-anchored 2D delaminated-MXenes (Pt NCs/D-MXenes) as the co-reactant accelerator. The ultrathin D-MXenes support regulates the size and dispersibility of Pt NCs and facilitates the full exposure of active sites, and synergistic interactions between D-MXenes and Pt NCs improves electrocatalytic properties toward the reduction of O 2 , which promotes the generation of ROS for boosting ECL emission. Using target responsive-regulated CRISPR/Cas12a system, the ECL sensor for S. typhimurium showed a broad concentration range from 10 1 to 10 6 CFU/mL and limit of detection of 6 CFU/mL, with satisfactory recoveries in spiked-actual samples.
研究领域:
生物技术
·
食品安全
·
分析化学
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产品关联
DOI
PubMed
高影响因子
NEW JOURNAL OF CHEMISTRY
·
2025
·
IF 2.5
Xiao-Jian Li,Xinli Lu,Yunxia Wang,Zhanquan Zhang,Yongfa Zhang
This study successfully developed a highly sensitive electrochemiluminescence (ECL) sensor based on the ECL resonance energy transfer mechanism for detecting the key biomarker of acute myocardial infarction, heart–type fatty acid binding protein (h–FABP). Through the meticulous design of the Ag/Cu2O/Ti3C2 ternary nanocomposite material, this material demonstrated significant catalase–like catalytic activity, enabling the efficient decomposition of H2O2 to generate more superoxide radicals (O₂•⁻), thereby significantly enhancing the ECL signal of the luminol–H2O2 system. Notably, AgS quantum dots (AgS QDs), due to broad–spectrum ultraviolet absorption overlapping with the ECL emission spectrum of luminol, triggered an efficient energy resonance transfer effect, resulting in the decrease of ECL intensity. NH2–MIL–101(Fe) was utilized to couple with AgS QDs (AgS QDs@NH2–MIL–101(Fe)), which can further enhance the quenching efficiency. Based on this signal–off strategy, an ultrasensitive detection platform for h–FABP was successfully constructed. Experimental results indicated that the immunosensor exhibited an excellent response relationship within a wide linear range from 1.00 fg mL–1 to 100 ng mL–1, and a low detection limit reached 0.36 fg mL–1 (S/N = 3). This study provides an innovative methodological approach for ultra–trace detection of myocardial injury markers and demonstrates significant application potential in early clinical diagnosis.
研究领域:
分析化学
·
生物传感器
·
纳米技术
·
生物化学
·
临床诊断
·
心血管研究
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Wei Zhang,Yan Jiang,Fengna Xi
Accurate detection of placental growth factor (PlGF) is crucial for early diagnosis, risk assessment, and clinical management of preeclampsia. Herein, we present an electrochemiluminescence (ECL) aptasensor targeting PlGF with high sensitivity, in which Ni(OH) 2 –CeO 2 composite nanocatalyst was confined in the vertically-order nanochanels to facilitate the ECL of luminol-dissolved oxygen (DO). These Ni(OH) 2 –CeO 2 composite nanocatalyst exhibited good catalytic performance towards oxygen reduction during electrochemical cathodic scanning and could catalyze the electrochemical oxidation of luminol anions. Compared to catalysts directly electrodeposited on indium tin oxide (ITO) electrode, the nanocatalyst confined within the ultra-small VMSF nanochannels demonstrated stronger ECL enhancement effects and higher stability. Additionally, the composite of Ni(OH) 2 and CeO 2 exhibited remarkable synergistic catalytic performance. By immobilizing PlGF recognition aptamer (Apt) on the VMSF surface, an aptasensor was constructed for the highly sensitive analysis of PlGF. Upon the capture of target PlGF (Ag) by recognition aptamer, the formed Apt-Ag complex blocking the nanochannel causes the hindered diffusion of luminol, ultimately leading to a decreased ECL signal for the quantitation of PlGF. Convenient, and sensitive detection of PlGF was achieved by the developed ECL aptasensor with wide linear range (10 fg/mL ∼ 10 ng/mL) and low limit of detection (LOD 1.2 fg/mL). Additionally, analysis of real human serum was realized. The aptasensor demonstrated good selectivity, anti-interference, reproducibility, and long-term storage stability.
研究领域:
生物分析化学
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生物传感器
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纳米技术
·
电化学
·
临床诊断
·
母胎医学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2020
·
IF 7.1
Shanshan Wang,Minmin Wang,Chuanping Li,Haijuan Li,Chunhua Ge,Xiangdong Zhang,Yongdong Jin
Due to their high catalytic effect on the electrochemiluminescence (ECL) of luminol, noble metal nanoparticles (NPs) have been widely used in luminol-based ECL immunosensors. However the aggregation of NPs often resulted in the decrease of ECL signal and affected the stability and durability of the sensor. In this study, a microflower-like structure made of ultrathin Co/Ni-based metal-organic frameworks (MOF) nanosheets were used as a platform for luminol-functionalized AgNPs to construct an immunosensor for tumor marker alpha-fetoprotein. The nanosheet and microflower-like assembly structure of the Co/Ni-MOF greatly improved the ECL performance of the luminol-AgNPs system, attributed to their large surface area, resistance to particle agglomeration and the high catalytic activity of the Co/Ni-MOF. The ultrathin Co/Ni-MOF was full of atomically dispersed cobalt ions and nickel ions, which can catalyze the ECL reaction of luminol and H 2 O 2 . The as-fabricated immunosensor has a good sensitivity with detection limit of 0.417 pg mL −1 (S/N=3), and shows satisfactory performance in practical applications.
研究领域:
生物医学工程
·
分析化学
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DOI
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2018
·
IF 3.235
Yunxia Hu,Chaomin Chen,Yuan Liu,Sui Wang,Zhiyong Guo,Yufang Hu
In the present work, a dual signals electrochemiluminescence (ECL) ratiometric strategy was designed based on the synergic effect of the catalysis effect and ECL resonance energy transfer . It was found that CdSe quantum dots (CdSe QDs) can catalyze the oxidation of luminol to promote the signal intensity of luminol at 0.45 V. At the same time, a stronger cathodic ECL peak at −0.75 V from CdSe QDs was observed, which could be attributed to the resonance energy transfer between luminol as a donor and CdSe QDs as an acceptor. Moreover both signals from two different potentials increased with the increase hydrogen peroxide concentration. On the basis of the above results, an enzyme-free ECL sensor was fabricated by immobilization of coating CdSe QDs on Au-graphite oxide (GO-Au) composites modified glassy carbon electrode (GCE), and luminol solution as probe solution, which was used to detect rapidly and sensitively hydrogen peroxide in the range of 0.5–500 μM with a detection limit of 0.5 μM based on the ration of two signals. The sensor exhibited good reproducibility and sensitivity, suggesting that the simple method will be promising in the detection of active oxygen in environmental samples.
研究领域:
分析化学
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生物传感
·
环境科学
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产品关联
DOI
高影响因子
DYES AND PIGMENTS
·
2019
·
IF 4.018
Wenjing Qi,Maoyu Zhao,Yuling Fu,Hongkun He,Xue Tian,Di Wu,Yan Zhang,Ping Ping Hu
Lanthanide coordination nanoparticles terbium(III) (Tb 3+ )-based fluorescent nanoparticles (luminol-Tb NPs) are synthesized via aggregation-induced fluorescence strategy. Fluorescence of luminol-Tb NPs is enhanced nearly 40 times compared with luminol, which is the effect of aggregation-induced fluorescence by Tb 3+ . Luminol-Tb NPs with high fluorescence is utilized in fluorescent “turn-off” detection of uric acid (UA) within 1 min from 0.1 to 50 μM with the limit of detection (LOD) of 28 nM. The fluorescence quenching effect may be ascribed to photoinduced electron transfer occurs from luminol-Tb NPs to UA. This fluorescent method exhibits high selectivity in UA detection towards metal ions, glucose, ascorbic acid , dopamine, creatinine, urea and cysteine. When it is also applied in detection of UA in serum samples, 5 μM, 10 μM and 30 μM UA are respectively added to serum samples and recoveries range from 97.5% to 101.3% in serum samples. It demonstrates the practical value of luminol-Tb NPs for UA detection in serum.
研究领域:
生物化学
·
分析化学
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产品关联
DOI
高影响因子
MICROCHIMICA ACTA
·
2014
·Vol. 181
·期 11
·1223-1230
·
IF 3.719
Wang Ke,Wei Xiuhua,Tu Yifeng
This paper reports on the synthesis of AuAg and PtAg alloy nanoclusters (NCs) and their enhancement effect on the electrochemiluminescence (ECL) of luminol. The conditions of synthesis were optimized, and the structure and properties of the NCs were characterized by X-ray diffraction, transmission electron microscopy, electrochemistry, and optical spectroscopy. The NCs are found to intensify (by up to 20 times) the ECL of luminol in solution of pH 8.5. This finding can largely extend the useful pH range of the ECL of luminol. The enhanced ECL is strongly affected by oxygen and hydrogen peroxide, and the mechanism of enhancement is attributed to the accelerated production of reactive oxygen species. The enhanced ECL is also affected by phenolic artificial estrogens, and this was used for their determination with detection limits as low as 700 pg L− 1 (with AuAg) and 1.6 ng L− 1 (with PtAg). The method was applied to the determination of such estrogens in egg samples using diethylstilbestrol as a reference substance.
The alloy nanoclusters (AuAg/PtAg) have been synthesized. They enhance the electrochemiluminescence of luminal for about 20 times in pH 8.5 solution. It extended the practicable pH range of ECL and can be employed for detecting the oxygen matters and other related trace compounds.
研究领域:
分析化学
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环境科学
·
生物医学
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产品关联
DOI
高影响因子
LUMINESCENCE
·
2016
·Vol. 32
·期 3
·277-284
·
IF 1.452
Jianqiu Chen,Juan Shu,Jiao Chen,Zhiran Cao,An Xiao,Zhengyu Yan
S,N co-doped carbon quantum dots (N,S-CQDs) with super high quantum yield (79%) were prepared by the hydrothermal method and characterized by transmission electron microscopy, photoluminescence, UV–Vis spectroscopy and Fourier transformed infrared spectroscopy. N,S-CQDs can enhance the chemiluminescence intensity of a luminol–H 2 O 2 system. The possible mechanism of the luminol–H 2 O 2 –(N,S-CQDs) was illustrated by using chemiluminescence, photoluminescence and ultraviolet analysis. Ranitidine can quench the chemiluminescence intensity of a luminol–H 2 O 2 –N,S-CQDs system. So, a novel flow-injection chemiluminescence method was designed to determine ranitidine within a linear range of 0.5–50 μg ml −1 and a detection limit of 0.12 μg ml −1 . The method shows promising application prospects.
研究领域:
分析化学
·
药物分析
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产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）