INTERNATIONAL IMMUNOPHARMACOLOGY
·
2025
·
IF 4.7
Zhiqiang Wang,Man Li,Shuming Guo,Chen Deng,Beiyang Wang,Fang Gao,Junqiao Lv,Lin Sun
Ischemia and hypoxia near the injury site following spinal cord injury (SCI) lead to early lactate accumulation. Reactive astrocytes polarize and migrate to the lesion site, sealing it off and restricting immune cell infiltration. However, the specific mechanisms through which lactate influences astrocyte polarization remain poorly understood. In this study, we found that lactate can promote astrocyte proliferation, migration and differentiation into A2 after oxygen-glucose deprivation/reoxygenation (OGD/R). Inhibiting signal transducer and activator of transcription 3 (STAT3) nuclear transport reversed lactate-driven astrocyte polarization both in vivo and in vitro. Moreover, lactylome analysis identified nonhistone protein Ran as a target of lactylation at lysine 123 (K123). Ran silencing or mutation at K123 reversed the effects of lactate on STAT3 nuclear transport and astrocyte polarization. We also found that Ran lactylation is regulated by SIRT1. Taken together, our results show that lactate promotes astrocyte polarization via Ran lactylation-dependent regulation of STAT3 nuclear transport.
研究领域:
神经科学
·
细胞生物学
·
分子生物学
·
代谢学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POULTRY SCIENCE
·
2020
·
IF 2.659
Mingfa Sun,Hongchao Jiao,Xiaojuan Wang,Victoria A. Uyanga,Jingpeng Zhao,Hai Lin
Crystalline amino acids ( AAs ) exhibit high nutritional values when supplemented AA-deficient diets. However, the AAs in crystalline form in the diet are absorbed quickly than protein-bound AAs, which may take an effect on AA utilization efficiency. In this study, 2 experiments were conducted to investigate the effect of encapsulated lysine-HCl ( Ly s) and DL-methionine ( DL-Met ) on the growth performance of broiler chickens. In experiment 1, a total of 432 one-day-old male Arbor Acres broilers were subjected to 3 dietary treatments (27 pens; 16 birds per pen) for 42 d. The control group was basal diets supplemented with crystalline Lys and DL-Met, and treatment groups had basal diets supplemented with encapsulated Lys and DL-Met at the levels of 80% and 60% of control diets ( 80CLM , 60CLM ), respectively. The growth performance, intestinal development, and transcription of AA transporters were determined. In experiment 2, 24 broiler chickens were subjected to the same treatments as in experiment 1. The plasma concentrations of free AAs were measured 0, 2, 4, and 6 h after feeding. The results showed that 80CLM treatment had no significant influence on production performance, carcass characteristics, and plasma free AAs content during the experiment compared with the control group ( P > 0.05). In addition, the 80CLM group moderately enhanced gut morphology development and increased AAs' absorption capacity. However, broilers fed the 60CLM diet had lower production performance and breast muscle weight than the control group ( P < 0.05), but increased villi height and B 0 AT mRNA expression level ( P < 0.05). At h 4 after feeding, the 60CLM broilers exhibited higher concentration of Ala, Cys, and total dispensable AAs than the control group ( P < 0.05). In conclusion, the result suggests that the supplemental levels of crystalline Lys and DL-Met can be effectively saved approximately for 20% by using the encapsulated form in broilers, with improvements to AAs utilization efficiency, while posing no detrimental effects on production performance. Encapsulated Lys and DL-Met would have greater potential for application when replacing crystalline AAs in broiler chickens.
研究领域:
动物营养学
·
家禽科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOTECHNOLOGY LETTERS
·
2016
·Vol. 38
·期 7
·1121-1129
·
IF 1.639
Zhou Jian Qin,He Ting,Wang Jian Wen
Objectives To establish a method for microbial trans glutaminase (mTG)-mediated PEGylation of proteins at the level of lysine (Lys) residues. Results Carboxybenzyl-glutaminyl–glycinyl-methoxypolyethylene glycol (CBZ-QG-mPEG) was prepared by introducing carboxybenzyl-glutaminyl-glycine (CBZ-QG) to mPEG amine. The analysis by Fourier transform infrared spectroscopy and SDS-PAGE showed that CBZ-QG-mPEG was successfully synthesized and can be recognized by mTG as an acyl donor to modify therapeutic protein, cytochrome c (cyt c ). Finally, under an optimized condition (cyt c 0.5 mg/ml, CBZ-QG-mPEG 11.25 mg/ml, mTG 0.5 mg/ml, 37 °C, 2 h), the PEGylation yield reached 76.5 %. Conclusions This is the first study regarding the PEGylation of protein at the level of Lys residues catalyzed by mTG. The novel method could be employed to immobilize active proteins and modify therapeutic proteins.
研究领域:
生物化学
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蛋白质工程
·
药学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Inorganic Chemistry Frontiers
·
2022
·Vol. 9
·期 15
·3679-3691
·
IF 7.779
Lei Fan,Lianming Zhao,Yuchao Lv,Tinghu Wang,Yupeng Tian,Jianye Fu,Xinmei Liu
The synthesis of highly-dispersed non-noble metal based catalysts and their application in hydrogenation/dehydrogenation still remain challenging. Therefore, it is of great importance to protect metals from aggregation in both calcination and reduction processes. In this research, density functional theory (DFT) calculations were used to elucidate the stabilization effect of ionic liquid [Bmim][BF4] on Ni in the reduction process, showing that [Bmim][BF4] could efficiently inhibit Ni aggregation on the support. By further analysis combined with theoretical calculations, we found that the degree of metal dispersion before reduction directly determines the effect of [Bmim][BF4]. Guided by the DFT results, the method of co-assisted preparation of Ni/SAPO-11 using [Bmim][BF4] and lysine was adopted. Lysine forms a coordination compound with nickel oxides. The steric hindrance generated by the Ni–lysine complex can effectively inhibit the aggregation of nickel oxides during the calcination process. The DFT calculations revealed that during the reduction process, [BF4−] plays a dominant role in stabilization and electronic modulation by directly interacting with Ni on the support. The combination effects of [Bmim][BF4] and lysine effectively inhibit metal aggregation, leading to ultra-small nickel particles with a mean size of 4.4 nm over the obtained catalyst. Furthermore, the presence of [Bmim][BF4] also tunes the electronic state of Ni species. Using hexane isomerization as a probe reaction, the result indicates the excellent catalytic activity of the obtained catalyst (≈93.7% isomer selectivity at 63.5% conversion), much superior to that of Ni/SAPO-11 obtained from the traditional impregnation route (≈75.8% isomer selectivity at 64.6% conversion). This work provides a new method for the preparation of highly effective non-noble metal supported catalysts.
研究领域:
催化化学
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材料科学
·
理论计算
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF POWER SOURCES
·
2014
·
IF 5.211
Rongfang Wang,Tianbao Zhou,Hui Wang,Hanqing Feng,Shan Ji
N-doped carbon nanotubes are highly favored for use as electrocatalysts for oxygen reduction reaction (ORR) because of their relatively low cost and superior catalytic activity . Here, Lysine-derived porous carbon nanotubes (LPCNs) with large inner cavities of ca. 300–400 nm are reported as electrocatalyst for ORR. LPCNs exhibit 28.3 mV of more positive half-wave-potential than that of commercial Pt/C. In addition, LPCNs is much more stable and tolerant to fuel crossover effect than Pt/C. The above superiorities make it a promising candidate for substituting noble metal catalysts as a cathode catalyst for ORR.
研究领域:
材料科学
·
电化学
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Science
·
2016
·Vol. 4
·期 3
·522-532
·
IF 3.614
Shuai Liu,Jixiang Yang,Hongqi Ren,Jonathan O'Keeffe-Ahern,Dezhong Zhou,Hao Zhou,Jiatong Chen,Tianying Guo
Natural polycations, such as poly(L-lysine) (PLL) and chitosan (CS), have inherent superiority as non-viral vectors due to their unparalleled biocompatibility and biodegradability. However, the application was constrained by poor transfection efficiency and safety concerns. Since previous modification strategies greatly weakened the inherent advantages of natural polycations, developing a strategy for functional group introduction with broad applicability to enhance the transfection efficiency of natural polycations without compromising their cationic properties is imperative. Herein, two uncharged functional diblock oligomers P(DMAEL-b-NIPAM) and P(DMAEL-b-Vlm) were prepared from a lactose derivative, N-iso-propyl acrylamide (NIPAM) as well as 1-vinylimidazole (Vlm) and further functionalized with four small ligands folate, glutathione, cysteine and arginine, respectively, aiming to enhance the interactions of complexes with cells, which were quantified utilizing a quartz crystal microbalance (QCM) biosensor, circumventing the tedious material screening process of cell transfection. Upon incorporation with PLL and DNA, the multifunctional oligomers endow the formulated ternary complexes with great properties suitable for transfection, such as anti-aggregation in serum, destabilized endosome membrane, numerous functional sites for promoted endocytosis and therefore robust transfection activity. Furthermore, different from the conventional strategy of decreasing cytotoxicity by reducing the charge density, the multifunctional oligomer incorporation strategy maintains the highly positive charge density, which is essential for efficient cellular uptake. This system develops a new platform to modify natural polycations towards clinical gene therapy.
研究领域:
生物材料学
·
基因治疗
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2017
·
IF 3.387
Wenting Li,Qi Liu,Jingyuan Liu,Hongsen Zhang,Rumin Li,Zhanshuang Li,Xiaoyan Jing,Jun Wang
Polyacrylonitrile fibers (PANF) covalently modified with lysine (PAN-Lys) was facilely synthesized and carefully characterized. The critical factors affecting U(VI) adsorption from aqueous solution were exploited, such as initial pH, contact time, concentration and temperature. The adsorption process is strongly dependent on solution pH. With excellent adsorption capacity and high affinity toward U(VI), the process for U(VI) is extremely rapid and the equilibrium can be reached within 20 min. The thermodynamics and kinetics were strictly evaluated. In addition, the hypothetical adsorption mechanisms were proposed. Moreover, the adsorption behavior at low concentrations (3–30 μg L −1 ) in simulated seawater was also investigated. Therefore, PAN-Lys can be potentially utilized for the efficient removal of U(VI) from seawater.
研究领域:
环境科学
·
材料科学
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2021
·
IF 5.279
Zhiqiang Lu,Xiaomeng Xu,Dongmei Li,Na Sun,Songyi Lin
Memory impairment is becoming a potential health issue with the delicacy of diet and social stress. Sea cucumber peptides (SCP) prevent memory impairment, as previously reported. In this study, further research was performed using hippocampal lysine-acetylome to explore molecular regulation mechanisms. C57BL/6 mice were treated with scopolamine via intraperitoneal injection to simulate memory impairment. To determine the influence of SCP on the total acetylated-protein level of the hippocampus, acetylated-proteomics was performed. SCP increased the acetylation level of histone (H3 and H4). Meanwhile, for non-histones, the differentially acetylated proteins were involved in multiple memory-related pathways, as shown by KEGG enrichment analysis. Additionally, long-term potentiation was confirmed by western blotting. Finally, a combined analysis of proteome and lysine acetylome revealed that SCP contributed to synaptic vesicle cycle regulation and dopamine metabolism. Consequently, our findings revealed that SCP was potentially neuroprotective by regulating post-transcriptional hippocampal protein acetylation.
研究领域:
神经科学
·
蛋白质组学
·
分子生物学
·
药理学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
POLYMERS FOR ADVANCED TECHNOLOGIES
·
2021
·Vol. 33
·期 2
·534-545
·
IF 3.665
Chen Cheng,Yanling Lu,Junfeng Cai,Jun Yan,Shaojie Li,Shiguo Du
To improve the flame retardance and compatibility of ammonium polyphosphate (APP) via an environmentally friendly method, l -lysine (Lys), a bio-based amino acid, was selected as the surface-modifier for APP in this work. Lys-modified APP (L-APP) was prepared through the cation exchange reaction, and its morphology and chemical composition were characterized comprehensively. The characterization results demonstrated the fabrication of Lys shell structure on the surface of APP. Additionally, the thermogravimetric analysis illustrated that the char-forming ability of APP was significantly improved by the Lys shell structure. The flame retardance of APP and L-APP on epoxy resin (EP) was evaluated by the limiting oxygen index (LOI), vertical burning (UL-94), and cone calorimeter tests (CCT). The results showed that EP blending with 15 wt% L-APP (EP/L-APP15) passed V-0 rating, and got an LOI value of 31.7%. In the CCT, compared to the pristine EP, the peak heat release rate and total smoke production of EP/L-APP15 were decreased by 77.6% and 47.1%, respectively. It was confirmed that the good flame retardance and smoke suppression performance of L-APP can be attributed to the phosphorus-containing intumescent char residuals formed during combustion process. The tensile strain tests were conducted to assess the mechanical properties of EP composites. The results demonstrated that compared to pristine APP, L-APP possessed weakened impact on the tensile strain property of EP, which was beneficial to the practical application.
研究领域:
材料科学
·
化学工程
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2020
·Vol. 95
·期 5
·1542-1549
·
IF 2.75
Yuxuan Liu,Yunxin Zheng,Hao Wu,Wei Zhang,Tianyu Ren,Shengping You,Wei Qi,Rongxin Su,Zhimin He
BACKGROUND Cadaverine is a natural polyamine that can replace fossil-derived hexamethylene diamine to produce bio-based polyamide. Despite huge developments in cadaverine bioproduction, an econo"mically attractive purification process is a highly important part of the production of high-purity cadaverine. RESULTS This study utilized l -lysine hydrochloride as a substrate for the production of high-purity cadaverine via a litre-scale integrated process incorporating fermentation, bioproduction, deprotonation, extraction and rectification. First, 8.24 U mg –1 lysine decarboxylase activity and 205 g L –1 cadaverine with a yield of 91.61% were achieved through the fermentation of low-cost industrial culture by engineered Escherichia coli and the bioproduction of cadaverine by lysine decarboxylase. Secondly, four integrated purification methods – deprotonation-evaporation, pH adjustment-deprotonation-evaporation, deprotonation-extraction-evaporation and deprotonation-extraction-rectification – were explored, and the conditions of each unit operation in the chosen method (deprotonation-extraction-rectification) were optimized. Then the optimized method was applied to a 2-L purification system, resulting in 99% purity of cadaverine with a yield of 87.47%. Furthermore, the n -butanol recovery ratio reached 91.25%, effectively avoiding the waste of resources and environmental pollution, and thus reducing purification costs. CONCLUSION The proposed experimental approach proves that this integrated process leads to the production of high-purity cadaverine. In the 2-L-scale integrated process, 358.64 g cadaverine of 99% purity was produced at a cost of US$3.99 (US$1.11 per 100 g), which is far below its market price (US$784.71 per 100 g). This integrated process provides a potential way for the industrial-scale production of bio-based cadaverine. © 2020 Society of Chemical Industry
研究领域:
生物工程
·
化学工业
✓ 已验证
产品关联
DOI
高影响因子
Applied Materials Today
·
2020
·
IF 8.352
Peili Li,Fengxuan Han,Weiwei Cao,Gaoke Zhang,Jiaying Li,Jinwei Zhou,Xuedong Gong,Gareth Turnbull,Wenmiao Shu,Lunguo Xia,Bing Fang,Xiaodong Xing,Bin Li
Effectively targeting and killing microbial pathogens is a major challenge in the treatment of infectious diseases. Inspired by the high efficacy of natural antimicrobial peptides (AMPs), in this study we prepared two types of carbon quantum dots (CQDs) by pyrolysis of lysine and arginine, the two major functional moieties of natural AMPs. Both lysine-derived CQDs (Lys-CQDs) and arginine-derived CQDs (Arg-CQDs) exhibited distinctive antibacterial activity against both Gram-negative and Gram-positive bacteria without inducing drug resistance. They also effectively inhibited the formation of bacterial biofilms. Such excellent antibacterial properties might be the result of positive charges derived from the amino acid residues on CQD surface and the generation of rich intracellular reactive oxygen species (ROS). Surprisingly, instead of being cytotoxic, CQDs dramatically promoted the growth of typical mammalian cells. Moreover, no hemolysis of red blood cells (RBCs) happened when CQD concentration was less than 4,000 μg/mL. In vivo , administration of CQDs to infected wounds effectively inhibited bacterium growth and accelerated wound healing. Together, findings from this study indicate that Lys-CQDs and Arg-CQDs can simultaneously kill bacteria and facilitate cell growth, and may therefore be promising candidates for treating bacterial infections and promoting tissue repair.
研究领域:
材料科学
·
生物医学工程
·
微生物学
✓ 已验证
DOI
高影响因子
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
·
2019
·Vol. 188
·期 3
·769-786
·
IF 2.14
Li Chan,Zhang Wen,Yang Ning,Zhang Qing Song
As one of the typical phyllosilicate clays, hectorite (Hec) has some excellent characteristics and has been greatly applied in adsorption field for the removal of dye, endotoxin, etc. In this study, organic Hec nanocomposites modified with l -Lysine (Lys/Hec NCs) were prepared via solution intercalation method for BR removal. The effects of ionic strength, pH values, initial concentration of BR, and BSA concentration on the adsorption capacity for BR of Lys/Hec NCs were investigated. Results indicated that the adsorption capacity for BR of nanocomposites could reach 40 mg/g when the initial bilirubin concentration was 200 mg/L. However, the adsorption amount of Lys/Hec NCs decreased with increasing the concentration of BSA, but Lys/Hec NCs could still maintain a higher adsorption rate. The adsorption kinetics and adsorption isotherms indicated that the adsorption process of Lys/Hec NCs agreed well with the pseudo-second-order model and the Langmuir isotherm, respectively. Moreover, Lys/Hec NCs also exhibited excellent cytocompatibility. These obtained results demonstrate that Lys/Hec NCs prepared in this study had great potential to be used in hemoperfusion.
研究领域:
生物医学工程
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）