Food Chemistry-X
·
2024
·
IF 6.5
Qian Zhang,Yu-Qiao Wang,Lin Li,Hao-Lin Song,Hai-Tao Wu,Bei-Wei Zhu
Salidroside (Sal), the main bioactive substance in Rhodiola rosea , is a promising functional food component with a wide range of pharmacological effects, but its biological activity is challenging to sustain due to its short half-life, low oral bioavailability, and susceptibility to environmental factors. The aim of this study was to investigate the effect of sodium alginate (SA) concentration on the construction of W/O/W emulsion in the protection of Sal. With the escalation of SA concentrations, the range of droplet size distribution was smaller and the droplets were more uniform. When the concentration of SA was 2 %, the average droplet size reached 9.1 ± 0.1 μm, and the encapsulation efficiency of Sal was 77.8 ± 1.8 %. Moreover, the double emulsion with 2 % SA was the most stable for 28 days at 4 °C since the oil droplets were embedded in the network structure of SA.
研究领域:
药学
·
食品科学
·
材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACTA PHARMACOLOGICA SINICA
·
2024
·
IF 6.9
Zhang Chen-chun,Li Yuan,Jiang Chang-you,Le Qiu-min,Liu Xing,Ma Lan,Wang Fei-fei
The O-linked-β-N-acetylglucosamine (O-GlcNAc) glycosylation (O-GlcNAcylation) is a critical post-translational modification that couples the external stimuli to intracellular signal transduction networks. However, the critical protein targets of O-GlcNAcylation in oxidative stress-induced apoptosis remain to be elucidated. Here, we show that treatment with H 2 O 2 inhibited O-GlcNAcylation, impaired cell viability, increased the cleaved caspase 3 and accelerated apoptosis of neuroblastoma N2a cells. The O-GlcNAc transferase (OGT) inhibitor OSMI-1 or the O-GlcNAcase (OGA) inhibitor Thiamet-G enhanced or inhibited H 2 O 2 -induced apoptosis, respectively. The total and phosphorylated protein levels, as well as the promoter activities of signal transducer and activator of transcription factor 3 (STAT3) and Forkhead box protein O 1 (FOXO1) were suppressed by OSMI-1. In contrast, overexpressing OGT or treating with Thiamet-G increased the total protein levels of STAT3 and FOXO1. Overexpression of STAT3 or FOXO1 abolished OSMI-1-induced apoptosis. Whereas the anti-apoptotic effect of OGT and Thiamet-G in H 2 O 2 -treated cells was abolished by either downregulating the expression or activity of endogenous STAT3 or FOXO1. These results suggest that STAT3 or FOXO1 are the potential targets of O-GlcNAcylation involved in the H 2 O 2 -induced apoptosis of N2a cells.
研究领域:
细胞生物学
·
分子生物学
·
神经科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2024
·
IF 13.3
Wenqian Cao,Yumeng Qi,Ying Zhang,Ruixue Guo,Ruijuan Qu,Zunyao Wang
Photolysis is the main pathway for the degradation of persistent organic pollutants (POPs). Most investigations, however, have been limited to the ultraviolet or visible region, and the reaction mechanism of infrared (IR) light is still unclear. In this study, the photodegradation of o,p' -dichlorodiphenyltrichloroethanes ( o,p' -DDT) on the silica (SiO 2 )/air interface under different irradiation light sources was systematically investigated. Interestingly, for the first time, effective degradation of o,p' -DDT under IR light irradiation was observed, which was ascribed to the anti-Stokes Raman scattering process of SiO 2 . Moreover, desert sands could also serve as a vector for the photodegradation of o,p' -DDT. The lower removal efficiency of desert sands compared to pure SiO 2 was a combined effect of differences in Fe 3+ component and SiO 2 content. It is worth noting that direct photodegradation plays a dominant role in the photodegradation of o,p' -DDT on SiO 2 . Besides, indirect photodegradation resulted mainly from •OH (25.0%), O 2 • − (6.9%), and 1 O 2 (5.6%) generated in the reaction system. In addition to hydroxylation and dechlorination products, the formation of four novel silicon-based products was observed, which resulted from the direct reaction of silyl radicals with o,p' -DDT. This study revealed that the specific Raman scattering effect of SiO 2 played a vital role in the self-purification process of POPs upon SiO 2 -based environmental matrices. The light-mediated decontamination system upon SiO 2 constructed in this study could provide critical information for addressing POPs contamination on sands.
研究领域:
环境科学
·
化学
✓ 已验证
产品关联
DOI
高影响因子
Food Bioscience
·
2023
·
IF 5.2
Mei Bai,Jinming Dai,Wei Hu,Changzhu Li,Haiying Cui,Lin Lin
This study investigated the use of Litsea cubeba essential oil (LC-EO) as an anti-virulence agent targeting listeriolysin O (LLO) of Listeria monocytogenes ( L. monocytogenes ). The hemolysis assay showed a significant decrease in the hemolytic activity of LLO by 90.7% and 70.2% after exposure to 500 μg mL −1 LC-EO or 700 μg mL −1 citral (the main component of LC-EO), respectively. This suggested that citral plays a major role in the anti-virulence function against LLO. The experiments including UV–visible absorption spectroscopy , fluorescence quenching and three-dimensional fluorescence spectroscopy demonstrated that exposure to citral leads to significant changes in the conformation of LLO, potentially resulting in the unfolding of its structure and the formation of a ground-state complex. Synchronous fluorescence spectra analysis indicated that the binding site of citral could be the undecapeptide region of LLO. Additionally, the results of molecular docking and the acid-base effect experiment of different aldehydes and phenols revealed that the cysteine residue of LLO and the α, β-unsaturated aldehyde of citral are likely to form a covalent bond through Michael addition . This interaction is believed to be the primary reason for the loss of hemolytic activity. As a result, this work provides a new approach for the development of LLO-targeting anti-virulence agents and holds promise for expanding the application of LC-EO in the field of food microbiological safety.
研究领域:
食品科学
·
微生物学
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产品关联
DOI
高影响因子
ACS Applied Materials & Interfaces
·
2020
·
IF 8.758
Yuehan Cao,Ruiyang Zhang,Tianli Zhou,Shengming Jin,Jindi Huang,Liqun Ye,Zeai Huang,Fang Wang,Ying Zhou
Limited by the chemical inertness of CO2 and the high dissociation energy of the C═O bond, photocatalytic CO2 conversion is highly challenging. Herein, we prepare ultrathin oxygen-modified h-BN (O/BN) nanosheets containing B–O bonds. On the O/BN surface, CO2 can be chemically captured and is bonded with the B–O bond, leading to the formation of an O–B–O bond. This new chemical bond acting as an electron-delivery channel strengthens the interaction between CO2 and the surface. Thus, the reactants can continuously obtain electrons from the surface through this channel. Therefore, the majority of gaseous CO2 directly converts into carbon active species that are detected by in situ DRIFTS over O/BN. Moreover, the activated energies of CO2 conversion are significantly reduced with the introduction of the B–O bond evidenced by DFT calculations. As a result, O/BN nanosheets present an enhanced photocatalytic CO2 conversion performance with the H2 and CO generation rates of 3.3 and 12.5 μmol g–1 h–1, respectively. This work could help in realizing the effects of nonmetal chemical bonds in the CO2 photoreduction reaction for designing efficient photocatalysts.
研究领域:
材料科学
·
环境科学
✓ 已验证
DOI
PubMed
高影响因子
ACS Catalysis
·
2025
·
IF 13.1
Yifan Li,Jiaxing Li,Lin Chen,Yunpeng Long,Xing Yuan,Junhua Li,Yue Peng
The low selectivity for N2 in the oxidation of NH3 over commercial Pt/Al2O3 catalysts is primarily due to the overoxidation of NH3 facilitated by Pt sites, leading to the formation of unwanted byproducts such as N2O and NO. In this study, we present a novel strategy to enhance N2 selectivity while maintaining NH3 conversion by constructing Pt–O–Cu dual sites. These dual sites exhibit synergistic acid-redox characteristics through surface electron transfer mediated by bridged lattice oxygen. Additionally, the ability of surface-adsorbed oxygen to exchange with lattice oxygen is significantly improved. The electron-deficient Cu sites enhance NH3 adsorption by providing empty 3d orbitals, while the electron-rich Pt sites promote NH3 dehydrogenation. Subsequently, the formation of –NH or –N intermediates at the Pt sites can react with adsorbed NH3 on the Cu sites to produce N2, predominantly following the integrated selective catalytic reduction mechanism. The optimized dual-site catalyst achieves over 95% NH3 conversion and N2 selectivity at 180 °C.
研究领域:
催化化学
·
环境科学
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2017
·
IF 2.986
Hanqin Liang,Kaihui Zuo,Yongfeng Xia,Dongxu Yao,Jinwei Yin,Yuping Zeng
Lu-Al-Si-O-N tapes with different thickness were used to join gas pressure sintered Si 3 N 4 ceramics. The microstructure of the joints and the influences of the joint thickness and joining temperature on the bonding strength of the as-joined Si 3 N 4 ceramics have been investigated. The highest bonding strength about ~ 300 MPa of the joined specimens was achieved by using 450 µm interlayer at 1450 °C. The existence of Si 3 N 4 nanowires was beneficial for the improvement of the bonding strength by interweaving the oxynitride glass matrix in the joint region.
研究领域:
材料科学
·
陶瓷工程
✓ 已验证
产品关联
DOI
高影响因子
TALANTA
·
2020
·
IF 5.339
Xue Han,Zhen Meng,Lian Xia,Fengli Qu,Rong-Mei Kong
This study demonstrates a novel and convenient ratiometric fluorescent method for the detection of alkaline phosphatase (ALP) activity. Amino-functionalized mesoporous silica nanoparticle-gold nanoclusters (MSN-AuNCs) nanocomposites were integrated with o-phenylenediamine (OPD) to form a ratiometric fluorescence nanoplatform. The presence of ALP induced the generation of quinoxaline (QX) derivative which called 3-(dihydroxyethyl)furo[3,4-b]quinoxaline-1-one (DFQ) with strong fluorescence emission at 450 nm, while the orange-red fluorescence of MSN-AuNCs at 580 nm was slightly quenched. Meanwhile, an obvious fluorescence color change from orange-red to purple and finally to blue can be observed by naked eyes with the increasing of ALP concentration. Therefore, employing the fluorescence emission of DFQ at 450 nm as the reporter signal and the fluorescence emission of MSN-AuNCs at 580 nm as a reference signal, a sensitive ratiometric detection method for ALP was developed. Quantitative detection of ALP activity in the linear range from 0.2 to 80 U/L with a detection limit of 0.1 U/L can be realized in this way, which endows the assay with high sensitivity enough for practical detection of ALP in human serum samples.
研究领域:
生物化学
·
纳米医学
·
分析化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
APPLIED SURFACE SCIENCE
·
2021
·
IF 6.707
Weili Teng,Qinqin Zhou,Xuekai Wang,Haibing Che,Yucheng Du,Peng Hu,Hongyi Li,Jinshu Wang
Heteroatom-doped hierarchically porous carbon is attractive electrode material for supercapacitors. However, it still remains a great challenge to develop a high-performance supercapacitor electrode with efficient preparation strategy. Herein, we report a N,O-codoped hierarchically porous carbon (NOHPC) prepared through one-step carbonization/in-situ doping/activation process by using natural diatomite as template and polydopamine as precursor. The as-prepared NOHPC possesses synergistic advantages of abundant redox-active N,O dopants, large effective specific surface area and hierarchically porous (micropores, mesopores and macropores) structure. Benefiting from such advantages, the NOHPC electrode exhibits an extremely high specific capacitance of 436.0 F g −1 at 0.625 A g −1 in H 2 SO 4 electrolyte. The as-assembled supercapacitor in H 2 SO 4 electrolyte also yields high specific capacitance (328.3 F g −1 at 0.625 A g −1 ), excellent rate performance (74.8% capacitance retention, 0.625–25 A g −1 ) and superb cycling stability (93.9%, 10,000 cycles). Besides, the corresponding device in Na 2 SO 4 electrolyte can present an enhanced energy density of 23.4 Wh kg −1 at the power density of 358.8 W kg −1 (1 A g −1 ) and still maintain 15.0 Wh kg −1 at the power density of 8357.8 W kg −1 (25 A g −1 ). The outstanding electrochemical performance and the efficient preparation process of NOHPC make it a promising widely applied electrode material for supercapacitor.
研究领域:
材料科学
·
能源存储
·
纳米技术
✓ 已验证
产品关联
DOI
高影响因子
FOOD CHEMISTRY
·
2017
·
IF 4.529
Lei Zhou,Zhiqiang Xiong,Wei Liu,Liqiang Zou
Gentisic acid and cyanidin-3-O-glucoside are important bioactive polyphenols which are widely distributed in many fruits and cereals. In this work, kinetic study, spectral analysis and computational simulation were used to compare the inhibitory effects and inhibition mechanisms of gentisic acid and cyanidin-3-O-glucoside on mushroom polyphenoloxidase (PPO). The inhibitory effect of cyanidin-3-O-glucoside on PPO was much stronger than that of gentisic acid. Gentisic acid inhibited PPO in a reversible mixed-type manner while cyanidin-3-O-glucoside was an irreversible inhibitor. Gentisic acid and cyanidin-3-O-glucoside made the thermal inactivation of PPO easier, and induced apparent conformational changes of PPO. Compared with gentisic acid, cyanidin-3-O-glucoside had stronger effects on the thermal inactivation and conformation of PPO. Molecular docking results revealed gentisic acid bound to the active site of PPO by hydrogen bonding, π–π stacking and van der Waals forces. However, cyanidin-3-O-glucoside might irreversibly interact with the Met or Cys in PPO by covalent bonds.
研究领域:
生物化学
·
食品科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of the American Chemical Society
·
2024
·
IF 14.4
Liu Lin,Yunming Xu,Yiting Han,Ruikun Xu,Tongyue Wang,Zemin Sun,Zhenhua Yan
A deep understanding of the mechanism for the spin-magnetic effect on O–H cleavage is crucial for the development of new catalysts for water oxidation. Herein, we designed and synthesized the crystalline Fe-DABDT and Co-DABDT (DABDT = 2,5-diaminobenzene-1,4-dithiol) and optimized an effective magnetic moment to explore the role of the spin-magnetic effect in the regulation of water oxidation activity. It can be found that the OER activity of the catalyst is positively correlated with its effective magnetic moment. Under the external magnetic field, Fe-DABDT with more spin single electrons has a stronger spin-magnetic response to water oxidation than Fe/Co-DABDT and Co-DABDT. The increase in OER current of Fe-DABDT is nearly 2 times higher than that of Co-DABDT. Experimental and density functional theory studies show that magnetized Fe sites could realize nucleophilic reaction, accelerate the polarization of electron spin states, and promote the polar decomposition of O–H and the formation of the O–O bond. This study provides mechanistic insight into the spin-magnetic effect of oxygen evolution reaction and further understanding of the spin origin of catalytic activity, which is expected to improve the energy efficiency of hydrogen production.
研究领域:
化学
·
材料科学
·
催化反应
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ChemSusChem
·
2016
·Vol. 9
·期 22
·3220-3229
·
IF 7.116
Haiwei Guo,Bo Zhang,Changzhi Li,Chang Peng,Tao Dai,Haibo Xie,Aiqin Wang,Tao Zhang
Graphical Breaking up : Tungsten carbide shows remarkable activity in selective C−O bond cleavage of typical β-O-4 model compounds and deconstruction of lignin feedstocks. High yields of C−O cleavage products from model compounds (up to 96.8 %) and from lignin feedstock (up to 70.7 %) are obtained without destroying the aromatic rings. A remarkably effective method for the chemoselective cleavage of the C−O bonds of typical β-O-4 model compounds and the deconstruction of lignin feedstock was developed by using tungsten carbide as the catalyst. High yields of C−O cleavage products (up to 96.8 %) from model compounds and liquid oils (up to 70.7 %) from lignin feedstock were obtained under low hydrogen pressure (0.69 MPa) in methanol. The conversion efficiency was determined to a large extent by solvent effects and was also affected by both the electronic and steric effects of the lignin model compounds. In situ W 2 C/activated carbon (AC)-catalyzed hydrogen transfer from methanol to the substrate was proposed to be responsible for the high performance in methanol solvent. The conversion of 2-(2-methoxyphenoxy)-1-phenylethanol showed that the catalyst could be reused five times without a significant loss in activity for C−O bond cleavage, whereas the selectivity to value-added styrene increased markedly owing to partial oxidation of the W 2 C phase according to X-ray diffraction, Raman spectroscopy, and transmission electron microscopy characterization. 2 D-HSQC-NMR spectroscopy analysis showed that W 2 C/AC exhibited high activity not only for β-O-4 cleavage but also for the deconstruction of more resistant α-O-4 and β-β linkages, so that a high yield of liquid oil was obtained from lignin. Corn stalk lignin was more liable to be depolymerized than birch lignin owing to its loosened structure (scanning electron microscopy results), larger surface area (BET results), and lower molecular weight (gel-permeation chromatography results), whereas its liquid oil composition was more complicated than that of birch wood lignin in that the former lignin contained more p -hydroxyphenyl units and the former contained noncanonical units.
研究领域:
化学工程
·
材料科学
·
生物质能
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）