INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2023
·
IF 8.2
Jingna Liu,Bingqing Chen,Qianyi Hu,Qiong Zhang,Bingqing Huang,Peng Fei
To augment the functional attributes of pectin and expand its prospective utilization in food preservation, this research explored the enzymatic grafting of resorcinol and 4-hexylresorcinol onto pectin. Structural analysis verified the successful grafting of both resorcinol and 4-hexylresorcinol to pectin via esterification , with the 1-OH of resorcinol and 4-hexylresorcinol and the carboxyl group of pectin functioning as grafting sites. The grafting ratios of resorcinol-modified pectin (Re-Pe) and 4-hexylresorcinol-modified pectin (He-Pe) were 17.84 % and 10.98 %, respectively. This grafting modification notably enhanced the antioxidative and antibacterial properties of pectin. Specifically, DPPH clearance and the inhibition ratio in the β-carotene bleaching assay increased from 11.38 % and 20.13 % (native pectin, Na-Pe) to 41.15 % and 36.67 % (Re-Pe), and 74.72 % and 53.40 % (He-Pe). Moreover, the inhibition zone diameter against Escherichia coli and Staphylococcus aureus rose from 10.12 and 10.08 mm (Na-Pe) to 12.36 and 11.52 mm (Re-Pe), and 16.78 and 14.87 mm (He-Pe). Additionally, the application of native and modified pectin coatings effectively impeded pork spoilage, with the modified pectins demonstrating a more potent effect. Among the two modified pectins, He-Pe exhibited the most significant enhancement in pork shelf life.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2022
·
IF 8.025
Ya Li,Xi-xiang Shuai,Ming Zhang,Fei-yue Ma,Jun Chen,Jian Qiao,Rong-hao Chen,Li-qing Du
As a common macromolecular carbohydrate, pectin has a strong affinity for Pb 2+ . An ethylenediamine modified pectin (EP 48 ) with 48 % of amidation was prepared and exhibited great removal efficiency towards Pb 2+ in our previous study. However, the EP 48 has drawbacks in adsorption including low mechanical strength and difficulty in separation. In this study, EP 48 was compounded with sodium alginate (Alg) and Fe 3 O 4 to synthesize EP 48 /Alg/Fe 3 O 4 microsphere. The physicochemical properties and Pb 2+ adsorption characteristics of microsphere were analyzed. It was found that the microsphere exhibited good thermal stability, mechanical strength, porous structure, as well as acid tolerance. The pseudo-second-order model well described the kinetics of adsorption process, indicating the chemical adsorption is dominant. The Langmuir model fitted the experimental data well, and the maximum adsorption capacity reached 175.19 mg/g. Adsorption-desorption experiments showed that the removal rate of the microsphere maintained over 98.9 % after 10 cycles. The X-ray photoelectron spectroscopy (XPS) analyses revealed that the potential adsorption mechanism included ion-exchange and chelation. The above results suggested its potential use for the removal of Pb 2+ from wastewater.
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Analytical Methods
·
2022
·Vol. 14
·期 23
·2266-2276
·
IF 3.532
Huan Zhang,Qi Guan,Pei Zeng,Guoqiang Wu,Yanping Hong,Wuying Yang,Chunrong Wang
In the present study, a novel simple and sensitive method was developed for the determination of pectin based on the decarboxylation derivatization reaction and surface-enhanced Raman scattering (SERS) without complicated separation steps. The derivatization reaction can be controlled by the experimental parameters such as reaction time, temperature and the amount of hydrochloric acid. Additionally, the method was used to accurately and reliably detect pectin added in honey or apple, which can be detected at levels as low as 0.5 or 0.01 ppm, respectively. Based on the conventional decarboxylation reaction, a simple and sensitive SERS method was proposed for the detection of pectin, which shows potential for practical application.
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Frontiers in Nutrition
·
2022
·
IF 6.59
Hong Tao,Zhao Jiaying,Yin Junyi,Nie Shaoping,Xie Mingyong
Tea is a popular beverage with a long history of safe and healthy use. Tea polysaccharide is a bioactive component extracted from tea, which has attracted more and more attention in recent decades. In this article, an acidic polysaccharide Gougunao tea polysaccharide (GPS) was isolated from Gougunao green tea by hot water extraction and ethanol precipitation. After purification by a diethylaminoethyl (DEAE) Sepharose Fast Flow column and a Sephacryl S-400 column, several homogalacturonan (HG) and rhamnogalacturonan-I (RG-I) fractions were obtained. Fraction GPS2b with the highest yield was selected for structural characterization by methylation and nuclear magnetic resonance (NMR) analysis. GPS2b was found to be an HG-type pectic polysaccharide (degree of methyl esterification [DE], 51.6%) with low molecular weight (Mw, 36.8 kDa). It was mainly composed of →4)-α-GalpA- (1→ and →4)-α-GalpA-6-OMe-(1→. In addition, a minor highly branched RG-I domain was identified in this fraction. The investigation of structural features of tea polysaccharides can provide insights to understand their structure-bioactivity relationship.
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JOURNAL OF POLYMER RESEARCH
·
2022
·Vol. 29
·期 6
·1-11
·
IF 3.061
Huang Tuankang,Qin Youlin,Li Mingchao,Gao Shanjun,Shen Chunhui
Deacetylated konjac glucomannan (DKGM)/calcium pectin (Ca-PEC) composite films were prepared by calcium hydroxide to realize deacetylation of konjac glucomannan (KGM) and the crosslinking of pectin at the same time. Fourier transform infrared spectroscopy (FTIR) indicates that there are intermolecular hydrogen bond and crosslinking structure between DKGM and Ca-PEC. The physical properties of the composite films are characterized by mechanical properties, water vapor permeability (WVP), swelling degree (SD), water solubility (WS), thermogravimetry (TG) and Opacity. The results exhibit that deacetylation of KGM and crosslinking structure of pectin reduce the swelling degree and water solubility of the films, as well as improve its thermal stability and tensile strength. These are attributed to enhanced intermolecular interactions, more compact entanglement of molecular chains and more solid spatial network structures. When pectin content is 40%, the combination property of DKGM / Ca-PEC composite film is the best.
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Foods
·
2021
·Vol. 10
·期 12
·3127
·
IF 4.35
Risi Wang,Ya Li,Xixiang Shuai,Jun Chen,Ruihong Liang,Chengmei Liu
Traditional aerogels lack specific functional groups for the adsorption of Pb2+, which results in a low adsorption capacity and limits the application scope. Novel porous pectin-based aerogels (PPEAs) were prepared by incorporating polyethylenimine (PEI) using ethylene glycol diglycidyl ether (EGDE) as a cross-linker for the removal of Pb2+from water. The cross-linking mechanism, morphology, mechanical strength, thermal stability, adsorption properties, and mechanism of the aerogels were investigated. The aerogels possessed several desirable features, such as a large maximum Pb2+adsorption capacity (373.7 mg/g, tested at pH 5.0), ultralight (as low as 63.4 mg/cm3), high mechanical strength (stress above 0.24 MPa at 50% strain), and easy recyclability. Meanwhile, the equilibrium adsorption data was well described by the Langmuir–Freundlich (Sips) model and the kinetic adsorption process was well fitted using the pseudo-second-order model. The donor groups, such as -NH2, and oxygen-containing functional groups were responsible for the Pb2+adsorption, which was confirmed by the FTIR and XPS analysis. The excellent characteristics mean that PPEAs are highly effective adsorbents in the remediation of lead-containing wastewater.Keywords:pectin;PEI;EGDE;aerogel;Pb2+;adsorption
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Foods
·
2023
·Vol. 12
·期 10
·2042
·
IF 5.2
Lanlan Hu,Yangyang Jia,Xiaoxiao Zhang,Yajie Zhang,Meizhu Dang,Chunmei Li
The present study aimed to evaluate the capability of persimmon pectin (PP) as a stabilizer for acid milk drinks (AMDs) compared with commercial high-methoxyl pectin (HMP) and sugar beet pectin (SBP). The effectiveness of pectin stabilizers was assessed by analyzing particle size, micromorphology, zeta potential, sedimentation fraction, storage, and physical stability. Results of CLSM images and particle size measurements showed that PP-stabilized AMDs had smaller droplet sizes and more uniform distributions, indicating better stabilization potential compared with the HMP- and SBP-stabilized AMDs. Zeta potential measurements revealed that the addition of PP significantly increased the electrostatic repulsion between particles and prevented aggregation. Moreover, based on the results of Turbiscan and storage stability determination, PP exhibited better physical and storage stability compared with HMP and SBP. The combination of steric repulsion and electrostatic repulsion mechanisms exerted a stabilizing effect on the AMDs prepared from PP. Overall, these findings suggest that PP has promising potential as an AMD stabilizer in the food and beverage industry.Keywords:persimmon pectin;stability;acidified milk drinks;natural ingredients
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2023
·
IF 5.8
Danyang Chen,Xiangbo Ma,Jingjing Zhu,Yong Wang,Shuai Guo,Jianglei Qin
Self-healing hydrogels have shown great application potential in drug delivery for anti-tumor therapy and tissue engineering. In this research, Doxorubicin (DOX) was coupled onto the oxidized pectin (pec-Ald) to prepare DOX grafted pec-AD and used to fabricate self-healing hydrogel for lung cancer therapy combined with novel herbal medicine extract limonin targeting lung cancer cells. The hydrogel was prepared with P(NIPAM 195 - co -AH 54 ) cross-linking and the hydrazone bond cross-linked hydrogel showed good mechanical property and self-healing behavior. With pectin composition , the hydrogel was still biodegradable catalyzed by enzyme and in vivo. The hydrogel formed fast fit for injectable application and the hydrogel itself showed moderate lung cancer inhibition activity. With limonin loading, the hydrogel showed synergistic lung cancer therapy with the tumor growth greatly inhibited. The covalent coupling of DOX and loaded limonin in the hydrogel decreased in vivo toxicity and the hydrogel degraded on time. With biodegradability and improved lung cancer therapy efficiency, this DOX grafted self-healing hydrogel could find great potential application in cancer therapy in near future.
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ENERGY & FUELS
·
2021
·
IF 3.605
Hao Xu,Shanshan Xiong,Yuan Zhao,Lingjun Zhu,Shurong Wang
Furfural is an important platform chemical that can be produced by dehydration of pentose, which is an important building block of lignocellulosic biomass. In this study, xylose was chosen as the reaction substrate, and a carbon-based solid acid prepared from pectin was used as the catalyst in a mixed solvent consisting of water and γ-valerolactone (GVL). FTIR results showed that the sulfonated catalyst had typical characteristic peaks of sulfonic groups, while the results based on NH3-TPD and elemental analysis showed that the sulfonation temperature changed the surface acid concentration and acid site intensity of the catalyst. A furfural yield of 80.4% and a xylose conversion of 100% were obtained at 170 °C for 60 min. The conversions and product yields were influenced by the reaction temperature, reaction time, solvent composition, and catalyst acidity. Different sulfonation temperatures can change the acidity of the catalyst, which determines the catalytic performance of the catalyst.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2020
·
IF 5.162
Xuan Zhang,Jiawei Lin,Fang Pi,Tao Zhang,Chao Ai,Shujuan Yu
This work aimed to extract gelatinous chicory root pectin (CRP) and evaluated the rheological behavior of the dispersions and gels. CRP was extracted by citric acid (CEP), alkaline (AEP), ammonium oxalate (OEP) and sodium citrate (SEP). The yield, molecular weight (Mw) and the degree of esterification (DE) of pectin samples varied from 8.8 to 14.8% (w/w), 204 to 336 k Da and 4.0 to 47.4%, respectively. AFM studies showed self-organize on mica of CEP, revealing a random coil conformation due to the interaction of multiple branching, whereas, AEP exhibited long linear filamentous structures. The flow behavior study verified the pseudoplastic character of CEP and SEP at 25 °C, while OEP and AEP belonged to dilatant fluid, besides, a closed hysteresis loop was observed when the CEP concentration increased to 1.5%. OEP gel was thermo insensitive and stiff, AEP gel presented most sensitive to calcium ion but more brittle, and SEP was observed a weak syneresis in spite of the poor gelation property. The texture analysis indicated OEP gel had a superior firmness and chewiness. These findings demonstrated that CRP may be attractive as a thickener or gelling agent to modulate textures of sugar-free and calcium content food.
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Materials Research Express
·
2019
·Vol. 6
·期 6
·065405
·
IF 1.449
Qinghong You,Miaomiao Wan,Xiaoxu Fang,Xiulian Yin,Chuping Luo,Xuejuan Zhang
The process of intermittent microwave extraction (IME) pectin from pomelo peels assisted with EDTA was optimized by response surface methodology. Results showed that the optimal process for intermittent microwave extraction of pectin was as follows: 0.25% EDTA, pH 1.0, solid-to-liquid ratio 1:25 g ml−1, extraction time 125 s, microwave power 550 W, the extraction yield of pomelo peels was (38.48 ± 0.28)%. The structure of three kinds of pectin was verified by IR, SEM, XRD, HPLC and the results showed that pectin obtained from IME had a poor solubility but a good emulsifying activity. The IUEP had the highest scavenging rates of DPPH radicals.
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BMC PLANT BIOLOGY
·
2020
·Vol. 20
·期 1
·1-17
·
IF 3.497
Xue Cheng,Guan Si-Cong,Chen Jian-Qing,Wen Chen-Jin,Cai Jian-Fa,Chen Xu
Background Pectin methylesterase (PME) is a hydrolytic enzyme that catalyzes the demethylesterification of homogalacturonans and controls pectin reconstruction, being essential in regulation of cell wall modification. During fruit ripening stage, PME-mediated cell wall remodeling is an important process to determine fruit firmness and softening. Strawberry fruit is a soft fruit with a short postharvest life, due to a rapid loss of firm texture. Hence, preharvest improvement of strawberry fruit rigidity is a prerequisite for extension of fruit refreshing time. Although PME has been well characterized in model plants, knowledge regarding the functionality and evolutionary property of PME gene family in strawberry remain limited.Results A total of 54 PME genes (FvPMEs) were identified in woodland strawberry (Fragaria vesca ‘Hawaii 4’). Phylogeny and gene structure analysis divided these FvPME genes into four groups (Group 1–4). Duplicate events analysis suggested that tandem and dispersed duplications effectively contributed to the expansion of the PME family in strawberry. Through transcriptome analysis, we identified FvPME38 and FvPME39 as the most abundant-expressed PMEs at fruit ripening stages, and they were positively regulated by abscisic acid. Genetic manipulation of FvPME38 and FvPME39 by overexpression and RNAi-silencing significantly influences the fruit firmness, pectin content and cell wall structure, indicating a requirement of PME for strawberry fruit softening.Conclusion Our study globally analyzed strawberry pectin methylesterases by the approaches of phylogenetics, evolutionary prediction and genetic analysis. We verified the essential role of FvPME38 and FvPME39 in regulation of strawberry fruit softening process, which provided a guide for improving strawberry fruit firmness by modifying PME level.
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