JOURNAL OF ANIMAL SCIENCE
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2026
·
IF 3.7
Zheng Jikang,Zhao Junyin,Li Minhao,Cheng Yuqin,Fang Xingqiang,Zhao Le,Zhao Yongju
Goat semen cryopreservation generates excessive reactive oxygen species (ROS), which impair post-thaw quality and physiological function. This study investigated the effects of resveratrol (RES) on cryopreserved goat sperm and the underlying signaling pathway. The results showed that supplementation with 150 μM RES significantly improved post-thaw sperm motility, plasma membrane integrity, and acrosome integrity (P < 0.05), enhanced SOD, CAT, and T-AOC activities, and reduced MDA content and sperm deformity rates (P < 0.05), while higher concentration (200 μM) showed no significant improvement compared to control, suggesting a detrimental effect of excessive RES. Furthermore, RES effectively alleviated cryo-induced ultrastructural damage to sperm. Mechanistically, RES activated AMPK signaling, increased AMPK phosphorylation, and upregulated the expression of SIRT1 and FoxO1 proteins. In contrast, the AMPK inhibitor Compound C partially blocked these beneficial effects. These findings indicate that RES improves the quality and antioxidant capacity of cryopreserved goat sperm by activating the AMPK/SIRT1/FoxO1 pathway, suggesting that RES can serve as a promising antioxidant additive for goat semen cryopreservation.
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REACTIVE & FUNCTIONAL POLYMERS
·
2025
·
IF 5.1
Zhiyong Li,Da Gao,Xiaoge Li,Dingqiang Lu,Jingjing Meng,Kai Guo
Since little attention has been paid to the dual biobased epoxy resin monomers, a novel monomer (ResCMF) from resveratrol and 5-hydroxymethylfurfural was designed for the biobased network fabrication with diaminodiphenyl disulphide (AFD) as a hardener. Comprehensive behaviors of the solvent-free cured networks were investigated. In contrast to the epoxide-amine polymerization in DGEBA/AFD, an additional amine-catalyzed self-polycondensation of ResCMF also occurred during ResCMF/AFD formation. According to the activation energy estimation of the epoxide-amine reaction, the 44AFD hardener is more reactive than the 22AFD. For ResCMF/AFD, the initial epoxide-amine polymerization allowed the network with a moderate T g of about 107.2/109.3 °C, while the self-polycondensation showed high T g values at 194.6 /188.6 °C. Moreover, ResCMF/22AFD outperformed ResCMF/44AFD in terms of storage modulus (4.55 > 3.21 GPa), larger than that of the DGEBA/AFD. It demonstrated outstanding tensile strength (85.1 ± 3.5 MPa) and high flexural strength (118.4 ± 18.9 MPa) as well. Meanwhile, comparable impact and lap shear strengths were observed for all networks. Interestingly, high-performance shape-memory ResCMF/AFD at 150 °C enabled the sustainable reprocessing. Furthermore, in general, the thermosets showed good solvent resistance. Nonetheless, the sustainable post-treatment via DMF-induced solvolysis enables ResCMF/AFD degradation. As a result, the dual-cured ResCMF/AFD networks provide good overall performance for their applications in the future.
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FOOD CHEMISTRY
·
2025
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IF 9.8
Xiang Luo,Hongze Wu,Zhe Jiang,Yanyan Zhou,Runpu Shen,Zhennan She,Tao Cai,Meiyang Xi,Kewu Zhu
Enhancing the stability, antioxidant capacity, and bioaccessibility of resveratrol (Res) is critical for its functional food application. Herein, we developed a novel antioxidant emulsifier, polylysine-polyglyceryl-10-decanoate (PL-PG10-C10), synthesized via periodate oxidation and Schiff-base coupling. Compared with PG10-C10, PL-PG10-C10 reduced the critical micelle concentration (38.2 vs. 4.3 μg/mL) and produced Res nanoemulsions with high encapsulation efficiency (90.4 %) and superior stability across pH 1–13 and 0–500 mM NaCl. PL-PG10-C10-Res nanoemulsions showed stronger radical scavenging (94.0 % vs. 71.7 %) and delayed lipid oxidation over 21 days at 55 °C. Simulated digestion confirmed excellent gastric stability and improved intestinal delivery, achieving 91.3 % bioaccessibility versus 63.7 % for PG10-C10-Res nanoemulsions. Release kinetics followed mixed mechanisms, showing combined diffusion- and erosion-controlled behavior, with first-order, Higuchi, and Korsmeyer-Peppas models providing complementary fits, reflecting the dynamic interplay of diffusion, interfacial relaxation, and enzymatic digestion. These findings underscore PL-PG10-C10 as a promising platform for oral delivery of lipophilic bioactives in functional foods.
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BIOMATERIALS
·
2025
·
IF 12.9
Lamei Liu,Min Zhang,Yan Zhou,Lin He,Mingjian Chen,Yuchen Wu,Guanyu Yin,Hui He,Lanlin Qi,Bin Zhang,Xiaoxiao He,Kemin Wang
Osteoarthritis (OA) is a complex and multifactorial joint disorder. Currently, there are few therapies to slow the progression of OA. Combining symptomatic slow-acting drugs and reactive oxygen species (ROS) scavenging reagents in the inflamed joint shows a promising therapeutic approach for OA. Nonetheless, the short residence time of free drugs in the joint cavity hinders their further application. Herein, an injectable composite of hydrogel and chondroitin sulfate@resveratrol liposome package (Gel/Lip@Res + Chs) was developed to improve drug residency ability and enable the simultaneous delivery of hydrophilic Chs and hydrophobic Res in the joint cavity. This injectable hydrogel, based on phenylboronic acid modified hyaluronic acid (HAPBA) and polyvinyl alcohol (PVA), provided ROS-responsive decomposition property and sustained drug release behavior, thereby effectively scavenging ROS, promoting macrophage repolarization, and decreasing the expression of inflammatory mediators and matrix-degrading enzymes in vitro . As a proof of concept, the in vivo therapeutic efficacy of Gel/Lip@Res + Chs was further examined in both monoiodoacetic acid (MIA)-induced and surgical anterior cruciate ligament transection (ACLT)-induced OA mouse models. Benefiting from the extended residence time of loaded drugs in the joint cavity, the Gel/Lip@Res + Chs treatment could effectively suppress cartilage degeneration and increase the synthesis of the cartilage matrix, thus slowing the advancement of OA. The present study provides a reference for improving drug retention and holds great potential for treating OA-related diseases.
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CHEMICAL ENGINEERING SCIENCE
·
2026
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IF 5.1
Jiajia Ren,Guanlin Li,Qiwen Yong,Minjia Meng
Resveratrol (RES), a high-value bioactive compound, faces extraction limitations in conventional separation processes. This study reported a sustainable free-standing molecularly imprinted membrane (FMIM) fabricated through photoinitiated click polymerization on functionalized MnO 2 nanowires for simultaneous selective RES separation and soil quality enhancement. The FMIM was synthesized via UV-induced thiol-ene crosslinking, yielding a flexible, self-supported membrane with high-density and uniformly distributed recognition sites. The resulting MnO 2 -based MIPs exhibited an adsorption capacity of 48.60 mg g −1 for RES, outperforming the majority of common MIPs reported in the literature. Under cross-flow conditions, the FMIM exhibited high selectivity for RES over its structural analog caffeic acid (CA), achieving a cross-flow selectivity factor ( β ) of 9.05 compared to 6.07 for a PVDF-based MIM (PMIM), attributable to its localized high-density binding sites in FMIM. Importantly, the FMIM displayed dual functionality: efficient molecular recognition and soil remediation capability. Furthermore, the FMIM exhibited dual functionality by also contributing to soil health. In a 30-day soil incubation experiment, the MnO 2 component was found to mitigate nutrient loss and support microbial activity, resulting in a non-significant reduction in the soil multifunctionality index (SMI) of only 4.75% for the FMIM, compared to a significant reduction of 15.63% for the PMIM. This work provided a green strategy for designing multifunctional separation materials that align with sustainable chemistry engineering principles, offering potential applications in pharmaceutical purification and environmentally compatible agriculture.
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BMC Complementary Medicine and Therapies
·
2023
·Vol. 23
·期 1
·1-11
·
IF 3.9
Cai Weiye,Sun Bin,Song Chao,Liu Fei,Wu Zhengliang,Liu Zongchao
Background In mouse, it was discovered that resveratrol (Res) enhanced osteoporosis (OP) by boosting osteogenesis. Besides, Res can also have an impact on MC3T3-E1 cells, which are crucial for the control of osteogenesis and thus increase osteogenesis. Although some articles have discovered that Res enhanced autophagy to promote the value-added differentiation of MC3T3, it is unclear exactly how this affects the process of osteogenesis in mouse. Therefore, we will show that Res encourages MC3T3-E1 proliferation and differentiation in mouse pre-osteoblasts and further investigate the autophagy-related mechanism for this impact.Methods (1) MC3T3-E1 cells were separated into blank control group and various concentrations (0.01, 0.1, 1, 10, 100µmol/L) of group in order to determine the ideal Res concentration. In the Res group, Cell Counting Kit-8 (CCK-8) was used to measure the proliferation activity of pre-osteoblasts in mice in each group after resveratrol intervention. Alkaline Phosphatase (ALP) and alizarin red staining were used to gauge the degree of osteogenic differentiation, and RT-qPCR was used to measure the expression levels of Runx2 and OCN in the osteogenic differentiation ability of the cells. (2) In the experiment, four groups were set up: the control group, 3MA group, Res group, and Res + 3MA group. To examine cell mineralization, ALP and alizarin red staining were utilized. RT-qPCR and Western blot detection of cell autophagy activity levels and osteogenic differentiation capacity in each group following intervention.Results (1) Resveratrol might increase the number of mice pre-osteoblast, with the impact being most pronounced at 10µmol/L (P < 0.05). The nodules developed substantially more often than in the blank control group, and Runx2 and OCN expressions significantly increased (P < 0.05). (2) In contrast to the Res group, after 3MA purine blocked autophagy, the Res + 3MA group’s alkaline phosphatase staining and the development of mineralized nodules were reduced. Runx2, OCN, LC3II / LC3I expression decreased, p62 expression increased (P < 0.05).Conclusion The present study partially or indirectly demonstrated that Res may, through increased autophagy, induce osteogenic differentiation of MC3T3-E1 cells.
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Foods
·
2022
·Vol. 11
·期 22
·3741
·
IF 5.561
Ying Sun,Yang Yang,Lili Zheng,Xiaoyan Zheng,Dao Xiao,Shenwan Wang,Zhengke Zhang,Binling Ai,Zhanwu Sheng
Ultrasonic treatment combined with resveratrol modification was used to improve banana starch’s solubility, thermal stability, and digestion resistance. The solubility and freeze-thaw stability of the modified starch complex significantly increased. The oil-absorption capacity increased by 20.52%, and the gelatinization temperatures increased from 64.10–73.92 °C to 70.77–75.83 °C. The storage modulus (G′) and loss modulus (G″) increased after ultrasound and resveratrol treatment, and the proportion of viscosity was increased after composition with resveratrol. Additionally, the in vitro digestibility decreased from 44.12% to 40.25%. The modified complexes had release-control ability for resveratrol. X-ray diffraction (XRD) and Fourier transform infrared (FT-IR) spectroscopy demonstrated that complex structures became more compact and organized, whereas crystalline patterns were unchanged. Scanning electron microscopy (SEM) showed that the resveratrol modification caused physical change on the granular surface by creating pores and fissures. The findings can help develop antioxidant functional foods using banana starch.Keywords:starch modification;thermal stability;rheological properties;crystallinity;morphology
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Foods
·
2023
·Vol. 12
·期 13
·2436
·
IF 5.2
Duoduo Li,Zihao Wei,Xiaolong Li
Hollow nanoparticles have attracted extensive attention due to their advantages such as high loading capacity and superior stability. However, the complexity of the preparation process and harmfulness of the used raw materials have limited their application in the food field. Based on this, hollow gliadin nanoparticles (HGNPs) were developed using a Na2CO3sacrificial template method. The findings of this study suggested that HGNPs could be regarded as a delivery system for resveratrol (Res) and they exhibited excellent delivery performance. Compared with solid gliadin nanoparticles (SGNPs), the HGNPs displayed smaller particle sizes, better physical stability, higher encapsulation efficiency, stronger resistance to ultraviolet light and a more sustained release of Res in the gastrointestinal tract. This work is of practical significance for the development and utilization of protein-based nanoparticles with hollow structures as a delivery system for sensitive bioactives.Keywords:gliadin nanoparticle;sacrificial template;hollow structure;resveratrol;delivery system;sustained release
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INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
·
2023
·Vol. 24
·期 13
·10914
·
IF 5.6
Minghui Cai,Haijuan Sun,Yujia Huang,Haixu Yao,Chen Zhao,Jiao Wang,Hui Zhu
Resveratrol performs a variety of biological activities, including the potential regulation of autophagy. However, it is unclear whether resveratrol protects against luteal dysfunction and whether autophagy involves the regulation of resveratrol. This study aims to investigate whether resveratrol can regulate autophagy to resist H2O2-induced luteinized granulosa cell dysfunction in vitro. Our results showed that resveratrol can enhance cell viability, stimulate the secretion of progesterone and estradiol, and resist cell apoptosis in H2O2-induced luteinized granulosa cell dysfunction. Resveratrol can activate autophagy by stimulating the expression of autophagy-related genes at the transcriptional and translational levels and increasing the formation of autophagosomes and autophagolysosomes. Rapamycin, 3-methyladenine, and bafilomycin A1 regulated the levels of autophagy-related genes in H2O2-induced luteinized granulosa cell dysfunction and further confirmed the protective role of autophagy activated by resveratrol. In conclusion, resveratrol activates autophagy to resist H2O2-induced oxidative dysfunction, which is crucial for stabilizing the secretory function of luteinized granulosa cells and inhibiting apoptosis. This study may contribute to revealing the protective effects of resveratrol on resisting luteal dysfunction from the perspective of regulating autophagy.Keywords:autophagy;resveratrol;corpus luteum;luteinized granulosa cell
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Drug Design Development and Therapy
·
2022
·
IF 4.319
Liang Wang,Qiushi Li,Haibo Yan,Guangjun Jiao,Hongliang Wang,Hai Chi,Hongming Zhou,Lu Chen,Yu Shan,Yunzhen Chen
Purpose Glucocorticoids are used for the treatment of inflammatory diseases, but glucocorticoid treatment is associated with bone damage. Resveratrol is a phytoalexin found in many plants, and we investigated its protective role on dexamethasone-induced dysfunction in MC3T3-E1 cells and primary osteoblasts.Materials and Methods MC3T3-E1 cells and primary osteoblasts were treated with dexamethasone in the presence/absence of different doses of resveratrol for 24 or 48 h. Then, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium (MTT) and lactate dehydrogenase (LDH) assays were used to evaluate cell viability. Apoptosis was analyzed by a flow cytometry. An alkaline phosphatase (ALP) activity assay and Alizarin Red S staining were used to study osteoblast differentiation. Expression of osteoblast-related genes was measured by real-time reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The AMP-activated protein kinase (AMPK) signaling pathway and mitochondrial expression of superoxide dismutase were evaluated by Western blotting. Intracellular reactive oxygen species (ROS), adenosine triphosphate (ATP) content, mitochondrial-complex activity, and mitochondrial DNA content were measured to evaluate mitochondrial function.Results Resveratrol induced the proliferation and inhibited apoptosis of osteoblasts in the presence of dexamethasone. Resveratrol increased the ALP activity and mineralization of osteoblasts. Resveratrol also attenuated dexamethasone-induced inhibition of mRNA expression of osteogenesis maker genes, including bone morphogenetic protein-2, osteoprotegerin, runt-related transcription factor-2, and bone Gla protein. Resveratrol alleviated dexamethasone-induced mitochondrial dysfunction. Resveratrol strongly stimulated expression of peroxisome proliferator–activated receptor-γ coactivator 1α and sirtuin-3 genes, as well as their downstream target gene superoxide dismutase-2. Resveratrol induced phosphorylation of AMPK and acetyl-CoA carboxylase (ACC). Blockade of AMPK signaling using compound C reversed the protective effects of resveratrol against dexamethasone.Conclusion Resveratrol showed protective effects against dexamethasone-induced dysfunction of osteoblasts by activating AMPK signaling.
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Tian Li,Qin Guo,Yang Qu,Hongzhi Liu,Li Liu,Yu Zhang,Qiang Wang
The unanticipated inhibitory effect of trans -resveratrol ( trans -Res) on the formation of trans fatty acids (TFAs) by thermal isomerization of peanut oil (PO) and its mechanism were investigated by experiment and density functional theory. Results showed that trans -Res inhibited the amount and formation rate of TFAs. t rans -Res first inhibited the formation of C18:2, then C18:1, by the mechanism of proton transfer isomerism. The most active reaction site of trans -Res (4′-OH free radical) preferentially combined with the OOL-L-C11• (di-allyl) and then with OOL-O-C11• (mono-allyl) allyl groups in PO, resulting in the higher reaction energy barrier of speed control steps in OOL-L (transition state 1) and OOL-O (transition state 2), and the lower reaction rate of OOL-L and OOL-O (both decreased by 1–10 3 times), to reduce the formation of TFAs. Our study provided a theoretical foundation for the precise regulation of natural hydroxy compound to TFAs in oil.
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BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
·
2022
·
IF 3.322
Ting Zhou,Bao Yang
Prenylated resveratrols are drug candidates presented in many natural sources. They possess multiple pharmacological activities. Due to the limited abundance in nature and disadvantage of chemical synthesis, it is required to produce prenylated resveratrols by biological strategy. In this work, a prenyltransferase iacE from Pestalotiopsis fici was prepared by heterologous overexpression. It could catalyze 2- C -prenylation of resveratrol efficiently. Dimethylallyl diphosphate (DMAPP) and geranyl diphosphate (GPP) could serve as prenyl donor. Glu97, Thr114 and Tyr413 were proved to be important residues in the active site by site-directed mutagenesis. The product was purified and identified to be 2- C -prenyl resveratrol by NMR . 2- C -prenyl resveratrol exerted neuroprotective activity through inhibiting ROS overproduction and improving the activities of antioxidant enzymes in HT22 cell. The genes involved in antioxidant defense were regulated, including upregulated CAT , SOD2 , NFE2L2 , GPx4 , and downregulated PTGS2 and ACSL4 . These results supplied a novel technique for prenylated resveratrols production.
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危险品化学品经营许可证（带存储）