JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2015
·
IF 2.729
Jie Zhou,Shanshan Chen,Fang Sun,Pei Luo,Qiuzheng Du,Suzhen Zhao
In recent years, the study of chiral compounds in vivo has received much attention. In this study, a novel method based on high performance liquid chromatography (HPLC) coupled with chemiluminescence (CL) detection was developed for the separation of tryptophan (Trp) enantiomers. o -Phthalaldehyde and N -acetyl- l -cysteine were used as chiral derivatization reagents for Trp before it can be detected by HPLC-CL method. The separation was carried out on an ODS column using a mobile phase composed of methanol—0.01 mol/L phosphate buffer (40/60, v/v). Under the optimum conditions, satisfactory results were obtained, including complete separation, good relative standard deviations and low detection limits. The applicability of the proposed method has been validated by determining Trp in biological samples. Linear responses ( r > 0.9990) were observed over the range of 2.5 × 10 −7 to 1.2 × 10 −5 g/mL of Trp enantiomers, with quantitation limit of 2.5 × 10 −7 g/mL. The assay method shows good specificity to Trp enantiomers, and thus it will have great potential application in clinical diagnosis. The mean extraction efficiency of Trp enantiomers in mice plasma samples were 98.48% and 97.40%, respectively. The mean relative standard deviation (RSD) of Trp enantiomers were <3%.
研究领域:
分析化学
·
生物化学
·
药物分析
✓ 已验证
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2011
·
IF 2.48
Yunchang Fan,Sheli Zhang,Jichuan Kong,Juan Miao
In this work, the interaction of an ionic liquid (IL) 1-octyl-3-methylimidazolium chloride ([Omim]Cl) and L -tryptophan was investigated with fluorescence spectroscopic method. The quenching mechanism and binding characteristics of [Omim]Cl with L -tryptophan at different temperatures were studied from fluorescence measurement. The thermodynamic parameters such as free energy change (Δ G ), enthalpy change (Δ H ) and entropy change (Δ S ), were calculated by thermodynamic equations. The experimental results indicated that [Omim]Cl and L -tryptophan had weak interaction and the mechanism of quenching belonged to static quenching. Hydrogen bonds and van der Waals interactions played important roles in the binding of [Omim]Cl with L -tryptophan.
研究领域:
生物化学
·
分子生物学
✓ 已验证
DOI
高影响因子
npj Science of Food
·
2026
·
IF 9.7
Lingnv Yao,Siyuan Xie,Shuobo Zhang,Furong Ba,Huijuan Jing,Bo Qiu,Lvwan Xu,Shengyi Han,Hang Xiao,Ming Zhao,Xiaowei Shi,Lanjuan Li,Mingfei Yao
Premature ovarian insufficiency (POI) threatens women’s reproductive health. Emerging evidence implicates the gut-ovary axis in POI pathogenesis. This study explored the probiotic Lactobacillus salivarius Li01 in a Tripterygium wilfordii glycosides-induced POI mouse model. Li01 effectively mitigated POI progression by restoring hormone levels, increasing functional follicle numbers, and improving ovarian antioxidant function. These beneficial effects were primarily mediated through modulation of the gut microbiota and host tryptophan metabolism. Notably, a tryptophan-enriched diet alone did not improve POI. When combined with Li01, it synergistically amplified the kynurenine pathway. This shift promoted ovarian NAD⁺ production by upregulating the levels of NAMPT, while attenuating NAD⁺ depletion through downregulation of CD38 expression. The elevated NAD⁺ levels subsequently activated SIRT1 expression, stimulated the synthesis of SOD and other antioxidants, and inhibited the Caspase-1/IL‑1β inflammasome axis. These findings highlight Li01 as a potential POI therapy through microbiota-metabolic regulation, offering new mechanistic insights into gut-ovarian interactions.
✓ 已验证
DOI
PubMed
高影响因子
SOLID STATE SCIENCES
·
2026
·
IF 3.6
Chi Qu,Chenqiu Dai,Kaiyin Shi,Ji Zhu,Fangyuan Liu,Xiuwen Zhu,Cheng Guo,Jianqiang Wang
A novel ferulic acid-tryptophan single-bridge-linked organosilicon precursor (FA-Trp-OSP) was synthesized via a multi-step reaction. • A ferulic acid-tryptophan bridged periodic mesoporous organosilica (FA-Trp-PMS) was synthesized via a sol-gel strategy for the first time. • The incorporation of dual antioxidant functional groups significantly enhances the radical scavenging performance, showing efficient and selective activity toward ABTS.• + , •OH and O 2 • - radicals. • A synergistic antioxidant mechanism based on hydrogen atom transfer and electron transfer pathways is proposed. Oxidative stress caused by excessive free radicals is a major contributor to various diseases. To develop novel antioxidant, a ferulic acid-tryptophan organosilane precursor (FA-Trp-OSP) was synthesized through a multi-step organic synthesis process. Subsequently, it was co-condensed with tetraethyl orthosilicate (TEOS) to fabricate a functionalized mesoporous silica material (FA-Trp-PMS). An optimal precursor loading of 30% maintained a well-ordered mesoporous structure while maximizing the density of functional groups. FA-Trp-PMS displayed superior radical scavenging activity against ABTS• + , •OH, and O 2 • - , attaining scavenging rates of 82%, 92%, and 49% at concentrations of 1.5, 2.0, and 5.0 mg/mL, respectively. The material significantly outperformed the corresponding single-component systems and physical mixture system, exhibiting enhanced antioxidant performance that may be associated with cooperative interactions between ferulic acid and tryptophan functionalities. FA-Trp-PMS also exhibited rapid kinetics (reaching equilibrium within 3-10 min) and good stability over a temperature range of 5-40 °C and within the investigated pH range. This work provides a promising strategy for the development of high-performance antioxidant materials with multi-radical scavenging activity and good environmental stability for applications in food preservation, cosmetic stabilization, and biomedicine. Download: Download high-res image (271KB) Download: Download full-size image
✓ 已验证
DOI
高影响因子
Journal of Stroke & Cerebrovascular Diseases
·
2025
·
IF 1.8
Chuzheng Pan,Feng Chen,Yan Yan,Haiwen Li,Chengfeng Qiu
Objective: Ischemic stroke is among the leading causes of mortality and long-term disability worldwide. A growing body of evidence indicates alterations in metabolite levels and enzyme activities associated with the conversion of tryptophan (TRP) throughout the course of cerebral ischemia. In this study we aim to explore the potential relationship between TRP metabolism and clinical prognosis in acute ischemic stroke (AIS) patients of mainland China. Materials and methods : Blood samples were obtained from a cohort of 304 patients diagnosed with AIS. The concentrations of ten TRP metabolites were quantified utilizing liquid chromatography-tandem mass spectrometry (LC-MS/MS). Stroke severity was evaluated upon admission using the National Institutes of Health Stroke Scale (NIHSS). A poor functional outcome was defined as a modified Rankin scale (mRS) > 3, whereas a good functional outcome was defined by mRS ≤ 3 at 3 months post-stroke. LASSO regression and random forest algorithms were then employed to identify key TRP metabolism parameters associated with prognosis. Results : Following the optimization of variable selection through Lasso regression, a prognostic risk model with 7-factors related to AIS was constructed, yielding an AUC of 0.917. Subsequently, a random forest analysis was conducted to establish an 11-factor prognostic risk model, which demonstrated an enhanced AUC of 1.000. Ultimately, three robust parameters related to TRP metabolism were identified. Multivariable logistic regression analysis, adjusted for covariates, revealed that TRP (odds ratio [OR] = 0.46, 95% confidence interval [CI]: 0.26 - 0.76, p = 0.004), the kynurenine (KYN)/TRP ratio (OR = 2.06, 95% CI: 1.23 - 3.60, p = 0.008), and the kynurenic acid (KYNA)/TRP ratio (OR = 2.15, 95% CI: 1.23 - 4.12, p = 0.014) were independently associated with poor functional prognosis. Conclusions : The results of this study indicate that TRP metabolism is associated with the severity and prognosis of AIS. The TRP, KYN/TRP ratio and KYNA/TRP ratio may serve as potential biomarkers for 3-month prognostic evaluation.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2025
·
IF 4.1
Junyao Li,Nan Shi,Chen Jin,Laidi Xu,Yong Kong
Au nanoparticles (AuNPs) are adsorbed onto the surface of multi-hole polydopamine (mPDA) nanospheres, which are utilized for the covalent bonding of L-cysteine (L-Cys) as the chiral source. The obtained mPDA@AuNPs/L-Cys is applied in the spectroscopic and electrochemical discrimination of tryptophan (Trp) enantiomers. Due to the same rotatory direction of L-Cys and L-Trp, L-Trp is selectively combined with the mPDA@AuNPs/L-Cys, and thus the chiral discrimination of the Trp enantiomers can be successfully achieved. Chiral solid polydopamine (sPDA) nanospheres are also synthesized and used for the chiral discrimination of the Trp enantiomers, while the discrimination ability of the chiral sPDA is significantly lower than that of the chiral mPDA. The improved discrimination ability of the chiral mPDA can be ascribed to its unique multi-hole structure, which is in favour of combining more amount of AuNPs and L-Cys.
研究领域:
纳米医学
·
分析化学
·
生物传感器
·
手性化学
·
分子识别
✓ 已验证
产品关联
DOI
高影响因子
ANALYTICA CHIMICA ACTA
·
2024
·
IF 5.7
Qi Wu,Yuan Chen,Yan-Li Wang,Ji-Ying Song,Hai-Tao Lv,Ya-Ming Sun
Background Chirality is an essential property of nature. Chiral recognition is of great significance to life sciences, pharmaceutical industry, food analysis, and so on. Chiral carbon dots (CCDs), as green nanomaterials, have great prospects in chiral sensing. However, CCDs with enantioselectivity for tryptophan (Trp) enantiomers are scarce. Moreover, most chiral sensing platforms depend on the difference of fluorescence intensity at the same emission wavelength to identify enantiomers, it is still a challenge to distinguish enantiomers by the positions of fluorescent emission peaks. Results Novel CCDs with specific chiral recognition ability for Trp enantiomers are synthesized using l -lysine and l -cysteine as precursors. The CCDs have two fluorescent emission peaks at 390 nm and 450 nm. Interestingly, the fluorescence intensity of CCDs at 390 nm enhances obviously on the addition of L-Trp, while it enhances slightly at 450 nm in the presence of D-Trp. This chiral sensing system not only can identify Trp enantiomers according to fluorescence intensity, but also achieves the distinguishment depending on emission wavelength. The enantioselectivity (I L /I D ) reaches 4.5 when the concentration of Trp enantiomer is 1 mM. This chiral sensing platform not only can be used for quantitative analysis of D-Trp and L-Trp, but also can be used for determining the enantiomeric excess of racemates. The chiral recognition mechanism is investigated by molecular simulation. It is found that L-Trp has higher binding energy with CCDs. Significance This work presents a novel kind of CCDs with special chiral recognition performance for Trp enantiomers, and opens the door to identify chiral isomers according to wavelength difference, which has profound significance for the development of chiral sensing platforms, and may provide inspirations for the design of novel CCDs with excellent chiral recognition performance.
研究领域:
分析化学
·
生物传感
·
纳米材料科学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ANALYTICAL BIOCHEMISTRY
·
2024
·
IF 2.6
Mengyu Jiang,Li Li,Yuan Jin,Liuliu Lu,Zhenchen Lu,Wangjie Lv,Xiaoqun Wang,Lei Di,Zhicheng Liu
Sepsis is a life-threatening condition characterized by organ dysfunction resulting from a dysregulated host response to infection. Dysregulated tryptophan (TRP) metabolites serve as significant indicators for endogenous immune turnovers and abnormal metabolism in the intestinal microbiota during sepsis. Therefore, a high coverage determination of TRP and its metabolites in sepsis is beneficial for the diagnosis and prognosis of sepsis, as well as for understanding the underlying mechanism of sepsis development. However, similar structures in TRP metabolites make it challenging for separation and metabolite identification. Here, high-performance liquid chromatography coupled with a diode array detector (HPLC-DAD) was developed to determine TRP metabolites in rat serum. The first-order derivative spectrophotometry of targeted metabolites in the serum was investigated and proved to be promising for chromatographic peak annotation across different columns and systems. The established method separating the targeted metabolites was optimized and validated to be sensitive and accurate. Application of the method revealed dysregulated TRP metabolites, associated with immune disorders and NAD + metabolism in both the host and gut flora in septic rats. Our findings indicate that the derivative spectrophotometry-assisted method enhances metabolite identifications for the chromatographic systems based on DAD detectors and holds promise for precision medicine in sepsis.
研究领域:
分析化学
·
免疫学
·
代谢组学
·
微生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food Frontiers
·
2024
·
IF 7.4
Hang Yu,Jingmin Fan,Rui Xue,Jiawei Guan,Fangyuan Tian,Yu Xu,Linyun He,Ji Liu,Zhenzhu Du,Daqun Chen,Wei Yu,Wen Chen,Bo Han
Alhagi honey (AH) is produced in arid and hot areas of Central Asia, and its polysaccharides (AP) are widely known for their activity in the treatment of intestinal diseases such as diarrhea. However, the therapeutic potential and mechanism of AP in ulcerative colitis (UC) remain unclear. Here, AH polysaccharide-2 (AP2), a polysaccharide with the highest content in AP, was isolated and evaluated for its effects on dextran sulfate sodium (DSS)-induced UC in mice. AP2 was found to alleviate UC symptoms and regulate gut microbiota dysbiosis by decreasing Helicobacter levels and increasing Lactobacillus levels. Analysis of PICRUSt2 predicted that AP2 may regulate carbohydrate and amino acid metabolism, and metabolomic analysis confirmed that AP2 promotes the metabolism of tryptophan to produce kynurenic acid (kyna). Moreover, kyna acted as an aryl hydrocarbon receptor (AhR) ligand, which activated AhR to increase the expression of the tight junction proteins claudin-1 and occludin. Interestingly, AP2 showed similar effects in protecting the intestinal barrier and alleviating colitis as the AhR agonist 6-formylindolo[3,2- b ]carbazole, and the AhR antagonist CH223191 partially blocked the therapeutic effect of AP2 in UC mice, indicating that the anti-UC effect of AP2 was AhR dependent. These findings demonstrate that AP2 alleviates UC by regulating the gut microbiota and promoting tryptophan metabolism to generate kyna-activated AhR. The insights gained from this study could help in the future development of AP2 as a drug candidate or functional food for the treatment of UC.
研究领域:
胃肠病学
·
免疫学
·
微生物学
·
药理学
✓ 已验证
产品关联
DOI
高影响因子
EUROPEAN JOURNAL OF PHARMACOLOGY
·
2025
·
IF 4.7
Dan-dan Zhi,Xi-ying He,Lin-fu Yang,Yun-fei Xue,Yi-qiu Liu,Dan Yue,Yi-nan Feng,Kun Dong,Ya-kai Tian
Royal jelly acid is a unique unsaturated fatty acid isolated from royal jelly. Recently, royal jelly acid was proposed to have potential therapeutic effects on hyperlipidemia. However, its effect on hyperlipidemia and the underlying molecular mechanism remains unclear. Therefore, in this study, we analyzed the mechanism of anti-hyperlipidemia of royal jelly acid through animal experiments and plasma metabolomics in conjunction with human network pharmacology and molecular docking analyses. We found that royal jelly acid could significantly decrease the serum lipid levels, ameliorate hepatic pathological injury, and reduce the level of oxidative stress in the experimental rats. A total of 41 key metabolites and 10 hub targets played key roles in the exertion of anti-hyperlipidemic effects, including tumor necrosis factor (TNF), insulin (INS) and epidermal growth factor receptor (EGFR). A total of 24 pathways, including tryptophan, citrate cycle, and arachidonic acid metabolisms, were identified as the key pathways involved in royal jelly acid-alleviated hyperlipidemia. The present findings provide new insights into the pathogenesis, diagnosis, and treatment targets of hyperlipidemia as well as contribute to the development and utilization of royal jelly acid related products.
研究领域:
药理学
·
代谢组学
·
分子生物学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Qianqian Zhang,Qi Guan,Shengping Han,Xianzai Yan,Jiaxin Hong,Luying Duan,Guoqiang Wu,Yanping Hong,Lili Yu,Chunrong Wang
In this study, a simple, rapid and sensitive tryptophan (Trp) detection method was proposed, which combined surface enhanced Raman spectroscopy (SERS) with diazo coupling reaction and silver dendritic substrate. Two derivatization reagents, 4-mercaptoaniline (PATP) and 2-fluoro-4-mercaptoaniline (2-F-PATP) were used for derivatization reaction with Trp, which can be controlled by experimental parameters such as derivatization reagent concentration and sodium nitrite concentration. The partial least squares quantitative (PLS) detection model is applied in determining the concentration of Trp. The determination coefficient (R 2 ) of PLS model was 0.9617 based on PATP and 0.9950 on 2-F-PATP. The LOD of the two derivatization methods were as low as 10 −11 mol/L. This combined technique has the characteristics of simple operation, high sensitivity and good selectivity, and shows great potential in the detection of Trp in biological media.
研究领域:
分析化学
·
生物化学
✓ 已验证
产品关联
DOI
高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Qi Guan,Pei Zeng,Qianqian Zhang,Lili Yu,Guoqiang Wu,Yanping Hong,Chunrong Wang
In this study, a simple, rapid and sensitive method combining surface-enhanced Raman spectroscopy and Schiff base reaction was developed for the detection of tryptophan. This method does not require product separation to obtain a significant Raman signal of the derivatized product, and the derivatization reaction can be controlled by experimental parameters such as reaction temperature, time, concentration of derivatization reagent and concentration of sodium nitrite. The characteristic peak of the derivative of tryptophan (1620 cm −1 ) was selected for quantitative analysis, and the intensity of the characteristic Raman spectrum peak showed a linear relationship with the concentration of tryptophan (10 −8 –10 −4 mol/L) in the range of with a correlation coefficient R 2 of 0.9922. This assay combines surface-enhanced Raman spectroscopy and Schiff base reaction, which is characterized by high sensitivity and easy operation, and has good application prospects in the detection of tryptophan in food.
研究领域:
分析化学
·
食品科学
·
生物化学
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）