SCIENCE OF THE TOTAL ENVIRONMENT
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2020
·
IF 6.551
Min Xia,Xuedong Wang,Jiaqi Xu,Qiuhui Qian,Ming Gao,Huili Wang
The organophosphate flame retardant, tris (1-chloro-2-propyl) phosphate (TCPP), is ubiquitous in environmental matrices; however, there is a paucity of information concerning its systemic toxicity. Herein, we investigated the effects of TCPP exposure on zebrafish neurodevelopment and swimming behavior to elucidate the underlying molecular mechanisms of neurotoxicity. Under TCPP gradient concentration exposure, the hatching rates were declined by up to 33.3% in 72 hpf, and the malformation rates increased from 15% to 50%. Meanwhile, TCPP led to abnormal behaviors including decreased locomotive activity in the dark and slow/insensitive responses to sound and light stimulation of larvae. TCPP caused excessive apoptosis and ROS accumulation in early embryonic development, with hair cell defects and structural deformity of neuromast. Abnormal expression of neurodevelopment ( pax6a , nova1 , sox11b , syn2a , foxo3a and robo2 ) and apoptosis-related genes ( baxa , bcl2a and casp8 ) revealed molecular mechanisms regarding abnormal behavioral and phenotypic symptoms. Chronic TCPP exposure led to anxiety-like behavior and excessive panic, lower capacity for discrimination and risk avoidance, and conditioned place preference in adults. Social interaction tests demonstrated that long-term TCPP stress resulted in unsociable, eccentric, lonely and silent behaviors in adults. Zebrafish memory and cognitive function were severely reduced as concluded from T-maze tests. Potential mechanisms triggering behavioral abnormality were attributed to histopathological injury of diencephalon, abnormal changes in nerve-related genes at transcription and expression levels, and inhibited activity of AChE by TCPP stress. These findings provide an important reference for risk assessment and early warning to TCPP exposure, and offer insights for prevention/mitigation of pollutant-induced nervous system diseases.
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EUROPEAN JOURNAL OF INORGANIC CHEMISTRY
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2020
·Vol. 2020
·期 36
·3466-3473
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IF 2.529
Yongcen Lin,Xue Dong,Lang Zhao
Graphical In this paper, a highly efficient nano-catalyst was synthesized using thiosalicylic acid modified ZIF-(1:2.5) as precursor, which was used to catalyze the reduction of 4-nitrophenol, methyl orange, methylene blue, and congo red. The catalyst shows excellent performance. Mesoporous catalyst S-ZIF-(1:2.5)@Ni x S y was prepared using ZIF-(1:2.5) as a precursor. These composite materials were characterized by using scanning microscope (SEM), transmission electron microscopy(TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and N 2 adsorption-desorption techniques, and studied as catalysts for the reduction of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP), congo red (CR), methyl orange (MO), and methylene blue (MB). The BET specific surface area and pore volume of the catalyst were 167.49 m 2 /g and 0.32 m 3 /g respectively. The doping of S improves the catalytic performance of the catalyst. S-ZIF-(1:2.5)@Ni x S y showed an excellent catalytic rate ( k app = 22.3 × 10 –3 /s) in the experiment of reductive degradation of 4-NP. In addition, S-ZIF-(1:2.5)@Ni x S y also showed good catalytic activity for the degradation of CR, MB, and MO, completing the catalytic reaction within 60, 60, and 90 s, respectively. The k app values for CR, MB, and MO are 29.0 × 10 –3 /s, 44.0.0 × 10 –3 /s, and 29.2 × 10 –3 /s, respectively.
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ENVIRONMENTAL RESEARCH
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2020
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IF 5.715
Zhen-Ni Yang,Ya-Nan Hou,Bo Zhang,Hao-Yi Cheng,Yang-Chun Yong,Wen-Zong Liu,Jing-Long Han,Shuang-Jiang Liu,Ai-Jie Wang
Biologically synthesized palladium nanoparticles (bio-Pd) have attracted considerable interest as promising green catalysts for environmental remediation. However, the mechanisms by which microorganisms produce bio-Pd remain unclear. In the present study, we investigated the roles of Shewanella oneidensis MR-1 and its NADH dehydrogenases and hydrogenases (HydA and HyaB) in bio-Pd production using formate as the electron donor. The roles of NADH dehydrogenases and hydrogenases were studied by inhibiting NADH dehydrogenases and using hydrogenase mutants (Δ hydA , Δ hyaB , and Δ hydA Δ hyaB ), respectively. The results showed ~97% reduction of palladium by S. oneidensis MR-1 after 24 h using 250 μM palladium and 500 μM formate. Electron microscopy images showed the presence of bio-Pd on both the outer and cytoplasmic membranes of S. oneidensis MR-1. However, the inhibition of NADH dehydrogenases in S. oneidensis MR-1 resulted in only ~61% reduction of palladium after 24 h, and bio-Pd were not found on the outer membrane. The mutants lacking one or two hydrogenases removed 91–96% of palladium ions after 24 h and showed more cytoplasmic bio-Pd but less periplasmic bio-Pd. To the best of our knowledge, this is the first study to demonstrate the role of NADH dehydrogenases of S. oneidensis MR-1 in the formation of bio-Pd on the outer membrane. It also demonstrates that the hydrogenases (especially HyaB) of S. oneidensis MR-1 contribute to the formation of bio-Pd in the periplasmic space. This study provides mechanistic insights into the production of biogenic metal nanoparticles towards their possible use in industrial and environmental applications.
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INTEGRATED FERROELECTRICS
·
2021
·
IF 0.836
Chao Ge,Xiaofeng Li,Zhou Zhao,Zhifeng Yan,Dandan Lian,Jianjun Lu
Silicalite-1 nanosheets catalyst with a hierarchical architecture was hydrothermally synthesized using [C18H37-N+(CH3)2-C6H12-N+(CH3)2-C6H13](Br)−2 as template. It has been studied systematically by investigating the influence of different synthesis parameters and crystallization kinetics under tumbling conditions. Highly crystalline silicalite-1 nanosheets with large external surface was obtained by regulating the crystallization time and the amount of water, template and the alkalinity. It was examined as catalyst for vapor-phase Beckmann rearrangement of cyclohexanone oxime to caprolactam and exhibited an excellent catalytic performance and long catalytic lifetime. This can be attributed to nest silanol groups located on the large external surface of the nanosheets. Furthermore, the high mesoporosity of nanosheets shorten the diffusion path length and reduce coking deposition, which remarkably improve catalyst stability.
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TALANTA
·
2020
·
IF 5.339
Xiaofei Li,Yawen Zhang,Lulu Hao,Yanju Liu,Xia Wang,Huaixia Yang,Jinming Kong
Cytokeratin fragment antigen 21-1 (CYFRA 21-1) DNA is perceived as sensitive tumor marker for the diagnosis of non-small cell lung cancer and other tumor. Herein, linear chain poly(ε-caprolactone) (PCL) synthesized by ring-opening polymerization is applied to ultrasensitive label-free electrochemical impedance detection system for CYFRA 21-1 DNA. First, thiolated peptide nucleic acid (PNA) is self-assembled into the Au electrode surface through the formation of Au–S bonds, allowing the PNA to act as biomolecular probe and form PNA/DNA heteroduplex with the target DNA via specific hybridization. Then, PCL is conjugated to the immobilized DNA on the electrode via “carboxylate–Zr 4+ –phosphate” bridges. Finally, the electrochemical response of modified PNA/DNA/Zr 4+ /PCL electrode is determined by electrochemical impedance method to quantify of CYFRA 21-1 DNA. Under optimal conditions, this method exhibits highly sensitivity with a broad linear range (0.1 fM – 1 nM) ( R 2 = 0.995) and the limit of detection (LOD) is as low as 10.73 aM, which is equivalent to just 64 molecules in a 10 μL sample. What's more, the high selectivity , good anti-interference, label-free operation, and real-time monitoring in complex samples of the proposed strategy demonstrate its broad application for the early diagnosis and clinical monitoring.
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Materials Science & Engineering C-Materials for Biological Applications
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2020
·
IF 5.88
Yufei Tang,Lei Chen,Zihao Duan,Kang Zhao,Zixiang Wu
Barium titanate (BaTiO 3 ) has been used as a bone implant material because of its piezoelectric properties and the ability to promote cell growth when combined with hydroxyapatite. However, the brittleness of BaTiO 3 inhibits its use as a bone replacement material at load-bearing sites, and the reduction of BaTiO 3 content in the composite reduces its piezoelectric effect on bone growth. In this study, we explored a preparation method, which included directional freeze casting and self-solidification of bone cement, to obtain 1-3-type BaTiO 3 /PMMA bio-piezoelectric composites with a lamellar structure. The lamellar BaTiO 3 layer through the composite from the bottom to the top significantly improved the piezoelectric properties of the composite. In addition, the dendritic ceramic bridges on the BaTiO 3 pore walls can improve the compressive strength and elastic modulus of BaTiO 3 /PMMA bio-piezoelectric composites with a lamellar structure. More importantly, it was found that polarized lamellar BaTiO 3 could induce osteoblasts to grow in the direction of the BaTiO 3 layers. When the width of the BaTiO 3 layer was in the range of 8–21 μm, osteoblasts along the BaTiO 3 layer showed well growth, which can be of great value for the production of biomimetic bone units.
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DRUG METABOLISM AND DISPOSITION
·
2020
·Vol. 48
·期 10
·1074-1083
·
IF 3.231
Yingchun Chen,Cui Li,Yaodong Yi,Weijuan Du,Huidi Jiang,Su Zeng,Hui Zhou
Dehydrocorydaline (DHC), one of the main active components of Corydalis yanhusuo, is an important remedy for the treatment of coronary heart disease. Our previous study revealed a higher unbound concentration of DHC in the heart than plasma of mice after oral administration of C. yanhusuo extract or DHC, but the underlying uptake mechanism remains unelucidated. In our investigations, we studied the transport mechanism of DHC in transgenic cells, primary neonatal rat cardiomyocytes, and animal experiments. Using quantitative real-time polymerase chain reaction and Western blotting, we found that uptake transporters expressed in the mouse heart include organic cation transporter 1/3 (OCT1/3) and carnitine/organic cation transporter 1/2 (OCTN1/2). The accumulation experiments in transfected cells showed that DHC was a substrate of OCT1 and OCT3, with Km of 11.29 ± 3.3 and 8.96 ± 3.7 μM, respectively, but not a substrate of OCTN1/2. Additionally, a higher efflux level (1.71-fold of MDCK-mock) of DHC was observed in MDCK-MDR1 cells than in MDCK-mock cells. Therefore, DHC is a weak substrate for MDR1. Studies using primary neonatal rat cardiomyocytes showed that OCT1/3 inhibitors (quinidine, decynium-22, and levo-tetrahydropalmatine) prevented the accumulation of DHC, whereas OCTN2 inhibitors (mildronate and l-carnitine) did not affect its accumulation. Moreover, the coadministration of OCT1/3 inhibitors (levo-tetrahydropalmatine, THP) decreased the concentration of DHC in the mouse heart. Based on these findings, DHC may be accumulated partly by OCT1/3 transporters and excreted by MDR1 in the heart. THP could alter the distribution of DHC in the mouse heart.
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BIOMEDICINE & PHARMACOTHERAPY
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2020
·
IF 4.545
Gaojun Wu,Zhengxian Wang,Peiren Shan,Shanjun Huang,Shuang Lin,Weijian Huang,Zhouqing Huang
Background Myocardial remodeling caused by angiotensin II (Ang II) is essential for the pathological process of heart failure. Netrin-1, which is an axonal guidance cue, has been shown to be involved in the inflammatory response, tumorigenesis, and angiogenesis in non-neuronal tissues. However, the role of Netrin-1 in cardiac remodeling has not been fully elucidated. Methods The rat cardiomyocyte cell line H9c2 and primary neonatal rat cardiomyocytes were treated with Ang II. Cells were transfected with siRNA to silence Netrin-1 expression. Real-time polymerase chain reaction and Western blot analysis were used to detect the markers for fibrosis, apoptosis, and hypertrophy in cardiomyocytes. An Annexin V-EGFP/PI cell apoptosis detection kit was used to measure the level of apoptosis caused by angiotensin II. Results We found that Netrin-1 expression was upregulated in the H9c2 cells and the neonatal rat cardiomyocytes stimulated by Ang II. The increased Netrin-1 expression was decreased by valsartan to block AT1R. Importantly, the application of Netrin-1 siRNA significantly alleviated the degrees of myocardial hypertrophy, fibrosis (reflected by Myhc, collagen I, and TGF-β) and apoptosis (reflected by the level of Caspase 3, Bax, and Bcl-2) induced by Ang II. In addition, the silencing of Netrin-1 substantially decreased the phosphorylation of PKCα, JNK, and P38. We treated H9c2 cells with LY317615, SP600125, and SB203580, inhibitors of PKCα, JNK, and P38, respectively, thereby resulting in a substantial decrease in hypertrophy, fibrosis, and apoptosis. Conclusions Ang II produces cardiac hypertrophy, fibrosis, and apoptosis through the upregulation of Netrin-1 and the activation of the AT1R/PKCα/MAPK (JNK, P38) pathway. Suppression of Netrin-1 can relieve Ang II-induced cardiac remodeling via inhibition of the PKCα/MAPK (JNK and P38) signaling pathway. Thus, Netrin-1 may be a novel therapeutic target for Ang II-mediated cardiac remodeling.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2020
·
IF 5.774
Wenbo Chen,Zhenguo Zhang,Lei Hou,Cancan Yang,Hongcheng Shen,Kai Yang,Zhe Wang
This work mainly studies the CO 2 separation performance of a new type mixed-matrix membranes (MMMs) based on MOF-801 as the microporous filler and PIM-1 as the polymer matrix. MMMs was successfully formed via simple casting/solvent evaporation approach at room temperature. The samples were systematically characterized by SEM, XRD, TGA and FTIR, the permeability-selectivity of MMMs to CO 2 , O 2 and N 2 was studied by gas permeability test. The experimental results show that the uniform dispersion of the CO 2 -philic MOF-801 filler provides a channel for the rapid and selective transport of CO 2 , so the MOF-801/PIM-1 MMMs revealed much higher CO 2 permeability and CO 2 /N 2 ideal selectivity (CO 2 = 9686 Barrer, CO 2 /N 2 = 27, all selectivity in the paper is ideal) than pure polymer membrane. After 90-days aging test, the CO 2 permeability of MMMs remains above 70%, which reflects its good anti-aging performance. After 120 h continuous gas permeability test, it also shows good long-term working stability. The experimental results show that the good gas separation performance of MMMs exceeds the Robeson upper bound (2008), showing good application prospect for practical greenhouse gas CO 2 separation.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2020
·
IF 3.573
Hang Li,Yi Zhai,Xubin Zhang,Guojun Lv,Yu Shen,Xuqing Wang,Tao Jiang,Yuzhou Wu
Hierarchical iron-containing TS-1 zeolites (FTS) were successfully synthesized via post-treatment desilication over microporous Fe-TS-1, which were prepared through a one-step hydrothermal synthesis. The prepared samples were characterized by X-ray diffraction, N2 adsorption–desorption, field-emission scanning electron microscopy, transmission electron microscopy, Fourier transform infrared spectroscopy, ultraviolet visible spectroscopy, and X-ray fluorescence spectrometry. The experimental results illustrated that the MFI structure of all the FTS samples remained unchanged after NaOH desilication and acid treatment. The newly created mesopores can be controlled by changing the amount of framework Fe, as the extraction of Si could be affected because of the inhibitory effect of iron species during the desilication process. The catalytic performance of the prepared samples in phenol hydroxylation with H2O2 was investigated. Among all the samples, FTS-50-D showed a highest phenol conversion of 45.2% and a dihydroxybenzene selectivity of 95.2%. Besides, FTS-50-D also showed good reusability for five recycling tests.
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Catalysts
·
2020
·Vol. 10
·期 4
·377
·
IF 3.52
Wei Yu,Wenfeng Han,Yongnan Liu,Jiaqin Lu,Hong Yang,Bing Liu,Haodong Tang,Aimin Chen,Ying Li
BaClxFyas well as BaF2and BaClF catalysts were prepared by solid-state reaction at room temperature with Ba(OH)2as the precursor and NH4F/NH4Cl as the F and Cl sources. The catalysts were applied for the dehydrochlorination of 1-chloro-1,1-difluoroethane to vinylidene fluoride at 350 °C. The industrial manufacture of vinylidene fluoride (VDF) is carried out at 600–700 °C, whereas the BaClxFycatalysts provided a promising pathway to produce VDF at much lower temperatures. Unfortunately, the selectivity of VDF over BaF2decreased from 94% to 84% along with the deactivation of the BaF2catalyst monotonically. In the presence of small amounts of Cl in BaF2, stabilized selectivity was achieved. Over BaCl0.05F0.95, BaCl0.1F0.9and BaCl0.25F0.75, no decrease in VDF selectivity was observed. Clearly, the presence of small amounts Cl during solid-state preparation inhibited the growth of BaF2crystalline significantly. Far smaller particles were achieved. The particle size, or more precisely, the crystal size of the barium catalyst played a major role in the catalytic performance. In addition to the crystal growth, the presence of small amounts of Cl during catalyst preparation changed the chemical state of Ba, and therefore the adsorption and activation of the C–Cl bond for HCFC-142b were altered.Keywords:barium chlorofluoride;catalytic pyrolysis;barium fluoride;dehydrochlorination;1-chloro-1,1-difluoroethane;vinylidene fluoride
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Catalysts
·
2020
·Vol. 10
·期 3
·355
·
IF 3.52
Wucan Liu,Yongnan Liu,Kabozya M. Mardochee,Zhikun Wang,Shucheng Wang,Wei Yu,Jianjun Zhang,Wenfeng Han
SrF2promotes the dehydrochlorination (DeHCl) of 1,1-difluoro-1-chloroethane, which is the key process for the manufacture of VDF (vinylidene fluoride), one of the most typical fluorinated monomers. However, the selectivity is low as dehydrofluorination (DeHF) to VCF (vinylidene chlorofluoride) competes with the formation of VDF. In this study, SrF2@C (SrF2embedded in carbon) and SrF2@NC (N-doped carbon) catalysts were fabricated following calcination in N2with SrC2O4, PVDF (poly vinylidene fluoride) and urea as the precursors. The catalysts were characterized by XRD, SEM, TEM, and XPS. The results show that both the calcination temperature and N-doping play an important role in the conversion of HCFC-142b and the selectivity to VDF and VCF. Calcination at elevated temperatures enhances the Sr-C interaction. For SrF2@C, improved interaction facilitates withdrawing electrons from Sr by the carbon support. By contrast, the strong interaction of Sr with N-doped carbon supply electrons from N species to Sr. The electron deficiency of Sr is favorable for the adsorption of F with higher electronegativity and consequently, DeHF reaction forming VCF. The supply of electrons to Sr by the support improves the formation of VDF (DeHCl). The present work provides a potential strategy for the improvement of selectivity to the target product.Keywords:dehydrochlorination;dehydrofluorination;metal–support interaction;strontium fluoride;vinyl difluoride
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