ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Juan Li,Ming-Chun Lai,Ya-Meng Zhong,Ya-Ling Chen,Na Wu,Wei Chen,Hua-Ping Peng
Monitoring D-amino acids concentrations has essential implications for gastric cancer diagnosis and treatment, especially in the non-invasive detection of gastric cancer. However, it remains a challenge to establish a high-performance detection method for D-amino acids. Here, a highly sensitive and selective d -alanine (D-Ala) ECL assay strategy via a turn-off-on split-type electrochemiluminescence (ECL) platform has been proposed. We found that hydrogen peroxide (H 2 O 2 ) could be used as an efficient etching agent to turn on the MnO 2 /gold nanocluster (AuNC)-based ECL nanoswitch platform. Based on abovementioned characteristics, we extended it to the D-amino acids assay since the enzymatic reaction between D-Ala and D-amino acid oxidase (DAAO) generates H 2 O 2 . Based on the abovementioned characteristics, this ECL sensing platform achieved a preferable linear-dependent curve in the detection range of 1.0 × 10 − 10 ∼1.0 × 10 − 3 mol L −1 , and realized the detection of D-Ala as low as 2.2 × 10 − 11 mol L −1 (S/N = 3). Furthermore, the proposed ECL biosensor showed excellent selectivity, stability, and reproducibility. Together with its powerful performance, this strategy could test D-Ala in saliva samples, which suggested that this ECL assay platform shows great prospect in disease diagnosis. We ascribe the high sensitivity and good anti-interference capability of the sensor to the combination of specific enzyme catalysis reaction, high-efficiency split-type AuNC probe-based ECL technique and the highly etching efficiency of H 2 O 2 to MnO 2 nanomaterials on electrode surface. In our perception, this H 2 O 2 mediated split-type ECL sensing platform provides a viable tool in ECL based bioananlysis. Therefore, our proposed approach not only provides a strategy for developing a high-performance platform for D-Ala detection, but also establishes a framework for the detailed design and development of ECL platform for other biological assays.
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ACS Applied Materials & Interfaces
·
2024
·
IF 8.3
Yuelong Xu,He Wang,Changrui Xing,Jianhao Zhang,Wenjing Yan
Bacterial infection has always posed a severe threat to public health. Gold nanoparticles (Au NPs) exhibit exceptional biocompatibility and hold immense potential in biomedical applications. However, their antibacterial effectiveness is currently unsatisfactory. Herein, a chiral antibacterial agent with high stability was prepared by the modification of Au NPs with d-cysteine with the assistance of polyethylene glycol (PEG). The as-synthesized d-cysteine/PEG-Au NPs (D/P-Au NPs) exhibited a stronger (99.5–99.9%) and more stable (at least 14 days) antibacterial performance against Gram-negative (Escherichia coli and Listeria monocytogenes) and Gram-positive (Salmonella enteritidis and Staphylococcus aureus) bacteria, compared with other groups. The analysis of the antibacterial mechanism revealed that the D/P-Au NPs mainly affected the assembly of ribosomes, the biosynthesis of amino acids and proteins, as well as the DNA replication and mismatch repair, ultimately leading to bacterial death, which is significantly different from the mechanism of reactive oxygen species-activated metallic antibacterial NPs. In particular, the D/P-Au NPs were shown to effectively accelerate the healing of S. aureus-infected wounds in mice to a rate comparable to or slightly higher than that of vancomycin. This work provides a novel approach to effectively design chiral antibacterial agents for bacterial infection treatment.
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APPLIED THERMAL ENGINEERING
·
2024
·
IF 6.1
Zhao-ran Li,Gao-ning Liang,Yu-dong Ding,Qiang Liao,Xun Zhu,Min Cheng
As an important intermediary between the green energy and human society, the lithium-ion battery has promising prospects in the new energy vehicles, energy storage, and green development fields. However, lithium-ion batteries can generate a large amount of heat during operation. In addition, excess temperature or big temperature difference of the surface of the lithium-ion battery can reduce its performance, which also results in reducing its operational life and even triggers incidents such as fires and explosions. Therefore, studying efficient thermal management methods for lithium-ion batteries is crucial for increasing their application range. This paper proposes a novel thermal management method by combining 3-D finned tubes with PCM. The thermal management system maintains a battery operating temperature less than 45 °C and a maximum temperature difference less than 2.4 °C. The influence of different fin structure parameters on the melting dynamics of PCM and the heat transfer performance is then discussed. The obtained results show that under each solid–liquid ratio of PCM, the heat transfer performance increases with the increases of the fin height, while it initially decreases and then increases with the increase of the width and axial spacing. Moreover, the heat transfer performance of the 3-D finned tube with different PCM liquid–solid ratios is investigated. The obtained results demonstrate that for constant fin parameters, the heat transfer reaches its highest performance for a PCM liquid–solid ratio greater than 2:2. The findings of this study lay a foundation for the design of efficient thermal management systems incorporating PCM applications.
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Molecular Medicine Reports
·
2019
·Vol. 20
·期 1
·332-340
·
IF 1.851
Lina Liu,Jing Yan,Fei Ge,Xiangtao Xu,Jun Lu,Huilian Shi,Shuihong Li,Yue Zhao,Changzheng Zhang
Saikosaponin‑D (SSD), which is the main bioactive component in the traditional Chinese medicine Chai Hu (Bupleurum falcatum L), possesses estrogen‑like properties and is widely used in treating estrogen‑related neurological disorders. The current study aimed to investigate the protective effects of SSD on the fear memory deficit in ovariectomized (OVX) rats and the potential underlying mechanism. SSD treatment significantly prolonged freezing time in OVX rats in a manner similar to that of estradiol (E2), whereas this effect was markedly suppressed by co‑administration of ICI182780, a non‑selective estrogen receptor (ER) inhibitor. The expression of ERα in the hippocampus of OVX rats was significantly elevated by SSD; however, Erβ expression and E2 synthesis were not markedly affected by SSD treatment. Collectively, this study demonstrated that SSD‑mediated fear memory improvement in OVX rats may be attributed not to E2 levels or ERβ activity, but to ERα activation in the hippocampus.
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CELLULAR AND MOLECULAR NEUROBIOLOGY
·
2016
·Vol. 37
·期 5
·869-877
·
IF 2.328
Shen Hui,Wang Jie,Jiang Dan,Xu Pei,Zhu Xiaolu,Zhang Yuanyuan,Yu Xing,Won Moo-Ho,Su Pei Qing,Yan Bing Chun
Some anticonvulsant drugs are associated with cognitive ability in patients; Topiramate (TPM) is well known as an effective anticonvulsant agent applied in clinical settings. However, the effect of TPM on the cognitive function is rarely studied. In this study, we aimed to observe the effects of TPM on cell proliferation and neuronal differentiation in the dentate gyrus (DG) of the d -galactose-induced aging mice by Ki-67 and doublecortin (DCX) immunohistochemistry. The study is divided into four groups including control, d -galactose-treated group, 25 and 50 mg/kg TPM-treated plus d -galactose-treated groups. We found, 50 mg/kg (not 25 mg/kg) TPM treatment significantly increased the numbers of Ki-67 + cells and DCX immunoreactivity, and improved neuroblast injury induced by d -galactose treatment. In addition, we also found that decreased immunoreactivities and protein levels of antioxidants including superoxide dismutase and catalase induced by d -galactose treatment were significantly recovered by 50 mg/kg TPM treatment in the mice hippocampal DG ( P < 0.05). In conclusion, our present results indicate that TPM can ameliorate neuroblast damage and promote cell proliferation and neuroblast differentiation in the hippocampal DG via increasing SODs and catalase levels in the d -galactose mice.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Kai Yang,Yueshan Huang,Liyi Yao,Bin Huang,Weimin Zeng,Jin Huang
D-amino acid oxidase (DAAO) has long been recognized as a promising catalyst for chiral amino acid biosynthesis owing to its pronounced stereospecificity. However, its limited thermostability restricts industrial utility and poses challenges for protein engineering. Herein, we engineered a DAAO variant, M5 (S42D/T84D/H138N/A193R/E195Y/C234L), via a ProteinMPNN-guided multiple codon saturation mutagenesis approach. This variant exhibited superior catalytic efficiency (27.3 mM −1 s −1 , 29-fold relative to the starting enzyme) toward the non-natural compound D-glufosinate (D-PPT) and enhanced thermostability (519 min half-life at 50 °C, 208-fold relative to the starting enzyme). The evolution also expanded the enzyme's substrate scope toward several D-amino acids with different properties. The variant was proven able to completely transform 500 mM D-PPT in 7 h at a 100 mL scale with excellent stereospecificity. Molecular dynamics (MD) simulations gave the insight that the enhanced performance stems from altered surface charges and intramolecular/intermolecular networks. This strategy thus holds potential for engineering other enzymes involved in the biosynthesis of valuable products while reducing the screening costs during directed evolution.
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CONSTRUCTION AND BUILDING MATERIALS
·
2025
·
IF 8
Wen Yang,Yunbo Tong,Yong Yang,Qianping Ran,Lei Dong
The application of corrosion inhibitors is a widely adopted strategy for protecting reinforcing steel against electrochemical degradation. However, conventional direct-addition approaches often lead to premature inhibitor depletion and lack controlled-release functionality. In this study, a novel sustained-release capsule-type inhibitor was developed to combat chloride-induced steel corrosion. Ethyl cellulose with high alkali resistance, controllable release and good mechanical properties was used as the shell material and D -sodium gluconate as the core, using a phase separation method and the preparation of the capsule was verified by TG, FT-IR and SEM. The experiment showed that the resulting capsules displayed a "pea-pod" shape (about 0.5–1.0 μm in length and 0.2 μm in diameter) with a compact core/shell structure and when the core/shell ratio was 1:5, the maximum encapsulation efficiency of the capsules was 73.3 %. When dispersed in simulated concrete pore solution, the capsules enabled sustained release of D -sodium gluconate over multiple days. EIS and LPR were used for electrochemical test. And the electrochemical analysis confirmed that the capsule-based inhibitor effectively suppressed corrosion and improved corrosion resistance. Specifically, a dosage of 0.05 % resulted in an inhibition efficiency of 90.7 ± 1.2 % after 7 days. The core-to-shell ratio was identified as a critical factor governing both the release kinetics and the overall protective performance, with the 1:5 formulation demonstrating optimal corrosion inhibition. This study offers a promising strategy for the design and application of intelligent corrosion inhibitors in reinforced concrete structures.
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JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Jiaqi Hu,Mingyue Wang,Min Wang,Dandan Wang,Yuqiang Xiao,Yue Lan,Lingling Li,Meiling Yang,Minmin Wang,Leijiao Peng,Famin Ke,Xiurong Guo,Die Gao
Considering the efficient utilization of resources and the simultaneous detection of multiple analytes, achieving selective detection of diverse analytes on one fluorescent covalent organic framework (COF) is of great significance. Herein, a carboxyl-carbazole-rich fluorescent COF (COF-DABT) was strategically synthesized using 3,6-diamino carbazole, capable of forming halogen bonds with analytes, and 2,5-bis(4′-formylphenyl) terephthalic acid, rich in carboxyl groups, which interacts with various analytes and adjusts fluorescence performance via its electron-withdrawing properties. The introduction of carbazole imparts fluorescence to COF-DABT through the intramolecular charge transfer (ICT) effect, while enabling selective fluorescence quenching. Specifically, the ICT of carbazole can be disrupted via linear halogen bond interactions with TBBPS, resulting in selectively detection of TBBPS in a fluorescence-off mode. Furthermore, efficient enrichment of TBBPS is facilitated by halogen bonds with carbazole and hydrogen bonds with carboxyl groups on COF-DABT. Selective fluorescence quenching of COF-DABT by Fe 3 + is achieved through chelating effect around carboxyl group, as well as cation-π interaction with carbazole benzene ring. This enables high selectivity detection of Fe 3+ via a fluorescence-off mode. Additionally, Fe 3+ can be reduced by ascorbic acid through REDOX reaction, mitigating interference with TBBPS detection and facilitating fluorescence-off-on mode detection of AA. Besides, rich carboxyl groups on COF-DABT exert a negative regulatory effect on the carbazole ICT mechanism. To counter this, D/L-Arg, with a size complementary to the adjacent carboxyl groups on COF-DABT, is introduced. Hydrogen bond interactions between D/L-Arg and carboxyl groups reduce the negative regulatory effect, allowing fluorescence enhancement for highly selective detection of D/L-Arg. Through rational design and functional optimization of fluorescent COF structures, multi-mode detection methods enable simultaneous detection of different analytes, achieving the goal of "one stone, several birds."
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JOURNAL OF SOLID STATE ELECTROCHEMISTRY
·
2021
·Vol. 25
·期 6
·1847-1859
·
IF 2.647
Li Chunxia,Guo Xin,Du Hongmei,Zhao Jinsheng,Liu Lixia,Yuan Qing,Fu Chonggang
Two novel donor-acceptor (D-A)-conjugated polymers, poly[(thiophene-2,5-yl)-((benzo[1,2-b:6,5-b′]dithiophene-4,5-dione)-2,7-yl)] (PTDT) and poly[((2,3-dihydrothieno[3,4-b] [ 1 , 2 ]dioxine)-5,7-diyl)-((benzo[1,2-b:6,5-b′]dithiophene-4,5-dione)-2,7-diyl)] (PTDO), have been synthesized with the same acceptor unit of benzo[1,2-b:6,5-b′]dithiophene-4,5-dione and different donor units of thiophene (Th) and 3,4-ethylenedioxothiphene (EDOT), respectively. And then, two composites (PTDT@C and PTDO@C) are made by coating the polymers on the surface of active carbon with an in situ method, which are used as the electrode materials of lithium-ion battery. The reversible capacities of PTDT@C and PTDO@C are 246.9 and 322.3 mAh g −1 at 0.1 A g −1 after 300 cycles running, respectively. By deducing the capability contribution of pure carbon, the theoretical capacities of the pure polymers are calculated to be 596 and 991 mAh g −1 , respectively, for PTDT and PTDO, which correspond to the lithium storage number of 6.7 and 13.3, respectively, for the least repeating unit of the polymers. With the help of theoretical calculations, the underlying mechanisms for the different electrochemical performances of the polymers are discussed and revealed. The structure-activity relationship discussed in this work may provide the design principle for the construction of the D-A-type polymers used as high-capacity electrode materials for organic rechargeable batteries.
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Small
·
2025
·
IF 12.1
Qing Sun,Xiangjun Zhou,Yanfeng Zhang,Chunlin Qin,Jiahui Wang,Yaman Cui,Hongyu Gong,Xuecheng Cao,Xiangjun Zheng
Designing efficient bifunctional electrocatalysts for the kinetically divergent hydrogen and oxygen evolution reactions (HER/OER) is challenging due to their conflicting interfacial requirements. Herein, precisely engineered RuS 2 /(FeNi)S 1.03 heterointerfaces are constructed via homogeneous chelation-coupled in situ sulfidation, enabling distinct interfacial mechanisms that independently enhance HER and OER. For HER, the RuS 2 component exclusively optimizes HER through static electronic modulation, where interfacial electron transfer at RuS 2 /(FeNi)S 1.03 interface downshifts the Ru d-band center to −1.99 eV and weakens hydrogen adsorption ( 0.51 eV). This yields superior HER activity with an overpotential of 270 mV@ at 50 mA cm −2 . Conversely, for OER, the (FeNi)S 1.03 phase undergoes dynamic reconstruction, transforming into an OER-catalytically active Fe-NiO oxide with stable RuS 2 (RuS 2 /Fe-NiO interfaces) that reduce intermediate energy barriers and delivers an overpotential of 300 mV at 50 mA cm −2 . This dual-interface synergy enables outstanding bifunctional performance with low cell voltages (1.68 V alkaline water; 1.76 V seawater) and industrial stability (>250 h at 1000 mA cm − 2 ), resolving the mechanistic conflict in bifunctional catalyst design.
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Food Science & Nutrition
·
2025
·Vol. 13
·期 11
·e71153
·
IF 3.8
Jinjia Liu,Yuning Zhang,Furong Lv,Chengming Wang,Jixiang Wang,Lijuan Li,Jinqiang Wu,Lina Lai
In this study, we aimed to investigate the hepatoprotective effects of plantagoside (PLA) against D-galactose (D-gal)-induced liver injury in aged mice. PLA treatment significantly alleviated the D-gal-induced body weight loss and the elevated liver index in mice, and effectively reduced oxidative stress, inflammatory response, and liver tissue damage in vivo. Transcriptomic analysis identified 513 differentially expressed genes (DEGs) regulated by PLA, and enrichment analysis revealed that these genes were primarily associated with the PI3K-Akt signaling pathway and purine metabolism. Metabolomic analysis revealed that PLA regulates purine metabolism by modulating key metabolites, such as hypoxanthine and cAMP. Integrated omics analysis suggested that PLA exerts protective effects by regulating purine metabolism, thereby enhancing antioxidant capacity and suppressing inflammation. In conclusion, PLA significantly alleviated D-gal-induced liver injury, reduced oxidative stress, and attenuated inflammation by regulating purine metabolism, thereby demonstrating its potential as an anti-aging and hepatoprotective drug and providing a new theoretical basis for the treatment of age-associated liver diseases.
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ChemSusChem
·
2025
·Vol. 18
·期 16
·e202500950
·
IF 6.6
Shuai Wang,Yihu Ke,Fei Jin,Youji Li,Zhiliang Jin
Noble metal nanoparticle co-catalysts offer high photocatalytic activity but are costly and scarce, driving the search for efficient, economical alternatives. We constructed a FeCo 2 O 4 /ZnCdS composite catalyst using electrostatic self-assembly. Optimizing the FeCo 2 O 4 loading to 10 wt% achieved a remarkable hydrogen production rate of 9080 μmol g −1 h −1 −3.49 times higher than pristine ZnCdS and exceeding precious metal co-catalysts like Au. Kelvin probe force microscopy, X-ray photoelectron spectroscopy, and density functional theory (DFT) calculations reveal an S-scheme heterojunction forms between FeCo 2 O 4 and ZnCdS. The resulting internal electric field efficiently drives directional charge migration and significantly suppresses electron-hole recombination. DFT further shows the interface electric field shifts the d-band center, optimizing reactant molecule adsorption. This avoids catalyst deactivation from strong adsorption while overcoming the energy barrier from weak adsorption, creating an ideal moderate-strength activated state. This work deepens understanding of S-scheme mechanisms and provides a new strategy for economical photocatalytic hydrogen production. Graphical The built-in electric field in FeCo 2 O 4 /ZnCdS heterojunctions regulates the d-band center to optimize hydrogen adsorption energy. An S-scheme charge transfer mechanism achieves a photocatalytic hydrogen evolution rate of 9080 μmol g − 1 h − 1 , surpassing noble-metal cocatalysts. Density functional theory and Kelvin probe force microscope studies confirm that interfacial electric field engineering enhances carrier separation and reaction kinetics, providing an efficient noble-metal-free strategy.
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