Pharmaceuticals
·
2026
·Vol. 19
·期 4
·640
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IF 5.7
Zeting Tan,Rongshun Liang,Adam Junka,Haoxuan Sun,Jie Jiang,Haojia Ma,Shisong Fang,Yanfang Sun
Background: Respiratory syncytial virus (RSV) is a leading global pathogen of acute lower respiratory tract infection, posing significant risks to infants, the elderly, and immunocompromised patients.Artemisia argyiLevl.et Vant Extract (AALE) and its active components have a variety of pharmacological effects, but their anti-RSV potential remains unclear. The aim of this study is to investigate the anti-RSV activity of AALE and parthenolide and its underlying mechanisms.Methods: Cell counting kit-8 (CCK-8) assay was used to determine the anti-RSV activities of AALE and parthenolide. Time-of-addition assay and phase of action analysis were used to explore the effect of drugs on the viral replication cycle. Quantitative polymerase chain reaction (qRCR), immunofluorescence (IF) and Western blot (WB) were used to investigate the effects of AALE and parthenolide on RSV-F gene and protein and on RIG-I/TLR-3 pathway related molecules in vitro. In vivo antiviral efficacy was verified by hematoxylin–eosin (HE) staining for lung histopathology, quantitative real-time PCR (qPCR) quantification of RSV-F, RIG-I, TLR-3, IRF3, IL-6, and IFN-β gene expression in lung tissues, and enzyme-linked immunosorbent assay (ELISA) for serum IL-6 and IFN-β levels.Results: AALE exhibited the strongest anti-RSV activity among the extracts (SI = 27.6), while parthenolide was the most potent monomeric compound (SI = 8.19). In vitro, both AALE and parthenolide were effective in the co-treatment and post-treatment models, reducing RSV-F gene and F protein levels in infected cells. Furthermore, they alleviated RSV infection by regulating RIG-I and TLR-3 pathway-related genes and proteins. In vivo, AALE and parthenolide suppressed lung index and RSV proliferation, attenuated lung injury, and down-regulated RIG-I, TLR-3, IRF3, IL-6, and IFN-β expression in the lungs of RSV-infected mice.Conclusions: AALE and its component parthenolide can inhibit the invasion and replication of RSV, making it a potential candidate for the treatment of RSV-related diseases.
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INORGANIC CHEMISTRY
·
2026
·
IF 4.6
Yingying Fang,Guangliang Liu,Ying Wang,Ligang Hu,Yong Liang,Yongguang Yin,Yong Cai,Guibin Jiang
Mercury (Hg) biogeochemical cycling involves redox transformations between Hg(0) and Hg(II), likely via a mercurous Hg(I) intermediate. Despite previously thought metastable, Hg(I) was found to exist stably in natural waters; yet, the redox behavior governing Hg(I) occurrence remains elusively studied. Here, we simulated an environmental Eh gradient (0.219–0.600 V, vs SHE) using noninterfering 1,4-benzoquinone and 1,4-hydroquinone as redox mediators, and demonstrate that Hg(I) redox transformations are strongly Eh-dependent. Hg(I) was stable at 0.302–0.339 V, contrasting significant oxidation (19–98%) at 0.369–0.600 V or reduction (81%) at 0.219–0.255 V. Speciation analysis and thermodynamic calculations confirm that varying proton level alters Hg(II) species abundance (HgHPO4, HgPO4–, and Hg(OH)2), driving this Eh-dependence. Specifically, HgHPO4/HgPO4– exhibits lower reduction potentials (to Hg(I), as Hg2(OH)2) than Hg(OH)2. Consequently, Hg(I) is subject to oxidation to HgHPO4/HgPO4– at 0.369–0.440 V (pH 6.0), while Hg(OH)2 preferentially undergoes stepwise reduction to Hg(I) and Hg(0) at 0.236–0.308 V (pH 7.5). Additionally, externally forced Hg(0) removal processes, e.g., purging used in conventional Hg(0) analysis, significantly affect Hg(I) transformations and enhance Hg reduction under reducing conditions, whereas natural degassing has negligible effects. This work provides valuable parameters for Hg redox thermodynamics and important implications for predicting Hg(I) occurrence in aquatic environments.
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ANALYTICAL LETTERS
·
2022
·
IF 2.267
Ruihuan Zhao,Nan Yin,Lixin Ma,Jie Zhang,Yangkai Luo,Zhimin Guo,Mengmei Fa,Dingding Yang,Dengchao Wang,Xin Yao
ATP is related to the occurrence of many diseases, its facile, sensitive and accurate determination in real samples is needed for disease screening and treatment. Although some methods realized the sensitive detection of ATP using the signal amplification of cyclic reactions or nanoparticles, complex experimental processes limit their applications. In this paper, we report a simple surface plasmon resonance (SPR) method based on a single stranded DNA probe. The probe has dual recognition sites of ATP and Ag+; namely the ATP aptamer located in the middle of the probe and five cytosine (C) at both ends of the probe. In the absence of ATP, the probe transforms into a rigid hairpin structure by forming C-Ag+-C structures and produces a large SPR signal. However, once the probe interacts with ATP, three-dimensional structures are formed, and then the Ag+-induced probe configuration change is smaller, resulting in an small SPR signal. The signal difference before and after the probe interacts with ATP is proportional to the analyte concentration. The probe configuration change is in the optimum area for SPR detection, ensuring the sensitive ATP determination. The signal difference has a linear response with the logarithm of ATP concentration from 0.05 to 500 nM with a detection limit of 15 pM. Moreover, the strategy was demonstrated to determine ATP in serum samples with satisfactory recoveries. The results show that this simple and sensitive method has potential applications for clinical ATP determination.
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Journal of Materials Chemistry B
·
2022
·Vol. 10
·期 32
·6107-6117
·
IF 7.571
Siying Xie,Cheng Qin,Furong Zhao,Zhaoyang Shang,Pei Wang,Muhammad Sohail,Xing Zhang,Bingzhi Li
CRISPR-driven biosensing is developing rapidly, but current studies mostly adopt dye-labeled ssDNA as the signal reporter, which is costly and unstable. Herein, we developed a label-free and low-background reporter for CRISPR/Cas12a signaling by integrating DNA-templated copper nanoclusters (DNA–CuNCs) and exonuclease I (EXO I). The template of the DNA–CuNCs was rationally designed as a ds-/ss-DNA hybrid, ensuring that after a quick and nonpersistent cut of Cas12a, a majority of the template can be digested by EXO I. Based on this novel reporter, a biosensor termed CRISPR-CNS (cost-effective, nimble, and sensitive copper nanocluster sensor integrating CRISPR) was developed. Due to the high signal-to-background ratio of our proposed reporter, CRISPR-CNS shows excellent performances for nucleic acid detection, yielding a detection limit of 20 copies for SARS-CoV-2 RNA. Considering its facile synthesis, robust fluorescence, effective cost, and good sensitivity, this combination shall serve as a highly potential output for CRISPR-based point-of-care testing.
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JOURNAL OF MOLECULAR LIQUIDS
·
2022
·
IF 6.633
Yuanhui Chong,Qiong Liu,Zeqi Wang,Liukai Zhu,Fengxin Guo,Yu Li,Tao Li,Baozeng Ren
Perphenazine has rather effective in the treatment of mental illness. In this work, the solid-liquid equilibrium data of perphenazine in eleven organic neat solvents were determined by laser dynamic method under the atmospheric pressure and temperature range of 278.15 K ∼ 318.15 K. The selected solvents were n-propanol, n-butyl alcohol, methyl acetate, ethyl acetate, n-propyl acetate, n-amyl acetate, N, N-dimethylformamide (DMF), N, N-dimethylacetamide (DMAC), acetone, 2-methoxyethanol, glycol ether, respectively. From the results, the solubility of perphenazine is all positively correlated with temperature. Separately, we have adopted the KAT-LSER model and molecular dynamics simulation to investigate the influence of solvent effects and solute-solvent interaction on solute solubility. Meanwhile, there were four activity coefficient models being used to correlate the solubility data and Average relative deviation of models are 4.13% (Margules model), 3.94% (NRTL model), 4.49% (NRTL-SAC model) and 3.06% (UNIQUAC model), respectively, all of which do not exceed 5%. It manifests that the four activity coefficient models can fit the experimental values well, in which the UNIQUAC model gives the best regression effect. Moreover, the apparent thermodynamic properties( △ sol G° , △ sol H° and △ sol S° ) of perphenazine in various solvents were calculated by Van’t Hoff equation. In accordance with the analysis and calculation of dissolution, the dissolution process is an endothermic entropy-driven process. This research can provide basic data for the crystallization process of perphenazine.
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JOURNAL OF ANALYTICAL AND APPLIED PYROLYSIS
·
2021
·
IF 5.541
Yongdi He,Jinou Song,Yuan Zhuang,Jiuzhong Yang,Zhongwei Meng,Qiang Xu
An experimental study of 1-nitropropane (1-NP) pyrolysis was performed in a flow reactor at low pressure (4.0 kPa) in the temperature range of 623–973 K. The pyrolysis species was detected with the tunable synchrotron vacuum ultraviolet (VUV) photoionization and molecular-beam mass spectrometry (MBMS). Species from m/z = 15–87 were identified from near-threshold photoionization efficiency (PIE) spectra, such as radicals of CH 3 , C 2 H 5 , C 3 H 5 and C 3 H 7 ; isomers of C 2 H 4 O (ethenol and acetaldehyde), C 3 H 6 O (acetone and propanal), C 4 H 10 (butane and isobutane), HNC (hydrogen isocyanide), HCN (hydrogen cyanide)), C 3 H 5 N (ethyl isocyanide and species with unidentified structure) and C 3 H 5 NO (methoxyacetonitrile and species with unidentified structure). Their mole fraction profiles were evaluated at different temperature. Based on the mole fractions of species measured at the minimum temperature of 1-NP pyrolysis, some new channels of 1-NP primary pyrolysis were speculated, such as the channels involving O-transfer from N to C-atoms in different positions, deoxygenation, dehydrogenation and OH-abstraction. Based on the mechanisms proposed in the literature, the species produced from secondary or subsequent reactions were also discussed. These results are valuable to a further quantum mechanical calculation for a better understanding of 1-NP chemistry.
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CHEMICAL ENGINEERING JOURNAL
·
2021
·
IF 13.273
Weibin Chen,Changshui Wang,Shibiao Su,Hai Wang,Dandan Cai
The quest for desirable two-dimensional (2D) materials and facile strategies to construct 2D layered composites with fascinating structures and compositions for high-performance electrocatalysis is still a major challenge. In the present research, novel nanocomposites of 2D zeolitic imidazolate framework-9 (ZIF-9) combined with Co-layered double hydroxide (Co LDH) were successfully synthetized through a controllable phase transition from ZIF-9(I). The 3D to 2D structural transformation of ZIF-9 was induced by deionized water, and the formation of 2D Co LDH was simultaneously realized during the one-step fabrication of ZIF-9(III)/Co LDH nanocomposites. Due to the high surface area, the presence of abundant metal sites, and the interactions between 2D ZIF-9(III) and 2D Co LDH, the as-optimized ZIF-9(III)/Co LDH nanocomposite exhibits great electrochemical activities for oxygen evolution reaction (OER) (η = 297 mV at 10 mA cm −2 ) in 1.0 M KOH solution. The electrochemical performance of the ZIF-9(III)/Co LDH nanocomposite was superior to those of the pure Co LDH, ZIF-9(III), and commercial RuO 2 . The present work demonstrates that the proposed strategy can effectively boost catalytic activities and also design new routes to synthesize advanced MOF nanohybrids for various applications.
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MOLECULAR PHARMACEUTICS
·
2020
·
IF 4.321
Dandan Miao,Yanyan Yu,Yong Chen,Yin Liu,Gaoxing Su
Stimuli-responsive DNA-based nanostructures have emerged as promising vehicles for intelligent drug delivery. In this study, i-motif DNA-conjugated gold nanostars (GNSs) were fabricated in a facile manner as stimuli-responsive drug delivery systems (denoted as A-GNS/DNA/DOX) for the treatment of cancer via combined chemo-photothermal therapy. The i-motif DNA is sensitive to the environmental pH and can switch from a single-stranded structure to a C-tetrad (i-motif) structure as the environmental pH decreases from neutral (∼7.4) to acidic (<6.0). The loaded drug can then be released along with the conformational changes. To enhance cellular uptake and improve cancer cell selectivity, the aptamer AS1411, which recognizes nucleolins, was employed as a targeting moiety. The A-GNS/DNA/DOX nanocomposites were found to be highly capable of photothermal conversion and exhibited photostability under near-infrared (NIR) irradiation, and the pH and NIR irradiation effectively triggered the drug-release behaviors. In addition, the A-GNS/DNA/DOX nanocomposites exhibited good biocompatibility. The targeting recognition enabled the A-GNS/DNA/DOX to exhibit higher cellular uptake and therapeutic efficiency than the GNS/DNA/DOX. Notably, under NIR irradiation, a synergistic effect between chemotherapy and photothermal therapy can be achieved with the proposed delivery system, which exhibits much higher therapeutic efficiency both in monolayer cancer cells and tumor spheroids as compared with a single therapeutic method. This study highlights the potential of GNS/DNA nanoassemblies for intelligent anticancer drug delivery and combined cancer therapy.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2019
·
IF 5.107
Sharafat Ali,Izaz Ali Shah,Haiou Huang
The need for high-performance and novel technologies for selective treatment of radionuclides in water has recently stimulated broad research. In this study, we examined the ability of carbon nanotube (CNT) membranes in selective removal of strontium (Sr 2+ ) and cesium (Cs + ) from water and wastewater. In single-metal systems that contained only Sr 2+ , Cs + or their competitive metal ions, the distribution coefficients ( K d ) of functionalized CNT membranes for divalent cations, i.e., Sr 2+ , Ca 2+ , and Mg 2+ , were measured as 4.41 mmol g −1 , 2.14 mmol g −1 , and 1.30 mmol g −1 , respectively, and those for monovalent cations, i.e., Cs + , K + and Na + , were 0.81 mmol g −1 , 0.79 mmol g −1 , and 0.81 mmol g −1 , respectively. When Cs + or Sr 2+ co-existed with a competing cation, both pristine and functionalized CNT membranes showed excellent selectivity to Sr 2+ over Mg 2+ and Ca 2+ while their selectivity to Cs + over K + and Na + diminished. Moreover, the CNT membranes remained their selectivity for Sr 2+ in a wastewater effluent despite the complex water composition. Overall, the selectivity sequence of the CNT membranes for divalent cations support the occurrence of inner-sphere complexation on CNT surfaces, while that for the monovalent cations was probably determined by electrostatic interactions. This study demonstrates that Ar/O 2 plasma treatment is a greener technology for the production of CNT functionalized membranes for selective removal of contaminants from water to achieve “fit-for-purpose” treatment.
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INORGANIC CHEMISTRY
·
2022
·
IF 5.436
Wenli Song,Bing Zhang,Wenli Zhou,Jilin Zhang,Liping Yu,Shixun Lian
All-inorganic perovskite quantum dots (PQDs, CsPbX3, X = Cl, Br, and I) show outstanding application prospects in the field of photoelectric devices. In recent years, the development of PQDs has greatly improved their stability to water, oxygen, and light. However, thermal quenching of PQDs greatly limits their practical application. Herein, we embed PQDs into ATiO3 (A = Ca, Ba, and Sr) of three different mesoporous spherical structures to explore the effect on thermal quenching of PQDs. Because of the unique mesoporous hollow microsphere structure and low thermal conductivity of SrTiO3, it can effectively block the heat transfer and improve the thermal quenching of PQDs. The photoluminescence (PL) intensity of CsPbBr3@SrTiO3 composites is 72.6% of the initial intensity after heating to 120 °C. Moreover, the PL intensity of CsPbBr3@SrTiO3 composites remains about 80% of the initial value even when stored in air for 20 days or irradiated by 365 nm UV light for 48 h. A neutral white light-emitting diode is assembled by a blue chip, CsPbBr3@SrTiO3 composites, and red phosphor of K2SiF6:Mn4+, which has a color temperature of 5389 K and a color gamut covered 133% of National Television Standards Committee (NTSC).
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ACS Applied Nano Materials
·
2022
·
IF 6.14
Sheng Zhang,Zhenyang Liu,Linlin Mao,Jian Wu,Di Zhang,Ruiyuan Liu,Li Qi
High quality visualization of live mammalian embryo is an important requirement for studying fetal development. Photoacoustic tomography (PAT) and optical coherence tomography (OCT) are two advanced imaging modalities that have been utilized for embryonic imaging separately. However, high contrast, multiscale, and deep tissue imaging of embryos in vivo remains challenging. Here, we demonstrate multimodal contrast-enhanced imaging of live embryos by gold nanostar-meditated near-infrared I (NIR-I) PAT and near-infrared II (NIR-II) OCT. We investigate intravenous and intravaginal administration of gold nanostars, and significant enhancement of signal, image contrast, and imaging depth are achieved for both PAT and OCT. Our findings may facilitate detailed studies of the morphological and functional changes during embryonic development.
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CHEMOSPHERE
·
2023
·
IF 8.8
Huiwang Dai,Jingyi Zhu,Fanxu Meng,Daohui Lin,Wenjun Zhou,Dingjiang Chen,Ming Zhang,Qingqing Wang
In this study, Cu hybridization coupling oxygen defect engineering was adopted to synthesis of CuNiFe layered double oxides (CuNiFe-LDOs) in peroxymonosulfate (PMS) activation for degradation of methyl 4-hydroxybenzoate. The morphology and crystal structure of CuNiFe-LDOs was characterized in detail, which exhibited regular layered-structure at a Cu:Ni doping ratio of 1:1 and annealing temperature of 400 °C, and presented the crystal of Cu x O@Fe 3 O 4 –NiO. Besides, the X-ray photoelectron spectroscopy (XPS) and electron paramagnetic resonance (EPR) results demonstrated that abundant oxygen vacancies (OVs) and low oxidation state Cu species were composed in CuNiFe-LDOs400. The Cu 1·5 Ni 1·5 Fe 1 -LDOs400/PMS system showed excellent catalytic performance toward the degradation of butyl 4-hydroxybenzoate (BuP), and resistant to the effect of pH value and background inorganic anions. Based on quenching experiments and EPR measurements, singlet oxygen ( 1 O 2 ) was identified as the dominant active species during the heterogeneous catalytic process, which was generated by the synergistic interaction between OVs-Cu(I) site and PMS . In this process, the electron-drawing property of OVs promoted the adsorption of PMS molecule on Cu(I) site, followed by the accumulation of electron and cleavage of O–O bond to generate intermediate oxygen radical species, which donated one electron to eventually generate singlet oxygen.
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