Polymer Chemistry
·
2024
·Vol. 15
·期 38
·3864-3870
·
IF 4.1
Siheng Zhang,Jianda Niu,Enci Hu,Liguo Xu,Zhixian Dong,Jinbao Xu,Caihong Lei
The synthesis of poly(L-lactide)-b-poly(amino acid) (PLA-b-PAA) block copolymers is of great interest in biomedicine due to their pronounced biodegradability and biocompatibility. However, the traditional method for preparing the functional block copolymers generally involves a tedious protection and deprotection process by covalent bonding, which may not only degrade the PLA segment during the process but also eliminate the precision in controlling the ROP of NCA. In light of this, in this study, PLA-b-PAAs were directly synthesized by the noncovalent protection between triethylborane (Et3B) and an amino alcohol initiator during the ring-opening polymerization (ROP) process of LA under mild reaction conditions, which produced amino-terminated PLA (PLA-NH2) avoiding the tedious process of the traditional method. Subsequently, the PLA-NH2 macroinitiator was employed to initiate the ROP of N-carboxyanhydride, thereby forming the well-defined PLA-b-PAA block copolymers. The noncovalent protection of hetero-initiators provides a promising platform for the design of PLA-b-PAA polymeric materials with tailored functionalities for biomedical applications.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2024
·
IF 3.3
Nawei Zhang,Yang Xu,Xiao Jia,Xiao Li,Jingnan Ren,Siyi Pan,Gang Fan,Jinchu Yang
BACKGROUND Citrus products often suffer from delayed bitterness, which is generated from the conversion of non-bitter precursors (limonoate A-ring lactone, LARL) to limonin under the catalysis of limonin D-ring lactone hydrolase (LDLH). In this study, LDLH was isolated and purified from sweet orange seeds, and a rapid and accurate high-performance liquid chromatography method to quantify LARL was developed and applied to analyze the activity and enzymatic properties of purified LDLH. RESULTS Purified LDLH (25.22 U mg −1 ) showed bands of 245 kDa and 17.5 kDa molecular weights in native polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate PAGE analysis respectively. After a 24 h incubation under strongly acidic (pH 3) or strongly alkaline (pH 9) conditions, LDLH still retained approximately 100% activity. Moreover, LDLH activity was not impaired by thermal treatment at 50 °C for 120 min. Enzyme inhibition assays showed that LDLH was inactivated only after ethylenediaminetetraacetic acid treatment, and other enzyme inhibitors showed no significant effect on its activity. In addition, the LDLH activity of calcium ion (Ca 2+ ) intervention was 108% of that in the blank group, and that of zinc ion (Zn 2+ ) intervention was 71%. CONCLUSION LDLH purified in this study was a multimer containing 17.5 kDa monomer with a wide pH tolerance range (pH 3–9) and excellent thermal stability. Moreover, LDLH might be a metallopeptidase, and its activity was stimulated by Ca 2+ and significantly inhibited by Zn 2+ . These findings improve our understanding of LDLH and provide some important implications for reducing the bitterness in citrus products in the future. © 2024 Society of Chemical Industry.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ENVIRONMENTAL MANAGEMENT
·
2024
·
IF 8
Yi Lu,Fangyuan Peng,Yingyang Wang,Zhaoguang Yang,Haipu Li
Ramie ( Boehmeria nivea L.) is a promising phytoremediation candidate due to its high tolerance and enrichment capacity for antimony (Sb). However, challenges arise as Sb accumulated mainly in roots, complicating soil extraction. Under severe Sb contamination, the growth of ramie may be inhibited. Strategies are needed to enhance Sb accumulation in ramie's aboveground parts and improve tolerance to Sb stress. Considering the beneficial effects of selenium (Se) on plant growth and enhancing resistance to abiotic stresses , this study aimed to investigate the potential use of Se in enhancing Sb uptake by ramie. We investigated the effects of Se (0.5, 1, 2, 5, or 10 μM) on ramie growth, Sb uptake and speciation, antioxidant responses, and ionomic profiling in ramie under 10 mg/L of SbIII or antimonate (SbV) stresses. Results revealed that the addition of 0.5 μM Se significantly increased shoot biomass by 75.73% under SbIII stress but showed minimal effects on shoot and root length in both SbIII and SbV treatments. Under SbIII stress, 2 μM Se significantly enhanced Sb concentrations by 48.42% in roots and 62.88% in leaves. In the case of SbV exposure, 10 μM Se increased Sb content in roots by 42.57%, and 1 μM Se led to a 91.74% increase in leaves. The speciation analysis suggested that Se promoted the oxidation of SbIII to less toxic SbV to mitigate Sb toxicity. Additionally, Se addition effectively minimized the excess reactive oxygen species produced by Sb exposure, with the lowest malondialdehyde (MDA) content at 0.5 μM Se under SbIII and 2 μM Se under SbV, by activating antioxidant enzymes including superoxide dismutase , catalase , peroxidase , and glutathione peroxidase . Ionomic analysis revealed that Se helped in maintaining the homeostasis of certain nutrient elements, including magnesium, potassium (K), calcium (Ca), iron (Fe), and copper (Cu) in the SbIII-treated roots and K and manganese (Mg) in the SbV-treated roots. The results suggest that low concentrations of Se can be employed to enhance the phytoremediation of Sb-contaminated soils using ramie.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
FOOD RESEARCH INTERNATIONAL
·
2024
·
IF 7
Chang Zhou,Yi-fan Hu,Yan Zhang,Cheng-hao Wang,Xue-jing Liao,Fa-feng Cheng,Yan-yan Jiang
Prunella vulgaris L. (P. vulgaris) has great application value and development prospects in improving sleep. In this study, we continued to evaluate the sleep-improvement function and mechanism of P. vulgaris from both chemical characterization and function based on sleep-improvement functional ingredients, rosmarinic acid and salviaflaside, screened out in the previous stage as the index components. The chemical constituents of P. vulgaris and its phenolic acid fraction were characterized by the UPLC-MS n technology. The quality of the sleep-improvement phenolic acid fraction of P. vulgaris was scientifically evaluated by fingerprints combined with quantitative analysis of rosmarinic acid and salviaflaside. The function of phenolic acid parts of P. vulgaris in improving sleep was verified by different insomnia models including the PCPA-induced insomnia model and surface platform sleep deprivation model. HE staining was used to observe the effect of P. vulgaris on the morphology of nerve cells in different brain regions. In vivo experiments and molecular docking explored the sedative-hypnotic effects of functional ingredients of P. vulgaris . All these results investigated the material basis and mechanism of P. vulgaris to improve sleep from multiple perspectives, which contribute to providing a basis for the development of functional food to improve sleep.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Plants-Basel
·
2025
·Vol. 14
·期 17
·2664
·
IF 4.1
Dan Wang,Yin Liu,Yao Zhang,Xiumei Huang,Jiaxuan Wang,Yi Wang,Yue Liu,Chao Yan,Bingsheng Lv,Yue Jia
Welsh onion ( Allium fistulosum L.), a globally significant vegetable, flavoring agent, and phytomedicine resource, has remained unavailable with established transient expression platforms for functional genomic investigations. To address this critical methodological limitation, we present systematically optimized protocols for both Agrobacterium -mediated hairy root transformation and protoplast transient expression systems, achieving significant advances in transformation efficiency for this species. Through systematic optimization of key parameters, including Agrobacterium rhizogenes ( A. rhizogenes ) strain selection (with Ar.Qual demonstrating superior performance), explant type efficacy, bacterial suspension optical density (OD 600 = 0.3), and acetosyringone induction concentration (100 μM), we established a highly efficient stem disc infection methodology, achieving 88.75% hairy root induction efficiency. Subsequent optimization of protoplast isolation protocols identified the optimal enzymatic digestion conditions: 6-h dark digestion of young leaves using 1.0% ( w / v ) Cellulase R-10, 0.7% ( w / v ) Macerozyme R-10, and 0.4 M mannitol, yielding 3.3 × 10 6 viable protoplasts g −1 FW with 90% viability. System functionality validation through PEG-mediated transient transformation demonstrated successful green fluorescent protein (GFP) reporter gene expression, confirmed by fluorescence microscopy. As the first documented transient expression platforms for Welsh onion, these protocols enable essential molecular investigations, including in planta promoter activity profiling, subcellular protein localization, and CRISPR-based genome-editing validation. This methodological breakthrough overcomes previous technical constraints in Welsh onion molecular biology, providing critical tools for accelerated gene functional characterization in this agriculturally important species.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOPROCESS AND BIOSYSTEMS ENGINEERING
·
2025
·
IF 3.6
Jin Li-Qun,Liu Meng-Dan,Guan Zi-Yu,Li Yi-Xin,Xue Ya-Ping,Liu Zhi-Qiang,Zheng Yu-Guo
DAAO is applied as a potential catalyst in the biosynthesis of L-PPT. However, its low solubility expression constrains its broader industrial application. Herein, a novel DAAO derived from Cladophialophora carrionii ( Cc DAAO) was identified, which demonstrated superior catalytic performance toward D-Ala (specific activity: 106.38 ± 1.21 U/mg, K m : 1.56 ± 0.06 mM), along with remarkable thermostability and broad substrate spectrum. Under optimal culture conditions, the soluble expression level of Cc DAAO was enhanced through a co-expression strategy with molecular chaperones, and the enzyme activity increased by 36.3% compared with the initial level. Subsequently, Cc DAAO was constructed as a fusion protein (CGD) with catalase from Geobacillus sp. CHB1 ( Gb CAT) and applied in a D-amino acid aminotransferase (DAAT)-mediated cascade system. In a 2 L reaction system, this cascade system achieved complete conversion (> 99%) of 1 M D,L-PPT within 8 h, exhibiting a yield of 11.26 g/L/h for PPO, which represents a significant improvement over existing reports. This study presents a promising practical approach for the industrial production of optically pure L-PPT.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 13
·2840
·
IF 4.6
Qiang Zhao,Weifei Zhang,Dan Song,Xirui Zhou,Xianjiang Li,Huan Yao,Wenjing Xing,Hongmei Li,Jian Ma,Peng Xiao
L-thyroxine (T4) is an important hormone for diagnosing and evaluating thyroid function disorders. As outlined in ISO17511, having a certified reference material (CRM) is crucial for ensuring that the results of clinical tests are traceable to the SI-unit. This study employed two principal methods to evaluate the purity of T4, mass balance (MB) and quantitative nuclear magnetic resonance (qNMR), both of which are SI-traceable (International System of Units) approaches. The MB method involved a detailed analysis of impurities, including water, structurally related compounds, and volatile and non-volatile substances. A variety of techniques were employed to characterize T4 and its impurities, including liquid-phase tandem high-resolution mass spectrometry, ultraviolet spectrophotometry, infrared spectroscopy, and both 1 H-NMR and 13 C-NMR. Additionally, impurities were quantified using Karl Fischer coulometric titration, ion chromatography, gas chromatography–mass spectrometry, and inductively coupled plasma–mass spectrometry. In qNMR, ethylparaben was used as the internal standard for direct value assignment. The results showed T4 purities of 94.92% and 94.88% for the MB and qNMR methods, respectively. The water content was determined to be 3.563% (n = 6), representing the highest impurity content. Ten structurally related organic impurities were successfully separated, and five of them were quantified. Ultimately, a purity of 94.90% was assigned to T4 CRM, with an expanded uncertainty of 0.34% ( k = 2).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
EUROPEAN POLYMER JOURNAL
·
2025
·
IF 6.3
Kun Li,Mohashin Kabir,Mahbubur Rahman,Shaojuan Chen
The development of advanced electrospun nanofiber yarns (ENYs) featuring highly aligned structures has garnered considerable attention, as these ENYs serve as fundamental building blocks for biomedical textile fabrication. In this work, we successfully fabricated highly aligned chitosan (CS)/poly(L-lactide-co-ε-caprolactone) (PLCL) ENYs through an innovative combination of electrospinning and thermal stretching techniques. Four CS/PLCL compositions were subjected to thermal stretching at 60 °C to generate 4 times stretching range, resulting in markedly improved fiber alignment and morphological characteristics. Mechanical characterization revealed an interesting trade-off: while Young’s modulus decreased post-stretching, elongation at break increased substantially, indicating enhanced material flexibility. Degradation studies demonstrated composition-dependent behavior, with higher CS content accelerating biodegradation rates. Notably, surface wettability was effectively tuned, as evidenced by water contact angle measurements decreasing dramatically from 130° to 69° with increasing CS content. Most importantly, the 1 % CS/PLCL formulation emerged as particularly promising, demonstrating exceptional antibacterial performance (86.2 % inhibition against E. coli ) coupled with outstanding human dermal fibroblast adhesion and proliferation. Comprehensive evaluation revealed that these highly aligned nanofibers, especially the 1 % CS/PLCL variant, achieved an optimal combination of structural properties (morphology and crystallinity), degradation kinetics, antibacterial efficacy, and biocompatibility.
✓ 已验证
产品关联
DOI
高影响因子
Biomaterials Advances
·
2025
·
IF 6
Chen Shen,Xingyu Zhou,Junhui Jiang,Junbo Dang,Tong Lin,Ruibo Hu,Tianhao Zhao,Dahui Sun,Mei Zhang
Soft tissue fillers have received much attention for the treatment of skin aging and the restoration of missing or excised soft volumes. However, the development of fillers with both safety and applicability remains a challenge. This study presented a synergistic composite filler comprising aldehyde-modified hyaluronic acid (OHA), hydrazide-modified hyaluronic acid (NHA), amino-modified poly-L-lactic acid microspheres (NPLLA), and antioxidant copper peptide. The hydrogel formed in situ via Schiff base reaction exhibited excellent mechanical properties and immediate filling effect. The amino-modified PLLA microspheres were less likely to agglomerate, avoiding the occurrence of adverse reactions and further enhancing the mechanical properties of the filler. The timed-release platform provided protection for copper peptide stability, scavenged reactive oxygen species during the fibrotic encapsulation phase, and synergistically promoted collagen deposition. In a nude mice skin aging model, hydrogels stimulated collagen production through the TGF-β/Smad signaling pathway with good long-term filler efficacy. Therefore, NPLLA microspheres/hyaluronic acid-based composite hydrogels exhibit great potential as injectable soft tissue fillers.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
·
2025
·
IF 5.4
Miao Yuan,Saidan Wang,Hongxia Du,Yating Sui,Xiangjing Zhang,Song Zhao,Qiushi Li,Shaojie Liu,Xuteng Xing,Erjun Tang
Two-dimensional Ti 3 C 2 T x MXene, with a large specific surface area, high chemical stability and excellent barrier properties, has become a promising of anti-corrosion filler in organic coatings. However, the poor dispersion and compatibility in organic coatings restrict its efficient application. To this end, L-histidine (L-His) corrosion inhibitor was grafted on the 3-aminopropyl triethoxysilane (APTES) functionalized Ti 3 C 2 T x MXene surface, under the electrostatic attraction between the carboxylate and protonated aminopropyl groups. The obtained APTES-Ti 3 C 2 T x MXene@L-His cannot only accelerate the dispersion of Ti 3 C 2 T x MXene filler, but also greatly promote the anti-corrosion property and service lifetime of coatings through combining the physical shielding effect of MXene laminate structure with active anti-corrosion function of L-His corrosion inhibitor. Results show that amino-functionalized Ti 3 C 2 T x MXene can maintain homogeneous in epoxy resin for 300 h, enhancing its dispersion in epoxy coating. Moreover, APTES-Ti 3 C 2 T x MXene@L-His composite can achieve the continuous release of L-His for 3000 min, and additional acid-base dual response property, the releasing rate of L-His is significantly accelerated in acidic or alkaline solutions. The total loading amount of L-His in APTES-Ti 3 C 2 T x MXene@L-His composite is about 3 %. Furthermore, the practical composite coating containing APTES-Ti 3 C 2 T x MXene@L-His also exhibits the excellent anti-corrosion and certain self-healing properties, such as its |Z 0.01 Hz| value remains above 10 10 Ω·cm 2 during the 40 d immersing in 3.5 NaCl solution, which is 3 orders of magnitude higher than that of pure epoxy coating. This work extends the great significance for Ti 3 C 2 T x MXene based composite coatings application of in the field of anti-corrosion.
✓ 已验证
产品关联
DOI
高影响因子
MOLECULES
·
2025
·Vol. 30
·期 11
·2400
·
IF 4.6
Ming Yuan,Huai Wang,Mingjun Yu,Sen Yao,Risheng Yao
Cisplatin (CIS) is a widely used chemotherapeutic agent that is highly effective against various cancers. However, its clinical application is frequently limited by its substantial nephrotoxic side effects. The gastrin-releasing peptide receptor (GRPR), a critical regulator in inflammatory diseases, has been identified as a promising therapeutic target. Our previous studies have demonstrated that the GRPR antagonists PD176252 and RH-1402 can mitigate CIS-induced nephrotoxicity through anti-inflammatory mechanisms. Based on these findings, we designed and synthesized a series of 2-arylpropanoic acid-L-tryptophan derivatives to enhance the therapeutic effects. Among these compounds,3mexhibited superior renal protection by significantly improving mouse renal tubular epithelial cell (mRTEC) viability from 50.2 ± 2.6% to 80.5 ± 3.9%, surpassing PD176252 (70.8 ± 1.4%) and RH-1402 (73.9 ± 3.7%). Moreover, compound3mmarkedly reduced the expression of kidney injury molecule-1 (KIM-1) and inflammatory cytokines [Tumor Necrosis Factor-α (TNF-α), Interleukin-6 (IL-6), Monocyte Chemoattractant Protein-1 (MCP-1)]. Finally, molecular docking results revealed that3mexhibited a high binding affinity for GRPR. Computational predictions using SwissADME further indicated that3mpossesses favorable drug-like properties, thereby supporting its potential as a promising candidate for mitigating CIS-induced nephrotoxicity.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Zhennan Cui,Yigui Qiu,Yiming Zheng,Ning Ou,Ziyan Zhang,Jia Li,Wen Gu
A total of 45 l-menthol derivatives with thiosemicarbazide, semicarbazide, or thiazole structures were designed and synthesized, and the in vitro inhibitory activities were evaluated against seven plant pathogens. Some title compounds exhibited remarkable antifungal activities. Specifically, compound 4n demonstrated a significant antifungal effect against Sclerotinia sclerotiorum with an EC50 value of 1.03 mg/L, outperforming thifluzamide (1.32 mg/L). Furthermore, at a concentration of 200 mg/L, 4n displayed excellent protective (97.5%) and curative (95.7%) effects on S. sclerotiorum-infected carrots. In the phytotoxicity experiment, 4n exhibited no effect on the normal germination of carrot and mung bean seeds. Fluorescence imaging revealed a significant accumulation of reactive oxygen species (ROS) in mycelia treated with 4n. Additionally, 4n exhibited substantial inhibitory effects on the sclerotium formation and germination of S. sclerotiorum. A further mechanism study revealed that 4n could dose-dependently increase the cell membrane permeability and significantly inhibit the defense/respiration-related enzymes of S. sclerotiorum. The transcriptome analysis suggested that 4n could notably affect the gene expression related to the oxidation–reduction process and oxidoreductase activity. The enzyme inhibition assay and molecular docking studies implied that 4n could be a potential succinate dehydrogenase (SDH) inhibitor. These findings indicated that this class of l-menthol thiosemicarbazide derivatives holds promise as potential fungicides for the prevention and treatment of postharvest sclerotinia rot.
✓ 已验证
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）