查找使用阿拉丁产品的科研文献

NIR-II fluorescence visualization of ultrasound-induced blood–brain barrier opening for enhanced photothermal therapy against glioblastoma using indocyanine green microbubbles

Simin Liang,Dehong Hu,Guofeng Li,Duyang Gao,Fei Li,Hairong Zheng,Min Pan,Zonghai Sheng

Focused ultrasound (FUS)-induced blood–brain barrier (BBB) opening is crucial for enhancing glioblastoma (GBM) therapies. However, an in vivo imaging approach with a high spatial–temporal resolution to monitor the BBB opening process in situ and synchronously is still lacking. Herein, we report the use of indocyanine green (ICG)-dopped microbubbles (MBs-ICG) for visualizing the FUS-induced BBB opening and enhancing the photothermal therapy (PTT) against GBM. The MBs-ICG show bright fluorescence in the second near-infrared window (NIR-II), ultrasound contrast, and ultrasound-induced size transformation properties. By virtue of complementary contrast properties, MBs-ICG can be successfully applied for cerebral vascular imaging with NIR-II fluorescence resolution of ∼168.9 μm and ultrasound penetration depth of ∼7 mm. We further demonstrate that MBs-ICG can be combined with FUS for in situ and synchronous visualization of the BBB opening with a NIR-II fluorescence signal-to-background ratio of 6.2 ± 1.2. Finally, our data show that the MBs-ICG transform into lipid-ICG nanoparticles under FUS irradiation, which then rapidly penetrate the tumor tissues within 10 min and enhance PTT in orthotopic GBM-bearing mice. The multifunctional MBs-ICG approach provides a novel paradigm for monitoring BBB opening and enhancing GBM therapy.

NIR-II-Triggered Composite Nanofibers to Simultaneously Achieve Intracranial Hemostasis, Killing Superbug and Residual Cancer Cells in Brain Tumor Resection Surgery

Yang Jun,Xu Lei,Ding Yining,Liu Chang,Wang Bingchang,Yu Yanchao,Hui Chao,Ramakrishna Seeram,Zhang Jun,Long Yunze

Malignant glioblastoma (GBM) is prone to relapse due to the inevitable tumor cells residue by surgery. During the tumor resection surgery in brain, addressing bleeding and superbug infections is also full of challenges. Currently, no method or material in clinical craniotomy can simultaneously solve these three problems. Herein, Chitosan composite nanofibers embedded with CuSe nanoparticles were prepared by green electrospinning method, in which the CuSe nanoparticles have strong absorption in the second near-infrared (NIR-II) window. Immediately after removing the tumor in craniotomy, nanofibers were electrospun and deposited directly onto the resection site with high precision (> 90%) to achieve rapid hemostasis (< 8 s). Moreover, evidenced by the deeper penetration depth of NIR-II light (1064 nm) both in the scalp and skull than NIR-I light (808 nm), photothermal and photodynamic therapy induced by NIR-II exhibits efficient superbug-killing rate (> 99%) and effectively induces cell apoptosis of residual tumor thereby to inhibit tumor recurrence. Only using the same material, a trilogy of intracranial hemostasis, killing superbug and residual cancer cells is simultaneously achieved. The short operation time reduces the risk of craniotomy. This electrospinning strategy could combine with craniotomy and minimally invasive surgery, which may provide novel perspectives in clinical operation besides craniotomy. Graphical abstract

Oxygen-Generated Hierarchical-Structured AuNRs@MnO2@SiO2 Nanocarrier for Enhanced NIR- and H2O2-Responsive Mild-Hyperthermia Photodynamic/Photothermal Combined Tumor Therapy

Wen-Jie Zheng,Ze-Lei Zhou,Qi-Yan Lv,Xiaojie Song,Wen-Xing Zhang,Hui-Fang Cui

Tumor hypoxia greatly restricts photodynamic therapy (PDT) efficiency. Photothermal therapy (PTT) implemented at high temperatures induces local skin burning and surrounding tissue damage. Herein, an near-infrared (NIR)- and H 2 O 2 -double responsive heat- and O 2 -generating core–shell–shell AuNRs@MnO 2 @SiO 2 nanocarrier with Au nanorods (AuNRs) as core and MnO 2 as sandwiched nanolayer is developed to realize enhanced photodynamic/photothermal combined tumor therapy at mild-hyperthermia temperatures. Coating poly- l -lysine on AuNRs is capable of preventing AuNRs from aggregation and facilitating the on-site growth of MnO 2 . The mesoporous SiO 2 nanoshell provides large loading sites for payload indocyanine green (ICG). The MnO 2 nanolayer greatly enhances the singlet oxygen ( 1 O 2 ) generation efficiency of ICG both in testing tubes and in cancer cells. Consequently, the O 2 -enhanced PDT endows the AuNRs@MnO 2 @SiO 2 -ICG nanoparticles (NPs) dramatically stronger antitumor effect both in vitro and in vivo. Their tumor growth inhibition ratio (67.6%) is significantly higher than that of AuNRs@SiO 2 -ICG (28.5%) after one dose intratumor injection and NIR irradiation. Together with their good biosafety, the AuNRs@MnO 2 @SiO 2 -ICG is promisingly translational. The multifunctionality should provide the AuNRs@MnO 2 @SiO 2 NPs a nanocarrier platform for various therapeutic agents to modulate the tumor microenvironment and enhance tumor therapy efficiency.

Unveiling the improved targeting migration of mesenchymal stem cells with CXC chemokine receptor 3-modification using intravital NIR-II photoacoustic imaging

Lin Yuejun,Zhou Hui-chao,Chen Ningbo,Ren Yaguang,Gao Rongkang,Li Qiaojia,Deng Yiwen,Han Xuejiao,Zhang Xiaoran,Xiang Andy Peng,Guo Bing,Liu Chengbo,Ren Jie

Background Therapy with genetically modified mesenchymal stem cells (MSCs) has clinical translation promise. Optimizing the targeting migratory ability of MSCs relies on accurate imaging of the distribution and extravasation kinetics of MSCs, and the corresponding imaging results could be used to predict therapeutic outcomes and guide the optimization of the treatment program. Among the different imaging modalities, second near-infrared (NIR-II) optical-resolution photoacoustic microscopy (OR-PAM) has merits, including a fine resolution, a deep penetration, a high sensitivity, and a large signal-to-background ratio. It would be an ideal candidate for precise monitoring of MSCs, although it has not been tested for this purpose so far. Results Penetrating peptide-decorated conjugated polymer nanoparticles (TAT-CPNPs) with strong NIR-II absorbance were used to label chemokine-receptor genetically modified MSCs, which were subsequently evaluated under intravital NIR-II OR-PAM regarding their targeting migratory ability. Based on the upregulation of chemokine (C-X-C motif) ligand 10 in the inflamed ears of contact hypersensitivity mice, MSCs with overexpression of corresponding receptor, chemokine (C-X-C motif) receptor 3 (Cxcr3) were successfully generated (MSC Cxcr3 ). TAT-CPNPs labeling enabled NIR-II photoacoustic imaging to discern MSC Cxcr3 covered by 1.2 cm of chicken breast tissue. Longitudinal OR-PAM imaging revealed enhanced inflammation-targeting migration of MSC Cxcr3 over time attributed to Cxcr3 gene modification, which was further validated by histological analysis. Conclusions TAT-CPNPs-assisted NIR-II PA imaging is promising for monitoring distribution and extravasation kinetics of MSCs, which would greatly facilitate optimizing MSC-based therapy. Graphical Abstract

NIR-induced self-healing and recyclable polyurethane composites based on thermally reversible cross-linking for efficient solar-to-thermal energy storage

Xiaoyi Li,Haibo Wang,Shunpan Yuan,Shaojian Lin,Sha Deng,Zongliang Du,Xu Cheng,Xiaosheng Du

Conventional chemically cross-linked phase change materials (PCMs) possess many merits, such as reliable structural stability, high mechanical strength , and excellent form stability, but they unable to be self-healed, recycled and reprocessed because of permanently covalent cross-linking structure. Furthermore, the poor solar-to-thermal performance of organic PCMs severely restricts their practical usage for solar energy utilization. Herein, novel dynamically covalent cross-linked PEG-based polyurethane (FMPCMs) with superior solar-to-thermal conversion performance, satisfactory self-healing ability and admirable recyclability were synthesized by introducing furan-decorated Ti 3 C 2 T x MXene nanosheets (f-MXene) into polyethylene glycol-based polyurethane (PEG-PU). f-MXene, which is dynamically and covalently linked in polymeric FMPCMs, served not only as an efficient photon capturer in PEG-PU for converting solar to thermal energy but also as a dynamic cross-linked point for thermal-induced recycling and self-healing. Due to the reversible Diels-Alder (DA) covalent bond , f-MXene nanosheets endows the FMPCMs with significantly increased near-infrared-induced (NIR-induced) self-healing efficiency (90.6%) and satisfactory recyclability. Furthermore, the solar-to-thermal conversion and storage efficiency of FMPCMs were effectively increased with the incorporation of f-MXene. In conclusion, FMPCMs show tremendous potential in solar-to-thermal energy storage.

NIR-to-Vis Handheld Platforms for Detecting miRNA Level and Mutation Based on Sub-10 nm Sulfide Nanodots and HCR Amplification

Yanli Zhu,Jikai Wang,Haitao Xie,Hailing Liu,Shuangquan Liu,Dongxiu He,Pengbing Mi,Suisui He,Jun Wang,Yiyang Sun

Sub-10 nm monodisperse alkaline-earth sulfide nanodots (ASNDs) with bright near-infrared (NIR)-excitation fluorescence and adjustable emission wavelength were prepared by a thermal decomposition method for the first time. The ASNDs exhibited high NIR-to-vis conversion efficiency and served as multicolor fluorescent labels in the proposed miR-224 assay. Targeted detection of the miR-224 level and single-nucleotide variation in miR-224 was carried out on a smartphone-based platform using a hybridization chain reaction (HCR) amplification strategy. In the presence of miR-224, the ASND-labeled HCR probes self-assembled on the surface of the diagnosis kits, generating strong fluorescent signals linearly proportional to miR-224 contents in the range of 10–2000 fM. Significantly, mutations in miR-224 led to the variation in the fluorescence intensity ratio in RGB channels. Simultaneously, evident changes of fluorescent brightness and color were easily visualized by the naked eye, which enabled on-site discrimination of miR-224 with different mutant loci. This work provides a novel preparation approach for ultrasmall NIR excitation sulfide nanodots and reveals the potential of the as-synthesized ASNDs in point-of-care (POC) nucleic acid testing. Further, it may provide a handheld platform for miRNA single-nucleotide polymorphism analysis.

Fabrication of graphene oxide/poly (L-lactide-co-ε-caprolactone) nanocomposite with NIR light-induced shape memory effect and antibacterial properties

Zou Yulong,He Qiaobai,Wang Shanrong,Zou Fang,Lu Xili,Sun Zhijie,Li Li

Poly(L-lactide-co-ε-caprolactone) (PLCL) nanocomposites reinforced with graphene oxide modified by poly (L-lactic acid) (g-GO) were prepared by solution casting, where the graft ratio of PLLA to GO is about 11.7%. The results show that the g-GO possesses improved dispersion and interface interactions with the PLCL matrix, therefore, the final mechanical properties of nanocomposites are enhanced. Comparing with neat PLCL, the tensile strength and elongation at break of (1wt%)g-GO/PLCL are increased by 86.4% and 82.4%, respectively. Moreover, the g-GO/PLCL nanocomposite containing 1 wt% g-GO exhibits excellent thermal- induced and NIR light-induced shape memory effects and the shape recovery ratio is all up to 95%. The nanocomposites also have favorable antibacterial properties. The g-GO/PLCL nanocomposites with good NIR light-induced shape memory effect, antibacterial properties, biodegradability and biocompatibility have great potential for minimally invasive surgery.

Novel Dual-Mode NIR-II/MRI Nanoprobe Targeting PD-L1 Accurately Evaluates the Efficacy of Immunotherapy for Triple-Negative Breast Cancer

Wan-Ling Liu,Yong-Qu Zhang,Xiang-Jie Luo,Yuan-Yuan Zhu,Liang Song,Zi-He Ming,Li-Xin Zhang,Meng-Jun Li,Rui-Chan Lv,Guo-Jun Zhang,Min Chen

Insight into the mechanism of intense NIR-to-red upconversion luminescence in Er3+ doped and Er3+–Yb3+ co-doped SrF2 nanoparticles

Qingling Zeng,Wenxiang He,Fangfei Luan,Yu Yan,Hongli Du,Juan Fu,Dongcai Guo

A succession of erbium single-doped and erbium/ytterbium co-doped SrF2 nanoparticles (NPs) were fabricated via a hydrothermal synthesis technique followed by high-temperature calcination. Structural, morphology, and element information of SrF2 NPs were explored using X-ray diffraction (XRD), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) instruments. Under near-infrared (NIR) excitation, the SrF2:Er3+ NPs exhibit a prominent red emission peak (at 670 nm) as well as two oppositely faint green emission peaks (at 525 nm and 548 nm). The probable mechanism of the dominant red up-conversion luminescence (UCL) emission was systematically studied by UC emission spectroscopy, Raman spectroscopy, Fourier transform-infrared spectroscopy (FT-IR) and diffuse reflectance spectrum analyses. We used the energy gap law to estimate the probability of electronic transition, which determines the UCL emission route. The behavior of the R/G value (emission intensity of red to green ratio) and the slope n (the number of photons absorbed during the excitation process) reflected the existence of a highly efficient cross-relaxation (CR) effect during the UC process in the SrF2 system, so we can infer that the prominent red UCL emission is due to the CR effect among adjacent erbium atoms. After co-doping with ytterbium ions, the red UCL emission intensity is further enhanced. By comparing 2 mol% Er3+,5 mol% Yb3+ co-doped SrF2 samples with a similar composition without ytterbium doping, a red UCL emission intensity enhancement of about 200 times was observed. This increase is ascribed to the existence of effective energy transfer up-conversion (ETUC) from ytterbium to erbium, and the presence of a large absorption peak of ytterbium in the range of 830–1060 nm was observed in the diffuse reflectance spectrum, indicating that ytterbium's ability to absorb 980 nm photon energy is significantly stronger than erbium's. The strong red radiation obtained for erbium/ytterbium co-doped SrF2 NPs is expected to have potential application prospects in in vivo bio-imaging and photodynamic therapy.

Ultrathin CuFe2S3 nanosheets derived from CuFe-layered double hydroxide as an efficient nanoagent for synergistic chemodynamic and NIR-II photothermal therapy

Sa Wang,Tingting Hu,Guanyun Wang,Zhengdi Wang,Dan Yan,Ruizheng Liang,Chaoliang Tan

Ultrathin two-dimensional (2D) nanosheets (NSs) have been widely explored as nanoagents for multimodal cancer therapies, such as photothermal therapy (PTT) combined with chemodynamic therapy (CDT). However, it is still a challenge to prepare 2D NSs with good performance in both CDT and NIR-II PTT. Here, we report the preparation of ultrathin 2D CuFe 2 S 3 NSs as an efficient nanoagent for synergistic CDT and NIR-II PTT to ablate cancer cells/tumors. Ultrathin 2D CuFe 2 S 3 NSs are first prepared by sulfurization of ultrathin CuFe-LDH NSs via a simple hydrothermal treatment. After modification with polyethylene glycol (PEG), the PEG-modified CuFe 2 S 3 NSs (CuFe 2 S 3 -PEG) show broadband NIR-II absorption and excellent photothermal conversion efficiency (~55.86%) at 1064 nm. Moreover, the overproduced glutathione in the tumor microenvironment can react with CuFe 2 S 3 -PEG NSs to release Fe 2+ and Cu + , thus activating Fenton reaction to efficiently produce hydroxyl radicals (·OH). Importantly, the photothermal effect of CuFe 2 S 3 -PEG could further synergistically improve the Fenton reaction by increasing the local temperature. In vitro and in vivo results reveal that the CuFe 2 S 3 -PEG has remarkable synergistic CDT/PTT antitumor activity. This study has proven that the metal chalcogenide NSs of CuFe 2 S 3 -PEG can be used as an efficient nanoagent for multimodal cancer therapies, which could also be promising in other applications like biosensors, drug delivery and antibacterial.

Polydopamine Coated PB-MnO2 Nanoparticles as an Oxygen Generator Nanosystem for Imaging-Guided Single-NIR-Laser Triggered Synergistic Photodynamic/Photothermal Therapy

Atheer Hameid Odda,Hailiang Li,Naveen Kumar,Naseeb Ullah,Malik Ihsanullah Khan,Gang Wang,Kuang Liang,Tan Liu,Yue-Yin Pan,An-Wu Xu

Exploring a combined phototherapeutic strategy to overcome the limitations of a single mode therapy and inducing high anticancer efficiency is highly promising for precision cancer nanomedicine. However, a single-wavelength laser activates dual photothermal/photodynamic therapy (PTT/PDT) treatment is still a formidable challenge. Herein, we strategically design and fabricate a multifunctional theranostic nanosystem based on chlorin e6-functionalized polydopamine (PDA) coated prussian blue/manganese dioxide nanoparticles (PB-MnO2@PDA-Ce6 NPs). Interestingly, the obtained PB-MnO2@PDA NPs not only offer an effective delivery system for Ce6 but also provide strong optical absorption in the near-infrared range, endowing high antitumor efficacy of PTT. More importantly, the as-prepared PB-MnO2@PDA-Ce6 nanoagents exhibit an effective oxygen generation, superior reactive oxygen species (ROS), and outstanding photothermal conversion ability to greatly improve PTT and PDT treatments. As a result, both in vitro and in vivo treatments guided by MR imaging on liver cancer cells reveal the complete cell/tumor eradication under a single wavelength of 660 nm laser irradiation, implying the simultaneous synergistic PDT/PTT effects triggered by PB-MnO2@PDA-Ce6 nanoplatform, which are much higher than individual treatment. Taken together, our phototherapeutic nanoagents exhibit an excellent therapeutic performance, which may act as a nanoplatform to find safe and clinically translatable routes to accelerate cancer therapeutics.

Analysis of the Isotopic Purity of D2O with the Characteristic NIR-II Phosphorescence of Singlet Oxygen from a Photostable Polythiophene Photosensitizer

Yunhe Lang,Shihong Wu,Qin Yang,Yanju Luo,Xia Jiang,Peng Wu

D2O plays important roles in a variety of fields (such as the nuclear industry and bioorganic analysis), and thus its isotopic purity (H2O contents) is highly concerned. Due to its highly similar physical properties to H2O and large excess amounts of H2O over D2O, it is challenging to distinguish D2O from H2O. On the basis of the characteristic NIR-II phosphorescence of singlet oxygen (1O2), and the fact that H2O is a more efficient quencher for 1O2 than D2O, here, we proposed to simply use the 1275 nm emission of 1O2 for the analysis of the isotopic purity of D2O. In normal cases (a xenon lamp for excitation), such steady-state emission is extremely weak for valid analytical applications, we thus employed laser excitation for intensification. To this goal, a series of photosensitizers were screened, and eventually polythiophene PT10 was selected with high singlet oxygen quantum yield (ΦΔ = 0.51), high H2O/D2O contrast, and excellent photostability. Upon excitation with a 445 nm laser, a limit of detection (LOD, 3σ) of 0.1% for H2O in D2O was achieved. The accuracy of the proposed method was verified by the analysis of the isotopic purity of several D2O samples (with randomly added H2O). More interestingly, the hygroscopicity of D2O was sensitively monitored with the proposed probe in a real-time manner; the results of which are important for strengthening the care of D2O storage and the importance of humidity control during related investigations. Besides D2O isotopic purity evaluation, this work also indicated the potential usefulness of the NIR-II emission of singlet oxygen in future analytical detection.