GLYCOCONJUGATE JOURNAL
·
2021
·Vol. 38
·期 5
·527-537
·
IF 2.916
Zhang Xin,Wang Chen,Han Qing,Chen Xuan,Li Guoyun,Yu Guangli
Mucins are the major proteins that distributed on the intestinal mucosa layer and protect the intestine from pathogens infection. The composition of intestinal mucin O -glycans can affect the health of the gastrointestinal tract in pigs. Porcine intestinal mucosa is widely used as the main raw material of heparin extraction. The heparin extraction residues rich in mucins were usually wasted. The structure of mucin derived O -glycans in porcine intestinal mucosa are currently unknown. In this study, we isolated the mucins from the heparin extraction residues and profiled the O -glycans. After heparin extraction, mucin was digested with trypsin, and separated by strong anion exchange chromatography. The mucin derived O -glycans were release by alkaline β elimination, and analyzed by ultra high performance liquid chromatography-porous graphitized carbon-Fourier transform mass spectrometry (UPLC-PGC-FTMS/MS). Thirty five kinds of O -glycans were identified, most of which were Core 3-derived glycans. In particular, the O -glycans containing sialic acid Neu5Ac accounted for 71.93% of the total O -glycans, which were different from that of other species, including mouse intestine, fish intestine, and porcine colon. The high content sialylated mucin may explain its effect in biological processes. Furthermore, the immunological activity results indicated that the porcine intestinal mucin could promote phagocytosis and proliferation without any cytotoxic effects, which may aid in the development of immunomodulators.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2021
·
IF 4.952
Chen Liu,Ping Yang,Haili Wang,Huanlu Song
Yogurt is widely acknowledged as a healthy dairy product with unique aroma. However, the changes in the odor compounds often affect the storage and shelf life of yogurt. Besides, the complex colloidal system and low concentration levels of most of odorants make it more difficult to be analyzed. Therefore, this study extracted the odor compounds of yogurt through four extraction methods, including dynamic headspace sampling (DHS), solid phase micro-extraction (SPME), solvent-assisted flavor evaporation (SAFE), and Stir bar sorptive extraction (SBSE). A total of 117 odorants, including 27 esters, 27 alcohols, 22 ketones, 16 acids, 15 aldehydes, 5 sulfides, and 5 other compounds, were identified. Of which, 31 odor-active compounds were perceived, and 12 compounds were smelled for the first time in yogurt. Of the four methods, DHS showed the most outstanding extraction effect by obtaining 88 odor compounds. Around 13 volatiles were characterized as the key odor-active compounds of yogurt. Similarly, acetophenone and butyl acrylate were detected as the key odor-active compounds of yogurt for the first time. 2,3-Butanedione was the vital odor-active compound with the highest flavor dilution (FD) factor in yogurt. The results concluded that the odor-active compounds in yogurt might have synergistic and masking effects between them.
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ACS Biomaterials Science & Engineering
·
2019
·
IF 4.511
Jianying Huang,Hui Yang,Jiajun Mao,Fang Guo,Yan Cheng,Zhong Chen,Xiaoqin Wang,Xiao Li,Yuekun Lai
Superwettable patterns with superhydrophobic and superhydrophilic units have the capacity of enriching and absorbing microdroplets for multifunctional biosensing. Combining the advantages of superwettable micropatterns and a rapid click reaction, we first prepared a film using propargyl methacrylate–ethylene dimethacrylate and then created a superhydrophobic–superhydrophilic micropattern by a rapid thiol–yne click reaction. Due to the high wettability contrast, water droplets tend to be anchored in the superhydrophilic region. Molecules dissolved in a water droplet are therefore uniformly enriched in the superhydrophilic region after evaporation because of the Malangoni effect. This provides a rational strategy to develop novel patterned microchips for sensing applications. Combining with fluorescence imaging technology, the Ti superwettable microchip can be used to detect o-phthalaldehyde in water, with a detection limit as low as 10–7 mol L–1. In addition, taking advantage of the oxidative color rendering ability of glucose, the microchip, when fabricated on a glass substrate, can realize reuseable glucose detection with a detection limit of 2 mM within 15 min.
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APPLIED ORGANOMETALLIC CHEMISTRY
·
2019
·Vol. 33
·期 4
·e4719
·
IF 3.259
Beibei Wang,Xiaoli Sheng,Yuming Zhou,Zhiying Zhu,Yonghui Liu,Xiao Sha,Chao Zhang,Huaying Gao
A novel strategy was proposed for the fabrication of high-performance acidic mesoporous poly ionic liquids catalyst. In this work, mesoporous poly ionic liquids (MPILs) were synthesized with P123 (PEO 20 PPO 70 PEO 20 ) served as pore-forming agent. Then, MPILs were treated with PW 3− anion exchange, thereby fabricating PW/MPIL-S(x). MPIL and PW/MPIL-S(x) were characterized by scanning electron microscope (SEM), transmission electron microscopy (TEM), Thermogravimetric analysis (TA), N 2 adsorption–desorption and Fourier transform infrared (FT-IR) spectra and X-ray photoelectron spectroscopy (XPS) spectra. The effect of solvent and concentration of P123 on the morphology and mesoporous structure of MPILs were investigated systematically. And the results show that MPILs were featured with mesoporous channel structure, high surface area (up to 737 m 2 /g) and large pore volumes (1.16 cm 3 /g), which benefit heterogeneous phase reaction (such as, alkylation of styrene with o -xylene). In the alkylation reaction, under optimal reaction conditions, the catalyst PW/MPIL-THF (4.0 g) shows high conversion of styrene (100%) and PXE yield (96.21%), demonstrating the excellent catalytic activities. Furthermore, PW/MPIL-S(x) are easy to be separated from the catalytic system by filtration and show no obvious decrease in catalytic activity after 6 cycle runs. The obtained PW/MPIL-S(x) catalyst exhibit high thermal and mechanical stability as well, indicating extensive application in high temperature acidic catalysis. This work might open up a new method for the synthesizing of porous polymer catalysts in the future.
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MICROCHIMICA ACTA
·
2019
·Vol. 186
·期 3
·1-9
·
IF 5.479
Ma Yujun,Yu Ying,Lin Bixia,Zhang Li,Cao Yujuan,Guo Manli
Copper nanoclusters (CuNCs) were synthesized starting from glutathione and copper(II) nitrate. They show blue fluorescence peaking at 432 nm when excited at 334 nm. In the presence of o -phenylenediamine (OPD), the blue fluorescence is decreased, but a yellow fluorescence appears with a peak at 557 nm. UV-visible absorptiometry, fluorometry and fluorescence lifetime measurements were used to show that OPD is oxidized by the small fraction of copper(II) ions present in the CuNCs to form the oxidized form of o -phenylenediamine (oxOPD) which displays weak yellow fluorescence, while the blue fluorescence decreases. The ratio of fluorescences at 557 and 432 nm increases linearly in the 0.15 to 110 μg·L −1 OPD concentration range, and the detection limit is 93 ng·L −1 . Compared to the method based on the use of dissolved Cu(II), the employment of CuNCs reduces the detection limit by a factor of 40. The method was applied to the determination of OPD in spiked environmental water and industrial wastewater samples. Recoveries ranged from 96.8 to 100.3%.
Schematic presentation of a ratiometric fluorometric method for detection of o-phenylenediamine (OPD) based on copper nanoclusters (CuNCs). OPD is oxidized by Cu 2+ present in CuNCs to form the oxidized form of o -phenylenediamine (oxOPD). FRET occurred between oxOPD and CuNCs, and the F 557 /F 432 value is amplified.
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JOURNAL OF HAZARDOUS MATERIALS
·
2019
·
IF 7.65
Lu-Lu Qu,Zhi-Qin Geng,Wen Wang,Kai-Chun Yang,Wei-Peng Wang,Cai-Qin Han,Guo-Hai Yang,Robert Vajtai,Da-Wei Li,Pulickel M. Ajayan
A three-dimensional (3D) substrate was developed by assembling a monolayer of graphitic carbon nitride (O-g-C 3 N 4 ) on Ag nanorod arrays (Ag NRs) for sensitive and recyclable surface enhanced Raman scattering (SERS) detection. The prepared Ag NRs/O-g-C 3 N 4 substrate not only generated a significant Raman enhancement effect as a result of the strong π-π stacking interaction between O-g-C 3 N 4 and the analytes but also possessed excellent self-cleaning property via visible-light irradiation that was attributed to its outstanding catalytic performance. Highly sensitive SERS detection could be achieved with a LOD of 8.2 × 10 −10 M for R6 G, and the substrate could be used repeatedly for at least four cycles with tolerable intensity attenuation. In addition, the 3D substrate exhibited long-term stability originating from the electron-donor effect of O-g-C 3 N 4 and high reproducibility due to the uniform decoration of O-g-C 3 N 4 on the Ag NRs through the strong interaction. Furthermore, using Ag NRs/O-g-C 3 N 4 , the recyclable detection of antibiotics in a water sample was demonstrated with high sensitivity, which indicates that the 3D Ag NRs/O-g-C 3 N 4 substrate is a promising candidate for eliminating the challenges of single-use SERS substrates and building a portable SERS platform to sense organic molecular species.
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ENERGY & FUELS
·
2019
·
IF 3.021
Zhiming Huang,Ping Li,Xiao Luo,Xia Jiang,Lei Liu,Fan Ye,Jiazhe Kuang,Yue Luo,Yuanzhu Mi
In the current study, novel environmentally friendly and interfacially active carbon nanotubes/SiO2 nanomaterials (CNTs/SiO2) for emulsified crude oil separation were prepared via grafting nano-SiO2 on the surface of oxidized carbon nanotubes (Ox-CNTs). The structure of the as-prepared CNTs/SiO2 was characterized by Fourier transform infrared spectroscopy, UV–visible absorption spectra, Raman spectra, X-ray diffraction spectra, thermogravimetric analysis, field-emission scanning electron microscope, and energy-dispersive X-ray spectroscopy. Besides, three-phase contact angles (θ) were employed to evaluate the wettability of CNTs/SiO2. Also, it was found that the θ value of CNTs/SiO2 was about 90°. The nanomaterials with interfacial activity can spontaneously assemble at the oil–water interface, thus improving the droplet coalescence in the emulsion. Afterward, the demulsification performance was evaluated in crude-oil emulsions using the bottle test, which demonstrated that the demulsification efficiency demulsification efficiency could reach 87.40%. Also, the water phase was clear, while the optimum concentration and sedimentation time were 500 ppm and 30 min, respectively. Furthermore, the possible demulsification mechanism was evaluated by an optical microscope. The findings in this work might provide a novel environmentally friendly and interfacially active nanomaterial as a crude oil demulsifier.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2019
·
IF 3.571
Shanshan Wang,Jiawen Fan,Lujing Xu,Kai Ye,Tong Shu,Songbai Liu
In this study, derivatization of epigallocatechin (EGC) by representative phytosterols (stigmasterol and β-sitosterol) was performed employing Steglich esterification. The structural identity and purity of epigallocatechin β-sitosterol (ESi) and epigallocatechin stigmasterol (ESt) were confirmed by NMR, FT-IR, and HPLC-MS. Further evaluation of ESi and ESt revealed their extraordinary antioxidant activities in O/W emulsion. Two different radical sources in oil or aqueous phase were applied to explore the antioxidant behavior in O/W emulsion. The mechanism was further investigated by fluorescent microscopy and transmission electron microscopy (TEM). Furthermore, incorporation of EGC with stigmasterol and β-sitosterol notably enhanced the cholesterol-reducing activity. TEM studies suggested the hydrogen bonding of EGC strengthened the aggregation network of ESi and ESt in the bile salt micelle. The exceptional properties of ESi and ESt signified their intriguing utilization in the food industry.
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ACS Applied Materials & Interfaces
·
2018
·
IF 8.097
Lulu Qu,Gen Zhu,Jie Ji,T. P. Yadav,Yijiang Chen,Guohai Yang,Hui Xu,Haitao Li
Organic pollutants are harmful to human health, which creates a global need for the development of novel and effective materials for efficiently removing contaminants. Accordingly, an efficient visible light-driven heterostructured membrane combined with oxygen-modified monolayer g-C3N4, graphene oxide, and nitrogen-doped carbon nanotubes (CNTs) (O-g-C3N4/GO/N-CNT) was successfully fabricated through electrostatic interactions and subsequent vacuum filtration. The results suggested that the O-g-C3N4/GO/N-CNT membrane exhibited higher degradation rate than those of O-g-C3N4/GO and pure O-g-C3N4 under visible light exposure. This enhanced photocatalytic performance was attributed to the introduction of GO and N-CNT, which acted as electronic acceptors for monolayer O-g-C3N4 that effectively inhibited recombination of photogenerated electron–hole pairs, thus enhancing visible light photocatalytic activity. Furthermore, the enrichment and degradation rates of O-g-C3N4/GO/N-CNT membranes were demonstrated for tetracycline hydrochloride, which were found to be 96.64 and 94.30%, respectively, and no distinct enrichment or catalytic activity reduction was observed when their reusability was measured. These results suggested that these recyclable O-g-C3N4/GO/N-CNT membranes provide a new strategy for the highly efficient removal of environmental pollutants.
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NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 21
·17644-17651
·
IF 3.201
Shaobo Li,Tao Zhu,Lichun Dong,Mingdong Dong
Heteroatom doping has been demonstrated to be an effective way to tune the properties of nanomaterials including chemical reactivity and electronic and optical performance. In this study, rGO/g-C3N4 and boron doped rGO/g-C3N4 (B-rGO/g-C3N4) nanocomposites were fabricated via a rapid plasma treatment method, and their photocatalytic performance under visible light radiation was examined. The results showed that the photocatalytic efficiency of B-rGO/g-C3N4 for rhodamine B degradation can be 50% higher than that of rGO/g-C3N4 at an optimum rGO loading of 5 wt%. XPS and FTIR analyses indicated the enhanced C–O–C covalent bonding between rGO and g-C3N4 in B-rGO/g-C3N4, and the optical characterization demonstrated that it could further improve the separation of the photo-generated chargers and narrow the bandgap of the nanocomposite. This work provides an idea that boron doping could be an effective way to promote the chemical interaction between different components in composite materials, which is important in designing and developing new functional nanomaterials.
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DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
·
2018
·
IF 1.883
Yi Chen,Qianming Du,Qianqian Guo,Jing Huang,Lina Liu,Xiangchun Shen,Jianqing Peng
The CaCO3 encapsulated liposome with pH sensitivity is an efficient carrier for the delivery of chemotherapeutic drugs. Herein, we provided an innovative method that take advantage of a W/O emulsion to prepare CaCO3 encapsulated liposomes for the delivery of curcumin. The liposomes with both CaCO3 and curcumin encapsulated (LCC) showed high sensitivity to reduced pH (the environment of lysosomes). Due to the inherent pH sensitivity of CaCO3, LCC swelled and released the encapsulated curcumin rapidly in acidic medium. The lysosome escape capability and promoted accumulation of curcumin in the cytosol from LCC was verified with respect to that of curcumin loaded liposomes (CLIPO). Despite the similar cytotoxicity within curcumin preparations in vitro at high concentration, LCC exhibited optimal antitumor effect in an azoxymethane (AOM)/dextran sodium sulfate (DSS)-induced colorectal cancer model, which was attributed to the long circulation time and efficient intracellular delivery of curcumin from LCC. It is suggested that the solubility and cytosolic delivery of curcumin are greatly improved by LCC, which accounts for the increased pharmacodynamic effect of curcumin. Thus, the CaCO3 encapsulated liposomes developed in this study is an ideal carrier for the hydrophobic drugs in potential clinical application.
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JOURNAL OF SEPARATION SCIENCE
·
2018
·Vol. 41
·期 22
·4185-4193
·
IF 2.415
Wenwu Yang,Turghun Muhammad,Aziguli Yigaimu,Kipayem Muhammad,Lingxin Chen
A novel magnetic molecularly imprinted polymer for the selective recognition of auramine O was rationally designed via screening from a library of nonimprinted polymers. A stoichiometric ratio of functional monomer (itaconic acid) and template molecule (auramine O) was found to be 1.5. Meanwhile, the synthesized SiO 2 @Fe 3 O 4 was modified by 0.5 mol/L hydrochloric acid to facilitate the preparation of magnetic molecularly imprinted polymer particles. Adsorption experiments showed that the magnetic polymer particles exhibited good selectivity, recoveries, and enrichment performance. The stoichiometric imprinted polymers have been employed for the selective preconcentration of auramine O from lake water sample. The high specificity of the stoichiometric imprinted polymers was proven in the extraction of mixture solution of auramine O, auramine O hydrochloride, and chrysoidine, and the recoveries ranged between 99.66 and 108.75% (RSD 2.6–3.7%, n = 3) for lake water. These results suggest that this method is effective and can be successfully applied to the analysis of auramine O in environmental water samples.
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