查找使用阿拉丁产品的科研文献

Alginate-based edible coating with magnoliae essential oil/β-cyclodextrin inclusion complex for Kyoho grape preservation

Yingjie Liu,Luo Weng,Sisi Dai,Minghui Liu,Xiufen Guo,Fanxin Meng

To prolong the shelf life of Kyoho grapes at room temperature, an edible coating composed of sodium alginate (SA) containing an inclusion complex of Magnoliae essential oil (MEO) and β-cyclodextrin (β-CD) (SA/MEO@β-CD coating) was developed. Initially, the inclusion complex of MEO with β-CD was developed to enhance its thermal stability. MEO@β-CD was characterized by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), and scanning electron microscopy (SEM). In addition, thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) results show that β-CD can improve the thermal stability of MEO. The entrapment efficiency and loading efficiency were 82.56% and 8.8%. MEO@β-CD was added to the SA coating for preserving Kyoho grapes, while monitoring the enzyme activity, total bacterial count, and sensory characteristics of the grapes. Compared to the uncoated group, the SA/MEO@β-CD coated group exhibited a significant reduction in total color difference, decay rate, weight loss rate, enzyme activity, and total number of bacterial colonies, while also slowing the decline in hardness and total soluble solids. The effect of the SA/MEO@β-CD1 group was highly effective. Conclusively, the SA/MEO@β-CD coating preserves Kyoho grapes by reducing lipid oxidation and inhibiting microbial growth, demonstrating its potential as an advanced edible packaging solution for grape preservation.

Development of Diselenide-Containing Poly(β-amino ester)s for Potent mRNA Delivery to the Spleen

Pingge Jiang,Yaxuan Wang,Qiong Wu,Ziyue Wang,Chengfan Wu,Qi Shuai,Yunfeng Yan

Polymer-based nanocarriers hold promise for nucleic acid delivery, yet the clinical translation of mRNA polymeric carriers remains hindered by insufficient delivery efficiency and cytotoxicity. Here, we synthesized 180 diselenide-containing poly(β-amino ester)s (PBAEs) via Michael addition. In vitro screening identified PBAEs with superior mRNA delivery efficiency, showing that hydrophobic amine backbones with ionizable cationic amine end-capping are critical. The effective mRNA-PBAE polyplex nanoparticles (PNPs) exhibited moderate positive charge, suitable size, potent cellular uptake, and endosomal escape. Compared with analogs lacking diselenide bonds, diselenide-containing PBAEs demonstrated robust redox responsiveness and formed PNPs with similar mRNA binding and size, but reduced internal hydrophobicity and improved cellular uptake, mediating potent mRNA delivery. Tail vein injection of diselenide-containing PNPs achieved robust splenic-targeted delivery in mice without helper lipids, unlike the lung-targeted delivery of conventional PBAEs. These findings highlight a novel diselenide-containing PBAE platform for effective mRNA delivery, which has great potential in mRNA-based immunotherapeutic applications.

Phospholipid/HP-β-CD Hybrid Nanosystems Amplify Neohesperidin Bioavailability via Dual Enhancement of Solubility and Stability

Na Xia,Qian Zhou,Yanquan Liu,Dan Gao,Siming Zhu,Zuoshan Feng

Neohesperidin (NH), a bioactive flavanone glycoside, exhibits multifaceted pharmacological properties including antioxidant and anti-inflammatory activities. However, its clinical application is severely constrained by inherent physicochemical limitations such as poor aqueous solubility and instability under physiological conditions. To address these challenges, this study developed a dual-carrier nano-liposomal system through the synergistic integration of phospholipid complexation and hydroxypropyl-β-cyclodextrin (HP-β-CD) inclusion technologies. Two formulations—NH-PC (phospholipid complex) and NH-PC-CD (phospholipid/HP-β-CD hybrid)—were fabricated via ultrasonication-assisted ethanol precipitation. Comprehensive characterization using FTIR and PXRD confirmed the amorphous dispersion of NH within lipid bilayers, with complete elimination of crystalline diffraction peaks, indicative of molecular-level interactions between NH’s hydroxyl groups and phospholipid polar moieties. The engineered nanosystems demonstrated remarkable solubility enhancement, achieving 321.77 μg/mL (NH-PC) and 318.75 μg/mL (NH-PC-CD), representing 2.01- and 1.99-fold increases over free NH. Encapsulation efficiencies exceeded 95% for both formulations, with sustained release profiles revealing 60.81% (NH-PC) and 80.78% (NH-PC-CD) cumulative release over 72 h, governed predominantly by non-Fickian diffusion kinetics. In vitro gastrointestinal simulations highlighted superior bioaccessibility for NH-PC-CD (66.35%) compared to NH-PC (58.52%) and free NH (20.85%), attributed to enhanced stability against enzymatic degradation. Storage stability assessments further validated the robustness of HP-β-CD-modified liposomes, with NH-PC-CD maintaining consistent particle size (<3% variation) and encapsulation efficiency (>92%) over 30 days. Antioxidant evaluations demonstrated concentration-dependent DPPH radical scavenging, wherein nanoencapsulation significantly amplified NH’s activity compared to its free form. This study establishes a paradigm for dual-functional nanocarriers, offering a scalable strategy to optimize the delivery of hydrophobic nutraceuticals while addressing critical challenges in bioavailability and physiological stability.

Stabilizing Bioactive Components in Fresh Spirulina via Hydroxypropyl-β-Cyclodextrin-Loaded Curcumin: A Functional Preservation Approach

Fan Zhihao,Shen Yu,Usman Muhammad,Su Kerui,Shahid Ayesha,Zhao Anqi,Li Xinyu,Zhang Beixiao,Xu Jingliang

Spirulina platensis is a nutrient-rich cyanobacterium with immune-enhancing, anti-aging, and cholesterol-lowering properties. However, fresh Spirulina is highly susceptible to spoilage during storage. This study developed a preservation strategy based on curcumin encapsulated in hydroxypropyl- β -cyclodextrin (HBC-Cur) to improve the storage stability of fresh Spirulina . During storage, treatment of fresh Spirulina with 2 mg/mL HBC-Cur exhibited protective effects by synergistically inhibiting microbial growth and mitigating oxidative reactions. Chlorophyll content was retained at 74.39% of the initial level. In terms of lipid and protein oxidation, malondialdehyde (MDA) content decreased by 45.73%, and protein carbonyl formation was reduced by 19.69%, indicating effective alleviation of oxidative deterioration. In addition, biogenic amines remained at consistently low levels, with putrescine content reduced by 76.53% relative to the control after 8 days. During the late storage phase, oxidative rancidity and spoilage-related VOCs, including hexanal, heptanal, nonenal, propionic acid, and nitrogen- and sulfur-containing volatiles, significantly increased in the control, whereas the VOCs of HBC-Cur-treated Spirulina remained relatively stable. This approach may offer a potential strategy for preserving bioactive components and suppressing the formation of spoilage-related compounds in Spirulina with implications for the development of sustainable food storage technologies. Graphical

Multicomponent tandem polymerization of alkynes, CO2, alkyl bromide, and thiol toward functional poly(β-thioacrylate)s

Mengchao Zhang,Sifan Li,Zhaohui Zhang,Hongkun Li

The direct conversion of carbon dioxide (CO2) into value-added polymeric materials has garnered considerable research interest in recent decades. Multicomponent tandem polymerization (MCTP), as an emerging synthetic strategy, demonstrates exceptional efficiency in constructing functional polymers. Nevertheless, CO2-involved MCTP systems have been rarely reported. Herein, we developed a one-pot MCTP involving CO2, alkynes, alkyl bromide, and thiol, enabling the efficient transformation of CO2 into structurally well-defined regioregular polymers. The polymerization process mediated by Ag2WO4 and Cs2CO3 under ambient pressure in N,N-dimethylacetamide upon heating produced four soluble poly(β-thioacrylate)s with high weight-average molecular weights (up to 11 500 g mol−1) and Z-stereoregularities (up to 84%). Notably, the tetraphenylethene-containing polymer exhibited aggregation-induced emission features, demonstrating its potential as a dopant in liquid crystalline polymer networks. The resulting film displayed circularly polarized luminescence properties with an exceptional luminescence dissymmetry factor of −0.88. Intriguingly, the polymer/polyamide 6 (PA6) composites manifested room-temperature phosphorescence featuring long emission lifetimes up to 510 ms. Furthermore, the polymers could be post-modified by the thiol–ene click reaction to produce sulfur-rich polymers. This work provides a robust platform for converting CO2 into stereoregular polymeric materials with tailorable photophysical properties, offering promising applications in advanced optoelectronic materials and sustainable technologies.

Preparation, stability and in vitro digestion of β-carotene-loaded emulsions stabilized by succinylated rice glutelin

Songyu Wang,Junjie Luo,Kefan Ouyang,Genyuan Li,Fangjie Xiao,Qin Zhang,Ningxiang Yu,Qiang Zhao

BACKGROUND β -Carotene (BC) is highly susceptible to degradation during processing, storage and digestion. This study evaluated BC-loaded emulsions stabilized by succinylated rice glutelin (S-RG) against whey protein isolate (WPI) emulsions in terms of stability and bioaccessibility. RESLUTS S-RG emulsions exhibited significantly smaller particle sizes than WPI emulsions at pH 5 and 6, indicating better colloidal stability in mildly acidic conditions. Both systems showed comparable resistance to thermal treatment, ultrasonication and UV exposure. However, during in vitro gastrointestinal digestion, the S-RG emulsion demonstrated superior functionality, achieving a fat release rate of 18.33 ± 0.89%, a BC bioretention rate of 52.5 ± 0.72% and a bioaccessibility of 21.61 ± 1.33% – all notably higher than those of the WPI control. CONCLUSION S-RG effectively protects BC and enhances its bioaccessibility, performing comparably or better than animal-derived WPI. These findings support S-RG as a sustainable, plant-based alternative for delivering sensitive lipophilic nutrients in functional food emulsions. © 2026 Society of Chemical Industry.

Immobilization of hydroaminase-expressing recombinant Escherichia coli whole-cell biocatalysts for the production of β-alanine

Ma Li,Wang Yuyu,Liu Ruiqi,Hu Jingjia,Zheng Xiaobing,Yue Xiaoyang,Liu Guanhua,He Ying,Zhou Liya

Developing stable and easy-to-operate biocatalysts is crucial for their use as industrial catalysts. Here immobilized whole-cell catalysts were used for β-alanine production by immobilizing recombinant Escherichia coli cells (containing hydroaminase) with diatomite. E. coli BL21 (DE3)/pET-30a ( +)-HAMase was genetically engineered for the efficient synthesis of β-alanine from acrylic acid and aqueous ammonia. Using glutaraldehyde as a cross-linking agent, polyethyleneimine (PEI) as a flocculant, and diatomite as the immobilization carrier, optimal immobilization was achieved with 8% (w/v) PEI solution, 5% (w/v) glutaraldehyde, and 100 mg wet cell/mL cell suspension, along with a PEI flocculation time of 2.5 h and glutaraldehyde cross-linking time of 1.5 h. The enzyme activity recovery rate reached 70.72%. Remarkably, the immobilized whole-cell catalysts exhibited excellent stability, retaining over 90% of initial enzyme activity after 12 h incubation at 45 °C and maintaining over 72% enzyme activity after storage for 60 days at 4 °C. Additionally, the immobilized cells demonstrated enhanced reusability, maintaining consistent β-alanine yield even after ten consecutive reaction batches with an average yield of approximately 80%.

Exposure to PFOA and GenX elicits cell typespecific impacts on p53 and TGF-β signaling pathways

Ding Hongran,Slack Maya,McClure Holly,Gu Wen,Gu Helene,Kappes Ferdinand,Schultz Thomas F.,Somarelli Jason A.,Tsigkou Anastasia

Per- and polyfluoroalkyl substances (PFAS) are a class of synthetic chemicals extensively used as plastic additives. Their environmental persistence and potential for bioaccumulation have raised significant toxicological concerns. This study evaluates the cytotoxic and molecular effects of Perfluorooctanoic acid (PFOA) and its replacement compound, Perfluoro(2-methyl-3-oxohexanoic) acid (GenX), in human-derived skin (A375), liver (HepG2), kidney (SN12C), and colon (SW620) cell lines. The experimental design assessed cell viability, gene expression, and perturbations in key cellular stress pathways, with a particular focus on TGF-β/SMAD-mediated inflammation and the p53-driven DNA damage response. Our results demonstrate compound- and cell-type-specific toxicity, with GenX displaying reduced cytotoxicity compared to PFOA across all cell types. Molecular analyses revealed that both PFAS induced alterations in the TGF-β/SMAD pathway, consistent with a pro-inflammatory cellular state. Additionally, we observed activation of the DNA damage response, as evidenced by increased expression of ATM, ATR, and p53, alongside ribosomal stress-related changes in RPL5 and RPL11. Notably, while skin and liver cells exhibited similar response profiles, kidney and colon cells showed divergent modulation of SMAD signaling, suggesting tissue-specific susceptibility and mechanistic differences. These findings contribute to a deeper understanding of the differential toxicological profiles of legacy and replacement PFAS, with implications for health risk assessment and regulatory policy. Graphical abstract

FNDC1 Competitively Binds Gβ2 to Suppress the β‐Catenin–Destruction Complex and Promote Gastric Cancer Malignancy

Wenyu Gao,Hao Chen,Fangyu Lin,Jialin Liu,Tingxuan Huang,Han Wang,Xiaojiao Weng,Xinli Wang,Xiaoyan Lin,Tao Jiang

Gastric cancer (GC) is a leading cause of cancer‐related deaths and has high recurrence rate. Although fibronectin domain‐containing protein 1 (FNDC1) is implicated in GC progression, its molecular mechanisms remain unclear. Multi‐omics analyses (TCGA, GEO datasets) were used to assess FNDC1 expression and clinical correlation. In vitro (cell proliferation, invasion, EMT markers) and in vivo (xenograft) experiments, combined with molecular assays (Co‐IP, WB, ChIP), explored FNDC1's function and mechanism. FNDC1 was significantly upregulated in GC, correlating with advanced clinicopathological features and poor prognosis. Knockdown of FNDC1 suppressed GC cell proliferation, invasion, and metastasis by inhibiting EMT and Wnt/β‐catenin signaling. Mechanistically, FNDC1 competitively bound the WD5 domain (residues 224–254) of Gβ2, disrupting Gβγ–Dvl1 interaction. This prevented Dvl1 degradation, promoted Axin1 ubiquitination, and destabilized the β‐catenin–destruction complex (GSK3 β–APC–Axin1), leading to β‐catenin accumulation and Wnt pathway activation. FNDC1 drives GC malignancy by targeting the Gβ2–Dvl1 axis to activate Wnt/β‐catenin signaling, suggesting FNDC1 as a novel prognostic biomarker and therapeutic target. FNDC1 competitively binds Gβ2 to suppress the β‐catenin–destruction complex and promote the malignant behavior of gastric cancer (GC) by the following mechanism: elevated FNDC1 competes with Dvl for binding to Gβ2, thereby reducing Dvl–Gβ2 interaction; this reduction inhibits Dvl degradation in the cytoplasm; increased Dvl recruits Axin1, thereby disrupting the β‐catenin–destruction complex (GSK3β–APC–Axin) and blocking ubiquitination and degradation of β‐catenin; accumulated β‐catenin in the cytoplasm translocates to the nucleus, where it activates Wnt target genes and promotes epithelial–mesenchymal transition (EMT) in GC cells.

WS₂–NH₂ Enhanced Fiber SPR Biosensor for Ultralow-Level β-Actin Detection via Cu–Glycine Functionalization

Chen Liu,Xue Zhou,Hanyu Bai,Yana Zhang,Yong Zhao,Xuegang Li

Beta-actin (ACTB), a highly conserved cytoskeletal protein, is frequently dysregulated in early-stage malignancies, making its sensitive and specific detection crucial for cancer screening. Here, we develop a reflective fiber-optic SPR biosensor functionalized with copper–glycine engineered WS₂ nanosheets for label-free ACTB detection. The Cu–glycine modification not only enables WS₂ surface amination for efficient antibody conjugation, but also introduces Cu²⁺-mediated near-infrared absorption to amplify the plasmonic resonance response. Combined with the intrinsically large surface area and high refractive index sensitivity of WS₂, this synergistic design markedly enhances both molecular binding and signal transduction. The resulting WS₂-NH₂-based sensor achieves a refractive index sensitivity of 1467.5 nm/RIU, representing a 1.67-fold enhancement over unmodified fiber probes. For ACTB detection, it delivers an ultralow detection limit down to 9.766 pM, a broadened linear range of 2.4–120 pM (5×expansion), and a biosensing sensitivity of 84.477 nm/nM (1.4× improvement). Furthermore, at 1 ng/mL, a rapid response time of 20 min is achieved, demonstrating applicability for trace-level biomarker screening. Integrating high sensitivity, broad dynamic range, amplified spectral response, and strong anti-interference capability, this plasmonic WS₂-NH₂-modified fiber platform enables highly selective, label-free ACTB quantification. These findings highlight its strong potential for early cancer diagnosis, biomarker monitoring, and precision medical applications.

Synthesis of (E)-β-iodovinyl sulfones via DTBP/I2 promoted difunctionalization of alkynes with sodium benzenesulfinates

Wenzhu Bi,Chunyan Ren,Linguo Jia,Xiaoyi Xia,Xi Chen,Xiaolan Chen,Yufen Zhao

β-Ga2O3 Nanostructures: Chemical Vapor Deposition Growth Using Thermally Dewetted Au Nanoparticles as Catalyst and Characterization

Asha Yadav,Bo Fu,Stephanie Nicole Bonvicini,Linh Quy Ly,Zhitai Jia,Yujun Shi

β-Ga2O3nanostructures, including nanowires (NWs), nanosheets (NSHs), and nanorods (NRs), were synthesized using thermally dewetted Au nanoparticles as catalyst in a chemical vapor deposition process. The morphology of the as-grownβ-Ga2O3nanostructures depends strongly on the growth temperature and time. Successful growth ofβ-Ga2O3NWs with lengths of 7–25 μm, NSHs, and NRs was achieved. It has been demonstrated that the vapor–liquid–solid mechanism governs the NW growth, and the vapor–solid mechanism occurs in the growth of NSHs and NRs. The X-ray diffraction analysis showed that the as-grown nanostructures were highly pure single-phaseβ-Ga2O3. The bandgap of theβ-Ga2O3nanostructures was determined to lie in the range of 4.68–4.74 eV. Characteristic Raman peaks were observed with a small blue and red shift, both of 1–3 cm−1, as compared with those from the bulk, indicating the presence of internal strain and defects in the as-grownβ-Ga2O3nanostructures. Strong photoluminescence emission in the UV-blue spectral region was obtained in theβ-Ga2O3nanostructures, regardless of their morphology. The UV (374–377 nm) emission is due to the intrinsic radiative recombination of self-trapped excitons present at the band edge. The strong blue (404–490 nm) emissions, consisting of five bands, are attributed to the presence of the complex defect states in the donor (VO) and acceptor (VGaor VGa–O). Theseβ-Ga2O3nanostructures are expected to have potential applications in optoelectronic devices such as tunable UV–Vis photodetectors.