ACS Applied Materials & Interfaces
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2023
·
IF 9.5
Zening Huang,Kaixiang Xu,Lijuan Zhao,Li-e Zheng,Nana Xu,Caixia Yan,Xingjiang Hu,Qiao Zhang,Jian Liu,Qingwei Zhao,Yaokun Xia
The development of a strategy for imaging of glutathione (GSH) and apurinic/apyrimidinic endonuclease 1 (APE1) in an organism remains challenging despite their significance in elaborating the correlated pathophysiological processes. Therefore, in this study, we propose a DNA-based AND-gated nanosensor for fluorescence imaging of the GSH as well as APE1 in living cells, animals, and organoids. The DNA probe is composed of a G-strand and A-strand. The disulfide bond in the G-strand is cleaved through a GSH redox reaction, and the hybridization stability between the G-strand and A-strand is decreased, leading to a conformational change of the A-strand. In the presence of APE1, the apurinic/apyrimidinic (AP) site in the A-strand is digested, producing a fluorescence signal for the correlated imaging of GSH and APE1. This nanosensor enables monitoring of the expression level change of GSH and APE1 in cells. Additionally, we illustrate the capability of this “dual-keys-and-locked” conceptual methodology in achieving specific tumor imaging when GSH and APE1 are present simultaneously (overexpressed GSH and APE1 in tumor cells) with improving tumor-to-normal tissue ratio in vivo. Furthermore, using this nanosensor, the GSH and APE1 also are visualized in organoids that recapitulate the phenotypic and functional traits of the original biological specimens. Overall, this study demonstrates the potential of our proposed biosensing technology in investigating the roles of various biological molecules involved in specific diseases.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2023
·
IF 8.6
HaoJun Tian,Jiangzhou Luo,Xiangyun Liu,Xueping Zong,Song Xue
PIM-1 thin film composite (TFC) membrane has attracted great attention for organic solvent nanofiltration (OSN) because of its high porosity and excellent solution process-ability. However, the PIM-1 thin selective layer may swell excessively in polar solvents such as methanol and acetone, and thus it makes the connection between the PIM-1 selective layer and the porous support unstable, which can significantly impair its separation performance. In this work, a series of novel PIM-1 TFC membranes was obtained by spin-coating of PIM-1/tetrahydrofuran solution onto α-Al 2 O 3 ceramic supports followed by a thermal treatment process. Since the nitrile group within PIM-1 tends to experience a thermal crosslinking reaction forming stable triazine rings after heat treatment, thus the organic solvent resistance ability of PIM-1 TFC membrane was greatly enhanced. Results showed that the thermally treated PIM-1 TFC membranes possess better chemical stability in polar solvents and acceptable permeances for various solvents (e.g., 2 ∼ 4.2 L·m −2 ·h −1 ·bar −1 for methanol, and 4.3 ∼ 6.8 L·m −2 ·h −1 ·bar −1 for toluene). For instance, the PIM-1 TFC membrane termed as PIM-1-3 wt%-1h TFC showed a methanol permeance of 3.8 L·m −2 ·h −1 ·bar −1 with a rejection of 96.5% towards Congo red (M w = 697 Da). Moreover, compared to the pristine PIM-1 TFC membrane, the thermally treated PIM-1 TFC membrane possesses better separation stability after the long-term static immersion test in acetone and DMF for 7 days, and it also exhibit good stability in the continuous filtration test using 20 mg·L −1 Congo Red methanol solution, demonstrating a promising outlook in OSN fields.
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International Journal of Electrochemical Science
·
2023
·
IF 1.5
Yong Zheng,Qian Wang,Yongjun Zheng,Zhen Wang,Dayong Tian
Electrolytic recovery of pure aluminum is a conventional method for the recycling of waste aluminum alloys, which is very important to economic development and environmental protection. However, the conventional electrolytic recovery process suffers from several inherent problems, which restricts the further development of this technology. In this study, an air- and moisture-stable 1-butyl-3-methylimidazolium bis(trifluoromethanesulfonyl)imide ionic liquid was applied in the dissolution of aluminum chloride. The upper phase of the resulting mixture was used as a low-temperature electrolyte in the electrolytic recovery of pure aluminum. The results of cyclic voltammetry and linear sweep voltammetry showed that aluminum could be electrodeposited from the electrolyte and that the main impurity elements of the aluminum alloy could also be stripped off by the electrolytic method. Based on these measurements, the electrolytic recovery process was conducted at 0.2−0.6 V and 303.2−373.2 K. Scanning electron microscope micrographs showed that smooth, compact and well-adherent deposits were obtained at 0.4 V from 303.2 to 323.2 K. In this situation, the preferred crystallographic orientation of these deposits was the (200) plane. Meanwhile, the mass content of aluminum in all deposits was higher than 99.6% according to the purity characterization. This study may be useful for the further application of ionic liquids in the low-temperature recycling of waste aluminum alloys.
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TOXICOLOGY LETTERS
·
2023
·
IF 3.5
Yufen Liao,Xin Wang,Guangyun Ran,Shiyu Zhang,Chutian Wu,Rong Tan,Ying Liu,Yan He,Ting Liu,Zhongxiu Wu,Ying Peng,Weiwei Li,Jiang Zheng
Columbin (CLB) is the most abundant (>1.0%) furan-containing diterpenoid lactone in herbal medicine Tinospora sagittate (Oliv.) Gagnep. The furano-terpenoid was found to be hepatotoxic, but the exact mechanisms remain unknown. The present study demonstrated that administration of CLB at 50 mg/kg induced hepatotoxicity, DNA damage and up-regulation of PARP-1 in vivo . Exposure to CLB (10 μM) induced GSH depletion, over-production of ROS , DNA damage, up-regulation of PARP-1 and cell death in cultured mouse primary hepatocytes in vitro . Co-treatment of mouse primary hepatocytes with ketoconazole (10 μM) or glutathione ethyl ester (200 μM) attenuated the GSH depletion, over-production of ROS, DNA damage, up-regulation of PARP-1, and cell death induced by CLB, while co-exposure to L -buthionine sulfoximine (BSO, 1000 μM) intensified such adverse effects resulting from CLB exposure. These results suggest that the metabolic activation of CLB by CYP3A resulted in the depletion of GSH and increase of ROS formation. The resultant over-production of ROS subsequently disrupted the DNA integrity and up-regulated the expression of PARP-1 in response to DNA damage, and ROS-induced DNA damage was involved in the hepatotoxicity of CLB.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2023
·
IF 5.2
Dingmei Han,Meiyu Liu,Chengming Huang,Xinyu Sun,Linjie Guan,Binbin He,Yi Mei,Yun Zu
Co-based catalysts are promising alternatives to replace expensive Pt- and toxic Cr-based catalysts for propane dehydrogenation (PDH), while the catalytic stability and propylene selectivity have been subjected to the diversity of Co speciations. Given the situation, uniformly stable hydroxylated Co 2+ silicate sites (depicted as {OH–Si–(OH)–Co–O–Si-(OH) 3 }) are successfully embedded into the silicalite-1 zeolite framework located in the straight and intersection channels (Co@S-1) with the assistance of Co precursors and geminal silanols via a facile hydrothermal synthesis , as revealed by detail characterizations. Under comparable conditions, a superior catalytic PDH performance with attractive propylene formation rate of 14.6 mmol·g cat −1 ·h −1 , propylene selectivity higher than 93% and very low deactivation rate of 0.0206 h −1 after 7 h is achieved over the Co@S-1(EDA) catalyst. Furthermore, the catalytic performance can be restored to the same as that of the fresh catalyst after 3 successive regenerations. In situ FTIR spectra analysis further reveals the dynamic changes in the characteristic absorption bands associated with the hydroxylated Co 2+ silicate sites and the intermediate product (2-propyl) during the PDH reaction and a reaction mechanism is proposed accordingly. This work provides a new insight for designing high-efficiency nonprecious metal PDH catalysts.
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ZEITSCHRIFT FUR KRISTALLOGRAPHIE-NEW CRYSTAL STRUCTURES
·
2023
·Vol. 238
·期 3
·541-543
·
IF 0.3
Chuanguo Cui,Jinxiao Zhang,Zhiyin Xiao
C 61 H 50 Cl 2 Fe 2 I 12 N 12 , orthorhombic, Pbcn (no. 60), a = 13.0247(4) Å, b = 17.0204(5) Å, c = 34.1475(9) Å, β = 90, V = 7570.0(4) Å 3 , Z = 4, R gt
( F ) = 0.0694, wR ref
( F
2 ) = 0.1605, T = 293(2) K.
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Microbiology Spectrum
·
2023
·Vol. 11
·期 3
·
IF 3.7
Jinchao Xing,Chen Hu,Siqi Che,Yixin Lan,Lihong Huang,Lele Liu,Youqin Yin,Huanan Li,Ming Liao,Wenbao Qi
Japanese encephalitis virus (JEV) is a typical mosquito-borne flavivirus that can cause central nervous system diseases in humans and animals. Host factors attempt to limit virus replication when the viruses invade the host by using various strategies for replication. It is essential to clarify the host factors that affect the life cycle of JEV and explore its underlying mechanism. Here, we found that USP1-associated factor 1 (UAF1; also known as WD repeat-containing protein 48) modulated JEV replication. We found that JEV propagation significantly increased in UAF1-depleted Huh7 cells. Moreover, we found that knockdown of UAF1 activated cell autophagic flux in further functional analysis. Subsequently, we demonstrated that autophagy can be induced by JEV, which promotes viral replication by inhibiting interferon-stimulated gene (ISG) expression in Huh7 cells. The knockdown of UAF1 reduced ISG expression during JEV infection. To explore the possible roles of autophagy in UAF1-mediated inhibition of JEV propagation, we knocked out ATG7 to generate autophagy-deficient cells and found that depletion of UAF1 failed to promote JEV replication in ATG7 knockout cells. Moreover, in ATG7-deficient Huh7 cells, interference with UAF1 expression did not lead to the induction of autophagy. Taken together, these findings indicate that UAF1 is a critical regulator of autophagy and reveal a mechanism by which UAF1 knockdown activates autophagy to promote JEV replication.IMPORTANCE Host factors play an essential role in virus replication and pathogenesis. Although UAF1 is well known to form complexes with ubiquitin-specific proteases, little is known about the function of the UAF1 protein itself. In this study, we confirmed that UAF1 is involved in JEV replication. Notably, we discovered a novel function for UAF1 in regulating autophagy. Furthermore, we demonstrated that UAF1 modulated JEV replication through its autophagy regulation. This study is the first description of the novel function of UAF1 in regulating autophagy, and it clarifies the underlying mechanism of the antiviral effect of UAF1 against JEV. These results provide a new mechanistic insight into the functional annotation of UAF1 and provide a potential target for increasing virus production during vaccine production.
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Small
·
2023
·Vol. 19
·期 28
·2301162
·
IF 13.3
Zewei Lian,Tingqing Wu,Huadong Wang,Jimei Chi,Lijun Cheng,Daixi Xie,Xiangyu Pan,Yuming Hu,Zhiyu Tan,Sisi Chen,Xu Yang,Yang Yun,Wei Wu,Chunbao Li,Meng Su,Yanlin Song
Rapid and ultra-sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is critical for early screening and management of COVID-19. Currently, the real-time reverse transcription polymerase chain reaction (rRT-PCR) is the primary laboratory method for diagnosing SARS-CoV-2. It is not suitable for at-home COVID-19 diagnostic test due to the long operating time, specific equipment, and professional procedures. Here an all-printed photonic crystal (PC) microarray with portable device for at-home COVID-19 rapid antigen test is reported. The fluorescence-enhanced effect of PC amplifies the fluorescence intensity of the labeled probe, achieving detection of nucleocapsid (N-) protein down to 0.03 pg mL −1 . A portable fluorescence intensity measurement instrument gives the result (negative or positive) by the color of the indicator within 5 s after inserting the reacted PC microarray test card. The N protein in inactivated virus samples (with cycle threshold values of 26.6–40.0) can be detected. The PC microarray provides a general and easy-to-use method for the timely monitoring and eventual control of the global coronavirus pandemic.
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CHINESE JOURNAL OF CHEMICAL ENGINEERING
·
2023
·
IF 3.8
Jinpei Huang,Xingwei Lu,Xuejing Zhang,Yiqiang Jin,Yifeng Zhou
1-Oxa-2-azaspiro [2.5] octane, as one of N-H oxaziridines , is a selective electrophilic aminating agent for N-, S-, C-, and O-nucleophiles. It has the features of stereoselectivity and the absence of formation of strongly acidic or basic byproducts, leading to considerable interest in the development of organic synthetic methods. Currently, the economically feasible route of production of 1-oxa-2-azaspiro [2.5] octane is the reaction of cyclohexanone with ammonia and sodium hypochlorite. However, due to strong exothermic reactions, massive gas release and heterogeneous reaction, the controllability, efficiency and safety of the reaction are in great difficulty using batch technology. In this paper, a microreaction system containing predispersion, reaction and phase separation was introduced into the preparation of 1-oxa-2-azaspiro [2.5] octane. The research results showed that precise control of the process including droplet dispersion, temperature control, reaction time control and fast continuous phase separation, was the key to process intensification. Under optimal conditions, the concentration of 1-oxa-2-azaspiro [2.5] octane in product obtained by microreaciton system (∼2.0 mol·L −1 ) was much higher than that obtained by batch technology (0.2–0.4 mol·L −1 ), which demonstrated that the continuous-flow synthesis would be a more efficient substitute for batch synthesis. Meanwhile, the results of the derivation experiments also showed that the aminating agent solution with higher concentration was more advantageous in the applications.
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Molecular Catalysis
·
2023
·
IF 4.6
Yuqing Wang,Miao Jiang,Li Yan,Cunyao Li,Guoqing Wang,Wenxiu He,Yunjie Ding
A series of porous organic polymer with different concentrations of tri(4-vinylphenyl)phosphite (P(OPh) 3 ) moieties were synthesized through the solvothermal synthesis technique. Their performance were investigated in 1-butene hydroformylation after metalation with metal species. The effect of the concentration of P(OPh) 3 moieties and pore structure of the polymers on catalytic efficiency were investigated in detail. The polymer-based Rh catalysts demonstrated a decrease in catalytic activity and inverse trends in selectivity with the increasing concentration of P(OPh) 3 . More importantly, Rh/POP-PhPh 3 -BP-P(OPh) 3 -x catalysts containing biphephos moieties exhibited more excellent catalytic performance than the corresponding Rh/POP-PhPh 3 -P(OPh) 3 -x catalysts. And the optimal catalytic performance, a valeraldehyde n/i ratio of 60.4, a valeraldehyde TOF of 9589.9 h −1 , and good catalytic stability were acquired for Rh/POP-PhPh 3 -BP-P(OPh) 3 –0.9 catalyst. The extraordinary catalytic properties are attributed to the formation of abundant active pentacoordinated hydridorhodium complexes, as demonstrated by in situ FT-IR experiments.
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MATERIALS CHEMISTRY AND PHYSICS
·
2023
·
IF 4.6
Yatao Pan,Guangjing Hou,Xiaojuan Wang,Xia Ran,Pingan Liu,Lijun Guo
Developing novel inhibitors for the fibrillization of amyloid peptides and understanding the relevant assembly processes are essential for biomedical and biomaterial sciences. Herein, we have synthesized and characterized the nitrogen and fluorine co-doped graphene quantum dots (N, F-GQDs), and investigated their inhibition of the fibrillization of Amyloid-beta peptide (1–42) (Aβ 42 ) by monitoring the assembly morphologies in the Aβ 42 /N, F-GQDs systems. Our results indicate that the N, F-GQDs functionalized with multiple surface groups effectively inhibits the fibrillization of amyloid Aβ 42 . The strong adsorption of Aβ 42 on N, F-GQDs significantly reduces the generation of β-sheet structures and the cytotoxicity of Aβ 42 /N, F-GQDs aggregates. Accordingly, a morphological evolution from the long fibrils of Aβ 42 to the spherical particles and unique closed-loop structures of Aβ 42 /N, F-GQDs have been captured. A possible interpretation for the observed phenomena in the presence of N, F-GQDs has been proposed by combining the different inhibition effects of other GQDs on the fibrillization of Aβ 42 and the results from various control experiments. Moreover, the inhibition activity of N, F-GQDs demonstrates good adaptability to resist the fluctuation of pH value and the interference of divalent metal ions , facilitating their practical applications in the future.
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DIGESTIVE DISEASES AND SCIENCES
·
2023
·Vol. 68
·期 6
·2414-2426
·
IF 3.1
Lin Mengjuan,Hu Guiying,Wang Zhengqiang,Yu Baoping,Tan Wei
Background The mechanism underlying irritable bowel syndrome (IBS), a common disease with hyperalgesia, remains elusive. The spinal cholinergic system is involved in pain modulation, but its role in IBS is unknown. Aims To determine whether high-affinity choline transporter 1 (CHT1, a major determinant of the cholinergic signaling capacity), is implicated in spinal modulation of stress-induced hyperalgesia. Methods A rat IBS model was established by water avoidance stress (WAS). Visceral sensations were detected by abdominal withdrawal reflex (AWR) and visceromotor response (VMR) to colorectal distension (CRD). Abdominal mechanical sensitivity was determined by von Frey filaments (VFFs) test. RT-PCR, Western blot, and immunostaining were performed for spinal CHT1 expression. Spinal acetylcholine (ACh) was measured by ELISA; the influence of spinal CHT1 on hyperalgesia were evaluated by intrathecal administration of MKC-231 (a choline uptake enhancer) and hemicholinium-3 (HC-3, a specific inhibitor of CHT1). Minocycline treatment was used to explore the role of spinal microglia in hyperalgesia. Results After 10 days of WAS, AWR scores and VMR magnitude to CRD, and the number of withdrawal events in VFF test were increased. Double-labeling showed that CHT1 in the dorsal horn was expressed in most of the neurons and almost all the microglia. The CHT1 expression and ACh levels in the spinal cord and the density of CHT1-positive cell in the spinal dorsal horn were enhanced in WAS-exposed rats. HC-3 enhanced pain responses in WAS rats; MKC-231 alleviated pain in WAS rats by upregulating CHT1 expression and increasing ACh production in the spinal cord. Furthermore, microglial activation in the spinal dorsal horn promoted the stress-induced hyperalgesia, and MKC-231 achieved analgesic effects by inhibiting the spinal microglial activation. Conclusions CHT1 exerts antinociceptive effects in spinal modulation of chronic stress-induced hyperalgesia by increasing ACh synthesis and suppressing microglial activation. MKC-231 has potential for treating disorders accompanied by hyperalgesia.
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