Food Bioscience
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2025
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IF 5.9
Chenxi Gu,Liyan Yang,Susu Han,Dengfeng Yang,Xiaodong Xu,WenChao Zhang,Mingguo Jiang,Lixia Pan
Cold-active chitinases that maintain robust catalytic performance at low temperatures are gaining attention as eco-friendly biocatalysts for energy-saving bioprocesses. In this study, a novel multifunctional chitinase of GH18 ( Cm Chi18B) was successfully cloned from a newly identified mangrove-associated bacterium Chitinibacter mangrovi FCG-7 T in Escherichia coli . The enzyme Cm Chi18B demonstrated adaptability to cold environments, retaining 55% of its maximal activity at 0°C. It displayed optimal activity at 55°C and pH 5.5. Cm Chi18B demonstrated high activity across a broad pH range of 4.0-12.0. Substrate hydrolysis analysis indicated that Cm Chi18B possesses bi-functional capabilities, including exochitinase and N-acetyl-β-D-glucosaminidase activities. After 24 h of incubation with colloidal chitin at 30°C, the enzyme achieved a N-acetyl-D-glucosamine (GlcNAc) conversion rate of 91.15% with a purity exceeding 98%. Intriguingly, the hydrolysis time for GlcNAc production varied with temperatures, with 0°C significantly impairing N-acetyl-β-glucosaminidase activity. These attributes position Cm Chi18B as a promising, low-energy biocatalyst for the sustainable production of GlcNAc and the valorization of chitin waste streams.
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INTERNATIONAL IMMUNOPHARMACOLOGY
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2014
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IF 2.711
Xiaomin Xia,Chuanyang Su,Juanli Fu,Pu Zhang,Xiaoji Jiang,Demei Xu,Lihua Hu,Erqun Song,Yang Song
This study investigated the protective effect of α-lipoic acid (LA) on lipopolysaccharide (LPS)/ d -galactosamine ( d -GalN)-induced fulminant hepatic failure in mice. First, we found that LA markedly reduced LPS/ d -GalN-induced increases in serum ALT and AST activities, which were supplemented with histopathological examination, suggested that LA has a protective effect on this model of hepatic damage. Livers challenged with LPS/ d -GalN exhibited extensive areas of vacuolization with the disappearance of nuclei and the loss of hepatic architecture. On the contrary, these pathological alterations were ameliorated by LA treatment. Next, we found that ROS and TBARS levels were increased in LPS/ d -GalN treated liver homogenates , which were attenuated by LA administration. Consistently, decreases in hepatic CAT and GPx activities were observed in LPS/ d -GalN group and were significantly restored by LA administration. Moreover, pretreatment with LA markedly reduced LPS/ d -GalN-induced iNOS , COX-2, TNF-α, NF-κB, IL-1β and IL-6 expressions. Furthermore, our data showed that TUNEL-positive cells increased in LPS/ d -GalN-treated mice liver which was counteracted by LA administration. LPS/ d -GalN induced apoptosis of hepatocytes, as estimated by caspase 3, caspase 8 and caspase 9 activations. Also, the increasing of Bax and the decreasing of Bcl-2 expressions also supported LPS/ d -GalN induced apoptosis. Interestingly, LA marked relieved these apoptotic features. Taking together, our results indicated that LA plays an important role on LPS/ d -GalN-induced fulminant hepatic failure through its antioxidant, anti-inflammatory and anti-apoptotic activities.
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DYES AND PIGMENTS
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2025
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IF 4.2
Zewei Song,Shuang Li,Yijie Zou,Yinghan Li,Xun Sun,Shouzhi Pu,Zhao Chen
Three D-A-A′ architecture isomeric luminogens ( o -, m -, p -DMAc-Ph 2 CO-BT ) were designed and synthesized, comprising acridine (donor, D), benzophenone (acceptor, A), and trifluoromethylphenol (acceptor, A′) units. Impressively, all three prepared compounds achieved concentration-dependent high-quality white-light emission, which resulted from the integration of bluish-violet single molecules and yellow excimers. Furthermore, solid p -DMAc-Ph 2 CO-BT exhibited reversible mechanofluorochromism (MFC), with a high-contrast fluorescence shift from blue-green to yellow-green upon grinding, rendering it suitable for a rewritable medium. Single-crystal structural analysis revealed the underlying mechanism of force-responsiveness among the isomers that only o -DMAc-Ph 2 CO-BT possessed C–H⋯F interactions, accompanied by diverse and shorter intermolecular contacts, leading to rigid stacking and MFC passivity. In contrast, p -DMAc-Ph 2 CO-BT showed the weakest intermolecular interactions and the most loosely packed structure, endowing it with high sensitivity to mechanical force. This study demonstrated the critical role of intermolecular interactions in modulating the MFC, providing valuable insights for the rational design of intelligent fluorescent materials.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2026
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IF 9.1
Zhaoguo Zhang,Dan Mei,Noor Ul Afsar,Yanan Chen,Jin Tang,Ke Liu,Jawad Ali,Dong Wang
Advanced membrane design with high proton selectivity is essential in industry for effective acid recovery and the elimination of multivalent metal ions, thereby reducing chemical consumption and environmental impact. Here, we present Vinyl carbazole (Vc)-functionalized membranes with adjustable interchain spacing (d-spacing) and hydrophilic channels to enhance acid recovery via diffusion dialysis. Adjusting Vc content optimizes the membrane structure for balanced proton conduction and metal-ion transport rejection. Moderate Vc incorporation (2–8 mol%) expands interchain spacing and simultaneously facilitates in situ crosslinking, thereby forming dense networks that suppress water uptake. This form of interconnected ionic channels significantly increases U acid flux: U acid values are 18.21 × 10 −3 m h −1 (HCl/FeCl 2 ) and 14.02 × 10 −3 m h −1 (H 2 SO 4 /FeSO 4 ), while metal ion transport (U salt ) is suppressed to 2.85 × 10 −6 m h −1 (HCl/FeCl 2 ) and 4.5 × 10 −6 m h −1 (H 2 SO 4 /FeSO 4 ). In the HCl/FeCl 2 system, the membrane achieved high selectivity (S ≈ 997.81–4515.99). However, further increasing the Vc content up to 10 mol% (PT 0.9 -Vc 0.1 ) enhances crosslinking, which reduces selectivity (S ≈ 1612.59) and proton flux (U acid = 9.20 × 10 −3 , and 8.86 × 10 −3 m h −1 for HCl/FeCl 2 and H 2 SO 4 /FeSO 4 ). These findings suggest that moderate Vc levels optimize high H + conductivity and prevent metal-ion crossover through steric effects, Donnan exclusion, and charge-assisted hopping via the carbazole nitrogen, thereby controlling Fe 2+ crossover. The adjustable microstructure also minimizes swelling, creating durable membranes suitable for industry use.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2026
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IF 9.6
Shixuan Yu,Shiying Fan,Shaomin Liu,Xinyong Li
The direct photocatalytic oxidation of inert methane (CH 4 ) to high-value-added oxygenates remains a significant challenge. This study precisely controls generated reactive oxygen species (ROS) types by modulating interfacial electronic interactions in Zn-Pt δ− -PS photocatalysts, promoting CH 4 conversion into formaldehyde (HCHO). Under ambient conditions, a HCHO production rate reached 3308 μmol·g −1 with a high selectivity of 77%. In situ spectroscopy and theoretical studies demonstrated that the elevated Pt d-band center in Pt-*OOH (* represents the adsorption state) lowered the occupancy of antibonding orbitals, thereby enhancing the interaction between Pt and *OOH, markedly polarizing the O O bond, facilitating its cleavage, and enabling the preferential in situ generation of •OH rather than •OOH. Furthermore, adequate •OH radical accelerated activation of the C H bond in CH 4 and induced the cleavage of its O H bond in methanol (CH 3 OH), promoting HCHO formation. This strategy may guide the rational design of the catalyst's electronic structure to enhance CH 4 photocatalytic oxidation. Download: Download high-res image (175KB) Download: Download full-size image
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Food Bioscience
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2026
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IF 6.2
Yucheng Li,Bo Fan,Yu-Cai He
E. coli P8 expressing a glycosyltransferase from Panax ginseng was constructed. • Coupling glycosyltransferase and sucrose synthase (SS) was adopted to yield Reb D. • Regeneration of UDPG in situ from UDP and sucrose was used to convert Reb A to Reb D. • DES (ChCl:glycerol=1:2) –H 2 O (4:6, v/v) enhances the conversion of Reb A to Reb D. • P8 cell and SS could convert 19.3 g/L Reb A to Reb D (21.0 g/L) in 8 h. Rebaudioside A (Reb A) can be converted into Rebaudioside D (Reb D) through a glycosylation reaction. Reb D is a higher-value sweetener with a very low caloric value, a glycemic index of zero, and greater sweetness than Reb A. However, its naturally low abundance limits large-scale production. Accordingly, this study developed an efficient bioconversion system for Reb D production. Two recombinant Escherichia coli strains were constructed and designated E. coli P8 and E. coli SS. E. coli P8 expressed a glycosyltransferase derived from Panax ginseng , whereas E. coli SS expressed a sucrose synthase derived from Arabidopsis thaliana . In the coupled system, sucrose synthase used sucrose and UDP to regenerate UDP-glucose (UDPG) in situ, while the glycosyltransferase catalyzed the conversion of Reb A into Reb D. A deep eutectic solvent (DES) composed of choline chloride and glycerol at a ChCl:Gly molar ratio of 1:2 was mixed with water to form a DES–H 2 O reaction medium. Under the selected conditions of pH 7.5, 45 °C, and a DES-to-water volume ratio of 40:60, P8 whole cells and an SS crude lysate converted 19.3 g/L Reb A into 21.0 g/L Reb D within 8 h using 500 mmol/L sucrose and 10 mmol/L UDP as co-substrates, corresponding to a molar yield of 93%. This study demonstrates an efficient and potentially environmentally friendly strategy for producing Reb D and other steviol glycosides. Download: Download high-res image (402KB) Download: Download full-size image
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JOURNAL OF CHROMATOGRAPHY A
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2025
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IF 4
Yan Zhang,Yaqian Zhou,Ping Liu,Yufeng Zhang,Yiqun Wan,Fan Liu,Genlin Sun,Lihua Chen,Jun Wang
A rapid and efficient method is highly desired for the determination of phenylurea herbicides (PUHs) in edible and medicinal herbs. Herein, we report a dispersive solid-phase extraction (d-SPE) method using a metal-organic framework Zn 4 O(NTB) 2 as the adsorbent. The microporous Zn 4 O(NTB) 2 can selectively extract targets while excluding matrix substances. Under optimized conditions, this method exhibits high sensitivity, with a limit of detections (LODs) and limits of quantification (LOQs) in the range of 0.09–0.32 ng mL -1 and 0.32–1.08 ng mL -1 , respectively. Moreover, the recoveries reach the range of 69.8 %-83.3 % with relative standard deviations below 6.2 %. The optimized adsorption efficiency of PUHs can be rapidly achieved in a short time of 10 min. Additionally, the adsorbent can be facilely regenerated with methanol washing. Furthermore, computational simulations reveal the guest-host interactions involving hydrogen bonding, π-π stacking interactions, and Van der Waals interactions. Practically, the established method has been successfully validated for extracting PUHs from Dendrobium candidum and Ganoderma lucidum samples.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Jing Gao,Hao Dong,Peichao Zhang,Kunpeng Gao,Haipeng Su,Jianan Sun,Xiangzhao Mao
Whole-enzyme cascade degradation of crystalline α-chitin is a long-term pursuit, which has been hampered by low transformation efficiency and heterogeneous products. Here, a novel β-N-acetylglucosaminidase (NAGase) from Sulfuriroseicoccus oceanibius, designated Sohex, was biochemically characterized. The enzyme demonstrated high hydrolytic activity toward N-acetyl chitooligomers (N-acetyl COSs) and exhibited promotive effects on crystalline α-chitin degradation. Furthermore, a whole-enzyme cascade degradation system based on lytic polysaccharide monooxygenases (LPMOs), chitinases, and Sohex was developed for N-acetyl-D-glucosamine (GlcNAc) production. This system achieved a maximum conversion rate of 45.93%, with GlcNAc purity reaching 99.99%. The addition of Sohex significantly enhanced α-chitin degradation efficiency, achieving conversion rates of 1.37-fold and 2.46-fold compared to the LPMOs + chitinases system and the sole chitinases system, respectively. Moreover, the promotion mechanisms of Sohex in conjunction with LPMOs and chitinases were elucidated, making the cascade system a promising strategy for converting crystalline α-chitin into high-purity GlcNAc.
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GREEN CHEMISTRY
·
2026
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IF 9.2
zonghao xu,Zongyu Guan,Yan Song,Xiran Zhao,Yaodong Yuan,Zhipeng Yan,Junhang Tian,Xueyi Sun,Biwei Xiao,Weidong Zhuang
Direct recycling of spent LiFePO₄ (LFP) cathodes is a sustainable and energy-efficient strategy for recovering valuable materials while preserving the intrinsic olivine structure. Herein, we report a LiI-assisted regeneration approach that achieves electronic structure reconstruction and reinforced Fe–O d–p orbital hybridization, enabling simultaneous repair of structural defects and enhancement of Li⁺ transport kinetics. The reductive I⁻ ions promote Fe³⁺→Fe²⁺ conversion and effectively eliminate Fe–Li antisite defects, while Li⁺ replenishment restores the stoichiometry of degraded LFP. Density functional theory (DFT) calculations reveal that LiI triggers a downward shift of the Fe 3d band center and an upward shift of the O 2p band center, narrowing their energy separation and strengthening Fe–O covalency. These electronic modifications reduce Li⁺ migration barriers and reconstruct continuous Li⁺ transport channels, as further validated by ab initio molecular dynamics simulations. In-situ XRD measurements confirm reduced unit-cell volume fluctuations and enhanced FeO₆–PO₄ framework stability, demonstrating improved structural reversibility during cycling. Benefiting from this coupled structural–electronic reconstruction, regenerated LFP delivers 146.9 mAh g⁻¹ at 1 C and retains 97.4% capacity after 500 cycles, outperforming the spent material. This work proposes a green and low-energy direct regeneration route for cathode materials and provides important theoretical guidance for their efficient repair and regeneration.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2026
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IF 21.1
You Xu,Huizhen Wang,Jiabing Geng,Yufeng Yuan,Kai Deng,Hongjie Yu,Ziqiang Wang,Hongjing Wang,Liang Wang
Electrosynthesis of cyclohexanone oxime (CYCO) from nitrite (NO 2 ⁻) and cyclohexanone (CYC) offers a sustainable alternative for green CYCO production. However, precise control of the NO 2 ⁻ reduction pathway to generate *NH 2 OH intermediates while suppressing CYC/CYCO hydrogenation and hydrogen evolution reaction remains challenging. Here, an intermetallic PdInBi metallene ( i -PdInBiene) catalyst was designed for CYCO electrosynthesis, achieving nearly 100% yield and an optimal Faradaic efficiency of 40.73 ± 2.74%. Combination of in situ characterizations with theoretical calculations reveal that the asymmetric p-d-p orbital hybridization in atomic-scale ordered configuration could regulate the local electronic structure of active sites and induce short pathway-hydrogen spillover. The *H modulation over specific active sites favors the selective reduction of NO 2 ⁻ to *NH 2 OH, promotes its spontaneous coupling with CYC to produce CYCO, while suppressing undesired side reactions. The system’s economic feasibility and broad substrate applicability further highlight its potential as a mild, sustainable strategy for oxime electrosynthesis.
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ACS Sustainable Chemistry & Engineering
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2014
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IF 0
Rui-Ying Bao,Wei Yang,Xin-Feng Wei,Bang-Hu Xie,Ming-Bo Yang
Melt processing of polymers is preferable in industry. Unfortunately, for high molecular weight (Mw) poly(l-lactide)/poly(d-lactide) (PLLA/PDLA) blends, exclusive formation of stereocomplex (sc) crystallites from the melt has never been achieved. We proposed a new and simple approach to enhance the melt crystallization of sc crystallites from high Mw PLLA/PDLA by using poly(ethylene glycol) (PEG). The crystallizability of the PLLA/PDLA blend is greatly enhanced by PEG during crystallization from the melt. The resulted crystalline structures depend on the Mw and content of PEG, and the crystallinity of sc crystallites increases with increasing content or decreasing Mw of PEG. More importantly, exclusive formation of sc crystallites is achieved in the blends with 10% PEG having Mw values of 1000 or 2000 g mol–1. Polarized optical microscopy (POM) observation shows that the spherulitic growth rates of sc crystallites are accelerated. The results demonstrate that segmental mobility of polylactides (PLA) chains plays a dominant role on the formation of sc crystallites from melt and provide a simple way to prepare sc crystallites from high Mw PLA by melt blending and enlarge the applications of PLA.
研究领域:
高分子科学
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材料工程
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聚合物结晶
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ACS Applied Materials & Interfaces
·
2022
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IF 10.383
Haoqiang Wang,Yu Wang,Yani Liu,Shuming Dou,Wei Gan,Qunhui Yuan
To alleviate kinetics imbalance and capacity insufficiency simultaneously, a novel hierarchical structure (SnCu2Se4/d-Ti3C2Tx/NPC) composed of delaminated Ti3C2Tx, SnCu2Se4 nanoparticles, and N-doped porous carbon layers is designed as a battery-type anode for lithium/sodium ion hybrid capacitor (LIC/SIC). The combination of SnCu2Se4 nanoparticles with high specific capacity, d-Ti3C2Tx with accelerated ion diffusion path, and NPC with enhanced electronic conductivity makes the SnCu2Se4/d-Ti3C2Tx/NPC composite possess excellent cycling stabilities in half-cell lithium-ion and sodium-ion batteries (LIB and SIB), with capacities of 114 mAh g–1 after 6000 cycles at 10 A g–1 for LIB and 296 mAh g–1 after 900 cycles at 1.0 A g–1 for SIB. The rate performance is also outstanding, with recovered capacity of 738 mAh g–1 at 0.1 A g–1 after cycles at current densities up to 50 A g–1 for LIB. Subsequently, LIC and SIC based on the SnCu2Se4/d-Ti3C2Tx/NPC anode and activated carbon cathode exhibit high energy densities of 147.9 and 158.6 Wh kg–1 at a power density of 100 W kg–1, respectively. They also possess distinctive long lifespans with capacity retentions of 78 and 81% after 10,000 cycles at 1.0 A g–1, respectively, demonstrating the feasibility of SnCu2Se4/d-Ti3C2Tx/NPC toward energy devices requiring high energy density, power density, and long-term stability.
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