APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
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2021
·Vol. 193
·期 7
·2029-2042
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IF 2.926
Xu Jian-Miao,Cao Hui-Ting,Wang Ming,Ma Bao-Jian,Wang Liu-Yu,Zhang Kai,Cheng Feng,Xue Ya-Ping,Zheng Yu-Guo
d -amino acid oxidase (DAAO) is widely used in the industrial preparation of l -amino acids, and cultivating Escherichia coli ( E. coli ) expressing DAAO for the biosynthesis of l -phosphinothricin ( l -PPT) is very attractive. At present, the biomass production of DAAO by fermentation is still limited in large-scale industrial applications because the expression of DAAO during the fermentation process inhibits the growth of host cells, which limits higher cell density. In this study, the factors that inhibit the growth of bacterial cells during a 5-L fed-batch fermentation process were explored, and the fermentation process was optimized by co-expressing catalase (CAT), by balancing the biomass and the enzyme activity, and by adding exogenous d -alanine ( d -Ala) to relieve the limitation of DAAO on the cells and optimize fermentation. Under optimal conditions, the DO-STAT feeding mode with DO controlled at 30% ± 5% and the addition of 27.5 g/L lactose mixed with 2 g/L d -Ala during induction at 28 °C resulted in the production of 26.03 g dry cell weight (DCW)/L biomass and 390.0 U/g DCW specific activity of DAAO; an increase of 78% and 84%, respectively, compared with the initial fermentation conditions. The fermentation strategy was successfully scale-up to a 5000-L fermenter.
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DYES AND PIGMENTS
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2025
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IF 4.2
Zewei Song,Shuang Li,Yijie Zou,Yinghan Li,Xun Sun,Shouzhi Pu,Zhao Chen
Three D-A-A′ architecture isomeric luminogens ( o -, m -, p -DMAc-Ph 2 CO-BT ) were designed and synthesized, comprising acridine (donor, D), benzophenone (acceptor, A), and trifluoromethylphenol (acceptor, A′) units. Impressively, all three prepared compounds achieved concentration-dependent high-quality white-light emission, which resulted from the integration of bluish-violet single molecules and yellow excimers. Furthermore, solid p -DMAc-Ph 2 CO-BT exhibited reversible mechanofluorochromism (MFC), with a high-contrast fluorescence shift from blue-green to yellow-green upon grinding, rendering it suitable for a rewritable medium. Single-crystal structural analysis revealed the underlying mechanism of force-responsiveness among the isomers that only o -DMAc-Ph 2 CO-BT possessed C–H⋯F interactions, accompanied by diverse and shorter intermolecular contacts, leading to rigid stacking and MFC passivity. In contrast, p -DMAc-Ph 2 CO-BT showed the weakest intermolecular interactions and the most loosely packed structure, endowing it with high sensitivity to mechanical force. This study demonstrated the critical role of intermolecular interactions in modulating the MFC, providing valuable insights for the rational design of intelligent fluorescent materials.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2025
·
IF 9.7
Xinzhi Wang,Jing Jiang,Jiayi Zhang,Qiyao Zeng,Aike Liu,Lunhong Ai
Defect engineering is considered one of the most powerful strategies for regulating the catalytic activity of electrocatalysts. A deep understanding of the defect-involved mechanism in electrocatalytic process is of great importance but remains a challenging task. In this study, an anionic Se-vacancy (V Se ) was introduced into iron diselenide (FeSe 2 ) nanoarrays, enabling the catalyst to exhibit improved electrocatalytic performance for sulfion oxidation reaction (SOR). The Se vacancy incorporated can modulate surface electronic structure and optimize the proximity of the d-p band center, strengthening the interaction between FeSe 2 -V Se and S atom. This endows the FeSe 2 /IF-V Se catalyst with the excellent electrocatalytic activity for SOR. As an alternative reaction to oxygen evolution reaction (OER), the low-potential SOR enables an ultralow electricity consumption in SOR-hydrid seawater electrolysis, avoiding detrimental chlorine electrochemistry. A proof-of-concept self-powered system based on FeSe 2 -V Se electrodes was constructed by integrating Zn-nitrite battery and SOR-hybrid seawater electrolyzer to produce high-value hydrogen, sulfur and ammonia. This work offers a fascinating strategy for the construction of an advanced energy system to produce hydrogen while utilizing seawater and wastewater.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2024
·
IF 20.2
Yuan Qin,Zihao Ou,Chaozhong Guo,Yao Liu,Rong Jin,Chuanlan Xu,Haifeng Chen,Yujun Si,Honglin Li
Regulating the electronic structure by phosphor-doping is a preferred strategy to boost the performance of carbon-based catalysts for oxygen reduction reaction (ORR). Here, a porous Fe, P, N-codoped carbon catalyst (PCF-FeTz-900) is designed by a phytic acid-assisted thermal etching strategy, in which P atoms are first doped into the carbon matrix to form a stable P C bond, and then FeN 4 sites are produced from Fe-2,4,6-Tris(2-pyridyl)-s-triazine complex (Fe-TPTz). Theoretical calculations suggest that the electrons are transferred from the doped P atom to the neighboring FeN 4 sites, which facilitates the ORR at the Fe sites by reducing the energy barrier and the adsorption energy of intermediates. Additionally, the P-doped FeN 4 (FeN 4 P) structure manifests a lower free energy difference than that of FeN 4 and the d -band center of Fe is also lowered, which further ensures its higher ORR catalytic ability. As a result, the PCF-FeTz-900 catalyst exhibits superior ORR activity and stability in alkaline electrolyte, and the assembled primary zinc-air battery shows greater performances compared to the commercial Pt/C catalyst. This work can provide an effective pathway for modulating the performance of doped-carbon materials in energy conversion devices.
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ADVANCED MATERIALS
·
2025
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IF 26.8
Rubing Bai,Peihao Huo,Nanlong Zheng,Yujia Li,Wenchao Yan,Peiyu Fang,Xiaoyu Zhou,Zuqiang Bian,Zhiwei Liu
Light-emitting electrochemical cells (LECs) have good prospects in the solid-state lighting field due to their simple single-layer structure and low manufacturing cost. However, the lack of high-efficiency blue LECs limits their development and further application. To solve this problem, many luminescent materials with various mechanisms such as fluorescence, phosphorescence, and thermally-activated delayed fluorescence are studied as emitters in LECs. Besides these materials, herein lanthanide europium(II) (Eu(II)) complex with d-f transition is demonstrated as a new type of emitter for LECs. In detail, a blue emitting Eu(II) complex bis[hydrotris(3-tert-butylpyrazolyl)borate]europium(II) is dispersed into 9-(3-(triphenylsilyl)phenyl)-9H-3,9′-bicarbazole host and tetrahexylammonium tetrafluoroborate electrolyte to make a blend film, which attains a high photoluminescence quantum yield of ≈100%. The LEC using the blend film as an active layer achieves a blue emission with Commission Internationale de L'Eclairage coordinates of (0.12, 0.18) and a record-breaking external quantum efficiency of 19.8%, corresponding to an exciton utilization efficiency of ≈100%. This work reveals that Eu(II) complex with d-f transition is a promising emitter toward high-performance blue LECs, which can inspire further research on Eu(II) complex based LECs.
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Food Bioscience
·
2026
·
IF 6.2
Yucheng Li,Bo Fan,Yu-Cai He
E. coli P8 expressing a glycosyltransferase from Panax ginseng was constructed. • Coupling glycosyltransferase and sucrose synthase (SS) was adopted to yield Reb D. • Regeneration of UDPG in situ from UDP and sucrose was used to convert Reb A to Reb D. • DES (ChCl:glycerol=1:2) –H 2 O (4:6, v/v) enhances the conversion of Reb A to Reb D. • P8 cell and SS could convert 19.3 g/L Reb A to Reb D (21.0 g/L) in 8 h. Rebaudioside A (Reb A) can be converted into Rebaudioside D (Reb D) through a glycosylation reaction. Reb D is a higher-value sweetener with a very low caloric value, a glycemic index of zero, and greater sweetness than Reb A. However, its naturally low abundance limits large-scale production. Accordingly, this study developed an efficient bioconversion system for Reb D production. Two recombinant Escherichia coli strains were constructed and designated E. coli P8 and E. coli SS. E. coli P8 expressed a glycosyltransferase derived from Panax ginseng , whereas E. coli SS expressed a sucrose synthase derived from Arabidopsis thaliana . In the coupled system, sucrose synthase used sucrose and UDP to regenerate UDP-glucose (UDPG) in situ, while the glycosyltransferase catalyzed the conversion of Reb A into Reb D. A deep eutectic solvent (DES) composed of choline chloride and glycerol at a ChCl:Gly molar ratio of 1:2 was mixed with water to form a DES–H 2 O reaction medium. Under the selected conditions of pH 7.5, 45 °C, and a DES-to-water volume ratio of 40:60, P8 whole cells and an SS crude lysate converted 19.3 g/L Reb A into 21.0 g/L Reb D within 8 h using 500 mmol/L sucrose and 10 mmol/L UDP as co-substrates, corresponding to a molar yield of 93%. This study demonstrates an efficient and potentially environmentally friendly strategy for producing Reb D and other steviol glycosides. Download: Download high-res image (402KB) Download: Download full-size image
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SEPARATION AND PURIFICATION TECHNOLOGY
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2026
·
IF 9.1
Zhaoguo Zhang,Dan Mei,Noor Ul Afsar,Yanan Chen,Jin Tang,Ke Liu,Jawad Ali,Dong Wang
Advanced membrane design with high proton selectivity is essential in industry for effective acid recovery and the elimination of multivalent metal ions, thereby reducing chemical consumption and environmental impact. Here, we present Vinyl carbazole (Vc)-functionalized membranes with adjustable interchain spacing (d-spacing) and hydrophilic channels to enhance acid recovery via diffusion dialysis. Adjusting Vc content optimizes the membrane structure for balanced proton conduction and metal-ion transport rejection. Moderate Vc incorporation (2–8 mol%) expands interchain spacing and simultaneously facilitates in situ crosslinking, thereby forming dense networks that suppress water uptake. This form of interconnected ionic channels significantly increases U acid flux: U acid values are 18.21 × 10 −3 m h −1 (HCl/FeCl 2 ) and 14.02 × 10 −3 m h −1 (H 2 SO 4 /FeSO 4 ), while metal ion transport (U salt ) is suppressed to 2.85 × 10 −6 m h −1 (HCl/FeCl 2 ) and 4.5 × 10 −6 m h −1 (H 2 SO 4 /FeSO 4 ). In the HCl/FeCl 2 system, the membrane achieved high selectivity (S ≈ 997.81–4515.99). However, further increasing the Vc content up to 10 mol% (PT 0.9 -Vc 0.1 ) enhances crosslinking, which reduces selectivity (S ≈ 1612.59) and proton flux (U acid = 9.20 × 10 −3 , and 8.86 × 10 −3 m h −1 for HCl/FeCl 2 and H 2 SO 4 /FeSO 4 ). These findings suggest that moderate Vc levels optimize high H + conductivity and prevent metal-ion crossover through steric effects, Donnan exclusion, and charge-assisted hopping via the carbazole nitrogen, thereby controlling Fe 2+ crossover. The adjustable microstructure also minimizes swelling, creating durable membranes suitable for industry use.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2026
·
IF 9.6
Shixuan Yu,Shiying Fan,Shaomin Liu,Xinyong Li
The direct photocatalytic oxidation of inert methane (CH 4 ) to high-value-added oxygenates remains a significant challenge. This study precisely controls generated reactive oxygen species (ROS) types by modulating interfacial electronic interactions in Zn-Pt δ− -PS photocatalysts, promoting CH 4 conversion into formaldehyde (HCHO). Under ambient conditions, a HCHO production rate reached 3308 μmol·g −1 with a high selectivity of 77%. In situ spectroscopy and theoretical studies demonstrated that the elevated Pt d-band center in Pt-*OOH (* represents the adsorption state) lowered the occupancy of antibonding orbitals, thereby enhancing the interaction between Pt and *OOH, markedly polarizing the O O bond, facilitating its cleavage, and enabling the preferential in situ generation of •OH rather than •OOH. Furthermore, adequate •OH radical accelerated activation of the C H bond in CH 4 and induced the cleavage of its O H bond in methanol (CH 3 OH), promoting HCHO formation. This strategy may guide the rational design of the catalyst's electronic structure to enhance CH 4 photocatalytic oxidation. Download: Download high-res image (175KB) Download: Download full-size image
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JOURNAL OF CHROMATOGRAPHY A
·
2025
·
IF 4
Yan Zhang,Yaqian Zhou,Ping Liu,Yufeng Zhang,Yiqun Wan,Fan Liu,Genlin Sun,Lihua Chen,Jun Wang
A rapid and efficient method is highly desired for the determination of phenylurea herbicides (PUHs) in edible and medicinal herbs. Herein, we report a dispersive solid-phase extraction (d-SPE) method using a metal-organic framework Zn 4 O(NTB) 2 as the adsorbent. The microporous Zn 4 O(NTB) 2 can selectively extract targets while excluding matrix substances. Under optimized conditions, this method exhibits high sensitivity, with a limit of detections (LODs) and limits of quantification (LOQs) in the range of 0.09–0.32 ng mL -1 and 0.32–1.08 ng mL -1 , respectively. Moreover, the recoveries reach the range of 69.8 %-83.3 % with relative standard deviations below 6.2 %. The optimized adsorption efficiency of PUHs can be rapidly achieved in a short time of 10 min. Additionally, the adsorbent can be facilely regenerated with methanol washing. Furthermore, computational simulations reveal the guest-host interactions involving hydrogen bonding, π-π stacking interactions, and Van der Waals interactions. Practically, the established method has been successfully validated for extracting PUHs from Dendrobium candidum and Ganoderma lucidum samples.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2025
·
IF 6.2
Jing Gao,Hao Dong,Peichao Zhang,Kunpeng Gao,Haipeng Su,Jianan Sun,Xiangzhao Mao
Whole-enzyme cascade degradation of crystalline α-chitin is a long-term pursuit, which has been hampered by low transformation efficiency and heterogeneous products. Here, a novel β-N-acetylglucosaminidase (NAGase) from Sulfuriroseicoccus oceanibius, designated Sohex, was biochemically characterized. The enzyme demonstrated high hydrolytic activity toward N-acetyl chitooligomers (N-acetyl COSs) and exhibited promotive effects on crystalline α-chitin degradation. Furthermore, a whole-enzyme cascade degradation system based on lytic polysaccharide monooxygenases (LPMOs), chitinases, and Sohex was developed for N-acetyl-D-glucosamine (GlcNAc) production. This system achieved a maximum conversion rate of 45.93%, with GlcNAc purity reaching 99.99%. The addition of Sohex significantly enhanced α-chitin degradation efficiency, achieving conversion rates of 1.37-fold and 2.46-fold compared to the LPMOs + chitinases system and the sole chitinases system, respectively. Moreover, the promotion mechanisms of Sohex in conjunction with LPMOs and chitinases were elucidated, making the cascade system a promising strategy for converting crystalline α-chitin into high-purity GlcNAc.
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GREEN CHEMISTRY
·
2026
·
IF 9.2
zonghao xu,Zongyu Guan,Yan Song,Xiran Zhao,Yaodong Yuan,Zhipeng Yan,Junhang Tian,Xueyi Sun,Biwei Xiao,Weidong Zhuang
Direct recycling of spent LiFePO₄ (LFP) cathodes is a sustainable and energy-efficient strategy for recovering valuable materials while preserving the intrinsic olivine structure. Herein, we report a LiI-assisted regeneration approach that achieves electronic structure reconstruction and reinforced Fe–O d–p orbital hybridization, enabling simultaneous repair of structural defects and enhancement of Li⁺ transport kinetics. The reductive I⁻ ions promote Fe³⁺→Fe²⁺ conversion and effectively eliminate Fe–Li antisite defects, while Li⁺ replenishment restores the stoichiometry of degraded LFP. Density functional theory (DFT) calculations reveal that LiI triggers a downward shift of the Fe 3d band center and an upward shift of the O 2p band center, narrowing their energy separation and strengthening Fe–O covalency. These electronic modifications reduce Li⁺ migration barriers and reconstruct continuous Li⁺ transport channels, as further validated by ab initio molecular dynamics simulations. In-situ XRD measurements confirm reduced unit-cell volume fluctuations and enhanced FeO₆–PO₄ framework stability, demonstrating improved structural reversibility during cycling. Benefiting from this coupled structural–electronic reconstruction, regenerated LFP delivers 146.9 mAh g⁻¹ at 1 C and retains 97.4% capacity after 500 cycles, outperforming the spent material. This work proposes a green and low-energy direct regeneration route for cathode materials and provides important theoretical guidance for their efficient repair and regeneration.
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APPLIED CATALYSIS B-ENVIRONMENTAL
·
2026
·
IF 21.1
You Xu,Huizhen Wang,Jiabing Geng,Yufeng Yuan,Kai Deng,Hongjie Yu,Ziqiang Wang,Hongjing Wang,Liang Wang
Electrosynthesis of cyclohexanone oxime (CYCO) from nitrite (NO 2 ⁻) and cyclohexanone (CYC) offers a sustainable alternative for green CYCO production. However, precise control of the NO 2 ⁻ reduction pathway to generate *NH 2 OH intermediates while suppressing CYC/CYCO hydrogenation and hydrogen evolution reaction remains challenging. Here, an intermetallic PdInBi metallene ( i -PdInBiene) catalyst was designed for CYCO electrosynthesis, achieving nearly 100% yield and an optimal Faradaic efficiency of 40.73 ± 2.74%. Combination of in situ characterizations with theoretical calculations reveal that the asymmetric p-d-p orbital hybridization in atomic-scale ordered configuration could regulate the local electronic structure of active sites and induce short pathway-hydrogen spillover. The *H modulation over specific active sites favors the selective reduction of NO 2 ⁻ to *NH 2 OH, promotes its spontaneous coupling with CYC to produce CYCO, while suppressing undesired side reactions. The system’s economic feasibility and broad substrate applicability further highlight its potential as a mild, sustainable strategy for oxime electrosynthesis.
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