Journal of Sulfur Chemistry
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2018
·
IF 1.32
Yanmei Chen,Xuefang Shang,Xing Zhao,Jie Li,Jianmei Yuan,Hongli Chen,Jinlian Zhang,Tianyun Wang
Two probes based on novel Copper(II) complexes were developed in order to obtain H2S molecular probes with higher selectivity. The molecular structures of the complexes were characterized by 1H NMR, HRMS, IR and elemental analysis. The interaction of the compounds with biologically important anions and amino acids was determined by UV-vis and Fluorescence titration experiments. Results indicated that the compounds showed the highest binding ability for HS− among studied anions (AcO−, H2PO4−, F−, Cl−, Br− and I−) and amino acids (GSH, HCys and Cys) accompanied by blue shift phenomenon in pure DMSO and aqueous solution. The possible mechanism of host–guest interaction may be that the Copper(II) ion of complex was captured by HS− and free ligand released which showed a remarkable changes in UV-vis absorption. In addition, cytotoxicity of the synthesized compounds was studied on MCF-7 cells. Results indicated that the synthesized compounds had low cytotoxicity over a concentration range of 0–150 µg⋅mL−1, which exhibited that the synthesized probes could be used to detect H2S in vivo.The probes based on novel copper(II) complexes were synthesized and obtained by the reaction of substituent salicylaldehyde with amine derivatives, and then reacted with Cu(CH3COO)2·H2O, respectively. Investigation on the interference from other species suggested that copper(II) complexes have high selectivity for HS− over other anions (AcO−, , F−, Cl−, Br− and I−), followed by the release of the copper ion to give a remarkable increase in UV–VIS absorption in pure DMSO solution and aqueous solution.
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NEW JOURNAL OF CHEMISTRY
·
2018
·Vol. 42
·期 4
·3097-3101
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IF 3.201
Qiaoling Liu,Nan Zhang,Haoying Shi,Wenyan Ji,Xiaoqian Guo,Wen Yuan,Qin Hu
Highly fluorescent carbon dots (CDs) are prepared by a one-step microwave heating method using citric acid, L-cysteine and dextrin as the precursors. The average size of the CDs is 2.61 nm with good water dispersibility and photostability. Compared with undoped CDs, small amounts of nitrogen (N) and sulfur atom (S) co-doped CDs exhibit a red shift of the absorption spectra to the visible region and lead to a high quantum yield of 22%. Due to the large amount of carboxyl and hydroxyl groups (−COOH and −OH) on the surface of the CDs, Cu2+ is selectively detected in aqueous solution at pH 7.0 with a detection limit as low as 20 nM, which is much lower than the maximum concentration of Cu2+ in drinking water (∼20 μM) permitted by the US Environmental Protection Agency (EPA). Moreover, the application in tap water samples further demonstrates that the as-synthesized CDs are good candidates for the monitoring of Cu2+ in environmental water samples.
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MICROCHIMICA ACTA
·
2018
·Vol. 185
·期 10
·1-10
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IF 5.705
Huang Yan-Qin,Yang Li-Na,Wang Yong-Sheng,Xue Jin-Hua,Chen Si-Han
The authors report on a one-pot approach for synthesizing highly fluorescent protamine-stabilized gold nanoclusters. These are shown to be a viable nanoprobe for selective and sensitive fluorometric determination of lead(II) via quenching of fluorescence via Pb(II)-Au(I) interaction. Under optimized conditions, fluorescence measured at excitation/emission peaks of 300/599 nm drops in the 80 nM–15 μM lead(II) concentration range. The detection limit is 24 nM, and relative standard deviations (for n = 11) at concentrations of 0.10, 4.0 and 15 μM are 1.6, 2.5 and 1.9%, respectively. The relative recoveries of added lead(II) in the water samples ranged from 97.9 ± 2.29% to 101.2 ± 1.83%.
Lead(II) ions are found to be able to selectively and sensitively quench the fluorescence of the protamine-gold nanoclusters (PRT-AuNCs). Thereby, an inexpensive, selective and sensitive lead(II) assay was established.
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DRUG DELIVERY
·
2018
·
IF 3.095
Liuxiang Chu,Aiping Wang,Ling Ni,Xiuju Yan,Yina Song,Mingyu Zhao,Kaoxiang Sun,Hongjie Mu,Sha Liu,Zimei Wu,Chunyan Zhang
Glioblastoma is the most common malignant brain tumor. Efficient delivery of drugs targeting glioblastomas remains a challenge. Ephrin type-A receptor 3 (EPHA3) tyrosine kinase antibody-modified polylactide-co-glycolide (PLGA) nanoparticles (NPs) were developed to target glioblastoma via nose-to-brain delivery. Anti-EPHA3-modified, TBE-loaded NPs were prepared using an emulsion-solvent evaporation method, showed a sustained in vitro release profile up to 48 h and a mean particle size of 145.9 ± 8.7 nm. The cellular uptake of anti-EPHA3-modified NPs by C6 cells was significantly enhanced compared to that of nontargeting NPs (p < .01). In vivo imaging and distribution studies on the glioma-bearing rats showed that anti-EPHA3-modified NPs exhibited high fluorescence intensity in the brain and effectively accumulated to glioma tissues, indicating the targeting effect of anti-EPHA3. Glioma-bearing rats treated with anti-EPHA3-modified NPs resulted in significantly higher tumor cell apoptosis (p < .01) than that observed with other formulations and prolonged the median survival time of glioma-bearing rats to 26 days, which was 1.37-fold longer than that of PLGA NPs. The above results indicated that anti-EPHA3-modified NPs may potentially serve as a nose-to-brain drug carrier for the treatment of glioblastoma.
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Photodiagnosis and Photodynamic Therapy
·
2019
·
IF 2.589
Asif Jan,Chengcheng Liu,Hong Deng,Jiao Li,Wenpeng Ma,Xiaoyan Zeng,Yanhong Ji
Background and aim The extensive use of antifungal drugs has led to resistance from Candida albicans . The search for alternative treatment against drug-resistant C. albicans is highly desirable. Antimicrobial photodynamic therapy (aPDT) is an emerging and promising approach for treating localized and superficial C. albicans infections. The aim of this study was to investigate the photodynamic inactivation (PDI) effects of hypocrellin B (HB) on azole-sensitive and resistant C. albicans in vitro . Methods The PDI efficacies of HB on standard C. albicans strain (ATCC 10231), azole-sensitive clinical isolate of C. albicans , and azole-resistant clinical isolate of C. albicans were assessed. The uptake of HB in C. albicans cells was investigated by confocal laser scanning microscopy (CLSM). The PDI effects on cellular structure and surface characteristics were investigated by transmission electron microscopy (TEM) and scanning electron microscopy (SEM). Results HB exhibited no significant dark toxicity, but inactivated the azole-sensitive and resistant C. albicans in a light-dose and PS concentration-dependent manner. CLSM images indicated that PDI treated C. albicans cells showed stronger fluorescence compared to untreated cells. TEM images suggested that significant damage to the cell wall, membrane, and cytoplasm were induced by HB-mediated PDI. SEM analysis revealed that the surface of C. albicans cells became twisted and ruptured after PDI treatment. Conclusions Azole-sensitive and resistant C. albicans could be effectively inactivated by HB in the presence of light, and HB-mediated aPDT shows promise as an antifungal treatment for C. albicans .
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ANALYTICA CHIMICA ACTA
·
2017
·
IF 4.95
Caihong Wang,Jie Gao,Yili Cao,Hongliang Tan
Nanozymes have been extensively exploited in the construction of colorimetric sensors in the past decade. However, as lack of specific recognition element, these sensors are mostly limited in the detection of hydrogen peroxide and its related agents. In this work, a colorimetric assay for the detection of alkaline phosphatase (ALP) activity was developed by employing copper (II)-based metal-organic frameworks (Cu-MOFs) as peroxidase mimic and pyrophosphate (PPi) as recognition element. Benefiting from its intrinsic porosity, Cu-MOFs built from one-step self-assembly showed a superior catalytic activity over horseradish peroxidase. It was found that, in the presence of PPi, the catalytic activity of Cu-MOFs was greatly inhibited due to the ultrastrong binding ability of Cu 2+ with PPi, and no colorimetric signal was recorded. However, after incubation with ALP, such negative behavior of PPi can be suppressed as the hydrolysis of PPi into orthophosphates (Pi) with low affinity to Cu 2+ , producing a colorimetric signal depending on ALP level. On this basis, a way for quantitative analysis of ALP activity was paved accordingly. Compared with previously reported ALP assays based on nanomaterials , this assay is simple, cost-effective and visualized, which not only allows highly sensitive analysis of ALP activity with a detection limit of 0.19 U/L, but also exhibits a good sensing performance in serum samples. Furthermore, based on the distinct colorimetric signals induced by PPi and ALP, an IMPLICATION logic gate was constructed. We hope that this study could provide a new insight for designing nanozyme-based highly specific colorimetric sensors.
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RSC Advances
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2017
·Vol. 7
·期 80
·50420-50424
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IF 3.108
Minhao Xie,Kai Zhang,Feifan Zhu,Hao Wu,Pei Zou
Herein, a simple, highly sensitive and selective exonuclease III (Exo III)-based sensor was developed for mercury ions Hg2+ detection without the need of complicated, high-cost and time-consuming labeling. In this strategy, two oligonucleotides were employed for the experiment design. DNA1 was prepared with one DNA strand which was designed as a quadruplex-forming oligomer, and which was constructed as 3′-protruding to ensure the probe cannot be digested by Exo III. When the target Hg2+ is introduced into the sensing system, it associates with DNA1 and DNA2 to form double strand DNAs (DNA1/DNA2 duplexes) which have a blunt 3′-terminus. Exo III then can degrade part of DNA1, releasing the DNA1 and Hg2+, and ultimately liberating the G-rich oligomer. This released G-rich oligomer folds into a G-quadruplex structure and thus allows the formation of DNAzyme in the presence of hemin. The formed DNAzyme can effectively catalyze the H2O2-mediated oxidation of ABTS, giving rise to a change in solution color. The released DNA1 and Hg2+ are free to bind to another 3′-protruding terminus of DNA1 to trigger a new digestion reaction, leading to significant amplification of the signal. The present new strategy shows a limit of detection as low as 66 pM and excellent selectivity toward Hg2+ over a wide range of metal ions.
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LUMINESCENCE
·
2017
·Vol. 33
·期 1
·161-167
·
IF 1.509
Lili Chen,Hongliang Tan,Fugang Xu,Li Wang
With recognition of the biological importance of hydrogen sulfide (H 2 S), we present a simple and effective fluorescent probe for H 2 S using a Tb 3+ coordination polymer–Cu 2+ compound (DPA/Tb/G–Cu 2+ ). Dipicolinic acid (DPA) and guanosine (G) can coordinate with Tb 3+ to form a macromolecular coordination polymer (DPA/Tb/G). DPA/Tb/G specifically binds to Cu 2+ in the presence of coexisting cations, and obvious fluorescence quenching is observed. The quenched fluorescence can be exclusively recovered upon the addition of sulfide, which is measured in the mode of time-resolved fluorescence. The fluorescence intensities of the DPA/Tb/G–Cu 2+ compound enhance linearly with increasing sulfide concentrations from 1 to 30 μM. The detection limit for sulfide in aqueous solution is estimated to be 0.3 μM (at 3σ). The DPA/Tb/G–Cu 2+ compound was successfully applied to sense H 2 S in human serum samples and exhibited a satisfactory result. It displays some desirable properties, such as fast detection procedure, high selectivity and excellent sensitivity. This method is very promising to be utilized for practical detection of H 2 S in biological and environmental samples.
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Catalysts
·
2017
·Vol. 7
·期 11
·327
·
IF 3.082
Hao Liang,Shanshan Sun,Yan Zhou,Yanhui Liu
In this study, a one-step and facile immobilization of enzymes by self-assembly of zinc ions and adenine in aqueous solution with mild conditions was reported. Enzymes, such as glucose oxidase (GOx) and horseradish peroxidase (HRP), could be efficiently encapsulated in Zn/adenine coordination polymers (CPs) with high loading capacity over 90%. When the enzyme was immobilized by CPs, it displayed high catalytic efficiency, high selectivity and enhanced stability due to the protecting effect of the rigid framework. As a result, the relative activity of Zn/adenine nano-CP-immobilized GOx increased by 1.5-fold at pH 3 and 4-fold at 70 to 90 °C, compared to free GOx. The immobilized GOx had excellent reusability (more than 90% relative activity after being reused eight times). Furthermore, the use of this system as a glucose biosensor was also demonstrated by co-immobilization of two enzymes, detecting glucose down to 1.84 µM with excellent selectivity. The above work indicated that in-situ self-assembly of Zn/adenine CPs could be a simple and efficient method for biocatalyst immobilization.Keywords:adenine;enzyme;self-assembly;coordination polymers;immobilization
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Journal of Materials Chemistry B
·
2017
·Vol. 5
·期 37
·7692-7700
·
IF 4.543
Jie Gao,Caihong Wang,Hongliang Tan
This work demonstrated the potential of lanthanide/nucleotide coordination polymers as a host platform for encapsulating enzymes and fluorescent nanoparticles to fabricate multifunctional composites. The self-adaptive assembly of terbium ions (Tb3+), adenosine monophosphate (AMP), glucose oxidase (GOx), and carbon dots (CDs) leads to the formation of a GOx&CDs@AMP/Tb composite with catalytic and fluorescence properties. Owing to the confinement effect, the GOx&CDs@AMP/Tb composite exhibited about 2-fold higher catalytic activity than free GOx. Meanwhile, significantly enhanced storage stability and negligible leakage of GOx were also achieved. On this basis, by considering the advantages of carboxyphenylboronic acid (CPBA) in the sensitization of Tb3+ fluorescence and specific recognition of hydrogen peroxide, a dual-emissive GOx&CDs@AMP/Tb-CPBA composite was further fabricated for the ratiometric sensing of glucose. Benefiting from the synergistic effect of GOx and CDs in the highly efficient transfer of intermediates and the elimination of environmental fluctuations, the presented ratiometric sensor not only displayed an ultrahigh sensitivity with a detection limit of 80 nM, but also allowed glucose to be easily identified by the naked eye under a UV lamp. We hope that this study will provide a new insight into the design and application of coordination polymer-based multifunctional composites.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2024
·Vol. 22
·期 1
·1-15
·
IF 10.6
Li Jingchao,Wang Tingting,Shi Yuanfei,Ye Zichen,Zhang Xun,Ming Jiang,Zhang Yafei,Hu Xinyan,Li Yun,Zhang Dongsheng,Xu Qianhe,Yang Jun,Chen Xiaolan,Liu Nian,Su Xinhui
Radionuclides internal radiotherapy (RIT) is a clinically powerful method for cancer treatment, but still poses unsatisfactory therapeutic outcomes due to the hypoxic characteristic of tumor microenvironment (TME). Catalase (CAT) or CAT-like nanomaterials can be used to enzymatically decompose TME endogenous H 2 O 2 to boost TME oxygenation and thus alleviate the hypoxic level within tumors, but their effectiveness is still hindered by the short-lasting of hypoxia relief owing to their poor stability or degradability, thereby failing to match the long therapeutic duration of RIT. Herein, we proposed an innovative strategy of using facet-dependent CAT-like Pd-based two-dimensional (2D) nanoplatforms to continuously enhance RIT. Specifically, rationally designed 2D Pd@Au nanosheets (NSs) enable consistent enzymatic conversion of endogenous H 2 O 2 into O 2 to overcome hypoxia-induced RIT resistance. Furthermore, partially coated Au layer afford NIR-II responsiveness and moderate photothermal treatment that augmenting their enzymatic functionality. This approach with dual-effect paves the way for reshaping TME and consequently facilitating the brachytherapy ablation of cancer. Our work offers a significant advancement in the integration of catalytic nanomedicine and nuclear medicine, with the overarching goal of amplifying the clinical benefits of RIT-treated patients. Graphical abstract
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Food Bioscience
·
2024
·
IF 4.8
Qiang Wang,Feng Zhang,Yuhong Zhang,Yu Zhang,Hongwei Wang,Jiajia Song,Huayi Suo
This study investigated the antifungal effect, mechanism, and potential application in yogurt of Lactiplantibacillus plantarum ( L. plantarum ) LPP95 isolated from Chinese pickles. The L. plantarum LPP95 showed a high inhibitory effect on the growth of five molds , with the strongest inhibitory effect observed on Penicillium sp. The cell-free supernatants (CFS) of L. plantarum LPP95 resulted in the shrinking of Penicillium sp. spores , and destroyed the integrity of the cell wall and membrane of spores, causing the leakage of nucleic acid from cells. The antifungal activity of CFS was not decreased after heat or proteolytic enzyme treatment but was affected by pH change. The inhibitory activity of CFS was associated with organic acids, and 66 organic acids were identified by LC-MS analysis. In addition, the validation experiments showed that trans -cinnamic acid, citric acid , trans -3-indoleacrylic acid, and DL-4-hydroxyphenyllactic acid all exhibited antifungal activity. Moreover, L. plantarum LPP95 effectively inhibited the growth of Penicillium sp. in yogurt. Overall, L. plantarum LPP95 and its CFS have the potential as biological preservatives.
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