FUEL
·
2025
·
IF 7.5
Xin Zhong,Baihong Li,Chao Li,Lijun Zhang,Shu Zhang,Xun Hu
As an indispensable fraction of majority biomass, inorganics might not be inert in activation, due to their potential interaction with volatiles/activators and steric hindrance created for accessing organics in inner structure by activator. This was verified herein by conducting the activation of rice husk (RH), with inherent silica leached partially or completely by KOH, with ZnCl 2 or K 2 C 2 O 4 as an activator at 550 or 800 °C. The results indicated that removal of SiO 2 promoted condensation reactions with ZnCl 2 while cracking reactions with K 2 C 2 O 4 , forming more activated carbon (AC) with ZnCl 2 (41.1 %) but less AC-K 2 C 2 O 4 on organic basis (26.8 %). This resulted from enhanced permeability and more sufficient contact of the activator to organics via eliminating steric hindrance. After removing SiO 2 , the specific surface area increased from 1348.8 to 1712.3 m 2 /g −1 with ZnCl 2 as an activator, while that with K 2 C 2 O 4 increased from 615.4 to 1121.6 m 2 /g −1 . Moreover, the degree of aromatization in activation also increased after removing SiO 2 , forming rigid aromatic skeleton preserving micropores formed (97.6 % versus 86.0 % with AC-RH-K 2 C 2 O 4 ). The in-situ IR characterization of activation process confirmed negative impacts of SiO 2 on aromatization process from segregation of organics, which further deterred development of pores.
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Cell Death Discovery
·
2025
·Vol. 11
·期 1
·1-16
·
IF 7
Liang Xue,Tian Ruofei,Li Ting,Wang Hao,Qin Yifei,Qian Meirui,Fan Jing,Wang Dan,Cui Hong-Yong,Jiang Jianli
Pancreatic cancer exhibits high mortality rates with limited therapeutic options. Emerging evidence suggests that the ketogenic diet may act as adjuvant therapy by triggering ferroptosis in cancer cells, though the underlying molecular mechanisms remain unclear. This study aims to investigate the molecular mechanisms linking ketogenic metabolism and ferroptosis, with an emphasis on key regulatory proteins. We demonstrated that pancreatic adenocarcinoma (PAAD) tissues significantly enhanced ketogenic and ferroptosis phenotypes compared to normal tissues, both correlating with poorer patient prognosis. These phenotypes showed strong interdependence mediated by CAV1. In the pancreatic tumor microenvironment, CAV1 was predominantly expressed in tumor cells. Through in vitro cell experiments, we clarified that Na-OHB downregulated CAV1 expression in pancreatic cancer cells, inhibiting the transcription of the CAV1/AMPK/NRF2 downstream ferroptosis-protective genes SLC7A11 and SLC40A1. Additionally, we demonstrated the interaction between CAV1 and SLC7A11 molecules; when CAV1 was downregulated, it affected the stability of SLC7A11, leading to the ubiquitination and degradation of the translated SLC7A11 protein. Through these dual mechanisms, Na-OHB caused Fe 2+ overload, lipid peroxidation accumulation, and oxidative stress in pancreatic cancer cells, ultimately triggering ferroptosis. In ketogenic diet-fed tumor-bearing mouse models, we also observed a significant increase in lipid peroxidation and other related biomarkers, while CAV1 and SLC7A11 levels were markedly decreased compared to the normal diet group. Our findings identify CAV1 as a pivotal molecular link between ketogenic metabolism and ferroptosis in pancreatic cancer. The multi-level regulatory axis involving CAV1-mediated transcriptional regulation and post-translational modifications provides mechanistic insights into ketogenic diet-induced ferroptosis, suggesting potential therapeutic targets for pancreatic cancer adjuvant treatment.
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Communications Biology
·
2025
·Vol. 8
·期 1
·1-15
·
IF 5.1
Fan Zhan,Xu Yilun,Lu Yafang,Li Xinlin,Xu Mengli,Liu Jinxin,Cai Zhenzhen,Liu Jiayang,Su Jingping,Wang Jialu,Luo Qingming,Zhang Zhihong,Liu Zheng
The excessive accumulation of neutrophils within the epidermis is a significant hallmark of cutaneous diseases; however, the mechanisms governing neutrophil transepidermal migration (NTEM) remain inadequately understood. In this study, we develop trichromatic-fluorescence-labeled chimeric mice by utilizing Cx3cr1 GFP/+ Lyz2 RFP/+ mice as bone marrow donors and Krt14 YFP/+ mice as recipients. This approach enables us to visualize the process of NTEM and the crosstalk between neutrophils and monocytes in a murine model of irritant contact dermatitis (ICD). Intravital imaging reveals a preferential transmigration of neutrophils through hair follicle (HF), where dermal neutrophils exhibit limited mobility and interact with dermal monocytes. Notably, 18 h following hapten exposure, dermal neutrophils continuously migrate toward HF regions and form clusters within 3 h. Importantly, MMP-9 is identified as essential for the NTEM process; the depletion of dermal monocytes results in a significant reduction of MMP-9 expression in the skin and inhibits the NTEM process in ICD. Mechanistically, dermal monocytes are found to be a crucial source of the cytokines TNF-α and CXCL2, which promote the upregulation of MMP-9 in neutrophils. Therefore, our results highlight HF regions as crucial gateways for dermal monocyte-modulated NTEM and provide visual insights into the crosstalk between neutrophils and monocytes in inflammatory skin disorders.
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高影响因子
ENVIRONMENTAL POLLUTION
·
2014
·
IF 3.902
Chao Li,Xin−Yi Cui,Ying−Ying Fan,Ying Teng,Zhong−Ren Nan,Lena Q. Ma
Physiologically based in vitro methods have been developed to measure bioaccessibility of organic contaminants in soils. However, bioaccessibility of hydrophobic organic contaminants (HOCs) can be underestimated by in vitro tests if gastrointestinal (GI) solution fails to provide sufficient sorption sink for HOCs. To circumvent this drawback, Tenax was included in GI solution as sorption sink to trap mobilized HOCs and maintain the desorption gradient between soil and GI solution. Polycyclic aromatic hydrocarbons (PAHs) were selected as target HOCs, and physiologically based extraction test (PBET) was selected as the in vitro method. Inclusion of Tenax in GI solution increased bioaccessibility of PAHs in five spiked soils from 8.25–20.8% to 55.7–65.9% and the bioaccessibility of PAHs in a field contaminated soil from 3.70–6.92% to 16.3–31.0%. Our results demonstrated the effectiveness of Tenax as sorption sink to enhance PAH mobilization in bioaccessibility measurement in soils.
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CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Yutong Wang,Sha Jiang,Jialiang Xie,Linlin Xie,Chuanliang Feng,Yongjie Wang,Guotao Xiang,Li Li,Yanhao Huang,Xianju Zhou,Feng Wang
Ratiometric luminescent thermometry is favored for its unique advantages and significant potential applications, particularly in the biomedical field. That has driven growing interest in the development of novel luminescent materials with ultra-high sensitivity in the physiological temperature range. Our study focuses on materials that exhibit temperature-induced phase transition within the physiological temperature range, thus facilitating the development of highly sensitive luminescent thermometers. Through a series of experimental explorations, we have discovered that LiYO 2 :1%Sm 3+ undergoes a phase transition from a monoclinic structure to a tetragonal structure within the temperature range of 320–330 K. Subsequently, a luminescent thermometer based on LiYO 2 :Sm 3+ phosphor was designed, capitalizing on the contrasting thermal response of the emissions associated with monoclinic and tetragonal structures, achieving ultra-high sensitivity of 17.51% K −1 at 322 K, making it suitable for the application in the biomedical field. Especially, the optimal temperature range for LiYO 2 : Sm 3+ thermometer aligns well with mitochondrial temperature, presenting potential applications in cancer diagnosis and photothermal therapy.
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CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Yixin Luo,Jingnan Yang,Fu Li,Xiaomei Zhou,Tongyan Yu,Qingxin Guan,Mo Qiu,Wei Li
The hydrogenation of glucose to sorbitol using Raney-Ni is a well-established process in industry, but it faces challenges such as high mannitol content, nickel leaching, and catalyst pyrophoricity. Here, Ni 6 Fe 1 /Al 2 O 3 nanoflower with high sorbitol selectivity and stability was synthesized from Ni x Fe y /Al-LDH precursors, where the distribution of iron in the alloy phase and support can be well-regulated. XANES and FT-EXAFS spectra verified the presence of Fe in the catalyst as NiFe alloy and FeO x . Multiple evidences unveiled that the NiFe alloy phase significantly improving the yield of sorbitol and the iron oxide species can regulate the acidity of the support, thus effectively reducing the byproduct mannitol. Delightfully, the optimal Ni 6 Fe 1 /Al 2 O 3 catalyst achieved a sorbitol yield of 99% and a mannitol yield of 0.45%, showing a significant advantage over Ni/Al 2 O 3 (77% and 1.3%) and Raney-Ni (57% and 1.3%) catalysts. Theoretical calculations indicate that the NiFe alloy surface is more conducive to glucose adsorption and sorbitol desorption, and the intrinsic high activity of NiFe alloy phase is due to the downshift of d-band center, which facilitates H* dissociation from the anti-bonding orbital. This study provides significant insights for designing catalysts with high activity and selectivity in converting lignocellulosic biomass.
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ENVIRONMENTAL POLLUTION
·
2025
·
IF 7.3
Xinyue Zhao,Siyu Liu,Lina Wang,Li Yang,Jun He,Chengjun Wang
A gas chromatography-flame ionization detection method was developed to investigate the occurrence and transformations of phthalate esters (PAEs) in atmospheric aqueous aerosol in Wuhan City. Six of PAEs were detected in collected aerosol samples and their concentrations in atmosphere were followed the order of diallyl phthalate (0.244 μg m −3 ) > diethyl phthalate (0.235 μg m −3 ) > di(2-ethylhexyl) phthalate (0.176 μg m −3 ) > dimethyl phthalate (0.110 μg m −3 ) > di-n-octyl phthalate (0.081 μg m −3 ) > dipropyl phthalate (0.024 μg m −3 ). The Redundancy analysis results indicated that, compared to humidity and wind speed, temperature had the most important influence on the occurrence of PAEs in aqueous aerosol. The hydrolysis of selected PAEs in aqueous extracts of aerosol samples was examined under different conditions, determining that hydrolysis of PAEs is most susceptible to the ambient temperature at typical atmospheric aqueous pH of 3–5. In addition, photodegradation results suggested that indirect photodegradation played a key role in photodecomposition of PAEs under sunlight irradiation. The photo-generated reactive species including triplet water-soluble organic matter ( 3 WSOM∗) and reactive oxygen species (ROS) mainly contribute to the degradation of PAEs in atmospheric aqueous aerosol. This study provides an important reference for better understanding the occurrence and transformations of PAEs in ambient urban atmospheric aqueous aerosol.
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Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Shunxi Zhang,Yongkang Mou,Yiyang Zhou,Hengyuan Tong,Hongyang Gong,Yun He,Wenjuan Shen,Jianfen Li
A micropore-adjusted gas diffusion electrode (MAGDE) is prepared using CBCNT (Mixture of carbon black and carbon nanotubes), polytetrafluorethylene (PTFE), wood pulp and filler. Meantime, an in-situ electrosynthesis process of hydrogen peroxide is constructed by a MAGDE cathode and a carbon felt anode. The results show that when the optimal mass ratio of PTFE:pulp:filler:CBCNT is 1.5:0.15:3:1, H 2 O 2 yield can reach to 99.8 mg/(L.cm 2 ) in 90 min. Under the optimal parameters of 60 mA/cm 2 and pH of 3, H 2 O 2 yield can reach to 114.4 mg/(L.cm 2 ). For 20 mA/cm 2 , current utilization efficiency and energy consumption are 76.5 % and 8.6 KWh/kg in 30 min, respectively. A series of tests show that when filler is added, surface porous characteristics, electroactive surface and oxygen transfer ability are all improved and possible mechanisms on H 2 O 2 yield increment are suggested. H 2 O 2 yield and wastewater treatment show that the prepared MAGDE is effective and the preparation process is feasible.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Yining Sun,Renguo Zhang,Chenglin Su,Qijun Sun,Shuang Cao,Meng Wu,Zichun Jin,Na Niu,Ligang Chen,Lan Ding
Biotic and abiotic stresses can disrupt plant metabolic processes. This leads to the excessive accumulation of hydrogen peroxide (H 2 O 2 ) in plants, which in turn induces oxidative stress. Therefore, detection of H 2 O 2 is critical to understanding plant growth. In this study, we developed a naphthalene-based fluorescein near-infrared fluorescent probe (NAPF-AC) for the sensitive and selective detection of H 2 O 2 . Upon exposure to H 2 O 2 , the probe undergoes disruption of its push-pull electronic structure, triggering an intramolecular charge transfer process that allows for fluorescence-based detection. NAPF-AC exhibited excellent linearity (R 2 = 0.998) over a wide concentration range of H 2 O 2 (0.1 to 100 μM), with a limit of detection (LOD) as low as 0.05 μM. In addition, NAPF-AC was successfully used for the in-situ detection of H 2 O 2 in plant tissues. This study provides a powerful tool for studying H 2 O 2 dynamics in plants and offers new insights into the mechanisms regulating plant growth and stress responses.
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LAND DEGRADATION & DEVELOPMENT
·
2025
·
IF 3.7
Sen Niu,Minghui Li,Yan Jiang,Yujun Wang,Qibiao Ren
Carbendazim, a commonly used fungicide, was identified as a contributor to soil degradation. Despite the extensive use of carbendazim, the degradation mechanisms and the removal process of carbendazim in the soil environment were not yet fully understood. A deeper understanding of the degradation pathways of carbendazim was considered essential for developing effective strategies to mitigate long-term environmental impacts. The degradation of carbendazim using ozone in water and soil environments was investigated with the simulation experiments of ozone exposure in water and soil, and the degradation pathways and removal efficiency of carbendazim in water were also explored under different conditions in the study. The results showed that ozone-induced degradation proceeded in three stages: rapid removal, slow removal, and equilibrium. In water, ozone directly reacted with carbendazim, generating hydroxyl radicals (˙OH), which oxidized carbendazim into intermediates, including 2-aminobenzimidazole, 2-hydroxybenzimidazole, 2-benzimidazolone, and o-phenylenediamine. The intermediates eventually degraded into nontoxic byproducts such as carbon dioxide and water. In soil, ozone treatment enhanced the removal of carbendazim by catalyzing the production of active substances, including hydroxyl radicals (˙OH) and hydrogen peroxide (H 2 O 2 ). Increasing the aeration frequency to four times daily significantly improved the efficiency of carbendazim removal, reaching the peak removal rate of 70.4% after 21 days of ozone exposure in the soil. The findings of this study clarified the theoretical mechanisms of ozone treatment for carbendazim remediation, highlighted the critical role of hydroxyl radicals (˙OH) in the degradation process, and established a scientific foundation for developing remediation strategies targeting carbendazim-induced soil contamination.
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PeerJ
·
2025
·
IF 2.4
Lijin Zhang,Aiping Lin,Jie Lin,Juan Chen,Mengshi Chen,Xunbin Yu,Yijuan Wu,Tao Wang,Yan Lu,Zhaofen Ba,Xiaowei Cheng,Tiantian Zhang,Minghong Chen
Background To assess the status of NEUROG1 methylation in the advanced adenoma and colorectal cancer. Methods The NEUROG1 methylation in tissue and stool samples from patients with colorectal cancer (CRC), advanced adenoma (AA), and non-advanced adenoma (NAA) were evaluated using methylation-specific quantitative polymerase chain reaction (PCR). Results In tissue samples, the NEUROG1 methylation detection rates were 36% for CRC, 24% for NAA, and 88% for AA. In stool samples, the NEUROG1 methylation detection had a sensitivity of 63.46% for CRC with a positive predictive value (PPV) of 85.94%. The overall diagnostic specificity of the test for the NAA and the healthy control was 76.32%, with a negative predictive value (NPV) of 40.28%. Conclusion NEUROG1 methylation detection can potentially be used in the CRC and AA screening.
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INDUSTRIAL CROPS AND PRODUCTS
·
2025
·
IF 6.2
Mei Chen,Fengyun Tian,Youshudi Xie,Jie Ma,Yanfen Yang,Changli Yang,Xinrong Zhou,Tian Deng,Houhong Xiao,Xue Dong,Dingli Chen,Xinlong Dai,Zhi zhou,Shimao Fang
Volatile esters play a crucial role in shaping the aroma profiles and sensory attributes of horticultural crops. In this study, we comprehensively investigated the volatile esters, alcohols, and acids profiles of different tea plant varieties and tissues to identify the key metabolites responsible for tea flavors. Subsequently, two homologous genes, CXE2 and CXE3 , which encode a carboxylesterase and are involved in the hydrolysis of esters, were screened from Camellia sinensis , Vaccinium darrowii , Myrica rubra , and Actinidia chinensis via multiomics analysis. In vitro , the purified recombinant enzyme CXE2 and CXE3 catalyze the hydrolysis of acetate esters into alcohols and acids. Molecular docking, site-directed mutagenesis, and enzyme activity analysis revealed that Tyr-15 and Gly-81 were the key amino acids that imparted hydrolytic activity to CsCXE2 , whereas Gly-81 and Gly-82 were the key amino acids that imparted hydrolytic activity to CsCXE3 . In vivo , the low expression of CsCXE2 and CsCXE3 led to decreased normal pollen and an increased presence of triangular pollen. In tender tea shoots, the results of gene silencing and withering treatments revealed that CsCXE2 and CsCXE3 disrupted acetate metabolism and altered the dynamic balance between green grassy and fruity aroma-related compounds by hydrolyzing acetate esters. Overall, this study highlights the significant potential of targeting CXE2 and CXE3 for precise flavor modulation in horticultural crop cultivation and processing, offering novel strategies to optimize the aroma profiles of horticultural crops in industrial applications.
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