MOLECULES
·
2025
·Vol. 30
·期 10
·2231
·
IF 4.6
Yanping Zhang,Liyuan Zhao,Xinsheng Wang,Chenxi Zhang,Haichao Zuo,Di Gao
This study investigated the correlation between thirteen inorganic elements, five key bioactive compounds, and environmental factors inPolygonum perfoliatumL. from fifteen different origins. Analyses were conducted using techniques such as ultrasound-assisted extraction, HPLC, ICP-AES, PCA, and HCA. The results indicate that the geographical origin significantly influences the contents of inorganic elements and bioactive compounds inPolygonum perfoliatumL., and a certain correlation exists among elements, compounds, and environmental factors. This research provides a theoretical foundation for the development and utilization ofPolygonum perfoliatumL.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Mengcong Zhang,Li Lu,Xiaowen Hu,Chengzhe Zhou,Cheng Zhang,Jiaxin Fang,Linyuan Duan,Bo Zhang,Yuqiong Guo
Goji ( Lycium barbarum L.) leaf tea (LBLT) has gained popularity among consumers owing to its pleasant aroma. Selecting optimal Goji cultivars for tea processing is critical for enhancing the quality of LBLT. This study investigated the aroma characteristics of Goji leaf white tea (LBLWT) and green tea (LBLGT) prepared from 25 Goji lines. LBLWT emitted woody, floral and fruity aromas, while LBLGT exhibited a distinct roasted corn aroma. The integrated application of variable importance in projection, odour activity value and partial least-squares regression analyses led to the identification of key aroma compounds, whose metabolic pathways were also illustrated. Using multi-criteria evaluation methods, excellent Goji lines suitable for processing LBLWT (Z20, Z48 and N1) and LBLGT (Z85, Z20, N1 and Z68) were screened. This study took the lead in adopting multi-criteria evaluation methods, screening high-quality Goji lines and providing theoretical basis for high-quality LBLT processing.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Tian-yu Liu,Zi-wei Han,Si-si Jia,Kai Ma,Mei Li,Xin Yi,Hua Zhu,Jia-hao Fan,Hong-wei Qiu,Guang-ping Lv,He Huang
Addressing microbial imbalance is a critical therapeutic strategy for ulcerative colitis (UC). Natural polysaccharides have attracted increasing attention for their potential in UC prevention. In this study, we purified and characterized an acidic heteropolysaccharide (LBP 2–1) from Lycium barbarum L. and systematically evaluated its therapeutic effects on UC. Monosaccharide composition analysis combined with methylation analysis and NMR spectroscopy revealed that LBP 2–1 possesses a complex structure. The backbone is →2)-α-L-Rha p -(1 → 4)-α-D-GalA p -(1 → 6)-β-D-Gal p -(1 → and branched at the O-3/O-4 positions of →3,6)-β-D-Gal p -(1 → and →2,4)-α-L-Rha p -(1 →. The branch chains consist of →5)-α-L-Ara f -(1 →, →3,5)-α-L-Ara f -(1 →, →3)-β-D-Gal p -(1 →, β-D-Gal p -(1 →, and α-L-Ara f -(1 →. Particularly, LBP 2–1 relieved the symptoms of weight loss and colon shortening, and reduced DAI and histopathological scores in UC mice. Furthermore, LBP 2–1 alleviated UC symptoms by enhancing microbiota diversity, increasing the abundance of beneficial bacteria (e.g., norank_f__Muribaculaceae ), and reducing harmful bacteria (e.g., Bacteroides ). These findings elucidate the structural characteristics of a novel acidic polysaccharide and confirm its therapeutic potential for UC treatment.
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Scientific Reports
·
2025
·Vol. 15
·期 1
·1-18
·
IF 3.9
Siddique Farzana,Ahmad Salman,Hussain Ashiq,Firdous Nida,Nisar Rizwan,Bilal Muhammad,Najam Ayesha,Salik Abdullah,Zia Muhammad,Elkhedir Abdeen Elsiddig
Yoghurt is a one of the commonly consumed nutritious dairy products, which lacks certain bioactive compounds that are present in abundance in many plants. While, a significant range of phytonutrients are present in the leaves of Moringa oleifera . Thus, the purpose of this work is to develop a functional yoghurt, which could promote the health of the consumers to its bioactive components. In the present work, the buffalo milk yoghurt supplemented with Moringa oleifera leaves powder [at 0% (T 0 ), 0.2% (T 1 ), 0.4% (T 2 ), 0.6% (T 3 ) and 0.8% (T 4 )] was prepared to check the variations in the physicochemical, bioactive, and antioxidant properties, during 21 days of refrigerated storage. The results showed that the fat content was decreased from T 0 (4.42%), to T 4 (3.37%), and decreased from 4.40 to 4.38% during storage period of 21 days. The highest protein content was observed in T 4 (4.15%), while the lowest in T 0 (3.61%), and this protein content also deceased during storage. The moisture content was high in T 0 and lowest in T 4 , while increased in all samples during the storage. The pH and total plate count decreased significantly from T 0 to T 4 , whereas with the storage the pH decreased and total plate count was increased. A significant increase in the TPC, TFC and antioxidant activity of yoghurt samples was observed, with increasing the level of incorporation of Moringa oleifera leaves powder, as T 4 presented the highest values for these parameters. However, as storage days passed from 0 to 21, the TPC, TFC and antioxidant activity of all the samples was significantly decreased. Minerals analysis also revealed a significant increase in the Ca, K, Na, Fe and Zn content of the yoghurt, as a result of increasing the level of Moringa oleifera leaves powder, as T 4 was found to be having the highest amounts of these minerals. Current study concludes that Moringa oleifera leaves powder could be an excellent fortification ingredient for the development of functional yoghurt, which due to the presence of bioactive compounds could provide pharmacological benefits.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2020
·
IF 5.162
Hong Yao,Lei Wang,Xin Tang,Zhuo Yang,Huanyong Li,Chongzhen Sun,Xiyang Wu,Dan Xu
In this research, two novel polysaccharides (S1 and S2) from Solanum nigrum L were extracted and purified. Then homogeneity, molecular weights, major chemical contents and monosaccharide compositions of S1 and S2 were determined. Then, the effects of S1 and S2 on human faecal microbial community and short-chain fatty acid production were investigated using an in vitro fermentation model. Results showed that S1 and S2 have different impacts on human gut microbiota in vitro . S1 selectively promoted the abundance of 9 genera and the production of propionic acid, butyric acid, isobutyric acid, valeric acid and isovaleric acid; while S2 selectively promoted the abundance of 8 genera and the production of acetic acid, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid and succinic acid. Also, S1 group had higher abundance of genera Butyricimonas and Megamonas and higher levels of lactic acid than S2; while S2 group had higher abundance of Megaphaera and higher levels of butyric acid, valeric acid, isobutyric acid, isovaleric acid and succinic acid comparably. We concluded that S1 and S2 may have potential prebiotic functions.
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FOOD RESEARCH INTERNATIONAL
·
2023
·
IF 8.1
Mengyuan Wang,Jingchao Kang,Lin Chen,Gongchen He,Yaping Liu,Xiaojing Fan,Xin Lv,Xinglian Xu,Guanghong Zhou,Xianchao Feng
As a natural antioxidant, Epigallocatechin-3-gallate (EGCG) needed to be added in high doses to maintain the quality of meat products. However, high doses of EGCG caused the excessive aggregation of myofibrillar protein (MP), which damaged the gel properties of MP gels. Therefore, the purpose of this study was to investigate the mitigation of EGCG-induced loss of MP gelling potential by L-Lysine (L-Lys). The results showed that the addition of 20 mM L-Lys induced excessive unfolding and loose aggregation of MP at 10 µmol/g EGCG, and hence, reducing the solubility (14.5%) and the tryptophan fluorescence, and forming a network structure with a large aperture. Therefore, the cooking loss was decreased from 29.20% to 15.13%, and the strength of MP gels was decreased from 0.35 N to 0.17 N. However, L-Lys hindered the hydrogen bonding interactions and hydrophobic interactions between MP and EGCG by competing the binding sites of MP at 50 µmol/g EGCG, which was supported by the Zeta potential, surface hydrophobicity, FTIR and molecular docking analysis. Thus L-Lys mitigated the protein aggregation caused by 50 µmol/g EGCG, improved the solubility (23.02%∼86.99%) and apparent viscosity, which were beneficial for the formation of a continuous network structure in MP gels. Therefore, the cooking loss of MP gels was decreased from 52.40% to 41.30%, and the gel strength was enhanced from 0.13 N to o.22 N with 20 mM L-Lys addition. The present study could provide a new strategy for increasing the amounts of EGCG in meat products without damaging the gel properties of meat products.
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JOURNAL OF BIOMATERIALS APPLICATIONS
·
2023
·
IF 2.9
Tian Yi Wu,Wen Jie Cao,Zi Ling Li,Yan Chun Gong,Xiang Yuan Xiong
Drugs with different solubility can be selectively embedded into polymersomes with the hydrophilic core and hydrophobic bilayer. Novel folate-targeted Pluronic/poly (D,L-lactide-b-glycolide) polymersomes were constructed and used for the co-delivery of paclitaxel (PTX) and doxorubicin (DOX) to improve their inhibitory effect over cancer cells. The particle size of blank polymersomes was mainly distributed below 125 nm. The release of PTX and DOX from polymersomes showed an initial burst release followed by a sustained and slow release. The in vitro cytotoxicity data showed that the targeted co-loaded polymersomes (PTX&DOX FA-Ps) exhibited better inhibitory effect than single-loaded polymersomes and free drugs did. Furthermore, PTX&DOX FA-Ps showed the synergistic therapeutic effect over OVCAR-3 cancer cells. The cellular uptake results also showed that folate modified polymersomes had excellent targeting performance. Therefore, the folate-targeted Pluronic/poly (D,L-lactide-b-glycolide) polymersomes have potential application value as novel drug carriers to co-deliver PTX and DOX.
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APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
·
2022
·Vol. 106
·期 7
·2619-2636
·
IF 5.56
Chen Yunni,Xu Chenglong,Yang Huilin,Liu Zhenying,Zhang Zhibin,Yan Riming,Zhu Du
Abstract Perylenequinones (PQ) are natural polyketides used as anti-microbial, -cancers, and -viral photodynamic therapy agents. Herein, the effects of L-arginine (Arg) on PQ biosynthesis of Shiraia sp. Slf14(w) and the underlying molecular mechanism were investigated. The total content of PQ reached 817.64 ± 72.53 mg/L under optimal conditions of Arg addition, indicating a 30.52-fold improvement over controls. Comparative transcriptome analysis demonstrated that Arg supplement promoted PQ precursors biosynthesis of Slf14(w) by upregulating the expression of critical genes associated with the glycolysis pathway, and acetyl-CoA and malonyl-CoA synthesis. By downregulating the expression of genes related to the glyoxylate cycle pathway and succinate dehydrogenase, more acetyl-CoA flow into the formation of PQ. Arg supplement upregulated the putative biosynthetic gene clusters for PQ and activated the transporter proteins (MFS and ABC) for exudation of PQ. Further studies showed that Arg increased the gene transcription levels of nitric oxide synthase (NOS) and nitrate reductase (NR), and activated NOS and NR, thus promoting the formation of nitric oxide (NO). A supplement of NO donor sodium nitroprusside (SNP) also confirmed that NO triggered promoted biosynthesis and efflux of PQ. PQ production stimulated by Arg or/and SNP can be significantly inhibited upon the addition of NO scavenger carboxy-PTIO, NOS inhibitor N ω -nitro-L-arginine, or soluble guanylate cyclase inhibitor NS-2028. These results showed that Arg-derived NO, as a signaling molecule, is involved in the biosynthesis and regulation of PQ in Slf14(W) through the NO-cGMP-PKG signaling pathway. Our results provide a valuable strategy for large-scale PQ production and contribute to further understanding of NO signaling in the fungal metabolite biosynthesis. Key points • PQ production of Shiraia sp. Slf14(w) was significantly improved by L-arginine addition. • Arginine-derived NO was firstly reported to be involved in the biosynthesis and regulation of PQ. • The NO-cGMP-PKG signaling pathway was proposed for the first time to participate in PQ biosynthesis.
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Food & Function
·
2020
·Vol. 11
·期 11
·10149-10160
·
IF 4.171
Yao Ren,Hui Wu,Yuanlong Chi,Ruijie Deng,Qiang He
Tilapia (Oreochromis nilotica L.) skin collagen is a meritorious commercial resource to be exploited. The purpose of this study was to obtain, evaluate, and characterize tilapia skin collagen-derived antioxidant hydrolysates (TSCP). AAPH-induced erythrocyte hemolysis assay and antifatigue test in mice were implemented. It was indicated that TSCP treatment at 1 mg mL−1 could effectively attenuate AAPH-induced erythrocyte hemolysis rate from 56.35 ± 2.46% to 18.78 ± 2.48% (p < 0.01). A 2.5 mg/(10 g d) dose of TSCP intragastric administration could observably prolong the exhaustive swimming time of the loaded mice and its mechanism was multiple, including the decrease in the levels of serum lactic acid, serum urea nitrogen, and creatine kinase activity, thus improving the contents of liver and muscle glycogen and endogenous SOD activity. Five oligopeptides from the antioxidant fraction were identified as Gly-Hyp, Glu-Asp, Asp-Hyp-Gly, Glu-Pro-Pro-Phe, and Lys-Pro-Phe-Gly-Ser-Gly-Ala-Thr and then synthesized. Among them, the octapeptide exhibited the strongest antioxidant capacity. Therefore, tilapia skin-derived collagen is a meritorious edible resource for producing commercial functional foods, thus helping to scavenge radicals, protecting erythrocytes, and further resisting fatigue.
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INDUSTRIAL CROPS AND PRODUCTS
·
2021
·
IF 5.645
Ru Zhao,Xinyu Yang,Muzhi Li,Xiaojin Peng,Mengxia Wei,Xiucheng Zhang,Lei Yang,Jialei Li
In order to produce an alternative energy of biodiesel using field pennycress ( Thlaspi arvense L.) seed oil, a novel Fe 3 O 4 /graphene oxide-phenylalanine bisulfate ionic liquid core-shell magnetic catalyst was developed. Ferroferric oxide nanoparticles and graphene oxide (GO) were employed to prepare a magnetic carrier of Fe 3 O 4 @GO nanocomposite which applied to immobilize phenylalanine bisulfate ionic liquid (PBIL). The immobilized PBIL was characterized by the means of characterization. The heterogeneous Fe 3 O 4 @ [email protected] catalyst was demonstrated to have a high catalytic efficiency that reached 92.38 % of biodiesel yield under the optimal conditions (methanol/oil molar ratio of 10:1, reaction temperature of 60 °C, reaction time of 4 h, catalyst dosage of 25 wt%, stirring speed of 1500 r/min). After transesterification processes, the catalyst could be easily removed from the reaction mixture with an external magnet and showed little deactivation after multiple recycles. The main properties of biodiesel product were reconcilable with the range of test standards of ASTM D6751 and EN 14214. In summary, the prepared Fe 3 O 4 @ [email protected] catalyst behaved a superiorly catalytic performance, which made the produced biodiesel possess the possibility of application to biofuel as an ideal substitution.
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JOURNAL OF FOOD COMPOSITION AND ANALYSIS
·
2024
·
IF 4
Yanbo Hu,Jianing Zhou,Xianjun Zou,Ruining Zhang,Yi Cao,Yuzhu Shen,Jun Zhao
Dietary polysaccharides, widely present in plants, fungi, and algae, have been utilized as functional foods because of their excellent bioactivity. In this study, four polysaccharides (BSPS) from black soybean hulls, designated as BSPS-N-a, BSPS-N-b, BSPS-A-c, and BSPS-A-d, were efficiently extracted and purified. Their fine structures were subsequently investigated through a combination of Carbon-13 Nuclear Magnetic Resonancea ( 13 C NMR), Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF-MS), and enzymatic hydrolysis. Monosaccharide analysis, Fourier transform infrared spectroscopy (FT-IR) analysis, and 13 C NMR analysis indicated that BSPS-N-a was a galactomannan composed of β-1,4-D-Man p and β-1,4-D-Gal p , BSPS-N-b was a galactoglucomannan composed of β-1,4-D-Gal p , β-1,4-D-Glc p and β-1,4-D-Man p ; Both BSPS-A-c and BSPS-A-d were composed of homogalacturonan (HG) with minor rhamnogalacturonan-I (RG-I) domains composed of α-1,4-D-Gal p A and β-1,4-D-Gal p , the difference was that BSPS-A-c exhibited a higher degree of branching, mainly containing a large amount of α-1,2,4-L-Rha p . To verify the results of 13 C NMR, enzymatic hydrolysis combined with MALDI-TOF-MS analysis was determined. The results showed that BSPS-N-a and BSPS-N-b could be degraded into hexose (Hex) with a degree of polymerization ranging from 2 to 9 by mannanase, and BSPS-A-c and BSPS-A-d were degraded into galacturonic acid (GalA) with a degree of polymerization ranging from 2 to 3 by endo-polygalacturonase. These results were consistent with the above results from monosaccharide analysis, FT-IR analysis, and 13 C NMR analysis. This study will provide a basis for the structure-activity relationships of black soybean hull polysaccharides (BSPS) and the development of BSPS as new functional dietary polysaccharides, natural functional foods and pharmaceuticals.
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INDUSTRIAL CROPS AND PRODUCTS
·
2019
·
IF 4.191
Xianchao Shang,Yuqing Dou,Yingjie Zhang,Jia-Neng Tan,Xinmin Liu,Zhongfeng Zhang
Natural deep eutectic solvents (DESs) have received increasing attention in recent years as emerging green solvents for efficient extraction of bioactive compounds from natural resources. In this study, we aimed to evaluate the potential and effectiveness of DESs with different polarities for extraction of isoflavones from chickpea sprouts. To this end, we examined 20 different DESs based on choline chloride, betaine, and l -proline as hydrogen bond acceptors in combination with different hydrogen bond donors (carboxylic acids, alcohols, sugars, and amine). Extraction efficiency was evaluated based on the extraction yields of four isoflavones (ononin, sissotrin, formononetin, and biochanin A), total flavonoid content, and anti-oxidant activity. On the basis of the results obtained, choline chloride-based DESs containing propylene glycol as a hydrogen bond donor (molar ratio of 1:1) with 30% (v/v) of water was the most effective at extracting isoflavone compounds from chickpea sprouts, compared to conventional solvents. Subsequently, in order to examine the factors contributing to optimal ultrasound-assisted extraction conditions, a Box-Behnken design paired with response surface methodology was implemented. DES water content and extraction temperature were accordingly found to have prominent effects on the extraction yields of isoflavones. Our results indicate that DESs can be used as suitable green solvents for extracting bioactive compounds from various biomaterials.
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