MICROCHIMICA ACTA
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2023
·Vol. 191
·期 1
·1-11
·
IF 5.7
Guo Zhanghong,Zhou Lin,Chen Xuan,Song Qijun
Carbon-coated copper nanocrystals (CuNCs) with peroxidase-like activity were hydrothermally prepared by using copper acetate, citric acid (CA) and histidine (His) as the precursors. Various shaped CuNCs, including urchin-like, slab-like and spherical appearance were facilely prepared by addition of different amount of NaNO 2 in the precursor solutions. When 3,3′,5,5′-tetramethylbenzidine (TMB) was used as the substrate, the CuNCs with urchin-like appearance have greatest peroxidase-like activity and their Michaelis–Menten constant ( K m ) and the maximum rate constant (ν max ) are respectively 8.8 and 1.2 times higher than that obtained from horseradish peroxidase (HRP). The production of reactive oxygen species (ROS) was confirmed by radical quenching and electron spin resonance (ESR) tests. Subsequent studies have found that the CuNCs catalyzed color reaction of TMB can be selectively quenched by the environmental pollutant 2,4-dinitrophenylhydrazine (2,4-DNPH). Thus a new colorimetric method for the determination of 2,4-DNPH with a linear range of 0.60–20 µM was developed and a limit of detection (LOD) as low as 0.166 µM was achieved. The results obtained not only reveal the tunability of the peroxidase-like activity of Cu-based nanomaterials, but also provide a new method for the sensitive determination of environmental contaminate. Graphical abstract
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MOLECULAR IMAGING AND BIOLOGY
·
2023
·
IF 3.1
Li Yiran,Jin Xiaofei,Wang Feilong,Zhou Huijing,Gu Yueqing,Yang Yamin,Qian Zhiyu,Li Weitao
Purpose The data acquisition of drug metabolism analysis requires a lot of time and animal resources. However, there are often many deviations in the results of pharmacokinetic analysis. Conventional methods cannot measure the blood drug concentration data in multiple tissues at the same time, and the data is obtained by in vitro measurement, which produces time errors, in vitro data errors, and individual differences between animals. In the analysis of pharmacokinetic parameters, it will seriously affect the pass rate of clinical trials of R&D drugs and the accuracy of the dosing schedule. To the best of our knowledge, we have not found the study of in vivo blood drug concentration using multi-channel equipment. Therefore, the purpose of this paper is to build a set of multi-organ monitoring and analysis instruments for synchronously monitoring the metabolism of drugs in various tissues of small animals, so as to obtain real in vivo data of blood drug concentration in real time. Procedures Using the fluorescence properties and laser-induced fluorescence principle of drugs, we designed six channels to monitor the changes of fluorescence-labeled drugs in their main metabolic organs, a multi-channel calibration method was proposed to improve the accuracy of the time-division multiplexing, the real-time collection of drug concentration in vivo is realized, and the drug metabolism curve in vivo can be observed. Results The instrument satisfies the collection of small doses of drugs such as microgram; the detection sensitivity can reach 10 ng/ml, and can monitor and collect the drug metabolism of multiple small animal tissues at the same time, which greatly reduces the use of animals, reduces the differences between individuals, and reduces consumption cost and improve the detection efficiency of parameters, and obtain data information that is closer to the real biology. Conclusion The real-time continuous monitoring and data collection of the drug metabolism in the plasma of living small animals and the important organs such as kidney, liver, and spleen were realized. The research and development of new drugs and clinical research have higher practical value.
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ANALYTICAL SCIENCES
·
2023
·
IF 1.6
Xu Yang,Liu Yue,Luo Yu,Xu Xinlin,Li Yingying,Zhao Lin,Li Tiantian,Zhang Yan,He Peng,Mou Xiaoming
Lysozyme (LYS) is a widely used bacteriostatic enzyme. In this paper, we built a sensitive and accurate Raman biosensing platform to detect trace amounts of LYS. The method is based on magnetic spherical nucleic acid formed by a combination of LYS aptamer (Apt) and magnetic beads (MBs). Meanwhile, this method utilizes a dual enzyme-assisted nucleic acid amplification circuit and surface-enhanced Raman scattering (SERS). In this sensing strategy, which is based on the specific recognition of Apt, magnetic spherical nucleic acids were associated with SERS through a nucleic acid amplification circuit, and the low abundance of LYS was converted into a high-specificity Raman signal. Satellite-like MB@AuNPs were formed in the presence of the target, which separated specifically in a magnetic field, effectively avoided the interference of complex sample environment. Under the optimal sensing conditions, the concentration of LYS exhibited a good linear relationship between 1.0 × 10 –14 and 5.0 × 10 –12 M and the limit of detection was as low as 8.3 × 10 –15 M. In addition, the sensor strategy shows excellent accuracy and sensitivity in complex samples, providing a new strategy for the specific detection of LYS. Graphical abstract
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JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-17
·
IF 10.2
Pan Qi,Li Ke,Kang Xueqin,Li Kaixuan,Cheng Zihe,Wang Yafei,Xu Yuye,Li Lei,Li Na,Wu Guilong,Yang Sha,Qi Shuo,Chen Guodong,Tan Xiaofeng,Zhan Yonghua,Tang Li,Zhan Wenhua,Yang Qinglai
Orthotopic advanced hepatic tumor resection without precise location and preoperative downstaging may cause clinical postoperative recurrence and metastasis. Early accurate monitoring and tumor size reduction based on the multifunctional diagnostic-therapeutic integration platform could improve real-time imaging-guided resection efficacy. Here, a Near-Infrared II/Photoacoustic Imaging/Magnetic Resonance Imaging (NIR-II/PAI/MRI) organic nanoplatform IRFEP-FA-DOTA-Gd (IFDG) is developed for integrated diagnosis and treatment of orthotopic hepatic tumor. The IFDG is designed rationally based on the core “S-D-A-D-S” NIR-II probe IRFEP modified with folic acid (FA) for active tumor targeting and Gd-DOTA agent for MR imaging. The IFDG exhibits several advantages, including efficient tumor tissue accumulation, good tumor margin imaging effect, and excellent photothermal conversion effect. Therefore, the IFDG could realize accurate long-term monitoring and photothermal therapy non-invasively of the hepatic tumor to reduce its size. Next, the complete resection of the hepatic tumor in situ lesions could be realized by the intraoperative real-time NIR-II imaging guidance. Notably, the preoperative downstaging strategy is confirmed to lower the postoperative recurrence rate of the liver cancer patients under middle and advanced stage effectively with fewer side effects. Overall, the designed nanoplatform demonstrates great potential as a diagnostic-therapeutic integration platform for precise imaging-guided surgical navigation of orthotopic hepatic tumors with a low recurrence rate after surgery, providing a paradigm for diagnosing and treating the advanced tumors in the future clinical translation application. Graphical Abstract
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CATALYSIS LETTERS
·
2023
·
IF 2.8
Ji Yaxiong,Lin Shuang,Xu Guihong,Chen Tianen,Gong Jianchao,Meng Fanbin,Wang Yuanhao
Abstract Catalytic conversion of CO 2 to methanol with H 2 from renewable energy has attracted increasing interest as a promising strategy for reducing excessive CO 2 emissions. However, the performance of reported various catalysts still suffers from low methanol yield with a passable CO 2 conversion. In this work, Cu–ZnO–La 2 O 3 interfaces are constructed with various La 2 O 3 mole ratio. Compared to Cu/ZnO, the optimized catalyst (i.e., Cu 0.6 Zn 0.2 La 0.4 ) exhibits a much higher mass-specific methanol formation rate (159.3 g MeOH /kg cat /h) at 240 °C and 3 MPa. A series of ex-situ and in-situ characterizations, such as X-ray diffractometer (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM), N 2 O titration measurements, and in-situ diffuse reflectance Fourier transform infrared spectroscopy ( in-situ DRIFTS) study, are used to investigate its structure and mechanism study. The dispersion of Cu over Cu/ZnO/La 2 O 3 catalyst is significantly enhanced, forming more Cu–ZnO–La 2 O 3 interfaces. LaO x species favor CO 2 activation and generate more carbon intermediates species for CO 2 hydrogenation. Furthermore, more Cu + is bonded, which stabilizes the key intermediate, inhibits its desorption, and facilitates its further hydrogenation to methanol. This work is expected to offer an effective strategy to develop new catalysts with high performance for CO 2 hydrogenation to methanol. Graphical Abstract
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RESEARCH ON CHEMICAL INTERMEDIATES
·
2022
·Vol. 48
·期 3
·1181-1198
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IF 3.134
Zhang Zhongze,Liu Zonghui,Gu Zhiyuan,Wen Zhe,Xue Bing
The catalytic transfer hydrogenation (CTH) reaction of ethyl levulinate (EL) with alcohol to γ-valerolactone (GVL) has been investigated over a series of Zr-based catalysts, including ZrO 2 , ZrP, Zrβ, ZrP/Hβ and ZrO 2 /HZSM-5. It is found that the simplest ZrO 2 was the most efficient for GVL production, giving an EL conversion as high as 97% and GVL selectivity of 91% after 7 h at 170 °C using 2-propanol as the hydrogen donor and solvent. The effects of reaction temperature, time and solvent on catalytic performance were investigated. Moreover, the kinetic behavior of CTH reaction of EL was also studied over ZrO 2 catalyst. The as-prepared ZrO 2 gives an Ea value of 32.9 kJ/mol, which is much lower than other catalysts in the literatures. Furthermore, those catalysts have been, respectively, characterized by XRD, IR, BET and NH 3 -TPD techniques to reveal the physical properties and structures of these materials. Correlating the catalyst performance with its physical and chemical properties uncovers that the higher pore diameter, lower total acidity and higher Lewis acid sites of catalyst surface would be the key to the catalyst performance. Graphical abstract
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CATALYSIS LETTERS
·
2022
·Vol. 153
·期 4
·1170-1179
·
IF 2.936
Luo Mingsheng,Li Hong,Song Huanqiao,Shao Changke,Wang Aimei
The alkylation of phenol with methanol was studied in vapor phase over 5 wt% cerium supported on HZSM-5 catalyst prepared by three different methods, such as impregnation, ball mixing and solid-state ion-exchange. The catalytic activities are tested in a fixed-bed reactor, and the physicochemical properties of the catalyst are characterized with X-ray powder diffraction, scanning electron microscope, nitrogen physical sorption, Fourier Transform Infrared Spectrometer, Infrared spectra of pyridine adsorption, X-ray photoelectron spectroscopy and temperature-programmed desorption of ammonia. It was found that the dispersion form of cerium was greatly affected by the preparation method, and difference in acidity affects catalytic performance in the alkylation reaction of phenol and methanol. Ball milling method generated greater amount of strong Brønsted acidic sites and lower specific surface area than other catalysts, which led to higher carbon deposition rate and lower catalyst activity. The catalyst prepared by impregnation method yields higher Lewis acid concentration (2.09 μmol/g) and more chemisorbed oxygen due to the increased Ce 3+ concentration in the lattice, resulted in an improved o-cresol selectivity of 48%. Furthermore, the catalyst prepared by solid-phase ion-exchange method produces a catalyst with appropriate B/L ratio of 60.6 and large specific surface area of 389.0 m 2 /g, which yields the highest phenol conversion of 23%. Graphical Abstract
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JOURNAL OF MATERIALS SCIENCE
·
2022
·Vol. 57
·期 27
·12886-12893
·
IF 4.682
Ullah Wajid,Aziz Tariq,Ali Amjad,Khan Zaheen Ullah,Das Sandeep Kumar,Iqbal Mudassir,Ullah Roh,Wazir Nasrullah,Khan Farman Ullah,Raheel Muhammad
Solar photo-catalysis is very essential for both harvesting high-density solar energy, and environmental sustainability. A high surface area carbon nitride (HSACN), a photo-catalyst is synthesized by simple re-crystallization and calcination methods. The decrease in the size of urea crystals is ascribed to the use of different solvents and direct calcination that resulted in the formation of carbon nitride. The re-crystallization suggests that the defect-rich carbon nitride created nitrogen vacancies, which accelerated the photocatalytic activity of dyes’ degradation. The synthesized materials were characterized by X-ray powder diffraction, Field emission scanning electron microscopy, and transmission electron microscopy for structural and morphology evaluation. The corresponding high surface area carbon nitride demonstrated higher photocatalytic-dye-degradation efficiency under UV light irradiation, and higher adsorption capacity compared to graphitic carbon nitride, which is obtained without re-crystallization protocol. An increase in the surface area of HSACN has been observed due to structural disturbance caused by the growth of tiny urea crystals in carbon nitride. The nitrogen vacancies incorporation improved the absorption of extended visible light. The enhancement in photocatalytic activities are corresponding to the improved charge carrier photoexcitation, separation, and generated abundant reaction sites.
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BMC NEUROSCIENCE
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2022
·Vol. 23
·期 1
·1-16
·
IF 3.264
Wang Jie,Xia Zihao,Sheng Peng,Shen Mengmeng,Ding Lidong,Liu Dezhi,Yan Bing Chun
Background Neuronal injury induced in young rats by cerebral ischemia reperfusion (CIR) is known to differ substantially from that in adult rats. In the present study, we investigated the specific differences in neuronal injury induced by focal CIR between young and adult rats. Results 2, 3, 5-triphenyl tetrazolium chloride (TTC) staining revealed a gradual increase in the infarct volume of both young and adult rats in accordance with I/R times and was significantly lower in young rats than in adult rats under the same conditions. The number of cells in the cortex showing immunoreactivity for neuronal nuclei (NeuN) gradually decreased in both young and adult rats in accordance with I/R times; these numbers were significantly higher in young rats than in adult rats under the same conditions. Similarly, as the duration of I/R increased, the degree of glial activation in the cortex penumbra region became more severe in both young and adult groups; however, glial activation was significantly lower in the cortex penumbra region of young rats when compared with that in adult rats. In addition, the expression of Beclin-1 was significantly higher in the infarct penumbra of young rats than adult rats and was more frequently co-expressed with neurons. The levels of autophagy-related proteins increased significantly in the penumbra region after I/R in both young and adult groups, this increase was more pronounced in young rats than in adult rats. Following CIR, analysis revealed significantly lower levels of pro-apoptosis-related factors and significantly higher levels of anti-apoptosis-related proteins in the young rats than in adult rats. Conclusions Collectively, the present results suggest that the the reduced levels of neuronal death after CIR in young rats were closely related to enhanced levels of autophagy and reduced levels of pro-apoptosis in neurons.
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MICROCHIMICA ACTA
·
2021
·Vol. 188
·期 10
·1-10
·
IF 5.833
Cheng Sijie,Zhang Junqiu,Liu Yaoming,Wang Yingte,Xiao Yanteng,Zhang Yong
Water soluble N, S-doped carbon dots (N, S-CDs) with orange emission were synthesized from basic fuchsin and sulfosalicylic acid by the typical hydrothermal route. Based on the inner filter effect (IFE), the prepared N, S-CDs can be innovatively developed as an effective “signal-off” multifunctional sensing platform for sensitive determination of tetracycline antibiotics (for example, chlortetracycline (CTC)) and quercetin. The proposed sensor was utilized to realize the determination of CTC in water and milk samples and quercetin in beer sample (λex = 375 nm, λem = 605 nm) with satisfactory recoveries and relative standard deviations (RSD). The linear range and detection limit (LOD) of CTC is 1.24–165 μM and 32.36 nM, respectively. For quercetin, the linear ranges are 0.98–34 μM and 34–165 μΜ, and the LOD is 6.87 nM (3σ/m). By virtue of the good biocompatibility and long-wavelength emission, N, S-CDs were also used in the imaging of oocystis cells and yeast cells, which demonstrated promising applicability for bio-imaging and sensing. Graphical abstract
In this paper, N, S-doped carbon dots (N, S-CDs) with orange emission (λem = 605 nm) were synthesized from basic fuchsin and sulfosalicylic acid. Based on the inner filter effect (IFE), the prepared N, S-CDs can be innovatively developed as an effective “signal-off” multifunctional sensing platform for the sensing of tetracycline antibiotics (for example: chlortetracycline (CTC)) and quercetin. The sensor has been successfully applied to the determination of CTC in water and milk samples and quercetin in beer sample (λex = 375 nm, λem = 605 nm). The linear range and detection limit (LOD) of CTC is 1.24-165 μM and 32.36 nM respectively. For quercetin, the linear ranges are 0.98-34 μM and 34-165 μΜ, and the LOD is 6.87 nM (3σ/m). In addition, due to the characteristics of good biocompatibility and long-wavelength emission, the N, S-CDs were also used in the imaging of oocystis cells and yeast cells, which demonstrated promising applicability for bioimaging and sensing.
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BMC Complementary Medicine and Therapies
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2026
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IF 4.4
Zou Xian-Liang,Deng Gang,Wu Jian-Hua,Zhang Ting,Wang Yong-Li,Chou Gui-Xin
Background Rehmanniae Radix (RR), obtained from Rehmannia glutinosa , is widely used in traditional Chinese medicine. Phenylethanoid glycosides are relatively stable during processing and represent important bioactive constituents of Rehmanniae Radix Praeparata (RRP). This study aimed to establish an integrated analytical strategy combining ultra-performance liquid chromatography–electrospray ionization mass spectrometry (UPLC-ESI-MS), quantitative analysis of multi-components with a single marker (QAMS), and high-performance liquid chromatography (HPLC) fingerprinting for comprehensive quality evaluation of RRP. Methods UPLC-ESI-MS was used to identify the major constituents of RRP. Seven phenylethanoid glycosides were quantitatively determined by a validated UPLC-photodiode array (UPLC-PDA) method using QAMS with acteoside as the internal reference. Relative correction factors (RCFs) obtained by the average-value and linear regression methods were compared with those of the external standard method (ESM). HPLC fingerprint analysis was performed to evaluate batch-to-batch consistency. Results Eight compounds were identified in RRP, and seven phenylethanoid glycosides were quantitatively determined using the QAMS method with acteoside as the internal reference. The method showed excellent linearity (r² ≥ 0.9994), good precision, stability, and recovery (98.62%–102.64%), and the QAMS results were in good agreement with those obtained by ESM. HPLC fingerprint analysis of 17 batches of RRP showed similarity values ranging from 0.914 to 0.986, indicating good batch-to-batch consistency. Conclusion The proposed UPLC-PDA- and QAMS-based method, combined with HPLC fingerprint analysis, provides a practical, reliable, and cost-effective strategy for quality evaluation of RR and RRP.
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Nature Communications
·
2026
·
IF 18.1
Hu Yingjie,Long Yongde,Mao Baoguang,Yu Zeqiang,Zhao Linjie,Tang Cheng,Kou Ruitian,Sun Chaoyang,Hu Chuangang
The oxide path mechanism offers vision possibility from kinetics for the anodic oxygen evolution reaction, yet its universal activation remains elusive due to the difficulty of constructing globally appropriate interatomic distances. Here, we address this challenge in the integrated-screened Y 0.1 Ir 0.2 Ru 0.7 O x by precisely regulating the Ru-Ru 1 bond length and constructing locally ordered yet long-range disordered structures that dissipate lattice strain, thereby enabling homogeneous modulation of interatomic distances across the framework. In-situ infrared spectroscopy together with online mass spectrometry confirms that the catalyst consistently follows a universal pathway. Building on these structural and mechanistic advantages, the nanostructured catalyst achieves a low cell voltage of 1.75 V@3 A cm -2 with operational stability ( > 2800 h @ 3 A cm -2 , 0.0625 mV h -1 decay rate) in proton exchange membrane water electrolysis. This work establishes a generalizable framework for pervasive dual-site catalysis, providing a paradigm for next-generation water electrolysis anodes and beyond.
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