International Journal of Nanomedicine
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2017
·
IF 4.3
Guangtao Xu,Huan Gu,Bo Hu,Fei Tong,Daojun Liu,Xiaojun Yu,Yongxia Zheng,Jiang Gu
Brain ischemia/reperfusion (I/R) injury (BI/RI) is a leading cause of death and disability worldwide. However, the outcome of pharmacotherapy for BI/RI remains unsatisfactory. Innovative approaches for enhancing drug sensitivity and recovering neuronal activity in BI/RI treatment are urgently needed. The purpose of this study was to evaluate the protective effects of tumor necrosis factor (TNF)-α-loaded poly(ethylene glycol)-b-(poly(ethylenediamine L-glutamate)-g-poly(L-lysine)) (TNF-α/PEG-b-(PELG-g-PLL)) nanoparticles on BI/RI. The particle size of PEG-b-(PELG-g-PLL) and the loading and release rates of TNF-α were determined. The nanoparticle cytotoxicity was evaluated in vitro using rat cortical neurons. Sprague Dawley rats were preconditioned with free TNF-α or TNF-α/PEG-b-(PELG-g-PLL) polyplexes and then subjected to 2 hours ischemia and 22 hours reperfusion. Brain edema was assessed using the brain edema ratio, and the antioxidative activity was assessed by measuring the superoxide dismutase (SOD) activity and the malondialdehyde (MDA) content in the brain tissue. We further estimated the inflammatory activity and apoptosis level by determining the levels of interleukin-4 (IL-4), IL-6, IL-8, IL-10, and nitric oxide (NO), as well as the expression of glial fibrillary acidic protein (GFAP), intercellular adhesion molecule-1 (ICAM-1), and cysteine aspartase-3 (caspase-3), in the brain tissue. We provide evidence that TNF-α preconditioning attenuated the oxidative stress injury, the inflammatory activity, and the apoptosis level in I/R-induced cerebral injury, while the application of block copolymer PEG-b-(PELG-g-PLL) as a potential TNF-α nanocarrier with sustained release significantly enhanced the bioavailability of TNF-α. We propose that the block copolymer PEG-b-(PELG-g-PLL) may function as a potent nanocarrier for augmenting BI/RI pharmacotherapy, with unprecedented clinical benefits. Further studies are needed to better clarify the underlying mechanisms.
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BIOTECHNOLOGY AND BIOENGINEERING
·
2026
·
IF 3.9
Shu-Fang Li,Ting Zou,Sen-Yu Fu,Jin-Chuan Li,Yuan Li,Ya-Ru Shen,Ya-Ping Xue,Yu-Guo Zheng
The stability-activity trade-off remains a major bottleneck in engineering robust biocatalysts for industrial applications. Here, we developed an integrated computational strategy to simultaneously enhance the catalytic activity and thermostability of 7α-hydroxysteroid dehydrogenase (7α-HSDH) by engineering highly flexible regions, with a particular focus on α-helices. Evolutionary conservation analysis combined with multi-temperature molecular dynamics (MD) simulations was first applied to identify weakly conserved and flexible α-helices. Subsequently, computational tools (PROSS and Pythia) were utilized to generate mutations within these flexible regions. Through modular assembly of synergistic mutations, the optimal mutant M9 (D12K/V15I/V50E/I56R/D66A/T76R/A126I/A132H/S158A/S161A/A203P/H208Q) was obtained. Compared with the WT, M9 exhibited a 10.5°C increase in melting temperature ( T m ), a 2.3-fold prolongation of half-life ( t 1/2 ) at 40°C (reaching 75.3 h), a 3.14-fold improvement in catalytic efficiency ( k cat /K m ) to 29,032.73 mM −1 s −1 , and enhanced substrate tolerance. Structural and molecular dynamics analyses indicated that the enhanced performance of M9 was associated with increased rigidity of key α-helix regions, redistribution of surface electrostatic potential, and remodeling of the substrate-binding pocket. This study thus offers a novel design framework for improving the catalytic activity, thermostability, and substrate tolerance, balancing the stability-activity trade-off.
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CHEMICAL ENGINEERING JOURNAL
·
2018
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IF 6.735
Meng Li,Shaoqi Zhou
Microbial fuel cell (MFC) as a promising technology, which can be used to remove pollutant and generate bioelectricity simultaneously. In this study, the carbon cloth modified with α-Fe 2 O 3 /polyaniline nanocomposites is used as cathode in a dual chamber MFC for the generation of bioelectricity and electrochemical reduction of hexavalent chromium (Cr(VI)). The carbon cloth decorated with nanocomposites is prepared by a facile electrochemical polymerization technique. The MFC exhibits a complete Cr(VI) reduction at 50 mg·L −1 within 36 h, acquiring the obviously high Cr(VI) reduction rate of 1.39 g·m −3 ·h −1 , and the reduction rate is 1.67 times as high as that of the MFC-Blank (0.83 g·m −3 ·h −1 ). The MFC with α-Fe 2 O 3 /polyaniline nanocomposites cathode improves the Cr(VI) reduction rate and output power in MFC. And the maximum power density of this MFC is 1502.78 mW·m −2 , which is 1.753 times as high as that of the blank cathode. The improvement is due to the strong conductivity, high mobility of charge carriers and excellent electrochemical performance of nanocomposites. Besides, the power density of MFC modified with α-Fe 2 O 3 /polyaniline cathode has merely a slight decrease (2.23%) during fifty cycle operation due to the formation of protection layer. Consequently, the carbon cloth attached with α-Fe 2 O 3 /polyaniline nanocomposites with excellent electrochemical performance can be used as a promising cathode for Cr(VI) reduction and bioelectricity generation simultaneously.
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INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
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2021
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IF 5.277
Di Sun,Jin Mao,Zhijian Wang,Hui Li,Liangxiao Zhang,Wen Zhang,Qi Zhang,Peiwu Li
Peanut is an important resource of edible oil and digestible protein in daily life, which is rich in the nutriments and antioxidants such as vitamins, minerals and polyphenols. However, peanut is susceptible to the contamination of Aspergillus flavus ( A. flavus ), which can produce highly carcinogenic toxins that brings serious threats to human health and food safety. Exploring green and effective methods to control A. flavus is meaningful. Herein, a green and economical way to control A. flavus on peanuts was demonstrated. It was found that the growth of A. flavus hyphae and germination of its spores could be inhibited in the presence of α-Fe 2 O 3 nanorods under sunlight irradiation according to the agar diffusion method , flat colony counting method and fluorescence-based live/dead test. The diameter of inhibition zone was 22.3 ± 0.2 mm and the inhibition rate of spores germination was about 60 ± 5%, when the concentration of α-Fe 2 O 3 was 10 mg/mL for 7 h sunlight irradiation. Most important, α-Fe 2 O 3 showed the photocatalytic inhibition of A. flavus on peanuts under sunlight irradiation with the inhibition rate of about 90 ± 5%, and the production of aflatoxin B 1 and aflatoxin B 2 were reduced by 90 ± 2% and 70 ± 3%, respectively. By comparing the fat contents, protein contents, acid value, peroxide value and antioxidative compositions of peanuts, it was found that there was no obvious effect on the quality of peanuts after inhibition treatment. The findings provide a green, safe and economical strategy to control A. flavus on peanuts, which may be as a promising way to be used in food and agro-food preservation.
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Journal of Advanced Ceramics
·
2022
·Vol. 11
·期 2
·308-320
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IF 11.534
Wu Jian,Li Liangyu,Li Xing-ao,Min Xin,Xing Yan
Silver vanadates are promising visible-light-responded photocatalysts with suitable bandgap for solar absorption. However, the easy recombination of photogenerated carriers limits their performance. To overcome this obstacle, a novel 2D graphene oxide (GO) modified α-AgVO 3 nanorods (GO/α-AgVO 3 ) photocatalyst was designed herein to improve the separation of photocarriers. The GO/α-AgVO 3 was fabricated through a facile in-situ coprecipitation method at room temperature. It was found that the as-prepared 0.5 wt% GO/α-AgVO 3 exhibited the most excellent performance for rhodamine B (RhB) decomposition, with an apparent reaction rate constant 18 times higher than that of pure α-AgVO 3 under visible-light irradiation. In light of the first-principles calculations and the hetero junction analysis, the mechanism underpinned the enhanced photocatalytic performance was proposed. The enhanced photocatalytic performance was ascribed to the appropriate bandgap of α-AgVO 3 nanorods for visible-light response and efficient separation of photocarriers through GO nanosheets. This work demonstrates the feasibility of overcoming the easy recombination of photogenerated carriers and provides a valuable GO/α-AgVO 3 photocatalyst for pollutant degradation.
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DALTON TRANSACTIONS
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2017
·Vol. 46
·期 32
·10558-10563
·
IF 4.029
Dejian Yan,Jikai Liu,Zhichao Shang,He'an Luo
Ti-doped α-Fe2O3 nanorods (NRs) with carbon layer coating were fabricated for photoelectrochemical (PEC) water oxidation. The α-Fe2O3 NRs were grown on the surface of a Ti foil substrate by hydrothermal synthesis. Ti4+ was diffused from the Ti substrate and doped into the α-Fe2O3 NRs by sintering at 800 °C. The presence of Fe2+ in the α-Fe2O3 lattice was achieved by annealing the NRs in a lack of oxygen atmosphere, e.g. in argon. The co-existence of Ti4+ and Fe2+ results in significant enhancement of the PEC performance compared with the hematite NRs obtained by annealing in air, showing the absence of Fe2+. The carbon layer coating was conducted by the carbonization of glucose. Impressively, the coated carbon layer can not only facilitate the charge transfer of the photogenerated carriers but also effectively restrain the structural aggregation of the NRs upon high temperature sintering. The carbon layer coated NRs exhibited 1.2 times higher photocurrent density than the uncoated NRs due to the reduced charge recombination and well-distinct NR structures.
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Journal of Analysis and Testing
·
2024
·
IF 5.5
Zhang Hui,Chen Guilin,Zhang Yongli,Muema Felix Wambua,Ding Jun,Guo Mingquan
With the growing prevalence of diabetes and obesity worldwide, the role of α -glucosidase and lipase inhibitors has gained increasing concentration. Mulberry, a functional medicinal and edible plant, has been reported extensively in alleviating both diabetes and obesity. However, there is still a lack of refined research on its active ingredients and mechanisms. In our study, the inhibitory activities of mulberry leaf against α -glucosidase and pancreatic lipase reached 191.37 ± 14.46 and 775 ± 68.60 μg/mL, respectively. Meanwhile, it also promoted glucose consumption in the HepG2 cells model. Subsequently, four α -glucosidase ligands and four pancreatic lipase ligands were identified through bio-affinity ultrafiltration combined with liquid-chromatography-mass spectrometry (UF-HPLC–MS/MS). In vitro inhibition assays confirmed the inhibitory potential of these representative ligands. Molecular docking suggested that these possible ligands could be inserted into the active pocket of the target enzyme through multiple intermolecular forces. Our findings indicated that the polyphenols in mulberry leaves may contribute significantly to their hypoglycemic and hypolipidemic properties. Furthermore, our study provides valuable insights for the screening of active ingredients in other medicinal and edible plants, which could help in the development of new therapies for diabetes and obesity.
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CERAMICS INTERNATIONAL
·
2025
·
IF 5.6
Dazhao Liu,Ting Fang,Kaixuan Gui,Miao Wang,Wengang Zhang,Dongyang Zhang
In this study, α-Al 2 O 3 platelets with controlled size and uniform thickness were successfully synthesized at 900 °C using a molten salt synthesis method. The effects of calcination temperature, holding time, molten salt and additive concentration on the morphology, particle size, and phase composition of α-Al 2 O 3 were systematically investigated. The results revealed that calcination temperature significantly influenced the particle size and platelet morphology, with higher temperatures leading to larger particles and more irregular structures. Prolonged calcination time increased both the diameter and thickness of the platelets. The content of molten salts and Na 3 PO 4 was critical in regulating platelet uniformity and growth. An appropriate amount of TiCl 4 facilitated the formation of a flake-like structure. This work provides valuable insights into the tailored synthesis of α-Al 2 O 3 platelets, offering a cost-effective method for producing advanced materials with potential applications in various fields.
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FOOD CHEMISTRY
·
2025
·
IF 9.8
Lichun Liu,Ziye Zhang,Wei Yang,Fatih Özogul,Zhenxing Li,Hong Lin
α-dicarbonyl compounds are widely present in foods, while their modification effects on food allergens are unclear. This work was conducted to investigate the effects of α-dicarbonyl compounds (glyoxal (GO), methylglyoxal (MGO) and 2, 3-butanedione (BU)) on the digestive stability and intestinal flora of shrimp tropomyosin (TM). As the results, higher α-dicarbonyl concentration, longer time and higher temperature could exacerbate the modification of TM. Compared with TM, GO and MGO significantly reduced the digestive resistance of TM and the IgG-binding capacity of AGEs-TM digests. Although in vitro digestion reduced IgE-binding capacity of TM/AGEs-TM, in vivo evidence (intestinal biopsy and gut microbiota) suggested that they still elicits immune responses. In addition, AGEs-TM could reduce the richness and diversity of intestinal flora, increase the abundance of inflammation-related flora and flora imbalance index, which could provide a new insight into the effects of AGEs on the digestive stability of shrimp tropomyosin and intestinal microbiota.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Hong-Jiao Zhang,Li-Wei He,Ke-Min Dong,Song-Yuan Li,Can Luo,Jia-Qi Han,Rui Li
The development of simple, selective and sensitive analytical methods to effectively monitor α-glucosidase (α-Glu) and discover its new inhibitors is very critical for the diagnosis and treatment of diabetes. Herein, an autofluorescence-free sensing method based on β-cyclodextrin (β-CD) functionalized persistent luminescence nanoparticle (PLNP) is established for the detection of α-Glu activity and its inhibitor. The p-nitrophenol (PNP) produced by the hydrolysis of substrate p-nitrobenzene-α-D-glucopyranoside (PNPG) by α-Glu in the system can be selectively captured by the β-CD on the nanoprobe to form adducts through the host guest effect between them, resulting in persistent luminescence quenching due to electron transfer (ET) effect. The proposed method provides a good linear relationship within the range of 5–80 U/L and a low detection limit of 0.9 U/L, and can detect α-Glu in human serum with the recovery rate of 95.4–103.3 %. Furthermore, the detection system can measure the inhibitory ability of acarbose with an IC 50 value of 349 μM, proving its good potential for application.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2024
·
IF 7.7
Qi Meng,Xiao Tan,Bi Wu,Siyan Zhang,Yuangang Zu,Shougang Jiang
The decoctions of sunflower ( Helianthus annuus L. HAL) stalk pith have been used to treat advanced cancer, and polysaccharide of sunflower stalk pith (HSPP) was key ingredient of the decoctions. To forage specially structured HSPP with anti-tumor effects and to uncover its mechanisms of anticancer activity, syngeneic mouse model of lung carcinoma metastasis was established and the HSPP was found to contain long-chain fatty acid. Encouragingly, the mean survival of the polysaccharide group (47.3 ± 12.8 d) and its sub-fractions group HSPP-4 (50.7 ± 13.0 d) was significantly increased compared with control group (38.7 ± 12.7 d) or positive control group (41.8 ± 13.4 d), ( n = 20, P < 0.01 vs. the control group or positive control group). Furthermore, the HSPP exerted inhibitory effects on the tumor cells' metastasis. Eventually, it is postulated that the polysaccharide could inhibit tumor proliferation and metastasis by reduction of TNF-α from the macrophage.
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PROGRESS IN ORGANIC COATINGS
·
2024
·
IF 6.5
Tao Li,Zhiping Zhu,ShangMing Zhou,Huo Yang
2D nanofillers can effectively improve coatings' barrier and anti-corrosion properties but are less compatible with coatings and prone to agglomeration. In this paper, glucose-derived phosphorus-doped carbon dots (P-CDs) were prepared as dispersants, and functionalized α-zirconium phosphate (α-ZrP) was prepared by combining with α-ZrP through π-π interactions, which showed good dispersion and compatibility in waterborne epoxy (WEP). The corresponding modified WEP coating is prepared on Q235 substrates through the scrape coating method. The effect of fillers on the physical and chemical properties of the coating was studied by electrochemical impedance spectroscopy (EIS), neutral salt spray test, and immersion test. The results of the electrochemical test show that the |Z| 0.01 Hz value of the coating prepared by P-CDs@ZrP as the filler after soaking in 3.5 wt% NaCl solution for 30 d is three orders of magnitude higher than that of the WEP coating, and the corrosion resistance is greatly improved. The salt spray and soaking tests prove that the prepared P-CDs@ZrP coating has excellent barrier properties and corrosion resistance. Besides, P-CDs can also act as an effective passivation agent, which could adsorb on the surface of the Q235 substrate and form a passivation film, preventing further corrosion of the substrate. This study provides a new way to enhance the corrosion resistance of the coating.
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