Frontiers in Microbiology
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2019
·
IF 4.259
Zhang Jian-Mei,Sun Yin-Shuang,Zhao Li-Qin,Chen Tian-Tian,Fan Mei-Na,Jiao Hong-Chao,Zhao Jing-Peng,Wang Xiao-Juan,Li Fu-Chang,Li Hai-Fang,Lin Hai
The impact of gut microbiota and its metabolites on fat metabolism have been widely reported in human and animals. However, the critical mediators and the signal transductions are not well demonstrated. As ovipara, chicken represents a specific case in lipid metabolism that liver is the main site of lipid synthesis. The aim of this study is to elucidate the linkage of gut microbiota and fat synthesis in broiler chickens. The broilers were subjected to dietary treatments of combined probiotics (Animal bifidobacterium: 4 × 108 cfu/kg; Lactobacillus plantarum: 2 × 108 cfu/kg; Enterococcus faecalis: 2 × 108 cfu/kg; Clostridium butyrate: 2 × 108 cfu/kg, PB) and guar gum (1 g/kg, GG), respectively. Results showed that dietary supplementation of PB and GG changed the cecal microbiota diversity, altered short chain fatty acids (SCFAs) contents, and suppressed lipogenesis. In intestinal epithelial cells (IECs), SCFAs (acetate, propionate, and butyrate) up-regulated the expression of glucagon-like peptide-1 (GLP-1) via mitogen-activated protein kinase (MAPK) pathways, mainly via the phospho - extracellular regulated protein kinase (ERK) and phospho-p38 mitogen activated protein kinase (p38 MAPK) pathways. GLP-1 suppressed lipid accumulation in primary hepatocytes with the involvement of (AMP)-activated protein kinase/Acetyl CoA carboxylase (AMPK/ACC) signaling. In conclusion, the result suggests that SCFAs-induced GLP-1 secretion via MAPK pathway, which links the regulation of gut microbiota on hepatic lipogenesis in chickens.
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RSC Advances
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2020
·Vol. 10
·期 2
·1015-1020
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IF 3.119
Xing Wei,Yu-Jia Wang,Tie-Qiang Ren,Hai-Yan Wang,Min Wei
Hydrothermal synthesis is a typical method for the preparation of TS-1. In the current study, agitation was introduced during the crystallisation stage, and nano-sized TS-1 with little anatase TiO2 was successfully synthesised in a short time (1–8 h). Furthermore, under rotational crystallisation conditions, a series of TS-1 samples was prepared with different crystallisation times, and the products obtained were investigated as catalysts for the oxidative desulfurisation of thiophene from a model fuel. All samples were characterised utilising X-ray diffraction (XRD), scanning electron microscopy (SEM), N2-adsorption, ultraviolet-visible (UV-Vis) spectroscopy and Fourier-transform infrared (FT-IR) spectroscopy techniques. It was found that under rotational crystallisation conditions, nano-sized TS-1 could be synthesised in a short time that had improved efficiency for the oxidative desulfurisation reaction of thiophene in comparison with TS-1 synthesised by static crystallisation for 24 h.
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JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
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2019
·Vol. 94
·期 6
·1951-1957
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IF 2.659
Zhimin Ou,Jiaying Pan,Lan Tang,Hanbing Shi
BACKGROUND Magnetic nanoparticle immobilized enzymes are attractive owing to their potential applications in many biological fields. When an alternating magnetic field is applied, the magnetic moments of the magnetic nanoparticles are apt to align in the direction of the field, and the magnetic nanoparticles experience force in the direction of the field. Thus, the magnetic nanoparticles behave like microscopic stirrers. RESULTS In this study, (R)- N -(1-phenylethyl)acetamide was prepared continuously using immobilized lipase in an alternating magnetic field. The effect of several factors on the acylation reaction were investigated. A kinetic model of the continuous acylation reaction process also was developed. The conversion of 1-phenylethanamine reached a maximum value of 41.8% with lipase concentration 75 mg mL −1 (dry weight/reaction volume), substrate concentration 500 mmol L −1 and flow rate 25 μL min −1 The enantiomeric excess of (R)- N -(1-phenylethyl)acetamide was > 98.4%. The kinetic constants of the continuous reduction were r m = 2.25 × 10 −2 mmol L –1 min −1 , k cat = 6 × 10 −4 mmol L –1 min −1 , K A = 0.75 mmol L –1 , K B = 4.36 × 10 −3 , K QA = 2.43 × 10 −2 and K BQA = 6.2 × 10 −4 mmol L –1 . The kinetic model was in good agreement with the experimental data obtained during continuous acylation reaction. CONCLUSION The results revealed that the inhibition of the acylation reaction at high substrate concentrations was eliminated during the continuous reaction. Compared with the batch acylation reaction, the continuous acylation reaction showed significantly improved lipase catalyst efficiency. © 2019 Society of Chemical Industry
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CATALYSIS LETTERS
·
2019
·Vol. 149
·期 4
·974-984
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IF 2.372
Zhang Xiaotong,He Ning,Liu Chunyan,Guo Hongchen
Highly dispersed Pt–Cu alloy nanoparticles encapsulated in silicalite-1 were prepared by one-pot method. Taking propane dehydrogenation as a probe reaction, it was found that anti-coke ability was improved by encapsulation. Through H 2 -TPR, CO adsorption DRIFT spectra and X-ray Photoelectron Spectroscopy experiment, enhanced anti-coke ability was ascribed to the electronic environment change of encapsulated metal. And it was further confirmed by in situ coking experiment. Owning to limited acidity, metal encapsulated by silicalite-1 molecular sieve exhibited better anti-coke ability and higher propylene selectivity than that by stronger acidic ZSM-5 molecular sieve. Moreover it was found that the combination of Cu and Pt made better catalytic performance and accelerated dehydrogenation more effectively compared with monometallic catalysts. Therefore, Pt–Cu alloy nanoparticles encapsulated in silicalite-1 molecular sieve core–shell material was a potential coke-resistant catalyst for alkane dehydrogenation reaction. Graphical Abstract
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Molecular Medicine Reports
·
2019
·Vol. 19
·期 5
·3658-3666
·
IF 1.851
Jin Xu,Yin Ying,Gaoyun Xiong,Liqin Lai,Qingliang Wang,Yue Yang
Nasopharyngeal carcinoma (NPC) is a type of cancer originating in the nasopharynx. There are no NPC‑specific treatments available at present. Serpin peptidase inhibitor clade C member 1 (SERPINC1) serves roles in anticoagulation and anti‑inflammation. The aim of the present study was to investigate the role of SERPINC1 in the proliferation and apoptosis of NPC cells. Tumor and adjacent healthy tissue samples were collected from patients with NPC. Additionally, the SERPINC1 gene was silenced in the HNE3 cell line using short interfering RNA targeted against SERPINC1 (SERPINC1‑siRNA). Cell viability was determined via a Cell Counting Kit‑8 assay; furthermore, proliferation and apoptosis were investigated via flow cytometry. Western blotting and reverse transcription‑quantitative polymerase chain reaction analysis were performed to determine the expression levels of protein and mRNA. It was revealed that the expression levels of SERPINC1 mRNA and protein were increased in NPC tumor tissues compared with in adjacent healthy tissues. The expression of SERPINC1 mRNA and protein in HNE3 cells decreased following SERPINC1‑siRNA transfection. Furthermore, knockdown of SERPINC1 promoted apoptosis and inhibited proliferation. It was also demonstrated that silencing SERPINC1 upregulated the expression of B‑cell lymphoma-2 (Bcl‑2)‑associated X protein and p53 mRNA and protein, and downregulated that of Bcl‑2, survivin and cyclin D1. Downregulation of SERPINC1 reduced the phosphorylation of phosphatidylinositol 3‑kinase (PI3K), protein kinase B (Akt) and mammalian target of rapamycin (mTOR). Thus, SERPINC1 knockdown may promote the apoptosis of HNE3 cells and inhibit proliferation via the suppression of the PI3K/Akt/mTOR signaling pathway.
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BIOSENSORS & BIOELECTRONICS
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2019
·
IF 9.518
Xiaoxue Fu,Junlin He,Chengli Zhang,Jun Chen,Yilin Wen,Jia li,Weiran Mao,Hangtian Zhong,Jiahao Wu,Xingduo Ji,Chao Yu
In this work, an aptamer was used as the target capturing agent and a trimetallic signal amplification strategy based on [email protected] and AuPtRu NPs was demonstrated for the sensitive detection of TSP-1. Herein, the synthesized AuPtRu nanocomposite (AuPtRu NPs) not only acts as the catalyst for catalyzing hydrogen peroxide but also acts as a nanocarrier for capturing the -NH 2 termination single strand DNA (S1) to obtain the signal probe (SP, AuPtRu nanocomposite/S1). Then, SP was efficiently linked into TSP-1 aptamers with the addition of complementary linking strands to form M1 (SP/aptamer). The [email protected] nanocomposites were obtained by in situ reduction and used as GCE electrode modification materials. The -NH 2 -modified capture probe (CP) DNA was immobilized on the surface of [email protected] nanocomposites for hybridizing SP. In the presence of the target TSP-1, the aptamer recognizes the target and binds strongly so that SP is released from the prepared M1 and then hybridized with CP. When the detection solution contains an electrochemical matrix of H 2 O 2 , AuPtRu NPs can oxidize H 2 O 2 to obtain an enhanced signal. Furthermore, the proposed aptasensor has a very low LOD of 0.13 fg mL −1 TSP-1 in the detection range of 1 fg mL −1 to 10 ng mL −1 . Moreover, the proposed platform also has application implications for other potential targets.
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AMB Express
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2019
·Vol. 9
·期 1
·1-9
·
IF 2.226
Zhao Shuxue,Hu Chunhui,Guo Lizhong,Li Kuiran,Yu Hao
A 3-hydroxypyridine degrading bacterium, designated strain DW-1, was isolated from petroleum contaminated soil in Liao River China. 16S rRNA-based phylogenetic analysis indicates that strain DW-1 belongs to genus Agrobacterium. The optimal cultivation temperature and pH for strain DW-1 with 3-hydroxypyridine were 30 °C and 8.0, respectively. Under optimal conditions, strain DW-1 could completely degrade up to 1500 mg/L of 3-hydroxypyridine in 66 h. The 3-hydroxypyridine degradation pathway of strain DW-1 was suggested by HPLC and LC–MS analysis. The first reaction of 3-hydroxypyridine degradation in strain DW-1 was α-hydroxylation so that the major metabolite 2,5-dihydroxypyridine was produced, and then 2,5-dihydroxypyridine was transformed by a Fe2+-dependent dioxygenase to form N-formylmaleamic acid. N-Formylmaleamic acid will be transformed to maleic acid and fumaric acid through maleamic acid. This is the first report of the 3-hydroxypyridine degradation pathway and the utilization of 3-hydroxypyridine by a Agrobacterium sp. It may be potentially used for the bioremediation of environments polluted with 3-hydroxypyridine.
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SENSORS AND ACTUATORS B-CHEMICAL
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2019
·
IF 6.393
Ensheng Xu,Haitang Yang,Lina Wu,Jin Chen,Wei Wei,Yong Liu,Songqin Liu
Since the expression of poly(ADP-ribose) polymerase-1 (PARP-1) was significantly up-regulated in several primary malignancies, it was important to construct a sensitive and rapid PARP-1 assay for early diagnosis of cancer. When PARP-1 was activated by active dsDNA, it generates a number of negatively charged poly(ADP-ribose) (PAR) polymers, which can be readily detected. Here, we developed a label-free electrochemical (EC) biosensor for PARP-1 activity based on the electrostatic attraction between negatively charged PAR and positively charged amino-functioned mesoporous silica films (NH 2 -MSFs). Negatively charged PAR was absorbed on the NH 2 -MSFs surface and suppressed the electrochemical signal as the PAR prevented the electroactive probe reaching to the ITO electrode. The electrochemical signal for PAR linearly decreased from 0.01 U to 1.2 U with a detection limit of 0.005 U, which is comparable to the PARP-1 biosensors reported before. The strategy has been used to evaluate PARP-1 inhibitors and to detect PARP-1 activity in human serum and real cancer cells lysate with satisfactory results, indicating that it was a promising candidate for point-of-care diagnostics.
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MICROPOROUS AND MESOPOROUS MATERIALS
·
2019
·
IF 4.182
Ruyue Niu,Pengcheng Liu,Wei Li,Shuang Wang,Jinping Li
0.123%Pd encapsulated in nanosized silicalite-1 (MFI) zeolite catalysts has been prepared by direct hydrothermal conditions, and its catalytic performance at the low concentration methane combustion was studied. The temperatures for methane ignition ( T 10% ), the half-conversion temperature ( T 50% ) and the total conversion temperature ( T 90% ) over Pd/S-1-in (in-situ method) catalyst are 261, 329 and 382 °C, respectively, which are lower than the corresponding temperatures of Pd/S-1-im (incipient impregnation method) catalyst. Moreover, the properties are further enhanced by introducing CeO 2 into the Pd/S-1-in catalyst. The conversion rate decreased slightly from 91 to 85% when the reaction is carried out at 450 °C for 100 h at the gas hourly space velocities (GHSV) of 112500 mL g −1 h −1 . The XPS analysis showed that Pd clusters were encapsulated within the porous rather than on the surface of the crystal by in-situ method. The HRTEM studies demonstrated that the Pd particle encapsulated within Silicalite-1 zeolites are well dispersed and evenly distributed throughout the Silicalite-1 zeolites. The average size of in situ Pd particle encapsulated in Silicalite-1 zeolite is 1.7 nm, which is much smaller than the impregnated Pd/S-1-im catalyst (2.8 nm).
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CHEMICAL ENGINEERING JOURNAL
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2019
·
IF 8.355
Yang Xiang,Xiaohui Duan,Longbao Feng,Shuqi Jiang,Li Deng,Jun Shen,Yijun Yang,Rui Guo
The successful treatment of gliomas relies on two important factors, namely therapeutic genes/drugs and efficient delivery vehicles to cross the blood-brain barrier. In this work, a new gene vector was constructed based on polycaprolactone (PCL), glutathione (reduced; GSH)-sensitive polyethylenimine (PEI-SS) and polyethylene glycol (PEG), and modified with a cell-penetrating peptide (tLyp-1); this yielded tLyp-1-conjugated GSH-sensitive biodegradable micelles (PPPt) that mediated enhanced tumour necrosis factor (TNF)-related apoptosis-inducing ligand (pUNO1-hTRAILa) and curcumin (CUR) co-delivery to gliomas. The sensitisation function of CUR was used to explore whether CUR and genes have a synergistic antitumour effect. The results showed that the drug loading efficiency of PPPt was about 8.6%. The PPPt possessed good cytocompatibility and blood compatibility when the concentration was less than 0.06 mg/mL. PPPt strongly condensed pUNO1-hTRAILa to form a nanocomplex and efficiently transfected C6 cells. The drug-release behaviour of PPPt/CUR/pUNO1-hTRAILa was reduction-sensitive and the cellular uptake results indicate that modification of tLyp-1 promoted the internalisation of micelles. The PPPt/CUR/pUNO1-hTRAILa complexes exhibited much better in vitro and in vivo antitumour effects than micelles loaded with CUR or pUNO1-hTRAILa alone. We also used magnetic resonance imaging for in vivo monitoring of the transcranial drug delivery and therapeutic effect of the tumour on rats bearing in situ glioma.
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APPLIED CLAY SCIENCE
·
2019
·
IF 3.89
Shangying Li,Hongping He,Qi Tao,Yunfei Xi,Aiqing Chen,Shichao Ji,Chaoqun Zhang,Yiping Yang,Jianxi Zhu
In this study, beidellite , paragonite and other 2:1 type layered aluminosilicates with high layer charges (2:1 type LA-HLC) were synthesized under hydrothermal conditions by using boehmite as a precursor. The concentrations of dissolved Al from boehmite precursor had important effects on the mineral components of the hydrothermal products and the layer charge density of the resultant minerals. A combination of 2:1 type LA-HLC, paragonite, and minor beidellite formed at a relatively high concentration of dissolved Al, while beidellite with a small amount of 2:1 type LA-HLC was produced at a low concentration of dissolved Al. The resultant 2:1 type LA-HLC exhibited poor swelling ability when treated with ethylene glycol. A solid-state transformation mechanism was proposed for the transformation based on the HRTEM images and EDS analyses, which showed that lattice fringes representing (001) faces of 2:1 type layered aluminosilicates (2:1 type LA) were parallel to the (020) direction of boehmite. The successful transformation of boehmite into 2:1 type LA provides insights to well understand the formation of 2:1 type LA in geological processes and offer a facile preparation approach for the synthesis of 2:1 type LA with different layer charges.
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FREE RADICAL BIOLOGY AND MEDICINE
·
2019
·
IF 5.657
Xueming Cao,Na Zhu,Li Li,Yuwei Zhang,Yan Chen,Jing Zhang,Jiang Li,Chuanyu Gao
Aims Y-box protein 1 (YB1) is a key regulator of inflammatory mediators . However, the roles of YB1 in oxidized low-density lipoprotein (ox-LDL)-induced macrophage inflammation and lipid uptake remain less understood. Thus, we explored the roles of YB1 in ox-LDL-induced macrophage inflammation and lipid uptake and its underlying molecular mechanisms. Methods An ox-LDL-induced atherosclerosis (AS) model was used in this study. Western blotting , RT-PCR, immunofluorescence, ELISA, dil-ox-LDL staining, a dual-luciferase reporter assay, RNA-binding protein immunoprecipitation (RIP) and in vivo experiments were used to detect each target. Results ox-LDL downregulates YB1 expression in THP-1-derived macrophages and human monocyte-derived macrophages (hMDMs) via the NF-κB pathway. Downregulation of YB1 is facilitated by lipid uptake in macrophages, and CD36 is involved in this process. Furthermore, YB1 suppresses CD36 protein levels by directly binding to the coding sequence of the CD36 gene to promote CD36 mRNA decay but does not affect its mRNA transcription. Additionally, YB1 knockdown enhances the inflammatory response and lipid deposition via the NF-κB pathway in vivo. Conclusion ox-LDL decreases YB1 expression in macrophages, resulting in enhanced inflammatory responses by affecting NF-κB and facilitating lipid uptake by promoting scavenger receptor CD36 mRNA decay.
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