JOURNAL OF APPLIED POLYMER SCIENCE
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2022
·Vol. 139
·期 22
·52268
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IF 3.057
Zhixiang Cui,Yue Fan,Xiaolong Liu,Junhui Si,Qianting Wang
Nowadays, the environmental problems caused by industrial oily wastewater and offshore oil leakage are becoming more and more serious. Here, the 3-D porous polyurethane/deacetylated acetate cellulose (TPU/d-CA) composite foam with outstanding mechanical property and oil/water separation performance was successfully prepared. The morphology, wettability, mechanical properties, and oil/water separation performances had been systematically studied. The results showed that TPU/d-CA composite foam could be used as water and oil removing material, respectively, for a series of oil/water mixture only under gravity force, due to its lipophilicity and hydrophilicity. It has high-separation flux, which can reach up to 43,000 L/m 2 ·h and 85,000 L/m 2 ·h for water and oil, respectively and all the separation efficiency exceeds 98%. It also can be used as adsorbent material to absorb various oils, and the absorption capacity for carbon tetrachloride reached up to 34 g/g. Moreover, it can selectively, rapidly, and continuously separate oil or water by assisted peristaltic pump. After 15 cycle times, its separation efficiency is still as high as 98%. It has excellent comprehensive mechanical properties and antifouling ability. These results indicated that 3-D TPU/d-CA composite foam has broadly application prospects in oil/water separation field.
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ACS Sustainable Chemistry & Engineering
·
2022
·
IF 9.224
Yu Peng,Luyao Jiang,Junhua Di,Cuiluan Ma,Qi Li,Yucai He
Biobased 2,5-bis(hydroxymethyl)furan is a versatile building block for the production of drugs, polymers, crown ethers, bioactive compounds, and value-added intermediates. To explore a more efficient route to synthesize 2,5-bis(hydroxymethyl)furan, a tandem transformation by quick dehydration with a deep eutectic solvent betaine:benzenesulfonic acid (Bet:BSA) and mild bioreduction with recombinant Escherichia coli (E. coli) DCF was developed for converting d-fructose into 2,5-bis(hydroxymethyl)furan. The 5-hydroxymethylfurfural yield reached 62.3 ± 1.6% (based on d-fructose) in Bet:BSA at 120 °C after a short time (2 min). d-Fructose-valorized 5-hydroxymethylfurfural was biologically transformed to 2,5-bis(hydroxymethyl)furan by newly constructed recombinant E. coli DCF containing reductase and formate dehydrogenase at 40 °C and pH 7.5 after 48 h, with a yield of 99.6 ± 0.1% (based on 5-hydroxymethylfurfural) in Bet:BSA-water containing the cosubstrate HCOONa (5-HMF-to-HCOONa molar ratio, 1:2.5). Meanwhile, 150 mM commercial 5-hydroxymethylfurfural was transformed into 2,5-bis(hydroxymethyl)furan in a yield of 94.4 ± 0.9%. An efficient hybrid process was constructed to valorize d-fructose to 2,5-bis(hydroxymethyl)furan in Bet:BSA-water, which has good industrial application potential.
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BIOSENSORS & BIOELECTRONICS
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2022
·
IF 12.545
Fei Chen,Daian Chen,Ting Deng,Jishan Li
Pathogenic bacteria are a major cause of foodborne diseases, which not only seriously threaten human safety but also cause significant losses for the national economy. Therefore, it is very important and urgent to develop a method for the detection of pathogenic bacteria with high accuracy, high sensitivity, and easy interpretation for use in food safety and medical hygiene. Herein, based upon the sensitive color changes induced by the dispersion and aggregation states of gold nanoparticles (AuNPs), a point-of-care (POCT) colorimetric assay was constructed for the rapid and sensitive visual detection of pathogenic bacteria. The POCT visual sensing system is composed of two individual elements: (1) an alkaline phosphatase/graphene oxide (GO@PEI-ALP) nanoconjugate that can release free ALP molecules in the presence of pathogenic bacteria; (2) D-glucose-6-phosphate (pGlu) and 3-aminobenzene boric acid (AMBA)-functionalized AuNPs (pGlu/AMBA-AuNPs) that are cross-linked upon the digestion of pGlu by free ALP molecules, resulting in a significant color change. Under optimized conditions, the detection limit of this sensing system for target bacteria was as low as 24 CFU mL −1 and was successfully applied to complex real samples. This proposed rapid colorimetric assay has high sensitivity, accuracy, and practicability with an intuitive signal and is expected to provide new inspiration for the detection of pathogenic bacteria.
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JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
·
2022
·Vol. 103
·期 2
·779-791
·
IF 4.125
Huanhuan Teng,Yi Zhang,Chuanshan Jin,Tongsheng Wang,Shengzhuo Huang,Lei Li,Songzi Xie,Deling Wu,Fengqing Xu
BACKGROUND Polygonatum cyrtonema Hua is cultivated for its edible and medical value. The steam-processed rhizome of P. cyrtonema is the main form for daily consumption and it has been used traditionally in tonics for treating various age-related disorders. The aim of our study was to compare the physicochemical properties and antioxidant activity of polysaccharides respectively extracted from crude P. Cyrtonema (PCPC), and steam-processed P. cyrtonema (PCPS), and to explore a possible underlying antioxidant mechanism. RESULTS The PCPC with a molecular weight of 4.35 × 10 3 Da mainly consisted of fructose and trace amounts of glucose, whereas PCPS with 4.24 × 10 4 Da was composed of fructose, arabinose, glucose, xylose, mannose, galacturonic acid and glucuronic acid. The PCPC had a triple-helical conformation whereas PCPS was a random coil. Both exhibited free radicals- scavenging activity in vitro . In a mouse model of oxidative damage, PCPC or PCPS treatment significantly reversed histopathological alterations, reactive oxygen species (ROS) accumulation and the reduction of antioxidant enzyme activity. They both also promoted Nrf2 nuclear transport by decreasing Keap-1 expression and increasing HO-1 expression. Both in vitro and in vivo , PCPS exhibited more potent antioxidant activity than PCPC. CONCLUSION Overall, the results suggest that PCPS has a stronger effect on the prevention of oxidative damage by activating Nrf2/HO-1 antioxidant signaling. This study demonstrates the role of steam-processed P. cyrtonema rhizome and provides valuable perspective for PCPS as a functional agent. © 2022 Society of Chemical Industry.
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TALANTA
·
2021
·
IF 6.057
Haizhi Zhang,Huanyu Yang,Pei Liu,Xinguang Qin,Gang Liu
A rapid colorimetric method for detecting sodium benzoate in food products was established based on the d -amino acid oxidase (DAAO) and 2D metal organic framework (2D MOF) nanosheets mediated cascade enzyme reactions. Firstly, the synthesized 2D MOF nanosheets served as high efficient nanozyme with outstanding peroxidase-like catalytic activity and catalyzed the color reaction between H 2 O 2 and 3, 3’, 5, 5′- tetramethylbenzidine. Secondly, sodium benzoate as a competitive inhibitor of DAAO, could influence the production of H 2 O 2 in DAAO mediated oxidation reaction. After a combination of those two reactions, this colorimetric quantitative method was constructed and validated for sodium benzoate determination with wide linear range (2.0–200.0 μM), low limit of detection (2.0 μM), high accuracy (recovery rate in 95.80–108.00%) and satisfied selectivity . Lastly, this method was utilized to analyze sodium benzoate concentration in juice, wine and vinegar by naked eyes.
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JOURNAL OF MATERIALS SCIENCE
·
2021
·Vol. 56
·期 31
·17584-17600
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IF 4.22
Huo Kaili,Wang Jin,Zhuang Tao,Zhao Yuqiang,Gao Boru,Dou Mengmeng,Wang Xiaoyue,Fu Yao,Wang Dongying,Ci Lin
The immobilization of catalysts hinders the application of nanosized photocatalysts in wastewater treatment. In this study, a simple method for the preparation of flexible composite non-woven catalysts with a good spectral response is presented. Firstly, the porous g-C 3 N 4 nanosheets containing nitrogen defects (DCN) and sodium alginate (SA) were blended to prepare the casting solution and then coated on the surface of polyester and viscose blend non-woven fabric (NW). Finally, the robust three-dimensional composite non-woven catalyst (DCN@SA/NW) was constructed by cross-linking with CaCl 2 . The as-prepared samples were characterized by different physicochemical techniques including SEM, EDS, FTIR, Raman, XRD, XPS, and TGA. The photocatalytic degradation of dyes and antibiotics and self-cleaning and antibacterial properties of the catalyst were investigated. The optimal DCN@SA/NW formulation was 2.0 wt.% DCN and 2.0 wt.% SA. The DCN@SA/NW composite efficiently degraded rhodamine (RhB) and tetracycline hydrochloride (TC). Moreover, the synthesized non-woven composite catalyst was easily recycled and remained stable, indicating its promising potential in wastewater treatment. The DCN@SA/NW also exhibited outstanding self-cleaning and antibacterial performance. This study provides an interesting idea for the research and development of immobilized photocatalysts and has great potential in future practical photocatalytic applications.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2021
·
IF 4.932
Lu Wang,Xiaohui Wang,Lina Kong,Shuyuan Wang,Kai Huang,Jingjing Wu,Changyuan Wang,Huijun Sun,Kexin Liu,Qiang Meng
Acute liver failure (ALF) is a dramatic liver disease characterized by large areas of inflammation. However, there are no available effective targeted drugs for ALF treatment. In the study, serum biochemical index and H&E were used to explore the amelioration of the liver histopathological changes. The oxidative stress kits, quantitative real-time PCR, western blot, immunohistochemistry, immunofluorescence staining, reactive oxygen species (ROS), and siRNA were used to elucidate the mechanisms underlying isoliquiritigenin (ISL) protection. The results showed that ISL significantly improved the liver pathological changes. Furthermore, ISL reduced oxidative stress by altering the expression of PGC-1α, Nrf2, HO-1, NQO1, Keap1, GCLC, and GCLM in damaged hepatocytes. Moreover, the levels of inflammation-related genes including NLRP3 inflammasome, IL-1β, IL-6, TNF-α, iNOS, and Mip-2 were repressed by ISL. In addition, ISL alleviated LPS/D-GalN-induced hepatocytes apoptosis by increasing the Bcl-2/Bax ratio and suppressing the expression of cleaved caspase-3. Further in vivo and in vitro evidence proved the involvement of the PGC-1α/Nrf2 signaling pathway in ISL protection. In conclusion, ISL improves the ability of anti-oxidative stress, alleviates inflammatory reaction, apoptosis, and inhibits NLRP3 inflammasome to protect lipopolysaccharide/D-galactosamine (LPS/D-GalN)-induced ALF through activating the PGC-1α/Nrf2 pathway, which provides the possibility for the treatment of ALF.
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FOOD CHEMISTRY
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2021
·
IF 7.514
Dan Bu,Zong-cai Tu,Hui Wang,Yue-ming Hu,Qing Sun,Guang-xian Liu
The mechanism of d -allose in reducing the allergenicity and digestibility of ultrasound-pretreated α-lactalbumin (α-LA) was studied. The intensity reduction and peak red shift occurred in fluorescence spectra of glycated samples. Enzyme-linked immunosorbent assay and basophil degranulation analysis showed that d -allose significantly reduced the allergenicity of α-LA, and ultrasound-pretreated α-LA showed the lowest allergenicity after glycation. Compared with α-LA, the degree of hydrolysis decreased in glycated samples. Size-exclusion high-performance liquid chromatography showed that the glycated α-LA was resistant to digestive enzymes. The glycated sites and average degree of substitution per peptide molecule were determined using LC Orbitrap MS/MS. These results suggested that the masking of linear allergenic epitopes by glycation could reduce the allergenicity. Therefore, the combination of ultrasound pretreatment and glycation is a potential method to reduce protein allergenicity in food processing and provides a useful approach for the application of rare sugars in food processing.
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JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 56
·期 1
·731-745
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IF 3.553
Wang Jingguang,Gao Huichang,Hu Yang,Zhang Naiyue,Zhou Wuyi,Wang Chaoyang,Binks Bernard P.,Yang Zhuohong
Abstract Biocompatible and bioactive scaffolds with multiscale porosity, shape memory effect and drug delivery performance are highly promising in bone tissue engineering applications within the approach of minimally invasive surgical implantation. In this study, we report an effective method to fabricate multiscale porous bioactive (MPB) scaffolds with shape memory effect by freeze drying of 3D printed high internal phase Pickering emulsion (Pickering HIPE) templates stabilized by hydrophobically modified hydroxyapatite and silica nanoparticles. MPB scaffolds containing a shape memory polymer matrix of poly(D,L-lactide-co-trimethylene carbonate) display high porosity and fully interconnected filament networks with multiscale pore structures. The in vitro biomineralization study verifies that MPB scaffolds are bioactive with the apatite formation ability. The thermal property testing shows that the glass transition temperature of MPB scaffolds is easily tuned to fall between 45.9 and 49.1 °C by varying the HIPE composition. Moreover, MPB scaffolds exhibit observable shape memory properties with fast shape recovery. The antibacterial drug enrofloxacin can be efficiently loaded into MPB scaffolds and then released in a sustained manner displaying effective antimicrobial effect. Furthermore, cell culture assays confirm that MPB scaffolds could sustain the cell growth, proliferation and osteogenic differentiation, indicating the cytocompatibility and osteoinduction activity of MPB scaffolds. Therefore, combining freeze drying with 3D printing of Pickering HIPE templates paves the way towards MPB scaffolds with shape memory effect, which are promising as minimally invasive implantation scaffolds in bone tissue engineering applications. Graphic Abstract
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JOURNAL OF NANOBIOTECHNOLOGY
·
2020
·Vol. 18
·期 1
·1-15
·
IF 6.518
Zhang Man,Ye Lili,Huang Hao,Cheng Dandan,Liu Kaixin,Wu Wenbo,Shen Fukui,Jiang Zhihong,Hou Yuanyuan,Bai Gang
Background Nanoparticle-based pulmonary drug delivery systems are commonly developed and applied for drug-targeted delivery. They exhibit significant advantages compared to traditional pulmonary drug delivery systems. However, developing the formulation of each drug is a time-consuming and laborious task.Results In this study, a universal lung-targeting nanoparticle was designed and constructed. The self-assembled micelles were composed of a platycodon secondary saponin, 3-O-β-d-glucopyranosyl platycodigenin 682 (GP-682), based on its specific amphiphilic structure. The GP-682 micelles exhibited a relatively stable zeta potential with a particle size between 60 and 90 nm, and the critical micelle concentration (CMC) value was approximately 42.3 μg/mL. Preincubation of GP-682 micelles markedly enhanced their cell membrane permeability and improved drug uptake in vitro. The results were visualized using fluorescent dye tracing, transmission electron microscopy (TEM) observations and the lactate dehydrogenase (LDH) release assay. The obtained benefits enhanced the distribution of levofloxacin (Lev) in mouse lung tissue and reduced antibiotics overdosing. The acute lung injury mouse model induced by the Pseudomonas aeruginosa PA 14 strain demonstrated that preinjection of GP-682 micelles before antibiotic administration resulted in a higher survival rate and anti-infective efficacy in vivo. It also caused reductions in pulmonary injury, bacterial invasion and cytokine expression compared with treatment with Lev alone.Conclusion sGP-682 micelles are another nanoparticle-based pulmonary drug delivery system and provide a new lung-targeting therapy option.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2021
·
IF 6.953
Junxin Zhao,Shichang Liu,Yifang Gao,Maomao Ma,Xianghui Yan,Ding Cheng,Dongman Wan,Zheling Zeng,Ping Yu,Deming Gong
Enzymatic degradation has become the most promising approach to degrading organic ester compounds. In this study, Bacillus licheniformis NCU CS-5 was isolated from the spoilage of Cinnamomum camphora seed kernel, and its extracellular lipase was purified, with a specific activity of 192.98 U/mg. The lipase was found to be a trimeric protein as it showed a single band of 27 kDa in SDS-PAGE and 81 kDa in Native-PAGE. It was active in a wide range of temperatures (5–55 °C) and pH values (6.0–9.0), and the optimal temperature and pH value were 40 °C and 8.0, respectively. The enzyme was active in the presence of various organic solvents, metal ions, inhibitors and surfactants. Both crude and purified lipase retained more than 80% activity after 5 h in the presence of commercial detergents, suggesting its great application potential in detergent industry. The highest activity was found to be towards medium- and long-chain fatty acids (C 6 -C 18 ). Peptide mass spectrometric analysis of the purified lipase showed similarity to the lipase family of B. licheniformis . Furthermore, it degraded more than 90% 2,4-D butyl ester to its hydrolysate 2,4-D within 24 h, indicating that the novel lipase may be applied to degrade organic ester pesticides.
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NATURAL PRODUCT RESEARCH
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2020
·
IF 2.158
Xiaomei Li,Xiaoman Li,Jing Zhu,Haoxin Wang,Chunhua Lu
One new carbamothioic S-acid derivative (1) and five known kigamicin derivatives (2–6) were isolated from the fermentation extract of Amycolatopsis alba DSM 44262Δabm9 elicited by N-acetyl-D-glucosamine. HPLC-DAD-UV analyses indicated that the DSM 44262Δabm9 strain did not produce these metabolites originally and the production of 1–6 was induced by adding 25 mM N-acetyl-D-glucosamine in the culture medium. The structures of 1–6 were identified on the basis of NMR spectroscopic data and high-resolution ESIMS. These results highlight that addition of N-acetyl-D-glucosamine in the microbial culture medium could activate cryptic gene expression, induce and increase the production of new or known secondary metabolites.
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