ANALYTICAL CHEMISTRY
·
2017
·
IF 6.32
Yanjun Wang,Luzhu Yang,Baoxin Li,Chaoyong James Yang,Yan Jin
Detection of telomerase activity at the single-cell level is one of the central challenges in cancer diagnostics and therapy. Herein, we describe a facile and reliable point-of-care testing (POCT) strategy for detection of telomerase activity via a portable pressure meter. Telomerase primer (TS) was immobilized onto the surface of magnetic beads (MBs), and then was elongated to a long single-stranded DNA by telomerase. The elongated (TTAGGG)n repeat unit hybridized with several short PtNP-functionalized complementary DNA (PtNPs-cDNA), which specifically enriched PtNPs onto the surfaces of magnetic beads (MBs), which were separated using a magnet. Then, nanoparticle-catalyzed gas-generation reaction converted telomerase activity into significant change in gas pressure. Because of the self-amplification of telomerase and enrichment by magnetic separation, the diluted telomerase equivalent to a single HeLa cell was facilely detected. More importantly, the telomerase in the lysate of 1 HeLa cell can be reliably detected by monitoring change in gas pressure, indicating that it is feasible and possible to study differences between individual cells. The difference in relative activity between different kinds of cancer cells was easily and sensitively studied. Study of inhibition of telomerase activity demonstrated that our method has great potential in screening of telomerase-targeted antitumor drugs as well as in clinical diagnosis.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF SEPARATION SCIENCE
·
2017
·Vol. 41
·期 2
·440-448
·
IF 2.557
Mengmeng Chen,Guohua Zhu,Shanshan Wang,Kezhi Jiang,Jiaxiang Xu,Jinsong Liu,Jianxiong Jiang
The chromatographic separation of several isomeric anilines is a challenging issue. Herein, a simple method for the simultaneous determination of four groups of isomeric primary aromatic amines, including chloroanilines, methylanilines, methoxylanilines, and dimethylanilines, was presented. In this method, all of the 15 primary aromatic amines were easily transformed into the corresponding imine derivative by treatment with benzaldehyde under mild conditions. The formed isomeric imine derivatives were completely separated on a commercial capillary gas chromatography column. The effects of several derivatization parameters were investigated and optimized. Linearity in the optimized method ranged from 0.050 to 50 μg/mL with the squared correlation coefficients ( R 2 ) between 0.9981 and 0.9999. Reasonable reproducibility was obtained, with the intraday relative standard deviation ( N = 5) ranging from 0.89 to 4.57% and interday relative standard deviation ranging from 2.26 to 7.69% at the concentration of 5.0 μg/mL. The developed method has been successfully applied to determine these isomeric aromatic amines in real samples.
✓ 已验证
DOI
PubMed
高影响因子
CHEMICAL ENGINEERING SCIENCE
·
2017
·
IF 2.895
Shao-Bin Zhang,Xue-Hui Ge,Yu-Hao Geng,Guang-Sheng Luo,Jian Chen,Jian-Hong Xu
In this article, gas-liquid-liquid emulsion drops were firstly generated by one-step method in a coaxial microfluidic device. Based on that, the effects of several parameters on the morphology transition from core-shell to Janus droplets was studied systematically. By tuning flow rates, interfacial tensions and viscosity, we can change the size and morphology of droplets. Furthermore, non-spherical microparticles were obtained by using photo-responsive reagent through solidification. The developed approach offers a novel and simple strategy on the synthesis of Janus emulsions and anisotropic microparticles with controlled morphologies.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL THERMODYNAMICS
·
2016
·
IF 2.196
Shurui Xu,Shuanshi Fan,Haiyuan Yao,Yanhong Wang,Xuemei Lang,Pingping Lv,Songtian Fang
In this paper, the three-phase coexistence points are generated for multicomponent gas hydrate in methanol (MeOH) solution for (0.05, 0.10, 0.15, and 0.35) mass fraction and ethylene glycol (EG) solution for (0.05, 0.10, 0.15, 0.35, 0.40 and 0.55) mass fraction. The phase equilibrium curves of different system were obtained by an isochoric pressure-search method on high pressure apparatus. The phase equilibrium regions of multicomponent gas hydrate were measured using the same composition of natural gas distributed in the South China Sea. And the different concentration solutions were prepared based formation water. The experimental data were measured in a wide range temperature from 267.74 to 298.53 K and a wide range pressure from 4.22 MPa to 34.72 MPa. The results showed that the hydrate phase equilibrium curves shifted to the inhibition region in accordance with the increased inhibitor concentration. In addition, the equilibrium temperature would decrease about 2.7 K when the concentration of MeOH increased 0.05 mass fraction. Besides, the suppression temperature was 1.25 K with the 0.05 mass fraction increase of EG concentration in the range of 0.05 mass fraction to 0.15 mass fraction. While in high EG concentration region, the suppression temperature was 3.3 K with the same increase of EG concentration (0.05 mass fraction).
✓ 已验证
产品关联
DOI
高影响因子
CERAMICS INTERNATIONAL
·
2016
·
IF 2.758
Lu-ming Huang,Rong-jun Liu,Yan-fei Wang,Chang-rui Zhang,Xian-hai Long,Ying-bin Cao
The dense Silicon Carbide (SiC) ceramics are fabricated by means of gel-casting and gas silicon infiltration (GSI) using carbon black and α-SiC as raw materials. We have successfully introduced a new initiator AIBA which is very suitable to aqueous gel-casting system containing carbon black, overcoming the problems posed by the conventionally used initiator. We have investigated the influences of the monomer acrylamide (AM) content, the ratio of the monomer to crosslinking agent AM/MBAM content, the particle size distribution and the solid content on the mechanical and structural properties of samples. The result show that, the linear shrinkage of the green body can be reduced to 1.0% and its bending strength can reach 59.2 MPa at the optimized gel-casting process that has an AM content of 25 wt%, an AM to MBAM ratio of 12, a SiC particle distribution of 3/2 and a solid content of 60 vol%. After the GSI process, the bending strength and elastic modulus of the final products from such green bodies can reach 245 MPa and 220 GPa respectively. The study highlights that the combined application of the gel-casting and the GSI processes can produce high-quality silicon carbide ceramics that are suitable in the space optical applications.
✓ 已验证
DOI
高影响因子
Materials Research Express
·
2017
·Vol. 4
·期 9
·095022
·
IF 1.068
Haojie Duan,Lei Yan,Yue He,Haiying Li,Li Liu,Yali Cheng,Liting Du
In2O3 nanofibers (NFs) and toruloid nanotubes (TNTs) were fabricated by conventional electrospinning process and their sensing properties toward H2S gas were researched. Thermogravimetric analysis, x-ray diffractometry, energy dispersive spectroscopy and scanning electron microscopy were carefully used to investigate the structures, chemical compositions and morphologies of these samples. The novel structure (TNTs) first used nonpolar mineral oil as the control agent of morphology, and the principle of forming toruloid nanotubes was studied. Phase separation plays a critical role in the process of forming toruloid nanotubes structure automatically. The new application of phase separation provided a simple and effective approach for controlling structure. The research indicated that thin-walled toruloid nanotubes showed higher responses toward H2S compared to nanofibers or thick-walled toruloid nanotubes. Additionally, their excellent selectivity can be attributed to the special gas-sensing mechanism of In2O3 for H2S.
✓ 已验证
产品关联
DOI
高影响因子
RSC Advances
·
2017
·Vol. 7
·期 11
·6179-6186
·
IF 3.108
Huaqi Zhan,Yongjun Hu,Pengchao Wang,Jiaxin Chen
Morpholine (NH(CH2CH2)2O) is a typical six-membered aliphatic heterocyclic compound. Herein, infrared plus vacuum ultraviolet (IR/VUV) single photon “soft” ionization spectroscopy was employed to study the structures of neutral morpholine and its monohydrated clusters. Theoretical calculations revealed that the structures containing equatorial-chair and axial-chair conformations were the most stable conformers in the gas phase, and this was confirmed by IR spectral analysis. Analysis of the observed and calculated spectra of the monohydrated clusters suggested that multiple conformers may co-exist in the molecular beam, and that the water molecule acts as a hydrogen donor. In the most stable structure, the hydrogen atom of the water molecule is bound to the NH group of the equatorial-chair conformer of morpholine. Moreover, the water molecules simultaneously serve as hydrogen bond donors for the NH group and interact with the CH group weakly. It is suggested that the weak intermolecular CH⋯O interaction is also responsible for the molecular stability.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2017
·
IF 6.035
Xingyu Wu,Zhizhang Tian,Shaofei Wang,Dongdong Peng,Leixin Yang,Yingzhen Wu,Qingping Xin,Hong Wu,Zhongyi Jiang
Polymers of intrinsic microporosity (PIMs) exhibit the inherent superior permeability over traditional commercial polymers, however, the relatively low selectivity restrict their broad application in gas separation. In this study, novel kinds of mixed matrix membranes (MMMs) combined covalent organic frameworks (COFs) and PIMs were prepared and utilized for improving the selectivity of carbon capture process. Covalent organic framework (SNW-1) was synthesized via Schiff base chemistry and incorporated into polymer of intrinsic microporosity (PIM-1) to prepare MMMs. Pure gas permeation tests and mixed gases separation performances of the MMMs were conducted. Owing to its proper porosity characteristics and good compatibility with PIM-1 matrix, the incorporation of SNW-1 significantly intensified the diffusion process of CO 2 within the membrane. Compared with pure PIM-1 membrane, the prepared MMMs showed an increment of 27.4% and 37.6% in selectivity of CO 2 /CH 4 and CO 2 /N 2 along with an enhancement of 116% in CO 2 permeability. The strategy of incorporating COFs into polymers of intrinsic microporosity may hold promising potential in preparing high performance MMMs for gas separation.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2017
·
IF 6.035
Mingmin Jia,Yi Feng,Shichang Liu,Jianhao Qiu,Jianfeng Yao
UiO-66-NH 2 were either intercalated into graphene oxide (GO) layers (GOU@S) or covered by GO layers (GO/U@S) to form UiO-66-NH 2 /GO composite membrane . Results show that different synthesis methods would lead to different membrane morphology, thickness and gas separation performance. For GOU@S membrane, UiO-66-NH 2 was well-dispersed and intercalated into GO layer, whereas two continuous layers (GO and UiO-66 layer) with an obvious boundary were observed for GO/U@S. Due to the morphology and structure difference, better hydrogen separation performance was achieved for GOU@S membrane compared to GO/U@S membrane and pristine membrane due to the close interfacial contact between UiO-66-NH 2 and GO, which arises from the hydrogen bonding formed between -NH 2 group in UiO-66-NH 2 and -OH group in GO and the electrostatic force between UiO-66-NH 2 and GO layers. The ideal selectivities of H 2 /N 2 and H 2 /CO 2 were increased to 9.75 and 6.35, respectively, for the GOU@S membrane with a H 2 permeance of 3.9 × 10 −8 mol m −2 s −1 Pa −1 .
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2016
·
IF 4.758
Ye Zhu,Zhixuan Cheng,Qun Xiang,Xikun Chen,Jiaqiang Xu
Mesoporous TiO 2 -SiO 2 functionalized with p-hexa-fluoroisopropanol aniline (HFIP) was designed and synthesized to achieve highly sensitive and selective sensing toward nerve agent simulant dimethyl methylphosphonate (DMMP) due to a hydrogen bonding between target DMMP and TiO 2 -SiO 2 -HFIP. The sensing experiments were conducted by coating compounds onto quartz crystal microbalance (QCM) transducers. It was found that the observed frequency shifts gradually and turned saturated in the range from 10 to 60 ppm. Gas sensing test results revealed that sensors had enhanced sensitivity due to the decoration of HFIP on TiO 2 -SiO 2 in a ppb level. Furthermore, when the doping of HFIP reached 20 wt%, the sensors achieved the best sensing performance. In conclusion, the QCM sensor coated with mesoporous TiO 2 -SiO 2 -HFIP exhibited fast response, excellent reversibility and high selectivity to nerve agent stimulant DMMP vapor, implying that mesoporous TiO 2 -SiO 2 -HFIP may be a good candidate for sensing DMMP.
✓ 已验证
产品关联
DOI
高影响因子
Journal of Environmental Chemical Engineering
·
2024
·
IF 7.4
Min Chen,Jiangzhou Luo,Jun Hu,Yuxuan Zhao,Honglei Ling,Xueping Zong,Song Xue
The network polyimide (PI) precursor is a promising candidate to fabricate the advanced carbon molecular sieve (CMS) membrane since its robust crosslinked structure could prevent pore structure collapse during CMS formation. Exploring the relationship between network PI precursor structure and CMS membrane is highly significant. Herein, a novel brominated network PI precursor was fabricated via in-situ cross-linking technique firstly, and then the thermally induced bromination/debromination process was applied to tune the microporosity and gas transport properties of network PI precursor and its derived CMS membranes. After the bromination/debromination and carbonization at 550 °C, the resultant CMS membrane exhibited both high gas permeability and gas selectivities compared to its un-brominated network PI precursor derived CMS membrane. This is due to the bromination/debromination and carbonization enable the CMS membrane possesses large d -spacing values (11.06 Å), high BET surface areas (538.57 m 2 /g), and high percentage (∼71 %) of ultra-micropores. For instance, 6F-TA/TA-Br-550/30 membrane exhibits great CO 2 /CH 4 separation ability, with a CO 2 permeability of 6300 Barrer and a CO 2 /CH 4 selectivity of 46.11, which is suppressed the latest 2019 trade-off curves. We anticipate that the present bromination/debromination and carbonization could provide a fresh perspective on the rational design of network PI precursor and its derived CMS membrane with excellent gas separation performance.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS
·
2024
·Vol. 24
·期 23
·7623
·
IF 3.4
Yinying Liao,Lu Qiu,Yunfei Ouyang,Dayang Feng,Shiyi Huang,Zhaoyang Zhang,Xinyao Xie,Junwei Ke,Tianhao Liu,Xiangxiang Chen,Hongshan Bi,Weiran Zuo
This study investigates the ethanol gas-sensing mechanisms of ZnO nanocrystals with distinct morphologies, synthesized via a hydrothermal method using various alkali sources. Significant differences in the gas-sensing performance and morphology of ZnO samples synthesized with ammonium carbonate (Na2CO3), hexamethylenetetramine (HMTA), ammonia solution (NH3·H2O), and sodium hydroxide (NaOH) were observed. ZnO were confirmed to be impurity-free through XRD analysis, and their morphological features were characterized by SEM. TEM, XPS, and FTIR were employed to further analyze the crystal structure and binding energy of ZnO. To elucidate the underlying mechanisms, density functional theory (DFT) calculations combined with electron depletion layer theory were applied to assess charge transfer processes and identify the most sensitive ZnO crystal planes for ethanol detection. Experimental gas-sensing tests, conducted across 5–1000 ppm ethanol concentrations within a 150–350 °C range, showed that ZnO prepared with Na2CO3, HMTA, and NaOH was responsive at high ethanol concentrations as low as 100 °C, while ZnO synthesized with ammonia required 250 °C to exhibit sensitivity. All ZnO samples demonstrated excellent recovery at low concentrations at 250 °C. By integrating experimental findings with theoretical insights, this study provides a comprehensive understanding of ethanol gas-sensing mechanisms in ZnO, highlighting the role of crystal plane engineering and charge transfer dynamics as critical factors influencing gas response.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）