ANALYTICA CHIMICA ACTA
·
2025
·
IF 6
Ruiying Lin,Zhangying Jiang,Jinyong Lin,Feifei Tong,Yangmin Wu,Jinquan Hong,Sufang Qiu,Qiong Wu
Background In recent years, significant progress has been made in the treatment of nasopharyngeal carcinoma (NPC). The application of immunotherapy, especially the use of Programmed Cell Death Protein 1 inhibitors, has demonstrated excellent therapeutic effects. However, inconsistent immunotherapy outcomes due to individual differences in patients remain a major challenge, making the search for specific biomarkers to screen for the population who may benefit from immunotherapy a priority. Surface-enhanced Raman spectroscopy (SERS) has shown great potential as a highly sensitive and specific optical analytical tool for identifying and monitoring tumor-related markers in NPC. Results In this study, plasma samples from Nasopharyngeal cancer patients before and after immunotherapy, along with those from healthy volunteers, were tested using label-free SERS combined with plasmapheresis. Especially, the components with varying molecular weight sizes were analyzed via the separation process, thereby preventing the potential loss of diagnostic information that could result from competitive adsorption. Subsequently, a robust machine learning algorithm based on principal component analysis and linear discriminant analysis (PCA-LDA) was used to extract features from plasma SERS data and establish an effective discriminant model. The results showed that in the upper plasma layer, the most optimal discrimination was found between normal patients and those post-treatment, with sensitivity and specificity of 88.5% and 92.3%, respectively. In the lower plasma layer, the most optimal discrimination was found between the pre-treatment and post-treatment patients, with sensitivity and specificity of 84.6% and 80.8%, respectively. Significance and novelty Plasmapheresis can reveal latent diagnostic information in the plasma and holds promise for treating NPC, the screening of the population who may benefit from immunotherapy, and the postoperative evaluation of immunotherapy. Further exploration of the feasibility of using SERS to detect tumor markers in the population who may benefit from immunotherapy for NPC will help improve the therapeutic efficacy, optimize clinical practice, and promote the development of individualized treatment strategies.
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高影响因子
JOURNAL OF HAZARDOUS MATERIALS
·
2025
·
IF 11.3
Yansheng Liu,Yunjie Zhan,Guofu Wang,Xiaobo Jia,Jin Zhou,Hongqi Li,Haixin Chang,Zhicheng Jin,Kang Li,Zhaoxu Li
This study proposes a strategy for the highly sensitive detection of polystyrene nanoplastics (PS NPs) with varying particle sizes. Ag nanoarrays (AgNAs) with different inter-column spacings and heights are fabricated via thermal deposition of Ag in anodized aluminum oxide (AAO) templates. The size-matching effects between PS NPs and the parameters of the AgNAs (inter-column spacing and height) are investigated. Utilizing this size-matching effect, the AgNAs substrate enables sensitive detection of PS NPs with particle sizes of 130 nm, 180 nm, and 230 nm, with limits of detection (LODs) of 10 μg/mL. In real water samples (river water, rainwater, and tap water), the AgNAs substrate also demonstrates good performance, achieving a LOD of 10 μg/mL for detecting 130 nm PS NPs. Additionally, toluene is used to remove PS NPs from the AgNAs surface, allowing the substrate to be reused across multiple cycles. After at least 30 detection cycles, the surface-enhanced Raman scattering (SERS) performance of the AgNAs shows no significant decline, with a relative standard deviation (RSD) of 6.8 %. The AgNAs exhibit excellent stability and reusability in detecting PS NPs, indicating strong potential for practical applications.
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高影响因子
MATERIALS SCIENCE FORUM
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2020
·
IF 0
Wan Ying Li,Jia Hao Li,Jing Hong Ma,Jing Hua Gong
Surface-enhanced Raman scattering (SERS) spectroscopy technology has broad application prospects in food safety, environmental monitoring, surface science and material analysis because of the characteristics of ultra-high sensitivity and non-destructive testing. However, there are still some challenges in the preparation of SERS substrates. As SERS substrates, the common colloidal noble metal nanoparticles usually show low storage stability and poor repeatability of analytical results. In order to overcome these limitations, a coaxial microfluidic spinning device was designed to prepare flexible SERS substrates in this paper. Based on the microfluidic spinning and subsequent in-situ reduction reaction of AgNO3, novel gel fibers uniformly loaded with AgNPs were successfully prepared. The effects of the concentration of AgNO3 solution and UV irradiation duration on the formation of AgNPs were investigated. Transmission electron microscopy (TEM) showed that the average particle size was about 2.7 nm. The gel fibers loaded with AgNPs were used as SERS substrates to detect 4-mercaptobenzoic acid (4-MBA), which showed obvious Raman enhancement effect and good repeatability. The relative standard deviation of 10 test results was 4.75%, and the detection line range was 10-14-10-5 mol·L-1.
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ANALYTICAL CHEMISTRY
·
2022
·
IF 8.008
Shu Li,Xiaochen Lv,Qing Yang,Shaoying Zhang,Jie Su,Shi-Bo Cheng,Yongchao Lai,Jing Chen,Jinhua Zhan
The core of the surface-enhanced Raman spectroscopy (SERS)-based techniques for dynamic monitoring is to realize rapid and reversible adsorption. Herein, the integration technology of electro-enhanced adsorption, solid-phase microextraction, and surface-enhanced Raman spectroscopy (EE–SPME–SERS) was developed to obtain sensitive, ultrafast, and reversible SERS response toward in situ monitoring of pharmaceuticals and personal care products (PPCPs). In the EE–SPME–SERS method, a roughened Ag fiber with Au modification (r-Ag/Au fiber) was used as the SERS substrate, SPME sorbent, and working electrode. The r-Ag/Au fiber displayed good SERS sensitivity, ultrahigh photostability, and adsorption properties. The adsorption efficiency of benzidine was 76 times accelerated in EE–SPME–SERS compared to that in static adsorption. The whole process of “sampling and detection” in EE–SPME–SERS can be finished within 1 s. Reversible adsorption and desorption can be achieved in situ by switching the direction of electric field, and the regeneration process takes only a few minutes. Simulated release of benzidine from household wastewater was in situ and dynamically monitored using this strategy. EE–SPME–SERS was proved universal for ionized PPCPs and can detect multicomponents simultaneously. In addition, EE–SPME–SERS showed very good analytical properties. Great potential of EE–SPME–SERS can be expected in environmental monitoring.
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高影响因子
Journal of Food Measurement and Characterization
·
2022
·Vol. 16
·期 3
·2314-2322
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IF 3.006
Chen Ying,Cao Jilin,Wei Hengyong,Wu Zhengang,Wei Yingna,Wang Xuepei,Pei Yuan
Silver nanoparticles (Ag NPs) were decorated on the electrospun polyvinyl alcohol (PVA)/polyethyleneimine (PEI) nanofibers by electrostatic adsorption in order to prepare Ag@PVA/PEI SERS substrate. The morphology and chemical structure analysis show that the metal Ag NPs are decorated on the PVA/PEI nanofibers surface uniformly. The lowest distinguishable level of Rhodamine 6G (R6G) is 10 −6 M of Ag@PVA/PEI nanofibers SERS substrate. It exhibits remarkable enhancement effect (EF = 1.4 × 10 5 ), good reproducibility (RSD = 9.5%). By the investigations of Raman spectra analysis, the Ag@PVA/PEI nanofibers substrate presented high sensitivity in the detection of enrofloxacin. Therefore, it facilitates a simple, direct, in-situ method for enrofloxacin residue detection on an irregular prawn surface. Graphical abstract
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高影响因子
Nanoscale
·
2014
·Vol. 6
·期 23
·14514-14522
·
IF 6.739
Weiwei Zhang,Yunqing Wang,Xiuyan Sun,Wenhai Wang,Lingxin Chen
Recently surface-enhanced Raman scattering (SERS) imaging guided theranostic nanoplatforms have attracted considerable attention. Herein, we developed novel yolk–shell gold nanorod@void@mesoporous titania nanoparticles (AuNR@void@mTiO2 NPs) for simultaneous SERS imaging and chemo-photothermal therapy. Our work showed three highlighted features: first, we proposed a facile and versatile “up to down” SERS labeling strategy for the drug delivery system, in which “empty carriers” were pre-synthesized, followed by co-loading of Raman reporters on AuNR and anti-cancer drug doxorubicin (DOX) in mTiO2 in sequence. The acquired SERS signal was strong enough for tracking NPs at both living cells and mice levels. Second, we selected mTiO2 as a novel drug loading material instead of the widely used mesoporous silica (mSiO2). The mTiO2 shared satisfactory drug loading and release behavior as mSiO2 but it was chemically inert. This property not only provided a facile way to form a yolk–shell structure but also rendered it with superior structural stability in a biological system. Third, the near infrared (NIR) light absorbing property of the AuNR SERS substrate was also explored for drug release regulation and photothermal treatment. Significantly greater MCF-7 cell killing was observed when treated together with DOX-loaded NPs and NIR laser irradiation, attributable to the synergistic chemo-thermal therapeutic effect. Our results indicated the established SERS labeled yolk–shell NP as a promising theranostic platform and suggested its potential in vivo applications.
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高影响因子
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
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2021
·
IF 3.935
Liping Wang,Jinling Mo,Yuhong Xia,Tian Lu,Yang Jin,Yan Peng,Liying Zhang,Yulin Tang,Shuhu Du
The evaluation of true penicillin allergy is significant to reduce its occurrence and the overdiagnosis before anti-infective treatment. However, the currently available methods with high specificity still face the problem of low sensitivity, thereby easily leading to false negatives. Herein, an alkyne responsive surface-enhanced Raman scattering (SERS) immunosensor is reported for ultrasensitive detection of penicillin allergen penicilloyl protein (P-protein) by using Au-Ag alloy nanoparticles@(antibody + alkyne probe) (as SERS immunoprobe) together with Ag nanofilm modified by antibody (as SERS capture substrate). The SERS immunoassay integrates the interference-free Raman response of high wavenumber region (2212 cm −1 ) and specific capture antibody with high affinity to selectively recognize P-protein from complicated sample. Meanwhile, the target-induced near-field coupling effect between localized surface plasmon resonances of individual SERS immunoprobe and capture substrate enables the detection of P-protein as low as pg/mL level, and the limit of detection can reach 0.329 pg/mL that is about 6 orders of magnitude lower than the limit defined protein residue (causing penicillin allergy). With the ultrasensitivity and specific selectivity , the proposed SERS immunoassay platform can precisely evaluate the content of P-protein in blood sample or penicillin drugs. It will be a potential tool to monitor allergic reaction to penicillin and better understand the mechanism of penicillin hypersensitivity.
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高影响因子
FOOD CONTROL
·
2021
·
IF 5.548
Alberta Osei Barimah,Zhiming Guo,Akwasi A. Agyekum,Chuang Guo,Ping Chen,Hesham R. El-Seedi,Xiaobo Zou,Quansheng Chen
Arsenic (As) is one of the toxic, persistent, and lethal heavy metalloids and requires rapid, less costly, and sensitive detection methods. This study proposed a label-free cuprous oxide/silver (Cu 2 O/Ag) surface-enhanced Raman scattering (SERS) nanoprobe to detect total As in tea. Different total As spiked tea concentrations were mixed with the Cu 2 O/Ag SERS nanoprobe for the SERS detection. Quantitative models were established for predicting the total As in tea by comparatively applying chemometric algorithms. Amongst the algorithms, competitive adaptive reweighted sampling partial least squares (CARS-PLS) optimized the most effective spectral variables to predict the total As in tea efficiently. The CARS-PLS gave the highest correlation coefficient value (R p = 0.9935), very low root means square error (RMSEP = 0.0496 μg g −1 ) in the prediction set and recorded the highest RPD value of 8.819. The proposed nanoprobe achieved a lower detection limit (0.00561 μg g −1 ), excellent selectivity, satisfactory reproducibility, and stability. No significant difference was recorded when the performance of the Cu 2 O/Ag total As SERS sensor was compared with the inductively coupled plasma mass spectrometry (ICP-MS) method. Therefore, this developed Cu 2 O/Ag coupled chemometrics SERS sensing method could be used to efficiently determine, quantify, and predict total As in tea to promote monitoring of heavy metal contaminants.
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高影响因子
Theranostics
·
2022
·
IF 11.6
Bohan Yin,Qin Zhang,Xinyue Xia,Chuanqi Li,Willis Kwun Hei Ho,Jiaxiang Yan,Yingying Huang,Honglian Wu,Pui Wang,Changqing Yi,Jianhua Hao,Jianfang Wang,Honglin Chen,Siu Hong Dexter Wong,Mo Yang
Background: CRISPR-Cas12a has been integrated with nanomaterial-based optical techniques, such as surface-enhanced Raman scattering (SERS), to formulate a powerful amplification-free nucleic acid detection system. However, nanomaterials impose steric hindrance to limit the accessibility of CRISPR-Cas12a to the narrow gaps (SERS hot spots) among nanoparticles (NPs) for producing a significant change in signals after nucleic acid detection. Methods: To overcome this restriction, we specifically design chimeric DNA/RNA hairpins (displacers) that can be destabilized by activated CRISPR-Cas12a in the presence of target DNA, liberating excessive RNA that can disintegrate a core-satellite nanocluster via toehold-mediated strand displacement for orchestrating a promising “on-off” nucleic acid biosensor. The core-satellite nanocluster comprises a large gold nanoparticle (AuNP) core surrounded by small AuNPs with Raman tags via DNA hybridization as an ultrabright Raman reporter, and its disassembly leads to a drastic decrease of SERS intensity as signal readouts. We further introduce a magnetic core to the large AuNPs that can facilitate their separation from the disassembled nanostructures to suppress the background for improving detection sensitivity. Results: As a proof-of-concept study, our findings showed that the application of displacers was more effective in decreasing the SERS intensity of the system and attained a better limit of detection (LOD, 10 aM) than that by directly using activated CRISPR-Cas12a, with high selectivity and stability for nucleic acid detection. Introducing magnetic-responsive functionality to our system further improves the LOD to 1 aM. Conclusion: Our work not only offers a platform to sensitively and selectively probe nucleic acids without pre-amplification but also provides new insights into the design of the CRISPR-Cas12a/SERS integrated system to resolve the steric hindrance of nanomaterials for constructing biosensors.
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高影响因子
ANALYTICAL CHEMISTRY
·
2025
·
IF 6.7
Jiafeng Wang,Daijie Xie,Xiaozhang Qu,Tian Li,Xiuping Meng,Zhimin Zhang,Yongdong Jin,Guohua Qi
Mitochondrial fusion protein 2 (Mfn2) as a molecular gatekeeper in mitochondria plays a momentous role in regulating mitochondrial morphology and function to further influence cell behavior. However, ultrasensitive and in situ measurement of low-abundance Mfn2 at the single-cell level remains a challenge, especially during external stress. Herein, an ultrasensitive and “turn-on”-type immunoassay platform for analysis of Mfn2 expression at the single-cell level during the dental pulp stem cell (DPSC) differentiation process was exploited through surface-enhanced Raman spectroscopy (SERS) as a readout modality. In this system, the detection sensitivity of Mfn2 is markedly enhanced through the generation of gap-plasmon “hot spots” caused by the biocoded SERS nanoprobes consisting of AuNPs and AgNPs following the affinity binding of Mfn2. The developed SERS immunoassay exhibited a wide linear relationship for Mfn2, ranging from 0.5 ng/mL to 5 μg/mL. Notably, the biocoded plasmonic nanoprobes reacting with Mfn2 can self-assemble to form an SERS immunoassay biosensor within single cells, which enabled sensitive measurement of Mfn2 expressions during the DPSC differentiation process evoked by electric-impulse stimulation (EIS). Mechanistically, the Mfn2 expression within DPSCs showed palpable downregulation during the EIS-induced differentiation process. The work revealed at the single-cell level a previously unknown role of Mfn2 in DPSC differentiation during the EIS process. The developed SERS immunoassay sensing platform is promising for the early diagnosis of diseases associated with Mfn2.
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高影响因子
Journal of Environmental Chemical Engineering
·
2025
·Vol. 13
·期 6
·120102
·
IF 7.2
Ziyu Zhou,Sheng Zhang,Dieyu Song,Jingyi Cao,Yuqin Qiu,Changguo Xue,Huajie Xu,Jinfei Mei
Contact-electro-catalysis (CEC) has performed outstandingly in the organic degradation, precious metals reduction, and clean energy conversion. However, this interaction of ultrasonic and particles has not yet obtained a general change law. Herein, an acoustic-solid interaction is proposed and validated, elucidating the critical role of ultrasonic-inert particle synergy in CEC. Using methyl orange (MO) solution as dye probe and inert PTFE as the target particles, the particles enhance local acoustic pressure and modulate the standing wave acoustic field, breaking the cavitation threshold. Compared with the case without adding particles, the amplitude of the positive and negative acoustic wave increased by 16.22 and 25.53 times, respectively, when PTFE particles is added. Under ultrasonic-PTFE particle synergy, the kinetic constant for MO solution degradation reaches 2.489 h −1 ·(g·L −1 ). This value is more than 9 times higher than this without particle system. Furthermore, the PTFE-Ag composites prepared by CEC-in-situ reduction, in which the Ag-based materials demonstrate high crystallinity and uniform distribution characteristics. Surface-enhanced Raman scattering (SERS) technology based on PTFE-Ag enables highly sensitive detection of ciprofloxacin (10 −3 mol/L). This is attributed to the presence of Ag element can significantly enhance the SERS signal intensity, validating its potential applications in environmental restoration and pollutant detection.
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高影响因子
Food Bioscience
·
2022
·
IF 5.318
Siqian Shi,Hang Yu,Fangwei Yang,Weirong Yao,Yunfei Xie
Nitroimidazoles are a family of veterinary drugs containing a 5-nitroimidazole ring structure, which are commonly used antibiotics. In view of their potential genotoxicity and possible coexistence in actual samples, it is necessary to establish a rapid and effective method for the simultaneous determination of multiple nitroimidazoles. In this study, a rapid thin-layer chromatography combined with surface-enhanced Raman spectroscopy (TLC-SER) detection method for 14 nitroimidazole compounds has been established. The theoretical Raman spectra of the nitroimidazoles were calculated using the B3LYP/6-311+G(d) basis set in density functional theory (DFT) and the theoretical Raman spectra of TNZ , NBZ, ONZ , SCZ, DMZ , MNI, 2-HMZ, 4-HMZ, CMNI, IPZ , MNZ-OH and HMDMZ have been obtained for the first time. SERS detection was carried out on a gold nanoparticles (AuNPs) substrate, which exhibited strong SERS signals and a limit of detection limit of 0.1 mg/L. By optimizing the conditions used for the developing agent in TLC, 12 nitroimidazoles were successfully separated, while MNZ and RNZ still could not be separated. When combined with the chemometrics analysis method, the TLC-SERS method can realize the simultaneous detection of 14 nitroimidazoles. Actual pork samples were spiked with nitroimidazoles with a recovery rate of 80%–110% and RSD of 0.74%–16.70%, which showed that SERS and TLC-SERS methods are also effective for detection in actual samples. The detection time of this method is < 10 min, which is suitable for the rapid detection of nitroimidazoles, especially for the rapid qualitative identification of nitroimidazoles in situ.
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