WASTE MANAGEMENT
·
2026
·
IF 7.3
Tianhang Tang,Chenlu Yang,Huikang Song,Hao Liu,Hao Shi,Yaji Huang
A highly efficient and environmentally friendly plasma co-melting vitrification process was developed. • The mechanisms of heavy metal fixation and migration during the plasma melting process were investigated. • Changes in the valence states and forms of various elements during the melting process were studied. Hazardous fly ash (HFA) contains large amounts of toxic heavy metals and poses significant potential risks to the environment. In this study, HFA was co-melted with hazardous bottom ash (HBA) and waste glass (WG) in a plasma system to investigate its vitrification behavior, heavy metal migration, and solidification mechanisms. The results indicate high vitrification efficiency (99.5 %) and ash solidification rate (97.1 %). The original multi-phase crystalline structure transformed into a structure dominated by an amorphous silicate network following plasma melting and rapid cooling. The surface became smoother and denser, and heavy metals were distributed uniformly, indicating enhanced physical encapsulation capacity and a more homogeneous structure. Although a considerable amount of heavy metals remained in the melt product (GP), the leaching concentrations of all detected heavy metals were significantly reduced. BCR results further indicate improved long-term stability of heavy metals. Additionally, the solidification mechanism was elucidated, involving a synergistic process of glass network encapsulation, fixation in iron-rich crystalline phases, and volatilization-redistribution-residual stabilization. As, Pb, and Cu are primarily immobilized in the glass phase, while Cr and Ni are preferentially stabilized in iron-rich spinel/ferrite-like phases; Zn and Mn exhibit dual-host behavior between the glass and crystalline phases. The results demonstrate that plasma co-melting vitrification is an effective method for the detoxification, stabilization, and resource utilization of hazardous incineration residues.
✓ 已验证
产品关联
DOI
高影响因子
SENSORS AND ACTUATORS B-CHEMICAL
·
2021
·
IF 7.46
Jianguo Chang,Nailong Gao,Peng Dai,Ziming Zhu,Hui You,Wei Han,Lu Li
The powerful immunoassay has attracted increasing attention for detecting trace substances. Herein, based on the co-coupling of phenylboronic acid and Protein A for oriented antibodies immobilization, we proposed a polymeric device-based fluorescence immunoassay with enhanced assay performance for Alpha-fetoprotein (AFP) analysis. Unlike traditional physical absorption or chemical covalent conjugation to immobilize antibodies, in this immunoassay, the bioreactivity of AFP antibody was maximumly obtained owing to the oriented immobilization manner. The package density of AFP antibody on the polymethylmethacrylate (PMMA) surface is significantly improved because of the co-coupling case associated distance effect. In addition, the non-specific absorption is greatly inhibited with the combined using of zwitterionic 2-methacryloyloxyethyl phosphorylcholine (MPC) and bovine serum albumin (BSA). These integrated features facilitate the output of strong fluorescence in the presence of AFP but emit very weak background in the absence of AFP. After a typical sandwich immunoreaction, the recorded fluorescence intensity of the immunosensor is proportional to the AFP concentration, showing excellent sensitivity, specificity, and reliability for AFP determination. Comparative studies show the co-coupling is superior than its single coupling counterparts. We envision this unique immunoassay can provide a new avenue for building immunosensors in the fields of disease diagnosis, food safety detection, and environmental monitoring.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF MEMBRANE SCIENCE
·
2021
·
IF 8.742
Qi Chen,Yang He,Yiping Zhao,Li Chen
Functionalized hemodialysis membranes have been employed to suppress oxidative stress for hemodialysis therapy. However, the inhibition effects of the hemodialysis on the harmful metabolites of oxidative stress, including lipid peroxides and advanced glycation end products (AGEs), have not been fully explored yet. Herein, we report on utilizing the tannic acid (TA) coating as a reactive oxygen species (ROS) scavenger onto the hemodialysis membrane surface to solve this problem. Combining high biocompatibility of looped poly(2-ethyl-2-oxazoline) (PEtOx) brushes and anticoagulant property of heparin (Hep), the TA/PEtOx/Hep functionalized poly (ether sulfone) (PES) hemodialysis membrane has constructed by the nucleobase-recognized strategy. The functionalized membrane directly increased the serum total antioxidant capacity, and suppressed lipid peroxidation and protein glycosylation. The TA/PEtOx/Hep functionalized hemodialysis membrane effectively protected cardiomyocytes (H9C2) and vascular endothelial cells (HUVEC) from oxidative damage . The introduction of looped PEtOx brushes indicated in considerable improvement of blood compatibility and reduction of complement activation, and the immobilization of Hep prolonged the activated partial thromboplastin time. The typical toxins were removed in the simulation dialysis experiment, confirming the TA/PEtOx/Hep functionality feasibility. The present work provides a promising candidate for developing and applying the hemodialysis membrane, which might alleviate patients' complications to improve prognostic efficacy.
✓ 已验证
产品关联
DOI
高影响因子
LWT-FOOD SCIENCE AND TECHNOLOGY
·
2020
·
IF 4.006
Guihong Yao,Xiujuan Wang,Minli Yang,Fengming Chen,Yun Ling,Tong Liu,Shige Xing,Meiyi Yao,Feng Zhang
A novel method for one-pot synthesis of functional oil rich in diacylglycerols (DAGs), phytosterol esters (PEs) and α-linolenic acid (ALA) was developed. Firstly, candida rugosa lipase ( CRL ) and thermomyces lanuginosus lipase ( TLL ) were co-immobilized on polydopamine coated Fe 3 O 4 nanoparticles (PDA@Fe 3 O 4 NPs) via covalent bonds. The prepared co-immobilized bi-lipases ( CRL/TLL -PDA@Fe 3 O 4 ) displayed excellent thermal stability, catalytic activity and reusability in comparison to the free forms. Then a rapid one-pot enzymatic synthesis of functional oil was conducted by using CRL/TLL -PDA@Fe 3 O 4 as the biocatalyst. Three kinds of functional oils were produced with high DAGs, PEs and ALA content (≥30.09%, ≥15.61% and ≥26.45%, respectively) under the optimized conditions (1:3:2 phytosterols/ALA/triglycerides, 20 mg/mL CRL/TLL -PDA@Fe 3 O 4 , 40 °C, 8 h). In comparison to a two-step (sequential/immobilized monolipase) synthesis, a significantly synergistic effects occurred in the co-immobilized bi-lipases system. The results demonstrated the feasibility of the novel strategy and would be helpful for the oils and fats industry.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY
·
2019
·Vol. 95
·期 4
·1116-1125
·
IF 2.659
Han Zhang,Shao-Feng Hua,Lei Zhang
BACKGROUND The objective of this research was to use a step-by-step method to prepare a novel biocatalytic system (GO-CEL-E/N-GA-GOD): -OH and -COOH on the surface of graphene oxide (GO) were used to immobilize cellulase (CEL) and glucose oxidase (GOD), respectively, which may be easier to control the loading of enzymes with different kinds, to achieve the one-pot conversion of gluconic acid from carboxymethyl cellulose (CMC). RESULTS The loadings capability of CEL and GOD on GO-CEL-E/N-GA-GOD were 49.07 ± 7.47 mg g −1 and 10.22 ± 2.03 mg g −1 , respectively. The multi-enzyme systems had shallow temperature and pH optima of 40 °C and 5.0. The kinetic constants of GO-CEL-E/N-GA-GOD (of CEL-GOD) were K m = 0.15 ± 0.02 (0.43 ± 0.09) mmol L −1 , V max = 0.18 ± 0.01 (0.21 ± 0.07) μmol L −1 s −1 , and k cat / K m = 24.12 ± 0.52 (17.74 ± 0.85) s −1 mmol −1 L. Nearly 65% of the initial activity of GO-CEL-E/N-GA-GOD could be retained after seven cycles. Notably the conversion of gluconic acid was able to reach 63.82 ± 8.03% within 2 h. CONCLUSION Step-by-step immobilization method made full use of different functional groups on GO, avoiding the competition for fixed sites between CEL and GOD in the immobilization processes. The results reflected the feasibility of the strategy to build bio-microsystem as CEL and GOD immobilized on GO by covalent bonds to achieve the conversion from CMC to gluconic acid in one-step. © 2019 Society of Chemical Industry
✓ 已验证
产品关联
DOI
高影响因子
PROCESS BIOCHEMISTRY
·
2023
·
IF 4.4
Chang Liu,Shuo Liu,Zengyuan Cao,Dajun He,Dongyu Gu,Jing Tian,Yi Yang
α-Amylase inhibitors constitute a category of compounds capable of diminishing α-amylase activity and hold significant promise for the development of novel drugs. This study focused on assessing the potential of Schisandra chinensis Baill extract to inhibit α-amylase. However, sieving and segregating inhibitors from intricate mixtures presents an obstacle. Consequently, this study devised and refined a methodology centered on immobilized α-amylase to identify and isolate natural inhibitors from S. chinensis . The immobilized α-amylase, featuring a load capacity of 254.18 ± 1.20 μg/mg and a specific activity of 2.69 ± 0.01 U/mg, was achieved through meticulous optimization of preparation conditions. Subsequently, this immobilized enzyme was used to isolate bioactive compounds from S. chinensis extract. The results revealed the presence of an active compound with an IC 50 of 324.12 ± 11.23 μg/mL. This compound was isolated via magnetic immobilized enzyme-based ligand fishing, employing high-speed countercurrent chromatography , and was conclusively identified as 5-hydroxymethylfurfural. The molecular docking analyses revealed its ability to interact with the active pocket of α-amylase through multiple hydrogen bonds .
✓ 已验证
产品关联
DOI
高影响因子
Separation Science Plus
·
2023
·Vol. 6
·期 7
·2300026
·
IF 1.1
Qi-Long Hao,Shuo-Wen Wang,Li-Qing Yu,Xiao-Qin Yang,Yun-Kai Lv
The improvement of the strong force between the coating and the substrate is important to make the coating for solid-phase microextraction with good stability, durability, and reproducibility during thermal and solvent desorption. In this paper, zeolitic imidazolate frameworks-90 coating was immobilized on the stainless-steel fiber via the chemical-bonded method with the help of polydopamine. The fiber was used for the solid-phase microextraction of lung cancer biomarkers prior to gas chromatography-mass spectrometry analysis. Several extraction and desorption conditions were optimized. Under the optimized conditions, the established method exhibited high enrichment factors (434–7560), low limits of detection (2.6–4.2 ng/L), wide linear ranges (0.02–30 μg/L), good repeatability (3.46%–9.37% for intra-day, 4.11%–9.18% for inter-day, n = 6) and reproducibility (5.37%–7.02%, n = 3). Moreover, the coated fiber is thermally stable and can be reused at least 100 cycles of extraction/desorption/conditioning without significant loss of extraction efficiency and precision. Eventually, the self-made coated fiber combined with gas chromatography-mass spectrometry was successfully applied to the extraction and detection of five biomarkers of lung cancer from real breath samples with a satisfactory recovery (96.6%–117.6%).
✓ 已验证
产品关联
DOI
高影响因子
Biomed Research International
·
2018
·
IF 2.583
Juan Huang,Yun Yang,Yaokun Wang,Mingyang Zhang,Youxun Liu
The application of laccase-mediator-based catalysis is limited owing to the high cost of laccases and mediators and the potential toxicity of free mediators. Here, a novel biocatalyst (Im-LMS) was fabricated by immobilizing both laccase and a mediator (2,2’-azino-bis-[3-ethylbenzothiazoline]-6-sulfonic acid) on layered double hydroxide/alginate biohybrid beads. The catalytic activity of Im-LMS was evaluated for dye decolorization using malachite green. The decolorization yields of malachite green by Im-LMS and the free laccase-mediator system were 92% within 120 min and 90% within 90 min. Malachite green solution was detoxified completely after biodegradation by Im-LMS. Following eight reuse cycles of Im-LMS for dye treatment, a decolorization yield of 79% was obtained. The activity of Im-LMS was almost completely stable after being stored for 10 days. The recyclability and stability of Im-LMS will be helpful for reducing the running cost and potential toxicity associated with mediators to facilitate practical applications.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Journal of Materials Chemistry B
·
2016
·Vol. 4
·期 8
·1510-1519
·
IF 4.872
Chunbao Du,Xiaoling Hu,Ping Guan,Xumian Gao,Renyuan Song,Ji Li,Liwei Qian,Nan Zhang,Longxia Guo
Surface-imprinted microspheres were controllably synthesized using ionic liquid-functionalized microspheres with a highly cross-linked raspberry-like structure as the matrix via surface molecular self-assembly and precipitation polymerization in aqueous media at room temperature. An immunostimulating hexapeptide from human (IHH) with medical properties was chosen as a template molecule in the preparation of different molecularly imprinted microspheres (MIMs). The experiment process was tracked and the as-prepared microspheres were well characterized. Results reveal that the adsorption capacity and selective recognition of MIMs have a direct relationship with the properties of the functional chain of the ionic liquid-functionalized microspheres. Moreover, MIMs that have both high adsorption capacity and good selective recognition were used in competitive rebinding tests and analysis of urine samples, which demonstrated their potential use for IHH enrichment and in real samples.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
BIOSENSORS & BIOELECTRONICS
·
2015
·
IF 6.409
Biyan Xu,Delun Zheng,Weiwei Qiu,Feng Gao,Shaoxiong Jiang,Qingxiang Wang
In this work, a novel electrochemical DNA biosensor has been developed based on the hybrid film of fern leaf-like α-Fe 2 O 3 microparticles and chitosan (CS). The fern leaf-like α-Fe 2 O 3 microparticles were synthesized via a facile template-free hydrothermal method, and their morphologies were characterized by X-ray diffraction, energy dispersive spectrometry, scanning electron microscope, and transmission electron microscope. Electrochemical characterization assays revealed that the hybrid film modified electrode had remarkable synergistic effects of the large accessible surface area and high electrical conductivity of semiconductive Fe 2 O 3 , and the good film stability of CS. Based on the rich amino groups on CS, the CS–Fe 2 O 3 hybrid film was employed as a functional matrix for probe DNA immobilization using terephthalaldehyde (TPA) as a bifunctional arm-linker. The hybridization capacity of the developed biosensor was evaluated with electrochemical impedance spectroscopy (EIS) using [Fe(CN) 6 ] 3−/4− as the indicating probe. A wide dynamic detection range from 1.0×10 −14 to 1.0×10 −10 M with ultralow detection limit of 5.6×10 −15 M was achieved for the target DNA. The hybridization selectivity experiments further revealed that the biosensor could discriminate fully complementary sequences from one-base mismatched, three-base mismatched, and non-complementary sequences. Moreover, the biosensor showed the advantage of good regeneration ability and reproducibility.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2015
·
IF 2.729
Wailan Xu,Junlin He,Liuliu Gao,Jing Zhang,Junmin Hui,Yanlei Guo,Wenjuan Li,Chao Yu
A simple process in which a graphene oxide (GO)–Prussian Blue (PB)-3,4,9,10-perylenetetracarboxylic dianhydride derivative (PTC-NH 2 ) nanocomposite film is spread onto a glassy carbon electrode (GCE) surface is described. Glucose oxidase (GOD) was adsorbed on the modified glass carbon electrode via the specific structure of hollow Pt nanospheres. This process was sufficient to prevent GOD from leaching away, and to improve the sensitivity of our sensor. To characterise the sensors, several techniques were employed, including transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, cyclic voltammetry (CV), electric impedance spectroscopy (EIS) and chronoamperometry. The resulting amperometric glucose biosensor exhibited a fast response time (within 5 s) and a good linear calibration range from 0.01 ± 0.06 mM to 5.23 ± 0.04 mM with a lower limit of detection of 3.3 μM ( S / N = 3). Possible interferents, including ascorbic acid, l-cysteine, dopamine and ethanol, showed almost negligible electrochemical responses, indicating the high specificity of the proposed biosensor for glucose detection. The improved performance of the proposed electrode for detecting glucose in human serum was ascribed to the high surface-to-volume ratio and the excellent conductivity of the hollow Pt nanospheres. This skilful strategy of incorporating the GO/PB/PTC-NH 2 nanocomposite film with hollow Pt nanospheres enhances the performance of the electrochemical sensor, which holds promising for application in bioassay analysis.
✓ 已验证
产品关联
DOI
高影响因子
BIOTECHNOLOGY AND BIOENGINEERING
·
2024
·
IF 3.5
Han-Yu Liu,Pan Ning,Feng Qian,Yao-Wu Wang,Hai-Min Zhang,Pu Wang
Flavin-dependent halogenase (FDH) is highly prized in pharmaceutical and chemical industries for its exceptional capacity to produce halogenated aromatic compounds with precise regioselectivity. This study has devised a multi-enzyme self-assembly strategy to construct an effective and reliable in vitro coenzyme cycling system tailored for FDHs. Initially, tri-enzyme self-assembling nanoclusters (TESNCs) were developed, comprising glucose dehydrogenase (GDH), flavin reductase (FR) and FDH. The TESNCs exhibited enhanced thermal stability and conversion efficiency compared to free triple enzyme mixtures during the conversion of L -Trp to 6-Cl- L -Trp, resulting in a 2.1-fold increase in yield. Subsequently, an ordered co-immobilization of GDH, FR, and FDH was established, further amplifying the stability and catalytic efficiency of the FDH coenzyme cycle system. Compared to the free TESNCs, the immobilized TESNCs demonstrated a 4.2-fold increase in catalytic efficiency in a 5 mL reaction system. This research provides an effective strategy for developing a robust and efficient coenzyme recycling system for FDHs.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）