Small
·
2025
·
IF 12.1
Jinglang Gong,Hongxiang Huang,Maoyu Gao,Yiling Ruan,Ruifang Chen,Yaoting Li,Shouju Wang,Xiaolian Sun
Nanometal-based therapies face challenges arising from the overexpression of proton efflux transporters in cancer cells, which acidifies the extracellular tumor microenvironment (TME) while preserving a relatively neutral intracellular pH, thereby compromising therapeutic efficacy and fostering an immunosuppressive TME. Here, we integrate the proton pump inhibitor pantoprazole (PTZ) with manganese ferrite nanoparticles (MFNs) within an acidity-responsive polymer for enhanced ferroptosis and cGAS-STING activation mediated immunotherapy. This assembly (PTZ/MFNAs) facilitates tumor accumulation through the enhanced permeability and retention effect while initially restricting the release of metal ions. Upon reaching the tumor site, PTZ release increases intracellular acidity, which further triggers assembly disintegration, accelerates the release of iron and manganese ions, and neutralizes the extracellular microenvironment to alleviate immunosuppression. The released manganese ions synergistically collaborate with iron ions to amplify reactive oxygen species (ROS) generation for ferroptosis while activating the cyclic guanosine monophosphate-adenosine monophosphate synthase-stimulator of interferon genes (cGAS-STING) pathway, stimulating innate immunity. This potentiation of innate immunity, coupled with the reversal of TME immunosuppression, collectively and effectively inhibits tumor growth and metastasis. Therefore, the PTZ/MFNAs co-delivery system represents a promising pH-modulation strategy to enhance iron/manganese ions-mediated ferroptosis and cGAS-STING activation-induced immunotherapy.
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高影响因子
BIOMATERIALS
·
2025
·
IF 12.9
Liqiang Gu,Xintao Sun,Jian Pan,Dongming Liu,Lilan Huang,Yaru Yu,Bing Yu,Hailin Cong
The antibacterial hydrogels have attracted a significant amount of interest for preventing bacterial infection in wound healing. However, irreversible antibiotic resistance and lagging detection of wound infection are still current research pain points for hydrogel wound dressings. This study successfully prepared and characterized a novel aggregation-induced PVA/SA/BBR hydrogel. BBR confers significant antibacterial potential to PVA/SA/BBR hydrogels avoiding the use of antibiotics. The results indicate that PVA/SA/BBR hydrogels displayed antibacterial activity against S. aureus , enhanced wound healing process, negligible cytotoxicity, and low hemolytic activity (< 5%). Molecular docking analysis indicate that BBR could inhibit the activity of FabH of E. coli and S. aureus , with a respective binding energy of -9.0185 and -8.4037 kcal/mol. Moreover, the real-time in situ monitoring and quantifying of wound pH levels could be realized by RGB signals collected and transformed from the visible-fluorescent images. PVA/SA/BBR hydrogels also provided significant fluorescent signals visible to the naked eye due to the AIE effect of BBR when the hydrogel severely loses water. All of these signals suggested anti-infective therapy and hydrogel replacement. Therefore, PVA/SA/BBR hydrogels as efficient non-antibiotic-dependent wound dressings with significant therapeutic effect on skin wounds provide potential applications in wound healing, flexible wearable sensing and drug delivery.
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高影响因子
Journal of Physical Chemistry C
·
2011
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IF 4.524
Li Yuan,Qianqian Tang,Dong Yang,Jin Zhong Zhang,Fayong Zhang,Jianhua Hu
Poly(acrylic acid) grafted mesoporous silica nanoparticles (PAA-MSNs) were prepared by a facile graft-onto strategy, i.e., the amidation between PAA homopolymer and amino group functionalized MSNs. The resultant PAA-MSNs were uniform spherical nanoparticles with a mean diameter of approximately 150 nm, and the graft amount of PAA evaluated by thermogravimetric analysis (TGA) was about 12 wt %. Due to the covalent graft of hydrophilic and pH-responsive PAA, the PAA-MSNs could be well dispersed in aqueous solution, which is favorable to be utilized as drug carriers to construct a pH-responsive controlled drug delivery system. N2 adsorption–desorption isotherm results demonstrated that doxorubicin hydrochloride (DOX), a well-known anticancer drug, could be effectively loaded into the channels of PAA-MSNs through the electrostatic interaction. The loading content and the entrapment efficiency of DOX could reach up to 48% and 95%, respectively. The drug release rate of DOX@PAA-MSN was pH dependent and increased with the decrease of pH. The in vitro cytotoxicity test indicated that PAA-MSNs were highly biocompatible and suitable to use as drug carriers. The drug-loaded DOX@PAA-MSNs were distinctly cytotoxic to HeLa cells, due to the sustained release of drug, and showed higher clinical effects than free DOX. These results imply that the PAA-MSNs are promising platforms to construct pH-responsive controlled drug delivery systems for cancer therapy.
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高影响因子
Materials Today Bio
·
2026
·
IF 11
Xiao-Jun Yu,Bing-Yao Quan,Peng Zou,Si-Bo Wang,Ling-Jiang Li,Rongjin Luo,Yong-Yuan Zhang,Yuan-Ting Zhao
This study developed a pH/ROS dual-responsive SmartGel platform for local CRISPR-Cas9 RNP delivery and TRPV4 inhibition in intervertebral disc degeneration (IVDD). The GelMA/PBA-based SmartGel responded to acidic and oxidative microenvironmental cues and released Cas9/sgRNA under disease-mimicking conditions. In nucleus pulposus (NP) cells, SmartGel-Cas9/TRPV4 lowered ROS accumulation, restored mitochondrial membrane potential (ΔΨm), and coordinated apoptosis, senescence, Ca 2+ homeostasis, CaMKKβ-dependent AMPK-mTOR signaling, and autophagic flux. CaMKKβ knockdown weakened AMPK-mTOR activation and autophagic-flux restoration, supporting CaMKKβ as a required link in this mechanism. In the rat IVDD model, the system preserved disc architecture, improved hydration status, reinforced extracellular matrix (ECM) homeostasis, and restrained downstream pathological signaling. Exploratory scRNA-seq and proteomic analyses further indicated treatment-associated shifts in Ca 2+ /autophagy/MAPK/Wnt-related programs. Overall, SmartGel-Cas9/TRPV4 may attenuate IVDD by restoring CaMKKβ-associated autophagic degradation and ECM balance while limiting aberrant signaling activation. Mechanistic illustration of SmartGel-Cas9/TRPV4 in restoring ECM metabolic balance and treating IVDD. Download: Download high-res image (251KB) Download: Download full-size image
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高影响因子
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2019
·
IF 2.931
Miao Yu,Chun-Shan Zuo,Ning Zhang
Rational design chelating fluorescent sensors probing metal ions in biological system are continuously hot essays nowadays, especially for zinc detection. Herein, a naphthylideneimine based zinc fluorescence probe ( 3 ) was prepared and characterized in this work. Structural features and optical properties of 3 and its metallic complexes were characterized. Fluorescent experiment indicates 3 is extremely sensitive and selective for Zn 2+ with a strong fluorescence enhancement (∼34 folds) in aqueous buffer solution with a limit of detection (LOD) of 3.78 × 10 −7 mol L −1 . Formation constant (log K a ) of the chelating complex of 3 and Zn 2+ ion was determined to be 4.45. Theoretical studies were carried out to get deep insight into the response mechanism in the sensing process. Density functional theory (DFT) methods calculated formation Gibbs free energy (Δ r G m ө ) of the deprotonated complexes model ( 3 2− ⊃ Zn ) is −2.9 kcal/mol, which is in good agreement with the experimental result. The calculation results show that the low excitation states can be ascribe to S0 → T2 and S0 → S1 at 390–430 nm and 310–330 nm, respectively, due to the π → π∗ transition. Finally, yeast cell imaging experiments indicate that 3 can monitor intracellular Zn 2+ as well. These findings would enable this fluorescent probe to be used as a Zinc sensor.
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高影响因子
Materials Today Communications
·
2021
·
IF 3.383
Tingyu Yang,Yangyang Ao,Juanjuan Feng,Cuiyan Wang,Jianbin Zhang
Calcium carbonate (CaCO 3 ) is an ideal candidate for use in drug delivery system (DDS) for cancer cure due to good biocompatibility, pH-sensitivity and low-toxicity. However, developing a method that is fast and green, and have large-scale production of CaCO 3 -based DDS remains a challenge. Here, inspired by biomineralization, for the first time, we develop a one-pot, l -lysine (Lys)-mediated biomineralization method using a CO 2 bubbling procedure to green, simply and quickly prepare CaCO 3 -based DDS. The presence of Lys not only improved the yield of CaCO 3 , but also controled morphology and crystal phase of CaCO 3 . Meanwhile, two different crystal forms of CaCO 3 microspheres were used in the pH-responsive release of cancer drug Sanguinarine (SAN): calcite CaCO 3 (CC) and vaterite CaCO 3 (VC). The structure of the as-prepared CC and VC was characterized by scanning electron microscope (SEM), transmission electron microscope (TEM), Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and Brunauer-Emmett-Teller analysis (BET). The possible formation mechanism was proposed. As a potential DDS, both different DDS showed good drug-loading capacity (CC: 63.5 %; VC: 51.9 %), good biocompatibility, pH-sensitivity and low-toxicity. Meanwhile, after loading the SAN, it had obvious inhibitory effects to cancer cells. However, they presented significant differences in drug loading rate, loading capacity and pH-sensitivity due to differences in crystal form and morphology. This biomineralization method of green synthesis of CaCO 3 -based DDS using CO 2 provided a possibility for industrial application of DDS, and the comparative study of CaCO 3 with different morphologies and crystal forms gave a good idea for the design of DDS.
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高影响因子
JOURNAL OF POLYMER RESEARCH
·
2017
·Vol. 24
·期 8
·122
·
IF 1.615
Zhang Jie,Gong Chu,Li Bingqiang,Shan Meng,Wu Guolin
A magnetic polypeptide nanocomposite with pH and near-infrared (NIR) dual responsiveness was developed as a drug carrier for cancer therapy, which was prepared through the self-assembly of Fe 3 O 4 superparamagnetic nanoparticles, poly(aspartic acid) derivative (mPEG- g -PDAEAIM) and doxorubicin (DOX) in water. Fe 3 O 4 nanoparticles were prepared to provide the superparamagnetic core of nanocomposites for tumor targeting via chemical co-precipitation. The protonable imidazole groups of mPEG- g -PDAEAIM with a pKa of ~7 were accountable for the pH-responsiveness of nanocomposites. The photothermal effect of nanocomposites under the irradiation of NIR laser was induced via the interactions between dopamine groups of mPEG- g -PDAEAIM and Fe 3 O 4 superparamagnetic nanoparticles to trigger the drug release. NMR, FT-IR, TEM, hysteresis loop analysis and MRI were utilized to characterize the materials. The DOX loaded nanocomposites exhibited pH-responsive and NIR dependent on/off switchable release profiles. The nanocomposites without drug loading (Fe 3 O 4 @mPEG- g -PDAEAIM) showed excellent biocompatibility while DOX loaded nanocomposites caused MCF-7 cells’ apoptosis due to the photothermal/chemotherapy combination effects. Overall, the pH and near-infrared dual responsive magnetic nanocomposite had a great potential for cancer therapy.
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高影响因子
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
·
2025
·
IF 8.5
Dong-Wei Shao,Tong Xie,Ya-Tao Wang
Osteoarthritis (OA) is a widespread chronic degenerative joint disease, for which new treatment strategies are urgently needed. In this study, we developed an injectable, pH-responsive hydrogel (HA-TA-CS) aimed at improving joint drug delivery by enhancing retention within the joint cavity, while exhibiting excellent biocompatibility. The hydrogel was loaded with celecoxib for localized, in situ release (HA-TA-CS@Cel). OA is characterized by elevated levels of reactive oxygen species (ROS), which promote oxidative stress, inflammatory cytokine production, and further exacerbate the inflammatory response. HA-TA-CS@Cel effectively mitigates inflammation by scavenging excess ROS through the sustained release of TA and celecoxib. Our results demonstrate that HA-TA-CS@Cel significantly alleviates OA progression. In a mouse OA model, the system reduced pro-inflammatory factors and alleviated disease severity. Furthermore, HA-TA-CS@Cel promoted the polarization of macrophages from the M1 to the M2 phenotype, suggesting its ability to modulate the immune microenvironment and thereby attenuate joint inflammation and slow OA progression. These findings indicate that the injectable HA-TA-CS@Cel hydrogel system holds great promise for treating OA through enhanced cartilage protection and macrophage polarization, with potential applications in other inflammatory diseases.
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iScience
·
2026
·
IF 4.5
Qixian xie,Bowen Guo,Yichen Zhang,Tianze Yang,Chenfang Cui,Zhengliang Liu,Honglin Yao,Baili Li,Wurong Ren
The integration of the KHCO 3 fed bipolar membrane (BPM) electrolyzer with KOH based CO 2 capture enables directly in situ conversion of captured CO 2 into valuable carbonaceous products, eliminating costly CO 2 recovery and compression steps. Even this integrated BPM system assembly holds promise, the critical lack of CO 2 adsorption intermediate species tracking necessitates the development of a monitoring platform. This work pioneers a novel dual-modal strategy employing simultaneous indicator-based sensing with in situ UV-Vis spectroscopy, achieving real time visualization of triphasic dynamics during integrated CO 2 capture-regeneration-conversion. Within this synergistic process involving Ar-saturated KHCO 3 decomposition, and electrochemical reduction, an etched indium (In) catalyst achieves 50% formate Faradaic efficiency (FE) at -1.1 V vs. RHE. The synergistic application of these complementary in situ diagnostics unlocks fundamental mechanistic insights into integrated CO 2 capture and conversion systems, establishing them as critical enablers for advancing efficient and scalable electrochemical CO 2 valorization.
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高影响因子
iScience
·
2026
·Vol. 29
·期 3
·
IF 4.5
Miao Chen,Yan Wang,Yiran Niu,Xiaomin Chen,Hang Su,Liang Xia,Chunbao Liu,Junfen Zhou,Zhen Wang,Bao Li,Diyu Lu
Sonodynamic therapy (SDT) for deep-seated tumors is limited by tumor microenvironment (TME) barriers. We developed a hyaluronic acid (HA)-modified mesoporous calcium carbonate nanoplatform (HA/CaCO 3 @Ce6) to synergistically enhance calcium overload and SDT. The CD44-targeted nanoplatform demonstrated pH-responsive degradation in acidic TME, resulting in the release of Ca 2+ and chlorin e6 (Ce6). The released Ca 2+ induced mitochondrial calcium overload, causing 71% collapse in membrane potential and 1.6-fold increase in reactive oxygen species (ROS) generation, establishing a “Ca 2+ -ROS positive feedback loop.” This synergy triggered robust immunogenic cell death (ICD), enhancing CRT exposure by 94.2%, HMGB1 release by 46.2%, and ATP decrease by 74.5%. In vivo , it achieved 90.9% tumor inhibition and 80% 60-day survival rate, alleviated tumor hypoxia, and inhibited tumor proliferation and angiogenesis. This “ion-interference SDT” paradigm overcomes the limitations of SDT through self-amplified ROS cycle and provides an effective strategy for treating deep-seated solid tumor with significant clinical translation potential.
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LWT-FOOD SCIENCE AND TECHNOLOGY
·
2026
·
IF 7.1
Caiyun Cheng,Qian Xu,Yang Li,Eric Haubruge,Ning Liao,Guangsu Zhu,Kunlun Liu
Whey protein isolate amyloid fibrils (WPIFs) were prepared via acidic thermal treatment at pH 2.0, 85 °C for10 h. The aim was to investigate the influence of different pH values (2.0–8.0) on the structural features of WPIFs and emulsifying properties of WPIFs-stabilized Pickering emulsions. Thioflavin T (ThT) fluorescence confirmed WPIFs formation at pH 2.0. Transmission electron microscopy (TEM) and atomic force microscopy (AFM) revealed that WPIFs maintained long fibrils at pH 2.0–3.0, whereas short fibrils were observed at pH 7.0–8.0. At pH 4.0–6.0, WPIFs underwent aggregation which resulted in increased particle size and decreased zeta potential, with the most distinct aggregates forming at pH 5.0. The structure of WPIFs affected by pH changes determined the emulsification of WPIFs Pickering emulsions. Specifically, WPIFs at pH 5.0 exhibited relatively low emulsifying activity index (EAI) of 14.84 ± 0.37 m 2 /g and high emulsifying stability index (ESI) of 93.25 ± 1.54%. WPIFs Pickering emulsion at pH 5.0 showed increased droplet sizes and enhanced viscoelasticity. The results indicated that the emulsifying properties of WPIFs Pickering emulsions were dependent on the structural features of WPIFs. These findings provide valuable insights into the pH-dependent structural regulation of WPIFs and highlight the application prospects of amyloid fibril-stabilized Pickering emulsions.
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Polymers
·
2026
·Vol. 18
·期 2
·191
·
IF 4.9
Qian-Yu Yuan,Lan Yang,Bing-Chiuan Shiu,Chien-Teng Hsieh,Ching-Wen Lou,Jia-Horng Lin
Infected wounds form a complex microenvironment that creates difficulties for drug delivery. In this study, a composite fiber membrane based on polyethylene oxide (PEO) was prepared. The intention was to achieve on-demand drug release and integrate multiple functions by adjusting the material composition. The membrane uses PEO as the main framework and contains chitosan (CS) and ascorbic acid (Asc). CS leads to an increase in fiber diameter, while Asc makes the fibers thinner. The two components act together to influence the microstructure. In vitro drug release experiments showed that changing the CS content in the PEO matrix can affect the initial release rate and the duration of sustained release. The membrane also shows sensitivity to pH. Under slightly acidic conditions, drug release becomes faster, which is similar to the state of infected wounds. In addition, the membrane maintains antioxidant activity and can inhibit Escherichia coli ( E. coli ) and Staphylococcus aureus ( S. aureus ). These results suggest that PEO-based composite fibers may be useful in drug delivery and tissue repair.
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高影响因子
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