CARBON
·
2020
·
IF 8.821
Ruopeng Zhao,Qinghua Li,Zhijing Chen,Vishal Jose,Xian Jiang,Gengtao Fu,Jong-Min Lee,Shaoming Huang
Rational structure design, composition control and heteroatom doping are efficient strategies to achieve excellent electrocatalysts for the oxygen reduction reaction (ORR) in fuel cells or metal-air batteries . Herein, a facile and efficient approach to prepare ultrathin carbon nanosheet superstructure (BN/C) with high B, N-doping level by using sodium chloride (NaCl)-assisted pyrolysis method is proposed. The developed BN/C catalyst exhibits good catalytic activity for ORR in alkaline medium with a half-wave potential (E 1/2 ) of 0.8 V, which is comparable to that of commercial Pt/C. The BN/C catalyst also shows much better long-term stability and satisfactory tolerance for the methanol crossover effect. This excellent performance is attributed to the structure and composition characteristics of BN/C, including the large surface area (1085 m 2 g −1 ), hierarchically porous structure , the synergistic effect of the B, N co-doping and high content of ORR active species . Significantly, the B element with electron-deficient property in BN/C can create more charged sites favorite for O 2 adsorption and thus accelerate reaction kinetics in ORR. Furthermore, a rechargeable Zn-air battery device comprising BN/C catalyst and RuO 2 with a liquid electrolyte shows superior performance with an open-circuit potential of ∼1.36 V, a peak power density of ∼115 mW cm −2 , as well as excellent durability (1000 cycles for 14 days of operation). Moreover, a flexible solid-state Zn-air battery containing BN/C catalyst and RuO 2 shows good cycling durability under different bending states, indicating the excellent practicability in wearable devices .
✓ 已验证
产品关联
DOI
高影响因子
ARCHIVES OF ORAL BIOLOGY
·
2020
·
IF 1.931
Ying Bian,Juan Xiang
Background Osteogenic differentiation of human periodontal ligament cells (hPDLCs) is crucial for regenerate periodontal tissues. In this study, we investigated the function of salvianolic acid B (Sal B) in osteogenesis of hPDLCs. Methods HPDLCs were isolated from healthy third molar roots. HPDLCs at passage 3 were identified by morphological observation and immunohistochemistry of vimentin . The viability of hPDLCs incubated with Sal B at concentrations of 0μM, 0.1μM, 0.5μM, 1μM and 5μM were measured by CCK-8 assay. To evaluate the effect of Sal B on osteogenic differentiation of hPDLCs, the alkaline phosphatase (ALP) activity, osteogenic differentiation markers, and mineralized nodules were determined by ALP kit, qRT-PCR and alizarin red S staining, respectively. To confirm the function of Sal B in hPDLCs involved in Wnt/β-catenin signaling pathway , hPDLCs were incubated with Sal B or co-incubated with Sal B and DKK-1 (a inhibitor of Wnt/β-catenin). The levels of Wnt/β-catenin signaling pathway and osteogenic differentiation-associated indicators were then determined. Results HPDLCs showed a typical fibroblast-like and spindle-shaped, with vimentin-positive. The viability of hPDLCs had no obvious change with stimulation of Sal B at various doses. Sal B promoted the increase of ALP activity, osteogenic differentiation markers levels, mineralized nodules and activation of Wnt/β-catenin signaling pathway, and DKK-1 could block those effects of Sal B on hPDLCs. Conclusion Sal B promoted osteogenesis of hPDLCs through Wnt/β-catenin signaling pathway, which providing a potential drug for periodontitis treatment .
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Food & Function
·
2020
·Vol. 11
·期 4
·3563-3573
·
IF 4.171
Xing Zhang,Hui He,Tao Hou
Selenium-biofortified crops are a quality functional food resource because of their anti-tumor and anti-cancer properties. In the present study, the conjugates of selenium-biofortified soybean protein and polyphenols were prepared and evaluated by alkali-induced synthesis and in vitro antioxidant tests. Moreover, the antioxidant mechanisms of protecting mice skin damaged by UV-B were studied. The results showed that the antioxidant activity of the conjugate between 7S globulin from selenium-enriched soybean (Se-7S) and EGCG (Se-7S-EGCG) was significantly higher (P < 0.05) than that of Se-7S-GA. Structural characterizations implied that the polymerization of polyphenols with amino acid residues occurred. Se-7S-EGCG inhibited the apoptosis of epidermal cells induced by UV-B. The overexpression of phosphorylated proteins in the MAPK signaling pathway, the activation of related inflammatory factors, and the boost in the MMPs were reversed by Se-7S-EGCG. Overall, this research provides a theoretical and experimental basis for the application of protein and polyphenol conjugates in food and medicine fields.
✓ 已验证
DOI
PubMed
高影响因子
JOURNAL OF CHEMICAL AND ENGINEERING DATA
·
2020
·
IF 2.369
Xiaomei Wang,Zhen Liu,Fa Zhou,Jianhan Huang
Imidazolium salt-incorporated postcross-linked porous polymers, namely, IL-PVB-pc-80%, were developed, and they were evaluated for the adsorption of rhodamine B (RB) and Cd2+ from aqueous solution. After N-vinylimidazole and p-dichloroxylene were inserted in the skeletons, IL-PVB-pc-80% contained both ionized imidazole moieties and plentiful mesopores. The adsorption experiments indicated that IL-PVB-pc-80% has excellent adsorption properties with a maximum capacity of 693.5 mg/g for RB and 373.3 mg/g for Cd2+. The adsorption mechanism revealed that the incorporated imidazolium salts were significantly beneficial for the adsorption.
✓ 已验证
产品关联
DOI
高影响因子
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
·
2020
·
IF 4.192
Xiaofeng Zhang,Xinqi Xu,Xuemei Yao,Rong Wang,Hengtao Tang,Xin Ju,Liangzhi Li
Ribose-5-phosphate isomerase B is of great importance for biocatalysis and biosynthesis, but the multifunctional residues in active sites hinder the research efforts. This study employed rational design strategies to locate the key residues of RpiB from Ochrobactrum sp. CSL1 (OsRpiB). A single-mutant S9T of a noncontact residue showed 80% activity improvement toward d-allose. A double-mutant S98H/S134H further increased the activity to 3.6-fold. The mutations were analyzed by kinetics and molecular dynamics analyses, indicating that S9T might enhance the substrate binding and catalysis by inducing a steric effect, and S98H/S134H could strengthen both ring opening and binding of d-allose. Though S98H/S134H showed low temperature stability, its potential was explored by isomerizing d-allose to d-psicose with higher conversion and in less reaction time. The findings of this study were beneficial for illustrating the complex functions of key residues in RpiBs and applying OsRpiB in preparing rare sugars.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF MATERIALS SCIENCE
·
2020
·Vol. 55
·期 25
·11267-11283
·
IF 3.553
Peng Qian,Dai Ying,Liu Kun,Luo Xianping,He Dongsheng,Tang Xuekun,Huang Ganghong
There is an urgent need for an eco-friendly and efficient route to treat the organic pollutants in wastewater. Therefore, we report a facile synthesis of carbon nanotube–magnesium oxide composite (CNTs/MgO) which can efficiently activate peroxymonosulfate (PMS) to degrade Rhodamine B (RhB). Fabricated CNTs/MgO was characterized by XRD, FTIR, SEM, TEM, BET, XPS, and Raman. The results showed that the carbon nanotubes uniformly entangled on the MgO whiskers and linked to the MgO by Mg–C and C–O bonds. The effects of temperature, pH value, CNTs/MgO dosage, PMS dosage and the ratio of MgO/CNTs on degradation of RhB were conducted to determine the activation performance of CNTs/MgO. The catalytic degradation process was carried out in a wide pH range of 3–9, and 100% of RhB was degraded in 20 min under optimized conditions. Moreover, the efficient degradation performance was attributed to singlet oxygen generated in the PMS&CNTs/MgO system. The excellent activation properties of CNTs/MgO was derived from the enhanced electron transport performance and chemical bonding between MgO and CNTs. In addition, this work provides an innovative design for the development of novel PMS catalysts without transition metal components.
✓ 已验证
产品关联
DOI
高影响因子
Small
·
2020
·Vol. 16
·期 7
·1906634
·
IF 11.459
Zhuosen Wang,Jiadong Shen,Shaomin Ji,Xijun Xu,Shiyong Zuo,Zhengbo Liu,Dechao Zhang,Renzong Hu,Liuzhang Ouyang,Jun Liu,Min Zhu
Lithium–sulfur batteries (LSBs) are considered as one of the best candidates for novel rechargeable batteries due to their high energy densities and abundant required materials. However, the poor conductivity and large volume expansion of sulfur and the “shuttle effect” of lithium polysulfides (LPSs) have significantly hindered the development and successful commercialization of LSBs. Bean-like B,N codoped carbon nanotubes loaded with Co nanoparticles (Co@BNTs), which can act as advanced sulfur hosts for the novel LSB cathode, are fabricated. Uniform graphitic nanotubes improve the conductivity of the electrode and load more electroactive sulfur and buffer volume expansion during the electrochemical reaction. In addition, loaded Co nanoparticles and codoped B,N sites can significantly suppress the “shuttle effect” of LPSs with strong chemical interaction. It is established that the Co nanoparticles and codoped B,N can provide more active sites to catalyze the redox reaction of sulfur cathode. This stable Co@BNTs-S cathode displays an exceptional electrochemical performance (1160 mA h g −1 after 200 cycles at 0.1 C) and outstanding stable cycle performance (1008 mA h g −1 after 400 cycles at 1.0 C with an extremely low attenuation rate of 0.038% per cycle).
✓ 已验证
产品关联
DOI
PubMed
高影响因子
ACS Omega
·
2021
·
IF 3.512
Yuqing Zhao,Hang Yang,Jianfa Sun,Yi Zhang,Shibin Xia
In this paper, phosphoric acid (H3PO4), hydrochloric acid (HCl), and hydrogen peroxide (H2O2) were employed for the modification of oil-based drill cutting ash (OBDCA) for the first time. The adsorption of rhodamine B (RhB) on modified oil-based drill cutting ash (MOBDCA) in an aqueous medium was investigated. H2O2-modified OBDCA had the optimal adsorption efficiency for RhB. The physical and chemical properties of MOBDCA were analyzed using X-ray diffraction (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), ζ-potential, N2 adsorption–desorption isotherm, and pore size distribution. The effect of the pH value (3–11), reaction time (10–720 min), and initial RhB concentration (10–200 mg/L) on RhB adsorption was discussed. The adsorption kinetics highly fitted with the pseudo-second-order model (R2 > 0.99), which indicated that the adsorption process was dominated by chemisorption. The adsorption isotherm fitted well with the Langmuir and Freundlich models (R2 > 0.97), which indicated the monolayer adsorption process and the heterogeneous adsorption process, respectively. The theoretic adsorption capacity (50 mg/g) for RhB was achieved by H2O2-modified OBDCA. This paper provides a promising method of resource utilization of OBDCA to treat organic pollutants.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
CELLULAR SIGNALLING
·
2021
·
IF 4.315
Jinpeng Zhuang,Hua Ning,Maoqing Wang,Wei Zhao,Yongbin Jing,Xiaoqi Liu,Jianing Zu,Pengyu Kong,Xiaoyan Wang,Changhao Sun,Jinglong Yan
During osteoporosis, fat mass and obesity-associated protein (FTO) promotes the shift of bone marrow mesenchymal stem cells to adipocytes and represses osteoblast activity. However, the role and mechanisms of FTO on osteoclast formation and bone resorption remain unknown. In this study, we investigated the effect of FTO on RAW264.7 cells and bone marrow monocytes (BMMs)-derived osteoclasts in vitro and observed the influence of FTO on ovariectomized (OVX) mice model to mimic postmenopausal osteoporosis in vivo. Results found that FTO was up-regulated in BMMs from OVX mice. Double immunofluorescence assay showed co-localization of FTO with tartrate-resistant acid phosphatase (TRAP) in femurs of OVX mice. FTO overexpression enhanced TRAP-positive osteoclasts and F-actin ring formation in RAW264.7 cells upon RANKL stimulation. The expression of osteoclast differentiation-related genes, including nuclear factor of activated T cells c1 (NFATc1) and c-FOS, was upregulated in BMMs and RAW264.7 cells after FTO overexpression. FTO overexpression induced the phosphorylation and nuclear translocation of factor-kappa B (NF-κB) p65 in BMMs and RAW264.7 cells exposed to RANKL. ChIP and dual-luciferase assays revealed that FTO overexpression contributed to RANKL-induced binding of NF-κB to NFATc1 promoter. Rescue experiments suggested that FTO overexpression-mediated osteoclast differentiation was suppressed after intervention with a NF-κB inhibitor pyrrolidine dithiocarbamate . Further in vivo evidence revealed that FTO knockdown increased bone trabecula and bone mineral density, inhibited bone resorption and osteoclastogenesis in osteoporotic mice. Collectively, our research demonstrates that downregulated FTO inhibits bone resorption and osteoclastogenesis through NF-κB inactivation, which provides a novel reference for osteoporosis treatment.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
Nano Energy
·
2019
·
IF 15.548
Shenghua Ye,Feiyan Luo,Tingting Xu,Pingyu Zhang,Hongdong Shi,Shiqi Qin,Jiaping Wu,Chuanxin He,Xiaoping Ouyang,Qianling Zhang,Jianhong Liu,Xueliang Sun
The development of the hydrogen evolution reaction (HER) in alkaline electrolyte is highly desirable but is hindered by sluggish water dissociation and the high cost of Pt–based catalysts. In this study, a facile pyrolyzation method is proposed for synthesizing the Ru nanoclusters (NCs) anchored on B/N–doped graphene (BNG) (denoted as Ru NCs/BNG). During the pyrolyzation, boron was doped into graphene and facilitated the formation of ultra−small Ru NCs with a diameter of 0.5–1 nm. The electronic interaction between B and Ru NCs accelerated the cleavage of H–OH, altering the HER mechanism and lowering the activation energy of H 2 formation, endowing Ru NCs/BNG with excellent alkaline HER activity, even surpassing that of Pt/C. This study reveals the importance of water dissociation barriers for alkaline HER activity and provides a new strategy for improving the HER catalytic activity .
✓ 已验证
产品关联
DOI
高影响因子
BIOTECHNOLOGY LETTERS
·
2019
·Vol. 42
·期 2
·269-276
·
IF 2.154
Cai Xinyong,Zhang Mengjie,Wei Wei,Zhang Yinjun,Wang Zhao,Zheng Jianyong
A novel enzyme immobilization method employing metal–organic framework (MOF) encapsulation and macroporous resin adsorption was developed in this study. Candida antarctica lipase B (CALB) was firstly encapsulated onto metal–organic frame structures (Zeolitic imidazole framework-8, ZIF-8) and further bonded to macroporous resin by physical adsorption. Under optimized immobilization conditions, the activity of the prepared immobilized lipase (CALB-ZIF-8@D101) determined via the methyl esterification of oleic acid was 38.4 U/mg. Compared with free lipase, the immobilized lipase exhibited improved thermal and operational stability and organic solvent tolerance. These results demonstrate that the immobilization method of ZIF-8 encapsulation and macroporous resin adsorption enhanced enzyme properties at a superior level.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
JOURNAL OF ETHNOPHARMACOLOGY
·
2019
·
IF 3.414
Bin Xie,Yuan-Yuan Lu,Zhuo-Hui Luo,Zhao Qu,Chun-Ge Zheng,Xin-An Huang,Hong-Yan Zhou,Ying-Jie Hu,Xiao-Ling Shen
Ethnopharmacological relevance Marsdenia tenacissima (Roxb.) Wight et Arn is a vine distributed in southwest area of China and used in folk medicine for treatment of tumors. Recent decades of studies on this plant reveal its synergistic effects with certain anticancer drugs in cancer therapy. In our previous study, an extract ETA which contains total aglycones made from M. tenacissima significantly enhanced antitumor activity of paclitaxel in tumor-bearing mice. However, the effective constituents in ETA and the underlying mechanisms remain unclear. Aim of the study Reveal the active components in ETA as well as the molecular mechanism in enhancing antitumor efficacy of paclitaxel. Material and methods Main constituents in ETA were purified by chemical methods. Effects of the purified constituents on metabolic activity of CYP450 enzymes were evaluated in human liver microsomes. Ability of the constituents to enhance antitumor activity of paclitaxel were investigated in nude mice bearing HeLa tumors. Pharmacokinetic study was performed in SD rats . Molecular docking was carried out for investigation of drug-protein interactions. Results Three main C 21 steroidal aglycones, 11α- O -tigloyl-12β- O -acetyl-tenacigenin B ( MT1 ), 11α- O -2-methylbutanoyl-12β- O -tigloyl-tenacigenin B ( MT2 ) and 11α- O -2-methylbutanoyl-12β- O -acetyl-tenacigenin B ( MT3 ), together with tenacigenin B ( MT4 ) was prepared from ETA. Among them, MT1 , MT2 and MT3 strongly inhibit the metabolic activity of CYP3A4 . MT2 also showed inhibitory effects on CYP2C8 , CYP2B6 and CYP2C19 . In HeLa tumor xenografts, MT1 , MT2 and MT3 (30 mg/kg) did not affect tumor growth themselves, but significantly enhanced paclitaxel-induced growth inhibition. In addition, coadministration of MT2 with paclitaxel resulted in significant reduction of liver CYP2C8. In pharmacokinetic study, MT2 significantly increased the blood concentration of paclitaxel with increased AUC value by 2.2–5.3 folds. Molecular docking analysis suggested hydrophobic interaction modes of tenacigenin B derivatives with CYP3A4, and also the essential roles of the C-11 and C-12 ester groups for effective interaction with CYP3A4. Conclusion Our study proves that, 11α- O -tigloyl-12β- O -acetyl-tenacigenin B, 11α- O -2-methylbutanoyl-12β- O -tigloyl-tenacigenin B and 11α- O -2-methylbutanoyl-12β- O -acetyl-tenacigenin B, which are the main constituents of ETA, are active inhibitors of CYP3A4 with potential to increase therapeutic efficacy of anticancer drugs that are substrates of CYP3A4. Tenacigenin B derivatives with C-11 and C-12 ester group substitutions, or at least a large part of them, are active components in ETA and M. tenacissima to enhance in vivo antitumor efficacies of paclitaxel.
✓ 已验证
产品关联
DOI
PubMed
高影响因子
危险品化学品经营许可证（带存储）