Small
·
2025
·
IF 12.1
Lixian Huang,Fei Lv,Yidong Bin,Jingjin Zhao,Caiying Li,Shulin Zhao,Shengqiang Hu,Liangliang Zhang
Hydrogen sulfide (H 2 S) is a vital gaseous signaling molecule that plays a central role in various physiological and pathological processes. Given the complementary advantages of fluorescence (FL) and photoacoustic (PA) imaging, there is a growing demand for dual-ratiometric probes that enable precise in vivo monitoring of H 2 S levels. In this study, the use of 2-mercapto-1,3,4-thiadiazole (MTD) as a novel recognition group of H 2 S is presented for the first time, following conjugation with cyanine dyes to obtain a new PA probe Cy-MTD. To achieve dual-ratiometric imaging, Cy-MTD is incorporated into down-conversion nanoparticle (DCNP), resulting in the creation of a pioneering NIR-II FL/NIR-I PA dual-ratiometric nanoprobe DCNP@Cy-MTD. Cy-MTD undergoes the blueshift in absorption from 840 to 670 nm after reaction with H 2 S, enabling NIR-I ratiometric PA imaging of H 2 S by measuring the ratio of PA signal at 670 and 840 nm (PA 670 /PA 840 ). In addition, due to the strong absorption of Cy-MTD ≈840 nm and the overlapping between the absorption spectrum of Cy-MTD and 808 nm excitation band of DCNP, the 808 nm-excited FL emission (F 1550 nm,808Ex ) of DCNP in DCNP@Cy-MTD nanoprobe is quenched through the competitive absorption, while it is restored upon the interaction with H 2 S because of the blueshift in absorption of Cy-MTD. Using the stable FL emission of DCNP under 980 nm excitation (F 1550 nm,980Ex ) as the reference signal, NIR-II ratiometric FL imaging (F 1550 nm,808Ex /F 1550 nm,980Ex ) of H 2 S is achieved. The dual-ratiometric response features of the DCNP@Cy-MTD nanoprobe offer a significant advancement over traditional single-signal or single-modality imaging techniques. By providing enhanced accuracy and reliability, this probe allows for the diagnosis of hepatitis by monitoring the H 2 S, surpassing the capabilities of conventional histopathological methods, which provides a new way for more effective diagnostic strategies for liver diseases.
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高影响因子
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
·
2020
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IF 3.004
Pengru Liu,Xiongdiao Lan,Muhammad Yaseen,Kungang Chai,Liqin Zhou,Jianhua Sun,Ping Lan,Zhangfa Tong,Dankui Liao,Lixia Sun
Purification of small bioactive peptides from complex biological samples is a difficult task due to the interference of concentrated large biomolecules. In this study, a magnetic immobilized metal affinity chromatography matrix modified by poly (ethylene glycol) methyl ether (IMACM@mPEG) was prepared and applied for the rapid purification of angiotensin I-converting enzyme (ACE) inhibitory peptides from casein hydrolysate. The proposed IMACM@mPEG considerably reduced the non-specific adsorption of large proteins and exhibited improved purification efficiency towards ACE inhibitory peptides. A novel peptide with moderate ACE inhibitory activity (IC 50 value of 274 ± 5 μM) was identified as LLYQEPVLGPVR. Lineweaver-Burk plot confirmed the non-competitive inhibition pattern of LLYQEPVLGPVR. The purified peptide was digested after simulated gastrointestinal digestion and produced shorter peptides which contributed to enhanced ACE inhibitory activity. These results indicated that the IMACM@mPEG is an effective method for the prepurification of ACE inhibitory peptide and the purified peptide LLYQEPVLGPVR may have potential as nutraceutical ingredient in functional foods for hypertension treatments.
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高影响因子
Surfaces and Interfaces
·
2024
·
IF 5.7
Xueling Wei,Yuanhao Yang,Zhen Ma,Qiujie Li,Qian Sun,Dekai Zhang,Enzhou Liu,Hui Miao
Bismuth oxysulfide (Bi 2 O 2 S), as a unique new two-dimensional layered material, has gradually attracted the interest of researchers because of its excellent photoelectric properties. How to enhance its charge kinetics is the key to improve photoelectrochemical (PEC) properties. Herein, the cascade type II-I heterostructure of Bi 2 O 2 S/Bi 2 S 3 /ZnIn 2 S 4 O (oxygen doping) was rationally fabricated by oxygen doping for efficient PEC water splitting through one-step hydrothermal method. Bi 2 S 3 was in close contact with Bi 2 O 2 S through Bi atom sharing, while ZnIn 2 S 4 O has enhanced polarity. The double-synergistic effect of the cascade II-I heterojunction with strong interface interaction and asymmetric crystal modification could greatly promote the transfer efficiency of the surface charge and the bulk charge, inhibited the photogenerated carrier recombination, and enhanced the overall charge dynamics. The results showed that the photocurrent density of Bi 2 O 2 S/Bi 2 S 3 /ZnIn 2 S 4 O composite photoanode was 4.82 mA cm −2 under the condition of 1.23 V vs. RHE, which was 9.09 times with that of monomer Bi 2 O 2 S. This study opened up a new way for the application of Bi 2 O 2 S-based photoelectrodes in the field of PEC water splitting.
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Journal of Macromolecular Science Part B-Physics
·
2022
·
IF 1.366
Zengze Chu,Chen Liu,Xinxi Zhang
Copper(I) thiocyanate (CuSCN) is a cost-effective and excellent inorganic p-type semiconductor; it has attracted considerable attention as hole-transporting material for highly efficient perovskite solar cells (PSCs) in recent years. However, various interface issues are currently regarded as the main obstacles for the CuSCN-based PSCs. Herein, we designed and synthesized a star-shaped oligomer (SFX-10) based on a spiro(fluorene-9,9’-xanthene) core terminated with fluorene groups by a simple Sonogashira coupling reaction, and its molecular structure was characterized with 1H, 13C NMR spectra and a high-resolution mass spectrum. The thermal, optical, and electrochemical properties of SFX-10 were fully investigated. SFX-10 shows good thermal stability with a high glass transition temperature of 138 °C. The photovoltaic performance measurements demonstrated that the CuSCN-based PSC using SFX-10 as an interlayer shows an evident improvement in power conversion efficiency of 6.61% compared with 5.52% for the bare device due to the passivation effect of the perovskite surface and the enhancement of the interfacial contact between the perovskite and CuSCN layers.
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HYDROMETALLURGY
·
2026
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IF 6.3
Yangxiang Huang,Mengyao Chen,Lixin Xue,Hangqing Xue,Juexin Wang,Fei Huang,Congjie Gao
To simultaneously increase the Mg 2+ /Li + separating performance under high Mg 2+ /Li + ratios of polyethylene/polyamide (PE/PA) thin film composite (TFC) nanofiltration (NF) membranes, 1,4,7,10-tetraazacyclododecane (Cyclen) was added in the aqueous phase with polyethyleneimine (PEI) monomer. During reverse interface polymerization (RIP) on polyethylene (PE) substrates, phthaloyl chloride (PC) was added to the organic phase with terephthaloyl chloride (TPC) monomer. It was found that the addition of Cyclen and/or PC could both increase the hydrophilicity, surface roughness and average pore sizes of formed virgin PE/PA TFC NF membranes, leading to increased water permeability. The Li + /Mg 2+ separating factor S Li/Mg first increased and then decreased with maximum points at the TPC/PC ratio of 5:1 and Cyclen addition level of 0.02 wt%. Cation absorption, especially Mg(II)-Cyclen interactions in the PA layers under higher Mg 2+ /Li + ratio led to increased surface Zeta potential, decreased average pore diameters μ p, and greatly increased Li + /Mg 2+ separating factor (S Li/Mg ). S Li/Mg value of PEI-TPC/PC 5:1 -Cyclen 0.02 membranes could be increased from 5.0 to 55.47, keeping the water permeability around 6.5 L m −2 h −1 bar −1 when Mg 2+ /Li + ratio increased from 1 to 100 in the brine.
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高影响因子
JOURNAL OF POWER SOURCES
·
2026
·
IF 8.4
Huiyang Xu,Junwei Zhuo,Wenjun Chen,Zhiwei Li,Xiaorui Huang,Yongyi Li,Shuanglong Li,Bo Wang,Junyu Huang,Zimo Wang,Wei Li
A novel Zn 3 In 2 S 6 @Bi 2 Te 3 @C 3 N 5 ternary S-scheme/Type-I heterojunction is constructed. • Topological insulator Bi 2 Te 3 serves as the charge transport bridge. • The catalyst has an exceptional HER rate (42384.11 μmol g −1 h −1 ) with a 29.83% AQY. Addressing the core electrochemical bottlenecks of sluggish interfacial charge transfer and the charge separation-redox capability trade-off in photoelectrochemical water splitting, this work introduces 3D topological insulator Bi 2 Te 3 as a backscattering-free electrochemical charge transport bridge to construct a Zn 3 In 2 S 6 @Bi 2 Te 3 @C 3 N 5 ternary heterojunction with Step-scheme/Type-I synergistic charge transfer. The rationally optimized sample achieves an exceptional visible-light-driven hydrogen evolution rate of 42384.11 μmol g −1 h −1 with an apparent quantum yield of 29.83% at 420 nm, which are 1.68 and 27.43 times higher than those of pristine Zn 3 In 2 S 6 and as-synthesized C 3 N 5 , respectively. The superior performance originates from three synergistic effects: enhanced light harvesting by the multi-component system, promoted carrier separation driven by the robust built-in electric field at heterointerfaces, and improved charge transport efficiency along with optimized hydrogen evolution reaction (HER) kinetics endowed by Bi 2 Te 3 's topological properties. These advantages accelerate electron transport and suppress non-radiative recombination, providing insights for optimizing the kinetics of the HER at the electrochemical interface. Photocatalytic water splitting for hydrogen production offers a promising route to address the energy crisis; however, the rapid recombination of photogenerated carriers in conventional photocatalysts severely limits their efficiency. To overcome this bottleneck, we designed and constructed a novel Zn 3 In 2 S 6 @Bi 2 Te 3 @C 3 N 5 ternary heterojunction photocatalyst. In this system, Bi 2 Te 3 acts as a topological insulator and charge-transport bridge, forming a cascaded band alignment with Zn 3 In 2 S 6 and C 3 N 5 . This establishes a synergistic charge-transfer pathway that effectively promotes the spatial separation of photogenerated electron–hole pairs. The optimized catalyst exhibits outstanding visible-light-driven hydrogen evolution performance, achieving a remarkable HER rate of 42384.11 μmol g −1 h −1 and a high apparent quantum yield of 29.83% at 420 nm, along with excellent long-term cycling stability. This work provides new insights into designing efficient and stable photocatalysts for hydrogen evolution. Download: Download high-res image (381KB) Download: Download full-size image
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ANTIVIRAL RESEARCH
·
2026
·
IF 4.3
Pu Wang,Xinchang Liu,Yong Xie,Yingjun Zhang,Baohua Gu,Jing Li,Yunfu Chen
CAM-A (GLS4) rapidly suppresses HBV DNA with delayed HBsAg decline and ALT flares. • CAM-A upregulates ISGs and activates IFN-α/γ pathways in HBV models. • CAM-A induces TUNEL + caspase-3-independent death of HBcAg + hepatocytes. • CAM-A effects persist without adaptive immunity; synergize with innate immunemodulators such as TLR7 agonist. Capsid assembly modulators (CAMs) are promising antiviral agents for chronic hepatitis B virus (HBV) infection. This study investigates the anti-HBV activity and immunomodulatory effects of Class I CAMs (CAM-A) in vitro and in vivo . In HepAD38 cells, CAM-A treatment significantly upregulated the expression of interferon-stimulated genes (IP-10, MX1, OAS1). In AAV-HBV mice, CAM-A (GLS4) induced rapid reductions in HBV DNA and delayed HBsAg decline accompanied by transient ALT elevation, upregulated interferons and Tnfsf10/Tnfrsf10b gene expression, while terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) and immunohistochemistry (IHC) experiments indicated caspase-3-independent cell death in HBV-infected hepatocytes. Importantly, the antiviral and HBsAg-lowering effects of CAM-A did not require adaptive immunity. Furthermore, combination of CAM-A with a TLR7 agonist in AAV-HBV mice, or with Peg-IFN-α in primary human hepatocytes (PHH) resulted in synergistic antiviral effects, underscoring the therapeutic potential of these agents to promote sustained HBsAg decline and support functional cure strategies in chronic HBV infection.
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ANALYTICA CHIMICA ACTA
·
2026
·
IF 6
Xueyong Qiao,Rongrong Yang,Zhonghui Han,Lei Lü,Xiaolei Zhao,Jinxing He
Background The accurate, ultrasensitive, and on-site detection of pollutants is critical for public health. As a carcinogen, Sudan I is strictly forbidden from use in food products at any levels. However, the conventional method suffers from the high instrument cost and complex procedure, resulting in poor sensitivity, low portability, and long detection time. A ratiometric fluorescence sensor offers a promising prospect for the ultra-rapid, high sensitivity, visual detection, and “point-of-care” testing. The objective of this work was to address the insufficient selectivity of fluorescence probe and enhance the recognition capability and mass transfer rate while diversifying their signal output modes. Results In this study, a novel hollow metal-organic frameworks (MOFs) structural biomimetic nanosensor was developed for the specific recognition and rapid analysis of Sudan I. The sensor separately integrated CsPbCl 1.5 Br 1.5 perovskite quantum dots and CdSe/ZnS quantum dots as dual-emission fluorophores into hollow MOFs-based molecularly imprinted polymers, acting as the recognition element and a signal carrier. The hollow architecture significantly improved mass transfer efficiency, reducing response time to 10 min. The developed nanosensor in instrumental analysis mode exhibited a linearity within the range of 2.00–200.00 μg L −1 at a method's detection limit of 1.00 μg L −1 , indicating high accuracy with recoveries of 88.00–109.65 % in real samples. Furthermore, a smartphone-based portable platform was designed for on-site intelligence detection with a good linear range (0.15–5.00 μg mL −1 ), a method's detection limit of 0.05 μg mL −1 , and recoveries ranging from 80.20 % to 109.00 %, allowing for real-time quantitative monitoring of Sudan I. Significance This study employs hollow MOFs as support materials for in-situ growth of the imprinting layer, ensuring high adsorption capability and rapid binding. The successful implementation of two detection modes provides a comprehensive solution from laboratory precision to field application. Particularly, the portable sensing system enables real-time and on-site quantitative monitoring. This work exhibits the significant potential for the high-performance chemical sensing and point-of-care detection of food contaminants.
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高影响因子
MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 7
·1-10
·
IF 5.7
Shi Tian,Liu Tong,Zhang Jinyuan,Cai Da,Zhang Yueli
An up-conversion molecularly imprinted ratiometric fluorescent probe with a monodisperse nuclear-satellite structure and its test strip are designed which can avoid fluorescent background interference to detect Sudan I in chili powder highly selective and sensitive. The detection mechanism is based on the selective recognition of Sudan I by imprinted cavities on the surface of ratiometric fluorescent probe and the inner filter effect between Sudan I molecules and the emission of up-conversion materials (NaYF 4 :Yb,Tm). Under optimized experimental conditions, the response of fluorescent ratio signals (F 475 /F 645 ) of this test strip show a good linear relationship in the range 0.02–50 μM Sudan I. The limits of detection and quantitation are as low as 6 nM and 20 nM, respectively. Sudan I is selectively detected in the presence of fivefold higher concentrations of interfering substances (imprinting factor up to 4.4). Detection of Sudan I in chili powder samples show ultra-low LOD (44.7 ng/g), satisfactory recoveries (94.99–105.5%) and low relative standard deviation (≤ 2.0%). This research offers a reliable strategy and promising scheme for highly selective and sensitive detection of illegal additives in complex food matrix via an up-conversion molecularly imprinted ratiometric fluorescent test strip. Graphical abstract
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FOOD CHEMISTRY
·
2022
·
IF 9.231
Jinxing He,Lu Yu,Yandu Jiang,Lei Lü,Zhonghui Han,Xiaolei Zhao,Zhixiang Xu
All-inorganic cesium lead halides (CsPbX 3 , X = Cl, Br, I) perovskite quantum dots (PQDs) have attracted extensive research attention due to their unique optical properties. However, their instability and sensitivity to air and moisture hinder further use in fluorescent sensing applications. In this work, the construction and application of PQDs encoded molecularly imprinted polymers (MIPs-CsPbX 3 ) for Sudan I detection were proposed. After being encoded the PQDs, the obtained MIPs-CsPbX 3 microspheres exhibited high stability for the external environment, remarkable bright fluorescence, and specific recognition for Sudan I. The fluorescent intensity of the MIPs-CsPbX 3 microspheres was obviously quenched upon loading Sudan I, and good linear responses in the range of 0.5–150 µg L −1 , limit of detection of 0.3 µg L −1 , and good recoveries of 95.27 % to 105.96 % in spiked samples were obtained. The developed fluorescent probe provided a selective and sensitive quantified method for Sudan I detection in food matrices.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
·
2022
·
IF 4.598
Ping Wang,Huikai Ma,Yanfeng Zhu,Wei Feng,Mengyao Su,Sanqiang Li,Hongju Mao
In this work, a novel ratiometric electrochemical aptasensor was designed on the basis of dual-amplification mechanism by using exonuclease I (Exo I)-assisted target recycling and hybridization chain reaction (HCR). The thiol-modified capture DNA (Cp-DNA) was fixed on the surface of the gold electrode (AuE) modified with gold nanoparticles (AuNPs) through Au-S bonds. The ferrocene (Fc)-labeled DNA (Fc-DNA) hybridized with Cp-DNA to form Fc-DNA/Cp-DNA duplex. The presence of carcinoembryonic antigen (CEA) led to the dissociation of Fc-DNA from Fc-DNA/Cp-DNA duplex and initiated Exo Ⅰ-assisted target recycling to digest Fc-DNA, so numerous Fc left away from the electrode surface and resulting in the “signal off” of Fc. Moreover, Cp-DNA was exposed to bind trigger DNA and initiate HCR with the presence of H1 and H2 probes, resulting in the intercalating of numerous methylene blue (MB) molecules and the “signal-on” of MB. This dual signal strategy significantly amplified the decrease of Fc signal and the increase of MB signal, achieving a linear range of 1 pg mL −1 to 100 ng mL −1 with a detection limit of 0.479 pg mL −1 (S/N = 3). Besides, the developed aptasensor exhibited high specificity, good stability and reproducibility. More importantly, the aptasensor had been applied in detection of CEA in diluted human serum and displayed satisfactory anti-interference property. By changing the corresponding aptamer sequences, the designed strategy can be employed to detect other targets, which holds promising application in clinical diagnosis.
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Analytical Methods
·
2022
·Vol. 14
·期 5
·549-559
·
IF 3.532
Qiaoling Wu,Chun Ji,Lingli Zhang,Qili Shi,Yuangen Wu,Han Tao
The work presented here describes a highly sensitive and simple electrochemical sensor for the detection of Sudan I dye based on a nanocomposite made of MoS2 heterogeneous nanosheets (1T@2H-MoS2) and carboxylated carbon nanotubes (cMWCNTs) (1T@2H-MoS2/cMWCNTs). XPS results indicate that the content of 1T phase MoS2 was estimated to be 72% in 1T@2H-MoS2. Electron microscopy results show that the tubular cMWCNTs are uniformly interwoven in MoS2 nanosheets to form a three-dimensional network structure. Due to the synergistic electrocatalytic ability and high electroactive surface area, the 1T@2H-MoS2/cMWCNTs modified electrode demonstrated excellent analytical performance for Sudan I, including simple operation, good stability and a wide linear range from 5.00 × 10−9 to 2.00 × 10−6 mol L−1 and 2.00 × 10−6 to 1.00 × 10−4 mol L−1 with an ultra-low detection limit of 1.56 × 10−9 mol L−1. The recoveries of Sudan I from spiked real samples (chilli powder and ketchup) were in the range of 95.60% to 106.10% with low RSD (<5%), indicating that the 1T@2H-MoS2/cMWCNTs modified electrode is a promising tool for the analysis of illegal Sudan I in food samples.
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