Frontiers in Chemistry
·
2022
·
IF 5.545
Tian Xiaoran,Yu Qian,Kong Xianming,Zhang Miao
The colloidal polystyrene (PS) was synthesized and decorated with silver nanoparticles (Ag NPs). The plasmonic Ag@PS nanocomposite was prepared by loading Ag NPs on PS microsphere through a seed-mediated in situ growth route. The property of Ag NPs deposited on the PS microsphere could be precisely controlled by adjusting the concentration of the chemicals used in the growth medium. The growth step is only limited by the diffusion of growing species in the growth media to the surface of the Ag seed. The Ag@PS prepared via the in situ growth method exhibited two advantages compared with the self-assembled PS/Ag. First, the high-density of Ag NPs were successfully deposited on the surface of PS as the electroless-deposited Ag seed process, which brings nearly three times SERS enhancement. Second, the rapid preparation process for in situ growth method (half an hour, 10 h for the self-assembled method). The PS/Ag could detect Nile blue A (NBA) down to 10–7 M by SERS. Furthermore, the plasmonic Ag@PS SERS substrate was used for pesticide identification. The on-site monitoring malachite green (MG) from fish was achieved by portable Raman spectrometer, and the limit of detection (LOD) was 0.02 ppm. The Ag@PS substrate has also shown capability for simultaneously sensing multiple pesticides by SERS.
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高影响因子
Foods
·
2026
·Vol. 15
·期 20
·3681
·
IF 6
Shengnan Zhan,Yanke Wang,Yihui Yang,Qi Chen,Xizhi Shi,Hui Wang,Wenhua Yang,Xin Zhang,Tao Huang
Herein, a dual-modal detection strategy forSalmonellausing an engineered bifunctional M13 bacteriophage is presented. ASalmonella-specific binding peptide was fused to the pIII protein, while a streptavidin (SA)-binding nanotag was displayed on the pVIII protein. Taking advantage of the approximately 2700 copies of pVIII per phage, the addition of SA-HRP enabled massive enzymatic loading, which served as the key to generating both SERS and colorimetric signals in the presence of TMB substrate. Under optimized conditions (37 °C, ~1.5 h), the assay achieved limits of detection of 3.4 CFU/mL for the colorimetric mode and 2.7 CFU/mL for the SERS mode. The method exhibited excellent specificity and demonstrated satisfactory accuracy in complex matrices includingPenaeus vannamei, beef, and peanuts, highlighting its strong potential for practical application in rapid food safety detection.
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TALANTA
·
2026
·
IF 6.7
Feier Jiang,Dongmei Liu,Guang Yang,Miaomiao Hu,Cheng Ye,Xu Sun,Hui Sun,Maofeng Zhang
An ordered PMMA@Ti 3 C 2 T x -Ag composite array SERS substrate was fabricated using PMMA microsphere templates and in-situ AgNP immobilization. • Synergistic electromagnetic and chemical enhancement generated abundant hotspots and excellent SERS uniformity (RSD < 5%). • Ultrasensitive detection was achieved for CV and 4-ATP down to 10 −9 M, and for diquat/triazophos and thiram down to 10 −8 and 10 −7 M, respectively. • The platform enabled simultaneous pesticide identification and in-situ residue analysis on blueberry and cherry tomato surfaces below EU maximum residue limits. The rapid identification and quantitative detection of pesticides on a portable device is of vital importance for food safety evaluation and human health protection. However, conventional strategies for realizing the reliable, portable and sensitive detection of target analytes are still challenging. In this work, the ordered polymethyl methacrylate (PMMA) microspheres array were employed as template to fabricate PMMA@Ti 3 C 2 T x . Silver nanoparticles (AgNPs) were then uniformly immobilized onto the composite substrate through an in-situ route, generating abundant electromagnetic hotspots. Benefiting from the synergistic electromagnetic and chemical enhancement, a high-performance PMMA@Ti 3 C 2 T x -Ag composite array SERS substrate was successfully fabricated. The prepared array substrate exhibits excellent SERS performance with good uniformity (relative standard deviation, RSD < 5%) and satisfactory stability. It achieves the detection concentration of 10 −9 M for probe molecules of crystal violet (CV) and 4-aminothiophenol (4-ATP). For pesticide detection using standard solutions, the substrate realizes trace detection of diquat and triazophos down to 10 −8 M, while the detection concentration for thiram is 10 −7 M. Taking advantage of the unique molecular fingerprint signature of SERS, the platform enables simultaneous identification of various pesticide molecules. Further practical tests were carried out to perform in-situ analysis of pesticide residues on the surfaces of blueberries and cherry tomatoes. The detectable concentrations of target pesticides are well below the maximum residue limits stipulated by the European Union, demonstrating the great potential of the SERS platform for rapid screening applications in food safety monitoring. These results indicate the promising potential of the sensing platform for rapid on-site monitoring of pesticide residues in complex food matrices. For the spiked fruit-surface experiments, diquat and triazophos were detected down to 10 −7 M, whereas thiram was detected at 10 −6 M. Download: Download high-res image (363KB) Download: Download full-size image
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JOURNAL OF NANOPARTICLE RESEARCH
·
2017
·Vol. 19
·期 11
·1-13
·
IF 2.02
Zhu Jian,Zhang Qi,Zhang Chun-hong,Weng Guo-jun,Zhao Jing,Li Jian-jun,Zhao Jun-wu
In this work, gold nanobones with the length from 50 to 70 nm were synthesized by a seed-mediated method. The plasmonic optical properties and the roles of gold seed amount in regulating the negative curvatures of the end surfaces of the gold nanobones have also been studied. Compared with the gold nanorods, a new middle surface plasmon resonance (SPR) peak appears in the absorption spectra due to the negative curvatures on the end surfaces of the gold nanobones. What is more, the surface enhanced Raman scattering (SERS) activities of gold nanobones are much stronger than that of gold nanorods. By controlling the amount of the gold seed, the wavelength of the middle SPR peak can be adjusted between 560 and 650 nm, and the corresponding negative curvature of the end surfaces could also be fine tuned. When the amount of gold seed reaches saturation, the end surfaces of nanobones have the strongest negative curvature, which results in the greatest SERS activity. This improved SERS has been attributed to the negative curvature-induced formation of the antenna dimers. The orientation of plasmon coupling between the antenna dimers is perpendicular to the nanobones. Thus, the hot spots at the ends of nanobones could always be created when the excitation beam polarization is either parallel or perpendicular to the nanobones. This negative curvature-dependent SERS enhancement lays the foundation for the extensive application of gold nanobones in SERS.
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BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN
·
2021
·Vol. 94
·期 3
·860-868
·
IF 5.488
He Yuhan,Li Lijun,Li Xiaoxuan,Lin Chubing,Zhang Yan
According to its characteristics, borate can recognize and adsorb target glycoproteins and specific binding properties of antigens and antibodies. This article introduces a preparation method for a magnetic immune SERS sensor, and it is used for the specific identification and detection of trace AFP in human serum. The test used AFP as the target protein and 4-mercaptophenylboronic acid (4-MPBA) modified gold-coated silver nanoparticles (Ag@AuNPs) as the signal factor. After the AFP antibody was grafted on the boric acid group, the SERS detection probe (Ag@Au@4-MPBA@Anti-AFP) was obtained. On the surface of boric acid-functionalized (-P) magnetic silica particles, AFP antibody was modified, and the particles were used as magnetic immunocapture probes (Fe3O4@SiO2-P@Anti-AFP). Finally, the sandwich structure complex of “detection probe-target protein-capture probe” was successfully constructed. The prepared magnetic immune SERS sensor exhibits excellent selectivity and high sensitivity for AFP detection; based on the signal-to-noise ratio S/N = 3, the detection limit can reach 1.0 ng/mL; by performing a spike test on AFP in human serum, the sensor can be used to detect AFP in actual samples. The recovery rate was between 85.8% and 105.7%, and the RSD distribution was ≤3.52%. The sensor was easy to prepare, required no complex processing of samples and can achieve rapid detection, so that it is expected to become a new strategy for clinical detection of AFP.
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JOURNAL OF HAZARDOUS MATERIALS
·
2024
·
IF 12.2
Yu Lu,Xiufang Mo,Geng Zhu,Yan Huang,Yingchao Wang,Zhenzhong Yang,Liqiong Gao,Guofang Shen,Yi Wang,Xiangwei Zhao
Practical gas sensing application requires sensors to quantify target analytes with high sensitivity and reproducibility. However, conventional surface enhanced Raman scattering (SERS) sensor lacks reproducibility and quantification arising from variations of "hot spot" distribution and measurement conditions. Here, a ratio-dependent SERS sensor was developed for quantitative label-free gas sensing. Au@Ag-Au nanoparticles (NPs) were filtered onto anodic aluminum oxide (AAO) forming Au@Ag-Au@AAO SERS substrate. 4-MBA was encapsulated in the gap of Au@Ag-Au and served as the internal standard (IS) to calibrate SERS signal fluctuation for improved quantification ability. Combined with headspace sampling method, SO 2 residue in traditional Chinese medicine (TCM) can be extracted and captured on the immediate vicinity of Au@Ag-Au surface. The intensity ratio I 613 cm −1 /I 1078 cm −1 showed excellent linearity within the range of 0.5 mg/kg-500 mg/kg, demonstrating superior quantification performance for SO 2 detection. Signals for concentration as low as 0.05 mg/kg of SO 2 could be effectively collected, much lower than the strictest limit 10 mg/kg in Chinese Pharmacopoeia. Combined with a handheld Raman spectrometer, handy and quantitative TCM quality evaluation in aspect of SO 2 residue was realized. This ratiometric SERS sensor functioned well in rapid on-site SO 2 quantification, exhibiting excellent sensitivity and simple operability.
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高影响因子
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
·
2024
·
IF 3.8
Qiao Yinuo,Wang Xiaoyan,Fan Zhenlin,Song Yuzhu,Zhang Jinyang,Han Qinqin
Tuberculosis is a highly infectious disease caused by the bacterium Mycobacterium tuberculosis , and the spread of this agent has caused serious health problems worldwide. The rapid and accurate detection of M. tuberculosis is essential for controlling the spread of infection and for preventing the emergence of multidrug-resistant strains. In this study, the powerful trans-cleavage ability of CRISPR-Cas12a for ssDNA was combined with a surface-enhanced Raman spectroscopy (SERS)–based strategy to establish a CRISPR-SERS sensor for the hypersensitive detection of M. tuberculosis DNA. We observed a linear relationship between the concentration of M. tuberculosis DNA and the output signal over the range of 5 to 100 pM. The equation describing the standard curve was y = 24.10 x + 1594, with R 2 = 0.9914. The limit of detection was as low as 4.42 pM for genomic DNA, and a plasmid containing an M. tuberculosis –specific sequence was detected at 5 copy/μL. A detection accuracy of 100% was achieved in the analysis of DNA isolated from the sputum of hospitalized patients with tuberculosis. The entire detection process is simple to deploy and only takes 50 min and results in the sensitive and specific detection of M. tuberculosis DNA. This study provides a new method for the detection of tuberculosis. The tool is stable and can be utilized on-site, and it thus broadens the diagnostic application of CRISPR-Cas12a-based sensor technology.
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高影响因子
Journal of Materials Chemistry A
·
2024
·Vol. 12
·期 28
·17151-17157
·
IF 10.7
Zhenli Sun,Ning Wang,Yiyan Zhang,Xunlong Ji,Zijin Hong,Dan Xie,Wentao Zhang,Wenjing Liu,Jingjing Du
Surface-enhanced Raman scattering (SERS) is a pivotal analytical technique in sensing, yet it grapples with challenges such as reproducibility, substrate stability, and hotspot uniformity. The use of highly effective external field nanocomposites facilitates the dynamic manipulation of “hotspots”—the nanoscale gaps between metal nanoparticles—which is critical for SERS enhancement. The gap between 4-mercaptopyridine-modified (4-MPY) Fe3O4@SiO2@Au nanocomposites (FA) can be precisely reduced by applying an external magnetic field, resulting in up to a 30-fold increase in SERS signal intensity and an 8–39 times increase in the enhancement factor (EF). This approach markedly enhances SERS signal uniformity, demonstrated by the relative standard deviation of the EF dropping from 59.15–9.44% to 28.70–1.14%. By systematically studying the correlations between the SERS EF and the external-magnetic-field strength, hotspot density, and probe molecular density, sensitive magnetic, SERS platforms are established. The optimal hotspots occur in the magnetic field of 80 to 120 mT among the FA-4MPY nanocomposites, as validated by COMSOL finite-element analysis simulations. These findings offer a nuanced understanding of magnetic SERS hotspot tuning, paving the way for precise and improved molecular sensing.
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LAB ON A CHIP
·
2024
·Vol. 24
·期 24
·5384-5397
·
IF 6.1
Siyue Xiong,Chushu Zhu,Chengxuan Wang,Peitao Dong,Xuezhong Wu
Cortisol, known as the “stress hormone”, is secreted by the adrenal cortex. Measuring cortisol levels in body fluids is essential for evaluating stress levels, adrenal function, hormone imbalance, and psychological well-being. Early diagnosis and management of related conditions depend on this measurement. A rapid detection method that combines immunoassay and surface-enhanced Raman scattering (SERS) technology has become widely used in bioanalysis, offering benefits such as fast detection, high throughput, integrated microsystems, and high specificity. This study introduces a pump-free microfluidic chip integrating a solid-state SERS substrate to detect trace amounts of cortisol in bodily fluids through immunoassay. The method relies on a competitive reaction between cortisol and SERS tags with cortisol antigens immobilized on gold nanostructured substrates in a microfluidic environment. Two detection channels are used to provide controls and enhance measurement efficiency and accuracy. Solid-state gold nanostructured substrates offer a larger surface area for antibody capture and act as SERS-active substrates, which significantly enhance the Raman signal and improve the microsystem's sensitivity and applicability. Driven by a capillary pump, the sample can be loaded within 60 seconds, with the entire detection process taking less than 10 min, significantly reducing the detection time. Results indicate that the detection limit for cortisol is 10 pg mL−1, meeting clinical biomarker thresholds. The integrated SERS microfluidic chip shows great promise as an analytical tool for the rapid and sensitive diagnosis of cortisol in bodily fluids.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2024
·
IF 4.3
Wenhui Li,Yingxin Chen,Xin Li,Yi Zhong,Pei Xu,Yuanjie Teng
Synthetic antioxidants serve as essential protectors against oxidation and deterioration of edible oils, however, prudent evaluation is necessary regarding potential health risks associated with excessive intake. The direct adsorption of antioxidants onto conventional surface-enhanced Raman scattering (SERS) substrates is challenging due to the presence of phenolic hydroxyl groups in their molecular structures, resulting in weak Raman scattering signals and rendering direct SERS detection difficult. In this study, a diazo derivatization reaction was employed to enhance SERS signals by converting antioxidant molecules into azo derivatives, enabling the amplification of the weak Raman scattering signals through the strong vibrational modes induced by the N = N double bond. The resulting diazo derivatives were characterized using UV–visible absorption and infrared spectroscopy, confirming the occurrence of diazo derivatization of the antioxidants. The proposed method successfully achieved the rapid detection of three commonly used synthetic antioxidants, namely butylated hydroxyanisole (BHA), tert -butylhydroquinone (TBHQ), and propyl gallate (PG) on interfacial self-assembled gold nanoparticles. Furthermore, rapid predictions of BHA, PG, and TBHQ within the concentration range of 1 × 10 −6 to 2 × 10 −3 mol/L were achieved by integrating a convolutional neural network model. The predictive range of this model surpassed the traditional quantitative method of manually selecting characteristic peaks, with linear coefficients (R 2 ) of 0.9992, 0.9997, and 0.9997, respectively. The recovery of antioxidants in real soybean oil samples ranged from 73.0 % to 126.4 %. Based on diazo derivatization, the proposed SERS method eliminates the need for complex substrates and enables the analysis and determination of synthetic antioxidants in edible oils within 20 min, providing a convenient analytical approach for quality control in the food industry.
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高影响因子
CHEMICAL ENGINEERING JOURNAL
·
2025
·
IF 13.2
Hongwen Cao,Yunpeng Cao,Jia He,Xin Xie,Zhijun Wang,Sen Sun,Rui Zhong,Lei Sun,Yihan Wang,Kun Zhang
Surface-enhanced Raman spectroscopy (SERS) of MoO 3 semiconductors leverages charge-transfer (CT) pathways to achieve highly sensitive detection of toxic molecules, demonstrating significant potential for multiple applications. However, insufficient CT pathways and efficiencies are key factors limiting the development. In this work, W-doped MoO 3 substrates with different doping ratio were prepared using double-target magnetron sputtering method to increase the CT pathway formation. UV–Vis and UPS analyses indicate that the enhancement of SERS activity was mainly due to the narrowing of the optical bandgap and the optimization of band edge positions, which promoted the formation of photo induced charge transfer pathways. The W-doped MoO 3 substrates significantly elevated the SERS response by several orders of magnitude compared to the original value, achieving an enhancement factor (EF) of CV up to 1.31 × 10 6 . Importantly, the W-doped MoO 3 substrates have proven capable of simultaneously detecting two types of pollutant molecules in river water affected by industrial emissions, highlighting their practical application potential in environmental monitoring.
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MICROCHEMICAL JOURNAL
·
2024
·
IF 4.9
Linfan Guo,Ganhong Zheng,Xiujuan Wang,Yupeng Yuan,Chuhong Zhu
Surface-enhanced Raman scattering (SERS) technique can offer the fingerprint information of trace substances, and thus has wide potential applications in many fields. There is still a requirement to develop new and effective methods to construct high-performance SERS substrates. Here, a facile electrodeposition method is presented for fabricating large-scale uniform films assembled by branched silver (Ag) nanoplates . The morphology and structure of such film are highly uniform over a large area, which can ensure good spectral uniformity and reproducibility. There are dense sub-10 nm gaps between neighboring Ag nanoplates and between neighboring branches, forming plenty of hot spots. Therefore, the Ag nanoparticle monolayer exhibits high SERS activity along with excellent spectral repeatability. It is found that the limits of detection (LODs) towards rhodamine 6G and 4-aminothiophenol can reach 0.387 pM and 49.0 pM, respectively, showing high sensitivity of the designed SERS substrate. The enhancement factor of the prepared roughened Ag nanoparticle monolayer is computed to be as large as 2.37 × 10 8 . Then, the highly roughened nanoparticle monolayer is used to detect cationic dye and fungicide (methylene blue, MB) and a synthetic cytokinin (6-benzylaminopurine, 6-BAP), and the corresponding LODs are determined down to 1.1 nM and 28.8 pM, respectively. Such LODs are much lower than the tolerance for MB and 6-BAP residues established by some authoritative regulations. Sensitive monitoring of 6-BAP residue on vegetables and fruits can also be achieved using such SERS substrate, indicating its promising application in environment monitoring and food safety supervision.
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