JOURNAL OF NUTRITIONAL BIOCHEMISTRY
·
2023
·
IF 5.6
Mingzhi Zhu,Jian Ouyang,Fang Zhou,Chenjie Zhao,Wan Zhu,Chunfang Liu,Peifang Huang,Jiafeng Li,Junwei Tang,Zhang Zhang,Jianan Huang,Miaomiao Wu,Kunbo Wang,Zhonghua Liu
Fu brick tea (FBT) is a traditional tea manufactured by solid-state fermentation of tea leaves ( Camellia sinensis ). Although anti-obesity effects have been reported for FBT, the associated role of FBT polysaccharides (PSs) and the underlying mechanisms remain unknown. In this study, we found that FBTPSs inhibited obesity, hyperlipidemia , and inflammation; improved intestinal barrier function; and alleviated gut microbiota dysbiosis in high-fat diet-fed rats. Akkermansia muciniphila , Bacteroides , Parasutterella, Desulfovibrio , and Blautia were the core microbes regulated by FBTPSs. FBTPSs regulated the production of gut microbiota-related metabolites, including short-chain fatty acids (SCFAs), branched-chain amino acids , and aromatic amino acids throughout the development of obesity, and regulated the SCFA-GPR signaling pathway . FBTPS-treated fecal microbiota transplant ameliorated obesity, alleviated gut microbiota dysbiosis, and improved gut microbiota-associated metabolites, suggesting that the anti-obesity effect of FBTPSs was gut microbiota-dependent. FBTPSs may serve as novel prebiotic agents for the treatment of obesity and dysbiosis of gut microbiota.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2021
·Vol. 19
·期 1
·1-20
·
IF 10.435
Xu Shibo,Chang Linna,Hu Yanan,Zhao Xingjun,Huang Shuocheng,Chen Zhenhua,Ren Xiuli,Mei Xifan
Background Healing of MRSA (methicillin-resistant Staphylococcus aureus) infected deep burn wounds (MIDBW) in diabetic patients remains an obstacle but is a cutting-edge research problem in clinical science. Surgical debridement and continuous antibiotic use remain the primary clinical treatment for MIDBW. However, suboptimal pharmacokinetics and high doses of antibiotics often cause serious side effects such as fatal complications of drug-resistant bacterial infections. MRSA, which causes wound infection, is currently a bacterium of concern in diabetic wound healing. In more severe cases, it can even lead to amputation of the patient's limb. The development of bioactive nanomaterials that can promote infected wound healing is significant.Results The present work proposed a strategy of using EGCG (Epigallocatechin gallate) modified black phosphorus quantum dots (BPQDs) as therapeutic nanoplatforms for MIDBW to achieve the synergistic functions of NIR (near-infrared)-response, ROS-generation, sterilization, and promoting wound healing. The electron spin resonance results revealed that EGCG-BPQDs@H had a more vital photocatalytic ability to produce singlet oxygen than BPQDs@H. The inhibition results indicated an effective bactericidal rate of 88.6% against MRSA. Molecular biology analysis demonstrated that EGCG-BPQDs significantly upregulated CD31 nearly fourfold and basic fibroblast growth factor (bFGF) nearly twofold, which were beneficial for promoting the proliferation of vascular endothelial cells and skin epidermal cells. Under NIR irradiation, EGCG-BPQDs hydrogel (EGCG-BPQDs@H) treated MIDBW area could rapidly raise temperature up to 55 °C for sterilization. The MIBDW closure rate of rats after 21 days of treatment was 92.4%, much better than that of 61.1% of the control group. The engineered EGCG-BPQDs@H were found to promote MIDBW healing by triggering the PI3K/AKT and ERK1/2 signaling pathways, which could enhance cell proliferation and differentiation. In addition, intravenous circulation experiment showed good biocompatibility of EGCG-BPQDs@H. No significant damage to major organs was observed in rats.Conclusion sThe obtained results demonstrated that EGCG-BPQDs@H achieved the synergistic functions of photocatalytic property, photothermal effects and promoted wound healing, and are promising multifunctional nanoplatforms for MIDBW healing in diabetics.Graphical Abstract
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Analytical Methods
·
2021
·Vol. 13
·期 9
·1154-1163
·
IF 2.896
Chang Yin,Qianfen Zhuang,Qin Xiao,Yong Wang,Jianhua Xie
A ratiometric electrochemical sensor for caffeic acid (CAE) detection was constructed using a glassy carbon electrode modified with poly(methylene blue) and flower-like nickel-based metal organic frameworks (PMB@Ni–TPA/GCE). The electrochemical behavior of CAE was investigated at the PMB@Ni–TPA/GCE, and was found to follow a two-electron, two-proton electrooxidation process. PMB was used as the internal reference probe, and Ni–TPA can enhance the electrochemical signals of both CAE and PMB. As the CAE concentration increases, the oxidation peak current of CAE is enhanced but that of PMB keeps almost unchanged. The oxidation peak current ratio between CAE and PMB recorded by differential pulse voltammetry changes linearly with CAE concentration over the range of 0.25–15.0 μM, with a detection limit of 0.2 μM. The proposed sensor was successfully employed to evaluate the total polyphenolic content as CAE equivalent in chrysanthemum tea, and the results were comparable with those given by the reference Folin–Ciocalteu spectrophotometry.
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FOOD CHEMISTRY
·
2020
·
IF 6.306
Xinlei Yu,Yuchuan Li,Chang He,Jingtao Zhou,Yuqiong Chen,Zhi Yu,Pu Wang,Dejiang Ni
The influence mechanism of different withering methods (CK, indoor natural spreading; LTD, low-temperature plus dark; LTY, low-temperature plus yellow-light; LTCD, low-temperature plus CO 2 ) on non-volatile compounds in postharvest tea leaves was investigated by UHPLC-Q‐TOF/MS-based non-targeted metabolomic and transcriptomic analyses. Compared with CK, low-temperature withering could slow down polyphenol oxidation by inhibiting polyphenol oxidase activity and keeping the expression of genes for flavanol synthesis. After withering, the proteinaceous amino acid content increased significantly, especially for LTCD and LTY, mainly due to increased peptidase activity and up-regulation of genes involved in the biosynthesis of valine, leucine, aspartic acid, glutamic acid, phenylalanine, and proline. Moreover, LTCD and LTY enhanced the synthesis of γ-aminobutyric acid and metabolism of phenylalanine-methyl salicylate and tryptophan-indole, respectively. Meanwhile, the transformation of theobromine to caffeine was accelerated under low-temperature withering. This research provides a genetic metabolic basis for the application of low-temperature withering to actual green tea processing.
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FOOD CHEMISTRY
·
2020
·
IF 6.306
Xi Chen,Hetong Lin,Taotao Xu,Keqiang Lai,Xue Han,Mengshi Lin
Cellulose nanofibers (CNF) coated with inorganic nanoparticles are novel hybrid nanocomposites that have great potential in various areas including agriculture and food science. The objectives of this study were to synthesize nanocomposites consisted of CNF coated with silver nanoparticles (AgNPs), which can be used as a surface-enhanced Raman spectroscopy (SERS) platform for measuring pesticides in Oolong tea. CNF were coated with AgNPs to form uniform CNF-AgNP nanocomposites that were investigated by transmission electron microscopy. Three-dimensional and porous CNF structures were loaded with AgNPs with an average size of 41 nm. CNF-AgNP substrates were applied in characterization and measurement of flusilazole in Oolong tea samples by SERS. A detection limit of 0.5 mg/kg for flusilazole was obtained based on partial least squares (PLS) regression analysis. These results indicate that CNF-AgNP nanocomposites combined with SERS is an accurate, sensitive, and efficient technique for identification and quantification of pesticide residues in Oolong tea.
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JOURNAL OF APPLIED POLYMER SCIENCE
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2021
·Vol. 138
·期 43
·51261
·
IF 3.125
Nouman Ali Shah,Yue Ren,Ri-Tong Lan,Jia-Cheng Lv,Rizwan M. Gul,Peng-Fei Tan,Shishu Huang,Lin Tan,Jia-Zhuang Xu,Zhong-Ming Li
Highly crosslinked ultrahigh molecular weight polyethylene (UHMWPE) stabilized by vitamin E (VE) is widely applied in artificial joints as the bearings. Despite the approval, there is a discord that VE lowers the crosslinking efficiency, limiting its use at high concentration. In this work, we aim to obtain highly crosslinked and oxidation resistant UHMWPE through the conjunction of tea polyphenol and chemical crosslinking. We hypothesized that highly incorporated tea polyphenol with multiple reactive sites can ameliorate crosslinking efficiency of chemical crosslinked UHMWPE in comparison to VE. Epigallocatechin gallate (EGCG) as representative tea polyphenol was incorporated into UHMWPE at high concentration (2–8 wt%), followed by chemical crosslinking with 2 wt% organic peroxide. Unlike VE/UHMWPE blends as the control, chemical crosslinking achieved an increasing trend in crosslink density of EGCG/UHMWPE blends with increasing antioxidant concentration. High concentration of EGCG also enhanced the oxidation stability of UHMWPE. Intriguingly, EGCG endowed UHMWPE with an excellent antimicrobial property, which was inefficient in VE/UHMWPE. Cell viability was hardly affected by the high loaded antioxidant and peroxide. The chemically crosslinked UHMWPE blended with EGCG is proved to be a reasonable, cost effective and realistic alternative for use in artificial joints.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2021
·
IF 5.279
Yinyin Liao,Haibo Tan,Guotai Jian,Xiaochen Zhou,Luqiong Huo,Yongxia Jia,Lanting Zeng,Ziyin Yang
Tea (Camellia sinensis) is the most popular nonalcoholic beverage worldwide. During cultivation, tea plants are susceptible to herbivores and pathogens, which can seriously affect tea yield and quality. A previous report showed that (Z)-3-hexenol is a potentially efficient defensive substance. However, the molecular mechanism mediating (Z)-3-hexenol signaling in tea plants and the resulting effects on plant defenses remain uncharacterized. To clarify the signaling mechanisms in which (Z)-3-hexenol and light are involved, the gene transcription and metabolite levels were assessed, respectively. This study demonstrated that tea plants rapidly and continuously release (Z)-3-hexen-1-ol in response to an insect infestation. (Z)-3-Hexen-1-ol absorbed by adjacent healthy plants would be converted into three insect defensive compounds: (Z)-3-hexenyl-glucoside, (Z)-3-hexenyl-primeveroside, and (Z)-3-hexenyl-vicianoside identified with laboratory-synthesized standards. Moreover, (Z)-3-hexen-1-ol also activates the synthesis of jasmonic acid to enhance the insect resistance of tea plants. Additionally, a continuous light treatment induces the accumulation of (Z)-3-hexenyl-glycosides. Hence, (Z)-3-hexenol serves as a light-regulated signaling molecule that activates the systemic defenses of adjacent plants. Our study reveals the molecular mechanisms by which biotic and abiotic factors synergistically regulate the signaling functions of herbivore-induced plant volatiles in plants, providing valuable information for future comprehensive analyses of the systemic defense mechanisms in plants.
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JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
·
2019
·Vol. 56
·期 3
·1127-1133
·
IF 1.85
Shu Yang,Li Jie,Yang Xiaoping,Dong Xinyang,Wang Xujie
The bioaccessibilities of polyphenols and polysaccharides in green tea powders (GTPs) with different particle sizes of 564.24 µm, 74.85 µm, 34.62 µm and 15.10 µm and their antioxidant activities were investigated using an in vitro simulated gastrointestinal digestion model. The results showed that particle size significantly affected the bioaccessibilities of polyphenols and polysaccharides before and after digestion, except for the bioaccessibility of polysaccharides after gastric plus intestinal (GI) digestion, thus significantly affecting the antioxidant activity of GTPs. Compared with the undigested initial amount, the bioaccessibilities of polyphenols in all GTPs were approximately 59.98–71.00% after gastric digestion and 9.69–15.57% after GI digestion, and the bioaccessibilities of polysaccharides were approximately 71.10–79.51% after gastric digestion and 113.78–190.38% after GI digestion. With the decrease in particle size, the FRAP value of GTP before digestion was significantly increased and that of 15.10 µm was the largest (4.96 mmol Fe 2+ /g). Both the FRAP and DPPH values after digestion showed a trend of first increasing and subsequently decreasing; 74.85 µm GTP had the largest FRAP (4.11 mmol Fe 2+ /g) and DPPH (156.61 mg VCE/g) values after gastric digestion, and 34.62 µm GTP had the largest FRAP (0.16 mmol Fe 2+ /g) and DPPH (1.43 mg VCE/g) values after GI digestion. This study suggested that the bioaccessibilities of polyphenols and polysaccharides in GTPs and their antioxidant activity can be improved by properly reducing the particle size such that TGPs can exert more beneficial health effects.
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JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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2018
·
IF 2.441
Xiaoling Yang,XiWen Lin,YiDuo Mi,Haixiang Gao,Jing Li,Sanbing Zhang,Wenfeng Zhou,Runhua Lu
In this study, a novel ionic liquid-type surfactant modified attapulgite named as 1-dodecyl-3-methylimidazolium bromide-attapulgite (C 12 MIM-ATP) is successfully prepared and applied in dispersive solid phase extraction (dSPE) for the fast determination of pyrethroid residues in tea drinks. The primary factors that influenced the extraction efficiency, including sorbent type, amount of sorbent, extraction time, desorption conditions, pH and ionic strength , are investigated. The optimized results reveal that the extraction and desorption equilibria are rapidly obtained within 1 min. Under the optimized conditions, good linearity (2–500 μg/L) is observed for four pyrethroids in tea drinks with determination coefficients (r 2 ) ranged from 0.9992 to 1.0000. The limits of detection (LODs) are 0.6 μg/L for all pesticides. Acceptable extraction recoveries of target analytes are found from 90.28 to 107.56% with relative standard deviations (RSDs) less than 8.30% in real tea drink samples. The batch-to-bath repeatability is evaluated by recovery test on five independent synthesized C 12 MIM-ATP sorbents. Satisfactory batch-to-batch repeatability is obtained with the recovery factors varied in 15%. A small matrix effect is observed using C 12 MIM-ATP as the sorbent for detection pyrethroids in tea drinks.
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Analytical Methods
·
2015
·Vol. 7
·期 5
·2093-2099
·
IF 1.821
Wei Xiong,Chunsheng Cheng,Yaling Yang
A method to separate and preconcentrate trace levels of chromium using silver nanoparticle (Ag NP) technology and suspension dispersive solid phase extraction is proposed. The synthesized Ag NPs combine with chromium to form aggregations. Suspension dispersive solid phase extraction is based on the reaction of zinc sulfate (ZnSO4) and sodium hydroxide (NaOH) to form a zinc hydroxide suspension. The UV-vis absorption spectra and transmission electron microscopy (TEM) confirmed the aggregation of the Ag NPs. The influence of several parameters, such as volume of silver nitrate, extraction temperature, the solution pH, type and mass of the sorbent, vortex time, equilibration time, Ag NP volume, and the type, concentration and volume of the acid and foreign ions were investigated and optimized. Linearity was investigated from 10 to 1000 μg L−1, and the correlation coefficient was 0.9988. Recoveries of 97.7–103.3% and an enrichment factor of 20 were achieved with 40 mL of sample. The LOD was 3.2 μg L−1 for total chromium, and the relative standard deviation (RSD) was 4.2% at 20 μg L−1. This method is simple and is successfully applied for the determination of chromium in tea samples with satisfactory results.
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SEPARATION AND PURIFICATION TECHNOLOGY
·
2016
·
IF 3.299
Yin Zhu,Hai-Peng Lv,Wei-Dong Dai,Li Guo,Jun-Feng Tan,Yue Zhang,Fang-Lin Yu,Chen-Yang Shao,Qun-Hua Peng,Zhi Lin
Aroma is one of the most important determinants of tea quality; thus, the precise characterization of the chemical composition of tea aroma is of significant value. In this study, aroma components of the most famous Chinese green tea, Xihu Longjing tea, were first systematically analyzed using comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry (GC × GC-TOFMS) combined with simultaneous distillation extraction (SDE). 478 volatile compounds were identified as common constituents of fifty-five Xihu Longjing tea samples based on standard mass spectra in available databases, retention times, retention indices, structured chromatograms and standards. Subsequently, the aroma qualities of Xihu Longjing tea samples were discriminated by sensory evaluation combined with multivariate analysis. The study results indicated that 37 compounds were positively correlated with aroma quality, and 65 compounds were negatively correlated with aroma quality. The above compounds with various odor characteristics were considered as the key components to affect the aroma quality of Xihu Longjing tea.
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Food Bioscience
·
2024
·
IF 4.8
Ruichun Wang,Songyi Lin,Junbo Chu,Yi Yuan,Simin Zhang
Eleutheronema Tetradactylum is commercially important marine fish and a fixture at tables. However, nonnegligible hazardous substances, such as heterocyclic aromatic amines (HAAs) and advanced glycation end products (AGEs), generates along during thermal processing. Herein, we determined HAAs and two representative AGEs, Nε-carboxyethyl-lysine (CEL) and Nε-carboxymethyl-lysine (CML) formation in roasted Eleutheronema Tetradactylum , and proposed inhibitory method of tea polyphenol to effectively reduce HAAs, CML and CEL, which caused 51.23, 46.72, 24.06 and 35.48 percentage reduction of Norharman, Harman, CML and CEL, respectively, led IQ, 8-MeIQx below detected limitation. Precursors (amino acids, creatin(in)e, glucose and glyoxal), lipid oxidation, fatty acid analysis and further nontargeted lipidomic approaches are attempted to reveal the inhibition mechanism. This work develops a new method to minimize HAAs and AGEs in Eleutheronema Tetradactylum , provide a comprehensive interpretation of inhibition mechanism by tea polyphenols, which is helpful to food manufacturers for healthier fish production.
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