IRANIAN POLYMER JOURNAL
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2023
·
IF 3.1
Ding Ding,Xu Wenzong,Qin Zhongqiong,Cheng Zihao,Zhang Zhihui,Qiao Mengxia
Expandable graphite (EG) is widely used for flame retardancy of rubber. Nevertheless, with the increase in the amount of EG, the mechanical properties of rubber materials drop rapidly and they would not be conducive to the practical application of rubber products. In this study, piperazine pyrophosphate-boron nitride (PPAP-BN) was synthesised by in situ polymerization and combined with EG to prepare SBR composite material. The mixture of PPAP-BN/EG with a mass ratio of 1:4 at 25 phr (parts per hundreds rubber) resulted in a slow thermal degradation process of SBR and achieved the best flame-retardant effect: limiting oxygen index (LOI) increased from 19.5% to 28.5%, peak heat release rate (pHRR) and total heat release rate (THR) decreased by 69.9% and 39.4%, respectively, and the peak smoke release rate (pSPR) and total smoke release rate (TSP) also decreased by 68.0% and 48.1%, respectively. This is mainly due to the synergistic effect of PAPP-BN and EG. The phosphorus-containing groups in PAPP-BN have a promoting effect on carbon formation. The piperazine structure can form a cross-linked carbon layer, whilst the layered structure of boron nitride increases the density of the carbon layer, effectively reducing the heat and smoke release of the material. In addition, the thermal conductivity of SBR composite materials increased by 78.8%. Graphical Abstract
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Nano-Micro Letters
·
2023
·Vol. 16
·期 1
·1-15
·
IF 26.6
Wang Shijie,Zhang Xue,Hao Shuyan,Qiao Jing,Wang Zhou,Wu Lili,Liu Jiurong,Wang Fenglong
An ingenious design achieved magnetic-dielectric-carbon coupling.
Nickel and manganese oxide particles were in situ reduced and grew on the carbon aerogels.
The aerogels demonstrated radar stealth, infrared stealth and thermal management capability.
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JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-23
·
IF 10.2
Guo Rongkang,Wu Chaohan,Liu Fan,Dong Tianhua,Zhang Tao
Objective This study aims to investigate the mechanism by which biomimetic composite hydrogels loaded with bone marrow mesenchymal stem cells (BMSCs) derived microRNA-19b-3p/WWP1 axis through extracellular vesicles (EVs) affect the new bone formation in rat bone defects. Methods First, synthesize the bionic composite hydrogel Gel-OCS/MBGN. Characterize it through field-emission scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), and FTIR. Then, conduct performance tests such as rheology, dynamic mechanical analysis, in vitro mineralization, and degradation. Rat BMSCs were selected for in vitro cell experiments, and EVs derived from BMSCs were obtained by differential centrifugation. The EVs were loaded onto Gel-OCS/MBGN to obtain Gel-OCS/MBGN@EVs hydrogel. Cell viability and proliferation were detected by live/dead cell staining and CCK-8 assay, respectively. ALP and ARS staining was used to evaluate the osteogenic differentiation of BMSCs. Differential gene expression analysis of osteogenic differentiation was performed using high-throughput sequencing. TargetScan database predicted the binding site between miR-19b-3p and WWP1, and a dual-luciferase reporter assay was performed to confirm the targeting binding site. A rat bone defect model was established, and new bone formation was evaluated by Micro-CT, H&E staining, and Masson's trichrome staining. Immunofluorescence staining and immunohistochemistry were used to detect the expression levels of osteogenic-related factors in rat BMSCs. RT-qPCR and Western blot were used to detect the expression levels of genes and proteins in tissues and cells. Result Gel-OCS/MBGN was successfully constructed and loaded with EVs, resulting in Gel-OCS/MBGN@EVs. The in vitro drug release experiment results show that Gel-OCS/MBGN could sustainably release EVs. Further experiments have shown that Gel-OCS/MBGN@EVs could significantly promote the differentiation of BMSCs into osteoblasts. Experiments have shown that WWP1 is a key factor in osteogenic differentiation and is regulated by miR-19b-3p. EVs promote osteogenic differentiation by suppressing WWP1 expression through the transmission of miR-19b-3p. In vivo animal experiments have demonstrated that Gel-OCS/MBGN@EVs significantly promote bone repair in rats with bone defects by regulating the miR-19b-3p/WWP1 signaling axis. Conclusion Functional Gel-OCS/MBGN@EVs were obtained by constructing Gel-OCS/MBGN and loading EVs onto it. EVs could deliver miR-19b-3p to BMSCs, inhibit the expression of WWP1, and promote the osteogenic differentiation of BMSCs, ultimately promoting bone regeneration in rats with bone defects. Graphical Abstract
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Nature Protocols
·
2023
·
IF 14.8
Liu Renmei,Yao Jing,Zhou Siyu,Yang Jing,Zhang Yaqiang,Yang Xiaoyan,Li Leshi,Zhang Yunbin,Zhuang Yingping,Yang Yi,Chen Xianjun
RNA molecules perform various crucial roles in diverse cellular processes, from translating genetic information to decoding the genome, regulating gene expression and catalyzing chemical reactions. RNA-binding proteins (RBPs) play an essential role in regulating the diverse behaviors and functions of RNA in live cells, but techniques for the spatiotemporal control of RBP activities and RNA functions are rarely reported yet highly desirable. We recently reported the development of LicV, a synthetic photoswitchable RBP that can bind to a specific RNA sequence in response to blue light irradiation. LicV has been used successfully for the optogenetic control of RNA localization, splicing, translation and stability, as well as for the photoswitchable regulation of transcription and genomic locus labeling. Compared to classical genetic or pharmacologic perturbations, LicV-based light-switchable effectors have the advantages of large dynamic range between dark and light conditions and submicron and millisecond spatiotemporal resolutions. In this protocol, we provide an easy, efficient and generalizable strategy for engineering photoswitchable RBPs for the spatiotemporal control of RNA metabolism. We also provide a detailed protocol for the conversion of a CRISPR–Cas system to optogenetic control. The protocols typically take 2–3 d, including transfection and results analysis. Most of this protocol is applicable to the development of novel LicV-based photoswitchable effectors for the optogenetic control of other RNA metabolisms and CRISPR–Cas functions.
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MOLECULAR NEUROBIOLOGY
·
2023
·
IF 5.1
Guo Xiying,Lei Min,Ma Guandi,Ouyang Changhan,Yang Xiaosong,Liu Chao,Chen Qingjie,Liu Xiufen
Clinical and epidemiological research shows that people with diabetes mellitus frequently experience diabetic cognitive impairment. Schisandrin A (SchA), one of the lignans found in the dried fruit of Schisandra chinensis, has a variety of pharmacological effects on immune system control, apoptosis suppression, anti-oxidation and anti-inflammation. The goal of the current investigation was to clarify the probable neuro-protective effects of SchA against streptozotocin-induced diabetes deficiencies of the spatial learning and memory in rats. The outcomes show that SchA therapy effectively improved impaired glucose tolerance, fasting blood glucose level and serum insulin level in diabetic rats. Additionally, in the Morris water maze test, diabetic rats showed deficits in spatial learning and memory that were ameliorated by SchA treatment. Moreover, giving diabetic rats SchA reduced damage to the hippocampus structure and increased the production of synaptic proteins. Further research revealed that SchA therapy reduced diabetic-induced hippocampus neuron damage and the generation of Aβ, as demonstrated by the upregulated phosphorylation levels of insulin signaling pathway connected proteins and by the decreased expression levels of inflammatory-related factors. Collectively, these results suggested that SchA could improve diabetes-related impairments in spatial learning and memory, presumably by reducing inflammatory responses and regulating the insulin signaling system.
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JOURNAL OF MATERIALS SCIENCE
·
2023
·Vol. 58
·期 41
·16033-16045
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IF 4.5
Tian Yuhao,Li Ting,Cai Weijie
Recently, bio-ethanol reforming with CO 2 (EDR) has been considered as one of the environment-friendly processes to diminish the emission of greenhouse gas, along with increasing the production of value-added syngas. However, designing high-performance catalysts is still a great challenge. In this work, MOF-derived CoMO x (M:Ce,Zr,Zn) catalysts were applied in EDR reaction for the first time to explore the influence of support types on catalytic performance. Characterization of the investigated catalysts by XPS, XRD, H 2 -TPR, Raman, TEM and BET depicted the difference in cobalt dispersion, specific surface area as well as Co-support interaction. Indeed, H 2 -TPR and XPS results revealed stronger Co-Ce interaction and the smaller particle size of Co 3 O 4 in Co-CeO 2 catalyst which contributed to higher activity. Indeed, 100% of ethanol conversion was obtained at 550 °C and the formed H 2 /CO ratio (1.18) was suitable for the downstream Fischer–Tropsch process. Meanwhile, no remarkable decline of ethanol conversion and product distributions occurred after 40 h stability tests. The findings implied that EDR process might be suitable to consume greenhouse gas and reduce carbon footprint. Characterization results of the used catalysts indicated that Co-CeO 2 catalyst presented an outstanding anti-aggregation and anti-coking property. Herein, the effect of support type in this work might provide guidance for the design of high-performance catalysts for other heterogeneous reactions operated at high temperature. Graphical abstract
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ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
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2023
·
IF 5.8
Wei Huimin,Zhang Lu,Wang Zhi
Co-pollution of antibiotics and heavy metal copper (Cu) is common in freshwater environments because of their wide use as antimicrobial agents, especially in aquaculture. However, the toxic effects of coexisting antibiotics and heavy metals on aquatic plants remain unclear. This study investigated the effect of four antibiotics (i.e., enrofloxacin, ENR; tetracycline, TC; sulfamethoxazole, SMX; erythromycin, ERY), Cu, and their mixture on the growth and physiological responses of Hydrilla verticillata (L.f.) Royle. Results showed that the four antibiotics exhibited toxic effects on the growth and physiological indicators of H. verticillata , and root elongation was the most sensitive endpoint of the phytotoxicity test. The median effect concentration (EC 50 ) of root elongation indicated that TC (EC 50 = 10.05 mg/L) has the highest level of growth toxicity, and the toxicity of ENR to aquatic plants was close to TC (EC 50 = 10.44 mg/L), followed by SMX (EC 50 = 20.08 mg/L). However, there was no significant toxic effect of 20 mg/L ERY on the root elongation. Hydrophobicity may be a key factor affecting the phytotoxicity of antibiotics. Moreover, antagonistic toxic effects were observed under ENR + Cu, TC + Cu, SMX + Cu, and ERY + Cu co-exposures at all the experimental concentrations (0.01–20 mg/L). Due to the concentrations of antibiotics in natural waters usually with ng/L levels, our results suggested that environmental antibiotic concentrations probably pose low ecological risk to aquatic plants and indicated the H. verticillata could be used as phytoremediation candidate to remove antibiotic or antibiotic–Cu pollutions in general nature water.
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CELLULOSE
·
2023
·Vol. 30
·期 17
·11249-11259
·
IF 5.7
Pan Ying,Liang Qianyong,Du Jia,Zhang Huaiwei,Zhang Dong,Zhao Hongting,Lü Ting,Zhang Yan
Boron and nitrogen co-doped carbon dots (BN-CDs) were synthesized using one-pot hydrothermal carbonization. The synthesized CDs were then combined with alginate and polyethyleneimine to create a multilayered coating on cotton fabric through layer-by-layer (LbL) self-assembly. The CD-containing coating emitted visible blue light under a 365 nm UV lamp. The UV protection, thermal stability, flame retardancy, and wash durability of the CD-treated cotton fabric were subsequently investigated. The ultraviolet protection factors of pure cotton fabric and cotton fabric treated with 12 bilayer CD-containing coating were 6.48 and 64.10, respectively. This indicated that the synthesized CD is an efficient UV absorber. In the TGA test, more char residue was observed at 700 °C for CD coated cotton fabrics under a nitrogen atmosphere. After B, N co-doped CDs were enriched in the PEI/alginate multilayered coating, the peak heat release rate (PHRR) of cotton-CDs-12BL was reduced by 15%, and the T PHRR of cotton-CDs-12BL was improved by 26.4 °C in the microscale combustion calorimeter test. These results indicate that B, N co-doped CDs which possess UV absorption and environmental friendliness, have potential application as a flame retardant on the surface of cotton fiber.
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JOURNAL OF PLANT GROWTH REGULATION
·
2023
·
IF 4.8
Islam Md Monirul,Hassan Mahmood Ul,Ishfaq Muhammad,Ripa Farhana Alam,Nadeem Faisal,Ahmad Zeeshan,Xu Jiuliang,Ning Peng,Li Xuexian
Foliar fertilization is an important agricultural practice. The response of crop plants to foliar application of mineral nutrients such as nitrogen (N), phosphorus, and potassium has been well recognized. However, little is known about crop plants’ response to the foliar spray of organic sources of nutrient, in particular, under field conditions. Here, we sprayed glutamine (Gln) at V6, V8, V12, R1 (silk), and R3 (dough) stages of maize hybrid ZD958 to investigate the physiological impacts on grain yield and nutritional quality under sufficient and low-N conditions for two consecutive years. Exogenous application of Gln (1.25 mM) significantly enhanced grain yield approximately by 20.0% under sufficient N and 38.0% under low N, as a combinatorial effect of increases in kernel number and kernel weight. The higher N concentrations in the ear and delaying leaf senescence favor the kernel setting and grain filling, preconditioning higher grain yield at harvest. Further, Gln spray resulted in higher nutritional quality kernels as indicated by higher concentrations of crude proteins, minerals, oil, unsaturated fatty acids (i.e., oleic, and linoleic acids), total carotenoids (i.e., β-carotene, lutein, zeaxanthin, antheraxanthin, and violaxanthin), and α-tocopherol, partially attributing to larger nutrient-rich embryos. Taken together, our findings revealed how Gln promotes maize production and an additional potential to improve grain yield and nutritional quality traits of maize and other closely related cereal crops.
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ISME Journal
·
2023
·Vol. 17
·期 12
·2169-2181
·
IF 11
Wang Xia,Teng Ying,Wang Xiaomi,Xu Yongfeng,Li Ran,Sun Yi,Dai Shixiang,Hu Wenbo,Wang Hongzhe,Li Yanning,Fang Yan,Luo Yongming
Nitrogen is a limiting nutrient for degraders function in hydrocarbon-contaminated environments. Biological nitrogen fixation by diazotrophs is a natural solution for supplying bioavailable nitrogen. Here, we determined whether the diazotroph Azotobacter chroococcum HN can provide nitrogen to the polycyclic aromatic hydrocarbon-degrading bacterium Paracoccus aminovorans HPD-2 and further explored the synergistic interactions that facilitate pyrene degradation in nitrogen-deprived environments. We found that A. chroococcum HN and P. aminovorans HPD-2 grew and degraded pyrene more quickly in co-culture than in monoculture. Surface-enhanced Raman spectroscopy combined with 15 N stable isotope probing (SERS − 15 N SIP) demonstrated that A. chroococcum HN provided nitrogen to P. aminovorans HPD-2. Metabolite analysis and feeding experiments confirmed that cross-feeding occurred between A. chroococcum HN and P. aminovorans HPD-2 during pyrene degradation. Transcriptomic and metabolomic analyses further revealed that co-culture significantly upregulated key pathways such as nitrogen fixation, aromatic compound degradation, protein export, and the TCA cycle in A. chroococcum HN and quorum sensing, aromatic compound degradation and ABC transporters in P. aminovorans HPD-2. Phenotypic and fluorescence in situ hybridization (FISH) assays demonstrated that A. chroococcum HN produced large amounts of biofilm and was located at the bottom of the biofilm in co-culture, whereas P. aminovorans HPD-2 attached to the surface layer and formed a bridge-like structure with A. chroococcum HN. This study demonstrates that distinct syntrophic interactions occur between A. chroococcum HN and P. aminovorans HPD-2 and provides support for their combined use in organic pollutant degradation in nitrogen-deprived environments.
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 10
·1-11
·
IF 5.7
Tian Jiangman,Zhu Qihao,Huang Xiaojing,Li Yuan
A novel sandwich-type “on-off” electrochemiluminescence (ECL) biosensor for the determination of α(2,3)-sial-Gs was designed. Specifically, amino-functionalized porous silica nanoparticles (HPSNs-NH 2 ) were first prepared and then decorated with gold nanoparticles (Au NPs) to form HPSNs-NH 2 @Au NP nanocomposite, which exhibited a strong ability to enhance ECL intensity with K 2 S 2 O 8 as co-reactant (signal-on) and could immobilize the target-specific binding molecules of maackia amurensis lectin (MAL). Additionally, AuPdPt trimetallic nanoparticles were prepared to serve as a quenched ECL signal indicator (signal-off) with the ability of capturing the target non-specific binding molecules of 3-aminophenylboronic acid (APBA) to form a signal label. The sandwich-type ECL biosensor was constructed based on the structure of MAL-α(2,3)-sial-Gs-APBA and achieved a determination toward α(2,3)-sial-Gs with a wide linear range from 1 fg mL −1 to 10 ng mL −1 and a low detection limit of 0.5 fg mL −1 . Furthermore, the proposed ECL biosensor showed satisfactory selectivity, stability, and reproducibility for α(2,3)-sial-Gs determination. Graphical Abstract
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JOURNAL OF NANOBIOTECHNOLOGY
·
2023
·Vol. 21
·期 1
·1-13
·
IF 10.2
Hu Shengyun,Xia Kunkun,Huang Xiaobei,Zhao Ye,Zhang Qingqing,Huang Dongdong,Xu Weiyi,Chen Zhengju,Wang Chenfei,Zhang Zhiyong
Colorectal cancer (CRC) is a major cause of cancer-related deaths in humans, and effective treatments are still needed in clinical practice. Despite significant developments in anticancer drugs and inhibitors, their poor stability, water solubility, and cellular membrane permeability limit their therapeutic efficacy. To address these issues, multifunctional CaCO3 nanoparticles loaded with Curcumin (Cur) and protein deacetylase (HDAC) inhibitor QTX125, and coated with hyaluronic acid (HA) (CaCO3@Cur@QTX125@HA), were prepared through a one-step gas diffusion strategy. Dynamic light scattering (DLS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) showed that CaCO3@Cur@QTX125@HA nanoparticles have uniform spherical morphology and elemental distribution, with diameters around 450 nm and a Zeta potential of − 8.11 mV. The controlled release of Cur from the nanoparticles was observed over time periods of 48 h. Cellular uptake showed that CaCO3@Cur@QTX125@HA nanoparticles were efficiently taken up by cancer cells and significantly inhibited their growth. Importantly, CaCO3@Cur@QTX125@HA nanoparticles showed specific inhibitory effects on CRC cell growth. Encouragingly, CaCO3@Cur@QTX125@HA nanoparticles successfully internalized into CRC patient-derived organoid (PDO) models and induced apoptosis of tumor cells. The multifunctional CaCO3@Cur@QTX125@HA nanoparticles hold promise for the treatment of CRC.
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