WORLD JOURNAL OF GASTROENTEROLOGY
·
2014
·
IF 2.433
Jia-Yi Ma,Run-Hua Li,Kun Huang,Gao Tan,Chen Li,Fa-Chao Zhi
AIM: To investigate the possible role of chitinase 3-like-1 (CHI3L1) in the progression of colitis-associated carcinoma (CAC). METHODS: Thirty-four Balb/c mice were randomly assigned to five groups, including the control, CAC control, CAC + caffeine, colitis control and colitis + caffeine. Three animals were sacrificed every two weeks for blinded macroscopic inspection, histological analysis, and total RNA extraction. An immunofluorescent assay was performed using specimens from the colitis control and colitis + caffeine groups to investigate whether the protective effect of caffeine was associated with less oxidative DNA damage. In vitro , HT29 cells pre-stimulated with different concentrations of recombinant CHI3L1 protein and H 2 O 2 were loaded with the DCFH-DA fluorescent probe to determine the effect of CHI3L1 on intracellular reactive oxygen species production. RESULTS: CHI3L1 mRNA was increased during the progression of colon carcinogenesis. Tumors were mostly located in the distal end of the colon where the expression of CHI3L1 was higher than in the proximal colon. Caffeine-treated mice developed fewer tumors and milder inflammation than untreated mice. CHI3L1 protein increased reactive oxygen species in HT29 cells when exposed to H 2 O 2 . CONCLUSION: Caffeine reduces tumor incidence by decreasing oxidative DNA damage. CHI3L1 may contribute to CAC by increasing reactive oxygen species production.
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REPRODUCTIVE BIOMEDICINE ONLINE
·
2015
·
IF 3.015
Fenju Qin,Jie Zhang,Linsen Zan,Weiqiang Guo,Jin Wang,Lili Chen,Yi Cao,Ouxi Shen,Jian Tong
The aim of the present study was to elucidate whether the GATA-4/SF-1 signalling pathway is involved in the inhibitory effects of melatonin on testosterone production in both the TM3 Leydig cell line and in C57BL/6J mice. In-vitro experiments demonstrated that melatonin treatment significantly reduced testosterone levels in cell culture medium ( P < 0.05 or P < 0.01); and decreased intracellular cyclic adenosine monophospha accumulation ( P < 0.05 or P < 0.01) and mRNA/protein expression of GATA-4 , SF-1 (NR5A1) , StAR , P450SCC (CYP11A1) and 3β-HSD ( P < 0.05 or P < 0.01). These effects were blocked by N-acetyl-2-benzyltryptamin, a melatonin receptor antagonist . Similar effects of melatonin on testosterone production ( P < 0.05 or P < 0.01) and down-regulation of transcription factors GATA-4 and SF-1 ( P < 0.01) were also observed in mice treated with intratesticular injections of melatonin. Overall, the data suggest that the inhibitory effects of melatonin on testosterone production are mediated via down-regulation of GATA-4 and SF-1 expression.
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APPLIED SURFACE SCIENCE
·
2015
·
IF 2.711
Yingjun Li,Qin Wang,Baocang Liu,Jun Zhang
BiOCl nanosheets with tunable lamella thickness and dominantly exposed {0 0 1} facets were selectively synthesized via a facile hydrothermal method. By modifying the synthetic parameters, such as the amount of P123 and mannitol, the reaction time, types of surfactants, the size, thickness, morphologies, and percentage of {0 0 1} facets over BiOCl nanosheets were well controlled. The exposed {0 0 1} facets with high surface energy and high density of oxygen atoms are not only conducive to the adsorption of the rhodamine B (RhB) dye but also can accumulate the photo-generated electrons, which can be captured by O 2 and converted into reactive oxygen species O 2 − . Therefore, the resultant ultrathin BiOCl nanosheets with exposed {0 0 1} facets exhibit superior catalytic activity for dye photosensitization degradation under visible light irradiation. Impressively, the ultrathin BiOCl nanosheets prepared with P123 and mannitol manifest superior catalytic activity and RhB was completely degraded within 20 min. Our current work is expected to offer a new insight into photocatalytic theory for better understanding of visible light photocatalytic reactions and rational design of highly active photocatalysts.
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JOURNAL OF APPLIED POLYMER SCIENCE
·
2015
·Vol. 132
·期 22
·
IF 1.768
Jiaxuan Feng,Hongjun Zang,Qing Yan,Mingguang Li,Xinqiang Jiang,Bowen Cheng
1-Carboxymethyl-3-methylimidazolium hydrochloride ([IMIM–COOH]Cl), a new ionic salt, is proposed as a green, promising solvent for dissolving chitosan. However, because of the optimal dosage of chitosan dissolved in [IMIM–COOH]Cl, a 12 wt % [IMIM–COOH]Cl aqueous solution was selected as an optimum solvent system for dissolving chitosan. The structures of the original and regenerated chitosan were characterized by Fourier transform infrared spectroscopy and X-ray diffraction analysis. Scanning electron microscopy was used to visualize the morphological features of the reconstituted chitosan membranes. Meanwhile, the absorbance, tensile strength, and breaking elongation of the chitosan membranes were measured. The results reveal that 10–11 wt % was an optimal chitosan concentration for preparing membranes. Furthermore, the adsorption capacity for Cu(II) ion of the chitosan membranes was increased with the chitosan concentration decreased from 12 to 8 wt %. © 2015 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2015 , 132 , 41965.
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ANALYTICA CHIMICA ACTA
·
2014
·
IF 4.517
Jialin Qin,Yuanli Li,Chenxiong He,Xiaolan Yang,Yanling Xie,Xiaolei Hu,Chunyan Chen,Lu Wang,Jun Pu,Fei Liao
Homogenous selective assay of albumin (ALB) in clinical sera was tested with 1-anilinonaphthalene-8-sulphonate (ANS) as Förster-resonance-energy-transfer (FRET) acceptor of tryptophan residues and biosensor of ALB. Between the excitation at 280 and 350 nm, the ratio of the fluorescence at 470 nm of free ANS in ethanol was about 1.9 while that of the complexes of ALB and ANS was about 3.9, supporting FRET in complexes of ANS and ALB. ANS below 1.0 mM saturated one site of ALB with K d of about 0.13 μM in 20 mM sodium phosphate buffer at pH 7.0. For selective assay of ALB, 0.30 μM ANS was used to quantify fluorescence of the complexes at 470 nm under the excitation at 280 nm. ALB from 1.8 to 25 nM was quantified, whose lower limit was below 1% than that by bromocresol green assay while one-third than that by immunoturbidimetric assay. Globular proteins at comparable levels gave negligible signals. This new method showed reasonable resistance to other interfering substances in clinical sera. Quantities of ALB in clinical sera by this method were consistent with those by bromocresol green assay and immunoturbidimetric assay. Hence, homogenous assay of ALB with ANS as FRET biosensor was effective.
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JOURNAL OF COLLOID AND INTERFACE SCIENCE
·
2013
·
IF 3.172
Ganggang Chang,Zongbi Bao,Zhiguo Zhang,Huabin Xing,Baogen Su,Yiwen Yang,Qilong Ren
2-Ethyl-1-hexanol has extensive industrial applications in solvent extraction, however, in view of its potential pollution to environment, the removal and recovery of 2-ethyl-1-hexanol is considered an essential step toward its sustainable use in the future. In this work, we report the removal of 2-ethyl-1-hexanol from aqueous solutions containing salts in high concentrations by adsorption on a coal-based activated carbon. Adsorption thermodynamics showed that the experimental isotherms were conformed well to the Langmuir equation. Also it was found that inorganic salts, i.e. MgCl 2 and CaCl 2 in high concentration significantly enhanced the adsorption capacity from 223 mg/g in the deionized water to 277 mg/g in a saline water. This phenomenon of adsorption enhancement could be ascribed to the salt-out effect. Kinetic analysis indicated that adsorption kinetics follows the pseudo-second-order equation and the adsorption rate constants increase with the salt concentration. The dynamic breakthrough volume and adsorbed amount of 2-ethyl-1-hexanol were significantly elevated when the salt is present in the water. The dynamic saturated adsorption amount increased from 218.3 mg/g in the deionized water to 309.5 mg/g in a salt lake brine. The Tomas model was well applied to predict the breakthrough curves and determine the characteristics parameters of the adsorption column.
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PLoS One
·
2013
·Vol. 8
·期 8
·e70565
·
IF 3.73
Luyan Guo,Yanyan Xing,Rui Pan,Mingliang Jiang,Zheng Gong,Liqing Lin,Junbing Wang,Guoyin Xiong,Jun Dong
Curcumin is a molecule found in turmeric root that has anti-inflammatory, antioxidant, and anti-tumor properties and has been widely used as both an herbal drug and a food additive to treat or prevent neurodegenerative diseases. To explore whether curcumin is able to ameliorate HIV-1-associated neurotoxicity, we treated a murine microglial cell line (N9) and primary rat cortical neurons with curcumin in the presence or absence of neurotoxic HIV-1 gp120 (V3 loop) protein. We found that HIV-1 gp120 profoundly induced N9 cells to produce reactive oxygen species (ROS), tumor necrosis factor-α (TNF-α) and monocyte chemoattractant protein-1 (MCP-1). HIV-1 gp120 also induced apoptosis of primary rat cortical neurons. Curcumin exerted a powerful inhibitory effect against HIV-1 gp120-induced neuronal damage, reducing the production of ROS, TNF-α and MCP-1 by N9 cells and inhibiting apoptosis of primary rat cortical neurons. Curcumin may exert its biological activities through inhibition of the delayed rectification and transient outward potassium (K + ) current, as curcumin effectively reduced HIV-1 gp120-mediated elevation of the delayed rectification and transient outward K + channel current in neurons. We conclude that HIV-1 gp120 increases ROS, TNF-α and MCP-1 production in microglia, and induces cortical neuron apoptosis by affecting the delayed rectification and transient outward K + channel current. Curcumin reduces production of ROS and inflammatory mediators in HIV-1-gp120-stimulated microglia, and protects cortical neurons against HIV-1-mediated apoptosis, most likely through inhibition of HIV-1 gp120-induced elevation of the delayed rectification and transient outward K + current.
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International Journal of Environmental Research and Public Health
·
2013
·Vol. 10
·期 7
·2897-2905
·
IF 1.998
Juan Peng,Shanshan Song,Liguang Xu,Wei Ma,Liqiang Liu,Hua Kuang,Chuanlai Xu
We have established a highly sensitive sandwich enzyme-linked immunosorbent assay (ELISA) based on two monoclonal antibodies (mAb) to measure the content of the major peanut allergen Ara h 1 in foods. Two mAbs were selected out of 12 murine hybridoma cells secreting Ara h 1-specific antibody. Using mAb 6 as the capture antibody and HRP-labelled mAb 4 as the detection antibody, the limit of detection (LOD) the assay was 0.34 ng/mL. Cross-reaction analysis showed that this method was strongly specific and had no cross-reactions with Ara h 2, pea protein or soy protein. Sample analysis showed that this ELISA was a useful tool to monitor peanut allergens in food products by measuring Ara h 1 content.Keywords:peanut allergen;Ara h 1;monoclonal antibody;sandwich ELISA
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2015
·
IF 2.353
Renping Cao,Tao Liang,Wensheng Li,Chunyan Cao,Yufeng Wen,Qianglin Hu
Sr [1−3( x + y )/2] Al 2 B 2 O 7 : x Eu 3+ , y Bi 3+ ( x = 0–5 mol% and y = 0–5 mol%) phosphors are synthesized by a solid-state reaction method in air, and their crystal structure, fluorescence lifetime, and luminescence properties are investigated. The optimal composition is determined to be (Sr 0.94 Eu 0.03 Bi 0.01 )Al 2 B 2 O 7 . The PLE band peaks within the range 200–550 nm are due to O 2− → Eu 3+ charge transfer band, 7 F 0 → 5 H 3 , 5 D 4 , 5 L 7 , 5 L 6 , 5 D 3 , 5 D 2 , and 5 D 1 transitions, respectively. The strongest PL band peak under excitation 394 nm light is at ∼615 nm owing to 5 D 0 → 7 F 2 transition of Eu 3+ ion. The PL intensity of Eu 3+ , Bi 3+ co-doped SrAl 2 B 2 O 7 phosphor is 1.3 times that of Eu 3+ doped SrAl 2 B 2 O 7 phosphor due to the energy transfer between Eu 3+ and Bi 3+ ions, which is explained by the energy level diagrams of Bi 3+ and Eu 3+ ions. The CIE chromaticity coordinates of Sr 0.955 Al 2 B 2 O 7 :0.03Eu 3+ and Sr 0.94 Al 2 B 2 O 7 :0.03Eu 3+ , 0.01Bi 3+ phosphors under excitation 394 nm light are ( x = 0.6292, y = 0.3702) and ( x = 0.6284, y = 0.3711), respectively. These phosphors will be used as reddish orange emitting phosphor candidate for white LED with ∼394 nm near ultraviolet LED chip.
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Soft Matter
·
2015
·Vol. 11
·期 31
·6266-6274
·
IF 4.029
Chen Chen,Yue-Cheng Qian,Chuan-bin Sun,Xiao-Jun Huang
The amphiphilic random copolymer poly(β-D-glucose-co-1-octyl)phosphazene (PGOP) can undergo continuous morphological transitions in DMF–water mixed solvents. In this study, the ratio of glucose moieties to octyl moieties was controlled via a two-step thiol–ene reaction. As a result, polyphosphazenes with glycosyl functionalization degrees of 58.1% (PGOP-1), 74.1% (PGOP-2) and 87.0% (PGOP-3) were obtained. These amphiphilic polyphosphazenes self-assemble in both water and water–DMF mixtures. Several self-assembled morphologies including spheres, rods and vesicles were formed though careful control of the water content (WC) in the DMF solvent as well as of the hydrophilicity or hydrophobicity of the copolymers. We also found that an increase in the hydrophobic proportion led to faster morphological transitions at a constant WC. The thermodynamics of micellization were also studied by Isothermal Titration Calorimetry (ITC), and the strong hydrophobic interactions in PGOP-1 were demonstrated by their highly exothermic nature. These self-assemblies have potential applications in biosensing, lectin adsorption and drug loading with controlled release.
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Nano Research
·
2016
·Vol. 9
·期 3
·787-799
·
IF 8.893
Yang Zhongzhen,Ding Xianguang,Jiang Jiang
Nanocomposites combining magnetic and plasmonic components have received widespread attention in recent years due to their potential applications in biomedical research. Herein, we describe a facile method for growing small iron oxide nanoparticles on various plasmonic core materials with different shapes and surfaces by utilizing a polypyrrole interlayer. By focusing on Au nanorod@polypyrrole@iron oxide (Au NR@PPy@Fe
x
O) nanocomposites, we show that these systems exhibit a low r
2 / r
1 ratio of 4.8, making them efficient T
1 positive contrast-enhancing agents for magnetic resonance imaging (MRI). Moreover, we show that the nanocomposites are excellent photothermal agents in the second near infrared region, with high photothermal conversion efficiency, reaching up to 46%. In addition, the Au NR@PPy@Fe
x
O nanocomposites show very low cytotoxicity. In summary, the present results highlight the great potential of the synthetic method and the nanocomposites developed in this study for T
1 MRI and/or infrared thermal imaging-guided photothermal cancer therapeutic applications.
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PHYSICAL CHEMISTRY CHEMICAL PHYSICS
·
2016
·Vol. 18
·期 19
·13554-13563
·
IF 4.449
Yanmin Tao,Yongjun Hu,Weizhan Xiao,Jiwen Guan,Fuyi Liu,Xiaobin Shan,Liusi Sheng
Photoionization and dissociation of the 1-propanol dimer and subsequent fragmentations have been investigated by synchrotron vacuum ultraviolet (VUV) photoionization mass spectrometry and theoretical calculations. Besides the protonated monomer cation (C3H7OH)·H+ (m/z = 61) and Cα–Cβ bond cleavage fragment CH2O·(C3H7OH)H+ (m/z = 91), the measured mass spectrum at an incident photon energy of 13 eV suggests a new dissociation channel resulting in the formation of the (C3H7OH)·H+·(C2H5OH) (m/z = 107) fragment. The appearance energies of the fragments (C3H7OH)·H+, CH2O·(C3H7OH)H+ and (C3H7OH)·H+·(C2H5OH) are measured at 10.05 ± 0.05 eV, 9.48 ± 0.05 eV, and 12.8 ± 0.1 eV, respectively, by scanning photoionization efficiency (PIE) spectra. The 1-propanol ion fragments as a function of VUV photon energy were interpreted with the aid of theoretical calculations. In addition to O–H and Cα–Cβ bond cleavage, a new dissociation channel related to Cβ–Cγ bond cleavage opens. In this channel, molecular rearrangement (proton transfer and hydrogen transfer after surmounting an energy barrier) gives rise to the generated complex, which then dissociates to produce the mixed propanol/ethanol proton bound cation (C3H7OH)·H+·(C2H5OH). This new dissociation channel has not been reported in previous studies of ethanol and acetic acid dimers. The photoionization and dissociation processes of the 1-propanol dimer are described in the photon energy range of 9–15 eV.
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