International Journal of Electrochemical Science
·
2023
·
IF 1.5
Zhentao Li,Yanhui Fu,Qingsheng Zhu,Saijin Wei,Jie Gao,Yifu Zhu,Ting Xue,Ling Bai,Yangping Wen
Phosphorene (BP) is a rapidly rising star in two-dimensional (2D) layered nanomaterials beyond graphene, and its urgent issues of ambient instability still have to be resolved. Here, the bimetallic Pt-Pd nanoparticle on the BP surface was employed as multifunctional chemical and biochemical sensing platform for electrocatalytic detection of p -aminophenol (PAP), β-galactosidase (β-Gal), and Escherichia coli ( E. coli ). The BP-supported Pt-Pd nanoelectrocatalyst obtained by the electrodeposition of bimetallic Pt-Pd nanoparticle onto the BP surface was very stable in aqueous buffer containing oxygen, which displayed good voltammetric responses for PAP in a linear range of 0.02 -5 μmol·L -1 with a detection limit (LOD) of 1.41 × 10 −8 M and sensitivity 115.81 μA·μM -1 ·cm -2 . PAP could be obtained by the biochemical reaction of β-Gal in the presence of substrate PAP-β-galactopyranoside, which could realize for biochemical sensing of β-Gal in a linear range of 0.2 -0.8 mU·L -1 with a LOD of 9.35 nU·L -1 and sensitivity of 4.77 μA (mU·L -1 ) -1 ·cm -2 . The β-Gal is an indicator of total coliforms represented by E. coli that are induced by isopropyl-β-thiogalactopyranoside, which could applied for biochemical sensing of E. coli in a linear range of 6 × 106 –1.6 × 108 cfu·mL-1 with LOD of 6.65 × 10 5 cfu·mL -1 and sensitivity of 2.44 μA·(cfu·mL -1 ) -1 ·cm -2 . This work will provide experimental support for multifunctional chemical and biochemical sensing platform based on 2D layered nanomaterials decorated with bimetallic nanoelectrocatalysts.
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SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
·
2023
·
IF 4.4
Ye Li,Hongrui Liu,Chunyu Mu,Jiali Gu,Chun Li
Ethoxyquin (EQ) is a synthetic antioxidant that is derived from quinolines and found in many meat products. EQ is strictly regulated in feed due to its potential health implications. An investigation of the interaction mechanism between EQ and transporter protein before and after β-cyclodextrin (β-CD) encapsulation was conducted with the use of multi-spectroscopy, cyclic voltammetry, and molecular docking. EQ formed complexes with bovine serum albumin (BSA), and affected secondary structure and microenvironment polarity of BSA. However, at 298K, EQ's fluorescence quenching constants decreased from (9.81±0.05)×10 3 L mol -1 to (4.94±0.09)×10 3 L mol -1 , binding constants decreased from (10.28±0.02)×10 3 L mol -1 to (2.08±0.07)×10 3 L mol -1 , after encapsulation in β-CD as well as the binding distance increased. β-CD contains part of EQ in its hydrophobic cavity, inhibiting its binding to BSA. β-CD inclusion complex prevented adverse effects of EQ on BSA conformation. However, β-CD encapsulation had no effect on EQ's antioxidant activity.
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EUROPEAN POLYMER JOURNAL
·
2022
·
IF 5.546
Zhi Li,Lifen Zhang,Changning Xue,Yuman Zhang,Yao Yu,Xinhong Guo,Zhenzhong Zhang
Hydroxypropyl-β-Cyclodextrin (HP-β-CD) was used to form the HP-β-CD/Oridonin inclusion complex. Then, the novel Multi-loaded nanovesicles systems (NVs) were established to encapsulate both hydrophilic (Trehalose) and hydrophobic drugs (Oridonin inclusion complex) with peptides. NVs had an average size of 224.4 ± 10.84 nm and a zeta potential of 17.83 ± 1.05 mV. Furthermore, these NVs significantly decreased lipid over-accumulation, and reverse the formation of foam cells in RAW264.7 cells. Oridonin and Trehalose in NVs provided significant synergistic effects to inhibit lipid over-accumulation (CI < 1). In addition, NVs could modulate the release of IL-1β, IL-6 and TNF-α, promote the formation of autophagosomes. Taken together, the Multi-loaded NVs may be used as an effective drug delivery system, providing new insights into decrease the formation of foam cells and regulate the inflammation.
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COLLOIDS AND SURFACES B-BIOINTERFACES
·
2022
·
IF 5.999
Huiyan Wang,Jie Li,Han Liang,Xinrong Huang,Na Meng,Ninglin Zhou
In this work, an effective method was explored to prepare silver nanoparticles/graphene oxide-sulfobutylether-β-cyclodextrin (AgNPs/GO-SBE-β-CD) antibacterial nanocomposite . Dopamine (DA) was used as a green and mild reductant to directly in situ reduce silver nitrate on Sulfobutylether-β-cyclodextrin (SBE-β-CD) modified graphene oxide (GO-SBE-β-CD). Its synthesized, antibacterial properties and biocompatibility were studied. Results showed that silver nanoparticles with an average size of 73 nm were uniformly distributed on the GO-SBE-β-CD sheets. In addition, AgNPs/GO-SBE-β-CD were proved an effective antibacterial material against both gram-negative and gram-positive, reduced the viability of E. coli and S. aureus by 90 % at 40 µg/mL. At the same time, AgNPs/GO-SBE-β-CD co-cultured with L929 cells did not show cytotoxicity. In vivo experiment, AgNPs/GO-SBE-β-CD showed good biocompatibility. Collectively, AgNPs/GO-SBE-β-CD is a promising material to prevent bacterial infection, expecting to be applied in wound healing.
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JOURNAL OF ALLOYS AND COMPOUNDS
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2022
·
IF 6.371
Wenhui Zhu,Ali Reza Kamali
Metastable monoclinic molybdenum trioxide (β-MoO 3 ) combined with perfect hexagonally-shaped MoS 2 and graphene nanosheets are fabricated by facile mechanochemical-molten salt treatment of irregularly-shaped molybdenum disulfide (MoS 2 ) and graphite in air. This hybrid nanostructure exhibits an excellent Li-ion storage performance, outperforming the nanocomposites made of conventional orthorhombic α-MoO 3 . To this end, ball-milled MoS 2 and graphite are treated in the presence of sodium chloride (NaCl) at 900–1200 °C, leading to the preparation of MoS 2 /MoO x @graphene (MSO@G) nanocomposites in an easily scalable and affordable way. The formation of β-MoO 3 is realized at 1100 °C (MSO@G-1100), outperforming the electrochemical performances of other samples and the initial MoS 2 . Moreover, the influence of binder on the electrochemical performance of MSO@G-1100 is studied, based on which polystyrene acrylic-acrylate (SAE) is found to provide a considerably low charge transfer resistance (27.59 Ω), outperforming conventional binders. The electrode exhibits a reversible Li-ion storage capacity of 878 mAh g −1 after 245 cycles at the current density of 100 mA g −1 . The capacitive contribution to the total Li-ion energy storage performance of MSO@G-1100/SAE is found to be significant with the value of around 62% at the potential scan rate of 0.3 mV s −1 . This article report on the facile, clean and sustainable preparation of β-MoO 3 based nanocomposites with remarkable electrochemical performance.
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Food Science and Human Wellness
·
2022
·
IF 8.022
Zhixian Li,Zhuqing Dai,Enjuan Shi,Peng Wan,Guijie Chen,Zhongyuan Zhang,Yayuan Xu,Ruichang Gao,Xiaoxiong Zeng,Dajing Li
β -Carotene, a typical non-oxygenated carotenoid, is the most efficient source of retinol (VA). The low bio-availability of β -carotene lead to large accumulation in colon; however, the relationship between β -carotene and gut microflora remains unclear. This study intends to explore the interaction between β -carotene and gut microflora using an in vitro fermentation model. After 24 h fermentation, the degradation rate of β -carotene was (64.28 ± 6.23)%, which was 1.46 times that of the group without gut microflora. Meanwhile, the production of VA was nearly 2 times that of the group without gut microflora, indicating that the gut microflora can metabolize β -carotene into VA. β -Carotene also influences the production of short-chain fatty acids (SCFAs), the production of total SCFAs in 0.5 mg/mL β -carotene (BCM) group was (44.00 ± 1.16) mmol/L, which was 2.26 times that of the blank control (BLK) group. Among them, the production of acetic acid in BCM group was (19.06 ± 0.82) mmol/L, which was 2.64 time that of the BLK group. Furthermore, β -carotene significantly affected the structure and composition of gut microflora, increasing the abundance of Roseburia , Parasutterella and Lachnospiraceae, and decreasing the abundance of Dialister , Collinsella and Enterobacter ( P < 0.05). This study provides a new way to understand how β -carotene works in human body with gut microflora.
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MICROCHEMICAL JOURNAL
·
2022
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IF 5.304
Jinmei Luo,Shuhuai Li,Yuwei Wu,Chaohai Pang,Xionghui Ma,Mingyue Wang,Chenghui Zhang,Xu Zhi,Bei Li
The key to improving the sensitivity of electrochemical sensors for direct detection is the introduction of catalytically amplified nanomaterials. Accordingly, in this study, a new strategy for the preparation of electrochemical sensors with multiple amplification effects is proposed. Graphene oxide (GO), gold nanoparticles (Au NPs), and β-cyclodextrin (β-CD) were introduced to the surface of a glassy carbon electrode via cyclic voltammetry to prepare a modified electrode sensor with a GO/Au NPs/β-CD thin film. The prepared sensor effectively catalyzed and amplified the reduction current of imidacloprid in a B-R buffer with pH = 5.0 owning to the multiple amplification effects of GO/Au NPs/β-CD. A good linear relationship was observed the level of the reduction current response and the imidacloprid at concentrations ranging from approximately 5 × 10 −10 to 3000 × 10 −10 mol/L; thus, a new imidacloprid detection method using an electrochemical sensor was established. The new method, whose detection limit reached 1.33 × 10 −10 mol/L, was tested on actual samples, achieving recoveries of 92.0 % to 110.0 %.
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JOURNAL OF RARE EARTHS
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2022
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IF 4.632
Junlian Wang,Wen Xu,Jianguo Cui,Guodong Xu,Yufu Chen,Peilong Wang,Ziyong Chang
Heavy rare earth element (HREE) separation has always been a great challenge. The kind of dialkylphosphinic acids is recognized as the third generation of organophosphorous acid extractants. However, detailed relationship between β -substituent (especially when it changes from H to methyl, and further to ethyl) and their extraction behaviors and selectivity for HREEs have rarely been reported. Besides, there are also few reports on effect of γ , δ -substituent on HREE extraction and separation. In this paper, we synthesized five and collected two dialkylphosphinic acids with different β , γ , δ -substituents (from H to ethyl). These extractants were divided into two groups to discuss their extraction behaviors and selectivity for HREEs: β -substituent group (P208, INET-1, P218, USTB-1 and P227) and γ , δ -substituent group (P218, P2132 and Cyanex 272). To evaluate and compare their extractabilities, P507 was used as a reference and Tm, Yb, Lu were extracted with 0.01 mol/L of these extractants from single Tm, Yb and Lu solutions (∼4 × 10 −4 mol/L) at different initial pH. Besides, pH 0.5 Tm , pH 0.5 Yb and pH 0.5 Lu values and their differences (ΔpH 0.5 Tm–Yb , ΔpH 0.5 Yb–Lu , ΔpH 0.5 Tm–Lu ) for each extractant were given to roughly evaluate their separation performance for Tm, Yb and Lu. To further investigate the selectivity of these extractants for HREEs, Tb, Dy, Ho, Er, Tm, Yb and Lu were extracted from their mixed solution with initial pH of 4.00 at phase ratio A/O of 2:1, 1:1 or 1:2. Their HREE separation performance is mainly discussed based on the adjacent HREE separation factors ( β N+1/N ) and their average value ( β T b − L u ¯ ).
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PSYCHOPHARMACOLOGY
·
2022
·Vol. 239
·期 11
·3579-3593
·
IF 4.415
Mou Chen-Ye,Xie Yan-Fei,Wei Jia-Xin,Wang Qi-Yao,Le Jing-Yang,Bao Yong-Jie,Zhang Pan-Pan,Mao Yue-Chun,Huang Xing-Han,Pan Han-Bo,Naman C. Benjamin,Liu Lin,Liang Hong-Ze,Wu Xiang,Xu Jia,Cui Wei
Rationale
Tau hyperphosphorylation and aggregation is considered as a main pathological mechanism underlying Alzheimer’s disease (AD). Rose Bengal (RB) is a synthetic dye used for disease diagnosis, which was reported to inhibit tau toxicity via inhibiting tau aggregation in Drosophila . However, it was unknown if RB could produce anti-AD effects in rodents. Objectives The research aimed to investigate if and how RB could prevent β-amyloid (Aβ) oligomers-induced tau hyperphosphorylation in rodents. Methods and results RB was tested in vitro (0.3–1 μM) and prevented Aβ oligomers-induced tau hyperphosphorylation in PC12 cells. Moreover, RB (10–30 mg/kg, i.p. ) effectively attenuated cognitive impairments induced by Aβ oligomers in mice. Western blotting analysis demonstrated that RB significantly increased the expression of pSer473-Akt, pSer9-glycogen synthase kinase-3β (GSK3β) and reduced the expression of cyclin-dependent kinase 5 (CDK5) both in vitro and in vivo. Molecular docking analysis suggested that RB might directly interact with GSK3β and CDK5 by acting on ATP binding sites. Gene Ontology enrichment analysis indicated that RB might act on protein phosphorylation pathways to inhibit tau hyperphosphorylation. Conclusions RB was shown to inhibit tau neurotoxicity at least partially via inhibiting the activity of GSK3β and CDK5, which is a novel neuroprotective mechanism besides the inhibition of tau aggregation. As tau hyperphosphorylation is an important target for AD therapy, this study also provided support for investigating the drug repurposing of RB as an anti-AD drug candidate.
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INTERNATIONAL IMMUNOPHARMACOLOGY
·
2022
·
IF 5.714
Xin Chen,Sai Zhu,Si-Yu Chen,Jia-Nan Wang,Li-Jiao Sun,Shan-Min Tao,Xiao-Feng Li,Hai-Di Li,Ying-Yin Sun,Chuan-Hui Xu,Xiao-Guo Suo,Ming-Lu Ji,Cheng Huang,Xiao-Ming Meng,Jun Li
Hepatic fibrosis is an essential pathology of multiple chronic liver diseases. The aim of this study was to investigate the role of miR-301a-3p in hepatic fibrosis. We found that miR-301a-3p was upregulated in hepatic fibrosis patients and in culture-activated human hepatic stellate cells (HSCs). Interestingly, miR-301a-3p expression was increased in hepatic fibrosis progression mice while decreased in hepatic fibrosis recovery mice, indicating that miR-301a-3p may participate in the hepatic fibrosis pathology. Functionally, the effects of miR-301a-3p both on hepatic fibrosis progression and regression were assessed in vivo . Inhibiting miR-301a-3p ameliorated mouse liver fibrogenesis and collagen deposition and suppressed HSC activation and fibrogenic factor expression. Whereas, in hepatic fibrosis regression, upregulating miR-301a-3p impaired mouse hepatic fibrosis recovery by inducing HSC activation and triggering inflammation. Consistently, gain-of-function and loss-of-function analysis of miR-301a-3p were performed to evaluate its effects on human HSCs LX-2 cell. We found that suppressing miR-301a-3p inhibited LX-2 cell activation and proliferation, and induced LX-2 cell apoptosis, accompanied by decreased fibrotic mediators expression. Collectively, these findings suggest miR-301a-3p drives liver fibrogenesis and HSC activation in hepatic fibrosis. Mechanistically, we demonstrated miR-301a-3p binds directly to phosphatase and tensin homolog (PTEN) by luciferase reporter analysis, pull-down, and RIP assay. Indicating that miR-301a-3p plays a critical role in promoting liver fibrogenesis via modulating the PTEN/platelet derived growth factor β (PDGFR-β) pathway. In conclusion, our findings demonstrate that miR-301a-3p expression is closely correlated with hepatic fibrosis pathology, and that enhancing miR-301a-3p maintains the HSC profibrogenic phenotype, triggers inflammatory responses, promotes fibrogenic factor production, and further exacerbates liver fibrogenesis. These findings suggest that miR-301a-3p may serve as a promising diagnostic and prognosis biomarker for hepatic fibrosis treatment.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2022
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IF 5.895
Haiyun Li,Yachun Ma,Tuo Yao,Li Ma,Jiangui Zhang,Changning Li
In the process of microbial degradation of pyrethroid pesticides, the synergistic effect of the microbial community is more conducive to the complete degradation of toxic compounds than a single strain. At present, the degradation pathway of pyrethroids in a single strain has been well revealed, but the synergistic metabolism at the community level has not been well explained. This study elucidated the bacterial community succession, metabolic pathway, and phytotoxicity assessment during β-cyfluthrin biodegradation by a novel bacterial consortium enriched from contaminated soil. The results showed that the half-life of β-cyfluthrin at different initial concentrations of 0.25, 0.5, 0.75, and 1.0 mg mL–1 were 4.16, 7.34, 12.81, and 22.73 days, respectively. Enterobacter was involved in β-cyfluthrin degradation metabolism in the initial stage, and other bacterial genera (Microbacterium, Ochrobactrum, Pseudomonas, Hyphomicrobiaceae, Achromobacter, etc.) significantly contribute to the degradation of intermediate metabolites in the later stages. Functional gene prediction and metabolite analysis showed that xenobiotic biodegradation and metabolism, especially benzoate degradation and metabolism by cytochrome P450 were the major means of β-cyfluthrin degradation. Further, two degradation pathways of β-cyfluthrin were proposed, which were mainly ester hydrolysis and oxidation to degrade β-cyfluthrin through the production of carboxylesterase and oxidoreductase. In addition, the inoculated bacterial consortium could degrade β-cyfluthrin residues in water and soil and reduce its phytotoxicity in Medicago sativa. Hence, this novel bacterial consortium has important application in the remediation environments polluted by β-cyfluthrin.
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JOURNAL OF CHROMATOGRAPHIC SCIENCE
·
2022
·Vol. 61
·期 7
·625-636
·
IF 1.555
Zhong Hui,Zhang Tianci,Zeng Qingli,Li Laisheng
An azobenzenediamide bridged bis(β-cyclodextrin) chiral stationary phase (AZCDP) was prepared, and its high-performance liquid chromatography performance in reversed-phase and polar organic modes was evaluated by chiral probes, including triazoles, flavanones, amino acids and β-blockers. The results showed that AZCDP had strong chiral separation ability and the 40 chiral compounds were successfully resolved, of which 32 were completely separated (Rs ≥ 1.5) and the best enantioresolution was up to 3.93 within 20 min under a wide range of pH value and temperature. The separation ability of AZCDP with double cavities was significantly better than common CD-CSPs with single cavity, which was related to the synergistic inclusion effect. Compared with the previously reported stilbene (C=C)-bridged CSP, AZCDP with azobenzene (N=N)-bridged had a wider resolution range. For example, it could resolve myclobutanil, pindolol, carteolol, betaxolol, bevanolol and bitertanol, which could not be resolved before, and should be related to the fact that the flexible N=N was more compatible with the synergistic inclusion between cavities than the rigid C=C bridge group. The azobenzenediamide bridging group could also provide hydrogen bond, π–π and other sites, which was conducive to chiral separations.
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