APPLIED CLAY SCIENCE
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2019
·
IF 3.89
Shangying Li,Hongping He,Qi Tao,Yunfei Xi,Aiqing Chen,Shichao Ji,Chaoqun Zhang,Yiping Yang,Jianxi Zhu
In this study, beidellite , paragonite and other 2:1 type layered aluminosilicates with high layer charges (2:1 type LA-HLC) were synthesized under hydrothermal conditions by using boehmite as a precursor. The concentrations of dissolved Al from boehmite precursor had important effects on the mineral components of the hydrothermal products and the layer charge density of the resultant minerals. A combination of 2:1 type LA-HLC, paragonite, and minor beidellite formed at a relatively high concentration of dissolved Al, while beidellite with a small amount of 2:1 type LA-HLC was produced at a low concentration of dissolved Al. The resultant 2:1 type LA-HLC exhibited poor swelling ability when treated with ethylene glycol. A solid-state transformation mechanism was proposed for the transformation based on the HRTEM images and EDS analyses, which showed that lattice fringes representing (001) faces of 2:1 type layered aluminosilicates (2:1 type LA) were parallel to the (020) direction of boehmite. The successful transformation of boehmite into 2:1 type LA provides insights to well understand the formation of 2:1 type LA in geological processes and offer a facile preparation approach for the synthesis of 2:1 type LA with different layer charges.
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Frontiers in Microbiology
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2019
·
IF 4.259
Wu Hao,Guo Ye,Chen Lei,Chen Guiguang,Liang Zhiqun
This study characterized the biosynthetic pathway of the secondary metabolite 1-deoxynojirimycin (DNJ) from Streptomyces lavendulae. The results revealed that glucose was a preferable precursor for DNJ synthesis, and its carbon skeleton underwent a C2-N-C6 cyclization reaction during synthesis. The biosynthetic pathway was related to the glycolysis pathway, and started from fructose-6-phosphate, and involved amination, dephosphorylation, oxidation, cyclization, dehydration, and reduction reaction steps, yielding DNJ. Then, based on clarified biosynthetic pathway information, precursors, analogs, and metabolism inhibitors were used as novel regulators to enhance the production of DNJ. The results demonstrated that the titer of DNJ could reach 296.56 mg/L, which was 3.3-fold higher than that of a control group (90 mg/L) when sodium citrate (0 h, 5 g/L), sorbose (0 h, 1 g/L), iodoacetic acid (20 h, 50 mg/L), and glucose (26 h, 7 g/L) were added during the fermentation process. This study provides a new understanding of the biosynthetic pathway of DNJ, and also provides an efficient strategy to regulate the production of DNJ based on this biosynthetic pathway, which is a new perspective for the regulation of other secondary metabolites.
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Bioengineered
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2018
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IF 1.639
Yanshun Kang,Xiaolin Xu,Huiran Pan,Jing Tian,Weihua Tang,Siqi Liu
In this report, the decolorization features of extracellular enzymes and mycelia separately prepared from Aspergillus sp. TS-A CGMCC 12,964 (120 h) were investigated. The fermentation broth of TS-A degraded 98.6% of Mordant Yellow 1 (50 mg/L) at an initial pH 6 within 1 h with over 70% of the dye (50 mg/L) degraded by extracellular enzymes and 18.8% removed by live mycelia. The degradation products of the dye were analyzed by UV-Vis and FTIR spectra. The decolorization rates of extracellular enzymes and mycelia were examined under different contact periods, dye concentrations and pH values. The extracellular enzymes exhibited excellent degradation activity under weak acidic conditions. In addition, biosorption models of mycelia fitted well the Langmuir isotherm model and the pseudo-second-order kinetic equation. Although the decolorization process was achieved through the synergistic effects of mycelia and extracellular enzymes, decolorization was dominated by the biodegradation activity of the extracellular enzymes from TS-A.
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Environmental Science & Technology Letters
·
2017
·
IF 5.308
Jun-Cheng Han,Guo-Jun Chen,Li-Ping Qin,Yang Mu
Cr isotope fractionation during microbial reduction processes is commonly recognized as a promising tool in biogeochemistry and bioremediation. However, the mechanism of Cr isotope fractionation during microbial reduction is poorly understood. In this work, the relationship between the bacterial respiratory pathway and Cr isotope fractionation was investigated in Shewanella oneidensis MR-1. For comparison with the wild type, a mutant strain (ΔomcA/ΔmtrC) with a deficiency in extracellular Cr(VI) reduction was constructed by deleting the omcA and mtrC genes, which encode the terminal reductase during extracellular reduction. The magnitudes of Cr isotope fractionation (ε) for Cr(VI) reduction by the wild type and ΔomcA/ΔmtrC were −2.42 ± 0.68‰ and −2.70 ± 0.22‰, respectively. Surprisingly, the ε values were not significantly different between the two strains. This suggests that isotope fractionation is independent of the metal respiratory pathway during Cr(VI) reduction by S. oneidensis MR-1. Moreover, a three-step Cr isotope fractionation model that includes uptake, reduction, and efflux was proposed to exist during intracellular Cr(VI) reduction by S. oneidensis MR-1. The developed model provides a better understanding of Cr isotope fractionation during microbial reduction.
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Surface Topography-Metrology and Properties
·
2017
·Vol. 5
·期 4
·044001
·
IF 0
Xiangyu Li,Yuwei Ye,Tong Liu,Wenru Zheng,Feng Yang,Haichao Zhao,Liping Wang
Novel quaternary ammonium cation containing tetraaniline (QATA) was successfully synthesized by condensation of amine-capped tetraaniline with 6-bromohexanoic acid, followed by quaternarization with triethylamine. The corrosion inhibition performance of QATA with their adsorption mechanisms for Q235 steel was studied in 1 M HCl solution by a series of methods such as weight loss measurements, potentiodynamic polarization, electrochemical impedance spectroscopy, scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS) and atomic force microscopy (AFM). The inhibition efficiency (IE%) increased with increasing concentrations of QATA, reaching a value up to 97.47% at a concentration of 150 mg l−1. Potentiodynamic polarization curves showed that the QATA affected both cathodic and anodic protection and was a mixed type inhibitor in 1 M HCl corrosive medium. Adsorption isotherm studies confirmed that the absorption of QATA on the Q235 steel surface in 1 M HCl solution obeyed Langmuir adsorption isotherm, and the adsorption process of corrosion inhibition on Q235 steel surface involved both the physical and chemical adsorption. The EDS analysis determined the adsorption of QATA molecules on the steel surface, the surface morphologies before and after immersion in 1 M HCl medium were also investigated by SEM and AFM.
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EXPERIMENTAL CELL RESEARCH
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2017
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IF 3.546
Yong Yang,Zhiyu Xi,Yuan Xue,Jie Ren,Yuhao Sun,Baofeng Wang,Zhihong Zhong,Guo-yuan Yang,Qingfang Sun,Liuguan Bian
Oxidative stress mediated secondary injury contributes to neurological deterioration after intracerebral hemorrhage (ICH). Astrocytes, the most dominant cells in the central nervous system (CNS), play key roles in maintaining redox homeostasis by providing oxidative stress defense. Hemoglobin (Hb), the primary component released by hemolysis, is an effective activator of astrocytes. Hemin, the product of Hb degradation, is highly toxic due to the induction of reactive oxygen species (ROS). We speculate that Hb-activated astrocytes are resistant to hemin-induced toxicity. To verify our speculation, Hb-pretreated astrocytes were exposed to hemin, intracellular ROS accumulation and cell apoptosis were evaluated. Heme oxygenase 1 (HO-1) and nuclear transcription factor-erythroid 2 related factor (Nrf2) expression were observed to explore the potential mechanism. The results demonstrated that Hb induced upregulation and nuclear translocation of Nrf2 in astrocytes, resulted in HO-1 upregulation, which contributed to reduced ROS accumulation and apoptosis rate . Knocking down Nrf2 expression by siRNA suppressed Hb-induced upregulation of HO-1 expression and increased the susceptibility of Hb-pretreated astrocytes to hemin-induced toxicity. Taken together, Hb-activated astrocytes acquired resistance to hemin-induced toxicity via Nrf2/HO-1 pathway. This phenomenon can be considered as the adaptive self-defense in the pathological process of ICH. Hb pre-warned astrocytes and enhanced their capability of handling the coming hemin “flood”. Nrf2/HO-1 may be employed as a target for neuroprotection after ICH.
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International Journal of Nanomedicine
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2017
·
IF 4.3
Fei Tong,Suhuan Liu,Bing Yan,Xuejun Li,Shiwei Ruan,Shuyu Yang
The purpose of the study was to reveal the therapeutic effect of quercetin (QUE) nanoparticle complex on diabetic nephropathy (DN) by regulating the expression of intercellular adhesion molecular-1 (ICAM-1) on endothelium as compared to free QUE. QUE 10 mg/kg as a single abdominal subcutaneous injection daily for 8 weeks continuously in diabetic rats and 10 mg/kg QUE nanoparticle complex as a single abdominal subcutaneous injection every 5 days, continuously administered for 8 weeks to diabetic rats. Blood and left kidneys were collected; pathological change of kidney, renal function, oxidative stress level, blood glucose level, serum lipid, urine protein, and albumin/creatinine ratio were measured; and neutrophil adhesion, ICAM-1 expression, and CD11b+ cells infiltration were observed. Both QUE and QUE nanoparticle complex preconditioning ameliorated the pathological damage of kidney and improved renal function, alleviated renal oxidative stress injury, restricted inflammatory cells infiltration, and downregulated the ICAM-1 expression as compared to DN group, while QUE nanoparticle complex significantly alleviated this effect.
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高影响因子
Journal of Functional Biomaterials
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2024
·Vol. 15
·期 7
·185
·
IF 5
Yu Wu,Sen Xu,Fengjuan Ding,Weibing Zhang,Haiyan Liu
In this work, a new type of FE-1 COF material is prepared by a reversible imine condensation reaction with diaminoferrocene and diaminodiformaldehyde as materials. The material is connected by imine bonds to form a COF skeleton, and the presence of plenty of nitrogen-containing groups gives the material good hydrophilicity; the presence of metal Fe ions provides the material application potential in the enrichment of phosphopeptides. According to the different binding abilities of N-glycopeptide and phosphopeptide on FE-1 COF, it can simultaneously enrich N-glycopeptide and phosphopeptide through different elution conditions to realize its controllable and selective enrichment. Using the above characteristics, 18 phosphopeptides were detected from α-casein hydrolysate, 8 phosphopeptides were detected from β-casein hydrolysate and 21 glycopeptides were detected from IgG hydrolysate. Finally, the gradual elution strategy was used; 16 phosphopeptides and 19 glycopeptides were detected from the α-casein hydrolysate and IgG hydrolysate. The corresponding glycopeptides and phosphopeptides were identified from the human serum. It proves that the FE-1 COF material has a good enrichment effect on phosphopeptides and glycopeptides.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Ya-Lan Sun,Ya-Hui Sun,Ke Wang,Pengjun Xu,Jun-Feng Dong
Helicoverpa armigera is one of the most serious pests attacking tobacco around the world. Nicotiana rustica is widely used as a trap crop in the fields of Nicotiana tabacum in China. However, the chemical and molecular bases guiding N. rustica recognition in H. armigera are still unknown. In this study, we found 15 volatiles emitted from N. rustica induced EAG responses of H. armigera, while β-ocimene, α-pinene, and linalool were attractive to the moths in Y-tubes. Oviposition tests showed that H. armigera prefers to lay eggs on tobacco plants treated with β-ocimene and α-pinene. Further assays showed that an antenna-enriched chemosensory protein, HarmCSP1, binds robustly with β-ocimene, benzaldehyde, and (Z)-3-hexenyl acetate. Structural analyses revealed that several key amino acid residues are involved in ligand binding. This study increases the understanding of the chemosensory mechanism of H. armigera’s recognition of N. rustica and provides the foundation for the design of attractants for H. armigera management.
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JOURNAL OF CRYSTAL GROWTH
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2012
·
IF 1.726
Shenjie Li,Daocheng Pan
Cu 2 SnSe 3 nanocrystals with a metastable zincblende and wurtzite structure have been successfully synthesized. The crystal structures were confirmed by means of X-ray diffraction and selected area electron diffraction . The lattice mismatch between Cu 2 SnSe 3 and ZnSe is only 0.26%, thus homogeneously alloyed (ZnSe) x (Cu 2 SnSe 3 ) 1− x nanocrystals could be synthesized. The band gaps of nanocrystals can be tuned in a wide range of 2.75 to 0.92 eV; however the bowing parameter as high as 19.1 eV for alloyed (ZnSe) x (Cu 2 SnSe 3 ) 1− x nanocrystals was observed. These low cost and dispersible alloyed (ZnSe) x (Cu 2 SnSe 3 ) 1− x nanocrystals with a targeted band gap have a high potential in thin film solar cells.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
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2012
·
IF 2.237
Yi-Qiang Deng,Shuang-Feng Yin,Chak-Tong Au
Series of silicalite-1 catalysts with particle size varying from 50 to 450 nm were synthesized by hydrothermal method using tetrapropylammonium hydroxide as a template and l-lysine as an additive. The crystal size of silicalite-1 could be easily controlled by regulating the amount of l-lysine. The series of silicalite-1 and their NaOH-modified derivatives were examined as catalysts for vapor-phase Beckmann rearrangement of cyclohexanone oxime to caprolactam in the 330–390 °C range. It was found that the crystal size of silicalite-1 has an effect on catalytic activity, ε-caprolactam selectivity, and catalyst stability. With a decline in crystal size, there was improvement of catalytic activity and catalyst stability. It is deduced that silicalite-1, of a smaller crystal size, is less inclined to pore blocking by coke and hence has better resistance to deactivation.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2012
·
IF 2.237
Rulong Li,Huabin Xing,Qiwei Yang,Xu Zhao,Baogen Su,Zongbi Bao,Yiwen Yang,Qilong Ren
This paper presents an extensive study on the feasibility of ionic liquids (ILs) for the extractive separation of a 1-hexene/n-hexane mixture. Seventeen ILs were tested at 313 K. The extraction selectivities of 1-hexene to n-hexane were in the range 1.5–3.0. The chemical structures of anion and cation in ILs greatly affected the extraction, and the functional groups, such as carbon–carbon double bond, nitrile, ester, and amide, in the ILs led to higher selectivities. The increase of alkyl chain length in cation led to the increase of extraction capacity of 1-hexene. To increase the distribution of 1-hexene in IL phase, silver salt was added to the ILs. It was found that ILs containing silver salt had a high distribution of 1-hexene, as well as high selectivity of 1-hexene against n-hexane. The extraction efficiency increased with the concentration of silver salt in ILs. When the initial feed concentration of 1-hexene was smaller, the selectivity of 1-hexene to n-hexane was higher. A simple mathematical model has been developed to describe the total 1-hexene content in the reaction media under study, on the basis of the formation of complexes between silver and 1-hexene with different stoichiometry. This work proves that the ILs can act as effective extractants in 1-hexene/n-hexane separation.
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