Cell Death Discovery
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2023
·Vol. 9
·期 1
·1-10
·
IF 7
Wang Xianzhe,Shi Wei,Wang Xumei,Lu Jin-Jian,He Ping,Zhang Hongjie,Chen Xiuping
Though palbociclib, a cyclin-dependent kinases 4 and 6 (CDK4/6) inhibitor has been approved for treating breast cancer, two major clinical challenges remain: (i) Triple-negative breast cancer (TNBC) appears to be more resistant to palbociclib, and (ii) Palbociclib-induced senescence-associated secretory phenotype (SASP) has a pro-tumorigenic function. Here we report that combining palbociclib with the STAT3 inhibitor nifuroxazide uncouples SASP production from senescence-associated cell cycle exit. Moreover, we identified nifuroxazide as a CDK2 inhibitor that synergistically promotes palbociclib-induced growth arrest and senescence in TNBC cells. In vitro, the combination of nifuroxazide with palbociclib further inhibited the TNBC cell proliferation and enhanced palbociclib-induced cell cycle arrest and senescence. The modulation of palbociclib-induced SASP by nifuroxazide was associated with the reduction of phosphorylated-STAT3. Nifuroxazide also blocks SASP-dependent cancer cell migration. Furthermore, thermal shift assay and molecular docking of nifuroxazide with STAT3 and CDK2 revealed that it binds to their active sites and acts as a potent dual inhibitor. In vivo, the combination of nifuroxazide with palbociclib suppressed 4T1 tumor growth and lung metastasis. Our data suggest that nifuroxazide enhances the anticancer effects of palbociclib in TNBC by uncoupling SASP production from senescence-associated cell cycle exit and inhibiting CDK2 to promote tumor senescence.
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JOURNAL OF POLYMERS AND THE ENVIRONMENT
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2023
·
IF 5.3
Zhao Yanna,Hou Wentong,Huang Yuqing,Zhang Ting,Zhang Yingying,Wang Yuqi,Bai Xiaowei
In this paper, a bio-based polyamide imide polymer, PDPD (Polymer of D230, Poly hexa methylene diamide and Dialdehyde vanillin), was synthesized via amine aldehyde condensation reaction. PHMD was prepared from diethyl oxalate (ODE) and 1,6-hexanediamine (HAD), and vanillin was dialyzed to give dialdehyde vanillin (DVA). Then polyether amine D230 was introduced to obtain polyamide prepolymer, and triethyl citrate was used as cross-linking agent to prepare bio-based glass polymer material PDPD through bulk polymerization at high temperature. The thermal stability, glass transition temperature, mechanical properties, and energy storage modulus of PDPD material were investigated through thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), dynamic mechanical analysis (DMA), and stress-strain curve. Results show that PDPD possesses excellent thermal stability and high mechanical strength, with a 5% thermal decomposition temperature at 288 °C, a stress of 7 MPa, and a strain of 117%. The storage modulus of PDPD in DMA testing can reach up to 2907 MPa. The anti-creep performance of PDPD is good, and the creep level after two repairs is within 40%. Self-healing testing revealed that PDPD maintained its performance after self-healing. The synthesized polymer is also recyclable, making it an attractive option for environmentally conscious applications.
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RESEARCH ON CHEMICAL INTERMEDIATES
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2023
·
IF 3.3
Guo Zhijin,Liang Xuezheng
The novel porous polymer-based solid acid was synthesized via the polycondensation of 1,4-dichloroxylene (DCX) and following sulfonation using chlorosulfonic acid. The polymer structure could be adjusted by introducing naphthalene. The extremely high surface area of 816 m 2 /g and organic framework provided the mass transfer efficiency and hydrophobic surface. The abundant aromatic rings owned enough sulfonation sites, which resulted in high acidity over 2.7 mmol/g. The novel porous polymer-based solid acid showed high activities for the tetrahydropyranylation of alcohols with the yield over 90% in 5 min at room temperature using 1 wt.% catalyst. The novel solid acid could be recycled for 13 times. The efficient procedure for biodiesel synthesis from waste oil on mild condition was also developed using the solid acid. Both the free fatty acids and glycerides were transformed to the biodiesel products with the yield over 99%. The high surface area, high acidity and the organic framework were the key factors for the high activities, which hold great potential in green chemistry.
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Nature Communications
·
2023
·Vol. 14
·期 1
·1-12
·
IF 16.6
Jiao Jiqing,Yuan Qing,Tan Meijie,Han Xiaoqian,Gao Mingbin,Zhang Chao,Yang Xuan,Shi Zhaolin,Ma Yanbin,Xiao Hai,Zhang Jiangwei,Lu Tongbu
Elucidating the synergistic catalytic mechanism between multiple active centers is of great significance for heterogeneous catalysis; however, finding the corresponding experimental evidence remains challenging owing to the complexity of catalyst structures and interface environment. Here we construct an asymmetric TeN 2 –CuN 3 double-atomic site catalyst, which is analyzed via full-range synchrotron pair distribution function. In electrochemical CO 2 reduction, the catalyst features a synergistic mechanism with the double-atomic site activating two key molecules: operando spectroscopy confirms that the Te center activates CO 2 , and the Cu center helps to dissociate H 2 O. The experimental and theoretical results reveal that the TeN 2 –CuN 3 could cooperatively lower the energy barriers for the rate-determining step, promoting proton transfer kinetics. Therefore, the TeN 2 –CuN 3 displays a broad potential range with high CO selectivity, improved kinetics and good stability. This work presents synthesis and characterization strategies for double-atomic site catalysts, and experimentally unveils the underpinning mechanism of synergistic catalysis.
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Tungsten
·
2023
·
IF 6.6
Fu Yi-Han,Peng Yuan-You,Zhao Lei,He Tian-Qi,Yuan Mei-Mei,Dang Hao,Liu Rui,Ran Fen
Vanadium nitride (VN) has received significant attention as a potential negative electrode material due to its remarkable theoretical and physical properties. However, the electrochemical performance needs to be improved due to its low specific surface area and the issue of being easily dissolved in electrolyte. Researches have shown that the composite structure of carbon-coated nanoscale VN particles could significantly increase the specific surface area of VN, and more importantly, and the carbon matrix can effectively restrain the dissolution of VN particles in the electrolyte, thereby improving its chemical and electrochemical performance, including specific capacity and cycling stability. Herein, VN quantum dots are synthesized and protected by uniformly coating the carbon skeleton on its surface via an in situ strategy of oxidation initiation, which initiates dopamine polymerization, forming a crystalline complex of polydopamine and vanadium. After heat treatment, the product is a composite material with VN quantum dots embedded in the amorphous carbon skeleton. The resulting small-sized and crosslinked carbon particles formed a graded pore structure, more reasonable channels provided for electrolyte ions transport quickly. When the composite material was used as the negative electrode material for supercapacitors, it exhibits the specific capacity of 391.9 F·g −1 at a current density of 0.5 A·g −1 and excellent capacity retention rate of 83.6% after 5000 cycles. The synthesis method of this quantum dot could be applied to other metal compounds and has potential applications in catalysis, environmental protection, and other fields.
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npj Biofilms and Microbiomes
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2023
·Vol. 9
·期 1
·1-14
·
IF 9.2
Hu Jie,Wu Yifan,Kang Luyuan,Liu Yisi,Ye Hao,Wang Ran,Zhao Jinbiao,Zhang Guolong,Li Xilong,Wang Junjun,Han Dandan
Elimination of specific enteropathogenic microorganisms is critical to gut health. However, the complexity of the gut community makes it challenging to target specific bacterial organisms. Accumulating evidence suggests that various foods can change the abundance of intestinal bacteria by modulating prophage induction. By using pathogenic Escherichia coli ( E. coli ) ATCC 25922 as a model in this research, we explored the potential of dietary modulation of prophage induction and subsequent bacterial survival. Among a panel of sugars tested in vitro, D -xylose was shown to efficiently induce prophages in E. coli ATCC 25922, which depends, in part, upon the production of D -lactic acid. In an enteric mouse model, prophage induction was found to be further enhanced in response to propionic acid. Dietary D -xylose increased the proportion of Clostridia which converted D -lactic acid to propionic acid. Intestinal propionic acid levels were diminished, following either oral gavage with the dehydrogenase gene ( ldhA )-deficient E. coli ATCC 25922 or depletion of intestinal Clostridia by administration of streptomycin. D -Xylose metabolism and exposure to propionic acid triggered E. coli ATCC 25922 SOS response that promoted prophage induction. E. coli ATCC 25922 mutant of RecA, a key component of SOS system, exhibited decreased phage production. These findings suggest the potential of using dietary components that can induce prophages as antimicrobial alternatives for disease control and prevention by targeted elimination of harmful gut bacteria.
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BIOLOGIA
·
2023
·
IF 1.5
Guo Yu,Guo Xian-Guo,Song Wen-Yu,Lv Yan,Yin Peng-Wu,Jin Dao-Chao
Chiggers are a group of ectoparasites and they are the vector of scrub typhus. Although the Ryukyu mouse ( Mus caroli ) is a common species of rodent in some outdoor habitats, few studies are about the mouse itself and its ectoparasites. To date, there has been no specific report on chiggers of M. caroli . Based on the field investigation in southwest China from 2001 to 2019, this paper reported the infestation and distribution of chiggers on M. caroli for the first time. Among the 91 sites investigated, 263 Ryukyu mice were captured at 18 sites, and 678 chiggers were collected from the mice. The collected chiggers were identified to be one family, two subfamilies, six genera, and 16 species with relatively low species diversity. The overall infestation prevalence ( P M ), mean abundance ( MA ), and mean intensity ( MI ) were P M = 27.38%, MA = 2.58 chiggers/per mouse, and MI = 9.42 chiggers/per mouse respectively. The dominant chigger species are Walchia ewingi and W. micropelta with a total constituent ratio of C r = 94.10% (638/678), and they were of aggregated distribution among different individuals of the host M. caroli . The distribution of two dominant chigger species seemed to be mutually independent with the association coefficient V ≈ 0 ( p < 0.001). The infestation ( P M , MA , and MI ) of M. caroli with chiggers greatly fluctuated in different geographical landscapes, altitudes, and latitudes with an obvious environmental heterogeneity. The male and adult M. caroli harbored much more chigger mites with significantly higher infestations ( P M , MA and MI ) than the female and juvenile mice. The theoretical curve of species abundance distribution of the chigger mite community on M. caroli was fitted by Preston lognormal distribution model with fitting goodness R 2 = 0.77. Based on the iChao 1 formula, the expected total number of chigger species on the mice was estimated to be 24 species.
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IONICS
·
2023
·
IF 2.8
Wei Yunping,Wang Nan,Liu Xingcen,Lin Meng
In this experiment, a copper-based metal–organic framework (Cu-BTC) was successfully prepared and utilized to compose a copper-tannic acid (TA) complex by in situ tannic acid etching on indium tin oxide (ITO) glass. The Cu-BTC was obtained by the electrodeposition method with a voltage of − 1.6 V. The TA-Cu complex retains the frame structure of the metal–organic framework and has good electrochemical properties. Cyclic voltammetry (CV) and amperometric methods were used to estimate the performance of the TA-Cu-modified electrode in glucose detection. The current response of the TA-Cu sensor shows a linear relationship with glucose concentration at 0.5 V in NaOH solution. The modified electrode as an electrochemical glucose sensor exhibits good sensitivity with a low detection limit of 0.002 mM. The TA-Cu-modified electrode also exhibited good stability, anti-interference, and repeatability in glucose detection.
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Journal of Infrared Millimeter and Terahertz Waves
·
2023
·Vol. 44
·期 9
·780-794
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IF 2.9
Guan Hanxiao,Xie Chen,Cao Yuqi,Huang Pingjie,Hou Dibo,Zhang Guangxin
In this paper, we propose a discrimination model for abnormal sialic acid content using terahertz time and frequency-domain features of samples in an attempt to explore the significance of human serum sialic acid content in early cancer screening. Specifically, the time-domain spectral features were obtained by applying variational mode decomposition (VMD) for time-domain spectrum preprocessing and composite multi-scale entropy (CMSE) for feature extraction. The frequency-domain spectral features were acquired using principal component analysis (PCA). The model was then constructed on the basis of support vector machine (SVM). To verify the reliability of this discrimination model, we used three different concentrations of albumin solutions to simulate the serum environment in the human body and mixed them with sialic acid solutions of eight different concentrations. The results indicate that our discrimination model achieved an accuracy of 91.01% for the detection of abnormal sialic acid content. This study provides a foundation for the accurate quantitative detection of biomarkers in human serum.
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Scientific Reports
·
2023
·Vol. 13
·期 1
·1-14
·
IF 4.6
Alishiri Mahsa,Gonbadi Maryam,Narimani Mehdi,Abdollahi Seyyed Amirreza,Shahsavaripour Negin
The contamination of the aquatic environment with antibiotics is among the major and developing problems worldwide. The present study investigates the potential of adsorbent magnetite-chitosan nanoparticles (Fe 3 O 4 /CS NPs) for removing trimethoprim (TMP) and sulfamethoxazole (SMX). For this purpose, Fe 3 O 4 /CS NPs were synthesized by the co-precipitation method, and the adsorbent characteristics were investigated using XRD, SEM, TEM, pH zpc , FTIR, and VSM. The effect of independent variables (pH, sonication time, adsorbent amount, and analyte concentration) on removal performance was modeled and evaluated by Box–Behnken design (BBD). The SEM image of the Fe 3 O 4 /CS adsorbent showed that the adsorbent had a rough and irregular surface. The size of Fe 3 O 4 /CS crystals was about 70 nm. XRD analysis confirmed the purity and absence of impurities in the adsorbent. TEM image analysis showed that the adsorbent had a porous structure, and the particle size was in the range of nanometers. In VSM, the saturation magnetization of Fe 3 O 4 /CS adsorbent was 25 emu g −1 and the magnet could easily separate the adsorbent from the solution. The results revealed that the optimum condition was achieved at a concentration of 22 mg L −1 , a sonication time of 15 min, an adsorbent amount of 0.13 g/100 mL, and a pH of 6. Among different solvents (i.e., ethanol, acetone, nitric acid, and acetonitrile), significant desorption of TMP and SMX was achieved using ethanol. Also, results confirmed that Fe 3 O 4 /CS NPs can be used for up to six adsorption/desorption cycles. In addition, applying the Fe 3 O 4 /CS NPs on real water samples revealed that Fe 3 O 4 /CS NPs could remove TMP and SMX in the 91.23–95.95% range with RSD (n = 3) < 4. Overall, the Fe 3 O 4 /CS NPs exhibit great potential for removing TMP and SMX antibiotics from real water samples.
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CELLULOSE
·
2023
·Vol. 30
·期 16
·10285-10296
·
IF 5.7
Gao Yan,Zhang Yuangong,Wang Yu,Liu Bing,Liu Feng,Ba Xinwu
In this paper, a cellulose-based hydrogel with the ability to lower glucose levels, scavenge reactive oxygen species (ROS), and colorimetrically monitor early lesions was synthesized to address the issue of glucose and H 2 O 2 overexpression on the wound surface. Briefly, boronic acid pinacol ester containing phenolphthalein (BAPE-PPH) was employed as a crosslinker to react with hydroxypropyl methylcellulose (HPMC) to afford hydrogels (HPMC@PPH-Hs) with good biocompatibility. Because glucose has higher affinity for the phenylboronic ester group than cellulose derivatives, the HPMC@PPH-Hs showed excellent glucose-lowering performance. The presence of B-C bonds in the network endows the as-obtained hydrogel with H 2 O 2 sensitivity, and cleavage of the B-C bonds forces the transformation of BAPE-PPH moieties into PPH, giving rise to colorimetric behaviour. Thus, the obtained HPMC@PPH-Hs provide information to enrich the family of smart hydrogels, and its potential application in diabetic wound healing may be desirable. Graphical abstract
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MICROCHIMICA ACTA
·
2023
·Vol. 190
·期 10
·1-10
·
IF 5.7
Wu Yongju,Yi Jinfei,Su Aiwen,Zhang Yanli,Wang Hongbin,Yang Lijuan,Yang Wenrong,Pang Pengfei
T4 polynucleotide kinase (T4 PNK) helps with DNA recombination and repair. In this work, a phosphate pillar[5]arene@palladium nanoparticles@reduced graphene oxide nanocomposite (PP5@PdNPs@rGO)–based electrochemical biosensor was created to identify T4 PNK activities. The PP5 used to complex toluidine blue (TB) guest molecules is water-soluble. With T4 PNK and ATP, the substrate DNA, which included a 5ʹ-hydroxyl group, initially self-assembled over the gold electrode surface by chemical adsorption of the thiol units. Strong phosphate-Zr 4+ -phosphate chemistry allowed Zr 4+ to act as a bridge between phosphorylated DNA and PP5@PdNPs@rGO. Through a supramolecular host–guest recognition connection, TB molecules were able to penetrate the PP5 cavity, where they produced a stronger electrochemical response. With a 5 × 10 −7 U mL −1 detection limit, the electrochemical signal is linear in the 10 −6 to 1 U mL −1 T4 PNK concentration range. It was also effective in measuring HeLa cell lysate–related PNK activities and screening PNK inhibitors. Nucleotide kinase–target drug development, clinical diagnostics, and screening for inhibitors all stand to benefit greatly from the suggested technology, which offers a unique sensing mechanism for kinase activity measurement. Graphical Abstract
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危险品化学品经营许可证（带存储）