Materials
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2016
·Vol. 9
·期 11
·913
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IF 2.728
Guihua Cui,Zhengguo Gao,Nannan Qiu,Toshifumi Satoh,Toyoji Kakuchi,Qian Duan
Regioselective modification ofd-glucosamine (2-amino-2-deoxy-d-glucopyranose, GA) through C-1 and C-2 positions to synthesized thermo-responsive D-Glucosamine-poly(N-iso-propylacrylamide) (PNIPAM) via atom transfer radical polymerization (ATRP) was investigated for the first time. Two different schemes of the synthesis for GA derivatives (GA-PNIPAM (i) and (ii)) with well-defined structures using 3,4,6-tri-o-acetyl-2-deoxy-2-phthalimido-β-d-glucopyranose and 1,3,4,6-tetra-o-acetyl-2-amino-2-deoxy-β-d-glucopyranose intermediates were examined. The GA-PNIPAM (ii) had an amino at C-2 position, while there was a hydroxyl in GA-PNIPAM (i) at this position. Both the resulting oligomers (i) and (ii) had a narrow dispersity, and no significant cytotoxic response of copolymers (i) and (ii) was observed in the cell line over the concentration range from 0.1 μg/mL to 1000 μg/mL at any of the exposure times. In addition, it was discovered that GA-PNIPAM (i) and (ii) inhibited the proliferation of Human Hepatocellular Carcinoma Cells HepG2 as the concentration and the time changed, and the inhibitory activity of polymer (ii) was higher than that of he (i). The results suggest that the GA-PNIPAM polymers show excellent biocompatibility in vitro.Keywords:poly(N-isopropylacrylamide) (PNIPAM);d-glucosamine (GA);atom transfer radical polymerization (ATRP);lower critical solution temperature (LCST)
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CHEMICO-BIOLOGICAL INTERACTIONS
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2024
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IF 4.7
Aijie Zhang,Shiqi Dong,Fanlong Yang,Yufeng Zhang,Yunhua Teng,Weisheng Tang,Jianfeng Liu,Huirong Fan,Ying Peng,Jiang Zheng
ABSTRACT Pharmacokinetic changes induced by radiation following radiotherapy (“RT-PK” phenomenon) are of great significance to the effectiveness and safety of chemotherapeutic agents in clinical settings. The aims of this study were to clarify the organic anion transporters (Oats) involved in the “RT-PK” phenomenon of bestatin in rats following X-ray irradiation and to elucidate its potential mechanism via vitamin D signalling. Pharmacokinetic studies, uptake assays using rat kidney slices and primary proximal tubule cells, and molecular biological studies were performed. Significantly increased plasma concentrations and systemic exposure to bestatin were observed at 24 and 48 h following abdominal X-ray irradiation, regardless of oral or intravenous administration of the drugs in rats. Reduced renal clearance and cumulative urinary excretion of bestatin were observed at 24 and 48 h post-irradiation in rats following intravenous administration. The uptake of the probe substrates p -aminohippuric acid and oestrone 3-sulphate sodium in vitro and the expression of Oat1 and Oat3 in vivo were reduced in the corresponding models following irradiation. Moreover, the upregulation of the vitamin D receptor (Vdr) in mRNA and protein levels negatively correlated with the expressions and functions of Oat1 and Oat3 following irradiation. Additionally, elevated plasma urea nitrogen levels and histopathological changes were observed in rats after exposure to irradiation. The “RT-PK” phenomenon of bestatin occurs in rats after exposure to irradiation, possibly resulting in the regulation of the expressions and activities of renal Oats via activation of the Vdr signalling pathway.
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BIOORGANIC CHEMISTRY
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2024
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IF 4.5
Muhammad Waheed Iqbal,Xinrui Tang,Tahreem Riaz,Shahid Mahmood,Yufei Zhang,Mei Zhao,Junhua Yun,Jia Li,Xianghui Qi
6-Deoxy- l -sorbose (6-DLS) is an imperative rare sugar employed in food, agriculture, pharmaceutical and cosmetic industeries. However, it is a synthetic and very expensive rare sugars, previously synthesized by chemo-enzymatic methods through a long chain of chemical processes. Recently, enzymatic synthesis of rare sugars has attracted a lot of attention due to its advantages over synthetic methods. In this work, a promising approach for the synthesis of 6-DLS from an inexpensive sugar l -fucose was identified. The genes for l -fucose isomerase from Paenibacillus rhizosphaerae (Pr-LFI) and genes for d -tagatose-3-epimerase from Caballeronia fortuita (Cf-DTE) have been used for cloning and co-expression in Escherichia coli , developed a recombinant plasmid harboring pANY1-Pr-LFI/Cf-DTE vector. The recombinant co-expression system exhibited an optimum activity at 50 °C of temperature and pH 6.5 in the presence of Co 2+ metal ion which inflated the catalytic activity by 6.8 folds as compared to control group with no metal ions. The recombinant co-expressed system was stable up to more than 50 % relative activity after 12 h and revealed a melting temperature (T m ) of 63.38 °C exhibiting half-life of 13.17 h at 50 °C. The co-expression system exhibited, 4.93, 11.41 and 16.21 g/L of 6-DLS production from initial l -fucose concentration of 30, 70 and 100 g/L, which equates to conversion yield of 16.44 %, 16.30 % and 16.21 % respectively. Generally, this study offers a promising strategy for the biological production of 6-DLS from an inexpensive substrate l -fucose in slightly acidic conditions with the aid of co-expression system harboring Pr-LFI and CF-DTE genes.
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JOURNAL OF ETHNOPHARMACOLOGY
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2024
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IF 4.8
Yun-Lu Xu,Xue-Jian Li,Wei Cai,Wen-Ying Yu,Jing Chen,Qin Lee,Yong-Jun Choi,Fang Wu,Ying-Jun Lou,Hua-Zhong Ying,Chen-Huan Yu,Qiao-Feng Wu
Ethnopharmacological relevance Viral pneumonia is the leading cause of death after SARS-CoV-2 infection. Despite effective at early stage, long-term treatment with glucocorticoids can lead to a variety of adverse effects and limited benefits. The Chinese traditional herb Pogostemonis Herba is the aerial part of Pogostemon Cablin (Blanco) Benth., which has potent antiviral, antibacterial, anti-inflammatory, and anticancer effects. It was used widely for treating various throat and respiratory diseases, including COVID-19, viral infection, cough, allergic asthma, acute lung injury and lung cancer. Aim of the study To investigate the antiviral and anti-inflammatory effects of chemical compounds from Pogostemonis Herba in SARS-CoV-2-infected hACE2-overexpressing mouse macrophage RAW264.7 cells and hACE2 transgenic mice. Materials and methods The hACE2-overexpressing RAW264.7 cells were exposed with SARS-CoV-2. The cell viability was detected by CCK8 assay and cell apoptotic rate was by flow cytometric assay. The expressions of macrophage M1 phenotype markers (TNF-α and IL-6) and M2 markers (IL-10 and Arg-1) as well as the viral loads were detected by qPCR. The mice were inoculated intranasally with SARS-CoV-2 omicron variant to induce viral pneumonia. The levels of macrophages, neutrophils, and T cells in the lung tissues of infected mice were analyzed by full spectrum flow cytometry. The expressions of key proteins were detected by western blot assay. Results Diosmetin-7-O-β-D-glucopyranoside (DG) presented the strongest anti-SARS-CoV-2 activity. Intervention with DG at the concentrations of 1.25-50 μM not only reduced the viral replication, cell apoptosis, and the productions of inflammatory cytokines (IL-6 and TNF-α) in SARS-CoV-2-infected RAW264.7 cells, but also reversed macrophage polarity from M1 to M2 phenotype. Furthermore, treatment with DG (25-100 mg/kg) alleviated acute lung injury, and reduced macrophage infiltration in SARS-COV-2-infected mice. Mechanistically, DG inhibited SARS-COV-2 gene expression and HK3 translation via targeting YTHDF1, resulting in the inactivation of glycolysis-mediated NF-κB pathway. Conclusions DG exerted the potent antiviral and anti-inflammatory activities. It reduced pneumonia in SARS-COV-2-infected mice via inhibiting the viral replication and accelerating M2 macrophage polarization via targeting YTHDF1, indicating its potential for COVID-19 treatment.
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Food Bioscience
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2024
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IF 4.8
Linhui Feng,Heping Cui,Khizar Hayat,Xiaoming Zhang,Chi-Tang Ho
The Amadori rearrangement product (GR-ARP) derived from glutathione/ribose mixture (GSH/Rib) was prepared through spray drying characterized by short-term high temperature dehydration. The water activity of spray-dried samples containing GR-ARP decreased continuously with an intensified degree of dehydration. Lower water activity (from 0.23 to 0.13) and higher GR-ARP yield (from 46.56% to 64.02%) in the spray-dried products were found with the increase of inlet temperature, the slowdown of feed speed, and the alkalization of stock solution. Moreover, during the spray drying and formation of powders, the intensified dissipation of bound water to free water promoted intermolecular condensation that compensated bound water, favoring the directed conversion of GSH/Rib into GR-ARP. Compared to rotary evaporation at 80 °C, spray drying enabled a stronger restraint of amide cleavage and sulfhydryl oxidation in GSH, which reduced the yield of GSH-derived byproducts by 16.39%. Thus, GR-ARP was achieved a peak yield of 64.02% at an initial pH of 7.00 based on more efficient intermolecular condensation and controlled side reactions of GSH when the inlet temperature was set at 170 °C. The spray-dried sample containing richer GR-ARP and other flavor precursors showed a more similar flavor profile to the heated GSH/Rib mixture (complete Maillard reaction products) than other GR-ARP-related thermal processing samples, such as vacuum-dehydrated, GR-ARP, GR-ARP + GSH, and GR-ARP + Rib samples, revealing a great potential of the ARP mixture prepared by spray drying for flavor enhancement.
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ADVANCED FUNCTIONAL MATERIALS
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2025
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IF 19
Fan Li,Shang Sun,Lina Ma,Wenjing Zhang,Min Zhou,Yu Fu,Hengrui Guo,Xueying Su,Jinliang Shi,Hao Luo,Yang Yang
The intrinsically low electrical conductivity of elemental iodine and its slow redox kinetics represent two significant obstacles that hinder the commercialization of aqueous zinc-iodine batteries. These challenges can be addressed by modulating the adsorption-desorption equilibrium of iodide ions in the active material. This study presented a synergistic strategy of “Electronic Modulation” and “Phase Transition Engineering” to optimize the catalytic adsorption-desorption dynamics. A dual-designed Fe ions doped VO 2- x heterostructure anchored on reduced graphene oxide (Fe-VO 2- x /rGO) is demonstrated to synchronously adjust iodine adsorption and boost redox kinetics. The Fe-induced electronic structure modulation elevated the d-band center of vanadium, creating catalytic sites to promote the desorption of iodide ions and facilitate their diffusion. Concurrently, the phase transition from VO 2- x to V 2 O 5- n within the heterostructure establishes a 3D conductive network, synergizing with rGO to ensure rapid ion/electron transport. This “adsorption-catalysis-transport” collaborative mechanism enabled the Fe-VO 2- x /rGO cathode to achieve an ultrahigh capacity retention of 81.48% after 60,000 cycles at 20 A g −1 , surpassing state-of-the-art zinc-iodine systems. The work provides a paradigm for manipulating interfacial dynamics through electronic and crystallographic dual regulation in conversion-type batteries.
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TALANTA
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2025
·
IF 6.1
Liang Liu,Shiao Ren,Yangyi Hao,Lijuan Yue,Chenli Yue,Longyu Li,Wenlei Zhang,Zengliang Gao,Xin Hai
The aim of this study was to establish a simple, sensitive, and robust ultra-high performance liquid chromatography coupled with fluorescence detection method (UPLC-FLD) for the determination of chiral D/L-serine in cells. D/L-serine in cells were derivatizated by o-phthalaldehyde (OPA) and N-acetyl-L-cysteine (NAC). Carbocisteine was selected as the internal standard. The derivatives were separated on a C18 column by gradient elution. The excitation and emission wavelengths for fluorescence determination are 340 nm and 450 nm, respectively. The retention time of D-serine and L-serine was 23.3 and 23.9 min respectively, which presented a perfect separation. The accuracy of D-serine and L-serine were ranged from 96.46 % to 109.63 % and 95.50 %–102.20 %, respectively. The precision of D-serine and L-serine were ranged from 4.34 % to 14.56 % and 3.56 %–13.73 %. The limit of quantitation of D-serine and L-serine were 0.1 nmol/mL. The concentration of D/L-serine varies in different cell lines. This method can satisfy the determination of D/L-serine in astrocytes and other cells, which can be used for the study of D/L-serine metabolism and related mechanisms. In short, we have established a simple, stable, reliable and robust method for the determination of D/L-serine in cells. In addition, the D/L-serine levels in different cells were quantified in this study, which can provide a reference for the study of D/L-serine metabolism in cells.
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JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
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2025
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IF 6.2
Yuvaraj Ravikumar,Abdullah Arsalan,Xinrui Tang,Guoyan Zhang,Xianghui Qi
l-Arabinose isomerase (l-AI) catalyzes d-galactose to produce the rare, industrially important sugar d-tagatose. Enzyme stability is vital for its application in industrial processes, and rational design-based protein engineering methods have been employed to improve its stability. This study employed molecular dynamics simulations (MDS)-guided rational redesign of flexible regions to improve the thermostability of mesophilic Bifidobacterium adolescentis l-AI (Ba-l-AI). Comparative MDS (200 ns, 340–360 K) of thermophilic l-AIs (Geobacillus kaustophilus Gk-l-AI and Thermotoga maritima Tm-l-AI) and mesophilic Ba-l-AI identified flexible regions in Ba-l-AI via RMSF and ionic interaction. Five stabilizing mutations (G78C, K46R, N187R, K112R, and N190R) were introduced to provide rigidness. MDS of the mutant revealed reduced RMSF/RMSD, improved compactness, and enhanced conformational stability across temperatures, supported by free-energy landscape analysis resembling thermophilic profiles. Experimentally, the mutant exhibited superior thermal stability (72 °C), broader pH/temperature tolerance, and efficient d-tagatose production. The redesigned Ba-l-AI’s rigid backbone and stability highlight its potential for industrial d-tagatose biosynthesis.
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Nano Energy
·
2025
·
IF 17.1
Meimei Zhu,Shuhan Cao,Shunyu Wang,Xingchen Liu,Xiangwen Wu,Dongwen Zou,Hongyang Li,Yaowu He,Hong Meng
Donor-Acceptor (D-A) copolymers are critical for cost-effective and energy-efficient electrochromic devices (ECDs) and smart windows. In this study, a type of copolymers with dual-introduction of alkyl thiophene as π spacer and fluorine are designed for highly efficient ECDs, contributing to superior color efficiency (CE). Through π spacer modification, the twist angle as well as the optical bandgap of the copolymers (PDT-Th-BT) are minimized via blocking the F…O noncovalent repulsive interaction. ECDs based on PDT-Th-BT1F are achieved with high CE of 1440 cm²/C, along with electrochromic contrast up to 41.6%. This CE is the best yet among ECDs based on fluorination D-A type copolymers according to literature. Furthermore, smart window made from PDT-Th-BT1F contribute a 5 ℃ cooling down compared to the glass in the building models and an energy saving of 631,257.14 kWh in a 26,404.54 m 2 building model, greatly conducive to environmental protection. This work presents a viable strategy for the development of highly efficient ECDs and smart windows.
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SEPARATION AND PURIFICATION TECHNOLOGY
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2024
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IF 8.1
Fei Fan,Shiyu Han,Ying Li,Lu Qi,Jie Kang,Zhiping Chen,Chang Tian,DeChao Wang,Wenwu Zhou
The microenvironments including the surface hydroxyls , the oxygen vacancies , and the d-band center of hierarchical TS-1 zeolite pickled by hydrochloric acid were regulated by ZrO 2 , the effect of ZrO 2 on the physicochemical properties and the catalytic performances of the corresponding NiMoS supported catalysts were studied. The experimental and theoretical calculation results show that the d-band center, the surface hydroxyl groups , and the oxygen vacancies were regulated after incorporation of ZrO 2 . Resulting in the modulated acidity of the support and the metal support interaction of the catalysts, further promoting the formation of type-II NiMoS active phases. The catalytic performance evaluations show that catalyst NiMo/1-ZAT exhibits the superior HDS performance, kinetic study further demonstrated that the enhanced catalytic activity was mainly contributed by the improved direct desulfurization activity generated from the moderate support acidity, suitable MSI, highest sulfidation degrees, most type-II NiMoS active phase and highest activity of individual active sites.
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International Journal of Nanomedicine
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2023
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IF 8
Hongzhi Qiao,Lihua Chen,Tianqi Rui,Jingxian Wang,Ting Chen,Tingming Fu,Junsong Li,Liuqing Di
Andrographolide (ADG) is a diterpenoid isolated from Andrographis paniculata with a wide spectrum of biological activities, including anti-inflammatory, anticancer and hepatoprotective effects. However, its poor water solubility and efflux by P-glycoprotein have resulted in lower bioavailability. In this study, ADG nanosuspensions (ADG-NS) were prepared using a wet media milling technique followed by freeze drying. d-α-Tocopheryl polyethylene glycol 1000 succinate (TPGS), a surfactant that inhibits P-glycoprotein function, and sodium lauryl sulfate were used as surface stabilizers. A Box–Behnken design was used to optimize the nanosuspension preparation. The products of these optimal preparation conditions were amorphous and possessed much faster dissolution in vitro than a coarse powder of ADG. The particle size and redispersibility index of the freeze-dried ADG-NS were 244.6±3.0 nm and 113%±1.14% (n=3), respectively. A short-term stability study indicated that the freeze-dried ADG-NS could remain highly stable as nanosuspensions during the testing period. A test of transport across a Caco-2 cell monolayer revealed that the membrane permeability (Papp) of ADG-NS was significantly higher than the permeability of the ADG coarse powder or ADG-NS without TPGS (P<0.01). Compared to the ADG coarse powder, a physical mixture, commercial dripping pills and ADG-NS without TPGS, ADG-NS exhibited significantly higher plasma exposure with significant enhancements in Cmax and area under the curve of plasma concentration versus time from zero to the last sampling time (AUC0−t) (P<0.01). An evaluation of the anti-inflammatory effect on Carr-induced paw edema demonstrated that the ADG-NS were more effective in reducing the rate of paw swelling, producing a greater increase in the serum levels of nitric oxide (NO), Interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) (P<0.01) and an increase in superoxide dismutase activity (P<0.05) compared to the ADG coarse powder. This study indicated that nanosuspensions could act as an effective delivery device for ADG to enhance its oral bioavailability and biological efficacy.
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JOURNAL OF ETHNOPHARMACOLOGY
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2023
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IF 5.4
Ao Xue,Deping Zhao,Chenyu Zhao,Xue Li,Meng Yang,Hongmei Zhao,Can Zhao,Xia Lei,Jianli Wu,Ning Zhang
Ethnopharmacological relevance Zhimu-Huangbo (ZB) herb pair is a common prescription drug used by physicians of all dynasties, and has significant neuroprotective effect, such as the ZB can significantly promote neuronal cell regeneration, repair neuronal damage, and improve cognitive disorders. However, its ingredients are urgently needed to be identified and mechanisms is remained unclear. Aim of the study Using ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS), the study of neuroprotective mechanism of Zhimu-Huangbo extract (ZBE) is investigated, and the network pharmacology technology and experimental validation is also performed. Material and methods Firstly, UPLC-Q-TOF-MS technology was used to characterize the chemical components contained in the ZBE. After that, the TCMSP database and the Swiss Target Prediction method were used to search for potential target genes for ZBE compounds. At the same time, the OMIM and GeneCards disease databases were used to search for Alzheimer's disease (AD) targets and expanded with the GEO database. Then, GO and KEGG enrichment analysis was performed using OECloud tools. Subsequently, the potential mechanism of ZBE therapeutic AD predicted by network pharmacological analysis was experimentally studied and verified in vitro . Results In the UPLC-Q-TOF-MS analysis of the ZBE, a total of 39 compounds were characterized including Neomangiferin, Oxyberberine, Timosaponin D, Berberine, Timosaponin A-III, Anemarsaponin E, Timosaponin A-I, Smilagenin and so on. A total of 831 potential targets and 13995 AD-related target genes were screened. A further analysis revealed the number of common targets between ZBE and AD is 698. Through GO and KEGG enrichment analysis, we found that ZBE's anti-AD targets were significantly enriched in autophagy and mitochondrial autophagy related pathways. The results of cell experiments also confirmed that ZBE can promote mitochondrial autophagy induced by D-galactose (D-gal) HT22 cells through the PTEN-induced kinase 1/Parkin (PINK1/Parkin) pathway. Conclusion ZBE can promote autophagy of mitochondria and play a protective role on damaged neurons.
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