JOURNAL OF COLLOID AND INTERFACE SCIENCE
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2024
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IF 9.4
Luping Feng,Jianghong Wang,Lixiang Zhang,Jiadong Li,Yifan Zhang,Minghong Xu,Peisong Tang,Hua Wang
Tubular Co 9 S 8 /CdS heterostructures have been successfully synthesized by in-situ growing CdS onto Co 9 S 8 nanotubes through a simultaneous immobilization and in-situ reduction strategy. It turned out that the so-obtained heterostructure with Co 9 S 8 /CdS molar ratio of 1/10 can display a broad light absorption edge and especially much enhanced capacity for photocatalytic reduction of Cr(VI) under visible light . The characterization analysis and experimental results suggested that an interfacial electrostatic field between Co 9 S 8 and CdS elements in the heterostructure could be constructed due to their different Fermi levels , allowing for more quantities of highly reductive electrons to participate in the photocatalytic reaction. Therefore, the so-obtained Co 9 S 8 /CdS (1/10) heterostructures could achieve the photocatalytic reduction efficiency of 100% within 20 min, which was more than two and four times larger than that of pristine CdS and Co 9 S 8 , respectively. Moreover, the possible photocatalytic reaction mechanism for reducing Cr(VI) was investigated and found to follow the direct Z-scheme charge transfer pathway. This novel fabrication route for composite photocatalysts with tubular heterostructures could lead to the widespread implementations for the elimination of various harmful pollutants in the process of environmental governance.
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JOURNAL OF ELECTROANALYTICAL CHEMISTRY
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2014
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IF 2.871
Kan Zhan,Hailing Liu,Hui Zhang,Yulu Chen,Henmei Ni,Ming Wu,Dongmei Sun,Yu Chen
Poly(methacrylic acid-co-acrylamide) (P(MAA-co-AAM)), noncovalently attached on the surface of multiwalled carbon nanotubes (MWCNTs) through hydrophobic interactions, hydrogen bonding or electrostatic attraction, was employed for the first time as a novel matrix for enzyme immobilization to develop highly sensitive amperometric biosensors. The effective interaction between P(MAA-co-AAM) and MWCNTs, and the structural morphology of the as-prepared P(MAA-co-AAM)–MWCNTs nanocomposite were characterized by Fourier transform infrared (FT-IR) spectroscopy, X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Further experimental results show that the solubility and stability of as-prepared P(MAA-co-AAM)–MWCNTs nanocomposites in water are better than that of pristine MWCNTs, and no significant electronic and structural change was observed after functionalization. When myoglobin (Mb) was selected as a model protein, studies by Ultraviolet–visible (UV–vis) and Circular dichroism (CD) spectroscopy indicate that the encapsulated Mb in the as-prepared P(MAA-co-AAM)–MWCNTs nanocomposites, was kept in a near-native state, indicating the P(MAA-co-AAM)–MWCNTs nanocomposites have good biocompatibility for protein/enzyme immobilization. The fabricated electrochemical biosensor based on the immobilized Mb reveals fast response of less than 3 s, wide linear range from 1.47 × 10 −6 M to 4.76 × 10 −3 M and good detection limit as low as 7.60 × 10 −7 M toward the electro-determination of hydrogen peroxide (H 2 O 2 ) under optimal experimental conditions.
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CHEMICAL ENGINEERING JOURNAL
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2020
·
IF 10.652
Yibo Zhang,Peng Hu,Yaseen Muhammad,Ying Tang,Shan Shao,Zhu Gao,Jiaxing Wang,Ruimeng Wang,Yang Hu,Lihan Kuang,Zhongxing Zhao,Zhenxia Zhao
Hollow nano-sphere NH 2 -MIL88(Fe) (HS NMIL88(Fe)) with interfacial defects was fabricated to load laccase as a host. Through metal multi-coordination, dopamine engineered large amount of defective pores with large size on NH 2 -MIL88(Fe) interface. This defect-interfacial structure provided a high-density host for laccases, which efficiently protected enzyme from structure deformation and then inhibited inactivation. Besides, the unique hollow structure will help to fasten reactant diffusivity, and thus improved the catalytic reaction process. Experimental results showed that the maximum immobilized capacity and maximum activity recovery for laccase reached up to 213.2 mg/g and 75.8% beyond many state-of-the-art enzyme hosts, respectively. Meanwhile, the immobilized laccase exhibited better pH, thermal and storage stabilities on HS NMIL88(Fe) than free and loaded on NMIL88(Fe) ones. Thermal deactivation kinetics analysis showed that HS NMIL88(Fe)-Lac had much higher apparent activation energies of (105.2 kJ/mol) than that of free laccase (89.2 kJ/mol). Catalyzing decolorization performance of dye Remazol Brilliant Blue R (RBBR) was finally carried out and exhibited the decolorization rate of 1.8 times and 2.6 times faster than solid NMIL88(Fe)-Lac and free laccase, respectively. Moreover, the interaction mechanism of laccase for RBBR was investigated and revealed by molecular docking.
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BIORESOURCE TECHNOLOGY
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2025
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IF 9
Meishan Guo,Hongli Chao,Yang Sun,Dongyu Gu,Jing Wang,Yi Wang,Dajun He,Dezhi Huang,Jing Tian,Yi Yang
Enzymatic epoxidation offers eco-friendly advantages over chemical methods. This work developed a modular dual-enzyme reactor through artificial antibody-antigen directed immobilization for in-situ epoxide biosynthesis. Magnetic artificial antibodies were synthesized using hydroquinone and 4-aminopyrimidine templates, while 2,5-dihydroxybenzaldehyde and 4-aminopyrimidine-5-carbaldehyde were employed to modify glucose oxidase (GOx) and lipase (LIP), respectively, forming artificial antigens. The dual-enzyme reactor was constructed through affinity recognition between artificial antibodies and antigens, a modular strategy that allowed rational regulation of enzyme ratios in the co-immobilized system to match cascade reaction requirements, thereby promoting optimal yield. This GOx-LIP dual-enzyme reactor achieved alkene epoxidation with epoxide yields exceeding 70% under optimized conditions. Notably, the yield could be further enhanced to 90% by incorporating oxidized nicotinamide adenine dinucleotide as a cofactor. This modular biological platform demonstrates the superiority of multi-enzyme cascade catalysis in alkene epoxidation.
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Arabian Journal of Chemistry
·
2021
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IF 5.165
Kun Zhou,Boran Wu,Xiaoli Chai,Xiaohu Dai
A novel absorbent, PVA/SA-CFZ, was developed by jointly enveloping nanostructured hydrated ferric-zirconium binary oxide and clinoptilolite in the cross-linking of polyvinyl alcohol (PVA)/sodium alginate (SA). As a result, the cation exchange capacity of clinoptilolite and electrostatic attraction capacity of hydrated ferric-zirconium binary oxide can be integrated for the simultaneous removal of nitrogen and phosphorus from wastewater. The adsorption behavior of PVA/SA-CFZ for the co-existing ammonium and phosphate was primarily investigated by batch experiments. Over a wide pH range (4 ~ 11), PVA/SA-CFZ always showed the excellent adsorption performance both for the low-concentration ammonium (<5 mg/L) and phosphate (<0.5 mg/L), which reflected the well adaptability towards unfavorable environmental conditions. The kinetic analysis indicated that the pseudo-second-order kinetic model could quantitatively describe the adsorption process optimally. The mechanistic investigation revealed that ammonium was captured through the cation exchange reaction with clinoptilolite. The electrostatic attraction, inner sphere complexation with Fe/Zr-O-OH groups, and formation of phosphate precipitation jointly contributed to the fixation of phosphate by PVA/SA-CFZ. Generally, it is believed that PVA/SA-CFZ can serve as a promising composite absorbent for the simultaneous deep removal of nitrogen and phosphorus, and support for the advanced treatment of wastewater containing low-level nutrients.
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JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
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2020
·
IF 1.992
Jin Xu,Xuejia Shi,Xiaomeng Zhang,Zhenzhong Wang,Wei Xiao,Linguo Zhao
To efficiently recycle GH78 thermostable rhamnosidase (TpeRha) and easily separate it from the reaction mixture and furtherly improve the enzyme properties, the magnetic particle Fe 3 O 4 -SiO 2 -NH 2 -Cellu-ZIF8 (FSNcZ8) was prepared by modifying Fe 3 O 4 -NH 2 with tetraethyl silicate (TEOS), microcrystalline cellulose and zinc nitrate hexahydrate. FSNcZ8 displayed better magnetic stability and higher-temperature stability than unmodified Fe 3 O 4 -NH 2 (FN), and it was used to adsorb and immobilize TpeRha from Thermotoga petrophilea 13995. As for properties, FSNcZ8-TpeRha showed optimal reaction temperature and pH of 90°C and 5.0, while its highest activity approached 714 U/g. In addition, FSNcZ8-TpeRha had better higher-temperature stability than FN. After incubation at 80°C for 3 h, the residual enzyme activities of FSNcZ8-TpeRha, FN-TpeRha and free enzyme were 93.5%, 63.32%, and 62.77%, respectively. The organic solvent tolerance and the monosaccharides tolerance of FSNcZ8-TpeRha, compared with free TpeRha, were greatly improved. Using naringin (1 mmol/l) as the substrate, the optimal conversion conditions were as follows: FSNcZ8-TpeRha concentration was 6 U/ml; induction temperature was 80°C; the pH was 5.5; induction time was 30 min, and the yield of products was the same as free enzyme. After repeating the reaction 10 times, the conversion of naringin remained above 80%, showing great improvement of the catalytic efficiency and repeated utilization of the immobilized α-L-rhamnosidase.
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TALANTA
·
2019
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IF 4.916
Chenlu Wang,Wei Zhou,Xiaoyan Liao,Wenqing Li,Zilin Chen
The application of stir bar sorptive extraction (SBSE) for polar compounds has been restricted because of less choice of suitable sorbents . In this study, an ionic liquid-based organic polymer with desired anionic exchange ability and porous structure was used for extraction of polar analytes with high extraction efficiency and capacity. For improving the workability of stir bar, the polymer was immobilized onto chemically resistant poly(ether ether ketone) (PEEK) tube by covalent modification method which including a skillful functionalization process. Afterwards, a facile detachable dumbbell-shaped structure with modified PEEK tube as jacket was constructed. Due to the excellent chemical and physical strength of PEEK material and dumbbell-shaped structure, the stir bar performed remarkable stability in the SBSE process. In addition, the detachable structure allowed the elution can be accomplished directly in the insert tube of autosampler vial with small amounts of solution, which significantly enhanced the enrichment factor and simplified the operation. After coupling with HPLC-MS/MS, the prepared organic polymer modified stir bar was applied for the analysis of three chlorophenoxy acid herbicides and showed favorable extraction efficiency with enrichment factors between 198 and 206 folds. For quantitative analysis of three chlorophenoxy acid herbicides, the method has wide linear range (0.003–10 ng/mL), good linearity (R 2 ≥ 0.9958) and good reproducibility (intra-day relative standard deviation ≤ 6.16%). Furthermore, the proposed method was applied to determine chlorophenoxy acid herbicides in soil samples, the recoveries ranged from 79.4% to 97.4%, with relative standard deviation ≤ 9.3%.
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MICROCHIMICA ACTA
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2018
·Vol. 185
·期 11
·1-7
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IF 5.705
Jia Haiyan,Tian Qingyun,Xu Jingkun,Lu Limin,Ma Xue,Yu Yongfang
A three-dimensional porous network graphene aerogel (GAs) with large specific area and excellent conductivity was loaded with β-cyclodextrin polymer (Pβ-CD) to serve as a support for immobilization of antibodies. A highly sensitive immunosensor for the cancer marker carbohydrate antigen 15–3 (CA15–3) was designed based on the use of Pβ-CD/GAs. The large specific area of GAs warrants high loading with antibodies, and their excellent electrical conductivity warrants strong electrical signals. Based on the synergistic effect of GAs and Pβ-CD, an immunoassay was designed that is making use of hexacyanoferrate as an electrochemical probe and having a pleasantly low working potential of 0.2 V (vs. SCE). Response is linear in the 0.1 mU mL −1 to 100 U mL −1 activity range, and the lower detection limit is 0.03 mU mL −1 (at S/ N = 3). The immunoassay is stable, selective and reproducible. It was applied to the analysis of spiked samples, and results were satisfactory.
Schematic of an electrochemical immunoassay for the carbohydrate antigen 15–3. It is based on the use of β-cyclodextrin polymer and a graphene aerogel.
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CHEMICAL ENGINEERING JOURNAL
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2025
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IF 13.2
Miaoling Chen,Yu Chen,Zhengyuan Zhou,Purui Lin,Minhua Su,Jiacheng Min,Kaimin Shih,Ying Zhou
Radioactive strontium (Sr 2+ ) and cobalt (Co 2+ ) in nuclear wastewater pose significant environmental risks, leading the need of developing effective remediation strategies. In this study, a poly (triazine imide) (PTI) composite intercalated with bromide (Br − ), referred to as PTI/LiBr, synthesized via a molten salt method, is introduced as a novel adsorbent for the removals of Sr 2+ and Co 2+ . PTI/LiBr composite exhibited rapid adsorption and high efficiency for both radionuclides, driven by electrostatic interactions and partial exchange of Li + ions. The removal efficiency for Sr 2+ and Co 2+ exceeded 95 % within 30 min. Adsorption kinetics followed a pseudo-second-order model, while isotherm analysis indicated that Sr 2+ adsorption aligned with the Freundlich model and Co 2+ adsorption followed the Langmuir model. The adsorption process was confirmed to be spontaneous and endothermic. The γ-radiation tolerance tests verified the structural stability and high adsorption efficiency of the material under ionizing radiation conditions. Additionally, DFT-based wave function and charge density analyses showed a stronger and more stable interaction between PTI/LiBr and Co 2+ compared to Sr 2+ , which might be driven by favorable magnetic interactions and significant charge transfer. This study highlights the potential of PTI/LiBr as a robust and efficient adsorbent for mitigating radioactive pollutants in complex aqueous environments.
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ANALYTICAL CHEMISTRY
·
2018
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IF 6.042
Yayun Hong,Hongli Zhao,Chenlu Pu,Qiliang Zhan,Qianying Sheng,Minbo Lan
In this work, multifunctional Ti4+-immobilized phytic acid-modified magnetic graphene (denoted as MagG@PEI@PA-Ti4+) nanocomposites were fabricated through a facile route for simultaneous/respective enrichment of N-glyco- and phosphopeptides. Phytic acid (PA), with six phosphate groups, possesses excellent hydrophilicity and metal ion coordination ability, which endowed the MagG@PEI@PA-Ti4+ with combined properties of immobilized metal ion affinity chromatography (IMAC)- and hydrophilic interaction liquid chromatography (HILIC)-based materials. On the basis of the different binding ability of N-glyco- and phosphopeptides on MagG@PEI@PA-Ti4+, the MagG@PEI@PA-Ti4+ nanocomposites could enrich N-glyco- and phosphopeptides simultaneously or respectively by using different enrichment conditions, achieving controllable selective enrichment of N-glyco- and phosphopeptides. The proposed nanocomposites demonstrated an outstanding performance for selective enrichment of N-glycopeptides (selectivity, 1:1000 molar ratios of IgG/BSA; sensitivity, 0.5 fmol/μL IgG; loading capacity, 300 mg g–1; recovery, >90%) and phosphopeptides (selectivity, 1:5000 molar ratios of α-casein/BSA; sensitivity, 0.1 fmol/μL α-casein; loading capacity, 100 mg g–1; recovery, >90%). Taking advantage of these merits, a total of 393 N-glycopeptides derived from 259 glycoproteins and 574 phosphopeptides derived from 341 phosphoproteins were identified from 200 μg of HeLa cell extracts through a single-step enrichment using MagG@PEI@PA-Ti4+.
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BIORESOURCE TECHNOLOGY
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2012
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IF 4.98
Xiaoyan Wang,Zuoming Zhou,Guohua Jing
Magnetic poly(styrene–glycidyl methacrylate) porous microspheres (MPPM) with high magnetic contents were prepared by surfactant reverse micelles and emulsion polymerization of monomers, in which the well-dispersed Fe 3 O 4 nanoparticles were modified by polyethylene glycol (PEG) and oleic acid (OA) respectively. The characterizations showed that both of the OA–MPPM and the PEG–MPPM were ferromagnetic, however, the OA–MPPM was used to immobilize the bacteria for more advantages. Therefore, the effects of monomer ratio, surfactant, crosslinker and amount of Fe 3 O 4 on the structure, morphology and magnetic contents of the OA–MPPM were investigated. Then, the OA–MPPM was utilized to immobilize Klebsiella sp. FD-3, an iron-reducing bacterium for Fe(III)EDTA reduction applied in NO x removal. Compared with free bacteria, the immobilized FD-3 showed a better tolerance to the unbeneficial pH and temperature conditions.
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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2017
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IF 3.671
Mingan Yu,Duqiang Liu,Lili Sun,Jing Li,Qian Chen,Lijun Pan,Jingchuan Shang,Shurong Zhang,Wei Li
Ni 2+ -agarose bead-wrapped multi-enzyme/inorganic hybrid sphere composed of the immobilized enzymes as organic component and NaH 2 PO 4 and NaCl as inorganic component was developed by co-immobilizing extracellular His-tagged 3-quinuclidinone reductases and glucose dehydrogenase without pre-purification. The resulting biocatalysts has 3D porous architectures as confirmed by SEM and FESEM, and it enabled the continuous biotransformation of 3-quinuclidone to (R)-3-quinuclidinol with cofactor regeneration in situ. The 3D porous biocatalysts were formed via three steps: First, immobilization of the His-tagged enzymes directly from the cell lysates supernatant. Next, formation of enzyme aggregates, ribbons and gels. Finally, the enzymes, the formed aggregates/ribbons/gels and salt were incorporated to the foam and then covered the Ni 2+ -agarose bead. The technique made the immobilization of these enzymes effective such that specific enzyme loading of 60.8 mg/g support and enzyme loading efficiency of 92.3% were achieved. As a direct consequence, the biocatalyst catalyzed the conversion of 3-quinuclidinone (204 g/L) to (R)-3-quinuclidinol in 100% yield and 100% ee at 4.5 h, and the recyclability of the biocatalyst was excellent, retaining > 95% conversion yield and 100% ee even after the fifteenth runs. Overall, our strategy is demonstrated to be a promising method for developing efficient and robust biocatalyst for asymmetric synthesis.
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