MICROCHIMICA ACTA
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2021
·Vol. 188
·期 8
·1-10
·
IF 5.833
Liu Jia Li,Ma Yu Chan,Yang Tong,Hu Rong,Yang
Yun Hui
An ultrasensitive electrochemical sensor has been constructed for the detection of single nucleotide polymorphisms (SNPs) based on DNA-functionalized Cd-MOFs-74 as cascade signal amplification probe under enzyme-free conditions. Interestingly, the introduction of an auxiliary probe did not disturb the detection of SNP targets, but could bind more Cd-MOFs-74 signal elements to enhance the different pulse voltammetry electrochemical signal 2~3 times as compared to sensing system without auxiliary probe, which obviously improves the sensitivity of the proposed sensor. Experimental results taking p53 tumor suppressor gene as SNP model demonstrated that the proposed method can be employed to sensitively and selectively detect target p53 gene fragment with a linear response ranging from 0.01 to 30 pmol/L (detection limit of 6.3 fmol/L) under enzyme-free conditions. Utilizing this strategy, the ultrasensitive SNP electrochemical sensor is a promising tool for the determination of SNPs in biomedicine.
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Nature Communications
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2021
·Vol. 12
·期 1
·1-9
·
IF 14.919
Lin Zirui,Shi Hua-Yu,Lin Lu,Yang Xianpeng,Wu Wanlong,Sun Xiaoqi
Rechargeable aqueous zinc-organic batteries are promising energy storage systems with low-cost aqueous electrolyte and zinc metal anode. The electrochemical properties can be systematically adjusted with molecular design on organic cathode materials. Herein, we use a symmetric small molecule quinone cathode, tetraamino-p-benzoquinone (TABQ), with desirable functional groups to protonate and accomplish dominated proton insertion from weakly acidic zinc electrolyte. The hydrogen bonding network formed with carbonyl and amino groups on the TABQ molecules allows facile proton conduction through the Grotthuss-type mechanism. It guarantees activation energies below 300 meV for charge transfer and proton diffusion. The TABQ cathode delivers a high capacity of 303 mAh g −1 at 0.1 A g −1 in a zinc-organic battery. With the increase of current density to 5 A g −1 , 213 mAh g −1 capacity is still preserved with stable cycling for 1000 times. Our work proposes an effective approach towards high performance organic electrode materials.
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JOURNAL OF NANOBIOTECHNOLOGY
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2021
·Vol. 19
·期 1
·1-23
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IF 10.435
Jiang Weixi,Su Lei,Ao Meng,Guo Xun,Cheng Chen,Luo Yuanli,Xie Zhuoyan,Wang Xingyue,Wang Junrui,Liu Shuling,Cao Yang,Li Pan,Wang Zhigang,Ran Haitao,Zhou Zhiyi,Ren Jianli
Background Recent studies have demonstrated that multidrug resistance (MDR) is a critical factor in the low efficacy of cancer chemotherapy. The main mechanism of MDR arises from the overexpression of P-glycoprotein (P-gp), which actively enhances drug efflux and limits the effectiveness of chemotherapeutic agents. Results In this study, we fabricated a “combo” nanoagent equipping with triple synergistic strategies for enhancing antitumor efficacy against MDR cells. Tumor homing-penetrating peptide endows the nanosystem with targeting and penetrating capabilities in the first stage of tumor internalization. The abundant amine groups of polyethylenimine (PEI)-modified nanoparticles then trigger a proton sponge effect to promote endo/lysosomal escape, which enhances the intracellular accumulation and retention of anticancer drugs. Furthermore, copper tetrakis(4-carboxyphenyl)porphyrin (CuTCPP) encapsulated in the nanosystem, effectively scavenges endogenous glutathione (GSH) to reduce the detoxification mediated by GSH and sensitize the cancer cells to drugs, while simultaneously serving as a photoacoustic imaging (PAI) contrast agent for image visualization. Moreover, we also verify that these versatile nanoparticles in combination with PD-1/PD-L1 blockade therapy can not only activate immunological responses but also inhibit P-gp expression to obliterate primary and metastatic tumors. Conclusion This work shows a significant enhancement in therapeutic efficacy against MDR cells and syngeneic tumors by using multiple MDR reversing strategies compared to an equivalent dose of free paclitaxel. Graphic Abstract
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JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY
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2021
·Vol. 329
·期 3
·1467-1476
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IF 1.371
Yan Meng,Cheng Wencai,Liu Yi,He Xiyang,Wu Biao,Zhang Zhentao,Xie Yi,Lu Xirui
A novel pretreatment method was used to ameliorate the treatment effect of iodine contained radioactive waste. The pretreatment sample (15 w%) held the highest amorphous index (0.50) at 600 °C, and iodine element was in a state of uniform distribution. The density of the sample could increase to 2.95 gcm 3 by using the novel method, which was higher than other similar studies. The leaching result of iodine was as low as 1.53 × 10 6 gm 2 d 1 after 7 days treatment, which indicated that the solidified body has good stability in a long run. Graphic abstract
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JOURNAL OF NANOBIOTECHNOLOGY
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2021
·Vol. 19
·期 1
·1-16
·
IF 10.435
Xiao Jianmin,Yan Miao,Zhou Ke,Chen Hui,Xu Zhaowei,Gan Yuehao,Hong Biao,Tian Geng,Qian Junchao,Zhang Guilong,Wu Zhengyan
The cell membrane is widely considered as a promising delivery nanocarrier due to its excellent properties. In this study, self-assembled Pseudomonas geniculate cell membranes were prepared with high yield as drug nanocarriers, and named BMMPs. BMMPs showed excellent biosafety, and could be more efficiently internalized by cancer cells than traditional red cell membrane nanocarriers, indicating that BMMPs could deliver more drug into cancer cells. Subsequently, the BMMPs were coated with nanoselenium (Se), and subsequently loaded with Mn2+ ions and doxorubicin (DOX) to fabricate a functional nanoplatform (BMMP-Mn2+/Se/DOX). Notably, in this nanoplatform, Se nanoparticles activated superoxide dismutase-1 (SOD-1) expression and subsequently up-regulated downstream H2O2 levels. Next, the released Mn2+ ions catalyzed H2O2 to highly toxic hydroxyl radicals (·OH), inducing mitochondrial damage. In addition, the BMMP-Mn2+/Se nanoplatform inhibited glutathione peroxidase 4 (GPX4) expression and further accelerated intracellular reactive oxygen species (ROS) generation. Notably, the BMMP-Mn2+/Se/DOX nanoplatform exhibited increased effectiveness in inducing cancer cell death through mitochondrial and nuclear targeting dual-mode therapeutic pathways and showed negligible toxicity to normal organs. Therefore, this nanoplatform may represent a promising drug delivery system for achieving a safe, effective, and accurate cancer therapeutic plan.
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CHINESE JOURNAL OF POLYMER SCIENCE
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2021
·Vol. 39
·期 12
·1609-1616
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IF 3.603
Wang Meng,Chu Da,Liu Li,Huang Shuai,Chen Xu-Man,Liu Zhi-Yang,Yang Hong
Reversible switching from a highly rough surface to another entirely smooth surface under external stimuli is crucial for intelligent materials applied in the fields of anti-fogging, self-cleaning, oil-water separation and biotechnology. In this work, a thermal-responsive liquid crystal elastomer (LCE) surface covered with oriented micropillars is prepared via a facile two-step crosslinking method coupled with an extrusion molding program. The reversible change of topological structures of the LCE surface along with temperature is investigated by metallographic microscope, atomic force microscopy and optical contact angle measuring system. At room temperature, the LCE sample is filled with plenty of micropillars with an average length of 8.76 µm, resulting in a super-hydrophobic surface with a water contact angle (WCA) of 135°. When the temperature is increased to above the clearing point, all the micropillars disappear, the LCE surface becomes entirely flat and presents a hydrophilic state with a WCA of 64°. The roughness-related wetting property of this microstructured LCE surface possesses good recyclability in several heating/cooling cycles. This work realizes a truly reversible transformation from a highly rough surface to an entirely smooth surface, and might promote the potential applications of this dynamic-responsive LCE surface in smart sensors and biomimetic control devices.
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Journal of Bionic Engineering
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2021
·Vol. 18
·期 3
·559-573
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IF 2.682
Zhang Ruilong,Zhou Zhiping,Wang Yi,Dai Xiaohui,Chen Li,Dai Jiangdong
Mimicry of nature drives the development of bionic materials. Bionic superhydrophobic materials are a kind of high-efficiency materials to handle oil spills and water pollution. However, the stability of surface coatings of the superhydrophobic materials remains a challenge. Herein, a new category of self-assembly bionic superhydrophobic surface coating was prepared via one-step condensation/copolymerization of vinyltriethoxysilane (VTES) and divinylbenzene (DVB), which realized the close combination of covalent bonds between organic ( e.g. DVB) and inorganic matter ( e.g. VTES), and avoided the swelling of polydivinylbenzene (PDVB) in the process of collection of oil from water. This organic-inorganic hybrid polymer could self-assembly deposit on the surface of sponge even other substrates. For example, P(VTES-DVB)-SiO 2 /MS obtained by assembling P(VTES-DVB)-SiO 2 on the surface of Melamine Sponge (MS) exhibited superhydrophobicity with a Water Contact Angle (WCA) of 157.3°, the optimal adsorption capacity of 77 g·g −1 − 136 g·g −1 for diverse oils, and an excellent separation efficiency of 99.3%. Besides, the excellent acid and alkali resistance of P(VTES-DVB)-SiO 2 /MS suggested the potential value in practical oil-water separation. P(VTES-DVB)-SiO 2 showed the outstanding hydrophobic performance by using as coating on different substrates. This work provided a new idea about the stable combination of organic and inorganic matter in the surface modification.
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Science China-Technological Sciences
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2021
·Vol. 65
·期 1
·179-190
·
IF 3.572
Qi YuHao,Zheng JunPing
A wearable UV sensor is designed to realize UV detection and a warning effect on people’s excessive UV exposure. It is noteworthy that the photoelectric system and supporting material of the most conventional sensor are separated. The unstable connection between the two components and the complicated construction method makes the sensor susceptible to external motion interference and prone to failure. Herein, we developed a unique photo response mode of the UV sensor based on a novel photo responsive material. An azobenzene-containing polydimethylsiloxane (Azo-PDMS) film was prepared as the outer layer of the sensor. It integrated the functions of photo response source, support and protection, which realized the direct contact and rapid response to the UV light source. Carbon nanotube (CNT), wrapped in the middle of Azo-PDMS films as an inner layer, transformed the photomechanical response signal into the photoelectric signal. The photo response mode endows the sensor with excellent anti-motion interference capabilities and a true sense of UV-strain synchronous monitoring performance, which is of great significance to the practical application of wearable devices. In addition, based on the excellent properties of Azo-PDMS, the sensor can also protect the human body from UV damage, self-repair after fracture, and realize personalized customization through 3D printing. It makes a breakthrough in the design and construction of wearable UV sensors and paves a new way to optimize sensor photo response modes.
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JOURNAL OF NANOBIOTECHNOLOGY
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2021
·Vol. 19
·期 1
·1-14
·
IF 10.435
Guo Xiaoyang,Liu Jia,Jiang Lingdong,Gong Wanjun,Wu Huixia,He Qianjun
Background Photothermal therapy (PTT) frequently cause thermal resistance in tumor cells by inducing the heat shock response, limiting its therapeutic effect. Hydrogen sulfide (H2S) with appropriate concentration can reverse the Warburg effect in cancer cells. The combination of PTT with H2S gas therapy is expected to achieve synergistic tumor treatment.Methods Here, sulourea (Su) is developed as a thermosensitive/hydrolysable H2S donor to be loaded into Pd nanocubes through in-depth coordination for construction of the Pd-Su nanomedicine for the first time to achieve photo-controlled H2S release, realizing the effective combination of photothermal therapy and H2S gas therapy.Results The Pd-Su nanomedicine shows a high Su loading capacity (85 mg g−1), a high near-infrared (NIR) photothermal conversion efficiency (69.4%), and NIR-controlled H2S release by the photothermal-triggered hydrolysis of Su. The combination of photothermal heating and H2S produces a strong synergetic effect by H2S-induced inhibition of heat shock response, thereby effectively inhibiting tumor growth. Moreover, high intratumoral accumulation of the Pd-Su nanomedicine after intravenous injection also enables photothermal/photoacoustic dual-mode imaging-guided tumor treatment.Conclusion sThe proposed NIR-responsive heat/H2S release strategy provides a new approach for effective cancer therapy.Graphic abstract
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Nanoscale Research Letters
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2021
·Vol. 16
·期 1
·1-16
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IF 4.703
Zhou Lin,Huang Zhi,Yang Shanyi,Wei Jiarui,Xu Yan,Hu Lin,Guo Xinrong,Yuan Limin,Yuan Zexuan,Yang Xiaoping,Tao Xiaojun,Zhang Qiufang
Hydrophilic polyethylene glycol monomethyl ether (mPEG) was grafted onto Icariin (ICA) by succinic anhydride to form a polyethylene glycol-Icariin (mPEG-ICA) polymer. The structure of the polymer was characterized by Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR). mPEG-ICA nanoparticles loaded with ICA were prepared by physical embedding of ICA by dialysis. The particle size was determined to be (220 ± 13.7) nm, and the ζ potential was (2.30 ± 1.33) mV by dynamic light scattering (DLS). Under a transmission electron microscope (TEM), the nanoparticles were spherical, and the morphology was regular. In the medium with pH 7.4, the drug release rate of mPEG-ICA nanoparticles reached (52.80 ± 1.70)% within 72 h. At pH 6.8, the cumulative drug release of nanoparticles reached (75.66 ± 0.17)% within 48 h. Treatment of the nanoparticles with LPS-treated H9c2 cells maintained cell viability, reduced LDH release and exerted antiapoptotic effects. Moreover, ICA-loaded mPEG-ICA nanoparticles significantly decreased the mRNA expression of the myocardial inflammatory cytokines TNF-α, IL-1β and IL-6M. In conclusion, ICA-loaded mPEG-ICA nanoparticles protected against LPS-induced H9c2 cell injury.
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Nature Communications
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2021
·Vol. 12
·期 1
·1-11
·
IF 14.919
Long Yanping,Li Ling,Xu Tao,Wu Xizheng,Gao Yun,Huang Jianbo,He Chao,Ma Tian,Ma Lang,Cheng Chong,Zhao Changsheng
Pathogenic drug-resistant bacteria represent a threat to human health, for instance, the methicillin-resistant Staphylococcus aureus (MRSA). There is an ever-growing need to develop non-antibiotic strategies to fight bacteria without triggering drug resistance. Here, we design a hedgehog artificial macrophage with atomic-catalytic centers to combat MRSA by mimicking the “capture and killing” process of macrophages. The experimental studies and theoretical calculations reveal that the synthesized materials can efficiently capture and kill MRSA by the hedgehog topography and substantial generation of •O 2 − and HClO with its Fe 2 N 6 O catalytic centers. The synthesized artificial macrophage exhibits a low minimal inhibition concentration (8 μg/mL Fe-Art M with H 2 O 2 (100 μM)) to combat MRSA and rapidly promote the healing of bacteria-infected wounds on rabbit skin. We suggest that the application of this hedgehog artificial macrophage with “capture and killing” capability and high ROS-catalytic activity will open up a promising pathway to develop antibacterial materials for bionic and non-antibiotic disinfection strategies.
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PLANTA
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2021
·Vol. 254
·期 5
·1-10
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IF 4.116
Wang Jiadian,Su Ping,Gao Linhui,Zhang Yifeng,Wang Jian,Tu Lichan,Zhao Yujun,Lu Yun,Yin Yan,Huang Luqi,Gao Wei
Main conclusion A novel cytochrome P450 from Tripterygium wilfordii , CYP81AM1, specifically catalyses the C-15 hydroxylation of dehydroabietic acid. This is the first CYP450 to be found in plants with this function. Abstract Cytochrome P450 oxygenases (CYPs) play an important role in the post-modification in biosynthesis of plant bioactive terpenoids. Here, we found that CYP81AM1 can catalyze the formation of 15-hydroxydehydroabietic acid by in vitro enzymatic reactions and in vivo yeast feeding assays. This is the first study to show that CYP81 family enzymes are involved in the hydroxylation of abietane diterpenoids. At the same time, we found that CYP81AM1 could not catalyse abietatriene and dehydroabietinol, suggesting that the occurrence of the reaction may be related to the carboxyl group. Through molecular docking and site mutations, it was found that some amino acid sites (F104, K107) near the carboxyl group had an important effect on enzyme activity, also suggesting that the carboxyl group played an important role in the occurrence of the reaction.
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