JOURNAL OF HAZARDOUS MATERIALS
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2016
·
IF 4.836
Ting Liu,Jiang Xue Dong,Shi Gang Liu,Na Li,Shu Min Lin,Yu Zhu Fan,Jing Lie Lei,Hong Qun Luo,Nian Bing Li
A stable silver nanoparticles/carbon quantum dots (Ag/CQDs) composite was prepared by using CQDs as reducing and stabilizing agent. The CQDs synthesized with polyethyleneimine (PEI) showed an extraordinary reducibility. When Hg 2+ was presented in the Ag/CQDs composite solution, a color change from yellow to colorless was observed, accompanied by a shift of surface plasmon resonance (SPR) band and decrease in absorbance of the Ag/CQDs composite. On the basis of the further studies on TEM, XPS and XRD analysis, the possible mechanism is attributed to the formation of a silver-mercury amalgam. Hence, a two dimensional sensing platform for Hg 2+ detection was constructed upon the Ag/CQDs composite. Based on the change of absorbance, a good linear relationship was obtained from 0.5 to 50 μM for Hg 2+ . And the limit of detection for Hg 2+ was as low as 85 nM, representing high sensitivity to Hg 2+ . More importantly, the proposed method also exhibits a good selectivity toward Hg 2+ over other metal ions. Besides, this strategy demonstrates practicability for the detection of Hg 2+ in real water samples with satisfactory results.
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Analytical Methods
·
2016
·Vol. 8
·期 43
·7755-7761
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IF 1.915
Cong Wang,Junyong Sun,Han Mei,Feng Gao
In this work, the synthesis of carboxyl functionalized semiconducting polymer dots (PFBT-COOH Pdots) is achieved by a co-precipitation method. The PFBT-COOH Pdots show extraordinary fluorescence brightness and excellent photostability, and the fluorescence of PFBT-COOH Pdots is selectively quenched by ferric ions. Upon addition of phytic acid (PA) to the Pdots/Fe(III) system, PA is able to selectively and preferentially bind with the ferric ions, thus leading to fluorescence recovery of the PFBT-COOH Pdots. Therefore, a simple, selective and sensitive fluorescence probe for PA detection is designed based on the fluorescence quenching-recover process. This probe exhibits a good linear relationship (R = 0.9988) with a linear range from 2.5 μmol L−1 to 450 μmol L−1 and a limit of detection of 0.63 μmol L−1. The probe also shows good selectivity for PA and a good recovery ratio, and it is applied to the determination of PA in a corn grains sample with satisfactory results.
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TALANTA
·
2016
·
IF 4.035
Lijiang Lei,Jin Zhu,Gangqiang Xia,Hui Feng,Hongman Zhang,Yuwang Han
NGAL is a promising novel biomarker for acute kidney injury (AKI) and chronic kidney disease (CKD). More rapid and user-friendly methods are needed for the timely monitoring of NGAL in human urine and serum. UCP technology-based lateral flow assay (UPT-LFA) was developed for rapid, user-friendly and quantitative detection of the NGAL in human serum specimens and urine specimens. Under optimal conditions, the UPT-LFA displayed a rapid response to NGAL with a LOD of 7.68 ng/mL and detection range from 7.68 to 1000 ng/mL. The UPT-LFA method was compared with commercial immunoturbidimetry (103 urine specimens), and by ELISA (26 serum specimens), respectively. The results demonstrated that the UPT-LFA was consistent with immunoturbidimetry assay, reporting a 97.92% of positive and 92.73% of negative coincidence rates, respectively. Meanwhile, the concordance rate between UPT-LFA and ELISA, as shown by correlative regression analysis, was also high (R 2 =0.95). The whole assay can be completed within 30 min compared to 4 h consuming with ELISA. The research implies that, the UPT-LFA provides a potential to be used in point of care testing (POCT) to define early acute kidney injury with advantages of user-friendly and rapid testing, promising this new assay a bright future.
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Materials Science & Engineering C-Materials for Biological Applications
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2017
·
IF 4.164
Hongxia Liu,Shuqin Wu,Jingmou Yu,Dun Fan,Jin Ren,Lei Zhang,Jianguo Zhao
Reduction-sensitive chondroitin sulfate A (CSA)-based micelles were developed. CSA was conjugated with deoxycholic acid (DOCA) via a disulfide linkage. The bioreducible conjugate (CSA-ss-DOCA) can form self-assembled micelles in aqueous medium. The critical micelle concentration (CMC) of CSA-ss-DOCA conjugate is 0.047 mg/mL, and its mean diameter is 387 nm. The anticancer drug doxorubicin (DOX) was chosen as a model drug, and was effectively encapsulated into the micelles with high loading efficiency. Reduction-sensitive micelles and reduction-insensitive control micelles displayed similar DOX release behavior in phosphate buffered saline (PBS, pH 7.4). Notably, DOX release from the reduction-sensitive micelles in vitro was accelerated in the presence of 20 mM glutathione-containing PBS environment. Moreover, DOX-loaded CSA-ss-DOCA (CSA-ss-DOCA/DOX) micelles exhibited intracellular reduction-responsive characteristics in human gastric cancer HGC-27 cells determined by confocal laser scanning microscopy (CLSM). Furthermore, CSA-ss-DOCA/DOX micelles demonstrated higher antitumor efficacy than reduction-insensitive control micelles in HGC-27 cells. These results suggested that reduction-sensitive CSA-ss-DOCA micelles had the potential as intracellular targeted carriers of anticancer drugs.
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Catalysts
·
2017
·Vol. 7
·期 11
·327
·
IF 3.082
Hao Liang,Shanshan Sun,Yan Zhou,Yanhui Liu
In this study, a one-step and facile immobilization of enzymes by self-assembly of zinc ions and adenine in aqueous solution with mild conditions was reported. Enzymes, such as glucose oxidase (GOx) and horseradish peroxidase (HRP), could be efficiently encapsulated in Zn/adenine coordination polymers (CPs) with high loading capacity over 90%. When the enzyme was immobilized by CPs, it displayed high catalytic efficiency, high selectivity and enhanced stability due to the protecting effect of the rigid framework. As a result, the relative activity of Zn/adenine nano-CP-immobilized GOx increased by 1.5-fold at pH 3 and 4-fold at 70 to 90 °C, compared to free GOx. The immobilized GOx had excellent reusability (more than 90% relative activity after being reused eight times). Furthermore, the use of this system as a glucose biosensor was also demonstrated by co-immobilization of two enzymes, detecting glucose down to 1.84 µM with excellent selectivity. The above work indicated that in-situ self-assembly of Zn/adenine CPs could be a simple and efficient method for biocatalyst immobilization.Keywords:adenine;enzyme;self-assembly;coordination polymers;immobilization
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MOLECULES
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2017
·Vol. 22
·期 1
·179
·
IF 2.861
Chunfang Li,Shuhui Jiang,Xinying Zhao,Hao Liang
In this paper we report a facile method for preparing co-immobilized enzyme and magnetic nanoparticles (MNPs) using metal coordinated hydrogel nanofibers.Candida rugosalipase (CRL) was selected as guest protein. For good aqueous dispersity, low price and other unique properties, citric acid-modified magnetic iron oxide nanoparticles (CA-Fe3O4NPs) have been widely used for immobilizing enzymes. As a result, the relative activity of CA-Fe3O4@Zn/AMP nanofiber-immobilized CRL increased by 8-fold at pH 10.0 and nearly 1-fold in a 50 °C water bath after 30 min, compared to free CRL. Moreover, the immobilized CRL had excellent long-term storage stability (nearly 80% releative activity after storage for 13 days). This work indicated that metal-nucleotide nanofibers could efficiently co-immobilize enzymes and MNPs simultaneously, and improve the stability of biocatalysts.Keywords:metal;nucleotide;magnetic iron oxide nanoparticles;Candida rugoselipase (CRL);immobilized enzyme
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ACS Applied Materials & Interfaces
·
2017
·
IF 7.504
Hao Liang,Feifei Lin,Zijie Zhang,Biwu Liu,Shuhui Jiang,Qipeng Yuan,Juewen Liu
Using nanomaterials to achieve functional enzyme mimics (nanozymes) is attractive for both applied and fundamental research. Laccases are multicopper oxidases highly important for biotechnology and environmental remediation. In this work, we report an exceptionally simple yet functional laccase mimic based on guanosine monophosphate (GMP) coordinated copper. It forms an amorphous metal–organic framework (MOF) material. The ratio of copper and GMP is 3:4 as determined by isothermal titration calorimetry. It has excellent laccase-like activity and converts a diverse range of phenol containing substrates such as hydroquinone, naphthol, catechol and epinephrine. Comparative work shows that the activity is originated from guanosine coordination instead of phosphate binding in GMP. Cu2+ is required and cannot be substituted by other metal ions. At the same mass concentration, the Cu/GMP nanozyme has a higher Vmax and similar Km compared to the protein laccase. To achieve the same catalytic efficiency, the cost of the Gu/GMP is ∼2400-fold lower than that of laccase. The Cu/GMP is much more stable at extreme pH, high salt, high temperature and for long-term storage. This is one of the first laccase-mimicking nanozymes, which will find important applications in analytical chemistry, environmental protection, and biotechnology.
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ANALYST
·
2017
·Vol. 142
·期 22
·4240-4246
·
IF 3.885
Huihui Li,Jin Shen,Rongwei Cui,Chongmei Sun,Yanyan Zhao,Xia Wu,Na Li,Bo Tang
Highly selective determination of dopamine (DA) over other catecholamines is an urgent need for the precise diagnosis and therapy of DA related diseases. Herein, a new formate-bridged Tb(III)-complex and silver nanoparticles (AgNPs) enhanced fluorescent nanosensor was constructed. HCOO− acted as a co-ligand of Tb(III) and also as a linker between the Tb(III) complex and AgNPs and more readily combined with the primary amine of DA than with epinephrine (EP). The formate-bridged action strengthened AgNPs-based surface enhanced fluorescence of the Tb3+-DA complex and improved the selectivity towards DA. Under neutral buffer conditions, the detection limit for the assay of DA was down to 0.15 nM (S/N = 3) with a linear range from 0.5 nM to 100 nM (R2 = 0.9978). Furthermore, the nanosensor could successfully distinguish DA from EP.
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HYDROMETALLURGY
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2016
·
IF 2.29
Junlian Wang,Meiying Xie,Huajun Wang,Shengming Xu
Nonsymmetric (2,3-dimethylbutyl)(2,4,4′-trimethylpentyl)phosphinic acid (INET-3) may be a superior extractant for extraction and separation of heavy rare earths (REs), as its extraction ability falls between that of P507 and Cyanex 272. Its purity and pK a were determined using potentiometric titration methods. The extraction mechanism and extraction equilibrium constants of INET-3 for heavy REs from chloride media were studied. The effects of the anion types (Cl − , NO 3 − and SO 4 2 − ) and concentrations (0.1, 0.5 and 1.0 mol/L) on RE(III) extraction were investigated. The extraction capacity and separation factors of INET-3 for heavy REs were determined. The stripping behaviours of heavy RE complexes with INET-3 by HCl , HNO 3 and H 2 SO 4 were examined. The results showed that the pK a of INET-3 was 5.45. The extraction capacity of INET-3 was approximately twice that of Cyanex 272 and only one-third that of P507. Loaded heavy RE(III) extracted by INET-3 was completely stripped out by HCl, HNO 3 and H 2 SO 4 solutions when the pH was at or below 1.2. Among the three inorganic acids , H 2 SO 4 was the most effective stripping agent. INET-3 can separate heavy RE(III) efficiently, and the separation factors of adjacent heavy RE(III) β Er/Ho , β Tm/Er , β Yb/Tm and β Lu/Yb were 3.25, 2.73, 2.96 and 1.53, respectively, when extracted from a Ho/Er/Tm/Yb/Lu quinary system.
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INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
·
2024
·
IF 3.8
Hongbin Ju,Lizhi Zhang,Heqin Guo,Litao Jia,Bo Hou,Debao Li,Tao Geng
The catalytic epoxidation of α-olefin with long carbon chains has always been a challenge. Traditional homogeneous or heterogeneous catalysts showed poor performance toward the reaction. In this paper, layered organic–inorganic catalysts were prepared by the reaction of phosphotungstic acid and a Gemini quaternary ammonium salt, which could convert long α-olefin to epoxide with a selectivity of 85% in the absence of organic solvents. Combined with characterization results, it was confirmed that the catalyst was layered structure materials, which was supported by a quaternary ammonium salt chain, and the active molecules were distributed between the layered structures. The possible mechanism was also discussed preliminarily.
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JOURNAL OF POLYMERS AND THE ENVIRONMENT
·
2024
·
IF 4.7
Hu Haotian,Liu Hongjing,Li Qingqing,Shen Yongpeng,Lu Jialin,Liu Xiaowen,Tong Yu,Feng Yiheng,Xu Qingbo,Tang Yuxiang,Chu Jing,Hu Hang,Zhu Tianyu,Xu Defeng
Polymer-based nanoparticles with tumor-targeting ability, controlled-release properties and good biocompatibility are of great interest for anticancer drug delivery. Herein, two amphiphilic reduction-responsive copolymers self-assembled nanoparticles (mPEG-Cys-PCL and mPEG-Ami-PCL) along with their inert counterpart (mPEG-Hex-PCL) were prepared and evaluated. These three copolymers were synthesized by conjugating mPEG and PCL with different linkers and characterized by proton nuclear magnetic resonance spectrometry, flourier transform infrared spectrometry and gel permeation chromatography. Nile red (NR) was loaded into the prepared nanoparticles as a model drug to study the in vitro drug release, cellular uptake amount and biodistribution. Dimethylcurcumin (DMC) was loaded into the prepared nanoparticles to study the in vitro antitumor effect. The results show that NR@mPEG-Cys-PCL and NR@mPEG-Ami-PCL nanoparticles exhibit glutathione (GSH)-triggered drug release and NR@mPEG-Ami-PCL nanoparticles display enhanced GSH-responsiveness as compared to NR@mPEG-Cys-PCL. Moreover, NR@mPEG-Ami-PCL nanoparticles possess enhanced cellular uptake amount as compared to NR@mPEG-Hex-PCL and NR@mPEG-Cys-PCL nanoparticles. DMC@mPEG-Ami-PCL nanoparticles possess the highest in vitro antitumor effect. In biodistribution study, both NR@mPEG-Cys-PCL and NR@mPEG-Ami-PCL nanoparticles show reduced organ distribution and similar tumor accumulation as compared to NR@mPEG-Hex-PCL nanoparticles. The mPEG-Ami-PCL nanoparticles developed in this work show great potential for anticancer drug delivery.
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EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
·
2024
·
IF 6
Chao Yang,Haojie Cai,Xinying Zhu,Lei Zhang,Jing Li
Xanthine oxidoreductase (XOR) and uric acid transporter 1 (URAT1) are two most widely studied targets involved in production and reabsorption of uric acid, respectively. Marketed drugs almost target XOR or URAT1, but sometimes, single agents might not achieve aim of lowering uric acid to ideal value in clinic. Thus, therapeutic strategies of combining XOR inhibitors with uricosuric drugs were proposed and implemented. Based on our initial work of virtual screening, A and B were potential hits for dual-targeted inhibitors on XOR/URAT1. By docking A / B with XOR/URAT1 respectively, compounds I1-7 were designed to get different degree of inhibition effect on XOR and URAT1, and I7 showed the best inhibitory effect on XOR (IC 50 = 0.037 ± 0.001 μM) and URAT1 (IC 50 = 546.70 ± 32.60 μM). Further docking research on I7 with XOR/URAT1 led to the design of compounds II with the significantly improved inhibitory activity on XOR and URAT1, such as II11 and II15 . Especially, for II15 , the IC 50 of XOR is 0.006 ± 0.000 μM, superior to that of febuxostat (IC 50 = 0.008 ± 0.000 μM), IC 50 of URAT1 is 12.90 ± 2.30 μM, superior to that of benzbromarone (IC 50 = 27.04 ± 2.55 μM). In acute hyperuricemia mouse model, II15 showed significant uric acid lowering effect. The results suggest that II15 had good inhibitory effect on XOR/URAT1, with the possibility for further investigation in in-vivo models of hyperuricemia.
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